LOCUS       NZ_BMVE01000034         3478 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces goshikiensis strain JCM 4640 sequence34, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVE01000034
VERSION     NZ_BMVE01000034.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces goshikiensis
  ORGANISM  Streptomyces goshikiensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 3478)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650555.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:30:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,491
            CDSs (total)                      :: 7,410
            Genes (coding)                    :: 7,286
            CDSs (with protein)               :: 7,286
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 44 of 125
            Pseudo Genes (incomplete)         :: 101 of 125
            Pseudo Genes (internal stop)      :: 9 of 125
            Pseudo Genes (multiple problems)  :: 27 of 125
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:38:35
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 3478
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVE01000034.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..3478
                     /culture_collection="JCM:4640"
                     /db_xref="taxon:1942"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces goshikiensis"
                     /strain="JCM 4640"
                     /submitter_seqid="sequence34"
                     /type_material="type strain of Streptomyces goshikiensis"
     region          1..3478
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..3478
                     /SMILES="C(C)=CC(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..3478
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[33:>3478](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      34..>3478
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS_motif       34..123
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE248_RS36990_0001"
                     /evalue="8.00E-13"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="IE248_RS36990"
                     /protein_end="30"
                     /protein_start="0"
                     /score="40.6"
                     /tool="antismash"
                     /translation="MGHSQGEIAAAVVAGGLSLEDGARVVVLRA"
     aSDomain        34..666
                     /ASF="Methylmalonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE248_RS36990_PKS_AT.1"
                     /evalue="6.00E-71"
                     /label="IE248_RS36990_PKS_AT.1"
                     /locus_tag="IE248_RS36990"
                     /protein_end="211"
                     /protein_start="0"
                     /score="231.2"
                     /specificity="substrate consensus: mmal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="MGHSQGEIAAAVVAGGLSLEDGARVVVLRARLIGRELAGHGGMAS
                     VALPVAVVEERLARWAGRLGVAVVNGPSATVVAGDTDAVAEFVAGCETEGVRARRLPVD
                     YASHSAHVETLAAELEVILADVRPRAGEIPFYSTVEAAAIDTASLDAGYWFGNLRRPVR
                     FQETVEQLLADGFPVFVECGAHPVLTAAVQETAESVGRQVCAVGSLRR"
     aSModule        34..2580
                     /complete
                     /domains="nrpspksdomains_IE248_RS36990_PKS_AT.1"
                     /domains="nrpspksdomains_IE248_RS36990_PKS_DHt.1"
                     /domains="nrpspksdomains_IE248_RS36990_PKS_KR.1"
                     /domains="nrpspksdomains_IE248_RS36990_PKS_PP.1"
                     /locus_tags="IE248_RS36990"
                     /monomer_pairings="mmal -> Me-ccmal"
                     /starter_module
                     /tool="antismash"
                     /type="pks"
     gene            34..>3478
                     /locus_tag="IE248_RS36990"
     CDS             34..>3478
                     /NRPS_PKS="Domain: PKS_AT (0-211). E-value: 6e-71. Score:
                     231.2. Matches aSDomain:
                     nrpspksdomains_IE248_RS36990_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DHt (226-384). E-value: 1e-07.
                     Score: 24.0. Matches aSDomain:
                     nrpspksdomains_IE248_RS36990_PKS_DHt.1"
                     /NRPS_PKS="Domain: PKS_KR (495-664). E-value: 2.1e-26.
                     Score: 84.9. Matches aSDomain:
                     nrpspksdomains_IE248_RS36990_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (763-849). E-value: 8e-31. Score:
                     98.2. Matches aSDomain:
                     nrpspksdomains_IE248_RS36990_PKS_PP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (876-1148). E-value:
                     7.1e-111. Score: 363.0. Matches aSDomain:
                     nrpspksdomains_IE248_RS36990_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012337.5"
                     /locus_tag="IE248_RS36990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_229895811.1"
                     /sec_met_domain="PKS_AT (E-value: 1e-72, bitscore: 231.2,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.5e-15, bitscore:
                     42.6, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 5.3e-140, bitscore:
                     453.0, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 3.9e-96, bitscore:
                     307.6, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGHSQGEIAAAVVAGGLSLEDGARVVVLRARLIGRELAGHGGMAS
                     VALPVAVVEERLARWAGRLGVAVVNGPSATVVAGDTDAVAEFVAGCETEGVRARRLPVD
                     YASHSAHVETLAAELEVILADVRPRAGEIPFYSTVEAAAIDTASLDAGYWFGNLRRPVR
                     FQETVEQLLADGFPVFVECGAHPVLTAAVQETAESVGRQVCAVGSLRREEGGLRRFLTS
                     VVEAFVQGVDVTWPALFDDTGARLVDLPTYPFQHQRYWLESRPSAAVVPSGTQDGLSYE
                     VTWKSLSLPESVRLDGRWLLVVPEDLDADGTRIAHDVEQALTTHGATVHTLALDPATAD
                     FDGRFSRMFEEATDVTGILSLLGLAVGPHPEHGEVPLGVATSLTLTRQALAGFGVPVWV
                     VTRGAVSVVPGEVPETAGAQLWALGRVAALELPDRWGGLIDLPADTDARSVGLAVRALA
                     AGIAAGEDQLAVRPSGAYGRRLVRATARRGRKDWRPQGTVLLAGDLDAVAEPLVRWLLT
                     DGADHVVLADPALTELPAALADVAQRVTTAAAPDLADRAVLAALVTEYAPAMVVVVPPA
                     VELAPLESTSPADLAAAVAAKSATAAHFDALLDGPHAPELVLISSVAGIWGGVRQGAYA
                     VGAAHLDALAARRRARGLPTVSVAWTPWAGSVIADGSAAESMRQYGIAPLEPQTALAEL
                     NRALGSGTGDAAVADVDWEQFLASFTSVRPTVLFDELPEVRRLRQAEAAAMADQAARIG
                     APGGTELARSLRDKPRNAQRKALLELVTAHVAAVLGEGAPDAIDQSRAFKDIGFTSMTA
                     MELRNRLKEATGLTLPASLVFDHPHPRALADHLREELLGEDDAERTDSEPNTPSGPPPA
                     GQDEPIAIIGMACRLPGDVGTPDELWELLETGRDAITDLPVNRGWDVAGLYDPDPDAAG
                     RAYVREGGFLHDAGEFDAGFFGISPREALAMDPQQRIILELAWESFERAGLDPAGRRGS
                     RTGVFMGTNGQHYMPLLQNGSESFDGYLGTGNSASVMSGRISYTLGLEGPALTVDTACS
                     SSLVALHLAVRALRSGECDLALAGGATVMSTPEVLVEFSRQRAVAADGRCKAFSASADG
                     FGPAEGAGVLLVERLSDAVRHGRRVLAVVRGSAVNQDGASN"
     CDS_motif       232..270
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE248_RS36990_0002"
                     /evalue="9.80E-03"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="IE248_RS36990"
                     /protein_end="79"
                     /protein_start="66"
                     /score="9.4"
                     /tool="antismash"
                     /translation="AVVNGPSATVVAG"
     CDS_motif       340..372
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE248_RS36990_0003"
                     /evalue="4.20E-04"
                     /label="PKSI-AT-mM_m6"
                     /locus_tag="IE248_RS36990"
                     /protein_end="113"
                     /protein_start="102"
                     /score="13.0"
                     /tool="antismash"
                     /translation="VDYASHSAHVE"
     CDS_motif       478..525
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE248_RS36990_0004"
                     /evalue="1.70E-05"
                     /label="PKSI-AT-mM_m8"
                     /locus_tag="IE248_RS36990"
                     /protein_end="164"
                     /protein_start="148"
                     /score="16.8"
                     /tool="antismash"
                     /translation="LDAGYWFGNLRRPVRF"
     aSDomain        712..1185
                     /aSDomain="PKS_DHt"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE248_RS36990_PKS_DHt.1"
                     /evalue="1.00E-07"
                     /label="IE248_RS36990_PKS_DHt.1"
                     /locus_tag="IE248_RS36990"
                     /protein_end="384"
                     /protein_start="226"
                     /score="24.0"
                     /tool="antismash"
                     /translation="FVQGVDVTWPALFDDTGARLVDLPTYPFQHQRYWLESRPSAAVVP
                     SGTQDGLSYEVTWKSLSLPESVRLDGRWLLVVPEDLDADGTRIAHDVEQALTTHGATVH
                     TLALDPATADFDGRFSRMFEEATDVTGILSLLGLAVGPHPEHGEVPLGVATSLT"
     aSDomain        1519..2025
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE248_RS36990_PKS_KR.1"
                     /evalue="2.10E-26"
                     /label="IE248_RS36990_PKS_KR.1"
                     /locus_tag="IE248_RS36990"
                     /protein_end="664"
                     /protein_start="495"
                     /score="84.9"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="GTVLLAGDLDAVAEPLVRWLLTDGADHVVLADPALTELPAALADV
                     AQRVTTAAAPDLADRAVLAALVTEYAPAMVVVVPPAVELAPLESTSPADLAAAVAAKSA
                     TAAHFDALLDGPHAPELVLISSVAGIWGGVRQGAYAVGAAHLDALAARRRARGLPTVSV
                     AWTPWA"
     CDS_motif       1882..1980
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE248_RS36990_0005"
                     /evalue="7.00E-11"
                     /label="PKSI-KR_m4"
                     /locus_tag="IE248_RS36990"
                     /protein_end="649"
                     /protein_start="616"
                     /score="34.7"
                     /tool="antismash"
                     /translation="VLISSVAGIWGGVRQGAYAVGAAHLDALAARRR"
     aSDomain        2323..2580
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE248_RS36990_PKS_PP.1"
                     /evalue="8.00E-31"
                     /label="IE248_RS36990_PKS_PP.1"
                     /locus_tag="IE248_RS36990"
                     /protein_end="849"
                     /protein_start="763"
                     /score="98.2"
                     /tool="antismash"
                     /translation="LRDKPRNAQRKALLELVTAHVAAVLGEGAPDAIDQSRAFKDIGFT
                     SMTAMELRNRLKEATGLTLPASLVFDHPHPRALADHLREEL"
     aSDomain        2662..3477
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE248_RS36990_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="7.10E-111"
                     /label="IE248_RS36990_PKS_KS.1"
                     /locus_tag="IE248_RS36990"
                     /protein_end="1148"
                     /protein_start="876"
                     /score="363.0"
                     /tool="antismash"
                     /translation="IAIIGMACRLPGDVGTPDELWELLETGRDAITDLPVNRGWDVAGL
                     YDPDPDAAGRAYVREGGFLHDAGEFDAGFFGISPREALAMDPQQRIILELAWESFERAG
                     LDPAGRRGSRTGVFMGTNGQHYMPLLQNGSESFDGYLGTGNSASVMSGRISYTLGLEGP
                     ALTVDTACSSSLVALHLAVRALRSGECDLALAGGATVMSTPEVLVEFSRQRAVAADGRC
                     KAFSASADGFGPAEGAGVLLVERLSDAVRHGRRVLAVVRGSAVNQDGASN"
     CDS_motif       3145..3192
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE248_RS36990_0006"
                     /evalue="5.60E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE248_RS36990"
                     /protein_end="1053"
                     /protein_start="1037"
                     /score="21.3"
                     /tool="antismash"
                     /translation="GPALTVDTACSSSLVA"
ORIGIN
        1 tggcgttcgt tgggtgtggt gccggatgcg gtggtggggc attcgcaggg ggagatcgct
       61 gcggcggtgg tggcgggtgg cctgtcgttg gaggacgggg cccgtgtcgt ggtgctacgt
      121 gcacggttga tcgggcgtga gctggcgggc catggcggga tggcgtcggt ggcgttgcca
      181 gtggcggtgg tggaggagcg cctggcgcgg tgggcggggc gtctgggtgt ggcggtggtc
      241 aacggcccgt cggccaccgt ggtcgccggt gacacggatg cggtggcgga gttcgtggcg
      301 ggatgtgaga cggagggggt gcgggcgcgg cgtctgccgg tggactatgc ctcgcactcg
      361 gcgcatgtgg agacgctggc agccgagctg gaagtgatcc tggccgacgt ccgtccccgg
      421 gccggcgaga tcccgttcta ctcgacggtg gaagccgcgg cgatcgacac ggcgtcgttg
      481 gatgcggggt actggttcgg gaatctgcgt cggccggtgc ggttccagga gacggtcgag
      541 cagctgctcg cggacggttt cccggtgttc gtggagtgtg gtgcgcaccc ggtgctgaca
      601 gctgcggtcc aggagacggc ggagtccgtg ggccggcaag tgtgcgcggt cggttccctg
      661 cgccgcgagg agggaggtct gcgtcgtttc ctgacctcgg tggtggaagc cttcgtccag
      721 ggcgtcgacg tcacctggcc cgccctcttc gacgacaccg gtgcgcgtct cgtcgacctg
      781 cccacctacc ccttccagca ccagcgttac tggcttgagt cgcgtccttc tgcggcggtt
      841 gttccgtcgg ggacgcagga cggtctgtcg tacgaggtga cgtggaagag tctgtcgttg
      901 ccggagtcgg tgcgtcttga cggccgatgg ctgctcgtcg tacccgagga cctggacgcg
      961 gacggcacac gcatcgccca cgacgtcgag caagccctca ccacccacgg cgccaccgtg
     1021 cacacccttg ctcttgaccc cgccacagcg gacttcgacg gccgtttcag caggatgttc
     1081 gaggaagcaa cggacgtcac gggcatcctg tcactcctcg gactggctgt gggcccgcac
     1141 ccggagcacg gggaggtgcc gctcggggta gccacgtcgc tgacgctgac gcgccaagcc
     1201 ctggctgggt tcggtgttcc ggtgtgggtg gtgacgcggg gtgcggtgtc cgtcgtgcct
     1261 ggtgaggtgc cggagacggc gggtgcgcaa ctgtgggcgc tcggccgggt tgccgctctc
     1321 gaactccccg accgctgggg tggtctgatc gacctcccgg cggacacgga tgcgcggtcg
     1381 gtgggtctgg cggtgcgggc gctggccgcc gggatcgcgg ccggtgagga tcagctggcg
     1441 gtgcggccct cgggcgccta cggccggcgt ctcgtacgag ccaccgcccg ccggggacgg
     1501 aaggactggc gcccgcaggg cacggtgctg ctggccgggg acctcgatgc cgtcgctgag
     1561 ccgctggtcc gatggctgct cacggacggc gcggaccacg ttgtactcgc cgatcccgcc
     1621 ctgaccgaac tcccggccgc gctcgcggac gtggctcagc gcgtgacgac cgctgcggcg
     1681 cccgacctcg cggaccgtgc ggtcctcgcc gccctcgtca ccgagtacgc ccctgccatg
     1741 gtggtcgtcg ttccgcccgc ggtggagctc gctccgctcg agagcacgag cccggccgat
     1801 ctcgcggcgg ccgtcgccgc caagtccgct accgccgcgc acttcgacgc actgctcgac
     1861 gggccacacg caccggaact ggtactgatc tcctcggtcg cggggatctg gggtggtgtc
     1921 cggcagggcg cgtacgccgt cggcgccgct cacctcgatg ccctggccgc ccgccgccgg
     1981 gcccggggtc tgccgacggt ctccgtcgcg tggacgccct gggcgggttc cgttatcgcg
     2041 gacggctctg ccgccgagtc gatgcggcag tacggcatcg ctccgctgga gccccagacg
     2101 gcgctcgcgg agctgaaccg ggcgctgggc agcggcacgg gcgacgccgc ggtggccgac
     2161 gtcgactggg aacagttcct ggcgtccttc acctccgtac gtcccaccgt cctcttcgac
     2221 gagctgcccg aggtgcgccg tctccgtcag gcggaggcgg cggccatggc ggaccaggcc
     2281 gcccggatcg gtgcccccgg cggcacagag ctggcgcgct ccctgcgcga caagccccgg
     2341 aacgcccagc gaaaggccct cctggagctg gtcactgccc atgtggcggc cgtactgggt
     2401 gagggcgctc ccgacgcgat cgaccagagt cgggcgttca aggacatcgg cttcacctcc
     2461 atgaccgcga tggaactgcg caaccggctc aaggaggcca ccgggctcac ccttccggcc
     2521 tccctcgtct tcgaccaccc ccatccccgc gcactcgccg accatctgcg cgaggaacta
     2581 ctgggcgagg acgatgcgga acgcaccgac tccgagccga acaccccgag cggtccgcct
     2641 ccggcgggcc aggacgagcc gatcgccatc atcggcatgg cctgccgcct ccccggtgac
     2701 gtcgggacac ccgacgagct ctgggagctg ctcgaaaccg gccgcgacgc cattacggac
     2761 ctgcccgtca accgggggtg ggacgtggcg ggcctgtacg acccggatcc ggacgcggcg
     2821 gggcgcgcct acgtccgtga gggcggcttc ctccacgacg cgggggagtt cgacgcgggg
     2881 ttcttcggca tctcgccgcg tgaggccctg gcgatggacc cacaacagcg aatcatcctg
     2941 gaactcgcct gggaatcgtt cgaacgtgcg ggcctggacc cggccggccg acgcggcagc
     3001 cgtaccggcg tgttcatggg aaccaacggc cagcactaca tgccgctgct gcagaacggc
     3061 agcgagagct tcgacggcta cctcggcacc ggcaactcgg ccagcgtcat gtcgggccgc
     3121 atctcgtaca ccctcggcct ggaggggccg gcgctgacgg tggacacggc gtgttcgtcg
     3181 tcgctggtcg cgctgcatct ggcggtgcgg gcgctgcgca gtggtgagtg cgacctcgcc
     3241 ctcgcgggcg gtgcgacagt gatgtcgacg ccggaggtgc tcgtggagtt ctcccggcag
     3301 cgagcagtcg ccgccgacgg ccgctgcaag gcgttctccg cctccgccga cggcttcgga
     3361 ccggctgagg gtgcgggcgt actgctcgtc gagcgcttgt cggatgcggt gcgtcatggg
     3421 cgtcgggttt tggcggtggt gcgtggttcg gcggtgaatc aggacggtgc gagcaatg
//
