LOCUS       NZ_BMVE01000028        16328 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces goshikiensis strain JCM 4640 sequence28, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVE01000028
VERSION     NZ_BMVE01000028.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces goshikiensis
  ORGANISM  Streptomyces goshikiensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 16575)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650555.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:30:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,491
            CDSs (total)                      :: 7,410
            Genes (coding)                    :: 7,286
            CDSs (with protein)               :: 7,286
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 44 of 125
            Pseudo Genes (incomplete)         :: 101 of 125
            Pseudo Genes (internal stop)      :: 9 of 125
            Pseudo Genes (multiple problems)  :: 27 of 125
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:38:35
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 247
            Orig. end                         :: 16575
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVE01000028.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..16328
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="thioamitides"
                     /region_number="1"
                     /rules="((YcaO or TIGR03604) and TfuA)"
                     /tool="antismash"
     cand_cluster    1..16328
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((YcaO or TIGR03604) and TfuA)"
                     /kind="single"
                     /product="thioamitides"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..16328
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[10000:12377](-)"
                     /cutoff="20000"
                     /detection_rule="((YcaO or TIGR03604) and TfuA)"
                     /neighbourhood="10000"
                     /product="thioamitides"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12377)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="((YcaO or TIGR03604) and TfuA)"
                     /neighbourhood="10000"
                     /product="thioamitides"
                     /protocluster_number="1"
     gene            330..458
                     /locus_tag="IE248_RS38360"
                     /old_locus_tag="GCM10010336_75150"
     CDS             330..458
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE248_RS38360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010336_75150"
                     /product="hypothetical protein"
                     /protein_id="WP_268254123.1"
                     /transl_table=11
                     /translation="MSIPTTGYVSVRAENPDPAAYTAVRTVTTSTFTEVFGVDVAS"
     gene            493..699
                     /locus_tag="IE248_RS36730"
                     /old_locus_tag="GCM10010336_75160"
     CDS             493..699
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE248_RS36730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010336_75160"
                     /product="hypothetical protein"
                     /protein_id="WP_190033109.1"
                     /transl_table=11
                     /translation="MVDVRQAAIGGSALPPSSETGTARVVLRGDADDREQVATLLENSL
                     HCTREDAFQDDQTILDIALSQAH"
     gene            718..951
                     /locus_tag="IE248_RS38685"
                     /old_locus_tag="GCM10010336_75170"
     CDS             718..951
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF043066.1"
                     /locus_tag="IE248_RS38685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_75170"
                     /product="ETEC_3214 domain-containing protein"
                     /protein_id="WP_350878762.1"
                     /transl_table=11
                     /translation="MVTWSHEDDEVLMYSLTRHSRLFRPRVAVGPHRLTLGKTPLANLP
                     TPETDAAGPWPAKGGRRFGYAEQHYFGNRWLP"
     gene            959..1183
                     /locus_tag="IE248_RS36735"
                     /old_locus_tag="GCM10010336_75180"
     CDS             959..1183
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE248_RS36735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010336_75180"
                     /product="hypothetical protein"
                     /protein_id="WP_190033110.1"
                     /transl_table=11
                     /translation="MGVSDAGCSALPPLGCDGDGWSPEELESCRRQAPINSLLIAGSTI
                     DLDSFMPYGIAPDCDPAGSSTFGTPRALR"
     gene            complement(1290..3236)
                     /locus_tag="IE248_RS36740"
                     /old_locus_tag="GCM10010336_75190"
     CDS             complement(1290..3236)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008741971.1"
                     /locus_tag="IE248_RS36740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_75190"
                     /product="DUF3732 domain-containing protein"
                     /protein_id="WP_190033111.1"
                     /transl_table=11
                     /translation="MTFQIKAITIYGKQPGQIRTVPFKTGTLNIVTGDSRRGKSALLTI
                     IDYCLASTDYPVKAGKVRDYVSTYAVTLTKPGQQLFVARRAPEAKAAVSTVLCVITQAP
                     DTPPPQLEELNFTTPQDTAKDILSNFCGIDSTVRVPAVGKNTLIAPSIRHALFFCFQAQ
                     NEVANPDVLFHSQGEDWKPNTIRGVIPYFLGAVDREQALLHNRLRNLRRDLADNEAMLA
                     ASTDVTPASGQAQALLAEAVEVGLVPPRTGEQPADEVLRHLRSAMVHGAPQAGSPGDDP
                     IAALHTQRTELRRLFAQTRAKIADLKTDLAENNEFNDQAIEQRARLASLGLLNRDPDTA
                     DRARCPVCDSSVPAANETVVGLMRDLAHLDGDLQAIGSDTPAIRRLIATGEDRLQTLRS
                     ELARNQEEINELTTALRTLHREPDDLRRAAIVQGRISLFLDTAAQQVAAPRVHDRREAL
                     REQIAGLEEQLSGSTQEERLTSFLSLINQEIKKKAKALDLDFSGSPIRLDIGRLTVVAD
                     TVDGPVQLKNMGSAENHLGYHVSTLLSLHEWFAEHHGPVPRVLILDQPSQVYFPAEATG
                     EVELQTNDRMHLLNLFEALHHTLQQLDGKMQIIVMEHADLADPGFSQHVVERWRYGNDS
                     ALVPSAWMDHEDS"
     gene            complement(3233..3709)
                     /locus_tag="IE248_RS36745"
                     /old_locus_tag="GCM10010336_75200"
     CDS             complement(3233..3709)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039805.4"
                     /locus_tag="IE248_RS36745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_75200"
                     /product="three component ABC system middle component"
                     /protein_id="WP_190033112.1"
                     /transl_table=11
                     /translation="MDLSREERALYNPAFTALLCTRAVQGHEKQYDAACPLPVAVVAAV
                     MALQPSIRDVLPGTTKTGLMKWLDTNEAVRIAMGRNAAPLAAVVRPGLLFALQTGSLRA
                     TGEGLSLAAGATTKTIKGATQQTIAIQNAALLLGRWLPSTGSLSTVLTLLGVKP"
     gene            complement(3709..4854)
                     /locus_tag="IE248_RS36750"
                     /old_locus_tag="GCM10010336_75210"
     CDS             complement(3709..4854)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF042658.3"
                     /locus_tag="IE248_RS36750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_75210"
                     /product="ABC-three component system protein"
                     /protein_id="WP_190033113.1"
                     /transl_table=11
                     /translation="MCIEQWMSAGADWSVAVEAADDIEQHVGSVTELVQLKQRAEGVRL
                     TNLSKDLWKTLRIWAEAAGAGRIDLDSTNLYLLTTAELPKDSVGFLLQHRDSGHRNEEQ
                     ALKLLCAAAKASKSESKAMTESFAAFMGLANREVPLQRALLARIEVIGGADDIDVVRSS
                     MLGLAAVAAGREAAKSFLHRLEGWFYDRVIEQMRTPGGSPVTGVEFDEVFSGLQRQFSY
                     DNLPIDPDITGMTGAVDEETDKIFVRQLGLIGVGSERIGYAVRDYVRAFAQRSRWLNEN
                     LLRPGEIGEYERRLVEEWQSRFAEMAEELGPEATEAEKKKEARRIYRWVDQEARSQIRT
                     GCDEVFVPKGSYHMLADERRVGWHPDFAARLMQLLEPAEVR"
     gene            4994..5338
                     /locus_tag="IE248_RS36755"
                     /old_locus_tag="GCM10010336_75220"
     CDS             4994..5338
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE248_RS36755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010336_75220"
                     /product="hypothetical protein"
                     /protein_id="WP_190033114.1"
                     /transl_table=11
                     /translation="MSGILESRCSGQTHRVLVGVSPHLGAEHLDGVEGFWGMNDESVIT
                     IEIRGHEKAVLKAAQRISDLFLSSGPCRLRRTPGKDEVQVLVHADVDRGAGEGGYLDPG
                     EAEGAGAFLR"
     gene            5415..6116
                     /locus_tag="IE248_RS36760"
                     /old_locus_tag="GCM10010336_75230"
     CDS             5415..6116
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE248_RS36760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010336_75230"
                     /product="hypothetical protein"
                     /protein_id="WP_308438213.1"
                     /transl_table=11
                     /translation="MIKNLRRGLLATALAFAVLAPASPAQADEVTLMDGITALPVAEEI
                     REGYKRELYPHWKDDDKNGCTARNDVLIAEAIEAPTIGAGCTLTGGVWHSYYDDVLVQG
                     PSGVDIDHLIPLAEVHDSGGYGWTTERRRRYANDLGSDVTLVGVSARSNGRSPTRTRPP
                     GCPCPRSTAATSGNGSAPSTAGASPSTSPSGKPSCGTRPPALTPASTTTSRRDSAKSGS
                     WRLADAVLIFE"
     gene            complement(6862..7719)
                     /locus_tag="IE248_RS36765"
                     /old_locus_tag="GCM10010336_75250"
     CDS             complement(6862..7719)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006134989.1"
                     /locus_tag="IE248_RS36765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_75250"
                     /product="hypothetical protein"
                     /protein_id="WP_190033115.1"
                     /transl_table=11
                     /translation="MSWFFDALSSGFQHPTDPGHLQQFRIVFGTVLALRFALSIGQGGW
                     DRLAPGSLSLHLTQKRFGLTRTRLLAGAYRPMLIIRTAAALTLAAGLASRASLLLVLAG
                     AAMEMLYLKSPNSIHYTLLTGAALLAAGDLGHGLHIGHAPSTANTWAQCLLVLITTDIY
                     WNSAWQKTRSRQFRTGLYLAQWIHTYTQVKDQLPYRHEHAIPGFVRRHLGNLTNRDIRL
                     WRLLATTVIAAEIALPAALLFPRTTPYAAAAGIAMHIAFTCLKPRQLVTFSGLTTGTYF
                     AFAA"
     gene            complement(7703..8062)
                     /locus_tag="IE248_RS36770"
                     /old_locus_tag="GCM10010336_75260"
     CDS             complement(7703..8062)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527695.1"
                     /locus_tag="IE248_RS36770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_75260"
                     /product="hypothetical protein"
                     /protein_id="WP_190033116.1"
                     /transl_table=11
                     /translation="MPSSVLSAAQIAVTVALVAWMISGFTRLPGRWLRWKMFCRGNFII
                     VTLTGTTKDGRTEPVSAYSYLTPGSFLLGPPQLVAIVNHLVRSGQYDRIDGHGRLLSER
                     GEQRVEVRDSRVVVL"
     gene            complement(8128..9141)
                     /locus_tag="IE248_RS36775"
                     /old_locus_tag="GCM10010336_75270"
     CDS             complement(8128..9141)
                     /EC_number="1.1.1.94"
                     /GO_function="GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /GO_function="GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /GO_process="GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355798.1"
                     /locus_tag="IE248_RS36775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_75270"
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="WP_190033117.1"
                     /transl_table=11
                     /translation="MTQQRSLRAAVLSAGSWGTAVAKIMADAGTDVIVHARRSEIADTI
                     NTEHRNPGYFPDIELPFSVKATTDPAVALDGADFLVLSIPAQSLRASLAAWAPHIGPDT
                     VIVSLMKGIEAGSGLRASQVITDVTGVPDERVAVLSGPNLAAEIMAEQPAAATIACPDE
                     SAARRFQTACHTPYFRPYTSTDVIGCELGGAVKNVIALAVGIASGMGLGDNSTSLLITR
                     GLAEATRLATAMGAEPATLAGLAGVGDLVATCSSPLSRNRTFGTHLGRGLSVAEATAAT
                     RQTTEGVKSAEAILAMARTHGVEMPITDVMADLLNEKITLDEAAAALMQRPAKPER"
     gene            complement(9138..10004)
                     /locus_tag="IE248_RS36780"
                     /old_locus_tag="GCM10010336_75280"
     CDS             complement(9138..10004)
                     /EC_number="2.1.1.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006134986.1"
                     /locus_tag="IE248_RS36780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_75280"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_190033118.1"
                     /transl_table=11
                     /translation="MNTTTAAASGGRSAQYDAFHTARARTDLVARLYATAMGEDYPAET
                     AASSSCDWPLLGLMTARLRMRPGQLLVDAGCGTGGIGLWLARALALRLEGFDLSPVAVA
                     QAFGRRTHFLGSADRAAFRVAELEHTNLPDGHAHGIVCVDALGTATDRTAAVRELGRVL
                     SPGGRMLLTRSTRRGAEPAWPEQAHVAGLMLEHVDERPAEPAMWDRLYKLWIAHADDLR
                     REVGEVQTERMLREAHQMLPKLPSRRAVLLTLRRPAHDPAASGTADTMTAPRLHSGQPP
                     FHEEIPQ"
     gene            complement(10001..11146)
                     /locus_tag="IE248_RS36785"
                     /old_locus_tag="GCM10010336_75290"
     CDS             complement(10001..11146)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thioamitides: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018522479.1"
                     /locus_tag="IE248_RS36785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_75290"
                     /product="YcaO-like family protein"
                     /protein_id="WP_190033119.1"
                     /sec_met_domain="YcaO (E-value: 1.4e-60, bitscore: 196.1,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRADDLVVLPGTVRARTPEETWAAVAGLLPEFGITRLADLTGLDC
                     IGLPVWTAIRPASLTLSASQGKGATGLLAKLSAVMEAIELWHLEQPLPIAARGPAAEVA
                     PHCPVAALPMTVPYPEHTLRQMVWEWTQGTGLVSGHDELLPADLVRCRARRPEWSPDLL
                     RATSTGLACGNTPDEALLHALYEVVERDVLYREEADGRSWRTFIAPGTVEDPHAREVLD
                     KLAAAGMSLEIAMVDGPYGLPVCVAYLWSEDLPVVFAGGGCHSVPAIALTRALTEAAQS
                     RLTAISGTRDDLTSAPSSTGTPAFRPAQTSGLVPWPDATGHFTRADGGFADQAADLAVE
                     IAHATGHEPIALDLSHPNSPVHAVKIVCPGTRSRIRRSMPR"
     gene            complement(11136..12377)
                     /locus_tag="IE248_RS36790"
                     /old_locus_tag="GCM10010336_75300"
     CDS             complement(11136..12377)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     thioamitides: TfuA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700245.1"
                     /locus_tag="IE248_RS36790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_75300"
                     /product="TfuA domain-containing protein"
                     /protein_id="WP_229895805.1"
                     /sec_met_domain="TfuA (E-value: 3.9e-43, bitscore: 136.5,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFVGPTLAKTEPQLAVPGVRVCPPARHGDLFDGALHAGDTVVLVD
                     GVYHQALALRHKEILAAMGRGISMIGAASIGALRAAELTPFGMLGVGTIYTSYVRGEID
                     GDDEVAVGQAPDGQWDALTWPVVNLRHVLHLAQAAHVLKQDRAAHLLDALRAVYYPQRT
                     AAAVWAVCRRQGETDFAAWLAGRLDQNRHFGDLKRADALTALRTALTGWAQPAETRPAP
                     AVWETTYFCRWSNTFARTTVDGLELATEDRLVYQQIFDPHFRERWTAYLEHRSLNPADG
                     PVLPLDVRLAQLTGGDVPAHQVFHPPLDLRDEASVALLMEGETEQDRQAVAQYAVALAR
                     IHRSRPGFSTDAVRDDLTRQILLRVWQCTEADFDAEASARGLGCGARAVEAAKRIVPGF
                     LDESNERAEFGHAC"
     gene            complement(12749..13012)
                     /locus_tag="IE248_RS36795"
                     /old_locus_tag="GCM10010336_75320"
     CDS             complement(12749..13012)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740611.1"
                     /locus_tag="IE248_RS36795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_75320"
                     /product="hypothetical protein"
                     /protein_id="WP_229895806.1"
                     /transl_table=11
                     /translation="MSRPASKDPRPLRSAATRRAMAHRQLPRWLREGAVAVEDGGRRGV
                     VQFIGEWEDPATRRINPCAIFLRPEGGGVEWIVADHVTLTQG"
     gene            complement(13009..13737)
                     /locus_tag="IE248_RS36800"
     CDS             complement(13009..13737)
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /GO_function="GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA]"
                     /GO_process="GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025033.5"
                     /locus_tag="IE248_RS36800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="WP_308438214.1"
                     /transl_table=11
                     /translation="MSCGTSSLLPAGEPTVLSTAHPLQCDSHPKDHAMSRPASPPHVPG
                     TISRIPGPWDLNMIAMHGPITAAEQLFVAEHMVTGGAGRTAVEVGCGTGRFAHWLHHAH
                     GYHVTGLDLPPHNLRLARLRGQGTGLSYAAHDAETGPPPRTPRRRSGPCDSPHDHPLPA
                     RPSTAAAPGPRPVAPPRRLPLPPSAGEAVPRRTPGLGGAREPACLCGRLGQPTAHGRRP
                     PRPPSCSARPPAEPATKDTA"
     gene            13827..15122
                     /locus_tag="IE248_RS36805"
                     /old_locus_tag="GCM10010336_75340"
     CDS             13827..15122
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264467.1"
                     /locus_tag="IE248_RS36805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_75340"
                     /product="XRE family transcriptional regulator"
                     /protein_id="WP_190033121.1"
                     /transl_table=11
                     /translation="MSRPGNSKFADARRRLGWHSQSDVADAYETHSALLGEPATITVRQ
                     VRRWESADPGWPKRKMTRRVLESMFGVPLAELGFIPPAAGKDSVDQEEESMRRRSFLGG
                     FVVGAAPLLNPTGQGRLTAALANARRYSDHALVGHLRSALDEAARSDGRTGPRQALPTA
                     LDLLAVIDELARGTRSSVRRELLMVGARAAEFTAFLHRDAGAPPQVTLYFHDRAIEWAS
                     ITGDGPMHAYVLLRKAQATDRGDAARMLDLAHAAAQGSWRLPPRARAEALQQEARALAL
                     TGAPPRSVARTLGQAHEALAAADPAEPATCTGPLGAGYSENRLMAQSAICHREAGQPQQ
                     AVTLLRQHLAEQGAFATRDRAFFTAHLAGALAAAGQPDDAAATGLSALQLAAVPGFGQA
                     LGELRRTAGELRPYARRPAVRELRRALTALPV"
     gene            complement(15119..15934)
                     /locus_tag="IE248_RS36810"
                     /old_locus_tag="GCM10010336_75350"
     CDS             complement(15119..15934)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (32-167). E-value: 3e-12.
                     Score: 38.6. Matches aSDomain:
                     nrpspksdomains_IE248_RS36810_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1036: alpha/beta hydrolase fold protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264468.1"
                     /locus_tag="IE248_RS36810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_75350"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_190033122.1"
                     /sec_met_domain="Abhydrolase_6 (E-value: 8.3e-17, bitscore:
                     52.9, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 1.4e-11, bitscore: 34.9,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSELNVAFRSLDGTALKGTVTVPERMTAGVAVLVHGAGVTRQEG
                     GFFTRLAAGLAREGVRCLRFDLRAHGASGGRAEEVTIAGVANDIRAASDHLRAEAGVDR
                     PVHVIAASFSGGAAALHAAYRPQDVGRLVLLNPRLDYRDRYVTSRPYFDADYITASAAR
                     DLDERGYTEKSPWELGRALHNEVFYLDPDVYLRQVRAPALLVHGTKDTFVPVESSRRYL
                     PVFGGPAELMELDGAQHGFAVHADPQYADPQTQVWQAAVIEKVCGFLGG"
     aSDomain        complement(15434..15838)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE248_RS36810_Abhydrolase_1.1"
                     /evalue="3.00E-12"
                     /label="IE248_RS36810_Abhydrolase_1.1"
                     /locus_tag="IE248_RS36810"
                     /protein_end="167"
                     /protein_start="32"
                     /score="38.6"
                     /tool="antismash"
                     /translation="VLVHGAGVTRQEGGFFTRLAAGLAREGVRCLRFDLRAHGASGGRA
                     EEVTIAGVANDIRAASDHLRAEAGVDRPVHVIAASFSGGAAALHAAYRPQDVGRLVLLN
                     PRLDYRDRYVTSRPYFDADYITASAARDLDE"
     misc_feature    complement(15907..15921)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 19.08"
                     /tool="antismash"
     gene            16129..>16328
                     /locus_tag="IE248_RS36815"
                     /pseudo=""
     CDS             16129..>16328
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE248_RS36815"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MEAPGRPFLMHIGDLFWQNQGRVVMLAGLIERGRVRKGDAVEIVG
                     FGGTALVAVEEIEASRRYVDE"
ORIGIN
        1 caccaccccc aaccccaccc cacccacaca caaagaagcg acgaccaccc ccgtctgaag
       61 ctccgccacc accgttcttt cctgtcgcac gcaacgtcgg cacataacca cttcacgacc
      121 acggcgggat ggtgcgggag agcacgagag ggcggcagcc ctctctctcg ccacacgacc
      181 tgtcacccga catcgctgcg ggatcccgac tccccgcgcc cagaagcaac gcctgcgggc
      241 taccgccctc gtggccacac cccattggcg cgacagatca gtcaacgggc cggatgcttg
      301 gatgcaccag ccgtgacgag gaggccgtga tgtcgattcc cactaccggc tacgtgagtg
      361 tgcgagcgga gaatcccgat cccgccgcgt acaccgccgt cagaacggtc accacctcca
      421 ccttcacgga ggtgttcggc gtcgatgtcg catcgtaacg agccaccagg ttcggctacg
      481 cagcacggct tcatggtcga tgtaaggcag gccgccattg gcggcagcgc cctgccccca
      541 tcgtccgaga cgggcaccgc acgagtcgtc ctgcgtggtg acgccgacga ccgggaacag
      601 gtcgccaccc tgcttgagaa ctcgctccac tgcacgaggg aggacgcctt tcaggacgac
      661 cagaccatcc tggacatcgc cctctcccag gcgcactgag cggaagaact cgggttcctg
      721 gtcacgtggt cgcacgagga cgacgaggtg ctgatgtact ccctcacgag gcacagccga
      781 ctcttccgcc cgcgcgtcgc tgttgggcct caccggctca ccttgggcaa gacccccctt
      841 gccaatctgc ccacccccga aacggatgcc gccggcccgt ggccggccaa gggagggcgc
      901 cgcttcggct acgccgagca gcactacttc gggaaccggt ggctaccttg attgggtcgt
      961 gggagtgagc gacgcgggct gctcagcgct tcctcccctg gggtgcgacg gggacgggtg
     1021 gagcccggag gagctggaat cttgccggcg ccaggcgccc atcaactcgc tgctcatcgc
     1081 aggttccacg atcgacctgg acagcttcat gccgtacggc atcgctccgg actgcgaccc
     1141 ggccggctcg tcgaccttcg gcaccccgcg cgcgctgcgc tagccaccgg gttcgagaac
     1201 atggggaggc gcgtgcaacg tctgcgagac cgtcgcaggc gctccgtctc ggcggttcct
     1261 gagtagtcag ttgtgctgac gagccgctct caactgtcct cgtggtccat ccatgcggaa
     1321 ggcaccaggg ctgagtcgtt gccgtagcgc cagcgctcga ctacgtgctg gctgaagccg
     1381 gggtcggcga ggtcggcatg ttccatgacg atgatctgca tcttgccgtc gagctgctga
     1441 agggtgtggt ggagggcttc gaagaggttc agcaggtgca tgcggtcgtt ggtttggagc
     1501 tccacttctc cggtggcttc agccgggaag tagacctgcg agggctgatc caaaatcagg
     1561 acacggggca caggcccgtg gtgctcggcg aaccactcat ggaggctgag cagggtggag
     1621 acgtgatagc ccaggtgatt ctcagcgctg cccatgttct ttagctgcac agggccgtcg
     1681 acggtgtcag cgaccacggt gagccggccg atgtccaagc ggatgggcga gccagagaag
     1741 tcgaggtcga gtgccttcgc cttcttcttg atctcctggt tgatcagcga caggaagctg
     1801 gtcagtcgct cctcctgggt actcccgctg agctgctcct caaggccagc gatctgctct
     1861 cgcagcgcct cacggcgatc gtgaacccga ggtgcggcaa cttgctgggc agctgtgtcg
     1921 aggaagagac tgatgcggcc ctggacgatg gcggctcgtc tcaagtcgtc tggctctcgg
     1981 tggagcgtgc gcagtgctgt ggtgagttcg ttgatctcct cctggttgcg ggcgagctcg
     2041 gagcgcagtg tctgcagacg gtcttcgcca gtcgcgatga ggcgccggat ggctggggtg
     2101 tcgcttccga tcgcttgcag atcgccgtcc aggtgggcga ggtcccgcat gaggccgacg
     2161 acggtctcgt tggccgcggg gacgctgctg tcgcagaccg ggcagcgtgc tcggtctgcg
     2221 gtgtcggggt ctcggttcaa gaggcctagg gacgccaggc gggctcgctg ctcgatggct
     2281 tgatcgttga attcattgtt ctctgccaga tccgtcttca agtcggcgat cttcgcccgg
     2341 gtctgcgcga agagccgacg tagttcagtt cgctgggtgt ggagagcggc gatggggtca
     2401 tcgccgggcg agcctgcctg cggggcgccg tggaccatgg cgctgcgtag gtgccggagg
     2461 acttcgtctg ccggttgctc gccggttcgt ggaggcacga ggccgacctc gacggcttcg
     2521 gccagcaggg cctgggcctg cccgctcgcc ggggtgacgt cagtggacgc agcgagcatg
     2581 gcttcgttgt cggcgaggtc ccggcgcagg ttgcggagcc ggttgtggag cagagcctgc
     2641 tcgcggtcga ccgcgcccag gaagtatggg atgacacccc ggatggtgtt cggcttccag
     2701 tcttcgccct gcgagtggaa taggacgtca gggttggcaa cctcgttctg ggcctggaag
     2761 cagaagaaga gggcgtggcg gatggacggc gctatgagcg tgttcttgcc gacggcaggt
     2821 acccggacgg tgctgtcgat gccgcagaag ttgctgagga tgtccttggc tgtgtcctgc
     2881 ggggtggtga agttcagctc ttccagctgc gggggcgggg tgtcaggcgc ctgggtgatg
     2941 acgcacagca cggtgctgac tgccgctttg gcttcagggg cccggcgggc aacgaagagt
     3001 tgttggccgg gtttggtgag ggtgacggcg taggtgctga cgtagtcgcg gaccttgcct
     3061 gctttgactg ggtagtcggt gctggcgagg cagtagtcaa tgatcgtcag cagtgcgctt
     3121 ttgccgcgcc gagagtcgcc cgtaacgatg ttgagggtgc ctgtcttgaa gggaacggtg
     3181 cggatctgtc cgggctgctt gccgtaaatg gtgatcgctt tgatctggaa cgtcacggtt
     3241 tgactccgag aagcgtcagg acggtgctga ggctgcccgt gctgggcagc cagcgcccga
     3301 ggagcagggc ggcattttgg atggcgatgg tctgctgagt ggcgcccttg atggtcttgg
     3361 tcgtcgcgcc agcagccaag ctgagccctt ctcctgtagc ccgcagggat ccggtctgga
     3421 gggcgaagag cagcccggga cggaccaccg ccgcgagcgg ggcggcgttg cggcccatgg
     3481 cgatgcgcac ggcctcgttc gtgtcgagcc acttcatcag cccggtcttc gtcgtcccgg
     3541 ggaggacgtc cctgatggaa ggctgaaggg ccatcaccgc ggccacgacg gccacgggca
     3601 aggggcacgc agcgtcgtac tgcttctcgt gaccctgaac tgcgcgggtg cacagcaacg
     3661 cggtgaaggc ggggttgtac agagctcgct cctcacgact caggtccatc agcggacctc
     3721 ggccggctca agaagctgca tcagccgggc cgcgaaatcc gggtgccaac cgacccggcg
     3781 ctcgtcggcc agcatgtggt acgagccctt gggcacgaac acctcatcgc atccggtgcg
     3841 gatctgagag cgggcttcct ggtcgaccca ccggtagatc ctcctggctt ccttcttctt
     3901 ctccgcctcg gtggcctcgg gtcccagttc ttccgccatc tccgcgaagc gcgactgcca
     3961 ctcctccacg agccggcgct cgtactcacc gatctcgccg ggacgcagca ggttctcgtt
     4021 caaccagcgg gaacgctgcg cgaaggctcg gacgtagtcc ctcaccgcgt agccgatacg
     4081 ctccgagccc acaccgatca acccgagctg ccgaacgaag atcttgtcgg tctcctcgtc
     4141 gacagcgccg gtcatgccgg tgatgtccgg gtcgatcggc aggttgtcgt agctgaactg
     4201 tcgttggagt ccgctgaaaa cctcgtcgaa ttcgacgccg gtgactgggc tgccgcccgg
     4261 cgtgcgcatc tgctcgatca cgcggtcgta gaaccagccc tccagcctat ggagaaagga
     4321 cttggctgcc tcgcggcctg cggccaccgc tgcgaggccg agcatggacg agcgcacgac
     4381 gtcgatgtcg tcggcacccc cgatgacctc gatccgcgcg agcagcgcac gctggagtgg
     4441 cacctcgcgg ttggccagtc ccatgaatgc ggcgaacgac tccgtcatcg ccttgctctc
     4501 gctcttcgag gccttcgccg cggcgcagag tagcttcagc gcctgctcct cgttgcggtg
     4561 accgctgtca cgatgctgaa gcaggaagcc cacgctgtcc ttgggaagct cggccgtggt
     4621 gagcaggtac agattcgtgc tgtcgaggtc gattcgaccc gccccggcag cttccgccca
     4681 gatccgcagg gtcttccaca aatccttcga aaggttcgtc aaacgcaccc cttcggcgcg
     4741 ctgcttcagc tgcacaagct ccgtcacgga gcccacgtgc tgctcaatgt cgtcggccgc
     4801 ctcgaccgca acgctccagt ccgcgccagc gctcatccac tgctcgatac acatgtgcag
     4861 agccttggcg aactggaaca gatacccgag agccgacgct gacgcctcgt actgttctcc
     4921 cccgtctgcc atcacacccc ccaagccaga ccggttcagc gatcattcca cgagtacgac
     4981 gatccgcata caggtgagcg gcatactcga atcgcgctgt tcggggcaaa cgcaccgggt
     5041 gctcgttggt gtgagtcctc acctgggtgc ggaacacctc gatggtgtcg agggtttctg
     5101 gggcatgaac gacgagagcg tgatcacgat cgagatccgg ggccacgaga aggccgtgct
     5161 caaagcggcg cagcgaatct cggacctgtt cctgtctagc ggtccgtgcc ggctccgacg
     5221 tacgcccggc aaggacgaag tgcaagttct cgtccacgcg gacgtcgatc gtggggccgg
     5281 tgagggcggc tacctcgacc ctggcgaggc ggagggcgca ggcgcgtttc tgcggtgaac
     5341 cggctcaccc agcggggtga gccgtcccca acccggctcg atccggcagt gggtgacccg
     5401 gtatcaccag gttcatgatc aagaacttgc gccggggact gctggccacc gccctcgcct
     5461 tcgccgtgct cgcccccgcc tcccccgccc aggccgacga agtcacgctc atggacggga
     5521 tcaccgcgct gcccgtcgcc gaggagatcc gcgagggcta caagcgggag ctgtacccgc
     5581 actggaagga cgacgacaag aacggctgca ccgcccgcaa cgacgtcctg atcgccgaag
     5641 ccatcgaagc ccccaccatc ggcgccggct gcaccctcac cggaggggtg tggcactcct
     5701 actacgacga cgtcctcgtc cagggcccgt ccggcgtcga catcgaccac ctcatcccgc
     5761 tcgccgaagt ccacgactcc ggcggctacg gctggaccac cgaacgccgc cgccgctacg
     5821 ccaacgacct cggctccgac gtcaccctcg tcggcgtctc cgcccgctcg aacggcagaa
     5881 gtccgaccag gacccggcca cctggatgcc catgcccgcg gtccactgcc gctacctcgg
     5941 ggaatgggtc ggcaccaagc accgctgggg cctcgccgtc gaccagcccg agcgggaagc
     6001 cctcctgcgg tacgcggcct cctgccctaa cgcccgcatc aactacgacc tcgcgacgtg
     6061 acagcgcaaa gtctggatcc tggaggctcg ctgacgccgt tttgatcttc gaatgaggcc
     6121 cgcaggggct ccatcgcggg tgagtgatgc ccggcttggg gcacgagcga aaggacgagg
     6181 cccgagaagg cacctgtacg gccctgtgac ggcctgaccc cccttcccgg ggtgatctga
     6241 ccgccggttt cagagttttt ctttcggtcc tcctccatca gtacatagcc cgatgtactg
     6301 ttggcacagc gaatagaccc ttcacagttg gttccagacg ctccagtaca tagcgtgaaa
     6361 tctttcgaga cttctgtcgg gacacaaaaa agtccgccac ccgtgggtgg cggacctccg
     6421 gaacggctgg ccaggggtgg ttgagtcacc cctggctggc taccggatac cgatgactgc
     6481 cgtcacaaga gtgacgcacg ccgtggccag ggccatcact tcagcccaca gccgccacct
     6541 ggggtccttg tcggaaccgc cgtcatcgga ttgcgtagcg ctcatgagga cgaacctctc
     6601 ctttcttcac gggcacacca ccgtgcgccc tcggccgaag gcagcggaaa ccccgatgcc
     6661 tcccccgagg agggcacgtc agcgaacccc tgataaaagg gcgcgtcagc aaaagaacac
     6721 tatcgatgcg gcgactcccc cgtgctgcaa accggaaatc acagccctgg ccacgttctt
     6781 gcagctgcag cggaacgccg tccagtatca gcgtcccgtt ggcttcttcg accagagcgt
     6841 ggtgccgctg cggggtggtt ctcaggctgc gaaggcgaag taagtgccgg tggtgaggcc
     6901 ggagaaagtg acgagctgcc ggggcttgag gcaggtgaag gcgatatgca tcgcgatgcc
     6961 cgcggcggcg gcgtacggcg tcgtcctcgg gaagagcagc gcggcgggta gcgcgatttc
     7021 cgctgcgatg acggtggttg ccagcagccg ccagaggcgg atatcgcggt tggtgaggtt
     7081 gcccaggtgg cggcggacga agccggggat tgcgtgctcg tgccggtagg gcagctggtc
     7141 tttgacctgg gtgtaggtgt ggatccattg ggctaggtag aggccggtgc ggaactgccg
     7201 cgagcgcgtc ttctgccagg cggagttcca gtagatgtcg gtggtgatca ggacgaggag
     7261 gcactgcgcc caggtgttgg cggtgctggg ggcgtgtccg atgtgaaggc cgtggccgag
     7321 gtctccggcg gccaggaggg cggctccggt cagcagggtg tagtggatcg agttggggct
     7381 cttgaggtag agcatttcca tggcggctcc ggccaggacc agcaggagtg aggccctcga
     7441 tgcgagaccg gcagccaggg tgagggcggc ggctgtgcgg atgatcagca tgggccggta
     7501 ggcgccggcg aggagacggg tccgggtgag gccgaagcgc ttctgggtca ggtggaggct
     7561 cagggagccg ggggcgagtc ggtcccagcc gccctggccg atggacaggg cgaaccgcag
     7621 ggcgaggacg gtgccgaaga cgatgcggaa ctgctggagg tgtccggggt cggtggggtg
     7681 ctggaagccg gaggagagag cgtcaaagaa ccacgacacg gctgtccctc acttccacac
     7741 gctgctcgcc ccgctcggat aggaggcggc cgtgcccgtc gatccggtcg tactggccgc
     7801 tgcggacgag atggttgacg atcgcgacga gctggggcgg acccaggagg aaggatccgg
     7861 gggtcaggta gctgtaggcg ctgaccggtt cggtgcggcc gtccttcgtg gtgccggtga
     7921 gggtgacgat gatgaagttg ccccggcaga acatcttcca gcgcagccag cggccgggca
     7981 gacgggtgaa tcccgagatc atccacgcca cgagggcgac ggtgacggcg atctgggcag
     8041 cggacagcac ggatgagggc atggcgaacc tccgggtcag gagggagaag gtgtcggtgg
     8101 gcggccccgc gggcggggcg ccgggggtca gcgctcgggc ttggcgggcc gctgcatcag
     8161 cgcggcggcg gcctcgtcga gcgtgatctt ctcgttcagc aggtcggcca tgacgtccgt
     8221 gatcggcatc tcgacgccgt gggtgcgggc catggcgagg atcgcctccg cgctcttgac
     8281 gccttcggtg gtctgccggg tcgcggctgt cgcctccgcg acgctcaggc cgcggccgag
     8341 gtgggtgccg aaggtgcggt tgcgcgagag cggggaggag caggtggcga ccaggtcgcc
     8401 cactccggcc aggcccgcga gggtggccgg ctcggcgccc atcgccgtgg ccaggcgggt
     8461 tgcctcggcc agcccgcggg tgatgagcag ggaggtcgag ttgtcgccga ggcccatgcc
     8521 cgaggcgatg ccgaccgcga gggcgatgac gttcttcacc gcgccgccga gctcgcagcc
     8581 gatgacgtcg gtgctggtgt agggccggaa gtacggggtg tggcaggccg tttggaagcg
     8641 gcgggcggcg gattcatcgg ggcaggcgat ggtggcggcg gcgggctgct ccgccatgat
     8701 ctcggctgcc aggttcgggc ccgacaggac cgcgactcgt tcatcgggta caccggtcac
     8761 gtcggtgatg acctggctcg cccgcagccc gctcccggcc tcgatgccct tcatcagcga
     8821 cacgatcacc gtgtcgggcc cgatgtgcgg cgcccacgcg gcaaggctgg cccgcaggga
     8881 ctgggcgggg atggacagca cgaggaagtc cgcgccgtcc agggctacgg ccgggtcggt
     8941 ggtggccttc acggagaagg gcagctcgat gtccgggaag tagccggggt tgcggtgctc
     9001 cgtgttgatc gtgtcggcga tctcgctgcg acgagcgtgc acgatgacgt cggtaccggc
     9061 gtcggccatg atcttggcga cggcagtgcc ccaggacccg gcggagagca ccgctgcgcg
     9121 gagggaacgc tgctgggtca ctgcgggatc tcctcgtgga acgggggctg gccgctgtga
     9181 aggcggggcg cggtcatcgt atcggccgtg ccggatgcgg cgggatcgtg tgcggggcgc
     9241 cgtagggtca gcaggacggc gcggcggctg ggaagtttgg gcagcatctg gtgcgcctcg
     9301 cggagcatcc gttcggtctg gacttcgccg acttcgcgcc gcaggtcgtc ggcatgggcg
     9361 atccacagct tgtagaggcg gtcccacatg gccggctcgg cggggcgctc gtcgacgtgc
     9421 tccagcatga gcccggccac gtgggcctgc tcgggccatg cgggttcggc gccgcgcctc
     9481 gtcgagcggg tcaggagcat tcggccgccc ggggacagga cgcggccgag ttcgcgcacc
     9541 gcggcggtcc ggtcggtggc ggtgccgaga gcgtccacgc acacgatgcc gtgggcatgg
     9601 ccgtcgggca ggttggtgtg ctccagctcg gctacgcgga aggcggcccg gtcggcggag
     9661 ccgaggaagt gggtgcggcg gccgaaggcc tgggcgacgg cgaccgggga gaggtcgaag
     9721 ccctccaggc gcagcgcgag ggcgcgggcc agccacaggc cgatgcctcc ggtcccgcat
     9781 ccggcgtcca cgaggagctg gccgggccgc atccgcaggc gtgcggtcat cagcccgagc
     9841 aggggccagt cgcaggagct ggaggccgcg gtctcggccg ggtagtcctc ccccatcgcc
     9901 gtggcgtaga ggcgggcgac gaggtcggtg cgggcgcggg cggtgtggaa ggcgtcgtac
     9961 tgggcggagc gcccgccgga cgccgcagca gtggtggtgt tcatcggggc atcgacctcc
    10021 tgatccggga gcgggtgccg gggcagacga tcttcacggc gtggaccggg ctgttcgggt
    10081 gggagaggtc cagggcgatg ggttcgtgcc cggtggcgtg ggcgatttcc acggccaggt
    10141 cggcggcctg gtcggcgaag ccgccgtcgg ctcgggtgaa gtggccggtc gcgtccggcc
    10201 acggtacgag cccggaggtc tgtgcggggc ggaaagccgg ggtacccgtg ctgctggggg
    10261 cgctggtgag gtcgtcgcgg gtcccggaga tcgcggtcag ccgggactgc gcggcttccg
    10321 tcagcgcccg ggtcagcgcg atcgccggaa cgctgtgaca gccgccgcct gcgaagacca
    10381 cggggaggtc ctccgaccac agatacgcca cgcacaccgg cagcccatac gggccgtcga
    10441 ccatcgcgat ctccagcgac atgcccgcgg ccgcgagctt gtcgagaacc tcacgagcat
    10501 gcgggtcctc gaccgtcccg ggcgcgatga aggtgcgcca gctcctgccg tccgcctcct
    10561 cccggtagag cacgtcacgc tccaccacct cgtacagggc atgcagcagt gcctcatccg
    10621 gtgtgttccc gcaggccagg cccgtgctgg tcgcgcgcag caggtccggg ctccactcgg
    10681 gcctgcgtgc ccggcatcgg acgaggtcgg ccgggagcag ttcgtcgtgt ccgctcacca
    10741 ggcccgtacc ctgggtccac tcccacacca tctggcgcag ggtgtgctcg gggtagggga
    10801 cggtcatcgg aagcgcggcc acggggcagt ggggggcgac ctcggcggcc gggccgcgtg
    10861 ccgcgatggg caggggctgc tcaaggtgcc acaactcgat ggcctccatg accgccgaca
    10921 gcttcgccag cagcccggtg gcgcccttgc cctgggaggc gctcagggtc agggaggcgg
    10981 gccggatcgc cgtccagacc ggtagcccga tgcagtccag gccggtgagg tcggcgaggc
    11041 gggtgatccc gaactcgggc agaagcccgg ccaccgccgc ccacgtctcc tcgggggtcc
    11101 gtgcgcgcac cgttcccggc agcaccacca ggtcgtcagc acgcatgacc gaactccgct
    11161 ctctcgttgc tctcgtctag gaagccgggg acgatccgct tggccgcctc aacggcgcgt
    11221 gccccgcagc cgagcccgcg cgcggacgcc tccgcatcga agtccgcctc cgtgcactgc
    11281 cacacccgca gcaggatctg gcgggtgagg tcgtcgcgca ccgcgtcggt ggagaagccg
    11341 ggccggctgc ggtgtatccg cgcgagggcg acggcatact gggcgaccgc ctggcggtcc
    11401 tgttccgtct cgccctccat cagcagggcc acgctcgctt cgtccctcag gtcgagcggc
    11461 gggtggaaga cctgatgggc gggcacgtcc ccgcccgtga gctgggccag gcgcacgtcg
    11521 agcggcagaa ccggcccgtc ggccgggttc agggagcggt gctcaaggta ggccgtccac
    11581 ctctcgcgga aatgcgggtc gaagatctgc tggtagacca agcggtcttc cgtggccaac
    11641 tccagcccgt caacggtggt gcgggcgaag gtgttggacc agcggcagaa gtacgtcgtc
    11701 tcccagacgg ccggtgcagg ccgggtctcg gctggctgcg cccatccggt cagcgcggtg
    11761 cgcagcgcgg taagggcgtc ggcgcgtttg aggtcgccga agtgccggtt ctggtcgagg
    11821 cgcccagcca gccacgcggc gaagtccgtc tctccttggc ggcggcacac ggcccacacc
    11881 gcggccgcgg tgcgctgcgg gtagtagacc gcgcgcaggg catccagcag gtgggcggcc
    11941 ctgtcttgtt tgagtacgtg ggcggcctgg gccaggtgca ggacgtgccg gaggttgacg
    12001 acgggccagg tcagcgcgtc ccactgcccg tccggggctt ggccgaccgc cacctcgtcg
    12061 tcgccgtcga tctctccgcg cacgtaggag gtgtagatgg tgccgacgcc gagcatcccg
    12121 aacggcgtga gctcggccgc ccgcagcgcg ccgatgctcg ccgcgccgat catggagatg
    12181 ccccggccca tggcggccag gatctccttg tggcgcaggg ccagcgcctg gtggtagacg
    12241 ccatcgacga ggaccaccgt gtcgccagcg tgcagggcgc cgtcgaagag gtcgccgtgc
    12301 cgggctggtg gacagacccg aacaccgggg acggcgagct gcggctcggt cttcgcgagc
    12361 gtcggcccga cgaacacgtg aatcaccttg ggacctccag ccggcaatgg tgtgaaaggt
    12421 gaggacgggc gcccctcccc cggccccgtg gggagacaga caggggaggg gcgcccggtt
    12481 cagggtgttc gaggggcggc tactcctcgt cgaagacgtc ctgggcgact accacggctt
    12541 cggcgatctc ttccatcgcc tcggccggcg tcgcggaact ggcggctgcg gacttggact
    12601 gctccacggg tttctccttc ttcatgtgtt gcggttgcgg acggtacggg cggccaacca
    12661 cccgtaccgg tggtggagcg ggccttgggg ctggtcgcct gcgggtccgc tcgcccgccc
    12721 cggaacgggg cggggggtct gggggtgttc agccctgggt gagggtgacg tggtctgcga
    12781 cgatccactc cactccgccg ccctcggggc gcaggaagat cgcgcacggg ttgatgcggc
    12841 gggtggcggg gtcctcccac tcgccgatga actggacgac gcctcgacgt ccgccgtctt
    12901 cgacggccac cgcgccctcg cggagccagc gagggagctg acggtgggcc atggcgcggc
    12961 gggtggccgc cgatcggaga ggtcgggggt ccttcgaggc ggggcggctc atgctgtgtc
    13021 cttggtggct ggctcagcgg ggggacgtgc ggagcacgag ggtgggcgcg ggggccttcg
    13081 tccatgcgct gtcggctggc ccagccgtcc acataggcag gcaggttctc gggcgccacc
    13141 cagccctgga gttcgtcggg gaacggcttc gccagcactt ggaggtagag gcagcctcct
    13201 gggcggagcc accggtcgcg gacctggcgc agcagctgta gagggtcgcg caggtagcgg
    13261 gtggtcatgc gggctatcac aaggtccact ccgtcgacgg ggagtccggg ggggcgggcc
    13321 cgtctcggcg tcgtgggccg cgtacgacag gccggtcccc tgcccgcgga gcctggcgag
    13381 gcgcaggttg tggggtggga ggtcgagtcc ggtcacgtgg tagccgtgcg cgtggtggag
    13441 ccagtgcgcg aagcgtccgg tgccgcagcc gacttcgacc gccgtacggc cggctccccc
    13501 ggtgaccatg tgctcggcga cgaacagctg ctccgcggcg gtgatcgggc cgtgcatcgc
    13561 gatcatgttc aggtcccagg gaccgggaat cctgctgatg gtgcccggca catgcggcgg
    13621 ggaggcggga cgactcatgg cgtggtcctt ggggtggctg tcgcactgga gtggatgggc
    13681 cgtactgagg accgtaggtt cgcccgcggg aagcaacgag gacgtaccac aggacatttg
    13741 acgggagtaa cgtcagagtt ctcactccga gcggccgcga tctcacggcc gccgcttctc
    13801 cgcgaggtcc gcgaccgggg gtagccatgt cccgaccggg aaacagcaag ttcgccgatg
    13861 ctcgtcggcg cctgggctgg cacagccaga gcgatgtcgc cgacgcctac gagacccact
    13921 cggcgctgct gggcgaaccc gcgaccatca ccgtgcgcca ggtgcgccgg tgggagtcgg
    13981 ctgaccccgg ctggcccaag cggaagatga cccggcgcgt cctggagtcg atgttcgggg
    14041 tgcctctcgc cgagctcggc ttcattcctc ccgccgcggg gaaggactcc gtcgaccagg
    14101 aggaggagtc catgcgccga cgttccttcc tcggcggctt cgtcgtgggc gcggcgccgc
    14161 tcctcaaccc caccggccaa ggccggctca ccgccgccct cgccaacgcc cgccgctaca
    14221 gcgaccacgc cctggtcggc catctgcgct ccgcgctgga cgaagccgcg cgcagcgatg
    14281 gacgcaccgg accgcgccag gcactgccca ccgcgctgga ccttctcgcc gtcatcgacg
    14341 aactcgcccg gggcacccgc tcgtccgtcc gccgcgagct actcatggtc ggcgcccgcg
    14401 ccgccgagtt caccgcgttc ctccaccgcg acgccggagc cccgccccag gtcaccctgt
    14461 acttccacga ccgggccatc gagtgggcca gcatcacggg cgacggcccg atgcacgcgt
    14521 acgtcctgct ccgcaaagcc caggccaccg atcgcggaga tgcggcccgc atgctcgacc
    14581 tcgcccacgc cgccgcccaa ggctcctgga gactgccacc gcgcgcccgc gccgaagccc
    14641 tccagcaaga agcccgcgct ctcgcactca ccggcgcccc gccccggtcc gtcgcccgca
    14701 ccctcggcca ggcccacgag gccctcgccg ccgcggaccc cgccgagccg gcgacctgca
    14761 ccggaccgct gggcgccgga tacagcgaga accggctcat ggcccagagc gcgatctgcc
    14821 accgcgaagc cggacagccc cagcaagcgg tcaccctgct ccgccagcac ctggcggagc
    14881 aaggtgcgtt cgccacccgc gaccgggcct tcttcaccgc ccatctcgcc ggggccctgg
    14941 ccgccgccgg acagcccgat gacgccgccg ccacgggact gtccgcactc cagctcgccg
    15001 cggtccccgg cttcggccag gcactcggcg aactacgccg caccgccggt gagctccgcc
    15061 cgtacgcacg acggcccgcc gtccgcgaac tccgccgggc cctcaccgcc ctgccggtct
    15121 aaccgccgag gaaaccgcag accttctcga tcacggcggc ctgccacacc tgggtctgcg
    15181 gatccgcgta ctgcgggtcc gcatggacgg cgaagccgtg ctgcgctccg tccagctcca
    15241 tcagctcggc cgggcccccg aacaccggga ggtagcggcg ggaggactcc accggcacga
    15301 acgtgtcctt cgtcccgtgt acgagcaggg ccggcgcccg cacctggcgc agatagacat
    15361 ccgggtcgag gtagaacacc tcgttgtgca gggcccggcc cagctcccac ggggacttct
    15421 ccgtgtaacc acgctcgtcc aggtcccggg cagcggacgc ggtgatgtag tcggcgtcga
    15481 agtacgggcg gctggtgacg taccgatccc gatagtccag acgcggattg agcagcacca
    15541 gacggccgac gtcctgtggc cggtaggccg cgtggagcgc tgcggcccct ccggagaacg
    15601 aggctgcgat cacatgcacc ggccggtcca cacctgcctc ggcacgcaga tgatcactcg
    15661 cggcccggat gtcgttggcg actcccgcga tcgtcacctc ctcggcccgg ccaccgctcg
    15721 cgccgtgcgc gcgcaggtca aaccggaggc accgcacacc ctcacgggcg agcccagcgg
    15781 cgagccgggt gaagaacccg ccctcctgcc gagtcacccc ggcaccgtgc acgaggacgg
    15841 ccacaccagc cgtcatccgt tccggcaccg tcaccgtccc ctttagcgcg gtgccgtcca
    15901 acgacctgaa cgcgacgttc aactcagagg tcatggacaa atcgtagaga ccacgttcgc
    15961 cggggacgag cgcgcagaaa gaatccgcgg ttgctcgtgg tcgagccgct gctcggtagg
    16021 cctagggcct gtccgatggg acgcagcacc gatcgggcac agggtgggca gtgatcgctg
    16081 ttaacctcgg cagttcgttg atcgctggtc gttgggaagg gagcccagat ggaagcaccg
    16141 ggcaggccgt tcctgatgca tatcggagat ctcttctggc agaaccaggg cagggtggtc
    16201 atgctggccg gcctgatcga gcgcgggcgg gttcgtaagg gtgatgcggt ggagatcgtc
    16261 ggtttcggtg ggaccgcgct cgtcgccgtc gaagagatcg aagcttctcg gcggtatgtc
    16321 gacgaggc
//
