LOCUS       NZ_BMVE01000016        10972 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces goshikiensis strain JCM 4640 sequence16, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVE01000016
VERSION     NZ_BMVE01000016.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces goshikiensis
  ORGANISM  Streptomyces goshikiensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 159011)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650555.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:30:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,491
            CDSs (total)                      :: 7,410
            Genes (coding)                    :: 7,286
            CDSs (with protein)               :: 7,286
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 44 of 125
            Pseudo Genes (incomplete)         :: 101 of 125
            Pseudo Genes (internal stop)      :: 9 of 125
            Pseudo Genes (multiple problems)  :: 27 of 125
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:38:35
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2917
            Orig. end                         :: 13889
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVE01000016.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10972
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10972
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10972
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5972](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5972)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    210..224
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.24"
                     /tool="antismash"
     misc_feature    complement(1871..1886)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.5"
                     /tool="antismash"
     gene            complement(2449..3507)
                     /locus_tag="IE248_RS32055"
                     /old_locus_tag="GCM10010336_65310"
     CDS             complement(2449..3507)
                     /NRPS_PKS="Domain: PKS_KS(Iterative-KS) (109-349). E-value:
                     7.7e-20. Score: 63.1. Matches aSDomain:
                     nrpspksdomains_IE248_RS32055_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) t2clf"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1093: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018960004.1"
                     /locus_tag="IE248_RS32055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_65310"
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="WP_308438199.1"
                     /sec_met_domain="ketoacyl-synt (E-value: 6.4e-13, bitscore:
                     42.1, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="t2clf (E-value: 8.4e-112, bitscore: 367.0,
                     seeds: 26, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGLGVAAPNGLGADGFWAATRTGTNGIGRITGFDPSPYPVTLAG
                     QVPGLAAEGRPPSRVLPSRLLAQSGRMPRITLVAAGWALADAGVDPEELGALDLGGLDA
                     GGAAVCAASGLDAVAQARRRIREGAGLVVSGAVDASLSPWGWGVQLVSGRLSGSAEPTR
                     AYLPFDAEAAGHVPGEGGAILILESAAAARARGARVYGEISGYGAASDPRPGSGREPGL
                     RRAIELALADAGAVPGDVDVVFADAAAIPELDRIEAEAITAVFGPCGVAVTAPKTMTGR
                     LCSGAAPLDLAAALLAIEEGLIPPTVNVRLSDAYQLDLIGPRPRTAEVRTALVLARGHG
                     GFNSAVVVRAVD"
     aSDomain        complement(2461..3180)
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE248_RS32055_PKS_KS.1"
                     /domain_subtypes="Iterative-KS"
                     /evalue="7.70E-20"
                     /label="IE248_RS32055_PKS_KS.1"
                     /locus_tag="IE248_RS32055"
                     /protein_end="349"
                     /protein_start="109"
                     /score="63.1"
                     /tool="antismash"
                     /translation="CAASGLDAVAQARRRIREGAGLVVSGAVDASLSPWGWGVQLVSGR
                     LSGSAEPTRAYLPFDAEAAGHVPGEGGAILILESAAAARARGARVYGEISGYGAASDPR
                     PGSGREPGLRRAIELALADAGAVPGDVDVVFADAAAIPELDRIEAEAITAVFGPCGVAV
                     TAPKTMTGRLCSGAAPLDLAAALLAIEEGLIPPTVNVRLSDAYQLDLIGPRPRTAEVRT
                     ALVLARGHGGFNSAVVVR"
     CDS_motif       complement(2968..3006)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE248_RS32055_0001"
                     /evalue="9.00E-03"
                     /label="PKSI-KS_m4"
                     /locus_tag="IE248_RS32055"
                     /protein_end="180"
                     /protein_start="167"
                     /score="9.5"
                     /tool="antismash"
                     /translation="FDAEAAGHVPGEG"
     misc_feature    3700..3715
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     gene            complement(3935..4969)
                     /locus_tag="IE248_RS32060"
                     /old_locus_tag="GCM10010336_65320"
     CDS             complement(3935..4969)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (2-306). E-value: 5.5e-07. Score:
                     21.6. Matches aSDomain:
                     nrpspksdomains_IE248_RS32060_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546906.1"
                     /locus_tag="IE248_RS32060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_65320"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_229895682.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 2.5e-08, bitscore:
                     26.6, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTIMITGAAGFIGGHVAREAERRGGLSLRLMSHRRPPAGAAAAG
                     PATAGPDPATAGPGSGSGFGHGHGPGAVVPGHRTVRADLSDPGSLRGVCEGVDVLLHCA
                     SQIGGTPEANEAVNARGTAALMAEARRAGVSRVVYLSTASVYGRGAFRAARPEDLVRNP
                     GSPTSGSRAHAEDAVLAAGGVVLRPHLVLGDGDRWVVPGLARILRALPGTVEGWAARTS
                     VITAQDLARLLLGAGLAPAGDLTASVYHAAHPVPVTVRTLLGAVAAVAGPAADPAAEDL
                     TAVRARELLAARGIAAQGLDMLTSDHWFDSDPVWRDLRCTPGPDFEAAFKELAEGYRRA
                     LLDI"
     aSDomain        complement(4052..4963)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE248_RS32060_PKS_KR.1"
                     /evalue="5.50E-07"
                     /label="IE248_RS32060_PKS_KR.1"
                     /locus_tag="IE248_RS32060"
                     /protein_end="306"
                     /protein_start="2"
                     /score="21.6"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="TIMITGAAGFIGGHVAREAERRGGLSLRLMSHRRPPAGAAAAGPA
                     TAGPDPATAGPGSGSGFGHGHGPGAVVPGHRTVRADLSDPGSLRGVCEGVDVLLHCASQ
                     IGGTPEANEAVNARGTAALMAEARRAGVSRVVYLSTASVYGRGAFRAARPEDLVRNPGS
                     PTSGSRAHAEDAVLAAGGVVLRPHLVLGDGDRWVVPGLARILRALPGTVEGWAARTSVI
                     TAQDLARLLLGAGLAPAGDLTASVYHAAHPVPVTVRTLLGAVAAVAGPAADPAAEDLTA
                     VRARELLAARGIAAQGLDMLTSD"
     gene            complement(5001..5972)
                     /locus_tag="IE248_RS32065"
                     /old_locus_tag="GCM10010336_65330"
     CDS             complement(5001..5972)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740559.1"
                     /locus_tag="IE248_RS32065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_65330"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_008740559.1"
                     /sec_met_domain="AfsA (E-value: 2.4e-21, bitscore: 69.0,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSLTALRIPAHHSAPAANCLLTTTVPREYVHRAAVSEVLLTGWG
                     PDPLAGRDQSDSYVVRAQWPRGHSLFAPAGGYQDPLLLIESVRQTGALLAHSEYGVPFG
                     HQFLMWDMSFIATPEALVASPVPTEVELRTVCRDVVRRGRTIASMRYEVSVWRDGVPLA
                     TASAAFSCTSPAVHRRLRGERPTTSDRTPPPAVDPAVVGRSSAPGNVVLAPAPAGPGDR
                     WELRVDTEHPIFFDHPVDHVPGMVLLESARQAAHAVTGLPDALVLGMASTFERYAELDA
                     PCWIEARAERPDAAGDIAVHVVGTQQDRPVFTADLTLRPSAG"
     misc_feature    complement(5694..5696)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    6079..6092
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.04"
                     /tool="antismash"
     misc_feature    complement(6080..6093)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.87"
                     /tool="antismash"
     misc_feature    6080..6094
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.21"
                     /tool="antismash"
     gene            6468..7259
                     /locus_tag="IE248_RS32070"
                     /old_locus_tag="GCM10010336_65340"
     CDS             6468..7259
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740558.1"
                     /locus_tag="IE248_RS32070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_65340"
                     /product="response regulator transcription factor"
                     /protein_id="WP_008740558.1"
                     /transl_table=11
                     /translation="MNRQAADVLEPRSEQIRPQRTLLSQPGHFPPPRVERPAAAAEPAL
                     RVLVVENEARAADTLVVGLRRHGYLAESVATGALALQAHGDADLILLDLDLPDLDGLEV
                     CRAIRAVGDTPLIAVTARGSELDRVLGLQAGADDYLVKPYGFRELLARMEAVMRRARPR
                     PQASRVITHGPLRIDAGTREVLLAGRPVELTRKEFDLLHLLASQPEAVISRRQLMTQVW
                     DDTWSHRGRTIDTHVSSLRGKLGASGWIVTVRGVGFRLGRP"
     gene            7365..8006
                     /locus_tag="IE248_RS32075"
                     /old_locus_tag="GCM10010336_65350"
     CDS             7365..8006
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140398.1"
                     /locus_tag="IE248_RS32075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_65350"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_047961251.1"
                     /transl_table=11
                     /translation="MAQQERGNRSRRSILEAAARVFDERGYDAASTNEILASTGLTRGA
                     LYHHFPSKEAIASALVAAQSEALVVPDQPVKLQAVIDLTLEFARRLQHDPVLRASVRLA
                     VEQTSFSRPAQTPYEQSNATILDLLRQAREQGEVLPGVDLQEATSTIVGAFTGLQVMSQ
                     VYSNRQDLPDRVCALWRFLLPGLATPGMLARLRTAPLPQTAPQAAPQTAP"
     gene            complement(8022..8609)
                     /locus_tag="IE248_RS32080"
                     /old_locus_tag="GCM10010336_65360"
     CDS             complement(8022..8609)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740556.1"
                     /locus_tag="IE248_RS32080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_65360"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_008740556.1"
                     /transl_table=11
                     /translation="MQKRSEQTRQALVSAAARIIADGRPADAGLVNICRTAGVSRGALY
                     HHFSSTTELTAAVYSQAFERATTLAEEAFEGPAESAAARFCSSLCTALRDEELVRAGTQ
                     LAADGSAGPPRLRDDALDLVRRHLVSAGGELAELADLAVVVTAGLESLGRTDGTWWEPR
                     TSERIWDLLRPLFGPRGPARGGSPLGAAGRQD"
     misc_feature    8701..8710
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: strong, score: 18.57"
                     /tool="antismash"
     misc_feature    complement(8701..8714)
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.59"
                     /tool="antismash"
     gene            complement(8886..10547)
                     /locus_tag="IE248_RS32085"
                     /old_locus_tag="GCM10010336_65370"
     CDS             complement(8886..10547)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1138: FAD linked oxidase domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740555.1"
                     /locus_tag="IE248_RS32085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_65370"
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="WP_073776718.1"
                     /transl_table=11
                     /translation="MANIARRALFRGAAATVSAVAGSAALGAVTAAPAVADEVVGEGQD
                     AMGARTTAPTGATITPNDPRYAQLTTGNNQRYVAAPSYVKMIKSAPDAAAAITAAYRAG
                     KRVSVRSGGHCFSNFVCNPDVQVILDCSEMTYVGYDPAMRAFAVEPGARLLNVYEALYK
                     GWGVTLPGGVCYGVGAGGHIAGGGYGLLSRRNGLVVDHLYAVEVVTVDASGTARIVRAT
                     REPNDPNRDLWWAHTGGGGGNFGVVTRYWLRSPNAPGGSAATPENQLVSPPSHVLMHIQ
                     GIPWSQLDEAKFSRLVKNFGAWHEANSAPNSPLRNLSSILNVYSKASGMLNLVTQVDAG
                     VPDAAAMLDTYLNQILDGVTTARAASRKLPWLQAASLIGTINTTLNDPTLRGAHKSAYM
                     RTNFTDAQLASLYASMTAADYTNPRAMLILFSFGGQVNAVAPDATAIPQRSSAFKMLYQ
                     TFWNSAAEDSTHVAWLRTLYANMHAATGGVPGLDGQTDGCYINYPDTDMADPAQNTSGV
                     PWQRLYYKGNYARLQQVKARWDPSDYFRHSMSVKQV"
ORIGIN
        1 acttcacgag cagccccgac atgccgatga cgtcggcctt gtgctgttcg gcggcttcga
       61 ggatcgcgga gacgggctgt ttgatgccga tgttggcgac gttgtagccg ttgttggaca
      121 ggatgatgtc gacgaggttc ttgccgatgt cgtggacgtc gccgcggacg gtggcgagca
      181 cgatcgtgcc cttgccgtcc gcgtcggtct tctccatgtg cggttcgagg tgggcgacgg
      241 ccgtcttcat gacctccgcc gactggagga cgaaggggag ctgcatctgg ccggagccga
      301 agagctcgcc gacgaccttc atgccctcca ggagggtgtc gttgacgatg tcgagggccg
      361 ggcgggtctg gagcgcctcg tcgaggtcgg cctccaggcc gttcttctcc ccgtcgatga
      421 tccggcggcg caggcgttcc tccaggggca gcgccatcag ttcctcggcg cggccggcct
      481 tcagtgactt ggtgttgaca ccctcgaaga gggccataag cttctggagg gggtcgtagc
      541 cctccgcccg ccggtcgtag atcaggtcga gggcggtgcc cacctgctcc tcgtcgaagc
      601 gggcgatggg gaggatcttg ctggcgtgga cgatcgcgga gtccaggccg gccttgacgc
      661 actcgtcgag gaagacggag ttgaggagga tacgggcggc cgggttgagg ccgaaggaga
      721 tgttcgagag gccgagggtg gtctggacgt ccgggtggcg gcgcttgagc tcgcggatcg
      781 ccccgatcgt ggcgatgccg tcgccccgcg actcctcctg gccggtgcag atggtgaagg
      841 tgagcgtgtc gatgaggatg tcggattcga ggatgcccca gttctcggtg aggtcctcga
      901 tcagccgctc cgcgatctcc accttgtgct cgacggtacg ggcctgtccc tcctcgtcga
      961 tggtcagcgc gatcagcgcg gcgccgtgct cccgcgccag ccgggtgacc ttggcgaagc
     1021 gcgagtcagg gccgtcgccg tcctcgtagt tgacggagtt gatgacggcg cggccgccga
     1081 gcttctccag gcccgcctgg atgacgggaa cctcggtgga gtccaggacg atcggcagcg
     1141 tggaggcggt ggcgaaccgg ccggcgagtt ccgccatgtc ggcgacgccg tcccgcccga
     1201 cgtagtccac gcacaggtcc agcatgtgcg cgccctcgct gatctggtcc cggaccatct
     1261 ccacgcagtc gtcccagcgg gcctccagca tggcctcgcg gaactgcttc gacccgttgg
     1321 cgttcgtccg ctcgccgatc gccatgtacg aggtgtcctg ccggaacggg accgtctggt
     1381 acagcgaggc cgcaccgggc tccgggctcg gttcgcgcac cgccggggcc agcccgcgga
     1441 cccgctcgac gacctggcgc aggtgctccg gggtggtgcc gcagcagccg ccgaccaggg
     1501 agagaccgta ctcgcggacg aaggactcct gggcgtcggc gagctcgctc gccgacagcg
     1561 ggtagtgcgc gccgtccttc gtcagcaccg gcaggccggc gttcggcatg caggacagcg
     1621 ggatccggga gttgcgggcg aggtagcgca ggtgctcgct catctccgcg gggccggtgg
     1681 cgcagttcag cccgatcatg tcgatgccga ggggttcgag ggcggtgagg gccgcgccga
     1741 tctcggagcc cagcagcatg gtgccggtgg tctcgacggt gaccgagcag atcagcggga
     1801 ccgagaggcc gagggcctcc atcgcccgcc gggcgccgat gacggaggac ttggtctgga
     1861 ggaggtcctg ggtggtctcc accagcagcg cgtccgcgcc gcccgtcagg agcccctccg
     1921 cgttcgcctg gtaggcgtcg cggatcgtcg cgtagtcgat gtggccgagg gtggggagct
     1981 tggtgccggg gcccatcgag ccgaggaccc agcgctgctg cccggtggcg gcggtgaact
     2041 cgtccgcggc ctcgcgggca atccgcgcgc ccgcctccga cagctcgaag ttgcgctccg
     2101 cgatgtcgta ctcggcgagg gcggcgaagt tcgcgccgaa ggtattggtc tcgacgcagt
     2161 ccacgccgac ggagaaatac gcctcgtgga ccgtccggac gatatcgggg cgggtgacgt
     2221 tcagaatctc gttgcagcct tcgaggttct ggaaatcatc gagggtgggg tcctgcgcct
     2281 ggagcatggt gcccatggcg ccgtcggcga ccaccacacg ggtcgcgagc gcctcacgga
     2341 gggcattgga gcggttccgg ctgtcaccgg aaagggttgg cagcgaggcc atggaaaagc
     2401 tcctgttcaa cgagagggcc gctcaatgtg aaagaacgtc gaataccgtc agtccacggc
     2461 gcggaccacc acggccgagt tgaagccgcc gtgaccgcgc gccaggacca gcgcggtgcg
     2521 cacctcggcg gtgcgcggcc ggggtccgat caggtccagc tggtacgcgt cggacagcct
     2581 cacgttgacc gtgggcggga tcaggccctc ctcgatggcc agcagcgcgg ccgccaggtc
     2641 gagcggggcg gcgcccgaac acagccggcc cgtcatcgtc ttgggcgcgg tgaccgcgac
     2701 gccgcagggg ccgaacaccg cggtgatggc ctcggcctcg atccggtcca gctcggggat
     2761 ggcggccgcg tcggcgaaga ccacgtccac gtcgccgggt acggctcccg cgtcggccag
     2821 ggccagctcg atcgcccggc gcaggcccgg ctcgcgcccg ctgccgggcc gggggtcgga
     2881 cgccgctccg tagccggaga tctccccgta gacgcgggcg ccgcgcgccc gggccgccgc
     2941 ggcggactcc aggatcaaga tcgcgccgcc ctcaccgggg acgtgtccgg ctgcctcggc
     3001 gtcgaagggg aggtaggcgc gggtgggctc cgcgctgccg ctcagccggc cgctgacgag
     3061 ctggacgccc cagccccagg ggctgagcga ggcgtccacg gcgccggaga cgaccaggcc
     3121 ggcgccctca cggatgcggc ggcgggcctg cgcgacggcg tcgagcccgc tcgccgcgca
     3181 gaccgcggcg ccgccggcgt ccagcccgcc gaggtcgagc gcgccgagct cctcggggtc
     3241 cacgcccgcg tcggcgagcg cccagcctgc ggcgacgagc gtgatccggg gcatccggcc
     3301 gctctgcgcc agcagccggc tcggcagtac ccggctcggc ggccgcccct cggcggcgag
     3361 gccggggacc tggccggcga gggtcaccgg gtacggggag gggtcgaagc cggtgatccg
     3421 gccgatgccg ttcgtaccgg tgcgggtcgc ggcccagaag ccgtccgcgc cgagtccgtt
     3481 gggggcggcg acgcccaggc cggtcacgac caccgaggtg gccgtgccgc gttccgcccg
     3541 ggacgcggcg gccgggtggc acagggggat ccggcccagc cgtcgccggc agccggggcc
     3601 gtggtggaag gccgggcgcg aacgtccggc gggccgtaag cgggatcgcg ttccgggccg
     3661 ggagtgggcg gattcgcggt cggcgggacg ccgcgatccg gtgaagggtt tcacggcacc
     3721 gattgccggt ggggggtccg tccggaccac tgtcagcgtg ccgtgcatac cttccctctc
     3781 ctttgaccca ccgcgaaaag aatgccggag cacgatgtac agcgtgttcc gtcacgggcc
     3841 gcggttcaat acggggccgt gcagaactcg gtcaggtttc cgtaaggcca ttgcatacgt
     3901 gtcgggttca gttcaggaga agtccagggg aatgtcagat gtcgagcagg gcgcggcggt
     3961 agccctcggc gagttccttg aacgcggcct cgaaatccgg gccgggcgta caccgcaggt
     4021 cccgccagac cgggtcgctg tcgaaccagt gatcgctcgt cagcatgtcg agcccctggg
     4081 ccgcgatgcc ccgggcggcg agcagctcgc gggcgcgcac cgccgtgagg tcctcggcgg
     4141 ccgggtccgc cgccgggccc gcgacggcgg cgaccgcgcc gagcagggtg cgtacggtga
     4201 cgggcacggg gtgggcggcg tggtagacgg acgccgtcag gtccccggcc ggggcgaggc
     4261 cggcgccgag caggagccgg gccaggtcct gcgcggtgat gacggacgta cgggcggccc
     4321 agccctcgac ggtgcccggc agggcccgca ggatccgggc caggcccggg accacccagc
     4381 ggtcgccgtc gccgaggacg aggtgcgggc gcaggacgac cccgcccgcc gcgaggacgg
     4441 cgtcctcggc gtgggcgcgc gagcccgagg tgggcgagcc cgggttgcgg acgaggtcct
     4501 cgggccgggc ggcgcggaag gcgccgcggc cgtacacgga ggcggtgctg aggtagacga
     4561 cgcgggagac cccggcgcgc cgggcctcgg ccatcagggc ggccgtaccg cgggcgttga
     4621 cggcctcgtt ggcctcgggg gtgccgccga tctgcgaggc gcagtgcagc agtacgtcga
     4681 ccccctcgca gacgccccgc agcgagccgg ggtccgagag gtcggcgcgc acggtccggt
     4741 ggccggggac cacggcgccg gggccgtggc cgtggccaaa gccggagcca gagccggggc
     4801 cggccgtggc ggggtcaggg ccggccgtgg cggggccggc cgcggccgcg ccggccggcg
     4861 gtcttcggtg ggacatcagc cgcagggaca ggccgccccg gcgctcggcc tcgcgggcga
     4921 cgtgtccgcc gatgaatccg gccgctcccg tgatcatgat ggtgggcacg cgcgctctcc
     4981 cggtggtggt gatggcgggc tcagcccgcg ctggggcgca gggtgaggtc ggcggtgaag
     5041 accggccggt cctgctgggt gccgacgacg tggacggcga tgtcccccgc cgcgtcgggg
     5101 cgttcggcgc gggcctcgat ccagcagggg gcgtcgagtt cggcgtaccg ctcgaaggtg
     5161 ctcgccatgc cgaggacgag ggcgtccggc agtccggtga cggcgtgggc ggcctggcgg
     5221 gccgactcca gcagcaccat gccggggacg tggtcgacgg ggtggtcgaa gaagatcggg
     5281 tgctcggtgt cgacccggag ctcccaccgg tcgcccggac ccgccggggc gggggcgagg
     5341 acgacgttgc cgggggcgga ggacctgccg accacggccg ggtcgacggc cggcggcggg
     5401 gtgcggtccg aggtggtggg gcgctcgccg cgcagccgcc ggtgcacggc cgggctggtg
     5461 cagctgaagg cggcggaggc ggtggcgagc ggcactccgt cgcgccacac cgagacctcg
     5521 tagcgcatgc tcgcgatggt gcggccgcgg cggacgacgt cgcggcacac ggtgcgcagt
     5581 tcgacctcgg tgggcacggg gctcgccacg agcgcctcgg gggtggctat gaaggacatg
     5641 tcccacatca ggaactggtg cccgaagggg accccgtact cggagtgcgc gagtaaggcc
     5701 ccggtctggc ggaccgactc gatcagcagc agcgggtcct ggtagccgcc ggccggggcg
     5761 aagagggagt ggccgcgcgg ccactgcgcc cgcacgacgt aggagtcgct ctggtcgcgg
     5821 ccggcgagcg ggtcgggtcc ccagccggtc agcagtactt cgctgacggc cgcgcggtgc
     5881 acgtactccc ggggcaccgt ggtggtgagc aggcagttcg ccgccggagc gctgtggtgg
     5941 gcggggatac ggagagcggt gagggacgac atgcgctgta ctcctgttct gcttgaagag
     6001 caggggtgag ccacgcacac gtcggcagcc ccccgctgtg atcgcttgca gacctctaag
     6061 atacagacag gtcggttctt ttcttaagga aatcttcgag ttttccgtaa caaccgctga
     6121 cccggaccga cggaatcccg ccaagaggtc ggttcctccc gcgcgagcgc cgccggaacc
     6181 cttgactccg gccccaactc cactggaaca gacagcactg ccgggtttac gcgcccgcaa
     6241 cgcgggtcgc cgcacctcat ctccgatcac ggaaaacgac caaaccgatg acccaaaaca
     6301 ccctattttc acgatacttg ctgttcagag aggcctcccg gcgacccgac caacaccaac
     6361 gcgcggagcc cgcgctcccg ttgacaatca gacaagtctg tttggtatct tcattcagca
     6421 cgagcgttca gcagtccgat ggcgcgactg cgcctcagga ggagcagatg aatcggcagg
     6481 ccgcggatgt cctcgaaccc cgctcggagc agatccgccc ccagcgcacc ctgctttccc
     6541 agccgggcca cttcccgccg ccgcgggtgg agcggcccgc cgccgcggcc gaaccggccc
     6601 tgcgggtcct cgtcgtcgag aacgaggcgc gcgcggccga caccctggtg gtggggctgc
     6661 gccggcacgg ctacctggcc gagagcgtgg ccaccggcgc cctcgccctc caggcgcacg
     6721 gcgacgccga cctgatcctc ctcgacctcg acctgcccga cctcgacggc ctggaggtct
     6781 gccgcgccat ccgcgccgtc ggcgacaccc cgctgatcgc ggtcaccgcg cgcggcagcg
     6841 agctcgaccg ggtgctcggg ctccaggccg gggcggacga ctacctcgtc aagccgtacg
     6901 gtttccgcga actgctcgcg cggatggagg cggtgatgcg ccgggcccgc ccccggccgc
     6961 aggccagccg ggtcatcacg cacgggccgc tgcgcatcga cgccggcacc cgcgaggtcc
     7021 tgctggccgg ccggccggtg gagctgacgc gcaaggagtt cgacctgctc catctgctcg
     7081 cctcgcagcc ggaggcggtc atctcccgcc gccagctgat gacgcaggtg tgggacgaca
     7141 cctggtcgca ccgcggccgc accatcgaca cccacgtcag cagcctgcgc ggcaagctcg
     7201 gggcgagcgg ctggatcgtc acggtgcgcg gcgtcgggtt ccgcctcggc cggccgtagc
     7261 ccctcccggg cgcccgggag gggccgcccg gggcccctcg ttccgcagac cgatacgatt
     7321 ccgacaggtc ggtttggttc cagtcagtaa cgcaaggacg tagcatggca cagcaagaac
     7381 gcggcaaccg gtcgaggcgg tcgatcctgg aggccgccgc gcgcgtcttc gacgagcgcg
     7441 ggtacgacgc cgcgagcacg aacgagatcc tcgcgagcac cggtctgacc aggggggcgc
     7501 tctaccacca cttcccctcg aaggaggcga tcgcctccgc gctggtggcc gcgcagagcg
     7561 aggcgctggt ggtgcccgac cagcccgtca agctccaggc cgtcatcgac ctcacgctgg
     7621 agttcgcgcg ccggctccag cacgacccgg tgctgcgggc gagcgtacgg ctggcggtgg
     7681 agcagacctc cttctcgcgc ccggcgcaga ccccgtacga gcagtcgaac gcgaccatcc
     7741 tcgacctgct gcgccaggcg cgggaacagg gtgaggtgct gcccggcgtg gacctccagg
     7801 aggccacgtc cacgatcgtg ggggccttca ccggcctcca ggtgatgtcg caggtgtaca
     7861 gcaaccggca ggacctgccg gaccgggtct gcgcgctgtg gcggttcctg ctgcccggac
     7921 tggccacgcc cggcatgctc gcccggctgc gcacggcgcc gctgccgcag accgcaccgc
     7981 aagccgcgcc gcagaccgcg ccgtagcccc cgcgcgcccc gtcagtcctg acggcccgcc
     8041 gcccccaggg ggcttcctcc ccgcgccggc ccccgggggc cgaacagggg gcgcagcagg
     8101 tcccagatcc gctccgaggt gcgcggttcc caccacgtgc cgtcggtgcg ccccaacgac
     8161 tccagcccgg cggtgaccac cacggcgagg tcggccagtt cggccagttc cccgccggcc
     8221 gagaccaggt gacggcggac caggtccagg gcgtcgtccc gcagccgcgg cggcccggcg
     8281 ctgccgtccg ccgccagctg cgtgcccgcc cgtacgagtt cctcgtcgcg cagcgcggtg
     8341 cacagcgagg aacagaaccg ggcggcggcc gactcggcgg ggccttcgaa ggcctcctcc
     8401 gccagggtcg tggcccgctc gaaggcctgg ctgtacacgg cggcggtcag ctccgtggtg
     8461 gaggagaagt ggtggtagag cgctccgcgg ctcactccgg cggtgcggca gatgttcacc
     8521 agcccggcgt cggcgggccg gccgtccgcg atgatcctcg ccgcggcgga gaccagggcc
     8581 tggcgggtct gttcggaacg cttctgcatg gcgcggcacc tgcatctctc tcttcacggc
     8641 acacagaagc gcgggggacc gccgggccgt cggcactaca ccgaatcata caaccctgcc
     8701 tgttttttga aacccctcgc ccaacgagag cccctttaca gcccctttga cctccccgga
     8761 tgcttggtat tcgctgattt cgcgcagact gattcagtaa caaccagaca agtctgttcg
     8821 ttatgacggc gtcggccggc gccgtgacct tccctcaccg gccgacgcgg gagcactccg
     8881 gccggtcaga cctgcttcac ggacatcgag tgccggaagt agtcggaggg gtcccacctg
     8941 gccttgacct gctgcagccg cgcgtagttg cccttgtagt acagccgctg ccacggcacg
     9001 ccggaggtgt tctgcgccgg atcggccatg tcggtgtccg ggtagttgat gtagcagccg
     9061 tcggtctggc cgtcgaggcc cgggaccccg cccgtcgccg cgtgcatgtt cgcgtacagg
     9121 gtgcgcagcc aggcgacgtg cgtgctgtcc tcggccgcgc tgttccagaa cgtctggtag
     9181 agcatcttga aggccgagct gcgctgcggg atcgccgtgg cgtcgggcgc gaccgcgttc
     9241 acctggccgc cgaaggagaa caggatcagc atggcgcgcg ggttcgtgta gtccgccgcc
     9301 gtcatcgacg cgtacagcga ggcgagctgc gcgtccgtga agttggtgcg catgtacgcc
     9361 gacttgtggg ccccgcgcag cgtggggtcg ttgagcgtgg tgttgatggt gccgatgagg
     9421 ctggcggcct ggagccacgg cagtttgcgg ctcgcggcgc gcgcggtggt gaccccgtcg
     9481 aggatctggt tgaggtaggt gtcgagcatc gcggccgcgt cggggacgcc ggcgtccacc
     9541 tgggtgacca ggttcagcat cccgctggcc ttggagtaga cgttcaggat gctgctcagg
     9601 ttgcgcagcg gcgagttggg ggcgctgttc gcctcgtgcc aggcgccgaa gttcttcacg
     9661 agccggctga acttggcctc gtccagctgg ctccagggaa tgccctggat gtgcatcagg
     9721 acgtgggagg gcggggagac gagctggttc tccggggtgg ccgcggagcc gccgggggcg
     9781 ttcggggagc gcagccagta gcgcgtgacc acgccgaagt tgccgccgcc gccgcccgtg
     9841 tgcgcccacc acaggtcccg gttggggtcg ttcggctcgc gggtggcccg cacgatgcgg
     9901 gcggtgccgg aggcgtcgac ggtgacgacc tcgacggcgt agaggtggtc gacgaccagg
     9961 ccgttgcggc gggagaggag accgtagccg ccgccggcga tgtggccgcc cgcgccgacg
    10021 ccgtagcaaa cgccgccggg gagggtgacg ccccagccct tgtacagggc ctcgtagacg
    10081 ttgaggagac gggcgccggg ctcgacggcg aaggcccgca tggccgggtc gtagcccacg
    10141 tacgtcatct cggagcagtc gaggatgacc tggacgtccg ggttgcagac gaagttgctg
    10201 aagcagtggc cgccgctgcg caccgagacc cgcttgccgg cccggtacgc ggcggtgatg
    10261 gcggcggccg cgtcgggcgc cgacttgatc atcttcacgt acgagggcgc ggcgacgtac
    10321 cgctggttgt tgccggtggt gagctgcgcg taccgcgggt cgttcggggt gatcgtggcc
    10381 ccggtcggcg ccgtcgtacg cgcccccatg gcgtcctggc cctcgcccac gacctcgtcg
    10441 gcgacggccg gggcggcggt gacggcgccg agcgccgcgc tgccggcgac ggcggagacg
    10501 gtggcggcgg cgcctctgaa gagcgcccgt ctggcgatgt tggccatgct ggtttccccc
    10561 ttggatgtga cggtgtgtgg gaggggcccc cggctgctgc cgggggcccg tccggggtag
    10621 gagtcggatg cgggacgtcg ggaacgggaa aggaccgccg gggcccccgg tggccggggg
    10681 ccggggcggc tagcccttgg gggccgggcc gttgcccagc gacgaggcgc cgctggcgtc
    10741 gatgacctgg ccggagaccc agccggcctc gccggacgcg aggaacgcgg cgatgcccgc
    10801 cacgtccgcc ggggtgccgg tgcggccgaa cgccgaccag ctctcggccg cggcccgcat
    10861 ctgcgggtac gccagccagg ggttgaagtc ggtgtcgatg atgccggggg cgatcgcgtt
    10921 gacggtgatc ccccggggcg cgagcgcctc ggcgagggtc agggtcaggg tg
//
