LOCUS       NZ_BMVE01000002        11344 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces goshikiensis strain JCM 4640 sequence02, whole genome
            shotgun sequence.
ACCESSION   NZ_BMVE01000002
VERSION     NZ_BMVE01000002.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces goshikiensis
  ORGANISM  Streptomyces goshikiensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 1126124)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650555.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:30:19
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,491
            CDSs (total)                      :: 7,410
            Genes (coding)                    :: 7,286
            CDSs (with protein)               :: 7,286
            Genes (RNA)                       :: 81
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 124
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 44 of 125
            Pseudo Genes (incomplete)         :: 101 of 125
            Pseudo Genes (internal stop)      :: 9 of 125
            Pseudo Genes (multiple problems)  :: 27 of 125
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 18:38:35
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 307270
            Orig. end                         :: 318614
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMVE01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11344
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11344
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11344
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6344](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6344)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            1331..2044
                     /gene="hemQ"
                     /locus_tag="IE248_RS06695"
                     /old_locus_tag="GCM10010336_13630"
     CDS             1331..2044
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888541.1"
                     /locus_tag="IE248_RS06695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_13630"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_037798306.1"
                     /transl_table=11
                     /translation="MTAPEKIPNAGKKAKDLNEVIRYTLWSVFKLKDVLPQDRAGFADE
                     VQELFDQLAAKDITVRGTYDVSGLRADADIMIWWHAETSDELQTAYNLFRRTRLGRALE
                     PVWSNMALHRPAEFNKSHIPAFLADEVERDYVSVYPFVRSYDWYLLPDEDRRRMLADHG
                     KMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARMHVREEVPFY
                     TGRRKSVADLVDGLA"
     gene            complement(2159..3835)
                     /locus_tag="IE248_RS06700"
                     /old_locus_tag="GCM10010336_13640"
     CDS             complement(2159..3835)
                     /NRPS_PKS="Domain: Abhydrolase_1 (116-485). E-value:
                     1.2e-23. Score: 75.9. Matches aSDomain:
                     nrpspksdomains_IE248_RS06700_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643866.1"
                     /locus_tag="IE248_RS06700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_13640"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_229895158.1"
                     /sec_met_domain="PF00561 (E-value: 3.2e-30, bitscore:
                     101.4, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRMPLVRPAVAATALAFALTLTLAPAAPAAPAPAPAPATAVAAEN
                     AGAERVARRVAAGHRALDFRSCPAAEGLAAPVRCAALRVPLDYARPDGPQISLTVSRVG
                     ATREGRAARQGSLVFNPGGPGASGMYFPLLADRPAWRRVAAAYDLVGYAPRGVGRSAPL
                     SCQDPAGRPKAPTQVPADPSAAYKRERVAAARAYARGCARRGGAALPYYTTLNNARDLH
                     VLRAALGERELTFVGASYGTYLGAVYATLYPGHVRRMVFDSAVDPDPRHVWYRDNLAQA
                     AGFERRWYDFRAWAARHHATYRLGATPAAVRASHERVRAAVARTPAGGVAGTGELRAAA
                     LRAAYSDEVWPELAAALSAFLDGDPAPLAALAGPDPDPVVAARAAAEAENATALYTAVL
                     CNDAPWPADWETWDRDHTELARRAPLETWANAFLNLPCAYWPVAQRLRQRPVDVGSRAT
                     PLPPTLIVAAERDGATPYPGALELRRRLGGAAALVTEAGAGAHGVAGGRNDCVDRHVER
                     YILTGDTPKGSVTCAPHPEPAPVSLDDRAAGTRRVPLPPVV"
     aSDomain        complement(2381..3487)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE248_RS06700_Abhydrolase_1.1"
                     /evalue="1.20E-23"
                     /label="IE248_RS06700_Abhydrolase_1.1"
                     /locus_tag="IE248_RS06700"
                     /protein_end="485"
                     /protein_start="116"
                     /score="75.9"
                     /tool="antismash"
                     /translation="LVFNPGGPGASGMYFPLLADRPAWRRVAAAYDLVGYAPRGVGRSA
                     PLSCQDPAGRPKAPTQVPADPSAAYKRERVAAARAYARGCARRGGAALPYYTTLNNARD
                     LHVLRAALGERELTFVGASYGTYLGAVYATLYPGHVRRMVFDSAVDPDPRHVWYRDNLA
                     QAAGFERRWYDFRAWAARHHATYRLGATPAAVRASHERVRAAVARTPAGGVAGTGELRA
                     AALRAAYSDEVWPELAAALSAFLDGDPAPLAALAGPDPDPVVAARAAAEAENATALYTA
                     VLCNDAPWPADWETWDRDHTELARRAPLETWANAFLNLPCAYWPVAQRLRQRPVDVGSR
                     ATPLPPTLIVAAERDGATPYPGALELRRR"
     gene            complement(3949..5004)
                     /locus_tag="IE248_RS06705"
                     /old_locus_tag="GCM10010336_13650"
     CDS             complement(3949..5004)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956906.1"
                     /locus_tag="IE248_RS06705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_13650"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_190030165.1"
                     /transl_table=11
                     /translation="MNLLAWAVWIAVITSTVLLLRGLRRSRPAPGGPAHPPLDLSEAAF
                     MAGGPGAVVDAALVSLLGDQRMVAGGPGIVQVRPGARAVDVAERAVLQAYANAPSGWLY
                     QVRYAAMTDPAIQEIGDALAERGLISPPGAARRWRRWGLAQAVLCGALLPVSLVLTFVG
                     FALEPGFQLPFLVKVFPVLIGGLVVGVAAAARAKRRITPAGRTALRAMRATHLTDQTPH
                     VQTALFGLRGLRDPYLREQLVPAARSTRLAAAQAGQRRSAGSPGSRSSWGSSSADALPV
                     VWCASVDGGSGGGSSGCGGSSGCGGSSGSGCGSSGSGCGSSGSSCGSSSSSCSSGGSSC
                     GSSSSSCSSSS"
     gene            complement(5001..6344)
                     /locus_tag="IE248_RS06710"
                     /old_locus_tag="GCM10010336_13660"
     CDS             complement(5001..6344)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037016.1"
                     /locus_tag="IE248_RS06710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_13660"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_190030166.1"
                     /sec_met_domain="DUF692 (E-value: 8.2e-91, bitscore: 299.7,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKPMAHLGVGIGWRPEIADAVERLPGLDWVEVVAENICPGHLPAS
                     LLRLRERGVRVVPHGVSLGLGGAHRPDPEKLAALGERAVALGAPLVTEHIAFVRTTTPA
                     PALEAGHLLPVPRTRDALDVLCENVRIAQDALPVPLALENIAALISWPGEEMTEGRFLS
                     ELVERTGVRLLIDVANLHTNRVNRGEDPAAVLDAIPLEALAYVHVAGGFERDGVWHDTH
                     AHPVPGVVLDVLAQLRRRVDPPGVLLERDDDFPPEGELAGELEAIRGVLDPAGSRPSAS
                     LRTPSRHSAPEPAPDPIPDAAPVPAADPVPDAARTRVGLAQAALLSALVAGTPVPEGFD
                     RQRIRVQSRSLAAKRAGVVAKVAPELPGILGGEKAYREAFLAYARNRPMTGGYRRDALD
                     FAEHLLIRELPADPSDRRRLTAWWQERAGTRPARRIVRWARALVGRAA"
     gene            complement(6459..7226)
                     /locus_tag="IE248_RS06715"
                     /old_locus_tag="GCM10010336_13670"
     CDS             complement(6459..7226)
                     /EC_number="3.1.1.29"
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130509.1"
                     /locus_tag="IE248_RS06715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_13670"
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="WP_229895159.1"
                     /transl_table=11
                     /translation="MNSAMSSPDTHHDDSTPVPAAGAEHPPRPQRSARDEAPQFVLPLV
                     VRIEKAEPPARTDALETAARAVLVLLSDERALGEGEWAGAVRDWQDARIRKVVRRARGA
                     EWRKAETLPGVTVHGDAAEVRVFPPVPLDGWPKELAKLQVSGTDLDDPEPVAPAPDGIA
                     VLWLNPDLEMSAGKAMAQAGHAAQLAWWELTAAERTAWQDSGFRLAVRTAPRERWARLS
                     GSGLPVVRDAGFTEIAPGSATVVSDHPALRARL"
     gene            7322..7996
                     /locus_tag="IE248_RS06720"
                     /old_locus_tag="GCM10010336_13680"
     CDS             7322..7996
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267203.1"
                     /locus_tag="IE248_RS06720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_13680"
                     /product="AIM24 family protein"
                     /protein_id="WP_037798308.1"
                     /transl_table=11
                     /translation="MKSDLFASEHLAQPSAAPGMTLQNAKSVKYTVNGEMLARQGSMVA
                     FRGNLQFERKGQGIGGMLKRAVTGEGLALMSVRGQGDAWFAHQAGNCFIIDFEPGDALT
                     INGRNVLCFDPTLSYEIKMVKGAGMTGGGLFNSVFTGTGKLAVVCDGHPIIIPVTAQNP
                     VYVDTDAVVGWSAQLETGLHRSQSVGSMIRGGSGEAVQLMLRGEGFVIVRPSELTETAA
                     AH"
     gene            8038..8853
                     /locus_tag="IE248_RS06725"
                     /old_locus_tag="GCM10010336_13690"
     CDS             8038..8853
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267204.1"
                     /locus_tag="IE248_RS06725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_13690"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_073796532.1"
                     /transl_table=11
                     /translation="MRLDAVGRRYGVRGPWVLRGVSLELPPGALVRVEGRNGGGKSTLL
                     RLVAGIDTPTEGSITGRPRRTAYVPERFPAALPLTAQAYLGHLGRVHGLTRAEAARRAA
                     QWLERFGAAGHARTPLAELSKGTSQKVAVAQALLAGPGLLVLDEAWTGLDVPARAELDR
                     AVAERTAAGGTVLFVDHDPARLAGAPDRHYRVEGGALEPVRPGAGPGPRVRIEAAGPPG
                     AVPPPGLAAEPLPAGGFRLTVPAGRSDAVLRELLTAHPAWHIRNVSDLP"
     gene            8850..9488
                     /locus_tag="IE248_RS06730"
                     /old_locus_tag="GCM10010336_13700"
     CDS             8850..9488
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452202.1"
                     /locus_tag="IE248_RS06730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_13700"
                     /product="ABC transporter"
                     /protein_id="WP_190030167.1"
                     /transl_table=11
                     /translation="MTALLRYQGALLLRSQRWLAPVLLYAAFLAVGFRPGDPVPDSLGY
                     SAAALVPVTAWLVRTCLSVEPDAARDCAAAAAGPVRVHAAALLTGLGWGAGAGGLGAVA
                     VLLLGAREGPRLPTAALAGAASVLVCALTGAAVGALCARPLLRAPGYAVAAAGLGSLLA
                     LVTAGSPARVAVSGLVGGSVALPLGALAGAAALACAAGAAACLAAARRG"
     gene            9503..9694
                     /locus_tag="IE248_RS06735"
                     /old_locus_tag="GCM10010336_13710"
     CDS             9503..9694
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997022.1"
                     /locus_tag="IE248_RS06735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_13710"
                     /product="hypothetical protein"
                     /protein_id="WP_100580480.1"
                     /transl_table=11
                     /translation="MDQCEPTESYCPAPAAPARVVAGPPYADCLECGKPTEYGVRTPGV
                     VLCPVCEWQEAQRTACSG"
     gene            complement(9717..10649)
                     /locus_tag="IE248_RS06740"
                     /old_locus_tag="GCM10010336_13720"
     CDS             complement(9717..10649)
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019063345.1"
                     /locus_tag="IE248_RS06740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010336_13720"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_229895160.1"
                     /transl_table=11
                     /translation="MTSSLRKAAAAAVLSGALGAVLAGCGGGAGPSNGGAGQARLGASA
                     TPSASASASARASAPAPAAGAASALPRAGAPGKAPTLAPGPGGLTPVFERARQKTEKTV
                     ALTFDADMTSDQGPRAAGGEHFDNPQLIDTLRALKVPSTVFMTGRWAEEYPDQAKAIGT
                     DPNFEIANHSYSHHAFKSPCYGLPTLDGAAARADVDRAFDAFRKAGAVNTVPYFRFPGG
                     CYDDQALRALSTAKVTAVQWDVVSGDAFAKDPDAVAEQVLAGVKPGSLVVMHCTRSAAP
                     VTEEAIRAIVPELRKRGYRFVKVSELMGT"
     misc_feature    complement(10654..10663)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 12.62"
                     /tool="antismash"
     misc_feature    complement(10657..10671)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 14.73"
                     /tool="antismash"
ORIGIN
        1 tgcgctccgg tgaactcgcc ggcgcccccg tcgacctcgg cgccgaatcc gtgctcgccc
       61 gccgccccga agccgtggcg ctcgcccggg ccgtcggcct cggcgaggcc ctccagccgc
      121 ccgccaccgc caccgcccag ctgtggaccc ggggcgcgct gcggcccatg ccgcgcggcc
      181 atgtcatggg cgtccccggc gacttggcgc cgctggccgc ctccggggtg ctctccgccg
      241 agggcctggc ccggatcgag gccgagcgcg cgctgccgcc cgccgagatc ggcgaggaca
      301 tcgccatcgg cgcgtacgtc gccgaccggt tcggccgcga ggtcgtcgac cggctcgtcg
      361 aaccgctgct cggcggcgtc tacgcgggca acgcctaccg gatctccatg cgggccgccg
      421 tgccccagct cttcgaggcc gcccgcacgc acccgctgct cggcgacggc gtacgggaca
      481 tccagcgccg cgccgcggag cagccccggg cgggcggccc cgtgttcgcc ggcatcgacg
      541 gcggcatcgg gcgcctcccg ctcgccgtgg cgcgggcctg ccgcgaggcc ggtgcgcgga
      601 tcagcaccgg gaccccggtg cgcgagttct tccgtaccac cgcgggctgg cgggtcgtcg
      661 ccgacggcgg cgaggtcatg gacgccgacg ccgtggtcct cgccaccccg gccgggcccg
      721 ccgcccggct gctggacgcg ctcgccccgg ccgccgccgc cgagctgcgc accgtcgagt
      781 acgcctcgat ggccctggtc acgatggcct tccggcgctc cgagctgccc gccgctatcg
      841 ccgggggcgg ggccagtgga ttcctcgtac cgcccgtgga cggccgtacg atcaaggcgt
      901 ccaccttctc cagcaacaag tgggcctggg cgggcgccga ccccgatctg ttcctgctgc
      961 gcacctcggt gggccgccac ggcgacgagg ccgacctcgg ccgggacgac gccgatctgg
     1021 tggaggtctc cctgcgggac ctcggcgagg ccgtcggcct ggcggcccgg ccggtggcct
     1081 ccaccgtcac ccgctgggag ggcgggctgc cccagtaccc ggtgggccac ctggcccgcg
     1141 tggaccggat ccgggccgcc gtcgcggccc tgccgggcct cgccgtgtgc ggcgccctct
     1201 acgacggcgt gggcatcccc gcctgcgtgg cgagtgccac ccgggccgcc gacgtggtga
     1261 tggccacgtt gggcgccccg ttgggcaccc ctggcgccga ccactgatca gcacacggga
     1321 caatggacac atgactgcac cagagaagat tcccaacgcg gggaagaagg cgaaggacct
     1381 caacgaggtc atccgctaca ccctgtggtc cgtcttcaag ctgaaggacg tcctgccgca
     1441 ggaccgcgcc ggtttcgccg acgaggtcca ggagctgttc gaccagctgg ccgccaagga
     1501 catcaccgtc cgcggcacct atgacgtctc cggcctgcgc gccgacgcgg acatcatgat
     1561 ctggtggcac gccgagacct cggacgagct gcagaccgcc tacaacctgt tccgccgcac
     1621 ccggctcggc cgcgcgctgg agccggtgtg gtcgaacatg gccctgcacc gcccggccga
     1681 gttcaacaag tcgcacatcc cggccttcct ggccgacgag gtcgagcgcg actacgtcag
     1741 cgtctacccg ttcgtgcgct cgtacgactg gtacctgctg cccgacgagg accgtcgccg
     1801 gatgctcgcc gaccacggca agatggcccg cggctacccg gacgtgcgcg ccaacaccgt
     1861 cgcgtccttc tcgctgggcg actacgagtg gatcctggcc ttcgaggccg acgagctgta
     1921 ccgcatcgtc gacctgatgc gtcacctgcg cgcctccgag gcccgtatgc acgtccgcga
     1981 agaggtgccc ttctacaccg ggcgccgcaa gtccgtcgcc gatctggtgg acgggctcgc
     2041 ctgagacctc ccctgggggg aaggaccgga agaccgctct cgtgactgag gaggggcttc
     2101 cggtggatcg ggaggccttg cccccgaaac gacgcagccg gagatcccgc ccggaagttc
     2161 agacgactgg cggcagcggc accctgcggg tgcccgctgc ccggtcgtcc agcgacaccg
     2221 gtgcgggctc cggatgcggc gcacacgtga cactcccctt gggggtgtca ccggtcagga
     2281 tgtaccgctc cacgtgccgg tccacgcagt cgttgcgtcc gccggccact ccgtgggcgc
     2341 cggcaccggc ctccgtgacc agcgcggccg ctccgcccag ccgccgcctc agctccagcg
     2401 cacccgggta cggggtcgcg ccgtcccgct ccgccgcgac gatcagcgtc ggtggcaggg
     2461 gagtcgcccg cgagcccacg tcgaccgggc gctgccgcag ccgctgggcc accggccagt
     2521 acgcgcacgg caggttcagg aaggcgttcg cccaggtctc caggggcgcc cggcgggcca
     2581 gttcggtgtg gtcgcggtcc caggtctccc agtcggccgg ccagggggcg tcgttgcaca
     2641 gcaccgccgt gtagagcgcc gtggcgttct cggcctcggc cgccgcgcgc gccgccacca
     2701 ccgggtccgg gtcgggcccg gccagggcgg ccagcggggc cggatcgccg tccaggaagg
     2761 cgctcagggc agccgccagc tccggccaga cctcgtcgga gtacgcggcc cggagggcgg
     2821 ccgcccgcag ttcgccggtg ccggccaccc cgcccgccgg agtgcgggcc accgccgccc
     2881 ggacgcgctc gtgactggcc cgcacggcgg ccggggtggc accgaggcgg tacgtggcgt
     2941 ggtgccgggc cgcccaggcg cggaagtcgt accagcggcg ctcgaacccc gccgcctggg
     3001 cgaggttgtc gcggtaccag acgtgccgcg ggtccgggtc gaccgcggag tcgaagacca
     3061 tccggcgcac gtggccgggg tagagcgtgg cgtacaccgc cccgaggtag gtgccgtagg
     3121 aggcgcccac gaaggtcagc tcccgctcgc cgagcgcggc gcgcagcacg tgcaggtccc
     3181 gggcgttgtt gagggtcgtg tagtacggga gggccgcgcc gccccttcgc gcgcagccgc
     3241 gggcgtaggc ccgggcggcg gcgacgcgct ccctcttgta cgcggccgag gggtccgccg
     3301 ggacctgggt gggggccttg gggcggcccg ccgggtcctg gcaggacagc ggggcggagc
     3361 ggccgacgcc gcgcggggcg tagccgacga ggtcgtaggc ggcggcgacg cggcgccagg
     3421 cggggcggtc ggcgagcagc gggaagtaca tgccggaggc gcccggtccg ccggggttga
     3481 agacgaggga gccctggcgg gcggcgcggc cctcgcgggt ggcgccgacc cggctgacgg
     3541 tgagggagat ctgcggtccg tcgggccggg cgtagtcgag cgggacgcgc agggcggcgc
     3601 accggacggg ggcggccagc ccctcggcgg cggggcagct ccggaagtcg agcgcccggt
     3661 gaccggccgc gacgcggcgg gcgacgcgct cggccccggc gttctcggcc gcgaccgcgg
     3721 tggcgggggc cggcgccgga gccggagccg ccggggccgc cggggcgagg gtgagggtca
     3781 gggcgaaggc gagggccgtc gcggcgacgg cggggcgtac gagcggcatc cgcatggcgg
     3841 ccccttcatc tcatcggctg gtccgatgag attccggggc cgcctcccgg ccaaggagca
     3901 ccgccgggtg tcgtccgtga ttgcccccgt tgccgtagcg cggacgggtc aggaactgct
     3961 ggagcagctg gagctcgacg atccgcagct cgaaccaccg ctggagcaac tgctggagga
     4021 ggagccgcag ctggaccccg aagacccgca gccggagccc gacgaaccac agccggagcc
     4081 ggagctcccg ccgcagccgg agctcccgcc gcagccgctg gagccgccgc cggacccgcc
     4141 gtcgacgctc gcgcaccaca cgaccggcag cgcgtcggcg gaggacgacc cccacgagga
     4201 acgggagccc ggggaaccgg cggagcggcg ctgcccggcc tgggccgccg ccagccgggt
     4261 gctgcgggcg gccggcacca gctgctcgcg caggtacggg tcacgcagcc cccgcagccc
     4321 gaacagggcc gtctgcacgt gcggggtctg gtccgtcaga tgggtggccc gcatcgcgcg
     4381 cagcgccgtc cgccccgccg gggtgatccg ccgcttggcc cgcgcggcgg cggcgacgcc
     4441 cacgacgagc ccgccgatca gcacggggaa caccttgacg aggaacggca gctggaaccc
     4501 gggctccagc gcgaacccga cgaaggtcag caccagcgag accggcagca gcgccccgca
     4561 cagcacggcc tgggccagac cccagcgccg ccaccgccgc gcggcgccgg gcgggctgat
     4621 cagcccgcgc tcggcgagcg cgtccccgat ctcctggatc gcggggtcgg tcatggccgc
     4681 gtaccgcacc tggtagagcc agcccgacgg cgcgttcgcg tacgcctgga ggacggcgcg
     4741 ctccgcgacg tccacggccc gcgccccggg ccgcacctgc acgatgcccg ggccgccggc
     4801 caccatccgc tggtcgccca gcagggagac gagcgcggcg tcgaccacgg cgccggggcc
     4861 gcccgccatg aaggcggcct cggacaggtc gagcggtggg tgcgcggggc cgcccggcgc
     4921 gggccgggag cggcgcaggc cgcgcagcag cagcaccgtg gaggtgatga cggcgatcca
     4981 gaccgcccag gcgaggaggt tcatgccgct ctccccacca gggcccgggc ccagcgcacg
     5041 atccggcggg ccgggcgcgt cccggcccgc tcctgccacc aggcggtgag ccggcgccgg
     5101 tcggaggggt cggccggcag ctcccggatc agcaggtgct cggcgaagtc cagcgcgtcg
     5161 cggcggtatc cgccggtcat ggggcggttg cgggcgtacg cgaggaacgc ctcgcggtac
     5221 gccttctcgc cgcccaggat gccggggagc tccggcgcca ccttggccac gaccccggcc
     5281 cgcttggcgg ccagcgaccg cgactgcacc cggatccgct gccggtcgaa gccctcgggc
     5341 accggcgtcc ccgccaccag cgcggacaac agcgcggcct gcgccagccc gacccgggtc
     5401 cgcgcggcgt ccggcacggg gtccgccgcg ggcaccggcg cggcgtccgg tatggggtcc
     5461 ggcgcgggct ccggcgcgga atgccgggaa ggggtgcgga gggaggcgga aggacgggat
     5521 cccgcggggt ccagcacgcc ccggatcgcc tccagttccc cggcgagctc gccctccggc
     5581 gggaagtcgt cgtcccgctc cagcagcacg cccggcgggt ccacccgccg ccgcagctgc
     5641 gcgagtacgt ccagcaccac gcccggcacg gggtgcgcgt gggtgtcgtg ccacacgccg
     5701 tcccgctcga acccgcccgc gacgtgcacg tacgccagcg cctccagcgg gatcgcgtcc
     5761 agtacggcgg ccgggtcctc gccccggttc acccggttcg tgtgcaggtt cgccacgtcg
     5821 atcagcagcc gcaccccggt gcgctcgacc agctccgaca ggaaccggcc ctccgtcatc
     5881 tcctccccgg gccacgagat cagcgcggcg atgttctcca gcgccagcgg caccggcagc
     5941 gcgtcctgcg cgatccgcac gttctcgcac agcacgtcca gcgcgtcccg ggtccgcggc
     6001 accggcagca ggtgccccgc ctccaaggcc ggcgcgggcg tcgtggtccg tacgaacgcg
     6061 atgtgctcgg tgaccagcgg cgcccccagc gccaccgccc gctcgccgag cgcggccagc
     6121 ttctccgggt cgggccggtg cgcgccgccg aggcccagcg agacgccgtg cggtacgacc
     6181 cggacaccgc gctcgcgcag ccgcagcagc gaagccggca gatggcccgg gcagatgttc
     6241 tcggccacga cctcgaccca gtccagcccc ggcagccgct ccacggcgtc cgcgatctcc
     6301 ggccgccagc cgatgcccac ccccaggtgt gccatgggct tcatgtcccc tcgcccccct
     6361 catcggtccg tgtgcccatg tcatcgccac ggccggggcg ggccaaccac cgccgccgga
     6421 cgttcagagg aacatttgag gttccggagg tcccgccctc acagccgggc ccgcagcgcc
     6481 ggatggtccg agaccaccgt ggcgctgccg ggcgcgatct ccgtgaaacc ggcgtcccgt
     6541 acgaccggca gaccgctccc gctcagccgc gcccaccgct cgcgcggggc cgtccgtacg
     6601 gccagccgga aaccggaatc ctgccaggcg gtccgctccg cggcggtcag ctcccaccac
     6661 gccagctggg ccgcgtgccc ggcctgggcc atcgccttgc cggcggacat ctccagatcc
     6721 gggttcagcc acagcaccgc gatcccgtcc ggcgccggag cgaccggctc cgggtcgtcg
     6781 aggtcggtcc ccgagacctg gagcttggcc agttccttcg gccacccgtc gagcgggacc
     6841 ggcgggaaca cccgtacctc cgccgcgtca ccgtgcaccg tgacacccgg cagggtctcg
     6901 gccttgcgcc actccgcgcc gcgcgcccgc cggaccacct tgcggatccg cgcgtcctgc
     6961 cagtcgcgga ccgccccggc ccattcgccc tcgccgagcg cccgctcgtc gctcagcagc
     7021 accagcaccg cgcgggccgc cgtctccagc gcgtcggtac gggccggggg ttcggccttc
     7081 tcgatccgga cgaccagggg gagcacgaac tgcggtgcct cgtcgcgggc ggaccgctgc
     7141 ggccgcggcg ggtgctccgc gcccgcggcg ggtaccgggg tgctgtcgtc gtggtgcgtg
     7201 tccgggctgc tcatcgcgct gttcatcccc tgctcatgcc gctgttcatc ctcgcaaagg
     7261 atgccagcct cccccttcag gatgatgccg agggggttcg ggccgggcga ggatcgaacc
     7321 catgaagagt gatcttttcg cgtccgagca cctcgcccag ccgtccgccg ccccgggcat
     7381 gaccctgcag aacgccaagt ccgtgaagta caccgtcaac ggtgaaatgc tggcccgtca
     7441 gggctcgatg gtcgccttcc gcggcaacct ccagttcgag cgcaagggcc agggcatagg
     7501 cggcatgctc aagcgcgccg tcaccggcga gggcctggcg ctgatgtccg tacgcgggca
     7561 gggcgacgca tggttcgcgc accaggccgg caactgcttc atcatcgact tcgagcccgg
     7621 cgacgccctg accatcaacg gccgcaacgt gctctgcttc gacccgaccc tgtcctacga
     7681 gataaagatg gtgaagggcg ccggcatgac cggcgggggc ctcttcaaca gcgtcttcac
     7741 cggcaccggc aagctcgccg tggtctgcga cggccacccg atcatcatcc cggtcaccgc
     7801 ccagaacccg gtctacgtgg acacggacgc ggtcgtcggc tggagcgccc agctggagac
     7861 gggcctgcac cgctcgcagt ccgtcggctc gatgatccgc ggcggctcgg gcgaggccgt
     7921 ccagctgatg ctgcgcggcg agggcttcgt catcgtccgc ccgagcgagc tgacggaaac
     7981 cgcggccgcg cactgaacag gaccccaccg aagacgacgg ccgtgcgacg ggacgccatg
     8041 cgactggacg ccgtgggacg ccgctacggc gtgcggggac cctgggtgct gcgcggggtc
     8101 tcgctggagc tgccgcccgg cgcgctcgtc cgcgtcgaag gacgcaacgg cggcggcaag
     8161 tccacgctgc tgcggctcgt ggccggcatc gacaccccga ccgagggaag catcaccggc
     8221 cgcccgcgcc gcaccgcata cgtccccgag cgcttcccgg ccgccctgcc gctcaccgcg
     8281 caggcctacc tcggccacct gggccgggtc cacgggctga cgcgcgccga ggccgcgcgc
     8341 cgggcggcgc agtggctgga gcggttcggc gcggccgggc acgcccgcac cccgctggcc
     8401 gaactgtcca aggggaccag ccagaaggtg gccgtcgccc aggccctgct cgccggaccg
     8461 gggctgctcg tcctcgacga ggcgtggacc gggctcgacg tgcccgcgcg cgccgagctg
     8521 gaccgggccg tggccgagcg gaccgccgcc gggggcacgg tgctgttcgt ggaccacgac
     8581 ccggcgcggc tcgccggggc acccgaccgc cactaccggg tcgagggggg cgcgttggaa
     8641 cccgtacggc cgggcgccgg gcccgggccc cgcgtgcgga tcgaggccgc cggaccgccc
     8701 ggcgccgtcc cgccccccgg cctggcggcc gagcccctgc ccgccggcgg attccgcctg
     8761 accgtgcccg ccggccggtc cgacgcggtg ctgcgcgaac tgctcaccgc acaccctgcc
     8821 tggcacatcc ggaacgtgag tgacctgccg tgaccgctct gctgcgctat cagggcgcgc
     8881 tgttgctgcg ctcgcagcgc tggctcgcgc cggtgctgct gtacgccgcc ttcctcgccg
     8941 tcgggttccg ccccggggat cccgtaccgg actcgctggg ctactcggcc gccgcgctgg
     9001 tcccggtgac ggcctggctg gtgcggacct gcctctcggt cgaacccgac gcggcgcggg
     9061 actgcgcggc cgccgcggcg ggcccggtac gggtgcacgc ggcggccctg ctcaccgggc
     9121 tgggctgggg cgccggcgcg gggggcctcg gcgccgtcgc ggtgctgctg ctcggcgccc
     9181 gcgaggggcc gcggctcccg accgccgccc tggcgggggc cgcgtcggtg ctggtgtgcg
     9241 cgctgacggg cgcggccgtg ggcgcgctgt gcgcccggcc gctgctgagg gcccccgggt
     9301 acgcggtcgc ggcggcgggc ctcggcagcc tgctggccct ggtcacggcg ggctcgccgg
     9361 cccgggtggc ggtcagcggg ctggtggggg gctcggtggc gctcccgctg ggagcgctgg
     9421 cgggcgcggc ggcgctggcc tgcgcggcgg gcgcggccgc ctgcctggcc gccgcgcggc
     9481 gggggtgagt acgctcgggg gcatggacca gtgcgagccg accgagtcgt actgccccgc
     9541 gccggcggcg cccgcccgcg tcgtggcggg accgccgtac gcggactgcc tggagtgcgg
     9601 caagcccacc gagtacgggg tccggacccc cggcgtggtg ctgtgcccgg tctgcgagtg
     9661 gcaggaagcc cagcgcaccg cctgctcggg ctagggcccg atcccaaaga caggccctag
     9721 gtgcccatga gctcggagac cttcacgaag cggtagccgc gcttgcgcag ctccgggacg
     9781 atcgcgcgga tggcctcctc ggtgacgggc gccgcgctgc gggtgcagtg catgaccacc
     9841 agcgagcccg gcttcacccc ggccaggacc tgctcggcca ccgcgtccgg gtccttcgcg
     9901 aaggcgtccc cgctgaccac gtcccactgc accgccgtga ccttggccgt ggacagggcc
     9961 cgcagcgcct ggtcgtcgta gcagccgccg gggaagcgga agtacgggac ggtgttgacc
    10021 gcgcccgcct tgcggaaggc gtcgaaggcc cggtccacgt cggcccgggc ggcggcgccg
    10081 tcgagcgtgg ggagcccgta gcagggggac ttgaaggcgt ggtggctgta cgagtggttg
    10141 gcgatctcga agttcgggtc ggtgccgatg gctttggcct ggtccgggta ctcctcggcc
    10201 cagcggcccg tcatgaagac cgtcgagggc accttgagcg cccgcagcgt gtcgatcagc
    10261 tgggggttgt cgaagtgctc cccgcccgcc gcgcgcggtc cctgatcgga ggtcatgtcc
    10321 gcgtcgaagg tcagggcgac ggtcttctcg gtcttctgcc tggcccgctc gaagaccggg
    10381 gtcaggccgc ccgggccggg ggccagcgtc ggggccttgc cgggggcgcc ggccctcggg
    10441 agcgcggagg ccgctccggc ggcgggggcg ggtgcggagg cccgggccga cgcggaggcg
    10501 gaggccgagg gggtggcgga cgcgccgagc cgggcctggc cggcgccgcc gttcgaggga
    10561 ccggcgccgc ccccgcagcc ggcgaggacg gcgccgaggg cgccactgag gacggccgcg
    10621 gccgccgctt tgcgcaggga agaggtcacc tacgcaaaat atatgactat gtggcaatca
    10681 gacgggcacg gcactcctcc acgtcgaacc cggcctccgg gtaccgcgga ccgatgagct
    10741 ccaggtgctc ccgcagcagg gtggcgacgg cccagtcgcg gtaccacttg cggtccgcgg
    10801 ggacgacgaa ccacggcgcg gcgtccgtgg agcaccgctc cagcgccagc tcgtaggcct
    10861 cctggtacgc gggccacagc gcccgctcgt cgatgtcgga cggggcgaac ttccagtgct
    10921 tggaagggtc gtccagcctt cgcaggagcc gctcgcgctg cttctcgtag ctgatgtgga
    10981 ggaagacctt caccacggtc accccgtccc gcgcgaggga ctcctcgaac cggttgatct
    11041 gcgcgtagcg gtggttcacc tcgctgcggg acaccagttc gcggacccgg gcgatcagca
    11101 cgtcctcgta gtgcgagcgg tcgaagacgc cgatctcgcc cggcttcggg agggcgcggg
    11161 cgatccgcca caggaagggg tgcgaccgct cctcgtccgt gggcgccttg aaggccctga
    11221 tccggcagcc cgcggggttg agcgggccgg tcacgcgctt gaccgcgccg cccttgccgc
    11281 tggtgtccat gccctggagg acgaggagca cccggcggcg gtcgcccgcg gtgctcgcgg
    11341 cgaa
//
