LOCUS       NZ_BMUU01000046         2633 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Streptomyces xanthochromogenes strain JCM 4594 sequence46, whole
            genome shotgun sequence.
ACCESSION   NZ_BMUU01000046
VERSION     NZ_BMUU01000046.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces xanthochromogenes
  ORGANISM  Streptomyces xanthochromogenes
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 2633)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650355.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:28:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,827
            CDSs (total)                      :: 7,752
            Genes (coding)                    :: 7,642
            CDSs (with protein)               :: 7,642
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 24 of 110
            Pseudo Genes (incomplete)         :: 94 of 110
            Pseudo Genes (internal stop)      :: 7 of 110
            Pseudo Genes (multiple problems)  :: 12 of 110
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:05:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2633
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUU01000046.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2633
                     /culture_collection="JCM:4594"
                     /db_xref="taxon:67384"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces xanthochromogenes"
                     /strain="JCM 4594"
                     /submitter_seqid="sequence46"
                     /type_material="type strain of Streptomyces michiganensis"
     gene            <1..>2633
                     /db_xref="GeneID:96295723"
                     /locus_tag="IE233_RS38740"
     region          1..2633
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2633
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2633
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[0:>2633](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..>2633
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             1..>2633
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (81-149). E-value: 2.2e-27. Score:
                     87.0. Matches aSDomain: nrpspksdomains_IE233_RS38740_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (166-461). E-value:
                     1.3e-113. Score: 370.9. Matches aSDomain:
                     nrpspksdomains_IE233_RS38740_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (631-872). E-value: 1.8e-61.
                     Score: 199.9. Matches aSDomain:
                     nrpspksdomains_IE233_RS38740_AMP-binding.1"
                     /codon_start=1
                     /db_xref="GeneID:96295723"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IE233_RS38740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_190029705.1"
                     /sec_met_domain="PP-binding (E-value: 2.4e-20, bitscore:
                     59.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2.1e-74, bitscore:
                     236.3, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 2.9e-55, bitscore:
                     173.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAVVVREDRPGDKRLVAYVVSEADADTLHGHCADRLAEYLVPSAF
                     VALAELPLTANGKLDRAALPAPQAGGATGRAPRTPAEEVLCGLFADILGVPAVSVDDDF
                     FRLGGHSLLATRLVGRVRSALATELTLRDFFQYPTVARLAEHIAGGAGTAPRPQLTVVP
                     RSDEHLPLSAAQRRLWFLDQMEGPSSTYNIPLAVTLTGAVDPELLRQAIGDVLARHEVL
                     RTSYPVHDGEPRQHIAHPDEVTVPLAVADVTEDELADRIAGEAAHLFDLAAELPLHAAL
                     LRTGADRSVLVLVVHHIASDGWSNTPLMRDLGTAYTARAEGTAPGWEPLPVQYADYALW
                     QRDLLAEHEGTQLDHWRTALAGLPAESTLPADRPRPAVASYRGSTHTVHCPGETHRALT
                     ALARESGATLFMVAQAATVALLSRSGAGTDIPVGSLVAGRTDGALEDLVGFFVNTLVLR
                     TDASGDPTFRDLLDRVRETDLGAWAHQDLPFDRLVEALNPERSAARHPLFQVILTLAEA
                     AEATPALPGVRARTGQPGMGTAKFDLTVNFYEHRDRTGGPNGLDIVLEYATDLYDPATI
                     ETAADRLTRVLDAVIADPDVRVADIELLTRDQRRALLTDYNDTAATLPTGTLHELFAAQ
                     AARTPDAPALVCGESELSYRALDRASDAFARRLAGLGVVPGTAVGLFLDRSPEYVVAAL
                     GVLKAGGVYVPLDARQPRERLAFILADTGATLLVTDRDAPADGLTPLSPPDVPALLAEA
                     EAQTETFQLPLHADQLAYVMYTSGSSGTPKGVANTHRNVVELALDPWWGAGERHRRVLA
                     YSPLAFDSSTYELWVPLLSGGTAVILPVSKVDVAELGEALVRHGITAVYFTTALFDAMA
                     SEAVGA"
     aSDomain        244..447
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38740_PCP.1"
                     /evalue="2.20E-27"
                     /label="IE233_RS38740_PCP.1"
                     /locus_tag="IE233_RS38740"
                     /protein_end="149"
                     /protein_start="81"
                     /score="87.0"
                     /tool="antismash"
                     /translation="EEVLCGLFADILGVPAVSVDDDFFRLGGHSLLATRLVGRVRSALA
                     TELTLRDFFQYPTVARLAEHIAG"
     aSDomain        499..1383
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38740_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.30E-113"
                     /label="IE233_RS38740_Condensation_LCL.1"
                     /locus_tag="IE233_RS38740"
                     /protein_end="461"
                     /protein_start="166"
                     /score="370.9"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIASDG"
                     /tool="antismash"
                     /translation="EHLPLSAAQRRLWFLDQMEGPSSTYNIPLAVTLTGAVDPELLRQA
                     IGDVLARHEVLRTSYPVHDGEPRQHIAHPDEVTVPLAVADVTEDELADRIAGEAAHLFD
                     LAAELPLHAALLRTGADRSVLVLVVHHIASDGWSNTPLMRDLGTAYTARAEGTAPGWEP
                     LPVQYADYALWQRDLLAEHEGTQLDHWRTALAGLPAESTLPADRPRPAVASYRGSTHTV
                     HCPGETHRALTALARESGATLFMVAQAATVALLSRSGAGTDIPVGSLVAGRTDGALEDL
                     VGFFVNTLVLRTDA"
     aSModule        499..2616
                     /domains="nrpspksdomains_IE233_RS38740_Condensation_LCL.1"
                     /domains="nrpspksdomains_IE233_RS38740_AMP-binding.1"
                     /incomplete
                     /locus_tags="IE233_RS38740"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       568..681
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38740_0001"
                     /evalue="1.50E-17"
                     /label="C2_LCL_024-062"
                     /locus_tag="IE233_RS38740"
                     /protein_end="227"
                     /protein_start="189"
                     /score="56.0"
                     /tool="antismash"
                     /translation="TYNIPLAVTLTGAVDPELLRQAIGDVLARHEVLRTSYP"
     CDS_motif       883..948
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38740_0002"
                     /evalue="9.40E-11"
                     /label="C3_LCL_132-143"
                     /locus_tag="IE233_RS38740"
                     /protein_end="316"
                     /protein_start="294"
                     /score="34.1"
                     /tool="antismash"
                     /translation="VHHIASDGWSNTPLMRDLGTAY"
     CDS_motif       997..1035
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38740_0003"
                     /evalue="5.50E-06"
                     /label="C4_LCL_164-176"
                     /locus_tag="IE233_RS38740"
                     /protein_end="345"
                     /protein_start="332"
                     /score="18.8"
                     /tool="antismash"
                     /translation="QYADYALWQRDLL"
     CDS_motif       1285..1374
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38740_0004"
                     /evalue="1.70E-14"
                     /label="C5_LCL_267-296"
                     /locus_tag="IE233_RS38740"
                     /protein_end="458"
                     /protein_start="428"
                     /score="46.1"
                     /tool="antismash"
                     /translation="DIPVGSLVAGRTDGALEDLVGFFVNTLVLR"
     CDS_motif       1423..1542
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38740_0005"
                     /evalue="7.20E-23"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="IE233_RS38740"
                     /protein_end="514"
                     /protein_start="474"
                     /score="73.1"
                     /tool="antismash"
                     /translation="RETDLGAWAHQDLPFDRLVEALNPERSAARHPLFQVILTL"
     aSDomain        1894..2616
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38740_AMP-binding.1"
                     /evalue="1.80E-61"
                     /label="IE233_RS38740_AMP-binding.1"
                     /locus_tag="IE233_RS38740"
                     /protein_end="872"
                     /protein_start="631"
                     /score="199.9"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FAAQAARTPDAPALVCGESELSYRALDRASDAFARRLAGLGVVPG
                     TAVGLFLDRSPEYVVAALGVLKAGGVYVPLDARQPRERLAFILADTGATLLVTDRDAPA
                     DGLTPLSPPDVPALLAEAEAQTETFQLPLHADQLAYVMYTSGSSGTPKGVANTHRNVVE
                     LALDPWWGAGERHRRVLAYSPLAFDSSTYELWVPLLSGGTAVILPVSKVDVAELGEALV
                     RHGITAVYFTTALFDAMAS"
     CDS_motif       2080..2121
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38740_0006"
                     /evalue="8.10E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="IE233_RS38740"
                     /protein_end="707"
                     /protein_start="693"
                     /score="18.9"
                     /tool="antismash"
                     /translation="LGVLKAGGVYVPLD"
     CDS_motif       2308..2367
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38740_0007"
                     /evalue="3.00E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="IE233_RS38740"
                     /protein_end="789"
                     /protein_start="769"
                     /score="28.9"
                     /tool="antismash"
                     /translation="AYVMYTSGSSGTPKGVANTH"
ORIGIN
        1 gcggcggtgg tcgtgcgtga ggaccgtccc ggcgacaagc gcctcgtcgc ctacgtcgta
       61 tcggaggccg acgccgacac cctgcacggg cactgcgcgg accgcctcgc cgaatacctg
      121 gtgccctccg ccttcgtcgc actcgccgaa ctgccgctga ccgccaacgg caagctcgac
      181 cgcgcagccc tgcccgcacc gcaggccggc ggcgccacgg gccgggcccc gcgcacaccc
      241 gccgaagagg tgctgtgcgg actgttcgcc gacatactcg gcgtgcccgc ggtctccgtc
      301 gacgacgact tcttccgcct cggcgggcac tctctgctcg ccacccgcct ggtcggccgc
      361 gtgcgcagcg ccctcgcgac ggaactgacc ctgcgggact tcttccagta cccgaccgtc
      421 gcccggctcg ccgagcacat cgccggcggc gccgggacgg ccccgcgccc ccagctcacc
      481 gtcgtcccgc gctcggacga gcacctgccg ctgtcggcgg cacagcggcg gctgtggttc
      541 ctggaccaga tggagggccc ctccagcacc tacaacatcc cgctcgccgt caccctcacc
      601 ggcgccgtcg accccgagct gctgcggcag gccatcggtg acgtgctcgc ccggcacgag
      661 gtgctgcgca ccagctaccc ggtgcacgac ggcgagcccc ggcagcacat cgcccacccc
      721 gacgaggtga ccgtcccgct ggccgtcgcc gacgtcaccg aggacgagct ggccgaccgg
      781 atcgccgggg aagccgcgca cctcttcgac ctcgcggccg aactaccgct gcacgcggcc
      841 ctgttgagga ccggcgccga ccggagcgtg ctggtcctgg tcgtccacca catcgcctcc
      901 gacggctggt ccaacacccc gctgatgcgg gacctcggca ccgcctacac ggcccgcgcc
      961 gagggcacgg cccccggctg ggagccgctg ccggtccagt acgccgacta cgcgctgtgg
     1021 cagcgggacc tgctcgccga gcacgagggg acgcagctcg accactggcg caccgcactc
     1081 gccgggctgc ccgccgagtc gaccctgccc gccgaccggc cccgccccgc cgtcgcctcc
     1141 taccggggca gcacccacac cgtgcactgc ccgggcgaga cccaccgggc gctcaccgcg
     1201 ctcgccaggg agtccggcgc cacgctcttc atggtggcgc aggccgccac ggtcgccctg
     1261 ctctcccgct ccggggccgg gaccgacatc ccggtcggct ccctggtcgc gggccgcacc
     1321 gacggggccc ttgaggacct ggtcggcttc ttcgtcaaca cgctggtcct gcgcaccgac
     1381 gcgagcggcg accccacctt ccgcgacctc ctcgaccggg tgcgggagac cgacctgggc
     1441 gcctgggcac accaggacct gcccttcgac cggctggtgg aggccctcaa cccggagcgg
     1501 tccgccgccc ggcaccccct cttccaggtc atactcaccc tggccgaggc cgccgaggcc
     1561 acccccgcgc tgcccggggt gcgcgccagg acgggccaac ccggcatggg caccgccaag
     1621 ttcgacctca ccgtcaactt ctacgagcac cgcgaccgga cgggcggccc gaacgggctc
     1681 gacatcgtcc tggagtacgc caccgacctg tacgaccccg cgacgatcga gaccgccgcc
     1741 gaccggctga cccgggtcct ggacgccgtg atcgccgacc ccgacgtccg cgtggccgac
     1801 atcgagctgc tcacccgcga ccagcgccgc gccctgctca ccgactacaa cgacaccgcc
     1861 gccaccctgc ccaccggcac cctgcacgaa ctgttcgccg cgcaggccgc ccgcaccccc
     1921 gacgcgcccg cgctggtgtg cggggagagc gagctgtcgt accgcgcact ggaccgcgcg
     1981 tccgatgcct tcgcccgccg cctggccggc ctcggcgtcg tccccggcac cgccgtgggt
     2041 ctcttcctcg accggtcacc ggagtacgtg gtcgccgccc tgggcgtcct caaggcgggt
     2101 ggcgtgtacg tgccgctgga cgcccgtcag ccccgggagc gcctggcgtt catcctggcc
     2161 gacaccggcg ccaccctcct cgtcaccgac cgggacgccc cggccgacgg cctgaccccg
     2221 ctgtccccgc cggacgtccc ggccctgctg gccgaggccg aagcgcagac cgagaccttc
     2281 caacttccgt tgcatgcgga ccagttggcg tatgtgatgt atacgtcggg gtcgagtggg
     2341 acgccgaagg gtgtggcgaa tacgcatcgc aatgtggtgg agctggcgtt ggacccgtgg
     2401 tggggtgcgg gggagcggca tcggcgggtg ctggcgtatt cgccgttggc cttcgactcc
     2461 tcgacgtacg agctgtgggt gccgctcctc agcggcggca ccgcggtgat ccttccggtt
     2521 tccaaggtcg atgtggccga gctcggtgag gctctcgtcc gccatggcat cactgccgtg
     2581 tatttcacga cggcgttgtt cgatgcgatg gcgtcggagg cggtgggggc gtt
//
