LOCUS       NZ_BMUU01000045         2681 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Streptomyces xanthochromogenes strain JCM 4594 sequence45, whole
            genome shotgun sequence.
ACCESSION   NZ_BMUU01000045
VERSION     NZ_BMUU01000045.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces xanthochromogenes
  ORGANISM  Streptomyces xanthochromogenes
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650355.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:28:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,827
            CDSs (total)                      :: 7,752
            Genes (coding)                    :: 7,642
            CDSs (with protein)               :: 7,642
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 24 of 110
            Pseudo Genes (incomplete)         :: 94 of 110
            Pseudo Genes (internal stop)      :: 7 of 110
            Pseudo Genes (multiple problems)  :: 12 of 110
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:05:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2681
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUU01000045.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2681
                     /culture_collection="JCM:4594"
                     /db_xref="taxon:67384"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces xanthochromogenes"
                     /strain="JCM 4594"
                     /submitter_seqid="sequence45"
                     /type_material="type strain of Streptomyces michiganensis"
     gene            <1..>2681
                     /db_xref="GeneID:96295722"
                     /locus_tag="IE233_RS38735"
     region          1..2681
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..2681
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2681
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[4:>2681](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5..>2681
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             5..>2681
                     /NRPS_PKS="Domain: PCP (1-45). E-value: 6.9e-15. Score:
                     47.0. Matches aSDomain: nrpspksdomains_IE233_RS38735_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (63-363). E-value:
                     1.1e-108. Score: 354.7. Matches aSDomain:
                     nrpspksdomains_IE233_RS38735_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (536-892). E-value: 3.9e-88.
                     Score: 287.7. Matches aSDomain:
                     nrpspksdomains_IE233_RS38735_AMP-binding.1"
                     /codon_start=3
                     /db_xref="GeneID:96295722"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IE233_RS38735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_190029704.1"
                     /sec_met_domain="PP-binding (E-value: 3.8e-14, bitscore:
                     39.3, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2.6e-64, bitscore:
                     203.2, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 9.2e-85, bitscore:
                     270.7, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFELGGHSLSATRLVSRIRSVFGVDVTVREVFSCPSVARMAALVA
                     VGESAARPALAPVVPRPELLPLSFAQQRLWVLAQLDGGSATYNIPMAVRLRGGVDRVAL
                     AAALIDLVGRHESLRTVFPVGENGPVQRVLAPAEVDVDLRVVETSEAESEAVLRGLAWY
                     AFDLAQEVPVRATLVETAPDDCVLSLVVHHIASDGWSNTPLLRDLGTAYTARSAGRAPD
                     WAPLPVQYADYALWQRELLGDAADPSSPMSRQTGFWREALADLPLEATLPGDRPRPAVA
                     TYQGGRVDLRIDATVHERLVRLCRTCDTSLFMAVQAATAAVLTLSGAGTDVPLGSPVAG
                     RHDVVLDDLVGFFINTLVLRTDTSGDPSFRQLLARVREADLAAWAHQDLPFERLVEVLS
                     PERSASRHPLFQTMLTFADAVIPGPAMPGLISDVAETPAGAARFDLTVNFREYRLEGGR
                     PGGLDLGIDYAAEIFDETTVRAFGERLVRLVAGVVETPDRSIGDIDVLTPGERAWLSGA
                     GSGELGARAVSSLPELVRAYADDRPDAAAVVCGERVLTYAEFEEQANRLARVLVTAGVG
                     PESRVVLFQDRGVEVLVSMLAVLKAGGAYVPLDTRYPRARIEEILRQASASMVLIGRDV
                     SAAELPEGASVLRVPPLERWRPGDAVTPPPDVRVHPDQLAYVMFTSGSTGVPKGSANTH
                     RNIVELARDEVFGNGVAERMLQYSSLAFDASTIEIWGPLSRGGCVEVAPPGALDSTQLG
                     RLLTERKVPALFLPAGLFHVLAEENPEAFREVREVWAGGDVVSPEAVRRVLAHCPDITV
                     HNGYGPTETTVFVTVHRVDKTMADARALPIGTPLAHTGVYILDEALRLVPPGVVGELYV
                     AGAHLARGYVGRAGLTAERFVA"
     aSDomain        10..141
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38735_PCP.1"
                     /evalue="6.90E-15"
                     /label="IE233_RS38735_PCP.1"
                     /locus_tag="IE233_RS38735"
                     /protein_end="45"
                     /protein_start="1"
                     /score="47.0"
                     /tool="antismash"
                     /translation="FELGGHSLSATRLVSRIRSVFGVDVTVREVFSCPSVARMAALVA"
     aSDomain        196..1095
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38735_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.10E-108"
                     /label="IE233_RS38735_Condensation_LCL.1"
                     /locus_tag="IE233_RS38735"
                     /protein_end="363"
                     /protein_start="63"
                     /score="354.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIASDG"
                     /tool="antismash"
                     /translation="LLPLSFAQQRLWVLAQLDGGSATYNIPMAVRLRGGVDRVALAAAL
                     IDLVGRHESLRTVFPVGENGPVQRVLAPAEVDVDLRVVETSEAESEAVLRGLAWYAFDL
                     AQEVPVRATLVETAPDDCVLSLVVHHIASDGWSNTPLLRDLGTAYTARSAGRAPDWAPL
                     PVQYADYALWQRELLGDAADPSSPMSRQTGFWREALADLPLEATLPGDRPRPAVATYQG
                     GRVDLRIDATVHERLVRLCRTCDTSLFMAVQAATAAVLTLSGAGTDVPLGSPVAGRHDV
                     VLDDLVGFFINTLVLRTDT"
     aSModule        196..>2681
                     /domains="nrpspksdomains_IE233_RS38735_Condensation_LCL.1"
                     /domains="nrpspksdomains_IE233_RS38735_AMP-binding.1"
                     /incomplete
                     /locus_tags="IE233_RS38735"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       259..375
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38735_0001"
                     /evalue="9.20E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="IE233_RS38735"
                     /protein_end="123"
                     /protein_start="84"
                     /score="56.6"
                     /tool="antismash"
                     /translation="ATYNIPMAVRLRGGVDRVALAAALIDLVGRHESLRTVFP"
     CDS_motif       577..642
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38735_0002"
                     /evalue="1.60E-10"
                     /label="C3_LCL_132-143"
                     /locus_tag="IE233_RS38735"
                     /protein_end="212"
                     /protein_start="190"
                     /score="33.4"
                     /tool="antismash"
                     /translation="VHHIASDGWSNTPLLRDLGTAY"
     CDS_motif       691..729
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38735_0003"
                     /evalue="1.50E-06"
                     /label="C4_LCL_164-176"
                     /locus_tag="IE233_RS38735"
                     /protein_end="241"
                     /protein_start="228"
                     /score="20.5"
                     /tool="antismash"
                     /translation="QYADYALWQRELL"
     CDS_motif       997..1086
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38735_0004"
                     /evalue="3.00E-13"
                     /label="C5_LCL_267-296"
                     /locus_tag="IE233_RS38735"
                     /protein_end="360"
                     /protein_start="330"
                     /score="42.1"
                     /tool="antismash"
                     /translation="DVPLGSPVAGRHDVVLDDLVGFFINTLVLR"
     CDS_motif       1135..1254
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38735_0005"
                     /evalue="2.40E-22"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="IE233_RS38735"
                     /protein_end="416"
                     /protein_start="376"
                     /score="71.4"
                     /tool="antismash"
                     /translation="READLAAWAHQDLPFERLVEVLSPERSASRHPLFQTMLTF"
     aSDomain        1615..>2681
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38735_AMP-binding.1"
                     /evalue="3.90E-88"
                     /label="IE233_RS38735_AMP-binding.1"
                     /locus_tag="IE233_RS38735"
                     /protein_end="892"
                     /protein_start="536"
                     /score="287.7"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="YADDRPDAAAVVCGERVLTYAEFEEQANRLARVLVTAGVGPESRV
                     VLFQDRGVEVLVSMLAVLKAGGAYVPLDTRYPRARIEEILRQASASMVLIGRDVSAAEL
                     PEGASVLRVPPLERWRPGDAVTPPPDVRVHPDQLAYVMFTSGSTGVPKGSANTHRNIVE
                     LARDEVFGNGVAERMLQYSSLAFDASTIEIWGPLSRGGCVEVAPPGALDSTQLGRLLTE
                     RKVPALFLPAGLFHVLAEENPEAFREVREVWAGGDVVSPEAVRRVLAHCPDITVHNGYG
                     PTETTVFVTVHRVDKTMADARALPIGTPLAHTGVYILDEALRLVPPGVVGELYVAGAHL
                     ARGYVGRAGLTAERFV"
     CDS_motif       1792..1833
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38735_0006"
                     /evalue="1.60E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="IE233_RS38735"
                     /protein_end="609"
                     /protein_start="595"
                     /score="21.0"
                     /tool="antismash"
                     /translation="LAVLKAGGAYVPLD"
     CDS_motif       1885..1920
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38735_0007"
                     /evalue="2.80E+01"
                     /label="C3_LCL_132-143"
                     /locus_tag="IE233_RS38735"
                     /protein_end="638"
                     /protein_start="626"
                     /score="-2.2"
                     /tool="antismash"
                     /translation="SMVLIGRDVSAA"
     CDS_motif       2029..2088
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38735_0008"
                     /evalue="1.70E-08"
                     /label="NRPS-A_a3"
                     /locus_tag="IE233_RS38735"
                     /protein_end="694"
                     /protein_start="674"
                     /score="26.6"
                     /tool="antismash"
                     /translation="AYVMFTSGSTGVPKGSANTH"
     misc_feature    2447..2449
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS_motif       2590..2679
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38735_0009"
                     /evalue="1.40E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="IE233_RS38735"
                     /protein_end="891"
                     /protein_start="861"
                     /score="49.4"
                     /tool="antismash"
                     /translation="PPGVVGELYVAGAHLARGYVGRAGLTAERF"
     misc_feature    2609..2611
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    2639..2641
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
ORIGIN
        1 atttcttcga actcggcggt cattccctgt ccgcgacgag actggtcagc cggatacggt
       61 cggtgttcgg tgtcgatgtg acggtgcggg aggtcttcag ttgcccgtcg gtcgcccgga
      121 tggcggctct ggtcgcggtg ggcgagtcgg ctgcccggcc ggcgctcgcg ccggtggtgc
      181 cccgcccgga gctcctgccg ttgtcgttcg cacagcaacg gctgtgggtc ctggcgcagt
      241 tggacggcgg ctcggcgacc tacaacattc cgatggcggt ccggctgcgt gggggcgtgg
      301 accgggtggc gctggcggct gcactgatcg atctcgtcgg gcgccacgag agcctgcgga
      361 ccgtgtttcc ggtgggtgag aacggcccgg tccagcgggt gctcgcgcct gccgaggtgg
      421 atgtggatct gcgtgtcgtg gagacttccg aggccgagtc ggaggcggtg ctgcgaggtt
      481 tggcgtggta tgcgttcgac ctggcacagg aggtgccggt ccgggccact ctggtggaga
      541 cggctccgga cgactgtgtg ctgtcgttgg tggtgcacca catcgcttct gacgggtggt
      601 cgaacacgcc gctgctacgg gacttgggga cggcgtacac ggcccgctcg gcggggcggg
      661 cgcccgattg ggcgccgctg ccggtgcagt acgccgacta tgcgttgtgg caaagggagt
      721 tgctcggcga cgccgcagat cccagcagtc cgatgtcgcg gcagacgggc ttctggcggg
      781 aggcactggc cgatctgccg ctggaagcga cgctgcccgg cgaccggccc cggcctgcgg
      841 tggcgaccta tcagggcggc cgcgtcgacc tgcgcatcga tgccaccgtt cacgagcggc
      901 tggtgcggtt gtgccggact tgcgacacct cgttgttcat ggccgtccaa gcggccaccg
      961 ccgcggtgct caccctcagc ggtgccggga cggacgtgcc gctcggctcg ccggtcgccg
     1021 gtcggcacga cgtcgtcctg gacgatctgg tcgggttctt catcaacacg ctggtcctgc
     1081 gcaccgacac cagcggggac cccagcttcc ggcagctgct ggcccgggtc cgggaagccg
     1141 atctggccgc ctgggcccat caggacctgc cgttcgagcg cctcgtcgaa gtactgagcc
     1201 cggaacgctc cgcctcccgc caccccctct tccagaccat gctcaccttc gcggacgcgg
     1261 tcatccccgg gccggcgatg ccgggcctga tctcggacgt ggcggagacc cctgcgggcg
     1321 cggcccgctt cgatctgacg gtcaacttcc gggagtaccg gctggagggg ggccggcccg
     1381 gtggtctgga tctgggcatc gactacgccg ccgagatctt cgacgaaacc accgttcgtg
     1441 cgttcggtga acggctcgta cggttggtgg ccggtgtggt ggagacgccg gatcggtcga
     1501 tcggcgacat cgatgtgctc acccccggcg agcgggcgtg gctgtcgggg gcgggcagcg
     1561 gtgagctggg ggcccgggcg gtgtcgtcct tgccggaact cgtgcgcgcc tacgccgatg
     1621 accggccgga tgcggccgcc gttgtgtgcg gtgagcgcgt tttgacctac gcggagttcg
     1681 aggagcaggc gaaccgtctg gctcgggtgc ttgtcacggc cggagtcggt cccgaatccc
     1741 gggtcgtcct gttccaggac cgaggcgtcg aggtgctggt gtcgatgctg gcggtgctga
     1801 aggccggcgg ggcctatgtc ccgttggaca cccggtatcc cagggcccgg atcgaggaga
     1861 tcctgcggca ggcatcggcg tcgatggtgc tgatcggccg ggatgtgtcc gccgcggagc
     1921 tgccggaggg ggcgtcggtg ttgcgggtgc cgccgttgga gcgctggcgg ccgggcgatg
     1981 cggtgacgcc gcccccggac gtgcgggtgc atcccgatca gctcgcctac gtgatgttca
     2041 cctccggttc gacaggagtg cccaaggggt cggccaacac ccaccgcaac atcgtggagc
     2101 tggcgcgtga cgaggtgttc ggcaacggtg tcgccgaacg catgctgcag tactcgtcgt
     2161 tggccttcga cgcttcgacc atcgagatat ggggtccgct gtcgcggggc ggttgtgtgg
     2221 aggtggcgcc gcccggggca ctggactcga cgcagttggg ccgactgctg accgaacgca
     2281 aggtgccggc gctgttcttg cccgcgggcc tgttccacgt gctcgccgag gagaacccgg
     2341 aggcgttccg cgaagtccgg gaggtgtggg cgggcgggga cgttgtttct ccggaggccg
     2401 tgcgccgggt gctcgcacac tgcccggaca tcactgtcca caatggttac ggcccgacgg
     2461 agaccactgt tttcgtcacg gtgcaccgag tggacaaaac gatggctgat gcgcgggcgt
     2521 tgcccatcgg aaccccgttg gcccataccg gtgtctacat cctggatgag gcgctgcgcc
     2581 ttgtcccgcc tggtgtggtg ggtgagcttt atgtggcggg ggcgcatttg gcgcggggtt
     2641 atgtggggcg tgcgggtttg acggcggagc ggtttgtggc t
//
