LOCUS       NZ_BMUU01000040         5664 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Streptomyces xanthochromogenes strain JCM 4594 sequence40, whole
            genome shotgun sequence.
ACCESSION   NZ_BMUU01000040
VERSION     NZ_BMUU01000040.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces xanthochromogenes
  ORGANISM  Streptomyces xanthochromogenes
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 5664)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650355.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:28:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,827
            CDSs (total)                      :: 7,752
            Genes (coding)                    :: 7,642
            CDSs (with protein)               :: 7,642
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 24 of 110
            Pseudo Genes (incomplete)         :: 94 of 110
            Pseudo Genes (internal stop)      :: 7 of 110
            Pseudo Genes (multiple problems)  :: 12 of 110
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:05:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 5664
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUU01000040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..3659)
                     /db_xref="GeneID:96295711"
                     /locus_tag="IE233_RS38680"
     CDS             complement(<1..3659)
                     /NRPS_PKS="Domain: NRPS-COM_Nterm (3-28). E-value: 3.8e-11.
                     Score: 34.0. Matches aSDomain:
                     nrpspksdomains_IE233_RS38680_NRPS-COM_Nterm.1"
                     /NRPS_PKS="Domain: Cglyc (5-304). E-value: 9.1e-122. Score:
                     397.9. Matches aSDomain:
                     nrpspksdomains_IE233_RS38680_Cglyc.1"
                     /NRPS_PKS="Domain: AMP-binding (478-886). E-value:
                     2.6e-107. Score: 350.9. Matches aSDomain:
                     nrpspksdomains_IE233_RS38680_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (991-1059). E-value: 4.4e-30. Score:
                     95.7. Matches aSDomain: nrpspksdomains_IE233_RS38680_PCP.1"
                     /codon_start=1
                     /db_xref="GeneID:96295711"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IE233_RS38680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_190029699.1"
                     /sec_met_domain="Condensation (E-value: 6e-75, bitscore:
                     239.1, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.6e-124, bitscore:
                     402.6, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 2.4e-19, bitscore:
                     56.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSSGLEDILPLSPMQEGLLFHSRYEQDGADVYAVQHVFDIEGTL
                     DAARLRTAVRALVQRHPNLRAGFRQVESGATVQLIPRQVDVPWAEYDLASLPGAEAEAE
                     ILRLAAKEHGIRFDLAQPPLLRFTLVRLAGAHHRLIMTTHHILLDGWSMPILVHELLTL
                     YGSHGDATGLPRPTPYRQYLGWLAQQDRPAAEAAWREVLVGLEQPTLVAAVDPARPALM
                     PERITVELDEDRTTALTDWARRQGVTTNTVLQAAWGLVLSRHTGHDDVVFGAVVAGRDP
                     QLPGVEAMVGLLINMVPVRVVLDPAQSLTETVMRLQDEQTRLTAHHHLGLPRIQQLTGP
                     GDLFDTAMVFENYPWDESAELPDTGLRVTPDLGSGRDATHYPLTLVAAPGRRLYIRLDY
                     RDDLVDRASAAGFLDRLIQVLDLVSTVTATGTDSDPGTGPAATELPVGRIDLLTDEERA
                     TLSAAPEDVARTTGATLPGLFEAWAVGTPDTPAVVCGETALTYRELDERANRLAHVLTT
                     RGIGPEDIVALALPRSTDLVLAVLAVLKAGAAYLPLDPEYPPARLTHMVTDARPALLLT
                     TTETRSSLPTTHDTPVLLLDAPDTTTDLAGRPTHSPTPAFAPDHPAYVIYTSGSTGRPK
                     GVVMPAGVLLNLLEWHHREIGGQPGTRTAQFTAISFDVSAQEVLSALLFGKTLVIPAED
                     VRRDAARFAEWLEDQRVEELFAPNLVLEALAEAAVEQGRRLPRLRTVAQAGEALTPSRL
                     ARAFHRSVPGRVLHNHYGPTETHVVTAHTLGHDLDDWPSSVPIGRPIVNTRAYVLGSGL
                     QLAAPGVVGELYIAGAGVARGYLHRPALTAERFVADPYGSEPGARMYRTGDLVRRNADG
                     DLEFVGRADQQVKIRGFRIEPGEIEAVLTDHPDITQAAVVVREDEPGRALLVAYVTARE
                     ALRPDAVREFTRDRLPDHMVPSAVVLVEHLPLTANGKLDRAALPAPEFASGGSGREART
                     PQEQILCDLFAQVLGLPRVGVDDDFFQLGGHSLLATRLIARIRAAFGVEVGLRTLFEAR
                     TAGAVAARLDTAGPARLALTKQQLPDLVPLSFAQRRLWFLHKMEGPSATYNIPLVLRLT
                     GELDREALRAAVDDVVARHDSLRTVFPEAQDTPYQHVLDSVTVELSTIDISEAELPDAL
                     TSASRHPFDLATEPPLRVDLFRLAPDRHVLLLVLHHIAGDGWSLGPLASDLAGA"
     source          1..5664
                     /culture_collection="JCM:4594"
                     /db_xref="taxon:67384"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces xanthochromogenes"
                     /strain="JCM 4594"
                     /submitter_seqid="sequence40"
                     /type_material="type strain of Streptomyces michiganensis"
     region          1..5664
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..5664
                     /SMILES="N1C(CCC1)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..5664
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:3659](-)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..3659)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS_motif       complement(6..62)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38680_0013"
                     /evalue="3.20E-08"
                     /label="C3_LCL_132-143"
                     /locus_tag="IE233_RS38680"
                     /protein_end="1218"
                     /protein_start="1199"
                     /score="26.1"
                     /tool="antismash"
                     /translation="LHHIAGDGWSLGPLASDLA"
     CDS_motif       complement(258..374)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38680_0012"
                     /evalue="8.90E-21"
                     /label="C2_LCL_024-062"
                     /locus_tag="IE233_RS38680"
                     /protein_end="1134"
                     /protein_start="1095"
                     /score="66.3"
                     /tool="antismash"
                     /translation="ATYNIPLVLRLTGELDREALRAAVDDVVARHDSLRTVFP"
     CDS_motif       complement(393..425)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38680_0011"
                     /evalue="3.20E-03"
                     /label="C1_LCL_004-017"
                     /locus_tag="IE233_RS38680"
                     /protein_end="1089"
                     /protein_start="1078"
                     /score="10.1"
                     /tool="antismash"
                     /translation="SFAQRRLWFLH"
     aSDomain        complement(483..686)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38680_PCP.1"
                     /evalue="4.40E-30"
                     /label="IE233_RS38680_PCP.1"
                     /locus_tag="IE233_RS38680"
                     /protein_end="1059"
                     /protein_start="991"
                     /score="95.7"
                     /tool="antismash"
                     /translation="EQILCDLFAQVLGLPRVGVDDDFFQLGGHSLLATRLIARIRAAFG
                     VEVGLRTLFEARTAGAVAARLDT"
     aSModule        complement(483..3644)
                     /complete
                     /domains="nrpspksdomains_IE233_RS38680_Cglyc.1"
                     /domains="nrpspksdomains_IE233_RS38680_AMP-binding.1"
                     /domains="nrpspksdomains_IE233_RS38680_PCP.1"
                     /locus_tags="IE233_RS38680"
                     /monomer_pairings="Pro -> Pro"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(972..1037)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38680_0010"
                     /evalue="1.40E-12"
                     /label="NRPS-A_a8"
                     /locus_tag="IE233_RS38680"
                     /protein_end="896"
                     /protein_start="874"
                     /score="39.4"
                     /tool="antismash"
                     /translation="FVGRADQQVKIRGFRIEPGEIE"
     aSDomain        complement(1002..2225)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38680_AMP-binding.1"
                     /evalue="2.60E-107"
                     /label="IE233_RS38680_AMP-binding.1"
                     /locus_tag="IE233_RS38680"
                     /protein_end="886"
                     /protein_start="478"
                     /score="350.9"
                     /specificity="substrate consensus: Pro"
                     /tool="antismash"
                     /translation="FEAWAVGTPDTPAVVCGETALTYRELDERANRLAHVLTTRGIGPE
                     DIVALALPRSTDLVLAVLAVLKAGAAYLPLDPEYPPARLTHMVTDARPALLLTTTETRS
                     SLPTTHDTPVLLLDAPDTTTDLAGRPTHSPTPAFAPDHPAYVIYTSGSTGRPKGVVMPA
                     GVLLNLLEWHHREIGGQPGTRTAQFTAISFDVSAQEVLSALLFGKTLVIPAEDVRRDAA
                     RFAEWLEDQRVEELFAPNLVLEALAEAAVEQGRRLPRLRTVAQAGEALTPSRLARAFHR
                     SVPGRVLHNHYGPTETHVVTAHTLGHDLDDWPSSVPIGRPIVNTRAYVLGSGLQLAAPG
                     VVGELYIAGAGVARGYLHRPALTAERFVADPYGSEPGARMYRTGDLVRRNADGDLEFVG
                     RADQQVKIR"
     CDS_motif       complement(1125..1211)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38680_0009"
                     /evalue="3.90E-18"
                     /label="NRPS-A_a6"
                     /locus_tag="IE233_RS38680"
                     /protein_end="845"
                     /protein_start="816"
                     /score="57.5"
                     /tool="antismash"
                     /translation="PGVVGELYIAGAGVARGYLHRPALTAERF"
     CDS_motif       complement(1740..1796)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38680_0008"
                     /evalue="1.10E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="IE233_RS38680"
                     /protein_end="640"
                     /protein_start="621"
                     /score="30.2"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVVMP"
     CDS_motif       complement(1998..2039)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38680_0007"
                     /evalue="6.20E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="IE233_RS38680"
                     /protein_end="554"
                     /protein_start="540"
                     /score="19.3"
                     /tool="antismash"
                     /translation="LAVLKAGAAYLPLD"
     CDS_motif       complement(2598..2711)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38680_0006"
                     /evalue="4.00E-18"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="IE233_RS38680"
                     /protein_end="354"
                     /protein_start="316"
                     /score="57.9"
                     /tool="antismash"
                     /translation="QDEQTRLTAHHHLGLPRIQQLTGPGDLFDTAMVFENYP"
     aSDomain        complement(2748..3644)
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38680_Cglyc.1"
                     /domain_subtypes="Cglyc"
                     /evalue="9.10E-122"
                     /label="IE233_RS38680_Cglyc.1"
                     /locus_tag="IE233_RS38680"
                     /protein_end="304"
                     /protein_start="5"
                     /score="397.9"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHILLDG"
                     /tool="antismash"
                     /translation="LEDILPLSPMQEGLLFHSRYEQDGADVYAVQHVFDIEGTLDAARL
                     RTAVRALVQRHPNLRAGFRQVESGATVQLIPRQVDVPWAEYDLASLPGAEAEAEILRLA
                     AKEHGIRFDLAQPPLLRFTLVRLAGAHHRLIMTTHHILLDGWSMPILVHELLTLYGSHG
                     DATGLPRPTPYRQYLGWLAQQDRPAAEAAWREVLVGLEQPTLVAAVDPARPALMPERIT
                     VELDEDRTTALTDWARRQGVTTNTVLQAAWGLVLSRHTGHDDVVFGAVVAGRDPQLPGV
                     EAMVGLLINMVPVRVVLD"
     CDS_motif       complement(2760..2855)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38680_0005"
                     /evalue="2.50E-18"
                     /label="C5_DCL_263-294"
                     /locus_tag="IE233_RS38680"
                     /protein_end="300"
                     /protein_start="268"
                     /score="58.5"
                     /tool="antismash"
                     /translation="DVVFGAVVAGRDPQLPGVEAMVGLLINMVPVR"
     CDS_motif       complement(3090..3128)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38680_0004"
                     /evalue="4.30E-03"
                     /label="C4_DCL_171-183"
                     /locus_tag="IE233_RS38680"
                     /protein_end="190"
                     /protein_start="177"
                     /score="9.9"
                     /tool="antismash"
                     /translation="PYRQYLGWLAQQD"
     CDS_motif       complement(3168..3230)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38680_0003"
                     /evalue="2.90E-12"
                     /label="C3_DCL_135-156"
                     /locus_tag="IE233_RS38680"
                     /protein_end="164"
                     /protein_start="143"
                     /score="38.9"
                     /tool="antismash"
                     /translation="HHILLDGWSMPILVHELLTLY"
     CDS_motif       complement(3450..3569)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38680_0002"
                     /evalue="1.70E-17"
                     /label="C2_DCL_024-062"
                     /locus_tag="IE233_RS38680"
                     /protein_end="70"
                     /protein_start="30"
                     /score="55.7"
                     /tool="antismash"
                     /translation="DVYAVQHVFDIEGTLDAARLRTAVRALVQRHPNLRAGFRQ"
     aSDomain        complement(3576..3650)
                     /aSDomain="NRPS-COM_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38680_NRPS-COM_Nterm.1"
                     /evalue="3.80E-11"
                     /label="IE233_RS38680_NRPS-COM_Nterm.1"
                     /locus_tag="IE233_RS38680"
                     /protein_end="28"
                     /protein_start="3"
                     /score="34.0"
                     /tool="antismash"
                     /translation="SGLEDILPLSPMQEGLLFHSRYEQD"
     CDS_motif       complement(3591..3629)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38680_0001"
                     /evalue="7.10E-05"
                     /label="C1_DCL_004-017"
                     /locus_tag="IE233_RS38680"
                     /protein_end="23"
                     /protein_start="10"
                     /score="14.8"
                     /tool="antismash"
                     /translation="PLSPMQEGLLFHS"
     CDS             complement(3656..>5660)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (90-156). E-value: 3.1e-23. Score:
                     73.7. Matches aSDomain: nrpspksdomains_IE233_RS38685_PCP.1"
                     /NRPS_PKS="Domain: Epimerization (170-484). E-value:
                     8.5e-138. Score: 452.2. Matches aSDomain:
                     nrpspksdomains_IE233_RS38685_Epimerization.1"
                     /NRPS_PKS="Domain: TIGR01720 (497-660). E-value: 3.9e-37.
                     Score: 119.4. Matches aSDomain:
                     nrpspksdomains_IE233_RS38685_TIGR01720.1"
                     /NRPS_PKS="Domain: NRPS-COM_Cterm (643-664). E-value:
                     5.8e-09. Score: 27.6. Matches aSDomain:
                     nrpspksdomains_IE233_RS38685_NRPS-COM_Cterm.1"
                     /codon_start=3
                     /db_xref="GeneID:96295712"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IE233_RS38685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_229893139.1"
                     /sec_met_domain="PP-binding (E-value: 1.7e-13, bitscore:
                     38.2, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 4.6e-39, bitscore:
                     121.3, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GIAQSAVVVRDDRAGDARLVAYVVTRDDLTPEGLREFARRSLPEY
                     MVPSAFVTLDHLPLTVNGKLDSAALPAPGPAVRADVESREPRTARERLLAELFAQALGM
                     DRVGCDDDFFVLGGDSIVSIRLVSLARSAGIGFTVRDVFEQRTVAGLAQAATELALSTT
                     EFDDSGIGAVEPTPIMRWFDERGGGIDQFHQAMLLEVPADLGAERLTLAVQALVDHHDA
                     LRLTRTPGGTDGRSWSLAVAPVGSVTAAELVHTVDLAGADLDEALVRDHAEAAATRLSA
                     ERGTLLQAVWFDAGPVHPGRLLLLVNHLVVDGVSWRILLPDLIAAWEAVALGRPPRLDP
                     VGTSLRRWSALLAAEARHPARAAESTLWADLLAAADPPLTAGRLDPVRDTVGRASALTL
                     SLPPDVTTPLLTTVPTAFHAGVNDVLLTALGLAVAEWRRGHARGRHTALLVDVEGHGRE
                     EIVEDVDLSRTVGWFTSLHPVRIDPGPLVWEEVVEGGPALGQALKRVKEQLRALPDRGM
                     GFGLLRHLNTATGPELAGLAVPQVGFNYLGRFPSAGTPVVPGHPGWAVASEAGLLSGAD
                     PATALAHGIEVNSMVRDTPDGPWLEAVWTWAPDLWPEPHARALAEQWFLAIRGLVRHAD
                     RSGAGGHTPSDFPLTELSQHDIEQLEAAWRIQK"
     gene            complement(3656..>5664)
                     /db_xref="GeneID:96295712"
                     /locus_tag="IE233_RS38685"
     aSDomain        complement(3668..3730)
                     /aSDomain="NRPS-COM_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38685_NRPS-COM_Cterm.1"
                     /evalue="5.80E-09"
                     /label="IE233_RS38685_NRPS-COM_Cterm.1"
                     /locus_tag="IE233_RS38685"
                     /protein_end="664"
                     /protein_start="643"
                     /score="27.6"
                     /tool="antismash"
                     /translation="PSDFPLTELSQHDIEQLEAAW"
     aSDomain        complement(3680..4168)
                     /aSDomain="TIGR01720"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38685_TIGR01720.1"
                     /evalue="3.90E-37"
                     /label="IE233_RS38685_TIGR01720.1"
                     /locus_tag="IE233_RS38685"
                     /protein_end="660"
                     /protein_start="497"
                     /score="119.4"
                     /tool="antismash"
                     /translation="LGQALKRVKEQLRALPDRGMGFGLLRHLNTATGPELAGLAVPQVG
                     FNYLGRFPSAGTPVVPGHPGWAVASEAGLLSGADPATALAHGIEVNSMVRDTPDGPWLE
                     AVWTWAPDLWPEPHARALAEQWFLAIRGLVRHADRSGAGGHTPSDFPLTELSQHDIEQL
                     "
     aSModule        complement(3680..5389)
                     /domains="nrpspksdomains_IE233_RS38685_PCP.1"
                     /domains="nrpspksdomains_IE233_RS38685_Epimerization.1"
                     /domains="nrpspksdomains_IE233_RS38685_TIGR01720.1"
                     /incomplete
                     /locus_tags="IE233_RS38685"
                     /tool="antismash"
                     /type="unknown"
     CDS_motif       complement(4085..4147)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38685_0005"
                     /evalue="1.10E-10"
                     /label="NRPS-E6"
                     /locus_tag="IE233_RS38685"
                     /protein_end="525"
                     /protein_start="504"
                     /score="33.6"
                     /tool="antismash"
                     /translation="VKEQLRALPDRGMGFGLLRHL"
     aSDomain        complement(4208..5149)
                     /aSDomain="Epimerization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38685_Epimerization.1"
                     /evalue="8.50E-138"
                     /label="IE233_RS38685_Epimerization.1"
                     /locus_tag="IE233_RS38685"
                     /protein_end="484"
                     /protein_start="170"
                     /score="452.2"
                     /specificity="E activity: active"
                     /specificity="Active site motif: NHLVVDG"
                     /tool="antismash"
                     /translation="GAVEPTPIMRWFDERGGGIDQFHQAMLLEVPADLGAERLTLAVQA
                     LVDHHDALRLTRTPGGTDGRSWSLAVAPVGSVTAAELVHTVDLAGADLDEALVRDHAEA
                     AATRLSAERGTLLQAVWFDAGPVHPGRLLLLVNHLVVDGVSWRILLPDLIAAWEAVALG
                     RPPRLDPVGTSLRRWSALLAAEARHPARAAESTLWADLLAAADPPLTAGRLDPVRDTVG
                     RASALTLSLPPDVTTPLLTTVPTAFHAGVNDVLLTALGLAVAEWRRGHARGRHTALLVD
                     VEGHGREEIVEDVDLSRTVGWFTSLHPVRIDPG"
     CDS_motif       complement(4217..4267)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38685_0004"
                     /evalue="2.80E-09"
                     /label="NRPS-E5"
                     /locus_tag="IE233_RS38685"
                     /protein_end="481"
                     /protein_start="464"
                     /score="28.9"
                     /tool="antismash"
                     /translation="DLSRTVGWFTSLHPVRI"
     CDS_motif       complement(4691..4756)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38685_0003"
                     /evalue="9.90E-11"
                     /label="NRPS-E2"
                     /locus_tag="IE233_RS38685"
                     /protein_end="323"
                     /protein_start="301"
                     /score="33.8"
                     /tool="antismash"
                     /translation="LLLLVNHLVVDGVSWRILLPDL"
     CDS_motif       complement(4985..5050)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38685_0002"
                     /evalue="1.10E-04"
                     /label="C2_dual_024-063"
                     /locus_tag="IE233_RS38685"
                     /protein_end="225"
                     /protein_start="203"
                     /score="14.7"
                     /tool="antismash"
                     /translation="LGAERLTLAVQALVDHHDALRL"
     CDS_motif       complement(5108..5137)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38685_0001"
                     /evalue="6.40E-03"
                     /label="NRPS-E1"
                     /locus_tag="IE233_RS38685"
                     /protein_end="184"
                     /protein_start="174"
                     /score="9.8"
                     /tool="antismash"
                     /translation="PTPIMRWFDE"
     aSDomain        complement(5192..5389)
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38685_PCP.1"
                     /evalue="3.10E-23"
                     /label="IE233_RS38685_PCP.1"
                     /locus_tag="IE233_RS38685"
                     /protein_end="156"
                     /protein_start="90"
                     /score="73.7"
                     /tool="antismash"
                     /translation="ERLLAELFAQALGMDRVGCDDDFFVLGGDSIVSIRLVSLARSAGI
                     GFTVRDVFEQRTVAGLAQAAT"
ORIGIN
        1 gcacccgcca aatccgaagc caacggaccc aacgaccacc catcacccgc aatgtgatgc
       61 agcacgagca ggaggacgtg ccggtcgggt gcgaggcgga acaggtcgac gcgcagcggg
      121 ggttcggtcg cgaggtcgaa ggggtggcgg gacgccgacg tgagggcgtc cggcagttcc
      181 gcctcggaga tgtcgatggt ggacagctcg accgtgacgg agtccaggac gtgctgatac
      241 ggagtgtcct gggcttcggg gaacacggtg cgcaggctgt cgtgccgggc caccacgtcg
      301 tccacggccg cgcgcagcgc ctcccggtcc agctcgcccg tcagccgcag gacgagcgga
      361 atgttgtagg tggcgctggg tccctccatc ttgtgcagga accacagtcg acgctgggcg
      421 aaggacagcg gtacgaggtc gggcagttgc tgtttggtca gggccagccg cgcgggcccg
      481 gcggtgtcga ggcgggcggc caccgcgccg gcggtccggg cctcgaagag cgtgcgcagg
      541 ccgacctcga cgccgaaggc ggcccggatg cgggcgatga ggcgagtggc caggagcgag
      601 tgcccgccca gctggaagaa gtcgtcgtcc acacccaccc gcggcagccc gagaacctgc
      661 gcgaacagat cacacaagat ctgctcctgc ggcgtacgcg cctcccgccc cgacccaccc
      721 gacgcgaact ccggcgccgg caacgccgcc cggtccaact tgccgttcgc cgtcagggga
      781 aggtgctcca cgagtacgac ggccgacggc accatgtggt cgggcaaccg gtcccgggtg
      841 aactcccgga cggcgtcggg ccgtagggcc tcccgcgccg tcacgtaggc gaccaggagg
      901 gccctgccgg gctcgtcctc acgcacgacc acggcggcct gggtgatgtc ggggtgatcg
      961 gtcaacacgg cctcgatctc gccgggttcg atacggaacc cccggatctt gacctgctgg
     1021 tcggcgcggc cgacgaattc gaggtcgccg tcggcgttgc ggcgtaccag gtccccggtg
     1081 cgatacatgc gggcaccggg ctcactgccg tacgggtcgg ccacgaaacg ctcggcggtg
     1141 agagcggggc ggtgcagata cccgcgggcc acgcccgcac ccgcgatgta cagctccccc
     1201 accacgcctg gagccgccag ctgcagccca gaacccagca catacgcacg ggtgttgacg
     1261 atcggacggc cgatcggcac ggacgacggc cagtcgtcca ggtcgtgccc caaggtgtgg
     1321 gccgtgacca catgggtctc ggtggggccg tagtggttgt gcagtactcg gcccggcacc
     1381 gaccggtgga acgcacgggc caggcggctc ggtgtcagcg cttcaccggc ctgggcgacg
     1441 gtacgcagcc gcggcagcct tcggccctgc tcgacggcgg cctcggcgag ggcctcaagg
     1501 accaggttcg gcgcgaacaa ctcctccacc cgttggtctt cgagccactc ggcgaagcgg
     1561 gccgcgtcgc gccggacgtc ctcggcgggt atcaccaatg tcttgccgaa gagcagggcc
     1621 gaaaggacct cctgcgccga cacgtcgaag ctgatcgccg tgaactgcgc ggtcctggtg
     1681 cccggttggc cgccgatctc acggtggtgc cactccagga gattgagcag gacgcccgcg
     1741 ggcatgacga cgcccttggg tcgtccggtg gagccggagg tgtagatgac gtacgccggg
     1801 tgatcgggtg cgaaggcggg ggtggggctg tgcgtggggc gcccggcgag gtcggtggtg
     1861 gtgtccgggg cgtcgaggag gaggaccggg gtgtcgtgcg tggtggggag gctggaccgg
     1921 gtctccgtgg tggtgaggag aagggcgggc cgggcgtcgg tgaccatgtg ggtcagccgg
     1981 gcaggggggt attcgggatc gaggggcagg taggcggcgc ctgctttgag gacggcgagg
     2041 acggcgagga cgaggtcggt cgagcggggc agcgcgaggg cgacgatgtc ctccgggccg
     2101 atgccacggg tcgtcagcac atgcgccagc cggttcgccc gctcgtcgag ttcccggtag
     2161 gtgagggccg tctcgccgca gaccaccgcc ggggtgtccg gggtcccgac ggcccacgcc
     2221 tcgaacagcc ccggcagggt ggcccctgtc gtgcgcgcga cgtcttcggg ggcggccgac
     2281 agagtggcgc gctcctcatc ggtgagcagg tcgatccgcc cgacgggcag ctcggttgcg
     2341 gcgggtccgg tgccgggatc ggaatcagta ccggtggcgg tgacggtgga gacgaggtcg
     2401 agcacctgga tcagccggtc gaggaacccg gcggccgagg ccctgtcgac gaggtcgtcc
     2461 cggtagtcga ggcggatgta gaggcgccgc cccggggccg cgaccagggt gagcggatag
     2521 tgggtcgcgt cccgcccgct gccgaggtcc ggggtgacgc gcagcccggt gtccggcagc
     2581 tcggcggatt cgtcccaggg gtagttctcg aacaccatgg ccgtgtcgaa gaggtcgccg
     2641 ggaccggtca gttgctggat gcgcggcagc ccgagatggt ggtgcgcggt cagccgcgtc
     2701 tgctcgtcct ggagccgcat gactgtctcc gtcagcgact gcgccgggtc cagcacgacc
     2761 cggacgggca ccatgttgat gagcagcccc accatggcct cgacaccggg cagttggggg
     2821 tcgcgtcccg ccacgacggc cccgaacacc acgtcgtcgt gccccgtgtg ccggctcagc
     2881 accagacccc aggcggcctg gagaaccgtg ttcgtggtca cgccctgccg acgcgcccag
     2941 tccgtcagtg ccgtcgtgcg gtcctcgtcc agctcgacgg tgatccgctc cggcatgagt
     3001 gcgggccgcg ccggatccac cgcggcgacc agggtcggct gctccaggcc gacgagcacc
     3061 tcgcgccagg cggcctccgc cgccggccgg tcctgctgcg cgagccagcc cagatactgc
     3121 cggtacgggg tcggccgggg cagcccggtg gcgtcgccgt ggctgccgta cagcgtgagc
     3181 agctcgtgga cgaggatggg catcgaccag ccgtcgagca ggatgtgatg ggtggtcatg
     3241 atcagccgat ggtgtgcccc ggccagccgc accagggtga agcgcagcag cggcggctgt
     3301 gccaggtcga agcggatgcc gtgttccttg gcggcgagcc ggaggatttc cgcctccgct
     3361 tcggcgccgg gcaacgaagc gaggtcgtac tcggcccagg gaacgtccac ttgacgaggt
     3421 atcagctgaa cggtcgcgcc cgattcgacc tgccggaagc cggcccgaag gttcgggtgc
     3481 cgctggacga gggcacggac ggccgtccga aggcgcgccg cgtcgagggt gccttcgatg
     3541 tcgaagacgt gctgcaccgc atagacgtcc gcgccgtcct gctcgtagcg ggagtggaac
     3601 aggagcccct cctgcatggg cgagagggga aggatgtctt ccaggcccga cgacgtcatt
     3661 tctgaatcct ccacgcggct tcgagctgct cgatgtcgtg ctggctgagt tcggtcagag
     3721 ggaagtccga gggtgtgtgg ccgcccgcac cggaccggtc ggcgtgccgg accaggccac
     3781 ggatcgccag gaaccactgc tccgcgaggg cgcgggcgtg tggttcgggc cagagatcag
     3841 gagcccaggt ccagacggcc tccaaccacg gcccgtccgg ggtgtcacgg accatggagt
     3901 tgacctcgat gccgtgcgcc agggcggtcg ccggatcggc accgctcaat aggccggcct
     3961 ccgacgccac ggcccatccc ggatggccgg gcaccaccgg agtgcccgcg gacgggaagc
     4021 ggccgaggta gttgaagccg acttggggca cggcgagccc ggccagctcc ggcccggtcg
     4081 cggtgttgag gtggcgcagc agaccgaagc ccatgcctcg gtcgggcagc gcccgcagct
     4141 gttccttgac ccgcttcagc gcctgtccga gagcgggccc gccttccacg acctcctccc
     4201 agaccagtgg tccagggtcg atgcggacgg gatggagcga ggtgaaccag cccaccgtgc
     4261 gcgacaggtc cacgtcctcg acgatctcct cccggccgtg cccctccacg tcgaccagca
     4321 gtgccgtgtg cctcccgcgg gcgtgccccc ggcgccactc ggccaccgcc aggcccagag
     4381 cggtcagcag gacgtcgttc actccggcgt ggaaggccgt gggcaccgtg gtcaggagcg
     4441 gggtggtgac gtcgggcgga agggagagcg tgagcgcact ggcccggccc acggtgtccc
     4501 gaacgggatc gagccggccg gccgtcagcg gtgggtcggc agcggcgagg agatcggccc
     4561 acagggtcga ctccgcggcc cgggccgggt gtcgggcctc cgcggccagc agcgccgacc
     4621 agcggcgcag cgaggtgccc accgggtcga gccggggcgg gcggccgagc gcgaccgcct
     4681 cccaggcggc gatgaggtcc ggaagcagga tgcgccagga cacgccgtcc accaccaggt
     4741 ggttgacgag gagcagcagc ctcccggggt ggaccggtcc ggcatcgaac cagaccgcct
     4801 ggagcagcgt cccccgctcg gccgacagcc gggtggccgc cgcctccgca tggtcccgga
     4861 cgagcgcctc gtcgaggtcg gccccggcga ggtccaccgt gtgcaccagc tcggcggccg
     4921 tcaccgaacc caccggcgcg accgccagtg accacgaccg gccgtccgtg ccacccggcg
     4981 tgcgggtcag gcgcagcgcg tcatggtggt ccacgagtgc ctggacggcc agggtgaggc
     5041 gctccgcgcc cagatcggcc ggcacctcca gcagcatcgc ctgatggaac tggtcgattc
     5101 cgccgccgcg ctcgtcgaac caacgcatga tcggggtcgg ttccaccgcc ccgatgcccg
     5161 agtcgtcgaa ctccgttgtg gacagggcca gttcagtggc cgcttgggcg agtccggcga
     5221 ccgtccgctg ctcgaagacg tcgcggaccg tgaatccgat gcccgccgaa cgtgccaggc
     5281 tgaccagacg gatcgacacg atgctgtccc cgccgagcac gaagaagtcg tcgtcgcacc
     5341 cgacccggtc cattcccaga gcctgcgcga acaactccgc gagcagccgc tcgcgcgcgg
     5401 tgcgcggctc acggctctcc acgtccgccc gcacggccgg gccgggggcg ggcagcgcgg
     5461 cgctgtcgag cttgccgttg accgtcagcg gcaagtggtc cagcgtgacg aacgcggacg
     5521 gcaccatgta ctccggcagg ctgcgccgcg cgaactcgcg aaggccttcc ggcgtcagat
     5581 cgtcgcgggt gacgacgtag gcgacgaggc gggcgtcgcc ggcacggtcg tcccggacca
     5641 ccaccgcgct ctgcgcgatg cccg
//
