LOCUS       NZ_BMUU01000036        10154 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Streptomyces xanthochromogenes strain JCM 4594 sequence36, whole
            genome shotgun sequence.
ACCESSION   NZ_BMUU01000036
VERSION     NZ_BMUU01000036.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces xanthochromogenes
  ORGANISM  Streptomyces xanthochromogenes
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 10154)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650355.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:28:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,827
            CDSs (total)                      :: 7,752
            Genes (coding)                    :: 7,642
            CDSs (with protein)               :: 7,642
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 24 of 110
            Pseudo Genes (incomplete)         :: 94 of 110
            Pseudo Genes (internal stop)      :: 7 of 110
            Pseudo Genes (multiple problems)  :: 12 of 110
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:05:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10154
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUU01000036.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..3945
                     /db_xref="GeneID:96295690"
                     /locus_tag="IE233_RS38570"
     CDS             <1..3945
                     /NRPS_PKS="Domain: Condensation_LCL (1-298). E-value:
                     1.8e-114. Score: 373.7. Matches aSDomain:
                     nrpspksdomains_IE233_RS38570_Condensation_LCL.1"
                     /NRPS_PKS="Domain: AMP-binding (464-864). E-value:
                     1.5e-109. Score: 358.2. Matches aSDomain:
                     nrpspksdomains_IE233_RS38570_AMP-binding.1"
                     /NRPS_PKS="Domain: PCP (970-1036). E-value: 7.1e-28. Score:
                     88.6. Matches aSDomain: nrpspksdomains_IE233_RS38570_PCP.1"
                     /NRPS_PKS="Domain: Thioesterase (1058-1297). E-value:
                     1.6e-35. Score: 115.6. Matches aSDomain:
                     nrpspksdomains_IE233_RS38570_Thioesterase.1"
                     /codon_start=1
                     /db_xref="GeneID:96295690"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thioesterase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010470971.1"
                     /locus_tag="IE233_RS38570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_190029686.1"
                     /sec_met_domain="Condensation (E-value: 5.9e-74, bitscore:
                     237.4, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.4e-120, bitscore:
                     391.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.1e-19, bitscore:
                     59.5, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Thioesterase (E-value: 7.6e-30, bitscore:
                     93.4, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LLPLSAAQRRLWFLAQMEGPAYNSPLALRVSGKVNLPALQAALSD
                     VVERHEALRTVFPEQDGEPFQQVLTAAEAVPELEVVAADEQGLDRALEVAAGRSFDLAK
                     DLLLRARLFTLAEDESVLLLLKHHMVSDGWSTGLLMRDLSTAYTARCQNQAPVWKQLPV
                     HYADYALWQRELLGEEDDPDSVLHQQSAFWRQALAGVPELLELPTDRPRPAVSGHGGAA
                     VPFNVDAELHTRLMALAKARGCTLYMVLQAAFAAFLTRLGAGTDIPIGSAVAGRGDDAL
                     NDLVGFFVNTLVLRTDTSGDPTFDELLDRVRESDLAAYAHQDLPFERLVEILNPPRSLS
                     HHPLFQVMLVLQNNSSSHDGSLHDLELREWPIAQSGAKIDLALTVGETAGGGLAGALEY
                     ATDLFDRATAESMCERLTRLLTVLAHEPGSRIGEADILTPQERRRMLVEWNDTAREVPA
                     ATLPALFEARVAAAPDGVAVVHRDTDLTYAELNARANRLARHLISVGIGPEDVVGLAMP
                     RSPQMIVALLAVVKAGAAYLPIDSEYPPERIAFMLDDAAPVCVITTAATAPGLPTGTKV
                     LALDDPAVGTLLASQPDTDPGDRDRRAPLSLDAPAYVIYTSGSTGRPKGVTVTHHGIAS
                     LVANQAERYDAGPDSRVLQFASLSFDVTVSEYCLSLLSGARLVLPGATIYGEALSDFMR
                     EKEVTHAHIPPAVLSSLPGTHLPDLRVLITGSEALSPELVARWAPGRRMVNAYGPTEAT
                     VDVASWVFEGLTEGERVDDIPIGRPVSNARVFVLDDGLRPVPVGVAGELYVAGAGLARG
                     YLNRPGLTAGRFVANPFGPAGSRLYRTGDLVRWHGHGYLTFLGRVDDQVKVRGFRIELG
                     EIESVLLKDPEVAQSVAVVREDRPGDKRIVAYVVAAAGTAVDAAAVRERAAAMLPEYMV
                     PSVVVVLEALPLTANRKLDRGRLPAPDYSTAHRRAPRTEHERVLCALFGEVLGLEEVSV
                     DDGFFDLGGHSLLATRLLSRIRAELNTEISVRTLFQAPTVAALSRQLQLPSSDDALSPV
                     LPLRSTGRRLPIFCVHPVGGTSWCYSGLLRHFGPDYPLYGIQARGLNGESPLPQSVEEM
                     ARGYVDEILTIQKNGPYRLVGWSLGGTAAHAVAIELQNRGYDVEFLAMLDSSPHPAPLR
                     EESERPEVILRVIMEAFGAEAGGLGESPGMDQAVAAMQKKMSTLGSLAQGQVQRVLEVA
                     LNSARVGTLPRAVGRMSGDLYFYSAVGGRDQGAADAAALWAPYCTGQVHAYEVDCAHGE
                     MMSPEPLSIIAKTLVATVCELEEKRNEESV"
     source          1..10154
                     /culture_collection="JCM:4594"
                     /db_xref="taxon:67384"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces xanthochromogenes"
                     /strain="JCM 4594"
                     /submitter_seqid="sequence36"
                     /type_material="type strain of Streptomyces michiganensis"
     region          1..10154
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..10154
                     /SMILES="NC([*])C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10154
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:>10154](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      <1..>10154
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     aSDomain        7..897
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38570_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="1.80E-114"
                     /label="IE233_RS38570_Condensation_LCL.1"
                     /locus_tag="IE233_RS38570"
                     /protein_end="298"
                     /protein_start="1"
                     /score="373.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHMVSDG"
                     /tool="antismash"
                     /translation="LPLSAAQRRLWFLAQMEGPAYNSPLALRVSGKVNLPALQAALSDV
                     VERHEALRTVFPEQDGEPFQQVLTAAEAVPELEVVAADEQGLDRALEVAAGRSFDLAKD
                     LLLRARLFTLAEDESVLLLLKHHMVSDGWSTGLLMRDLSTAYTARCQNQAPVWKQLPVH
                     YADYALWQRELLGEEDDPDSVLHQQSAFWRQALAGVPELLELPTDRPRPAVSGHGGAAV
                     PFNVDAELHTRLMALAKARGCTLYMVLQAAFAAFLTRLGAGTDIPIGSAVAGRGDDALN
                     DLVGFFVNTLVLRTDT"
     aSModule        7..3894
                     /complete
                     /domains="nrpspksdomains_IE233_RS38570_Condensation_LCL.1"
                     /domains="nrpspksdomains_IE233_RS38570_AMP-binding.1"
                     /domains="nrpspksdomains_IE233_RS38570_PCP.1"
                     /domains="nrpspksdomains_IE233_RS38570_Thioesterase.1"
                     /final_module
                     /locus_tags="IE233_RS38570"
                     /monomer_pairings="X -> X"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       61..177
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38570_0001"
                     /evalue="4.30E-18"
                     /label="C2_LCL_024-062"
                     /locus_tag="IE233_RS38570"
                     /protein_end="58"
                     /protein_start="19"
                     /score="57.7"
                     /tool="antismash"
                     /translation="PAYNSPLALRVSGKVNLPALQAALSDVVERHEALRTVFP"
     CDS_motif       382..444
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38570_0002"
                     /evalue="2.10E-10"
                     /label="C3_LCL_132-143"
                     /locus_tag="IE233_RS38570"
                     /protein_end="147"
                     /protein_start="126"
                     /score="33.0"
                     /tool="antismash"
                     /translation="HHMVSDGWSTGLLMRDLSTAY"
     CDS_motif       493..531
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38570_0003"
                     /evalue="9.30E-06"
                     /label="C4_LCL_164-176"
                     /locus_tag="IE233_RS38570"
                     /protein_end="176"
                     /protein_start="163"
                     /score="18.1"
                     /tool="antismash"
                     /translation="HYADYALWQRELL"
     CDS_motif       730..783
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38570_0004"
                     /evalue="5.10E-01"
                     /label="Cy4"
                     /locus_tag="IE233_RS38570"
                     /protein_end="260"
                     /protein_start="242"
                     /score="3.7"
                     /tool="antismash"
                     /translation="GCTLYMVLQAAFAAFLTR"
     CDS_motif       799..888
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38570_0005"
                     /evalue="6.20E-13"
                     /label="C5_LCL_267-296"
                     /locus_tag="IE233_RS38570"
                     /protein_end="295"
                     /protein_start="265"
                     /score="41.1"
                     /tool="antismash"
                     /translation="DIPIGSAVAGRGDDALNDLVGFFVNTLVLR"
     CDS_motif       937..1056
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38570_0006"
                     /evalue="1.40E-23"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="IE233_RS38570"
                     /protein_end="351"
                     /protein_start="311"
                     /score="75.4"
                     /tool="antismash"
                     /translation="RESDLAAYAHQDLPFERLVEILNPPRSLSHHPLFQVMLVL"
     aSDomain        1396..2595
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38570_AMP-binding.1"
                     /evalue="1.50E-109"
                     /label="IE233_RS38570_AMP-binding.1"
                     /locus_tag="IE233_RS38570"
                     /protein_end="864"
                     /protein_start="464"
                     /score="358.2"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FEARVAAAPDGVAVVHRDTDLTYAELNARANRLARHLISVGIGPE
                     DVVGLAMPRSPQMIVALLAVVKAGAAYLPIDSEYPPERIAFMLDDAAPVCVITTAATAP
                     GLPTGTKVLALDDPAVGTLLASQPDTDPGDRDRRAPLSLDAPAYVIYTSGSTGRPKGVT
                     VTHHGIASLVANQAERYDAGPDSRVLQFASLSFDVTVSEYCLSLLSGARLVLPGATIYG
                     EALSDFMREKEVTHAHIPPAVLSSLPGTHLPDLRVLITGSEALSPELVARWAPGRRMVN
                     AYGPTEATVDVASWVFEGLTEGERVDDIPIGRPVSNARVFVLDDGLRPVPVGVAGELYV
                     AGAGLARGYLNRPGLTAGRFVANPFGPAGSRLYRTGDLVRWHGHGYLTFLGRVDDQVKV
                     R"
     CDS_motif       1582..1623
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38570_0007"
                     /evalue="4.30E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="IE233_RS38570"
                     /protein_end="540"
                     /protein_start="526"
                     /score="16.7"
                     /tool="antismash"
                     /translation="LAVVKAGAAYLPID"
     CDS_motif       1834..1893
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38570_0008"
                     /evalue="2.00E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="IE233_RS38570"
                     /protein_end="630"
                     /protein_start="610"
                     /score="32.5"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVTVTH"
     CDS_motif       2386..2475
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38570_0009"
                     /evalue="3.50E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="IE233_RS38570"
                     /protein_end="824"
                     /protein_start="794"
                     /score="54.5"
                     /tool="antismash"
                     /translation="PVGVAGELYVAGAGLARGYLNRPGLTAGRF"
     CDS_motif       2560..2625
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38570_0010"
                     /evalue="9.90E-13"
                     /label="NRPS-A_a8"
                     /locus_tag="IE233_RS38570"
                     /protein_end="874"
                     /protein_start="852"
                     /score="39.9"
                     /tool="antismash"
                     /translation="FLGRVDDQVKVRGFRIELGEIE"
     aSDomain        2914..3111
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38570_PCP.1"
                     /evalue="7.10E-28"
                     /label="IE233_RS38570_PCP.1"
                     /locus_tag="IE233_RS38570"
                     /protein_end="1036"
                     /protein_start="970"
                     /score="88.6"
                     /tool="antismash"
                     /translation="ERVLCALFGEVLGLEEVSVDDGFFDLGGHSLLATRLLSRIRAELN
                     TEISVRTLFQAPTVAALSRQL"
     CDS_motif       2920..2952
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38570_0011"
                     /evalue="4.90E-02"
                     /label="Cy4"
                     /locus_tag="IE233_RS38570"
                     /protein_end="983"
                     /protein_start="972"
                     /score="6.9"
                     /tool="antismash"
                     /translation="VLCALFGEVLG"
     CDS_motif       3178..3207
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38570_0012"
                     /evalue="6.30E-03"
                     /label="NRPS-beforete1"
                     /locus_tag="IE233_RS38570"
                     /protein_end="1068"
                     /protein_start="1058"
                     /score="9.7"
                     /tool="antismash"
                     /translation="PIFCVHPVGG"
     aSDomain        3178..3894
                     /ASF="active site serine present: True"
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38570_Thioesterase.1"
                     /evalue="1.60E-35"
                     /label="IE233_RS38570_Thioesterase.1"
                     /locus_tag="IE233_RS38570"
                     /protein_end="1297"
                     /protein_start="1058"
                     /score="115.6"
                     /tool="antismash"
                     /translation="PIFCVHPVGGTSWCYSGLLRHFGPDYPLYGIQARGLNGESPLPQS
                     VEEMARGYVDEILTIQKNGPYRLVGWSLGGTAAHAVAIELQNRGYDVEFLAMLDSSPHP
                     APLREESERPEVILRVIMEAFGAEAGGLGESPGMDQAVAAMQKKMSTLGSLAQGQVQRV
                     LEVALNSARVGTLPRAVGRMSGDLYFYSAVGGRDQGAADAAALWAPYCTGQVHAYEVDC
                     AHGEMMSPEPLSIIAKT"
     CDS_motif       3358..3432
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38570_0013"
                     /evalue="1.70E-11"
                     /label="NRPS-te1"
                     /locus_tag="IE233_RS38570"
                     /protein_end="1143"
                     /protein_start="1118"
                     /score="36.4"
                     /tool="antismash"
                     /translation="QKNGPYRLVGWSLGGTAAHAVAIEL"
     gene            3929..4144
                     /db_xref="GeneID:96295691"
                     /locus_tag="IE233_RS38575"
                     /old_locus_tag="GCM10010326_78730"
     CDS             3929..4144
                     /codon_start=1
                     /db_xref="GeneID:96295691"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1009: mbtH-like protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009717861.1"
                     /locus_tag="IE233_RS38575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_78730"
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="WP_161258249.1"
                     /transl_table=11
                     /translation="MKNPFERDDVDYHALVNEEGQYSLWPTTIEVPAGWRVTYGPSDRQ
                     SVVNFIEGAWTDMRPKSLVDAMNNPT"
     gene            4196..5416
                     /db_xref="GeneID:96295692"
                     /gene="txtE"
                     /locus_tag="IE233_RS38580"
                     /old_locus_tag="GCM10010326_78740"
     CDS             4196..5416
                     /codon_start=1
                     /db_xref="GeneID:96295692"
                     /gene="txtE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006379043.1"
                     /locus_tag="IE233_RS38580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_78740"
                     /product="4-nitrotryptophan synthase"
                     /protein_id="WP_161258234.1"
                     /sec_met_domain="p450 (E-value: 1.7e-26, bitscore: 81.6,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGNLSPLSNPEIIPNPYPVYADLAEREPVHWCDGLNAWAVLRHED
                     CTGVLQDPRFKAERMEEILSGKFDGRTLPADNIYNRFTSNVMMYTDAPRHGALRKSTHG
                     GFTRAAHEHYNEVIKRVAHDLVAGIPDDANVIDAVVELAEKLPVNAAVHAFGVPEEDLD
                     TVIPSVDKVMTYWSGPQEQPVELDQLLEHLTDLHTYALELVQGKRGTVVEDTVIARLAA
                     GLADQTDVTPEQTVHQLVLLLIALFAPTTPGSLGSGTLAFATNPDQIARFLENPACVDN
                     VANEVVRYNASNQFTWRVAATDVELRGVRIKEGQRVALFLGAANRDPDVFENPDKFDLD
                     RKNSGKNLSFGFGPHACLGRQIASLEIKWFFVSLFERFPVIRPAGEPEWNSNLEFRSLR
                     SLPLSLG"
     gene            5436..6614
                     /db_xref="GeneID:96295693"
                     /locus_tag="IE233_RS38585"
                     /old_locus_tag="GCM10010326_78750"
     CDS             5436..6614
                     /codon_start=1
                     /db_xref="GeneID:96295693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743465.1"
                     /locus_tag="IE233_RS38585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_78750"
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="WP_161258231.1"
                     /transl_table=11
                     /translation="MDTEPLLASTPHRHASSTASGSIADLGEAEEFLRQFYAEQPEQPV
                     PFAVRLGQVVAEVDTTGTYRHTSEELAFGARVAWRNSSRCIGRAYWNSLRVLDRRDVTT
                     PEDIHRDLCEHLRRATNAGRIRPVISIFAPDTPERPAPRVWNDQLIRYGGYRRQDGTVL
                     GDAAYVDFTNVVREMGWLAPQGPFDVLPLVIETADDKPQLFEVPSDLIREVAITHPDQP
                     RISNLGLRWYAVPAISNMRLRIGGIDYPLAPFNGWYMGTEIGARNLVDEDRFNLLPTVA
                     ACLGLDTSRETTLWRDRALVELNVAVLHSFTAAGITISDHHTESRLFLNHLAREERNGR
                     TVSVDWSWIVPPISGGVTPVFHRYYDEVEQRPNFYLDDDARARSRGQCPYAH"
     gene            6641..7648
                     /db_xref="GeneID:96295694"
                     /locus_tag="IE233_RS38590"
                     /old_locus_tag="GCM10010326_78760"
     CDS             6641..7648
                     /EC_number="2.1.-.-"
                     /GO_function="GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96295694"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1042: O-methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539962.1"
                     /locus_tag="IE233_RS38590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_78760"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_161258229.1"
                     /transl_table=11
                     /translation="MIDAQEARSWKSAAQLYNSAVAASAIGAAWELGALDELQDEGTLD
                     AHEFAARNDLDPAATLAMFRALAAVNIVERDGTRVTPAADFAEIQQTRSFFHWLTRGSG
                     ELFRQMPLVLQNQHRKGEIDPRDPAAVTYACREINTLTYDPWFWSAVDGIDFDFTMVAD
                     LGCGNGDRLIKILGRRPGANGIGIDIAADSLKVAESDAAGAGLSDRLTFVTSDVRTMDR
                     RPEFTEVELLTCFMMGHDFWPRERCVATLARIRELFPNVKRFLLGDATRTVGTADQELP
                     VFTLGFEVAHDMMGVFLPTVADWESAFAESGWRVRHKHAIDMTVGEVIFELEPA"
     gene            7752..>10154
                     /db_xref="GeneID:96295695"
                     /locus_tag="IE233_RS38595"
     CDS             7752..>10154
                     /NRPS_PKS="Domain: Cglyc (4-262). E-value: 3.7e-25. Score:
                     80.7. Matches aSDomain:
                     nrpspksdomains_IE233_RS38595_Cglyc.1"
                     /NRPS_PKS="Domain: AMP-binding (442-800). E-value: 2e-93.
                     Score: 305.1. Matches aSDomain:
                     nrpspksdomains_IE233_RS38595_AMP-binding.1"
                     /codon_start=1
                     /db_xref="GeneID:96295695"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012711.5"
                     /locus_tag="IE233_RS38595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_190029687.1"
                     /sec_met_domain="Condensation (E-value: 2.5e-13, bitscore:
                     38.4, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 3.7e-87, bitscore:
                     281.1, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVAQEGLLFHAEVERDLRGLDTTQVVLTVDGHLDVEALHIRAAR
                     VAGAHPGLLARFDRDALGGPVCVARPGARLPLVQRHVPAGVEADATAQRMAEDEWEHAF
                     DPTGDEPLVRLVVITTGQGSRHHLVLTGHRAVLTSEAARDILAAVLAGDPRDGTHGASK
                     DTAEAGRPSEAGRPSEADTRIAWREALRDLDEPCLLGHAAATVPDGEPRRRSESLSVHV
                     PPAIGSLPGRLGVAPEAFYQTVWALVCMKLKNRREAVLGDADDLPVRIVAAPHDTVADV
                     VRRTDASNGLLAVHRHASAATLAEASGLAGAVFDTAVVVDTRRWDRASLGLGEPGEDEP
                     AGPAVTGVRLRDTTHHAATLVVRTDGDGGPPGVELSHRADRLAADTARTAADLFARALA
                     VLATEPGVRVGAIDWLGDSLRHDLLVDYNDTTARLPQAPLHELFTEQRDRTPDAVAVVC
                     EGRALTYAGLDTAANRLAHLIIGHGVRPGDNVALYLSRSAELIIAELAVLKAGAAYVPL
                     DPRQPAQRLSWIVTDAGATLLLTDRQPADLPFTCDIPWARVPAGEDADAVGDPGAPDVD
                     VHPDQLAYVMYTSGSTGTPKGVANTHRNVVELALDPWWDAARHHRVLAYSPPAFDSSTY
                     EMWVPLLHGGGLTVLTGDKIDLGDLAGAVAEHGITALYLTTALFDAMAQEHVEALSALD
                     EIWTGGDVLSTTALRRVLDACPQTTVVHVYGPTETTVFCSYQTFAPGERPVESLHLGRP
                     MANTALYVLDESLAPAAPGVVGELYVAGSHLARGYVGRAGLTAERFVA"
     aSDomain        7764..8537
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38595_Cglyc.1"
                     /domain_subtypes="Cglyc"
                     /evalue="3.70E-25"
                     /label="IE233_RS38595_Cglyc.1"
                     /locus_tag="IE233_RS38595"
                     /protein_end="262"
                     /protein_start="4"
                     /score="80.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHLVLTG"
                     /tool="antismash"
                     /translation="QEGLLFHAEVERDLRGLDTTQVVLTVDGHLDVEALHIRAARVAGA
                     HPGLLARFDRDALGGPVCVARPGARLPLVQRHVPAGVEADATAQRMAEDEWEHAFDPTG
                     DEPLVRLVVITTGQGSRHHLVLTGHRAVLTSEAARDILAAVLAGDPRDGTHGASKDTAE
                     AGRPSEAGRPSEADTRIAWREALRDLDEPCLLGHAAATVPDGEPRRRSESLSVHVPPAI
                     GSLPGRLGVAPEAFYQTVWALVCMKLKNRREAVLGD"
     aSModule        7764..10151
                     /domains="nrpspksdomains_IE233_RS38595_Cglyc.1"
                     /domains="nrpspksdomains_IE233_RS38595_AMP-binding.1"
                     /incomplete
                     /locus_tags="IE233_RS38595"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       8841..8882
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38595_0001"
                     /evalue="3.10E+01"
                     /label="NRPS-A_a3"
                     /locus_tag="IE233_RS38595"
                     /protein_end="377"
                     /protein_start="363"
                     /score="-1.4"
                     /tool="antismash"
                     /translation="TDGDGGPPGVELSH"
     CDS_motif       9075..9119
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38595_0002"
                     /evalue="9.90E+01"
                     /label="NRPS-A_a3"
                     /locus_tag="IE233_RS38595"
                     /protein_end="456"
                     /protein_start="441"
                     /score="-3.3"
                     /tool="antismash"
                     /translation="LFTEQRDRTPDAVAV"
     aSDomain        9078..10151
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS38595_AMP-binding.1"
                     /evalue="2.00E-93"
                     /label="IE233_RS38595_AMP-binding.1"
                     /locus_tag="IE233_RS38595"
                     /protein_end="800"
                     /protein_start="442"
                     /score="305.1"
                     /specificity="substrate consensus: Val"
                     /tool="antismash"
                     /translation="FTEQRDRTPDAVAVVCEGRALTYAGLDTAANRLAHLIIGHGVRPG
                     DNVALYLSRSAELIIAELAVLKAGAAYVPLDPRQPAQRLSWIVTDAGATLLLTDRQPAD
                     LPFTCDIPWARVPAGEDADAVGDPGAPDVDVHPDQLAYVMYTSGSTGTPKGVANTHRNV
                     VELALDPWWDAARHHRVLAYSPPAFDSSTYEMWVPLLHGGGLTVLTGDKIDLGDLAGAV
                     AEHGITALYLTTALFDAMAQEHVEALSALDEIWTGGDVLSTTALRRVLDACPQTTVVHV
                     YGPTETTVFCSYQTFAPGERPVESLHLGRPMANTALYVLDESLAPAAPGVVGELYVAGS
                     HLARGYVGRAGLTAERFV"
     CDS_motif       9264..9305
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38595_0003"
                     /evalue="1.20E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="IE233_RS38595"
                     /protein_end="518"
                     /protein_start="504"
                     /score="21.4"
                     /tool="antismash"
                     /translation="LAVLKAGAAYVPLD"
     CDS_motif       9498..9557
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38595_0004"
                     /evalue="4.10E-10"
                     /label="NRPS-A_a3"
                     /locus_tag="IE233_RS38595"
                     /protein_end="602"
                     /protein_start="582"
                     /score="31.5"
                     /tool="antismash"
                     /translation="AYVMYTSGSTGTPKGVANTH"
     CDS_motif       10062..10148
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS38595_0005"
                     /evalue="1.20E-14"
                     /label="NRPS-A_a6"
                     /locus_tag="IE233_RS38595"
                     /protein_end="799"
                     /protein_start="770"
                     /score="46.4"
                     /tool="antismash"
                     /translation="PGVVGELYVAGSHLARGYVGRAGLTAERF"
ORIGIN
        1 ctgttgccgt tgtcggccgc gcagcgcagg ctgtggttcc tcgcccagat ggagggaccc
       61 gcctacaact ctcccctggc gctgcgtgtg tcggggaagg tgaacctacc cgcgctgcag
      121 gcggcgctgt ccgatgtggt ggagcggcac gaggccctgc gcaccgtgtt ccccgagcag
      181 gacggcgaac ccttccagca ggtgctcacc gctgctgagg cggtgccgga gctggaggtc
      241 gtcgctgccg acgaacaggg cctggaccgg gccctggagg tggcggccgg ccggtctttc
      301 gacctggcca aggatctgct gctgcgggca cggctgttca ccctggccga ggacgagtcc
      361 gtgttgctgc tcctgaagca ccacatggtc agtgacggct ggtcgacggg gctcctgatg
      421 cgggacctgt cgaccgccta caccgcccgc tgtcagaacc aggcgcccgt gtggaagcaa
      481 ctcccggtcc actacgccga ttacgcgctg tggcagcgtg agctgctcgg cgaggaggac
      541 gacccggaca gcgtgctgca tcagcagtcc gccttctggc gtcaagccct ggccggggta
      601 cccgaactgc tggaactgcc caccgaccgg ccccgcccgg ccgtatccgg acatggcggc
      661 gccgccgtcc ccttcaacgt cgacgccgaa ctgcacaccc ggctgatggc gctggccaag
      721 gcccgcggct gcaccctgta catggtcctg caggcagcgt tcgccgcgtt cctcacccgt
      781 ctcggcgcgg gcacggacat tccgatcgga tcagcggtgg ccgggcgcgg ggacgacgcc
      841 ctcaacgacc tggtcggctt cttcgtcaac acgctcgtac tgcgcacgga cacctccggg
      901 gatcccacct tcgacgagct cttggaccgg gtccgcgaga gcgacctggc cgcctacgcc
      961 caccaggacc tcccgttcga acgactcgtc gaaatcctca acccgccccg cagcctgtcc
     1021 caccaccccc tcttccaagt catgctcgtg ctccagaaca acagcagcag ccacgacggc
     1081 tcgctgcatg atctggagct ccgcgagtgg ccgatcgccc agtccggagc caagatcgat
     1141 ctcgctctga ccgtggggga gacagctggt ggcggactgg caggggcact ggagtacgcc
     1201 accgacctgt tcgatcgggc gaccgcggag agcatgtgcg aacggctgac ccgtctgctc
     1261 accgtccttg ctcacgaacc cgggagccgc atcggcgagg cggacatcct caccccgcag
     1321 gagcggcggc ggatgctggt ggagtggaac gacaccgccc gtgaagtccc cgcggccacg
     1381 ttgccggcgc ttttcgaggc cagggtggcg gcagcaccgg acggcgtggc ggtcgtccac
     1441 cgggacaccg acctgaccta tgccgagctg aacgccaggg ccaaccgcct tgcccgccac
     1501 ctgatcagcg ttggcatcgg ccccgaggac gtggtcgggc tggcgatgcc gaggtcgccg
     1561 cagatgatcg tggccctgct ggccgtggtc aaggcaggtg ctgcgtacct gccgatcgac
     1621 agcgagtatc cgcccgagcg gatcgcgttc atgctcgatg acgccgcccc tgtgtgcgtg
     1681 atcaccacgg ccgctacggc acccggcctg cccaccggaa ccaaggtgct ggcactggac
     1741 gatcccgcgg tcgggaccct cctcgcgtcc cagccggaca ccgaccccgg tgaccgggac
     1801 cggcgcgcgc cgctgtccct ggacgctccc gcctacgtca tctacacctc cggctccacc
     1861 ggacgcccca agggcgtcac cgtcacccac cacgggatcg cgagcctcgt cgccaaccag
     1921 gccgaacgct atgacgccgg cccggacagc agagtcctcc agttcgcctc cctcagcttc
     1981 gacgtgaccg tttccgaata ctgcctctcc ctgctgtccg gggcacggct ggtccttccg
     2041 ggggcgacga tctacggaga ggccctcagc gacttcatgc gtgagaagga agtcacgcac
     2101 gcccacatcc ctcccgcggt gttgagcagc cttcccggca cgcacctgcc ggacctgagg
     2161 gtgttgatca cgggcagcga ggcgttgtca cccgaactgg tggcgcgctg ggctccgggc
     2221 cggaggatgg tcaacgcata tggccctacg gaggcgaccg ttgacgtggc gtcctgggtc
     2281 ttcgaggggc tcaccgaggg cgagcgggtg gacgacattc ccatcggccg tcccgtctcc
     2341 aacgcgcggg tgttcgtgct ggacgacggt ttgaggccgg ttccggtggg cgtcgcgggc
     2401 gagttgtacg tcgcgggcgc gggtcttgct cgtggctatc tgaaccgacc ggggctgacc
     2461 gcgggccggt tcgtggcgaa cccgttcggt ccggcgggat cgcggctgta ccgcaccggc
     2521 gatctggtgc ggtggcacgg gcacgggtat ctgacgttcc tggggcgggt ggacgaccag
     2581 gtcaaggtgc gcgggttccg catcgagctg ggtgagatcg agtcggtgct gctgaaggac
     2641 ccggaggtcg cacagtcggt cgccgtggta cgggaggacc gtcccgggga caagcggatc
     2701 gtcgcgtatg tggtcgctgc cgccggtacg gcggtggatg cggccgccgt gcgtgagcgt
     2761 gccgcggcga tgctgccgga gtacatggtg ccttcggtgg tggtggtgct tgaggccctg
     2821 ccgctgaccg cgaacaggaa gctcgaccgc gggcggctgc ccgctccgga ttactccacc
     2881 gcgcaccggc gggctccgcg cacggaacac gagagggtgc tgtgtgcact gttcggcgag
     2941 gtcctcggcc ttgaagaggt gtccgtcgac gacgggttct tcgatctggg cgggcattcg
     3001 ctgctcgcga cccgcctgct cagccgcatc cgagcggagc tgaacaccga gatctccgtg
     3061 cgcacgctct tccaggctcc caccgtggcc gccctgagcc ggcagctgca gctcccctcc
     3121 tcggacgacg cgctcagccc ggtccttccg ctccgctcca ccggaagacg cctccccatc
     3181 ttctgtgtgc acccggtcgg tggtacgagc tggtgctact ccggattgct tcggcacttc
     3241 ggccccgact atccgttgta cggaatccag gcccgcgggc tgaacgggga atctcccctg
     3301 ccgcagtccg tcgaggaaat ggctcggggc tatgtcgacg aaatcctcac cattcaaaag
     3361 aatggtccgt atcgcctggt cggatggtcg ctcggtggaa ccgcggcgca tgcggtggcc
     3421 atagaattgc aaaatcgtgg ttatgacgtc gagttccttg ctatgctcga ctcgtctccg
     3481 caccccgctc cgctgcggga ggaatccgaa cgtcctgaag tcatcctcag ggtcattatg
     3541 gaggcatttg gggcggaggc cggcggcctg ggtgaatcac ccggcatgga ccaggccgtt
     3601 gcggccatgc agaaaaagat gagcacgctg ggatctttgg cgcagggtca ggtgcagaga
     3661 gttctcgagg tggccctgaa ctcggcccga gtcggcactc ttccccgtgc ggtgggccgg
     3721 atgtccggtg acctgtactt ctattccgca gtcggcggca gggatcaggg tgcggcagac
     3781 gccgcggctc tgtgggcgcc gtattgcacc ggtcaggtcc acgcatacga agtcgactgt
     3841 gcacacgggg aaatgatgtc tcctgagccc ctgtcgatca ttgccaagac tttggtggcc
     3901 acggtgtgtg agctggagga aaaaaggaat gaagaatccg tttgagcggg acgatgtcga
     3961 ctaccacgct ctcgtgaatg aggagggcca gtattcgctg tggcccacca cgatcgaggt
     4021 tcccgcggga tggagggtca cgtacggccc ctcggacagg cagtcggtcg tgaactttat
     4081 cgaaggcgca tggacggaca tgcggcccaa gagcctggtc gacgccatga acaacccgac
     4141 ctagcggtcg aaatccgaag tgaatagttt tttcgaatct cgcagggagc attccgtggg
     4201 gaatctgtca ccgctgtcga atcctgaaat catcccgaac ccctaccccg tctacgcgga
     4261 cctcgcggag cgggagccgg tccactggtg cgacggtttg aacgcgtggg ccgtgttgcg
     4321 gcacgaggac tgcacgggcg tgctgcagga tccccggttc aaggccgaac gcatggagga
     4381 gatcctcagc ggcaagttcg acggccggac cctgccggcc gacaacattt acaaccgatt
     4441 caccagcaac gtcatgatgt acacggacgc tccgcgtcac ggcgccctgc ggaaatccac
     4501 gcacggtggc ttcacgaggg cggcgcacga gcactacaac gaggtcatca agcgcgtcgc
     4561 ccacgatctg gtggccggca tcccggacga cgcgaacgtc atcgacgcgg tcgtcgagct
     4621 ggccgaaaag cttcctgtga acgccgcggt gcacgccttc ggagttcccg aagaggacct
     4681 cgacaccgtg atacccagcg tggacaaggt catgacctac tggtcggggc cccaggagca
     4741 gccggtggag ctcgaccagc tgctggagca cctcacggat ctgcacacct acgcgctcga
     4801 actcgtccag gggaagcggg gcacggtcgt cgaggacacg gtgatcgccc gtcttgcggc
     4861 cggcctcgcg gaccagaccg acgtcacgcc ggagcagacg gtgcatcagc tggtcctgtt
     4921 gctgatcgct ctgttcgcac ccacgacgcc aggttccctc ggcagcggca cgctcgcctt
     4981 cgcgacgaac cccgaccaga tcgcacgctt cctggagaac cccgcgtgcg tcgacaacgt
     5041 ggcgaacgaa gtagtccgct acaacgcgtc gaaccagttc acgtggcgtg ttgcggcgac
     5101 ggacgtggaa ctgcgcggtg tccgcatcaa ggaggggcag cgggttgccc tcttcctcgg
     5161 ggcggcgaac cgggacccgg acgttttcga gaacccggac aagttcgacc tcgaccggaa
     5221 gaacagcggc aagaacctgt cgttcggctt cgggccgcac gcatgtctcg gccgacagat
     5281 cgccagcctc gaaatcaagt ggttcttcgt ttccctgttc gagcgtttcc cggtgatccg
     5341 gcccgcgggc gagcccgagt ggaacagcaa cctcgagttc cggtcgctgc ggtcccttcc
     5401 gctgagcctg ggctgacgga gctccaagaa atcccatgga taccgaaccg ctcctggcaa
     5461 gcactcctca ccggcacgcc tcgtccaccg cgagcggcag catcgccgac ctcggcgaag
     5521 cggaggaatt cctccgccag ttctacgccg aacagcccga gcaacctgtc ccgttcgccg
     5581 tgcgactggg gcaggtggtc gccgaggtcg acaccacggg cacctaccgg cacacctccg
     5641 aggagctggc cttcggtgcc cgggtggcct ggcgcaactc cagccgttgt attggccggg
     5701 cctactggaa cagccttcgc gtgctggacc ggcgggacgt gacgacgccc gaggacatcc
     5761 accgcgacct ctgcgaacac ctgcgccgag cgacgaatgc aggacgcatc cgtccggtca
     5821 tctcgatctt cgcccccgac accccggagc ggcccgcccc cagggtgtgg aacgaccagc
     5881 tcatcaggta cggcggctac cgtcggcagg acgggaccgt tctgggcgat gcggcgtacg
     5941 tcgacttcac caacgtcgtc cgcgagatgg gctggctggc gcctcaaggc cccttcgacg
     6001 tgttgccgct ggtcatcgag accgccgacg acaagccgca gctcttcgag gtgccgtccg
     6061 atctgatccg tgaagtggcc atcacccacc ccgaccagcc gcggatctcg aatctgggtc
     6121 tgcgctggta cgcggtaccg gcgatctcca acatgcgcct gcgcatcgga ggaatcgact
     6181 atcccctggc gcccttcaac ggctggtaca tgggtaccga gatcggcgcg cgcaacctgg
     6241 tcgacgagga ccggttcaac ctgttgccga ccgttgccgc atgcctcggc ctggacacca
     6301 gcagggaaac caccctgtgg cgtgaccgcg cgctggtcga gctcaacgtc gccgtcctgc
     6361 attccttcac cgctgccggc atcacgatca gcgaccacca caccgaatcg cgcctcttcc
     6421 tgaaccactt ggcccgggaa gaacgcaacg gccgcacggt gtccgtggat tggagctgga
     6481 tcgttccccc gatctccggt ggcgtcacgc ctgtctttca tcgctactac gacgaagtcg
     6541 aacagcgccc caatttctac ctcgacgacg atgcccgggc tcgcagccgg gggcaatgcc
     6601 cgtacgcgca ctgaccacca cagcaatcag gaggacagca atgatcgacg cccaggaggc
     6661 ccgctcgtgg aagtcggcgg cacagctcta caactcggcc gtcgccgcat cggccatcgg
     6721 agccgcttgg gagttgggcg cgctggacga gctgcaggac gaagggacgc ttgacgccca
     6781 cgagttcgcg gccaggaacg acctggaccc ggcggcgacc ctcgcgatgt tcagggcgct
     6841 ggccgcggtg aacatcgtgg aaagggacgg cacgcgcgtg acccccgcgg cggacttcgc
     6901 cgagatccag cagacccggt cgttcttcca ctggctcacc aggggaagcg gagagctgtt
     6961 ccgccagatg ccgctggttc tccagaacca gcaccgcaag ggcgagatcg atccgaggga
     7021 tccggcggcg gtcacctacg cgtgccgcga gatcaacacc ctcacctacg acccctggtt
     7081 ctggtcggcc gtcgacggga tcgacttcga cttcaccatg gtcgccgacc tcggatgcgg
     7141 caacggcgat cggctcatca agatcctggg acgccgaccg ggagccaacg ggatcggcat
     7201 agacatcgcc gccgactcgc tgaaggtggc cgagtcggac gctgccggtg ccgggctttc
     7261 cgaccgtctg accttcgtca cctccgacgt gcgaaccatg gatcggcgtc ccgagttcac
     7321 cgaggtcgag ctgctgacgt gcttcatgat ggggcacgac ttctggccga gggaacggtg
     7381 cgtcgccacg ctcgcccgca ttcgtgagct gttccccaac gtcaagcgct tcctgctcgg
     7441 cgacgcgacg aggacggtcg gcaccgccga tcaggagctc cccgtcttca ccctcggctt
     7501 cgaggtggcc cacgacatga tgggcgtctt cctccccacc gtcgccgact gggagtccgc
     7561 gttcgcggaa agcggttggc gggtgcggca caagcacgcg atcgacatga cggtcggcga
     7621 ggtcatcttc gaactcgagc ccgcgtgacc tgacccccgc agaccgttcg acgcacacca
     7681 cacaccgtca tacaactacg ctccgagaag gtggcacgat gcccgatccg cacgacgcaa
     7741 ggaccttccc ggtgacggtt gcccaagagg gtctgttgtt tcatgcggaa gtcgagcggg
     7801 acctacgggg tctcgacacg acgcaggtcg tgctcactgt ggacggtcac ctggacgtgg
     7861 aggcgctcca catccgtgcc gcccgggtcg ccggggcaca tcccggactg ctcgcccgct
     7921 tcgaccgcga tgccctgggc ggacccgtgt gcgtcgcccg tcccggagcc cgcctgcctt
     7981 tggtgcagcg gcacgtcccg gccggcgtcg aggcggacgc gacggcccag cggatggcag
     8041 aagacgaatg ggagcatgcc ttcgacccca ccggggacga acctctggtg cgactggtcg
     8101 tcataacgac cggccaaggc tcacgccacc acctcgtcct caccggccat cgcgcggtgc
     8161 tcacctccga ggccgcccgc gacatcctcg ccgccgtgct cgcgggtgac ccgcgggacg
     8221 ggacgcacgg agcttcgaag gacaccgcgg aagcgggacg gccgtctgaa gcgggaaggc
     8281 cgtcggaggc cgacacgcgc atcgcctggc gggaggcgtt gcgcgacctc gacgaaccct
     8341 gcctgctcgg ccacgcagcc gcgaccgtcc cggacggaga gccgcggcga cgctccgagt
     8401 cgctgtccgt acacgtacca cccgcaatcg gctcgctccc cggccgcctc ggcgttgcac
     8461 ccgaagcgtt ctaccagacc gtctgggctc tggtgtgcat gaagctgaag aaccgtcggg
     8521 aagcggttct cggcgacgcg gacgaccttc ccgttcggat cgtggcggca ccgcacgaca
     8581 cggtcgccga cgtggtccgg cgcaccgacg cgagcaacgg gctcctggcc gtacaccgcc
     8641 atgcgtccgc cgcgacgctg gcggaggcgt cggggctcgc gggcgccgtc ttcgacacgg
     8701 cggtcgtcgt cgacacccgt cgttgggatc gcgcctcgct cgggctcggc gaaccggggg
     8761 aggacgagcc cgcgggcccg gccgtgaccg gcgtacggct gcgcgacacc acgcaccacg
     8821 ccgccactct cgtggtgcgg accgacggcg acggaggacc gcccggcgtc gaactgagcc
     8881 atcgggccga ccggttggca gccgacacgg cccgaacggc cgccgatctg ttcgcccgcg
     8941 ccctggccgt gctggccacc gaaccaggag tgcgagtcgg cgccatcgac tggctcggcg
     9001 attccctgcg ccacgacctg ctcgtcgact acaacgacac gaccgcacgg ctcccgcagg
     9061 cgccgctgca cgagctgttc accgaacagc gggaccggac gccggacgcc gtggccgtcg
     9121 tctgcgaggg gcgtgccctc acctacgccg gtctcgacac ggccgcgaac cggctggccc
     9181 acctcatcat cggccatgga gtgaggccgg gcgacaacgt ggccctctac ctgagccgct
     9241 ccgccgaact gatcatcgcc gaactggccg tgctcaaggc cggcgcggcg tatgtgccgc
     9301 tcgacccccg ccagcccgca cagcgcctct cctggatcgt cacggatgcg ggagcgaccc
     9361 tgttgctcac cgaccgacag ccggccgatc tgccgttcac ctgtgacatc ccatgggctc
     9421 gcgtcccggc gggcgaagac gcggatgctg tgggcgaccc cggagcaccg gacgtcgacg
     9481 tacaccccga tcaactggcg tacgtgatgt acacctcggg ctccaccggt acgcccaagg
     9541 gcgtcgccaa cacgcatcgc aatgtggtcg agctcgcgct cgacccctgg tgggacgcgg
     9601 cacggcacca ccgagtgctc gcctactcgc cgcccgcctt cgactcttcg acctacgaga
     9661 tgtgggtgcc gctgctgcac ggcggtggtc tgacggtcct gaccggtgac aagatcgacc
     9721 tcggagatct ggccggagcc gtcgcggagc acggcatcac cgccctctac ctgaccaccg
     9781 ccctgttcga cgccatggca caggagcacg tcgaggccct ctccgccctc gacgagatct
     9841 ggaccggcgg cgacgtgctg tcgaccacgg ccctgcgccg ggtcctcgac gcctgcccgc
     9901 agaccaccgt cgtccacgtc tacggtccga cggagacgac cgtcttctgc agctaccaga
     9961 cgttcgcccc tggggagcgc ccggtcgagt cgctccacct gggccgtccc atggccaaca
    10021 ccgcgctcta cgtcctcgac gagtcgctcg cccccgctgc ccctggtgtg gtgggtgagc
    10081 tttatgtggc ggggtcgcat ttggcgcggg gttatgtggg gcgtgcgggt ttgacggcgg
    10141 agcggtttgt ggct
//
