LOCUS       NZ_BMUU01000028        10146 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Streptomyces xanthochromogenes strain JCM 4594 sequence28, whole
            genome shotgun sequence.
ACCESSION   NZ_BMUU01000028
VERSION     NZ_BMUU01000028.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces xanthochromogenes
  ORGANISM  Streptomyces xanthochromogenes
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 30459)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650355.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:28:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,827
            CDSs (total)                      :: 7,752
            Genes (coding)                    :: 7,642
            CDSs (with protein)               :: 7,642
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 24 of 110
            Pseudo Genes (incomplete)         :: 94 of 110
            Pseudo Genes (internal stop)      :: 7 of 110
            Pseudo Genes (multiple problems)  :: 12 of 110
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:05:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 20313
            Orig. end                         :: 30459
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUU01000028.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10146
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10146
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10146
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5918](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5918
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            347..1618
                     /db_xref="GeneID:96295564"
                     /locus_tag="IE233_RS37920"
                     /old_locus_tag="GCM10010326_77430"
     CDS             347..1618
                     /codon_start=1
                     /db_xref="GeneID:96295564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003968360.1"
                     /locus_tag="IE233_RS37920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_77430"
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="WP_161245805.1"
                     /transl_table=11
                     /translation="MTHARSASAEAAVAVAVDTEAVRLPAVGETAAGESNSVLEERARR
                     MRRRLERLIGIAATEGNSLTPLRNGDEIFSAMLDGINSARHTVDMMTFVYWKGDIAQRF
                     ADALAERARQGVRVRLLLDGFGSRLIEKEQLDLMERAGVRVAWFRKPLYLSPLKQNHRC
                     HRKVLVVDEETAFTGGVGIAEEWCGDARNPREWRDTHVQVRGPAVDGLAAAFAQNWAEC
                     HEELFDERDRFASHVPQGDAVVQVVRGSASFGWQDMQTLFRTVIESAEERIRMATAYFA
                     PDVYFIELLCAAARRGVEVEILLPGPYTDKRVCQLAGQHYYEDLTACGVKIFEYQPTML
                     HTKVVTVDRTMALVGSTNFNRRSLDHDEEVMLAVLDQTFTAKLDGHFTEDLEHSALITR
                     GRWKRRSIVQRAKEAAVLPIRRFL"
     gene            complement(1804..2703)
                     /db_xref="GeneID:96295565"
                     /locus_tag="IE233_RS37925"
                     /old_locus_tag="GCM10010326_77440"
     CDS             complement(1804..2703)
                     /codon_start=1
                     /db_xref="GeneID:96295565"
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012381901.1"
                     /locus_tag="IE233_RS37925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_77440"
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_229893122.1"
                     /transl_table=11
                     /translation="MDHTKADMGAHRARTGVCVMRFNLIGSFEITTDDGETFAPKAPKL
                     CQILAVLALQPSEAVGSDILIRELWGASSPSGAPRTLQTHIYHTRKMLREADITSPQRE
                     LLLTQAPGYRLLASEDEIDVCLFERLVRSAQSDLAQGAPERASADLKRAMALWRGPLLS
                     NVPTGDVLAGRIAHVEELRIRALELHVQTENALGRYRELLPELRTLVNDYPLHEWFHEQ
                     LIRTLHLAGRRGEALKAYQHLYYLLAQELGLAPSEEIQRLQGEILAVNSVDVPQLRRPR
                     ESGPVGGPAPFWAANVAS"
     gene            complement(2799..3953)
                     /db_xref="GeneID:96295566"
                     /locus_tag="IE233_RS37930"
                     /old_locus_tag="GCM10010326_77450"
     CDS             complement(2799..3953)
                     /codon_start=1
                     /db_xref="GeneID:96295566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018891164.1"
                     /locus_tag="IE233_RS37930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_77450"
                     /product="transposase"
                     /protein_id="WP_161245806.1"
                     /transl_table=11
                     /translation="MRRHQLHPADTNGESVLATGLGAELSARLFESLQRSGQRLNAEQY
                     VRGLLTLPGRKTLRNIAAQFDGVAAQQRVHHFISTSPWDWMPLRRDLADYARQTLMPDA
                     WVMRPTVIPKSGLHSIGVGQHYLPDVGRLVNGQQAVGTWLASAQAALPVDWRLRLPERW
                     LESPLRQRANIPDGAMPGTLEECVGEAVTEVAAAGNPPHCPVVVDADGVDGVALVRRLS
                     TLGIQSLVRVHPDTQLRLDRTALPKYGDGERAAGELTTSLTRLRQRVNPGNSRTTASVI
                     PVVAASSSRGASLLLIRRWSPAGLPDERLWLTPAGTSSLASVLALTRLPDIVARDFAAV
                     SERVGMRDFAGRSYPGWHRHITLACIAHLVAVLGPGDTPRSTRG"
     misc_feature    complement(3903..3905)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(3936..3938)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(4213..4860)
                     /db_xref="GeneID:96295567"
                     /locus_tag="IE233_RS37935"
                     /old_locus_tag="GCM10010326_77460"
     CDS             complement(4213..4860)
                     /codon_start=1
                     /db_xref="GeneID:96295567"
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047020.1"
                     /locus_tag="IE233_RS37935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_77460"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_161245807.1"
                     /transl_table=11
                     /translation="MSRQERSIRTRAAILEAAARLFNEFGYEATTIGVLTERIGLTRGG
                     LYFHFTSKEQLARGVLDEAVTVEGLRPQALKLQEWVDLALLIAYRLPREPILGAAIQLS
                     VDPTVRGLFGTRWPDWIALQTEMLAEAKQRGEVLPHIDPATLARLFVGAWTGVQLVTGT
                     LENRTLPDEISSLLDLLLPSVASDAVRTTLDTSPSRAVHLLAQARGSGDTCD"
     misc_feature    complement(4998..5018)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.92"
                     /tool="antismash"
     gene            5001..5918
                     /db_xref="GeneID:96295568"
                     /locus_tag="IE233_RS37940"
                     /old_locus_tag="GCM10010326_77470"
     CDS             5001..5918
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96295568"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="IE233_RS37940"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_77470"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_267907228.1"
                     /sec_met_domain="AfsA (E-value: 1.4e-17, bitscore: 54.4,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAATYCLEDPCARSVARQTSPLHEYAHLRDESAMFVTGYRRIAE
                     HEFRLCASWPASPGELAYEPRVLVQTVRQCGLIVAHAEYGVPLTHQTLLHNMNFTMRPA
                     LHDFRDAPSSLDIRTVVSDTRRPGRATDMLRMDFQILRAGAVVAGADLEFAWISPRAYR
                     RLRGAQLDVVWGDWPLPAPADPGLVGRSTDDDVVLAADGAPNRWLLRNDIRDRMLFDHA
                     VDHVPGAVLLEAADQAARALLAPRHFVPSGLTSNFSQYVEFDSPCSIRAEPLPDPGHNQ
                     VAVLISGTQDGESAFTVELTGTTC"
     misc_feature    5022..5024
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(5971..6735)
                     /db_xref="GeneID:96295569"
                     /locus_tag="IE233_RS37945"
                     /old_locus_tag="GCM10010326_77480"
     CDS             complement(5971..6735)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (9-169). E-value: 1e-12. Score:
                     40.3. Matches aSDomain:
                     nrpspksdomains_IE233_RS37945_PKS_KR.1"
                     /codon_start=1
                     /db_xref="GeneID:96295569"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014047022.1"
                     /locus_tag="IE233_RS37945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_77480"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_190029621.1"
                     /sec_met_domain="adh_short (E-value: 3.9e-38, bitscore:
                     122.0, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 7.4e-34, bitscore:
                     108.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSDLLLNKVVLITGASSGIGEAAARVFAQEGAIVVLTARRADRL
                     AALVGELRDKGAEASCVVGDVSVADDMAKVVDFAVTEYGRLDSAFNNAGIGGDRSPLHL
                     MSDDVYDTIMDINVRGVFNCMRHEIAAMLPHGGGSIVNNSSVGGLVSIPTAAPYIASKH
                     AVVGLTRAAADEYAKQGIRVNAIAPGTTRSEITADWFGRNPGLEELVNSATPQGRTAHP
                     DEIAAAAAWLLSDRCPFLTGTVLPVDGGYVNQ"
     aSDomain        complement(6229..6708)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS37945_PKS_KR.1"
                     /evalue="1.00E-12"
                     /label="IE233_RS37945_PKS_KR.1"
                     /locus_tag="IE233_RS37945"
                     /protein_end="169"
                     /protein_start="9"
                     /score="40.3"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VVLITGASSGIGEAAARVFAQEGAIVVLTARRADRLAALVGELRD
                     KGAEASCVVGDVSVADDMAKVVDFAVTEYGRLDSAFNNAGIGGDRSPLHLMSDDVYDTI
                     MDINVRGVFNCMRHEIAAMLPHGGGSIVNNSSVGGLVSIPTAAPYIASKHAVVGLT"
     CDS_motif       complement(6613..6708)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS37945_0001"
                     /evalue="3.00E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE233_RS37945"
                     /protein_end="41"
                     /protein_start="9"
                     /score="13.5"
                     /tool="antismash"
                     /translation="VVLITGASSGIGEAAARVFAQEGAIVVLTARR"
     misc_feature    6709..6722
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 16.22"
                     /tool="antismash"
     misc_feature    6711..6724
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.65"
                     /tool="antismash"
     misc_feature    complement(6715..6717)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(7123..7539)
                     /db_xref="GeneID:96295570"
                     /locus_tag="IE233_RS37950"
                     /old_locus_tag="GCM10010326_77490"
     CDS             complement(7123..7539)
                     /codon_start=1
                     /db_xref="GeneID:96295570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015610882.1"
                     /locus_tag="IE233_RS37950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_77490"
                     /product="DUF4440 domain-containing protein"
                     /protein_id="WP_189766501.1"
                     /transl_table=11
                     /translation="MAITVDIDFDNPDLTDDTDLQNEIFLQVFNSGDGELFDKLYRADA
                     ISNFSGEPLTGAARLKFFKEFLASKPSLQATVTHSYVAGDVALIGVQYTIDGIQADGAP
                     LHLEGTCTDVLRRVEDGRWLMAVDRPVAGSLLPE"
     gene            complement(7692..8069)
                     /db_xref="GeneID:96295571"
                     /locus_tag="IE233_RS37955"
                     /old_locus_tag="GCM10010326_77500"
     CDS             complement(7692..8069)
                     /codon_start=1
                     /db_xref="GeneID:96295571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017946701.1"
                     /locus_tag="IE233_RS37955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_77500"
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_161245703.1"
                     /transl_table=11
                     /translation="MTQLTSDVDKHTALYVEAFNSGDADAVNAMYTEEAVAVWEPGVAL
                     TGKARIDSVKEFLAAGPKMTAVVRQSFVTGDTALLIVDWKIDTTDADGKPEHLTGVGVD
                     VLRLGTDGHWRYAIDDPYGEK"
     gene            8448..>10146
                     /db_xref="GeneID:96295572"
                     /locus_tag="IE233_RS37960"
     CDS             8448..>10146
                     /NRPS_PKS="Domain: PKS_Docking_Nterm (3-31). E-value:
                     1.4e-11. Score: 35.7. Matches aSDomain:
                     nrpspksdomains_IE233_RS37960_PKS_Docking_Nterm.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (35-462). E-value:
                     2.4e-181. Score: 595.2. Matches aSDomain:
                     nrpspksdomains_IE233_RS37960_PKS_KS.1"
                     /codon_start=1
                     /db_xref="GeneID:96295572"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008742168.1"
                     /locus_tag="IE233_RS37960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_190029622.1"
                     /sec_met_domain="mod_KS (E-value: 9.9e-229, bitscore:
                     750.0, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 2.2e-93, bitscore:
                     303.2, seeds: 77, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAGEDQLRDYLRRAIADARDARSRLQEMQDRQREPIAIVSMACRF
                     PGGVASPEDLWQLVSEGVDAVTPFPENRGWDLDGLYDPDPNTPGTSYSREGGFLHDADR
                     FDASFFEMSPREALTTDPQQRLLLQTAWEAFERAGIDPKALRGSRTGVFTGIMYNDYGS
                     RPGLPAENTEGYLFSGSAGSIASGRLSYTYGLEGPAVTVDTACSSSLVALHLAANALRR
                     GECDLALAGGATVMSTPVAFVEFSRLRGLAPDGRIKSFGAGADGTAWSEGVGLLLVERL
                     SDARRHGHEVLAVVRGSAVNQDGASNGLTAPNGPSQERVIRQALADAGLVEADVDVVEA
                     HGTGTRLGDPIEAQALLATYGRGRSAERPLYLGSLKSNIGHAQAASGVGGVIKMVQAMR
                     HGMLPRTLHAEEPTPMVDWSSGTIELLTRERDWPASDARARRASVSSFGFGGTNAHVIL
                     EEAPSEVTPAEAESAPDKVDTALENVPADQAPAPRIPVVPWVLSGRSVEALRGQAGRLL
                     GLLGGGSGLSDLDVGFSLATSRAVHEFRAVVVVGGDRRGALEALAVGGG"
     aSDomain        8457..8540
                     /aSDomain="PKS_Docking_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS37960_PKS_Docking_Nterm.
                     1"
                     /evalue="1.40E-11"
                     /label="IE233_RS37960_PKS_Docking_Nterm.1"
                     /locus_tag="IE233_RS37960"
                     /protein_end="31"
                     /protein_start="3"
                     /score="35.7"
                     /tool="antismash"
                     /translation="EDQLRDYLRRAIADARDARSRLQEMQDR"
     aSDomain        8553..9833
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS37960_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="2.40E-181"
                     /label="IE233_RS37960_PKS_KS.1"
                     /locus_tag="IE233_RS37960"
                     /protein_end="462"
                     /protein_start="35"
                     /score="595.2"
                     /tool="antismash"
                     /translation="IAIVSMACRFPGGVASPEDLWQLVSEGVDAVTPFPENRGWDLDGL
                     YDPDPNTPGTSYSREGGFLHDADRFDASFFEMSPREALTTDPQQRLLLQTAWEAFERAG
                     IDPKALRGSRTGVFTGIMYNDYGSRPGLPAENTEGYLFSGSAGSIASGRLSYTYGLEGP
                     AVTVDTACSSSLVALHLAANALRRGECDLALAGGATVMSTPVAFVEFSRLRGLAPDGRI
                     KSFGAGADGTAWSEGVGLLLVERLSDARRHGHEVLAVVRGSAVNQDGASNGLTAPNGPS
                     QERVIRQALADAGLVEADVDVVEAHGTGTRLGDPIEAQALLATYGRGRSAERPLYLGSL
                     KSNIGHAQAASGVGGVIKMVQAMRHGMLPRTLHAEEPTPMVDWSSGTIELLTRERDWPA
                     SDARARRASVSSFGFGGTNAHVILEEAP"
     CDS_motif       9036..9083
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS37960_0001"
                     /evalue="2.30E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE233_RS37960"
                     /protein_end="212"
                     /protein_start="196"
                     /score="22.5"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       9459..9503
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS37960_0002"
                     /evalue="7.20E-05"
                     /label="PKSI-KS_m5"
                     /locus_tag="IE233_RS37960"
                     /protein_end="352"
                     /protein_start="337"
                     /score="14.6"
                     /tool="antismash"
                     /translation="VEAHGTGTRLGDPIE"
     CDS_motif       9564..9608
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS37960_0003"
                     /evalue="1.00E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="IE233_RS37960"
                     /protein_end="387"
                     /protein_start="372"
                     /score="11.6"
                     /tool="antismash"
                     /translation="GSLKSNIGHAQAASG"
ORIGIN
        1 ctattcccac cggcctcacg gcaccgctcc cggccagccc gcctacgtca caactccaca
       61 gcggtgtcac caacgtgccg gtctccccgg cctggcgctt gttggccgag cccgcaatca
      121 ggtggagctg ctggtgcatg gcggcggctg aagtggacag gaggcgatgg gcgccgatgc
      181 agccagcaac tccgggtgta tcggactcgt cgaagcgggg gttcctggat cggctggatg
      241 tgtgaaccgt gcaggcctct tgagtcggct gtccggcacc tccggcgatc ctggctacgg
      301 ccgtcctggt ttgtcctctt ggtcagcgtt tggggaaggt tcgaacatga cgcatgcgcg
      361 cagtgcttcc gcggaagcgg cggtagcggt ggcagtagac actgaggctg tccggctgcc
      421 ggcggtcggt gagaccgctg ccggggagtc gaactcggtt cttgaggaac gagcccggcg
      481 tatgagacgc cgactggaga gactcatagg gattgccgcg accgagggca attccttgac
      541 gccgttgcgc aatggtgacg agatcttctc cgccatgctg gacgggatca attccgcccg
      601 gcacacagtc gacatgatga cattcgtcta ctggaagggc gacatcgctc agcggttcgc
      661 ggatgccctc gcggagcggg cgcggcaggg agtgcgggtg cggctccttt tggatgggtt
      721 cgggagccgg ctcatcgaga aggagcagct ggacttgatg gagcgtgccg gagtgcgggt
      781 ggcgtggttc cgtaagccgc tctatctgtc gccgctgaag cagaaccacc gctgtcaccg
      841 caaagtcctg gtcgttgacg aggaaacggc gttcaccggt ggtgtcggca ttgccgagga
      901 gtggtgcgga gatgcccgca atccgcgtga atggcgcgac actcatgtcc aggtgcgggg
      961 gccggccgtg gacgggctgg ctgccgcgtt cgcgcagaac tgggctgagt gccacgagga
     1021 actcttcgac gagcgcgacc ggttcgcctc ccacgttccc cagggcgacg ctgtcgtgca
     1081 ggtggtgcgc ggctccgcca gcttcggctg gcaggacatg cagactctgt tccggacagt
     1141 gatcgagtcg gcggaggaac ggatccggat ggccacggcc tacttcgccc ccgacgtgta
     1201 cttcatcgag ctgctgtgcg cggccgcccg tcgcggggtc gaggtcgaga ttctgctgcc
     1261 agggccctac accgacaagc gcgtctgcca attggccggg cagcactact acgaggacct
     1321 gaccgcctgc ggagtcaaga tcttcgagta ccagccgacg atgctgcaca ccaaagtcgt
     1381 caccgtcgac cggactatgg ctctggtcgg ctcgacgaac ttcaaccgcc gctcgctgga
     1441 ccacgacgaa gaagtgatgc tcgctgtgtt ggaccagacg ttcacggcga agctggacgg
     1501 ccacttcaca gaggacctgg aacacagcgc actcatcacg cgaggacgct ggaagcgacg
     1561 ctccatcgtt cagcgcgcca aggaggccgc agtcctcccg atccgccgct ttctgtgagc
     1621 gggggtgacc gattcccctt ggccatgtgc acaggagcga atactgctgc ttcatcagca
     1681 gcacgccgac accgttgagg cccacgtcgt cggcagcccg gggcggaatg ccactccggg
     1741 ccccgaggac ggcagagccg gggccaggca actcgcggtg ctcggggccc gggatgctca
     1801 gcctcaactg gcgacgttgg ccgcccagaa gggggcgggg ccgccgaccg ggcccgattc
     1861 ccgcgggcgg cgcagttgcg gcacgtccac ggagttcacc gcgaggatct cgccctggag
     1921 ccgttggatc tcctcggagg gcgccagacc cagttcctgg gcgagcaggt agtagaggtg
     1981 ctggtacgcc ttgagggcct cgccacgacg gccggcgagg tgcagggtcc tgatgagctg
     2041 ttcgtggaac cactcgtgca aggggtagtc gttcacgaga gtgcgcagct cgggcagcaa
     2101 ctcccggtac cggccgagcg cgttctccgt ctggacgtgc aactcgagtg cacggatacg
     2161 gagttcctcg acgtgtgcga tccggccggc gagcacgtct cccgtgggga cgttgctcag
     2221 cagggggccc cgccacagcg ccatcgcgcg cttgaggtcg gcggaggcgc gctccggtgc
     2281 gccctgggcg agatcgcttt gagcgctgcg gacgagccgc tcaaaaaggc agacgtcgat
     2341 ctcgtcctcg gacgcgagca ggcggtaccc cggcgcctgc gtgagcaaca actcccgctg
     2401 cggcgaggtg atatcggcct cacggagcat tttgcgggtg tggtagatgt gcgtttggag
     2461 ggttctcggg gcgccgctgg gggagctcgc cccccagagc tcgcggatca ggatgtccga
     2521 gcccaccgcc tcggacggct ggagagccag aacggcgagg atctggcaga gtttcggcgc
     2581 cttcggggcg aacgtctcac cgtcatcggt ggtgatctcg aacgagccaa tgaggttgaa
     2641 ccgcatcacg cacactcccg tcctggcgcg gtgggcgccc atgtcggcct tggtgtgatc
     2701 caaacgtaaa gaagaggctc ccttgacctg gcatgtgcat gggcgagagt gtcaagttac
     2761 cggatggtaa ttaggtgttt cggggatgcg ggtcaccgtc agcccctcgt actccgcggg
     2821 gtgtcgcccg gccccagcac ggccaccagg tgcgcgatgc aggccaacgt gatgtggcga
     2881 tgccagccgg gatacgaccg gcccgcgaag tcgcgcattc ccaccctctc ggagactgcc
     2941 gcgaagtccc gcgcgacgat gtccggcagc cgggtgagcg cgagaacgga tgccagcgac
     3001 gacgtgcccg cgggcgtcaa ccagaggcgt tcgtccggta gtccggccgg ggaccatctt
     3061 cgtatcagga gcaatgacgc gccccgtgag gacgaggccg cgacgaccgg aatgaccgag
     3121 gccgtggtgc ggctgttgcc gggattcacc cgctgacgca ggcgggtcag ggaggtggtg
     3181 agctcaccgg cggcgcgctc gccgtccccg tacttgggca acgccgtccg gtcgagccgt
     3241 agctgcgtgt ccgggtgcac gcgcaccagg gactggatgc ccagcgtgga gagacgccgg
     3301 accagcgcca ccccgtccac cccgtccgcg tcgaccacga cgggacagtg cggggggttg
     3361 ccggcagccg cgacctcggt caccgcttcg cctacgcact cctcgagcgt gccgggcatg
     3421 gcgccgtccg gaatgttcgc acgctgcctc aggggactct ccagccaccg ctcgggcagc
     3481 cgcagtcgcc agtcgaccgg cagcgcggcc tgggcggagg cgagccaagt ccccaccgcc
     3541 tgctggccgt tgacgaggcg accgacgtcc ggcaggtagt gctggcccac cccgatcgaa
     3601 tgcagaccgg acttggggat cacggtgggc cgcatcaccc acgcgtcggg catgagggtc
     3661 tgtcgcgcgt agtcggcgag atcgcgccgg agcggcatcc agtcccacgg tgacgtgctg
     3721 atgaaatggt ggacccgctg ctgcgccgcg acaccgtcga actgtgccgc gatgttgcgc
     3781 agtgtcttgc gccccggcag cgtgagcagc ccccgtacgt attgctccgc gttcaagcgc
     3841 tgtccgctgc gctgcagcga ctcgaagagc cgcgcgctca gttccgcgcc gaggccggtc
     3901 gctaagaccg actccccgtt ggtgtcggcc gggtgtaact gatgccgcct catggttcct
     3961 tccgtatgcc tggccggtag tgccgccacc gccccgtccc cccgcgatac gcgaaatgcc
     4021 ctcgccccgg cccggcaaag gacgcaggtc ctcactaaaa aggacgtccg ttcggtagtt
     4081 tcggtgagac cgtatcgtgt ggcggcacgc tctccgtccc tgcgcggtgt ggtacccgcg
     4141 tcgtggcggt gcccgctccg ccgcggcgta cgcggcgagc ggtcgggcgg tggtgtgggt
     4201 gtggcagcct cttcagtcgc acgtgtcccc tgagccacgt gcctgcgcga ggaggtgtac
     4261 ggcacgggac ggggaggtgt cgagcgtcgt ccgtacggcg tcactcgcga cactggggag
     4321 caacaggtcg agcaggctgg agatctcgtc gggaagtgtg cggttctcca aggtgcccgt
     4381 gaccagttgc acaccggtcc aggctccgac gaacagccgg gcgagagtcg ccggatcgat
     4441 gtgcgggagc acctcgcccc gctgtttcgc ctccgcgagc atctcggtct gcagggcgat
     4501 ccaatccggc cagcgcgtgc cgaacaggcc gcggaccgtg gggtcgacgg acagttggat
     4561 ggcggcgccg agtatgggct ccctgggcag gcggtaggcg atgaggagtg ccaggtcgac
     4621 ccactcctgc agcttgaggg cctgcggacg caggccctcg acggtcaccg cctcgtcgag
     4681 cactccccgg gccagttgct ccttcgacgt gaagtggaag tacaggccgc cccgcgtgag
     4741 tcctatgcgc tcggtgagca cgccgatggt cgtggcttcg tagccgaact cgttgaacag
     4801 acgggccgcc gcctccagga tggccgcacg ggtgcgaatc gatctttcct gcctcgacac
     4861 tcacaactcc cttgactagc tctttaccag catgtgaagt tcaccctggc cgtccccgaa
     4921 acctaacggt ggtccggtac tttacaagtt gatcaaggtt ttcccagctc atcaccgaag
     4981 ccaggggagt tctgccgaac atgtccgcag ctacgtattg cttagaagac ccgtgcgccc
     5041 gttccgtcgc gcggcagacg tctccactcc atgagtacgc ccatctcaga gacgagagcg
     5101 ccatgttcgt cacgggctac aggcgcatcg cggagcacga gttcagactc tgtgccagtt
     5161 ggccggcctc gccgggcgag ctggcctacg aaccccgggt actcgtccaa acggtccggc
     5221 agtgcggtct catcgtcgcc catgccgaat acggcgttcc gctgacgcat cagacgctgc
     5281 tgcacaacat gaacttcacg atgcggcccg ctctgcacga cttccgtgac gcgccctcca
     5341 gcctggacat ccgcacggtc gtgtccgata cgagacggcc gggacgcgcc accgacatgc
     5401 tcaggatgga cttccagatc ctgcgggccg gtgccgtcgt ggccggcgcg gacctggagt
     5461 tcgcctggat atccccccgg gcctaccgac ggctgcgcgg ggcccaactc gacgtggtgt
     5521 ggggggactg gccgctgccg gcgcccgccg acccagggct cgtcggccgg tccacggacg
     5581 atgatgtggt cctcgcggcg gacggcgcac cgaaccgctg gctactgcgc aacgacattc
     5641 gcgaccgcat gctcttcgac cacgccgtcg accacgtgcc gggagcggtc ctgctggagg
     5701 ccgccgacca ggccgcccgt gcgctgctgg cgccgcggca cttcgtgccc tccggcctca
     5761 cctcgaactt ctcgcagtac gtggagttcg actcgccctg ctcgattcgg gccgagccgc
     5821 ttcccgatcc cggccacaac caggtcgccg tcctgatcag cggtactcag gacggcgaga
     5881 gcgcgttcac cgtggaactc accggcacga cctgctgacg gccgcccctg ccccggccgg
     5941 gggccactgt ccccggccgg gacggtgccg tcactggttg acgtagccgc cgtccaccgg
     6001 cagcacggtg ccggtcagga acgggcaccg gtcgctgagc agccaggcgg ccgcggccgc
     6061 gatctcgtcg ggatgggccg tacgtccttg cggagtagcg gagttgacca gttcctcaag
     6121 gccgggattg cggccgaacc agtcggcggt gatctcgctg cgcgtggtgc cgggggcgat
     6181 cgcgttcacg cggatgccct gcttggcgta ttcgtcggcc gccgccctgg tgaggccgac
     6241 gaccgcgtgc ttcgaggcga tgtagggggc ggccgtgggg atcgacacca ggccacccac
     6301 gctgctgttg ttgacgatcg agccgccgcc gtgaggcagc atcgcggcta tctcgtgccg
     6361 catgcagttg aagactcccc gcacgttgat gtccatgatg gtgtcgtaga cgtcgtcgga
     6421 catcaggtgc agggggctgc gatcaccgcc gatccccgcg ttgttgaagg ccgagtcgag
     6481 ccgcccgtac tcggtgacgg cgaagtcgac aaccttggcc atgtcgtcgg cgacggacac
     6541 gtcaccgacc acgcaggacg cttcggcccc cttgtcgcgc agttcgccga ccagggcggc
     6601 cagccgatcc gctctgcggg cggtgaggac cacgatcgcg ccctcctgcg cgaacacccg
     6661 tgccgccgcc tccccgatgc cgctgctggc acccgtgatg agcactacct tgtttaaaag
     6721 aagatcactg ttcacagcgg aagtgtggca tgcgcaggta cccggcccgc gggcggaact
     6781 gtgcttcgcc gcccggcact tggccgctgc tgcccggcgg ggtggccccg acccgtgccc
     6841 gccacggcca tcacctgagc ggtccctcgg ctgcgacgcg gttcaagcgg cccccgctcc
     6901 actggcgccc cgggcccgac cagccctagg accgcgacgg gcgtgcgggc agcagccgcg
     6961 ctgtcccgag gcgcgcagca catgaggcgc ggcggaggga atcgcccctc catctcacgg
     7021 caacagccgg ttcatcgacg ccggaagggc ggaccctccc gcgccgggcg gtactcgctc
     7081 cggcgcggga gacccgtccg cgtccatggc gggagcgcgt gcctactccg gcagcaggct
     7141 gcccgcgacc ggcctgtcga cggccatgag ccagcgcccg tcctcgacgc ggcgcagcac
     7201 gtcggtgcag gtcccctcca ggtgcagggg cgcaccgtcg gcctggatgc cgtcgattgt
     7261 gtactgcacg ccgatcaggg ccacgtcgcc ggccacataa gagtgggtga ccgtcgcctg
     7321 gagcgaaggc ttcgaggcca ggaattcctt gaagaacttc agccgagccg ctccggtcag
     7381 cggctcgccc gagaaattgg atatcgcatc ggcgcgatag agcttgtcga acagctcgcc
     7441 gtcacccgag ttgaacacct gcaggaagat ctcgttctgc aggtcggtgt cgtcggtcag
     7501 atccgggttg tcgaaatcga tgtcgacagt gatggccatg gagatgcaga ccctacttcc
     7561 tcattgactg gacaggttgg gcaccgctcg tcccggaggc gggttctttc ccctccggca
     7621 gctgtcggtg ccttgctcat cgtcgagcgt ctcgcgcacg ggacggatgg ttccgcgtcc
     7681 ccgcgctcaa ctcacttctc gccgtacggg tcgtcgatgg cataccgcca gtggccgtcc
     7741 gtaccgagcc gcagcacgtc gacaccgacg ccggtgaggt gctccggctt gccgtcggcg
     7801 tccgtggtgt cgatcttcca gtccacgatc agcagagcgg tgtcgccggt cacgaaggac
     7861 tgtcggacca cggccgtcat cttcggcccc gcggcgagga attccttgac cgagtcgatc
     7921 ctggcctttc ccgtgagcgc gactcccggc tcccacacgg ccacggcttc ttcggtgtac
     7981 atcgcgttga ccgcatccgc gtcgcccgaa ttgaatgcct cgacgtagag tgcggtgtgc
     8041 ttgtcgacgt cactggtgag ttgcgtcatg ctgagacatc tccctttcct gttcccgccg
     8101 gacagaacgg cattccgagt ccggtggacc cggcagagaa taccgatggg gaaacaacca
     8161 catgctcatt gcgaagtaat acagcggact cggcccgtcg ctctccgcac gattaccgag
     8221 gtgtggttga cccgtcgtta cgatcaccca ctcacgctgg tattcgccgc gtcgcacggc
     8281 tacgcgaaag gccgacgccg cgcatcgcgg acaagggcag tcggcgcgcg accacgtccg
     8341 tccaccgcac tggccgacga acctcacggc ctgcgatcac cacgacgcac gaacaccacg
     8401 aagcacgaac accacggccc accaacacca cacagggagc tgagcacatg gccggcgagg
     8461 accaactccg ggactacctc aggcgagcca tcgcggacgc ccgcgacgcg cggagccggc
     8521 ttcaagagat gcaggacagg cagcgtgagc cgatcgcgat cgtctcgatg gcgtgccgat
     8581 tccccggtgg ggtggcctcg ccggaggatc tgtggcaact ggtgagcgag ggagtcgatg
     8641 ccgtcacccc cttccccgag aaccggggtt gggacctcga cgggctctac gaccccgacc
     8701 cgaacacacc cggcaccagc tacagccggg agggcggctt cctccatgac gccgatcgtt
     8761 tcgacgcttc gttcttcgag atgtcgccgc gcgaggccct gaccaccgac ccgcagcagc
     8821 gcctgctcct gcagaccgcg tgggaggcgt tcgagcgggc cggcatcgac cccaaagcac
     8881 tccggggcag ccgtaccgga gtgttcaccg gcatcatgta caacgactac ggctcccggc
     8941 ctggcctgcc tgccgagaac accgagggct atctgttcag cggcagcgcg ggcagcatcg
     9001 cctccggccg gctctcctac acatacggtc tcgaaggccc cgccgtcacc gtcgacacgg
     9061 cctgctcgtc ctccctcgtg gccctgcacc tggccgccaa cgcgctgcgc cgcggcgagt
     9121 gcgatctcgc gctcgccgga ggcgcgaccg tgatgtccac accggtcgcg ttcgtggagt
     9181 tctcccgact gcgtggtttg gcgccggatg ggcggatcaa gtcgtttggg gcgggggcgg
     9241 atggtacggc gtggtcggag ggggtggggt tgttgttggt ggagcggttg tcggatgcgc
     9301 gtcggcatgg gcatgaggtg ttggcggtgg tgcgtggttc ggcggtgaat caggatggtg
     9361 cgtcgaatgg tttgactgcg cccaatggtc cgtcgcagga gcgggtgatt cggcaggcgt
     9421 tggcggatgc ggggttggtt gaggcggatg tcgatgtggt ggaggcgcat ggtacgggta
     9481 cgcggttggg tgatccgatc gaggcgcagg cgttgctggc gacgtatggg cgtggtcgtt
     9541 ccgctgaacg gccgctgtat ctgggctcgt tgaagtccaa catcggacac gcacaagctg
     9601 cttccggcgt cggcggtgtg atcaagatgg tgcaggcgat gcggcacggg atgctgccca
     9661 ggacgctgca cgcggaggag cccaccccga tggtcgactg gtcgtcgggc accatcgaac
     9721 tgctcacccg ggaacgggac tggccggcgt cggacgcccg tgcccggcgt gcgagcgtct
     9781 cctccttcgg cttcggcggc acgaacgcgc atgtgatctt ggaagaggcg ccgtcggagg
     9841 tgacgcccgc cgaggccgaa tcggcaccgg acaaggtcga tacggcgttg gaaaacgtgc
     9901 cggcggatca ggcgcccgcg cctcggatcc cggtggttcc gtgggtgctt tctgggcggt
     9961 cggttgaggc gttgcgtggt caggcggggc gtttgttggg gttgttgggt gggggttcgg
    10021 gtctgtcgga tctggatgtg ggtttttcgt tggcgacgtc gcgtgcggtg catgagtttc
    10081 gtgcggtggt ggtggtgggt ggtgatcgtc gtggggcgtt ggaggcgttg gcggttggtg
    10141 gtggtc
//
