LOCUS       NZ_BMUU01000026        10936 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Streptomyces xanthochromogenes strain JCM 4594 sequence26, whole
            genome shotgun sequence.
ACCESSION   NZ_BMUU01000026
VERSION     NZ_BMUU01000026.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces xanthochromogenes
  ORGANISM  Streptomyces xanthochromogenes
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 34876)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650355.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:28:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,827
            CDSs (total)                      :: 7,752
            Genes (coding)                    :: 7,642
            CDSs (with protein)               :: 7,642
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 24 of 110
            Pseudo Genes (incomplete)         :: 94 of 110
            Pseudo Genes (internal stop)      :: 7 of 110
            Pseudo Genes (multiple problems)  :: 12 of 110
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:05:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 15674
            Orig. end                         :: 26610
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUU01000026.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10936
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10936
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10936
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5936](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5936)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            92..1120
                     /db_xref="GeneID:96295508"
                     /locus_tag="IE233_RS37640"
                     /old_locus_tag="GCM10010326_76880"
     CDS             92..1120
                     /codon_start=1
                     /db_xref="GeneID:96295508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518533.1"
                     /locus_tag="IE233_RS37640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_76880"
                     /product="YdcF family protein"
                     /protein_id="WP_190029600.1"
                     /transl_table=11
                     /translation="MVAFVPSFVCFFLFGVGVVRDRRRVSNAVLLGLAIAFAALALLLH
                     VASEQAQLGRDLVIVLLTLAGVGVVTLAWFLIANGITMVRKEGRSPANLLSLGAGLVLV
                     ALLALLITALVLHTHTLLVVAATAVTLAGYVAFLFLCFLVYGGLYGRLRIRRRVDYVVV
                     LGSGLIGGTTVPPLLAARLDRARKEHARLSRKGRRTLLLTSGGQGPDEKLPESHAMADY
                     LVAQGFPADLIEREDRSTTTDENLKFSKVLMERANPDYRCVIVTNNYHVFRAAITARRT
                     AVRGHVIGAPTAAYFWPSAMIREFVALMVAYRRTNATLFLLVMLSGLFIWWLGWPSPGA
                     VT"
     gene            1648..>2391
                     /db_xref="GeneID:96295509"
                     /locus_tag="IE233_RS37645"
                     /old_locus_tag="GCM10010326_76890"
                     /pseudo=""
     CDS             1648..>2391
                     /NRPS_PKS="Domain: Abhydrolase_1 (29-125). E-value:
                     4.5e-22. Score: 70.8. Matches aSDomain:
                     nrpspksdomains_IE233_RS37645_Abhydrolase_1.1"
                     /codon_start=1
                     /db_xref="GeneID:96295509"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_6"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1262: haloalkane dehalogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018531973.1"
                     /locus_tag="IE233_RS37645"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_76890"
                     /product="alpha/beta fold hydrolase"
                     /pseudo=""
                     /sec_met_domain="Abhydrolase_6 (E-value: 1e-12, bitscore:
                     40.3, seeds: 455, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 7.5e-19, bitscore: 59.5,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRPSAAAASAPRGGSIEISCTIPEAAALGFPQTHLMWRHVAERL
                     ADRHTVFCPDLRGYGASDKPAATDPEVYAKRTMAADIVTLAASLGHERCALVGHDRGAL
                     VAFRAALDHPETITHLGILDIVPMLGMWNVMRGVSAAVGCHLYLVAQPPGLPEAMIAGN
                     ADAFFGSFLGAWANDPTAMPADVRAEYLRASGAAVTSVVADYRASADIDVTHDQADLDA
                     GSQLAMPVTVMQQDWGAQLGFDAAGM"
     aSDomain        1735..2022
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS37645_Abhydrolase_1.1"
                     /evalue="4.50E-22"
                     /label="IE233_RS37645_Abhydrolase_1.1"
                     /locus_tag="IE233_RS37645"
                     /protein_end="125"
                     /protein_start="29"
                     /score="70.8"
                     /tool="antismash"
                     /translation="GFPQTHLMWRHVAERLADRHTVFCPDLRGYGASDKPAATDPEVYA
                     KRTMAADIVTLAASLGHERCALVGHDRGALVAFRAALDHPETITHLGILDI"
     gene            complement(2775..3557)
                     /db_xref="GeneID:96295510"
                     /locus_tag="IE233_RS37650"
                     /old_locus_tag="GCM10010326_76900"
     CDS             complement(2775..3557)
                     /codon_start=1
                     /db_xref="GeneID:96295510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029963.1"
                     /locus_tag="IE233_RS37650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_76900"
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="WP_190029601.1"
                     /transl_table=11
                     /translation="MRVRRLGWAGLEIEAGAKRLVVDYVRDLSPLFTGWKRGECLAAPT
                     GKVDAALVTHLHRDHTDAPALAEVLTPGAPVLRPAPGHGDDVDNVATLLAESELTRHRL
                     AATVIDAWATREIGPFRVTAVPAVDGLGDPQLNWVVEADGQRVFHGGDTMFHGYWWLIA
                     RRFSPFDAAFLPANGPVVDAPHLQPPSPVPAALDPREAAAAAEILDARYVVPIHYEPDQ
                     LDKITGYREVADPAAELREHAGPRARVLAIGEWLDLTA"
     gene            3649..4251
                     /db_xref="GeneID:96295511"
                     /locus_tag="IE233_RS37655"
                     /old_locus_tag="GCM10010326_76910"
     CDS             3649..4251
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96295511"
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010063275.1"
                     /locus_tag="IE233_RS37655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_76910"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_190029602.1"
                     /transl_table=11
                     /translation="MSPRRSVAEAQATKGRILSRAAEIASEEGLDGITIGRLAEALEMS
                     KSGVHKHFGTKESLQISTLDKAFVDFWHRVVEPALGSPPGLRRLRAVCANSVRYLEAPL
                     LPGGCLMTAALTEYDGRPGRVRDAVAEVWSRWQEQLRADLTAAVESAELPAGFDIDQAL
                     FEIIAAGLALNAAMQLQHDRSAADRARLAIERALSQP"
     gene            complement(5001..5936)
                     /db_xref="GeneID:96295512"
                     /locus_tag="IE233_RS37660"
                     /old_locus_tag="GCM10010326_76920"
     CDS             complement(5001..5936)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96295512"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575610.1"
                     /locus_tag="IE233_RS37660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_76920"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_190029603.1"
                     /sec_met_domain="AfsA (E-value: 1.5e-16, bitscore: 51.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVTTGSIMTIESTQRVPMALVRKTVASQALLTAWRRLGDGVSHT
                     VVANWPQTHGFYTSHHELYHPLLLAETIRQALALLSHTAYDIPLDHRLGWESFDTSLIP
                     TALRTRSTPSVVRLTITHTEVSRRRFGSAHLAAQITATRDKEPLGIARIRYTAHPPAIY
                     NRLRGDHADATAATARALPLGPALAPSVRGEQTSLDDAVLSPLGESHRWQLRVNTANRT
                     FFDHDHDHIPGLVFLEAAAQAAHALSGARRTVPMNFDAHFDRYVELDSPCWLEATPLRR
                     RTPGVISTRLTGRQKDSEVFSILVTASEYN"
     misc_feature    complement(6009..6026)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 16.08"
                     /tool="antismash"
     misc_feature    6010..6030
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.67"
                     /tool="antismash"
     misc_feature    complement(6011..6031)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.18"
                     /tool="antismash"
     misc_feature    6019..6032
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 16.24"
                     /tool="antismash"
     misc_feature    complement(6019..6034)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.57"
                     /tool="antismash"
     gene            6060..6674
                     /db_xref="GeneID:96295513"
                     /locus_tag="IE233_RS37665"
                     /old_locus_tag="GCM10010326_76930"
     CDS             6060..6674
                     /codon_start=1
                     /db_xref="GeneID:96295513"
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018565419.1"
                     /locus_tag="IE233_RS37665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_76930"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_161256537.1"
                     /transl_table=11
                     /translation="MVQREPKQERSARTREAILRAGAEVFAEVGFAGASVNKIARRANL
                     TLGALYFHFESKEQLAREIVQAQPTQITLPATVHGLQGAVDTTIAWATQLLNDPMLLAG
                     ARLVMDQEYFVDAEKGNSHQQWVEVLLPFLYEAAQAGELKPDVDVEALARLVVNAATGA
                     QMNAQLDTGRQDLPDRMRQMWRFLVPLVAPEECVLRAVIKL"
     gene            6806..7579
                     /db_xref="GeneID:96295514"
                     /locus_tag="IE233_RS37670"
                     /old_locus_tag="GCM10010326_76940"
     CDS             6806..7579
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (11-180). E-value: 5.8e-11.
                     Score: 34.6. Matches aSDomain:
                     nrpspksdomains_IE233_RS37670_PKS_KR.1"
                     /codon_start=1
                     /db_xref="GeneID:96295514"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010270955.1"
                     /locus_tag="IE233_RS37670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_76940"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_161256562.1"
                     /sec_met_domain="adh_short (E-value: 1.9e-34, bitscore:
                     110.4, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 2.7e-32, bitscore:
                     103.8, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPDISGALRNKTAVITGASSGIGAAAARLFADEGAAVVLMARRKD
                     RLEELAAEIRAGHGKAVAVPGDVCSPEDVARVVARAVEEFGGLDLAFNNAGWGSLGPAL
                     HQLDDSVYDLAMEVNVRGVWNCLRAEIPVMLRQGRGGAVVNTSSVAGHCATGAVAPYIA
                     AKHAVLGLTRAAAAEYGAQGIRVNALMAGSTRTDLLEPYLVDNPELEQAMTRRAIQKRL
                     ADPAEVARVVAWLCSDQASFVTGGAIPVDGGWSAV"
     aSDomain        6839..7345
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS37670_PKS_KR.1"
                     /evalue="5.80E-11"
                     /label="IE233_RS37670_PKS_KR.1"
                     /locus_tag="IE233_RS37670"
                     /protein_end="180"
                     /protein_start="11"
                     /score="34.6"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="TAVITGASSGIGAAAARLFADEGAAVVLMARRKDRLEELAAEIRA
                     GHGKAVAVPGDVCSPEDVARVVARAVEEFGGLDLAFNNAGWGSLGPALHQLDDSVYDLA
                     MEVNVRGVWNCLRAEIPVMLRQGRGGAVVNTSSVAGHCATGAVAPYIAAKHAVLGLTRA
                     AAAEYG"
     CDS_motif       6845..6934
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE233_RS37670_0001"
                     /evalue="1.60E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE233_RS37670"
                     /protein_end="43"
                     /protein_start="13"
                     /score="17.6"
                     /tool="antismash"
                     /translation="VITGASSGIGAAAARLFADEGAAVVLMARR"
     gene            complement(7644..8873)
                     /db_xref="GeneID:96295515"
                     /locus_tag="IE233_RS37675"
                     /old_locus_tag="GCM10010326_76950"
     CDS             complement(7644..8873)
                     /codon_start=1
                     /db_xref="GeneID:96295515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014158290.1"
                     /locus_tag="IE233_RS37675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_76950"
                     /product="transposase"
                     /protein_id="WP_229893118.1"
                     /transl_table=11
                     /translation="MTSVAFGQALSPMQETGLRITTYADQLFTHLPRADQRRWAAAYLL
                     GLLAVSGRKSVRSLAAAVTDSPTASQSLHRFVNSSPWPWQPVRGELTHWVEERLRPKAW
                     TVGTAVVPKRGNQSCGVHRRFVPALGRTINCQVGIGLFLAAGHGQVPVDWRLHLPGEWA
                     DDRLRRRARVPDAARPLPVWAHVLDLADSLASRTSTPLPLVANLGEYKESDRLLQGLRQ
                     RGHDFVVTVPPTLPVLTPAPVPGRGGDPSALTSAQSFLEASGEVPRSVVVSTHSGSTRL
                     TRTVTGLVRLPRGAHAARFYRLFAEYPREGRGAPVIRLTSLVHRPMDELLQLAELQAGT
                     PRALRHLEGNFGLQDFEGRSYPGWHRHVTLVSAACAYSRLAEAQAADSVETVPLNWLQA
                     DSRGTADLLV"
     misc_feature    complement(8781..8783)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            9325..9936
                     /db_xref="GeneID:96295516"
                     /locus_tag="IE233_RS37680"
                     /old_locus_tag="GCM10010326_76960"
     CDS             9325..9936
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96295516"
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014158289.1"
                     /locus_tag="IE233_RS37680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_76960"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_161256539.1"
                     /transl_table=11
                     /translation="MARQERAVRTRQTLVDAAASEFDRSGYHGTSLNRVCRAAGITMGA
                     LTFHFSAKDELADEVQSQGQLITRQALDGLPERSAPALHRAIDLTLELARLLEAEPVVR
                     SAARLSRERPDRDLAWSAAWLTNVQDLFDQAYHDGQLHSAVRPASLTALSLYLMSGIES
                     QARADHESPDAEPELSAVEQLRQIWGVVQFGICGPPGTGC"
ORIGIN
        1 ctcctccgcg gcagtggccg aggtcccgcg ggctcggagc gactcttcac ccgcttggcc
       61 gggagtgcgc cccgtctcgt aggatcgccg catggtggcc tttgtaccgt cgttcgtatg
      121 cttcttcctc ttcggtgtcg gtgtcgtacg cgaccgccgc cgagtgagca acgcggttct
      181 cctgggcctg gccatcgcct ttgcggcgct ggcgctgcta ctgcatgtgg cctccgagca
      241 ggcccagttg ggccgcgacc tggtcatcgt gctgctgacc ctcgcgggcg tgggtgtcgt
      301 cacgctggcg tggttcctca tcgccaacgg gatcaccatg gtccgcaagg agggcaggag
      361 cccagcgaat ctgctgtccc tcggagcggg cctcgtcctg gttgccctgc tcgcactgct
      421 gatcaccgcg ctggtgctgc acacgcacac gcttttggta gtggcggcaa cggcggtgac
      481 gctcgccgga tacgtcgcct tcctgttcct ctgtttcctc gtctacgggg gcctctacgg
      541 gcgtctgcgc atccggcgcc gggtggacta cgtggtggtc ctcggctcag gcctgatcgg
      601 cggcaccacc gtgcccccgc tgctggccgc ccgcctcgac cgggcccgga aggaacacgc
      661 ccggctgtcc cggaaggggc ggcgcacgct tctcctcacc tcgggagggc aggggcccga
      721 cgagaagctg ccggagtctc atgcgatggc cgactacctg gtcgcccagg gcttccccgc
      781 cgacctgatc gaacgtgaag accgctcgac gaccacggac gagaacctga agttcagcaa
      841 ggtcctgatg gagcgggcga acccggacta ccggtgcgtg atcgtgacga acaactatca
      901 cgtcttccgc gccgcgatca ccgcccgccg caccgccgtc cggggccacg tgatcggcgc
      961 cccgacagcc gcgtacttct ggccgagcgc catgatccgc gagttcgtgg ccctgatggt
     1021 cgcctatcgc cgcaccaacg ccacactctt cctgctcgtc atgctgagcg gcctgttcat
     1081 ctggtggctg gggtggccgt cccccggggc cgtcacatag aagtcgaaaa tccgcagtgg
     1141 cacaaccgcc cagtgttgac ggccgagacc aagagccgcc caacagggga accggccagc
     1201 tggtcgtgcc acttggcttg atcaccgtct cgttcggcca ggccctccgc acctgctggt
     1261 accgcttgcg cgtttcatcg ccccagccgc agctcacgtc cagccaggct tggaccgagg
     1321 agcttgactc ggtgtagtcc ttcaccgtga cgacggagac gggcacagag aactggcggt
     1381 cgttgtccat gcaatctcgg tattgcgcag tcggcggacg ccccgtgggg ccggatgggg
     1441 acggttccga actggccgct gtcgagccgt tcggtgacca ggcgccagag ccttgccaga
     1501 ctcctcatgg tgggctcgtc gccggagccg gctcgcgcag gaggttcgcc acgtcgacca
     1561 cagtggctac ggtgtcgagt cgggtgatgc ccggccagtt ccgctacgcc ccggaccagt
     1621 tgggcggtgc cttgatcgcc gaggccgatc cggcggccca gcgcggcggc ggccagcgcg
     1681 ccgcggggcg gcagcatcga gatctcctgc accattcccg aagccgcagc cctcggtttt
     1741 ccccagaccc acctgatgtg gcgacatgtt gccgagcggc tcgccgacag gcatacggtg
     1801 ttctgtcccg acctgcgggg ctacggcgcc agcgacaagc cggcggccac cgaccccgag
     1861 gtgtacgcca agcgcaccat ggccgccgac atcgtcacgc tggcggcgtc gctcggccac
     1921 gagcgctgtg ccctcgtcgg ccacgatcgc ggggcgttgg tcgccttccg cgctgcactg
     1981 gaccacccgg agaccatcac tcacctgggc atcctcgaca tcgtgccgat gctcggcatg
     2041 tggaacgtga tgcggggcgt ctcggcggcg gtcggctgtc acctctacct ggtggcgcag
     2101 ccgccgggcc tgccggaggc gatgatcgca ggcaacgcgg acgcgttctt cgggtcgttc
     2161 ctgggcgctt gggccaatga tccgaccgcc atgcccgcag atgtgcgtgc cgaatacctg
     2221 cgtgcgagcg gcgcggcggt gacgtcggtc gtcgcggact accgggcctc ggcggacatc
     2281 gacgtcacgc atgaccaagc ggacctggac gccgggtcgc agctggccat gcctgtcacc
     2341 gtcatgcagc aggactgggg cgcgcagctc ggcttcgacg ctgccggcat gtcggaatat
     2401 cccgctgtgc cagcaacagc cgtgggttga tctcttgaaa gaaactggaa agtgggggag
     2461 ttgcgcccgg acaggggcgg tgagggccac gggcgtagct ctgctgaggc ggcgggtggt
     2521 ggtgggagtc atcgtccgaa ggctcttctg cgaccgaagg gcctgccccc ggaggacgtt
     2581 cgccgagcag ctcgacggac tcggcagaca ggcccacttc gtgcagtgct ctcagcgtcg
     2641 cggtcccgac aagatcaaat tgttgggcca cgagtagtga tcggaccgag gctgcgtgtc
     2701 ggcgctcggc gatctggcgg tgactcaact cggctttgct gtaagcatgt tgggcgagcc
     2761 ggaacacggt tcaatcatgc ggtcaggtca agccattcgc caatggccag gacacgtgcg
     2821 cgtggtccgg cgtgctcgcg caactccgcc gccgggtcag cgacttcacg gtatcccgtg
     2881 atcttgtcca gctggtccgg ctcgtagtgg atcggcacca cgtaccgagc gtcgaggatc
     2941 tccgccgccg cggccgcctc cctcgggtcc agcgcggcag gaaccgggct cggcggctgc
     3001 aggtgcggtg cgtcgacgac ggggccgttg gcgggcagga atgccgcgtc gaacgggctg
     3061 aaccggcgcg cgatgagcca ccagtagccg tggaacatgg tgtcgccgcc gtggaagacc
     3121 cgctgcccgt ccgcctccac cacccagttc agctgggggt cgcccagtcc gtccacggcc
     3181 ggtacggcgg tgacgcggaa ggggccgatc tcacgggtgg cccaggcgtc gatgaccgtg
     3241 gccgccaatc ggtgccgagt cagctcactt tcggccagca gggtcgccac gttgtccacg
     3301 tcatcaccat ggccgggagc cgggcgcagc accggcgctc cgggtgtcag cacctccgcc
     3361 agggcgggcg cgtccgtgtg gtcccggtgc aggtgggtga ccagtgcggc atcgaccttt
     3421 cccgtcggcg ctgccaggca ttcaccgcgc ttccaccccg tgaacagtgg ggagaggtcc
     3481 cgcacgtagt cgaccaccag tcgcttggcg ccggcctcga tctccagtcc cgcccagccc
     3541 aaccgtcgta ctcgcatctc tcactccttg ctctgggtaa caccgggcaa gttagcgtac
     3601 gactgttctc taagtaaata gcggacgctc gtacgctatg cttgctgcat gtcgccacgc
     3661 cgctcagtag ccgaagcgca ggccacgaag ggtcgaatcc tcagccgcgc cgccgaaata
     3721 gcctccgagg aggggttgga cggcatcacc atcgggcggc tcgccgaagc gctggagatg
     3781 agcaagtccg gagtgcacaa gcatttcggt accaaggagt cgctgcagat ctccacgctg
     3841 gacaaggcat tcgtggactt ctggcaccgg gtggtcgagc ccgctctggg cagcccgcct
     3901 ggcttgcggc ggctgcgggc ggtgtgcgcc aactccgtga ggtacctgga ggcgccgcta
     3961 ctcccggggg gatgcctgat gaccgcagcg ctcaccgagt acgacggacg ccccggccgg
     4021 gtccgggacg cggtggccga ggtgtggtcg cggtggcagg aacaactgcg ggcggacctg
     4081 accgcagcgg tggagagcgc cgaactgccg gccgggttcg acatcgacca ggccctgttc
     4141 gaaatcatcg ccgccgggct ggcactgaat gcggccatgc agctccagca cgaccgttca
     4201 gccgcggacc gggcccgcct ggcgatcgag cgggcgctca gccagccctg acctcaggac
     4261 agatccgcca ctgccggtaa ggccgtcagc acgtcgcgcg caacagaggt ggggcgggtg
     4321 cgaggccgga ggttccggcg gtgacgcagc aggccgaaaa gcgaggtcag aggtcgcgcc
     4381 gcgagccatt caggtcgaga tatccggagg aaatgtgcgc ggggaaggag gcttcgtccg
     4441 cacggatttg accatcgcat ccgcgggcgg acgaggcagg cggcgttctg cccggcgggc
     4501 cgtgggcaac ggtcacgggt gcgggccccg tggggccgcc gccgggcggg agatgagccc
     4561 gcgccgatag ctgtgcgacg gcgcacacag ccagcggggg tctccgcgat ggcgatcggc
     4621 ggaaccgccc ggcggtcagg tcacggcacg ccacaaacta ccgctggaca tcctgaatct
     4681 tgacgaagac ccacccggct tggggcgtcg gtggcgcagt gctgccgtgg aggtggaggt
     4741 ggcgtgctcg gccaccggtg gatcgtctcg taggtgacct tgactgcctg ctccgtcatc
     4801 tgctcctcga catcgcgcag cgacagggcg aaccggtggt gcagtcacac acagtggctg
     4861 atgacctccg ccggatactg ggacccctat gtacgacgga gtctccacga tcaggccctc
     4921 tccagcgtcg gacgacaaga tgatcacgct agcctggctg gacccgctat gtttcggctc
     4981 ctgttcctgt gcgcctgatg tcagttgtac tcggatgcgg tgacgaggat cgagaagact
     5041 tcgctgtctt tctgtcggcc ggtgagccgg gtgctgatga ccccgggtgt ccggcggcgc
     5101 agcggggtgg cttcgagcca gcagggggag tccagttcga cgtaccggtc gaagtgtgcg
     5161 tcgaaattca tcgggacggt gcggcgggcg cccgagaggg cgtgggcggc ctgggctgcc
     5221 gcctccagga agacgaggcc gggtatgtgg tcgtggtcat ggtcgaagaa ggtgcggttg
     5281 gcggtgttga cccgcaactg ccagcggtgg gactcgccga gcggggagag gactgcgtcg
     5341 tccagcgacg tttgctcgcc gcgtacggac ggggctagcg cggggccgag ggggagggcc
     5401 cgcgcggtgg cggccgtggc gtcggcgtgg tccccgcgca gacggttgta gatggccgga
     5461 gggtgggcgg tgtagcggat gcgcgcgatg ccgagcggct ctttgtcgcg ggtggccgtg
     5521 atctgggcag cgaggtgagc ggagccgaag cggcggcggg aaacctcggt atgagtgatc
     5581 gtgaggcgga cgacgcttgg ggtggagcgg gtgcgcagag cggtcgggat cagggacgta
     5641 tcgaaggatt cccacccaag gcggtgatcg agggggatgt cgtaggcggt gtgggacagc
     5701 aaggccagtg cctggcggat cgtctcggcc aggagcaatg ggtgatagag ctcgtggtgt
     5761 gacgtgtaaa acccgtgtgt ctggggccag ttcgcgacga cagtgtgcga gacgccgtca
     5821 ccgagacggc gccaggcggt gaggagtgct tggctggcga ctgtcttgcg gaccagtgcc
     5881 attgggacgc gctgggtgct ttcgatggtc atgatcgagc cagttgtgac agtcattcgg
     5941 agtcccccaa aaacggcgcg agcaagagac agacagcgac tacggacatg ggttcgaatc
     6001 tctgtccagt aacatagaga tgactatttt tattgtcgag ggttttgcga ggactgagaa
     6061 tggtgcagcg tgaaccgaag caggagcgca gtgcgcgtac gcgggaggcc atcctgcgag
     6121 cgggggccga ggtcttcgcc gaagtgggct tcgccggtgc gagcgtgaac aagatcgcga
     6181 ggcgggccaa cctgaccctg ggggcgctgt acttccactt cgagtcgaag gagcagctgg
     6241 cccgcgagat cgtccaggcg cagcccaccc agatcacgct gccggccacc gtgcatggcc
     6301 ttcagggcgc agtggacacc accatcgcat gggcgaccca actcctcaac gacccgatgc
     6361 tgttggcggg cgcccggctg gtgatggacc aggagtattt cgtcgacgcg gagaagggga
     6421 actcccacca gcagtgggtg gaggtgctgc ttccgttcct ctacgaggcc gcccaagcgg
     6481 gcgagctgaa gccggatgtc gatgtggagg cgctggcacg gctggtggtg aacgcggcca
     6541 ccggcgcgca gatgaacgct cagctcgaca cgggacgcca ggacctgccg gaccggatgc
     6601 ggcagatgtg gcgcttcctg gtgccgctcg tcgcgccgga ggagtgcgtg ctccgggctg
     6661 tgataaagct gtgactaccc atcatgttgg ttgtggttgg ggggagttgg cgagcggcct
     6721 ccgctcggca gggtggcgaa aagccgtcac cgtcgcttcg gtctaaccgc tgcgcggccg
     6781 ggtgtggatg gcattcaatc ggctcatgcc agatatttca ggagcgttgc ggaacaagac
     6841 cgccgtgatc accggggcct cgtcaggtat cggggccgcc gcggccagac tcttcgcgga
     6901 tgaaggtgcg gccgtagtcc tgatggcgcg gcgcaaggac aggctggagg agttggccgc
     6961 cgagatacgc gccgggcacg gcaaggcggt ggctgtgccg ggagacgtgt gcagtcctga
     7021 ggacgtggcg cgagtcgtgg cccgggcggt ggaggagttc ggcggcctgg acctagcctt
     7081 caacaatgcg gggtgggggt cactgggccc cgcccttcac caactggacg attctgtcta
     7141 cgatttggcg atggaggtca acgtacgggg agtgtggaac tgtctgcgcg ccgagatccc
     7201 cgtgatgctg cgtcagggcc gcggtggagc cgtcgtcaac acttccagcg ttgccgggca
     7261 ctgtgcgacc ggggcggtag cgccctacat cgcggccaag catgcggtcc tcggccttac
     7321 gcgggccgcc gcggccgaat acggtgctca gggcatccgc gtcaacgcgt tgatggcggg
     7381 ttccacccgt acggaccttc tggaacccta tctggtggac aacccggagc tagagcaggc
     7441 gatgactagg cgcgcgatcc agaagcggct cgccgacccg gccgaagtcg ctcgcgtggt
     7501 cgcctggttg tgcagcgacc aggcctcctt cgtcacgggc ggcgcgattc cggtggacgg
     7561 cggctggagc gccgtctgag gggcaccggt cgggaggccc ccgcgtgggc gccggcagca
     7621 ggcgcgcacg cggtgcaccc gattcagacc agaaggtccg ccgtgccgcg cgagtcggcc
     7681 tggagccagt tgagcggcac cgtctcgacg gaatcggcgg cctgggcctc ggcgagtcgg
     7741 ctgtaggcgc atgccgccga caccagggtc acgtggcggt gccagcctgg gtaggacctg
     7801 ccctcgaagt cctgcaggcc gaagttgccc tccaagtgcc gcagtgcccg cggcgtcccg
     7861 gcctgcaact cggcgagctg tagcagttcg tccatgggcc ggtgcaccag gctggtgagc
     7921 cggatgacgg gggcgccccg gccctcgcgc ggatactccg cgaacagccg atagaagcgc
     7981 gctgcatggg ccccgcgcgg cagccgcacc aggccggtca cggtccgcgt cagccgggtc
     8041 gagccgctgt gcgtgctcac caccacggag cgtgggactt ctccgctcgc ctccaggaag
     8101 ctctgcgcgc tggtcagcgc cgaagggtcg ccgccccgtc ctggcaccgg agccggcgtg
     8161 agtaccggca gggtgggggg aaccgtcacg acgaagtcgt ggccgcgctg tctcaggccc
     8221 tggaggagcc ggtcggactc cttgtactca ccgaggttcg ccaccagcgg cagcggtgtc
     8281 gaggtacggg aggccaagga gtcggcgagg tcgagcacat gggcccagac cggcagcggg
     8341 cgggccgcgt cgggaacgcg ggcgcgccgg cgcagacggt cgtccgccca ttcgccgggc
     8401 aggtgcagcc gccagtcgac cgggacctgg ccgtgcccgg cggcgaggaa cagaccaatg
     8461 ccgacctggc agttgatggt ccggcccagt gccgggacga accggcggtg cacgccgcag
     8521 gactggttgc cccgcttggg taccacggcg gtccccaccg tccaggcctt cggccgcagc
     8581 cgctcttcga cccagtgggt cagttccccc cggaccggct gccagggcca cgggctggag
     8641 ttgacgaagc ggtgcaatga ctgcgatgcg gtgggggagt cggtgacggc ggcggccagg
     8701 ctgcgtacgg acttcctgcc cgagacggcc agcaggccca gcaggtaggc ggcggcccaa
     8761 cggcgttgat ccgcccgcgg taagtgcgtg aagagctgat cggcgtaggt ggtgatgcgt
     8821 agccctgtct cctgcatggg cgagagggct tggccgaacg cgacagaggt catgtggggc
     8881 tccaggctgc ggctcgcgga ctgactaccc aaagaataat agatgtcgct attttttatt
     8941 gcagtcgaat cgcccaacct gcaacgagag gtgccgtcct gcaccgccac cgatccgctc
     9001 ggtacgaacc agccaacccg caggaccccg acttggtctc gctgacctgc ggagtccaca
     9061 cttgcggcca cgatcactgg acagccaagt cggcccacgg tcgcggcccg cacaacgggt
     9121 cgcccggcgc ggcccgctcc catagcggtc cgccgcagcc gtaccacccc acgacggcac
     9181 cggcagggcg cgagggagtg aggccgcgcc ccgcgcagtg cgcgcacgct gcatcgcggc
     9241 ggacgcggcc cgcgaccgtc ctccccgggg cgacggaagc ggcgacgtct tgctgatcgc
     9301 caacgagcgc acccggagag acgcatggcc agacaggaac gtgcagtacg cacccggcag
     9361 accctggtgg acgccgccgc ttcggagttc gaccgcagtg gttaccacgg cacctccctg
     9421 aaccgggtct gcagagccgc cggcatcacc atgggggccc tgacgttcca cttctcggcc
     9481 aaggacgagc tcgccgacga ggtgcagtcg caggggcagc tcatcacccg ccaggccctc
     9541 gacggcctgc cggagaggtc ggcccccgcg ctgcaccggg cgatcgacct cacgctcgaa
     9601 ctcgcgcgcc tcctggaggc ggaacccgtg gtgcgctcgg ctgcccggct cagccgcgaa
     9661 cgccccgacc gcgacctggc ctggtcggcc gcatggctca ccaacgtgca ggacctgttc
     9721 gaccaggcct accacgacgg tcagctgcac tccgccgtgc gtcccgcctc gctgaccgct
     9781 ctgtcgctct acctcatgag cggcatcgag tcccaggcgc gcgcggacca cgaaagtccg
     9841 gacgccgaac cggagttgag cgccgtcgaa caactcaggc agatctgggg cgtcgtccag
     9901 ttcggtatct gcgggcctcc ggggacgggg tgctgagcca cccgaagcac ccgtcgttcg
     9961 caccacagta cgaaactcgc tgacgatttt cgcgagtcga cattgccgca ccggcctcct
    10021 gaacggatcg tagaactcgt aaccgacaca cctacgaggg ggaccgtggt ggagacggaa
    10081 ttgactgtcg ggatgcgcgc aacggcggac tgccgccgtg ttgccgcgct cttcatgcgc
    10141 atgtcgctgt cagcatccgc atgaagagcc cgggagaccg cctgccacca atggctctct
    10201 ccggctgaac cccttttccc ggtggttggt tgaggctgcg cacgccctcg tccaccacgg
    10261 gccgggacgg tggcggggcg acggttggaa agcttcccct accacgctcc tccgtgctcc
    10321 cgccccaagg caggcacgtc ggtctgacca gcccggattt ccggctgcct cttttcgggt
    10381 ggcctacatc gtcacgcctt gaaaagactc ctgtggaggg ttcgatggcg caagctgagc
    10441 gaatggacgc ggtggtgatc ggtggaggca tcgccggatc cgccctggcg gcggcactgg
    10501 cgggcgacgg ctaccaagtg ttgctcctgg agcggcagac cgtctaccgg gacaaggtgc
    10561 gcggcgaggt catcaactgc tggggagtgg tcgaactgct cgaactcggc ctggaggaag
    10621 ccatcctcaa agccggcggc agctatgtgg accgcttcat cggctacgac gagatcgtcg
    10681 acccagacgt cgcccaggcc aacgcgatcc cgctcgacga gatgctcccc ggcatacgcg
    10741 gcgtgctcga cgtgggccac cccgatgcct gcgaggctct tgccaccgcc gccgccgagg
    10801 ccggagccac cgtcgtccgc ggcatcggcg acgtcaccgt caccggtggc gccaacccca
    10861 gcgtgcgcta cgagtacaac gacatcgagt acgacgtgcc ctgccggatc gtcatcgggg
    10921 ccgacgggcg gacctc
//
