LOCUS       NZ_BMUU01000006        11938 bp    DNA     linear   CON 09-NOV-2024
DEFINITION  Streptomyces xanthochromogenes strain JCM 4594 sequence06, whole
            genome shotgun sequence.
ACCESSION   NZ_BMUU01000006
VERSION     NZ_BMUU01000006.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces xanthochromogenes
  ORGANISM  Streptomyces xanthochromogenes
            Bacteria; Bacillati; Actinomycetota; Actinomycetes;
            Kitasatosporales; Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 557778)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650355.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:28:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,827
            CDSs (total)                      :: 7,752
            Genes (coding)                    :: 7,642
            CDSs (with protein)               :: 7,642
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 110
            CDSs (without protein)            :: 110
            Pseudo Genes (ambiguous residues) :: 0 of 110
            Pseudo Genes (frameshifted)       :: 24 of 110
            Pseudo Genes (incomplete)         :: 94 of 110
            Pseudo Genes (internal stop)      :: 7 of 110
            Pseudo Genes (multiple problems)  :: 12 of 110
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 16:05:05
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 391872
            Orig. end                         :: 403810
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUU01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11938
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11938
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11938
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6938](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6938)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            396..803
                     /db_xref="GeneID:96292032"
                     /locus_tag="IE233_RS20030"
                     /old_locus_tag="GCM10010326_40940"
     CDS             396..803
                     /EC_number="3.5.-.-"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96292032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003949595.1"
                     /locus_tag="IE233_RS20030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_40940"
                     /product="RidA family protein"
                     /protein_id="WP_190027897.1"
                     /transl_table=11
                     /translation="MTITLVNPEGLPEVGVYHQVSVASGSKLVFLAGQVSVDADATTLG
                     EGDLAAQTERCYLNVATALAGAGATFADVAKLTVYVVDWAPEKMPLLLDGIARASAALG
                     TAIAPPGTLLGVAALASPAHLVEVEAVAVLD"
     gene            complement(829..2892)
                     /db_xref="GeneID:96292033"
                     /locus_tag="IE233_RS20035"
                     /old_locus_tag="GCM10010326_40950"
     CDS             complement(829..2892)
                     /codon_start=1
                     /db_xref="GeneID:96292033"
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962841.1"
                     /locus_tag="IE233_RS20035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_40950"
                     /product="regulator"
                     /protein_id="WP_190027898.1"
                     /transl_table=11
                     /translation="MAGKIPDETSSFVGRREELRRVERALSVHRLVTLTGTGGVGKTRV
                     ALRAAARGGPGHRDGVIWAELWPLMGSDLLIATVADAAGLADHTPRLAADALCAWLAER
                     EALLVLDSCEHLAADCRRLIGQLLCSAPGLTVLATSRQPLEVDGEHVLAIGPLPVDGTR
                     GARAPSDAARLLLSRAAEAAPDVDVDSPSERAAADAICRRLEGIPLALELASGQLRQRR
                     LTDLAGALGSPLTMLSGEPSARPPRHASLRTTIGWSHELCTPAERLLWARLAVLRGPFD
                     ARTAQLVCSGGPLSEDGVADALSGLVVKCVVQRDGDRHRMLDSLKEYGRMWLEELGERE
                     QLADRHAAYFHEVVRQADERWLGPEQAREYGRIAQAHPDLCAALDHLMNDPDGAAVDLA
                     GRVAFFWSCCGHLHEARHYLERALLEHREPGPVLTRALWALGIVRLLQGEPEPAAQLAA
                     HCARAAEEDEDPEAALGAAYLLSLTYLMTGRPLVAQSLAESALAEAAEDDFVSPSRLRC
                     RLITVFTRTALGVYDEAEGLARRLLAACEQRDEVWTRSYVEYQLSLIALFRDRPAEAAE
                     HARAMLRGKRRLGDRFGIALGVDLLAAAAAEQGEGEQAARLSGTGQTYWRSVGHPQRGT
                     PELAAVRERTDLRARTAIGDHAYRVARQRGASDTRATLALALGPAEPPAPPE"
     gene            3093..4202
                     /db_xref="GeneID:96292034"
                     /locus_tag="IE233_RS20040"
                     /old_locus_tag="GCM10010326_40960"
     CDS             3093..4202
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96292034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019525618.1"
                     /locus_tag="IE233_RS20040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_40960"
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="WP_190027899.1"
                     /transl_table=11
                     /translation="MADTSQTPSSYWSRLRGRTADPAARETAGATPDVEPAGHTASPAG
                     SDDEPSAPTDLPARSWLAVLRRTGREFLDDDLPDKAAALTYYGVLSLFPALLVLVSTLG
                     VMGEKATRGILDNLGHLTPGPVHDLLDSAIRQLQGNGGTSSVVAVVSLIAALWSASSYV
                     AAFIRAANAVYDLPEGRPVWKLTPLRLALTVTLMVVLAVSAVIVVFTGPLAQRAGDAIG
                     AGDAAVATWNIAKWPVLLLLMVFMVALLFWRAPNVRGPGLRWLSPGSLLAVLLWLAASG
                     GFALYVANFGSYNKTYGTLAGLVIFLIWVWLSNLAILLGLELDAELARQRAISGGHPEG
                     EEPYVEPRDTRKWPARLRRRVRRRRNTSK"
     gene            complement(4286..4963)
                     /db_xref="GeneID:96292035"
                     /locus_tag="IE233_RS20045"
                     /old_locus_tag="GCM10010326_40970"
     CDS             complement(4286..4963)
                     /codon_start=1
                     /db_xref="GeneID:96292035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003958122.1"
                     /locus_tag="IE233_RS20045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_40970"
                     /product="DUF5825 family protein"
                     /protein_id="WP_190027900.1"
                     /transl_table=11
                     /translation="MSTALTTTTEALGVAVWRDYDPEACTLPGMYLGEMELTGPPAEES
                     DRLWKLGARRVRLPEPVDLADLAAPGAAERAVRALSLVRDLTARAVLVEWKLRLDPAAP
                     DDTWRTLSHLQPPQRLEGPADATADLRRWRDGHYLCKCLWRQGPGFVQIRDRRWGDLRR
                     FTAEEPEYHRTIEALAYGAPAASVPAAVLADFLGERLVLAVGELAWWMPYRVNRWIQEA
                     MAI"
     gene            complement(5001..6938)
                     /db_xref="GeneID:96292036"
                     /locus_tag="IE233_RS20050"
                     /old_locus_tag="GCM10010326_40980"
     CDS             complement(5001..6938)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96292036"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03975"
                     /gene_functions="other (smcogs) SMCOG1277: Radical SAM
                     domain protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019357532.1"
                     /locus_tag="IE233_RS20050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_40980"
                     /product="RiPP maturation radical SAM C-methyltransferase"
                     /protein_id="WP_161244571.1"
                     /sec_met_domain="TIGR03975 (E-value: 2.9e-186, bitscore:
                     615.4, seeds: 14, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLLVNMPWSPIDLPSLALGILKRSVDERVPGASAEVLHANLEF
                     TDWITERTEFTSDDYEYYALSSYFMGCGDWVFSSALYDDPAWRDEEFAAAMKGKLRKSR
                     LRMSQELHRQVPEFVEMIAQRIVEYAPDVVGFTSTFQQNTAALAAAKHVKRLAPHIVTV
                     MGGANCDGEQGEAVHRNFPFVDHVIRGEGESAFPQLLTAIAEGAGDEVLSKVPGLCHRD
                     PEGRSVVNAMATSPLPPATILAPDYSGYFERLATSVARNWVEPKLVVEGARGCWWGEKH
                     HCTFCGLNGSFMQFRSKSPDTFFEEIMELARRHRVLDMYLVDNILDMGYLTTVLPRIME
                     SGYDLRMHIEIKANMRRSQLRTLAEAGLIYVQPGIESLNNRVLDLMDKGVSGCQNVRML
                     RDGAETGLSVAWNYLHGFPGETAEDYDPVIAQIPALEHLNPPVDLSARIAIERFSPYFN
                     RPELGFDGLRPEEHYRFTYDLPESELYDLAYVFEAPQRGITEPTVTALNDALDAWKKHH
                     ADARLTHTDLGDRIVLVSRRRTFAWRAMEVTDPFELAVFRLLDQPHAVTALTRKAAARA
                     KGAERTEHEVQQLLDSWLALGLVFTDGGQYVHIAPSAVNEDLLRLDFMRHAQAALDADE
                     QPARPEPVSV"
     misc_feature    complement(7095..7115)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.21"
                     /tool="antismash"
     gene            complement(7148..9025)
                     /db_xref="GeneID:96292037"
                     /locus_tag="IE233_RS20055"
                     /old_locus_tag="GCM10010326_41000"
     CDS             complement(7148..9025)
                     /NRPS_PKS="Domain: Abhydrolase_1 (353-512). E-value:
                     3.8e-10. Score: 31.7. Matches aSDomain:
                     nrpspksdomains_IE233_RS20055_Abhydrolase_1.1"
                     /codon_start=1
                     /db_xref="GeneID:96292037"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129145.1"
                     /locus_tag="IE233_RS20055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_41000"
                     /product="alpha/beta fold hydrolase"
                     /protein_id="WP_190027901.1"
                     /sec_met_domain="PF00561 (E-value: 2.4e-08, bitscore: 28.9,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Peptidase_S9 (E-value: 9.9e-37, bitscore:
                     121.5, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSRSYQVYRAALPEVCAAQPAKMVFTADADGRCEVFTWDADTRRA
                     RQVTDRPHGTLHGAIDRDARVWWFDEDDHGSGRWRFQPFEGGPDLPGLLGVPEGEPRGL
                     AVSDGGRVAMGIGDARSTTVYLGPRGGAAREVTRVDGHATLSGITPDGRLLALGGAAGS
                     ARAVTVLADDGTQCAVLAGDGGGGRLWSLGFAPTARHAELLLVRESNNRYLLASWTPGT
                     GLVTHSWCAFDTEITARWHPEGHSLLVRQDRHGRSTLHRVHLAERTVEPVPTPRGTLLD
                     AAPRAGSDVHVLWTDTATPPRMVSTAGTELPPTHGIPERVPGEHRDLWTPGPDGPVHSL
                     LSLPEGRTGAVPGVFLVHGGPADHDRDAYDGVVHSLVASGFAVVRVNYRGSTGYGPRWR
                     RAFGEGVGLTQVADVVAVRADLVARGVVRADAAGLWGTSWGGYLVLLALGTHPDLWQAG
                     VAVKPVADCATAYRTTTPALRALDERLFGGTPDEVPERYERSSPRHYVAAVRAPLLVAA
                     ATRDAKCPPGQVRDYLTALQLVGARHESMWLDTGHDGYEADDHVAVLRRAVVFLDRELR
                     QGQARTTTPKTPRSDRSGDSGRERSPAPAGTGGTHTERSSGHAEGHHPQ"
     aSDomain        complement(7490..7966)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE233_RS20055_Abhydrolase_1.1"
                     /evalue="3.80E-10"
                     /label="IE233_RS20055_Abhydrolase_1.1"
                     /locus_tag="IE233_RS20055"
                     /protein_end="512"
                     /protein_start="353"
                     /score="31.7"
                     /tool="antismash"
                     /translation="VFLVHGGPADHDRDAYDGVVHSLVASGFAVVRVNYRGSTGYGPRW
                     RRAFGEGVGLTQVADVVAVRADLVARGVVRADAAGLWGTSWGGYLVLLALGTHPDLWQA
                     GVAVKPVADCATAYRTTTPALRALDERLFGGTPDEVPERYERSSPRHYVAAVRAP"
     misc_feature    complement(8414..8416)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(9022..10335)
                     /db_xref="GeneID:96292038"
                     /locus_tag="IE233_RS20060"
                     /old_locus_tag="GCM10010326_41010"
     CDS             complement(9022..10335)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /db_xref="GeneID:96292038"
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569708.1"
                     /locus_tag="IE233_RS20060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_41010"
                     /product="MFS transporter"
                     /protein_id="WP_190027902.1"
                     /transl_table=11
                     /translation="MTTGTTAAERRAARRPVPRDVRLFWWASASDALGTQASGVVLPLL
                     LLGLGHSAATVGLVAGVSTAAGLLLAPLAAVPADRGSRKAVMFWSATVSALAMAGVTAA
                     VATGEPPLALVLGAVLVERLSTAVYEAAAAGTVAMIAPAADCPRVVAGLAAGDQGALIL
                     GPALGGALYQLARALPFLADAVSYAVAAGCVRALRSDLRPVRDPAPGTAANGSARVAEA
                     AAGPTSTPVPDRGLRSLRSELGAGLALVRTSPLLRLVVTWTATVNGVIVALYYGAVFTL
                     QKGGHGGAPMGLVLALSGAAGLVGSLAAPRVVGRIGAARLLVTVSWLLVPLAAGLATAD
                     GPWTYGALFGVFCLLMPLVAVVLQSRALQETPPSLQARTGAVLASAVGGSAALAPVVAG
                     VFVTRVGTAAPAVICAGLLTALAWRTTVVRSRLTGATA"
     misc_feature    10329..10343
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.18"
                     /tool="antismash"
     misc_feature    10406..10420
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 18.19"
                     /tool="antismash"
     gene            complement(11096..11644)
                     /db_xref="GeneID:96292039"
                     /locus_tag="IE233_RS20065"
                     /old_locus_tag="GCM10010326_41030"
     CDS             complement(11096..11644)
                     /codon_start=1
                     /db_xref="GeneID:96292039"
                     /inference="COORDINATES: protein motif:HMM:NF025013.5"
                     /locus_tag="IE233_RS20065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010326_41030"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_190027903.1"
                     /transl_table=11
                     /translation="MRITHIATTAAAAALTLTGTTAAFGAASPSATADAVFLQAIHQAN
                     LAEIAAGKDAATHASSSCVKQVSAIIVRDHTKLDAKGAALAKTKNITLPKESTAAQQKS
                     LSALKPMNGTAAYDTAWLKVQSSGHQQALALIDKELKSGNDAQIQAAAKEARPIVAAHL
                     KMVEGGECHAMASSSPSPQ"
ORIGIN
        1 gcaccgtggg gtgcacatcg cgttcgacca ggccgttgcg ctccagcatg cgcaggttct
       61 gggtgagcat cttgtggctg atgccctgga tctcgttgcg cagcgcaccg aagcgcaggg
      121 tgccgtcgcc gagcgcctcg atgatcagga acgcccactt gttggctatg tcggtgaaga
      181 tctcccgggc cagcgagtcc gcgcggctca ggtccgcgtc ctctgccgtg cccctgagct
      241 gcttggtcac catcgggttc cccagtcact ggaaagtgcg ttcttccatg tcagcggcca
      301 ctctcctacg gttcccgagt aactacaaga gaccgagggc gagttgggcc ccgcggcagc
      361 gggcccgtgc gctctcgcac acggaagcgg gcggcatgac catcaccctt gtgaaccccg
      421 aaggactgcc cgaggtcggc gtctaccacc aggtgtcggt cgcgagcgga tcgaagctcg
      481 tcttcctcgc cgggcaggtg tccgtcgacg ccgacgcgac caccctcggc gagggcgatc
      541 tcgccgcgca gaccgagcgc tgctacctca atgtcgccac cgcgctggcg ggggcgggcg
      601 cgaccttcgc cgacgtggcg aagctgaccg tgtacgtcgt cgactgggcc ccggagaaga
      661 tgccgctgct cctcgacgga atcgcccgtg cgtccgccgc gctcggcacg gccatcgccc
      721 cgccgggcac gctcctgggc gtcgcggccc tggcctcgcc cgcccatctg gtcgaggtcg
      781 aagcggtggc ggtcctcgac tgaaccggct cggccgggcc gagccggttc actcgggcgg
      841 ggcggggggc tcggccgggc cgagggccag ggcgagggtg gcgcgggtgt cggacgcccc
      901 gcgctggcgc gccacccggt aggcgtggtc accgatcgcg gtacgggccc gcagatcggt
      961 gcgttcacgc accgcggcca gttccggagt gccgcgctgc ggatgcccga cactgcgcca
     1021 gtacgtctgc ccggtgccgg agagccgggc ggcctgttcg ccctcgccct gctcggcggc
     1081 ggccgccgcc agcagatcga cgcccagggc gatgccgaac cggtcgccga gccggcgctt
     1141 gccccgcagc atcgcccgtg cgtgctcagc ggcctccgcg ggccggtccc ggaacagggc
     1201 gatcaaggac agctggtact ccacgtagga gcgcgtccag acctcgtcac gctgctcgca
     1261 ggcggcgagg aggcgccggg cgaggccctc cgcctcgtcg tagacgccca gcgcggtccg
     1321 cgtgaacacg gtgatcaggc ggcaccgcag gcgcgagggg gagacgaagt cgtcctcggc
     1381 ggcctcggcg agggcgcttt cggcgaggct ctgggcgacc aggggcctac cggtcatcag
     1441 gtaggtgagg ctcagcaagt aggcggcccc cagtgccgct tcggggtcct cgtcctcctc
     1501 cgccgccctc gcgcagtggg cggccagctg cgcggccggc tccggctccc cctgcaacag
     1561 ccgcacgatg ccgagcgccc acagcgcccg ggtcagcacc ggcccgggtt cccggtgttc
     1621 gagcagcgcg cgctccaagt agtggcgggc ctcgtgcaga tggccgcagc agctccagaa
     1681 gaaggcgacg cgcccggcca ggtcgaccgc cgcaccgtcg gggtcgttca tcaggtggtc
     1741 gagcgcggca cacaggtcgg ggtgcgcctg cgcgatcctc ccgtactccc gggcctgttc
     1801 cgggccgagc cagcgctcgt cggcttgccg gacgacttcg tggaagtacg cggcgtgccg
     1861 gtccgccagc tgttcgcgct cgccgagctc ctccagccac atgcgcccgt actccttgag
     1921 ggagtcgagc atgcgatggc gatcgccgtc gcgctggacg acgcacttga cgaccagccc
     1981 tgacagagcg tcagccacgc cgtcttcgct caacgggcca ccggaacaga ccagttgggc
     2041 cgtgcgtgcg tcgaaggggc cacgcagtac ggcgagccgc gcccacagca gccgttcggc
     2101 gggggtgcac agttcgtggc tccagccgat cgtggtgcgc aggctcgcgt gccggggcgg
     2161 ccgggcgctc ggctcgccgc tcagcatcgt gaggggcgag ccgagcgccc cggcgagatc
     2221 cgtcagacgg cgctgccgca actggccgct cgccagctca agggcgagcg gaatgccttc
     2281 caggcgacgg cagatcgcgt cggcggcggc ccgctcgctc ggggagtcca cgtccacatc
     2341 gggtgcggct tcggcggccc gggagagcag cagccgcgcc gcgtcgctgg gtgctcgtgc
     2401 cccgcgcgtt ccgtcgaccg gcagcgggcc gatggcaagg acgtgttccc cgtcgacctc
     2461 caggggctgt cggctcgtgg ccaggaccgt caagcccggt gcggaacaca ggagttgacc
     2521 gatgagccga cggcagtcgg ccgcgaggtg ttcgcacgag tcgaggacga ggagcgcctc
     2581 gcgctcggcc agccacgcgc acagcgcgtc ggcggccagc cgtggggtgt ggtcggcgag
     2641 accggccgcg tcggcgaccg tcgcgatcag caggtcgctg cccatgaggg gccacagctc
     2701 ggcccagatg accccgtcgc gatggccggg gcccccgcgc gcggcggccc gcagggcgac
     2761 ccgggtcttt ccgaccccgc cggttccggt caacgtcacc agccggtgga cggagagggc
     2821 ccgctcgacg cgtcgcagct cctcccgccg tcccacgaag ctgctcgtct cgtcaggaat
     2881 cttgcccgcc atggcgcaca gtgtggccca cgagcgcgac tcgggtgcgg cgttcggccc
     2941 gggaccaccc cacggtgtgc ggtgcccgct gccgagcgtc acaccatcgg ggggactcgg
     3001 ccggggcacg tgtcgccggc tccccggtca ccgggacgga gtccccgcct gcgcggacgc
     3061 gtgagcggcg acttcgaggg gacccgagag gcatggcaga tacctcgcag accccgagtt
     3121 cgtactggag ccgtctccgc ggccggacgg ccgaccccgc ggcccgggaa acggcgggtg
     3181 cgacaccgga cgtggaaccg gccggccaca ccgcctcccc ggccgggtcg gacgacgagc
     3241 ccagtgcgcc caccgacctc ccggcccgct cctggcttgc ggttcttcgg cgcaccggcc
     3301 gggagttcct cgacgacgac ctgcccgaca aggcggccgc cctcacctac tacggggtgc
     3361 tgtcgctgtt ccccgcgctg ctcgtgctcg tttcgacgct cggcgtgatg ggcgagaagg
     3421 ccacgcgcgg aatcctggac aacctgggcc acctcacgcc cggccccgtc cacgatctgc
     3481 tcgacagcgc gatccggcag ctgcagggca acggcggtac gagcagcgtc gtcgcggtgg
     3541 tcagcctgat cgcggcactg tggtcggcgt ccagttatgt cgccgcgttc atccgtgccg
     3601 ccaacgccgt ctacgacctg cccgaaggcc ggccggtgtg gaagctgacg ccactgcggc
     3661 tcgccctgac ggtcacgctc atggtggtgc tcgcggtcag cgcggtgatc gtggtcttca
     3721 cgggcccgct ggcccagcgc gccggggacg cgatcggggc gggcgacgcg gcggtcgcga
     3781 cctggaacat cgcgaagtgg ccggtgctcc tgctcctgat ggtcttcatg gtggccctgc
     3841 tcttctggcg cgcccccaac gtccgtggcc cgggcctgcg ttggctgagc ccgggcagcc
     3901 tgctcgccgt cctgctgtgg ctggccgcat cgggcggctt cgcgctgtac gtggcgaact
     3961 tcggctcgta caacaagacg tacggaacgc tcgccggact ggtgatcttc ctgatctggg
     4021 tgtggctctc gaacctggcg atcctgctcg gccttgagct ggacgccgag ctggcccgcc
     4081 agcgcgcgat cagcggcggt caccccgagg gcgaggagcc gtacgtggag ccccgcgaca
     4141 cccgcaagtg gcccgcgcgg ctgcgccgtc gggtgcggcg gcgtcgcaac acctccaagt
     4201 gaagtgcccc tccccgcaag gtgattgagc ctcacacgcc ggagcggccg ccgcgccgtg
     4261 cgcgcggcgg cccgtgcccg gccggtcaga tcgccatggc ctcctggatc cagcggttca
     4321 cccggtacgg catccaccag gcgagctccc ccaccgccag gacgaggcgc tcgccgagga
     4381 agtcggccag cacggcggcg ggcacggatg cggccggggc tccgtaggcc agggcttcga
     4441 tggtccggtg gtactccggc tcctcggccg tgaagcggcg caggtcgccc cagcgccggt
     4501 cgcggatctg gacgaagccc gggccctggc gccacaggca cttgcagagg tagtggccgt
     4561 cgcgccaccg gcgcaggtcc gctgtcgcgt ccgcgggccc ctcgaggcgc tggggcggct
     4621 ggaggtggct gagggtgcgc caggtgtcgt cgggcgccgc cgggtcgagg cggagcttcc
     4681 attcgaccag gacggcacgc gccgtcaggt cgcggaccag gctcagggcg cgcacggcgc
     4741 gttcggccgc gcccggcgcc gcgaggtcgg cgaggtcgac gggctcgggc agccggacgc
     4801 gccgggcgcc cagtttccac agccggtcgc tctcctcggc cggggggccg gtcagttcca
     4861 tctcgcccag gtacatcccc ggcagggtgc aggcctcggg gtcgtagtcg cgccagacgg
     4921 cgaccccgag ggcttcggtc gtggtggtga gggcggtgct catcggtcgt ctcctgcttc
     4981 tgtgtcggac acgcgcgggc tcagacgctc acgggctcgg gccgcgcggg ctgctcgtcg
     5041 gcgtcgaggg cggcctgggc atggcgcatg aagtcgaggc gcagcaggtc ctcgttgacg
     5101 gccgagggcg cgatgtggac gtactggccg ccgtccgtga agaccaggcc caacgcgagc
     5161 caggagtcga gcagttgctg cacctcgtgc tccgtgcgct cggcgccctt ggcgcgggcc
     5221 gccgccttgc gggtcagcgc ggtgaccgcg tgcggctggt cgaggaggcg gaagaccgcc
     5281 agctcgaacg ggtcggtcac ttccatcgcg cgccaggcga aggtgcgccg cctgctcacc
     5341 aggacgatgc ggtcgccgag gtcggtgtgg gtgagccgag cgtcggcgtg gtgcttcttc
     5401 caggcgtcga gcgcgtcgtt gagcgcggtg accgtgggct cggtgatgcc gcgctggggg
     5461 gcctcgaaga cgtacgcgag gtcgtagagc tcggactcgg gcaggtcgta ggtgaagcgg
     5521 tagtgctcct cgggacgcag gccgtcgaag ccgagttcgg ggcggttgaa gtaggggctg
     5581 aagcgctcga tggcgatccg cgccgagagg tcgaccggag ggttgaggtg ttcgagtgcg
     5641 gggatctggg cgatgacggg gtcgtagtcc tcggcggtct cgcccgggaa gccgtgcaga
     5701 tagttccagg cgacggagag gccggtctcg gcgccgtcgc ggagcatgcg cacgttctgg
     5761 cagccgctca cgcccttgtc catcaggtcg agcacccggt tgttgaggct ctcgatgccg
     5821 ggctgtacgt agatgagtcc ggcttcggcg agcgtacgca gctggctgcg ccgcatgttg
     5881 gccttgatct cgatgtgcat ccgcaggtcg tagccgctct ccatgatgcg gggcagcacc
     5941 gtggtgaggt atcccatgtc gaggatgttg tcgacgaggt acatgtcgag cacccggtgg
     6001 cggcgggcga gttccatgat ctcttcgaag aacgtgtccg ggctcttgga gcggaactgc
     6061 atgaacgagc cgttgagtcc gcagaacgtg cagtggtgct tctcgcccca ccagcagccg
     6121 cgcgcgccct cgacgacgag cttgggctcg acccagttgc gggccacgga ggtggcgagg
     6181 cgctcgaagt agccgctgta gtcgggggcc aggatggtgg ccggcggcag cgggctcgtg
     6241 gccatcgcgt tgacgacgct ccggccctcg ggatcacggt ggcacaggcc cggcaccttg
     6301 gagagcacct cgtcgccggc accttcggcg atcgcggtca gcagctgcgg gaaggcggac
     6361 tcgccctcgc cgcggatcac gtggtccacg aacgggaagt tgcggtggac ggcctcgccc
     6421 tgctcgccgt cgcagttggc gccgcccatc acggtcacga tgtgcggggc gagccgcttg
     6481 acgtgcttgg ctgcggcgag ggccgcggtg ttctgctgga aggtggaggt gaacccgacg
     6541 acgtcgggcg cgtactcgac gatgcgctgg gcgatcatct ccacgaactc cggtacctga
     6601 cggtggagtt cctgcgacat ccgcagtcgc gacttgcgga gtttgccctt catcgcggcc
     6661 gcgaactcct cgtcccgcca ggcggggtcg tcgtagagcg cggaggagaa cacccagtcg
     6721 ccgcagccca tgaagtagga ggagagcgcg tagtactcgt agtcgtcgga ggtgaattcg
     6781 gtgcgctcgg tgatccagtc ggtgaattcg aggttggcgt gcaggacttc ggcactggcg
     6841 ccgggcacgc gctcgtccac gctgcgcttg aggattccca gggccagcga gggaaggtcg
     6901 atgggcgacc agggcatatt gaccagcaga acacgcatgt cggctcctcg ggatgtgcgg
     6961 agacgtggag tgaacgaggc ccggggggcc ggagttgggc ggtagtgccc ggtcggtccc
     7021 gcggctcacg gccgcgggac cgaccggcga ggatcagtcc tcttcgccgt cctcggcgtt
     7081 gcggaacgga accttgatgg tcacgccgac gctcggcttg cggttttcct cgtcacccgc
     7141 gagcgggtca ttgtggatga tgtccttctg catggccgga actcctctct gtgtgggtac
     7201 cgcctgttcc ggcgggcgct ggggagcgct cccgtccgga atccccgctc cggtcggagc
     7261 ggggcgtctt gggggtggtg gtacgggcct gcccctgccg cagttcgcgg tcgaggaaga
     7321 ccacggcgcg gcgcagcacg gccacatggt cgtcggcctc gtagccgtcg tggccggtgt
     7381 cgagccacat cgactcgtgg cgtgcgccga cgagttgaag ggcggtgagg tagtcgcgca
     7441 cctggccggg cgggcatttg gcgtcccggg tcgcggcggc gaccagcagg ggtgcgcgga
     7501 cggccgcgac gtagtggcgg ggcgagctgc gctcgtagcg ctcgggcacc tcgtcggggg
     7561 tgccgccgaa aaggcgctcg tccagggcgc gcagcgccgg ggtggtggtg cggtaggcgg
     7621 tcgcgcagtc ggcgacgggc ttgacggcga cgcccgcctg ccacagatcg gggtgtgtac
     7681 cgagggcgag cagcaccaga tagccacccc aggaggtgcc ccacagcccg gccgcgtccg
     7741 cgcggaccac gccgcgggcc acgaggtcgg cgcggacggc gacgacgtcg gcgacctggg
     7801 tgagtccgac gccctcgccg aacgcccgcc gccagcgggg tccgtagccg gtggagccgc
     7861 ggtagttgac gcgtacgacg gcgaatccgg aggcgacgag ggagtgcacg acgccgtcgt
     7921 aggcgtcccg gtcgtggtcg gcgggaccgc cgtgcacgag gaagacgccg ggaaccgcgc
     7981 ccgtccgtcc ctcgggcagg ctgagcagcg agtgcacggg cccgtcgggg ccgggtgtcc
     8041 acaggtcccg gtgctcgccg ggcacccgct cgggaatgcc gtgcgtcggg ggcaactcgg
     8101 tgccggcggt ggagaccatg cggggcggtg tcgcggtgtc cgtccagagg acgtgcacat
     8161 cggacccggc acgcggcgcc gcatcgagaa gggtcccgcg cggtgtcggt acgggctcga
     8221 cggtgcgctc cgcgaggtgt acgcggtgca gggtgctgcg gccgtgccgg tcctggcgga
     8281 ccaggagcga gtgtccttcg gggtgccagc gagcggtgat ctcggtgtcg aaggcgcacc
     8341 aggagtgggt gacgagtccg gtgcccgggg tccaactcgc cagcaggtag cggttgttgg
     8401 actcccggac gagtaacagt tcggcatgtc gcgcggtggg cgcgaagccc agcgaccaga
     8461 gccggccccc gcccccgtcc ccggcgagca ccgcgcactg ggtgccgtcg tcggcgagta
     8521 cggtcacggc ccgtgccgat ccggccgcgc cgccgagggc gaggaggcgg ccgtcggggg
     8581 tgatcccgga cagggtggcg tgcccgtcga cgcgcgtcac ttcgcgggcg gcaccgccac
     8641 gcgggccgag gtacacggtg gtggagcggg cgtcgccgat gcccatggcc acgcgtccgc
     8701 cgtcgctcac agcgaggccg cgcggctcgc cctcgggcac gccgagcagc cccggcaggt
     8761 cggggccgcc ctcgaacggc tggaaccgcc agcgcccgga tccgtggtcg tcctcgtcga
     8821 accaccacac ccgggcgtcg cggtcgatcg cgccgtgcag ggtgccgtgc ggacggtcgg
     8881 tgacctggcg ggcccggcgg gtgtccgcgt cccaggtgaa cacttcgcaa cgtccgtcgg
     8941 cgtcggcggt gaacaccatc ttggcgggct gggcggcaca cacctcgggc agggccgcgc
     9001 ggtagacctg gtagctcctg ctcatgccgt cgcccccgtg agccgggacc gtacgacggt
     9061 ggtccgccag gcgagggcgg tgagcagtcc tgcgcagatc acggcgggcg cggccgtccc
     9121 gacgcgggtc acgaacaccc cggccacgac cggcgcgagt gcggccgacc caccgacggc
     9181 gctcgcgagg accgcgccgg tgcgggcctg cagggagggc ggcgtctcct gcagggcacg
     9241 ggactggagc acgaccgcga ccagtggcat caggaggcag aacaccccga acaacgcgcc
     9301 gtacgtccag ggtccgtcgg cggtggcgag gccggccgcg agcgggacga gcagccagga
     9361 caccgtcacc agcagccgcg cggcaccgat gcgccccacg acacgcggcg cggccagcga
     9421 cccgacgagg cccgcggcgc cggacagcgc caggacgagg cccatgggcg cgccgccgtg
     9481 accgcccttc tgcagggtga acaccgcgcc gtagtagagc gcgacgatca ccccgttcac
     9541 ggtggcggtc caggtcacga cgagccggag gaggggcgag gtgcggacga gagcgagtcc
     9601 tgcgccgagt tcgctccgaa gactccgaag tcccctgtcg ggtacgggag ttgaggtagg
     9661 tccggcggcc gcttcggcca cccgcgccga gccgttcgcc gcggtcccgg gggccgggtc
     9721 gcgcacgggc cgcaggtccg agcgcagcgc ccgtacgcat ccggcggcga cggcgtagga
     9781 gacggcgtcc gcgaggaagg gcagggcgcg ggccagctgg tagagcgcac cgccgagggc
     9841 cgggcccagg atcagcgcac cctggtcgcc cgccgcaagc ccggccacca cgcgggggca
     9901 gtcggcggcc ggggcgatca tggccacggt gccggccgcg gcggcctcgt acaccgcggt
     9961 cgagagccgc tccacgagga ccgcgccgag cacgagggcg agcggtggct cccccgtggc
    10021 caccgccgcc gtcaccccgg ccatggcgag cgccgagacc gtcgcggacc agaacatcac
    10081 ggccttgcgg gacccccggt cggccggaac ggccgccagc ggggccagca gcagcccggc
    10141 cgccgtggac acccccgcga cgaggccgac cgtcgcggcg gaatggccga ggcccagcag
    10201 cagcaacggc agtacgacac cggaggcctg ggtgcccagg gcgtcggagg cgctcgccca
    10261 ccagaacagc cggacgtcac ggggcaccgg ccggcgggcc gcccgtcgct cggcggcggt
    10321 ggtgccggtg gtcatcactt tcccgcccct gaagaggtcc ccgggagacc cgggaacgcc
    10381 ggttcgcgca cagccacccc acagttggtc aagttgcgaa gaacatgtgc aggaatcgaa
    10441 acgagcggga gcctaatcag acgcccgctc ggcccacaag agtcgcgccg ccgaggccgg
    10501 aatcgaactg tgtaagggcc cggcaaagta accgattact tgatccgcgg ttcggcccgc
    10561 ccgcgggtcg gcccccaaag ggacgaaagg gaaagagtgc cgccgctcgt cgcgctcccg
    10621 gatgtcttcg cgctccctcc gcgcccgctt cgcggcggtg aacctccttg ccctggtggc
    10681 gagttcgcgc actccgcccg gcatatgcac ctttccggcg gcgaaccggt gaccaccacc
    10741 gcgccccgga cgcgtcacga tcggcgggtc cgccgcatcc gggtcccgcg ggcggcatca
    10801 cgtccccgcc caccccgcga cgaccccggc gcggaacggt tccatccgtc ggactcattg
    10861 gccggaaacg agcgcgcccg gcgaccggag cccggcgttc accgaagctc gaaagagacc
    10921 tctgattgat caactctgct catcgcaagg gcagttgcgc gatcaggagg cgtcccggca
    10981 gccggtccaa cgaccgctgc gcgaagccgg gttgagcgaa gcaccgagcc cggcatgccc
    11041 gcgccccctc acccgtgggg gcgagggggc gcgggaacgg atgccggcag cagcgtcact
    11101 gcggggacgg gctgctgctc gccatggcgt ggcactcgcc gccctccacc atcttcaggt
    11161 gcgcggcgac gatcgggcgg gcctccttgg cggccgcctg gatctgggcg tcgttaccgg
    11221 acttcagctc cttgtcgatc agcgcgagcg cctgctggtg gccgctgctc tggaccttga
    11281 gccaggcggt gtcgtaggcc gcggtgccgt tcatcggctt cagcgcggac agcgacttct
    11341 gctgcgccgc ggtggactcc ttcggcaggg tgatgttctt ggtcttggcg agagccgcgc
    11401 ccttggcgtc gagcttggtg tggtcgcgca cgatgatggc cgagacctgt ttcacacagc
    11461 tgctgctcgc atgcgtagcg gcgtccttgc ccgcggctat ctcggccagg ttcgcctggt
    11521 ggatggcctg caggaacacc gcgtccgcag tggcggaggg gctggccgca ccgaaggcgg
    11581 ccgtggttcc ggtgagggtc agcgccgcgg cggcggccgt agtggcgatg tgggtgatgc
    11641 gcatgggtac ctccacgtgt ccgtggatcg ggtaggggcc gcttcggcat ccggaccgcg
    11701 ctcgacgcct gtccgggcag ggcccgaccc tcgtcatcct aggtgtgctc ctcactgcgc
    11761 gcctctccag atcactcccc gtaaccatcg tgcggagggc cccgcgatcc gccccgtgaa
    11821 ccgatggacg aaggccccgc cgtggggtca gggtgccggg tagggggccg ccttgaggcg
    11881 ccgggcgagg tggctcgtat gggcggctag ggtggcggtg gtctccgcgg tggacttc
//
