LOCUS       NZ_BMUT01000030        13052 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces hiroshimensis strain JCM 4586 sequence30, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUT01000030
VERSION     NZ_BMUT01000030.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces hiroshimensis
  ORGANISM  Streptomyces hiroshimensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 13052)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 90X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650335.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:32:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,830
            CDSs (total)                      :: 6,745
            Genes (coding)                    :: 6,620
            CDSs (with protein)               :: 6,620
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 25 of 125
            Pseudo Genes (incomplete)         :: 107 of 125
            Pseudo Genes (internal stop)      :: 4 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:07:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 13052
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUT01000030.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..13052
                     /culture_collection="JCM:4586"
                     /db_xref="taxon:66424"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces hiroshimensis"
                     /strain="JCM 4586"
                     /submitter_seqid="sequence30"
                     /type_material="type strain of Streptomyces hiroshimensis"
     region          1..13052
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS-like"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds((PP-binding or NAD_binding_4) and (AMP-binding
                     or A-OX) and not alpha_am_amid)"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..13052
                     /SMILES="C1=CC(=O)C(N)C=C1C(=O)C=CC(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="chemical_hybrid"
                     /product="NRPS-like"
                     /product="T1PKS"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    1..13052
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[3260:11645](-)"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(3261..11645)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="0"
                     /detection_rule="cds((PP-binding or NAD_binding_4) and
                     (AMP-binding or A-OX) and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS-like"
                     /protocluster_number="1"
     protocluster    1..13052
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[3260:11645](-)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      complement(3261..11645)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="2"
     gene            <74..253
                     /locus_tag="IE241_RS34860"
                     /old_locus_tag="GCM10010324_68820"
                     /pseudo=""
     CDS             <74..253
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070427.1"
                     /locus_tag="IE241_RS34860"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68820"
                     /product="transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="PASAPKPSRPGPGRPPGSKNRLPATRHDVGRVLATGEAYSRPAHH
                     KKGTKPRRTEERQT"
     gene            324..833
                     /locus_tag="IE241_RS33780"
                     /old_locus_tag="GCM10010324_68830"
     CDS             324..833
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056374.1"
                     /locus_tag="IE241_RS33780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68830"
                     /product="hypothetical protein"
                     /protein_id="WP_229900229.1"
                     /transl_table=11
                     /translation="MIIAVAGVAGTLGGALLTQRGSERAKRLEIELVRDHEEVREARSL
                     RRTCYVELNRDARQFTTALNRHLHVMRERGVEEADSDALEEAKNAHRDRYSEAQMIAPE
                     EVLRRASLVNQALNKVYGQVKRLERGAPEPGETMETAAQAQYEVWEMLRTMRTAMRHDL
                     GVSHED"
     gene            complement(938..1786)
                     /locus_tag="IE241_RS33785"
                     /old_locus_tag="GCM10010324_68840"
     CDS             complement(938..1786)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047900.1"
                     /locus_tag="IE241_RS33785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68840"
                     /product="serine/threonine protein kinase"
                     /protein_id="WP_190025703.1"
                     /transl_table=11
                     /translation="MASHPLLDVTELPSVEAHLERIGTVFASFRDQDSGCVSYGVRLPG
                     GERWFVKEGTSDGARRSLCRAWAFHRAVSHPVIVPQVHRIAVRGNWAVVMPWHDGEVLY
                     HPTTGGTADRTAPGSAMARFRALPLSLVMRAWDRILSAHLAVEEAGHVAVDFYDGALLY
                     DFTGDTMRLVDLDEYRPGPFVLEADRLPGSRRFMAPEEYERGATIDARTTVFVLGRAAR
                     LLLDAGDDERAWRGGPARLKVLHRATRADPGERFASVRRFAAAWHAAGSAPEGSPRPGT
                     P"
     gene            complement(1799..2464)
                     /locus_tag="IE241_RS33790"
                     /old_locus_tag="GCM10010324_68850"
     CDS             complement(1799..2464)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962798.1"
                     /locus_tag="IE241_RS33790"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68850"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_190025704.1"
                     /transl_table=11
                     /translation="MPRTKGDHEARRRDVSEAVWRVLAAHGFGGLTLRAVATELGATTG
                     LLTHYFPAKRDLVAHALDVLEQRSASRPRRAAGPGLPAVRAALLDILPLTAEATDANRI
                     WVSSWDVALADPALSGDHARRYAQSRGKLRDLVATAQELGELPAGDPELIAAGAQSFAL
                     GLVVQALFAPASFPPQRQVELLDDYLASLASPRAAMSPASPSSSRGEDPAPPGRHPGR"
     misc_feature    2505..2519
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.48"
                     /tool="antismash"
     gene            2513..3073
                     /locus_tag="IE241_RS33795"
                     /old_locus_tag="GCM10010324_68860"
     CDS             2513..3073
                     /EC_number="2.3.-.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /GO_function="GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1111: GCN5-related N-acetyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003962799.1"
                     /locus_tag="IE241_RS33795"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68860"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_190025705.1"
                     /transl_table=11
                     /translation="MFSLFLRDNACLEPLEIWHAEEFAAHLDRAREHIRPWVGQAFVTD
                     DLDGARATLRRYAERQAEDGARLYGIRLDGVLVGGVMFTDFSTAAGSCEIGCWLEPAAE
                     GHGLVTDACSALLDWAFTSRGLHRAEWHCRADNDRSSAVAVRLGMALEGVRRQSWPYGG
                     VRHDKQIWAILAPEWLRTGSRAA"
     gene            complement(3261..11645)
                     /locus_tag="IE241_RS33800"
                     /old_locus_tag="GCM10010324_68870"
     CDS             complement(3261..11645)
                     /NRPS_PKS="Domain: CAL_domain (36-450). E-value: 9.3e-165.
                     Score: 541.3. Matches aSDomain:
                     nrpspksdomains_IE241_RS33800_CAL_domain.1"
                     /NRPS_PKS="Domain: PKS_PP (536-622). E-value: 9.2e-19.
                     Score: 59.6. Matches aSDomain:
                     nrpspksdomains_IE241_RS33800_PKS_PP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (659-1090). E-value:
                     7.2e-172. Score: 564.0. Matches aSDomain:
                     nrpspksdomains_IE241_RS33800_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (1219-1509). E-value: 1.1e-97.
                     Score: 319.0. Matches aSDomain:
                     nrpspksdomains_IE241_RS33800_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (1611-1779). E-value: 3.3e-51.
                     Score: 165.9. Matches aSDomain:
                     nrpspksdomains_IE241_RS33800_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (2106-2283). E-value: 4.9e-61.
                     Score: 197.8. Matches aSDomain:
                     nrpspksdomains_IE241_RS33800_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (2394-2480). E-value: 2.9e-26.
                     Score: 83.6. Matches aSDomain:
                     nrpspksdomains_IE241_RS33800_PKS_PP.2"
                     /NRPS_PKS="Domain: Thioesterase (2562-2784). E-value:
                     1.9e-24. Score: 79.3. Matches aSDomain:
                     nrpspksdomains_IE241_RS33800_Thioesterase.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: PP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     NRPS-like: AMP-binding"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Polysacc_synt_2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Thioesterase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020124227.1"
                     /locus_tag="IE241_RS33800"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68870"
                     /product="type I polyketide synthase"
                     /protein_id="WP_190025706.1"
                     /sec_met_domain="AMP-binding (E-value: 1.2e-105, bitscore:
                     342.2, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 6e-217, bitscore: 709.3,
                     seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 5.6e-91, bitscore:
                     293.4, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 1.2e-98, bitscore: 319.0,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 1.6e-64, bitscore:
                     206.0, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="Polysacc_synt_2 (E-value: 3.3e-08,
                     bitscore: 21.8, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3e-11, bitscore:
                     32.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="PF00561 (E-value: 1.1e-09, bitscore: 27.1,
                     seeds: 48, tool: rule-based-clusters)"
                     /sec_met_domain="Thioesterase (E-value: 5.3e-20, bitscore:
                     61.4, seeds: 16, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEIHGELMRALPGVLKAHSMQIAGKIAFRDAQRGVTYGELEKRT
                     GRLAGHLARLGVRRGDRVALHLGSRVEMVEGFLAVLRAGAVGVPLDPGATEAELAYFLD
                     DSGAVAVITEESRLSRVAGLAATRPRLHARVVVAGTDAVPDGAPAAEHLFEQLAGTDPG
                     QAPRDDLGLDEPAWILYTSGTTGRSKGVVSSQRTALWSVAAAYVPLWGLERQDRLLWPL
                     PMSHAYAHSLCLLGVISVGASAYLLGRGASVAQALAEQPCTVVAGVPATYHLLTEALRE
                     ASRPRRPSGVRLCVTAGAPCPPALRAEVEELLGAPLLDGYGSTETCGKIAVDRPGGPPE
                     GRSGVLPVPGVEVRLTDPAGGDDVADGTDGEIWVRGPNLMLGYHNRPDDTAQAFRDGWY
                     RTGDLGRRGDGGRLQVTGRVKELIIRGGENVNPVEVEHVLLGCPGVADAAVVGRPHDVL
                     GEVPVAFVVPGPHGVDTDRVRAACRERLSGFKVPDEVYETGSVPRTPSGKTDRPALARQ
                     LAERLGAVREDAVSALRRRLLSLPPSGRYDAVLRLVLARTADAGGAPEQETEPEPGRPF
                     TASGITSLGGVLLRDRLGADTGLELPATLVFDHPTPAAVAEFLHSALLPGRAPAARTRG
                     AKARTPGAKSRTAGAKSRTASGDPVAIVSMACRYPGLRDDVTSPEELWQLVADGADATS
                     DFPGDRGWDPVHLFDPDPDRLGASVTRRGGFLRLAADFDAAFFGMSPREALATDPQQRL
                     LLETTWELCERARIDPADLRGSDTGVFVGVMHDDYAGRLLHGGAHPLEAHLALGSTGSV
                     ASGRIAYTLGLHGPAVTVDTACSSSLVAMHWAAQALRSGECSLAVAGGVTVMATPAPFV
                     AFSRLRGLAPDGRCKPFSAAADGTGWAEGAGVVLLERLSDARRNGHPVLAVLRGSAVGS
                     DGASNGLTAPSGTAQQQVVRQALADAGLSPADIDTVETHGTGTALGDPVEARALLAAYG
                     QERSAGRPLWLGALKSNIGHTQAAAGVGGVIKTVLAMRHGRLPRILHCEEPSPRVDWSS
                     GTVRLLTREEEWHPAPGSPRRAGVSAFGIGGTNAHVILEEAPWETEPGAEGDAGTAPGR
                     PQAVPPLLLSGADPRALRAQAGQVAALLRQHPRPPLLDVAFSLATTRTALAHRAAVTGA
                     DHTALLRALDAQAAAGTGSGSGSGSGSGSGSNGGNGGSSPTARPATSSGRLALLFSGQG
                     AQRPGMGRELHGAFPAFAAAFDSLCERFDAEGGLERPLRTVLWAEAGSDDATLSARTDF
                     AQAGLFVFEVALFRLLESWGVRPDVLSGHSVGELAVAHVAGVLSEPDAVELVAARGRLM
                     RQLPGSGAMVALEATEREAEQELTGWADRVAVAAVNGPRSVVLSGEEGAVLTVAARFAG
                     RGRRTTRLQVGHAFHSPLMQPMLTAFGHVASRLTYRAPRIPVMSTLTGRPAAGDDLSAA
                     YWVRHARETVRFGDAVHRLVHDERVTVFAEVGPGAQLTAAAMSGLAVDGKKPDEGSTEG
                     PVEGPGEGPVFIATARDGAREAEAVLAALGRLHVHGLPVDWHRVYEGSGARRVDLPTYP
                     FQRRRYWLDAPASPSPVPAPAPAPAPATAGSGHPLLPQTTTVPGTERLLCTGHLSTVAQ
                     PWLRDHVVAGHVLVPAAAFAEMVLHAGDACGLETAEDFALLAPLLLPGSGERVQVQVVL
                     GEPDASGRRTADVHSRPQESGPLGGWTHHATGRLGPAAGAGDERRGATTAWPPPGAVPV
                     DLTGAYERLADAGHAYGLAFQGVTAVWRGPDEEVEEVYAEVRLPEAAGAEARRYGIHPA
                     LLDAALHAALLAAPSAGPARLPFAWRGLTVHAVGATALRVRIRATGTDTVAVDLADPAG
                     QPVARVESLTTRPLTESVDAAAVGMNGRGGLYTLRWTGVPDGGEELSLSVAAPDDLGLR
                     SVLPATPALQGKAPEAVVVSLSSGSEPSSRSEPSSESEPSSGSEPSSGTDPVSATHRLT
                     ARALTLLQTYLSVPAAPGTRLVLVTRGATAPVPDLSAAAVWGLVRSAQAEYPGRLTLVD
                     TDGSPESLRLLPAAVATGEPQLSLVDGTARVPRLAEAETLSLTHSPTADGFASGVGTVL
                     VTGGTGALGGLLARHLVTRYGVRHLVLVSRRGPQSAGARQLQAVLEEAGARADVIACDV
                     AERAQLAAVVEKYAPQLSAVVHAAGVLDDGVLEALTPERFAAALRPKADAAWHLHELTR
                     HLPLSHFVLFSSAAGLLGNPGQANYAAANAFLDALAHHRTSLGLPALSLVWGPWAGGEG
                     MAARTDGAERAMGGVLRAVSPAQGLAMFDAALGEGGPVLAPLLPDRSRPAAPAGSPVPP
                     PLRDLLRPARPARPAAGSVGDAGSVGATGSKAAAGNGPEDATGSWRERLAALPEADRAL
                     ALLELVRAEVAAVLGHPDAAAVAPGRPFTELGFDSLTGVLLRNRLAVLTGLTLPATVTF
                     DRPSAAELAGHLYEELGGDLLAGTAAAAGPDVHGRARPPQTLASLYRRVCGTGDVVSAM
                     HLLVTASLALPSFGPADSSRHELAPVKLAEDVQGTGRPALVCFPGFSPNLGRPWYAALA
                     AAFAGERDVFEFRHPGAAHGDTVPRDWRTLVDLHADTVRTHLGDRPYAVLGYSMGGYPA
                     HSVTARLAATDTPPVGLVIADTYHVTPDREEEPWLLAMPARLPLQLGERFDTAVDDMSM
                     AALGAYTRMARGWHPEPLDVPTLLIRAAEPLPPVRPGTGDGTREYGPGRPVWPAPHTAV
                     EVPGDHWTLFEEHVRTTADAIRAWLGAQVSATGRR"
     aSDomain        complement(3294..3959)
                     /ASF="active site serine inconclusive"
                     /aSDomain="Thioesterase"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE241_RS33800_Thioesterase.1"
                     /evalue="1.90E-24"
                     /label="IE241_RS33800_Thioesterase.1"
                     /locus_tag="IE241_RS33800"
                     /protein_end="2784"
                     /protein_start="2562"
                     /score="79.3"
                     /tool="antismash"
                     /translation="LVCFPGFSPNLGRPWYAALAAAFAGERDVFEFRHPGAAHGDTVPR
                     DWRTLVDLHADTVRTHLGDRPYAVLGYSMGGYPAHSVTARLAATDTPPVGLVIADTYHV
                     TPDREEEPWLLAMPARLPLQLGERFDTAVDDMSMAALGAYTRMARGWHPEPLDVPTLLI
                     RAAEPLPPVRPGTGDGTREYGPGRPVWPAPHTAVEVPGDHWTLFEEHVRTTADAIRAWL
                     "
     aSModule        complement(3294..9668)
                     /complete
                     /domains="nrpspksdomains_IE241_RS33800_PKS_KS.1"
                     /domains="nrpspksdomains_IE241_RS33800_PKS_AT.1"
                     /domains="nrpspksdomains_IE241_RS33800_PKS_DH.1"
                     /domains="nrpspksdomains_IE241_RS33800_PKS_KR.1"
                     /domains="nrpspksdomains_IE241_RS33800_PKS_PP.2"
                     /domains="nrpspksdomains_IE241_RS33800_Thioesterase.1"
                     /final_module
                     /locus_tags="IE241_RS33800"
                     /monomer_pairings="mal -> ccmal"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       complement(3702..3770)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0019"
                     /evalue="4.80E-05"
                     /label="NRPS-te1"
                     /locus_tag="IE241_RS33800"
                     /protein_end="2648"
                     /protein_start="2625"
                     /score="15.9"
                     /tool="antismash"
                     /translation="DRPYAVLGYSMGGYPAHSVTARL"
     aSDomain        complement(4206..4463)
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE241_RS33800_PKS_PP.2"
                     /evalue="2.90E-26"
                     /label="IE241_RS33800_PKS_PP.2"
                     /locus_tag="IE241_RS33800"
                     /protein_end="2480"
                     /protein_start="2394"
                     /score="83.6"
                     /tool="antismash"
                     /translation="LAALPEADRALALLELVRAEVAAVLGHPDAAAVAPGRPFTELGFD
                     SLTGVLLRNRLAVLTGLTLPATVTFDRPSAAELAGHLYEEL"
     CDS_motif       complement(4365..4421)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0018"
                     /evalue="1.80E+01"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="IE241_RS33800"
                     /protein_end="2427"
                     /protein_start="2408"
                     /score="-1.3"
                     /tool="antismash"
                     /translation="ELVRAEVAAVLGHPDAAAV"
     CDS_motif       complement(4488..4511)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0017"
                     /evalue="5.70E+01"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="IE241_RS33800"
                     /protein_end="2386"
                     /protein_start="2378"
                     /score="-2.1"
                     /tool="antismash"
                     /translation="AAGNGPED"
     aSDomain        complement(4797..5327)
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE241_RS33800_PKS_KR.1"
                     /evalue="4.90E-61"
                     /label="IE241_RS33800_PKS_KR.1"
                     /locus_tag="IE241_RS33800"
                     /protein_end="2283"
                     /protein_start="2106"
                     /score="197.8"
                     /specificity="KR activity: active"
                     /specificity="KR stereochemistry: B1"
                     /tool="antismash"
                     /translation="GTVLVTGGTGALGGLLARHLVTRYGVRHLVLVSRRGPQSAGARQL
                     QAVLEEAGARADVIACDVAERAQLAAVVEKYAPQLSAVVHAAGVLDDGVLEALTPERFA
                     AALRPKADAAWHLHELTRHLPLSHFVLFSSAAGLLGNPGQANYAAANAFLDALAHHRTS
                     LGLPALSLVWGPWA"
     CDS_motif       complement(4845..4952)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0016"
                     /evalue="6.40E-23"
                     /label="PKSI-KR_m4"
                     /locus_tag="IE241_RS33800"
                     /protein_end="2267"
                     /protein_start="2231"
                     /score="73.4"
                     /tool="antismash"
                     /translation="LSHFVLFSSAAGLLGNPGQANYAAANAFLDALAHHR"
     misc_feature    complement(5007..5009)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS_motif       complement(5223..5324)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0015"
                     /evalue="2.70E-09"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE241_RS33800"
                     /protein_end="2141"
                     /protein_start="2107"
                     /score="29.6"
                     /tool="antismash"
                     /translation="TVLVTGGTGALGGLLARHLVTRYGVRHLVLVSRR"
     aSDomain        complement(6309..6812)
                     /ASF="catalytic triad H,G,P inconclusive"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE241_RS33800_PKS_DH.1"
                     /evalue="3.30E-51"
                     /label="IE241_RS33800_PKS_DH.1"
                     /locus_tag="IE241_RS33800"
                     /protein_end="1779"
                     /protein_start="1611"
                     /score="165.9"
                     /tool="antismash"
                     /translation="HPLLPQTTTVPGTERLLCTGHLSTVAQPWLRDHVVAGHVLVPAAA
                     FAEMVLHAGDACGLETAEDFALLAPLLLPGSGERVQVQVVLGEPDASGRRTADVHSRPQ
                     ESGPLGGWTHHATGRLGPAAGAGDERRGATTAWPPPGAVPVDLTGAYERLADAGHAYGL
                     AFQGV"
     CDS_motif       complement(6654..6728)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0014"
                     /evalue="2.60E-09"
                     /label="PKSI-DH_m2"
                     /locus_tag="IE241_RS33800"
                     /protein_end="1664"
                     /protein_start="1639"
                     /score="29.5"
                     /tool="antismash"
                     /translation="WLRDHVVAGHVLVPAAAFAEMVLHA"
     aSDomain        complement(7119..7988)
                     /ASF="Malonyl-CoA specific"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE241_RS33800_PKS_AT.1"
                     /evalue="1.10E-97"
                     /label="IE241_RS33800_PKS_AT.1"
                     /locus_tag="IE241_RS33800"
                     /protein_end="1509"
                     /protein_start="1219"
                     /score="319.0"
                     /specificity="substrate consensus: mal"
                     /specificity="PKS signature: Malonyl-CoA"
                     /specificity="Minowa: mal"
                     /tool="antismash"
                     /translation="LFSGQGAQRPGMGRELHGAFPAFAAAFDSLCERFDAEGGLERPLR
                     TVLWAEAGSDDATLSARTDFAQAGLFVFEVALFRLLESWGVRPDVLSGHSVGELAVAHV
                     AGVLSEPDAVELVAARGRLMRQLPGSGAMVALEATEREAEQELTGWADRVAVAAVNGPR
                     SVVLSGEEGAVLTVAARFAGRGRRTTRLQVGHAFHSPLMQPMLTAFGHVASRLTYRAPR
                     IPVMSTLTGRPAAGDDLSAAYWVRHARETVRFGDAVHRLVHDERVTVFAEVGPGAQLTA
                     AAMSGLAVD"
     CDS_motif       complement(7227..7262)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0013"
                     /evalue="1.60E-04"
                     /label="PKSI-AT-M_m8"
                     /locus_tag="IE241_RS33800"
                     /protein_end="1473"
                     /protein_start="1461"
                     /score="15.1"
                     /tool="antismash"
                     /translation="YWVRHARETVRF"
     CDS_motif       complement(7482..7520)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0012"
                     /evalue="5.80E-04"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="IE241_RS33800"
                     /protein_end="1388"
                     /protein_start="1375"
                     /score="13.1"
                     /tool="antismash"
                     /translation="AAVNGPRSVVLSG"
     CDS_motif       complement(7617..7736)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0011"
                     /evalue="1.60E-19"
                     /label="PKSI-AT-M_m3"
                     /locus_tag="IE241_RS33800"
                     /protein_end="1343"
                     /protein_start="1303"
                     /score="62.1"
                     /tool="antismash"
                     /translation="GVRPDVLSGHSVGELAVAHVAGVLSEPDAVELVAARGRLM"
     CDS_motif       complement(7938..7985)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0010"
                     /evalue="3.90E-05"
                     /label="PKSI-AT-M_m1"
                     /locus_tag="IE241_RS33800"
                     /protein_end="1236"
                     /protein_start="1220"
                     /score="16.3"
                     /tool="antismash"
                     /translation="FSGQGAQRPGMGRELH"
     aSDomain        complement(8376..9668)
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE241_RS33800_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="7.20E-172"
                     /label="IE241_RS33800_PKS_KS.1"
                     /locus_tag="IE241_RS33800"
                     /protein_end="1090"
                     /protein_start="659"
                     /score="564.0"
                     /tool="antismash"
                     /translation="VAIVSMACRYPGLRDDVTSPEELWQLVADGADATSDFPGDRGWDP
                     VHLFDPDPDRLGASVTRRGGFLRLAADFDAAFFGMSPREALATDPQQRLLLETTWELCE
                     RARIDPADLRGSDTGVFVGVMHDDYAGRLLHGGAHPLEAHLALGSTGSVASGRIAYTLG
                     LHGPAVTVDTACSSSLVAMHWAAQALRSGECSLAVAGGVTVMATPAPFVAFSRLRGLAP
                     DGRCKPFSAAADGTGWAEGAGVVLLERLSDARRNGHPVLAVLRGSAVGSDGASNGLTAP
                     SGTAQQQVVRQALADAGLSPADIDTVETHGTGTALGDPVEARALLAAYGQERSAGRPLW
                     LGALKSNIGHTQAAAGVGGVIKTVLAMRHGRLPRILHCEEPSPRVDWSSGTVRLLTREE
                     EWHPAPGSPRRAGVSAFGIGGTNAHVILEEAP"
     CDS_motif       complement(8601..8645)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0009"
                     /evalue="1.10E-02"
                     /label="PKSI-KS_m6"
                     /locus_tag="IE241_RS33800"
                     /protein_end="1015"
                     /protein_start="1000"
                     /score="8.8"
                     /tool="antismash"
                     /translation="GALKSNIGHTQAAAG"
     CDS_motif       complement(8706..8750)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0008"
                     /evalue="2.70E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="IE241_RS33800"
                     /protein_end="980"
                     /protein_start="965"
                     /score="10.4"
                     /tool="antismash"
                     /translation="VETHGTGTALGDPVE"
     CDS_motif       complement(9126..9173)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0007"
                     /evalue="1.50E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE241_RS33800"
                     /protein_end="840"
                     /protein_start="824"
                     /score="20.1"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     aSDomain        complement(9780..10037)
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE241_RS33800_PKS_PP.1"
                     /evalue="9.20E-19"
                     /label="IE241_RS33800_PKS_PP.1"
                     /locus_tag="IE241_RS33800"
                     /protein_end="622"
                     /protein_start="536"
                     /score="59.6"
                     /tool="antismash"
                     /translation="LLSLPPSGRYDAVLRLVLARTADAGGAPEQETEPEPGRPFTASGI
                     TSLGGVLLRDRLGADTGLELPATLVFDHPTPAAVAEFLHSA"
     aSModule        complement(9780..11537)
                     /complete
                     /domains="nrpspksdomains_IE241_RS33800_CAL_domain.1"
                     /domains="nrpspksdomains_IE241_RS33800_PKS_PP.1"
                     /locus_tags="IE241_RS33800"
                     /monomer_pairings="AHBA -> AHBA"
                     /starter_module
                     /tool="antismash"
                     /type="pks"
     CDS_motif       complement(10248..10316)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0006"
                     /evalue="3.70E+01"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="IE241_RS33800"
                     /protein_end="466"
                     /protein_start="443"
                     /score="-2.3"
                     /tool="antismash"
                     /translation="GVADAAVVGRPHDVLGEVPVAFV"
     aSDomain        complement(10296..11537)
                     /aSDomain="CAL_domain"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE241_RS33800_CAL_domain.1"
                     /evalue="9.30E-165"
                     /label="IE241_RS33800_CAL_domain.1"
                     /locus_tag="IE241_RS33800"
                     /protein_end="450"
                     /protein_start="36"
                     /score="541.3"
                     /specificity="Minowa: AHBA"
                     /tool="antismash"
                     /translation="TYGELEKRTGRLAGHLARLGVRRGDRVALHLGSRVEMVEGFLAVL
                     RAGAVGVPLDPGATEAELAYFLDDSGAVAVITEESRLSRVAGLAATRPRLHARVVVAGT
                     DAVPDGAPAAEHLFEQLAGTDPGQAPRDDLGLDEPAWILYTSGTTGRSKGVVSSQRTAL
                     WSVAAAYVPLWGLERQDRLLWPLPMSHAYAHSLCLLGVISVGASAYLLGRGASVAQALA
                     EQPCTVVAGVPATYHLLTEALREASRPRRPSGVRLCVTAGAPCPPALRAEVEELLGAPL
                     LDGYGSTETCGKIAVDRPGGPPEGRSGVLPVPGVEVRLTDPAGGDDVADGTDGEIWVRG
                     PNLMLGYHNRPDDTAQAFRDGWYRTGDLGRRGDGGRLQVTGRVKELIIRGGENVNPVEV
                     EHVLLGCPGVADAAV"
     CDS_motif       complement(10464..10550)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0005"
                     /evalue="4.80E-10"
                     /label="NRPS-A_a6"
                     /locus_tag="IE241_RS33800"
                     /protein_end="394"
                     /protein_start="365"
                     /score="31.7"
                     /tool="antismash"
                     /translation="DGTDGEIWVRGPNLMLGYHNRPDDTAQAF"
     CDS_motif       complement(11019..11048)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0004"
                     /evalue="9.90E+00"
                     /label="NRPS-A_a2"
                     /locus_tag="IE241_RS33800"
                     /protein_end="209"
                     /protein_start="199"
                     /score="0.6"
                     /tool="antismash"
                     /translation="WSVAAAYVPL"
     CDS_motif       complement(11064..11120)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0003"
                     /evalue="7.70E-06"
                     /label="NRPS-A_a3"
                     /locus_tag="IE241_RS33800"
                     /protein_end="194"
                     /protein_start="175"
                     /score="18.6"
                     /tool="antismash"
                     /translation="AWILYTSGTTGRSKGVVSS"
     CDS_motif       complement(11373..11414)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0002"
                     /evalue="7.00E-03"
                     /label="NRPS-A_a2"
                     /locus_tag="IE241_RS33800"
                     /protein_end="91"
                     /protein_start="77"
                     /score="10.1"
                     /tool="antismash"
                     /translation="LAVLRAGAVGVPLD"
     CDS_motif       complement(11460..11513)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS33800_0001"
                     /evalue="1.40E+01"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE241_RS33800"
                     /protein_end="62"
                     /protein_start="44"
                     /score="-1.5"
                     /tool="antismash"
                     /translation="TGRLAGHLARLGVRRGDR"
     misc_feature    complement(11672..11686)
                     /note="TFBS match to CelR, Cellobiose uptake repressor,
                     confidence: weak, score: 22.58"
                     /tool="antismash"
     gene            11829..12920
                     /locus_tag="IE241_RS33805"
                     /old_locus_tag="GCM10010324_68880"
     CDS             11829..12920
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018552502.1"
                     /locus_tag="IE241_RS33805"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68880"
                     /product="ABC transporter permease"
                     /protein_id="WP_190025707.1"
                     /transl_table=11
                     /translation="MGEESGAAGTPHGPAAPTGRRHRARHRWSAFRHSPFFPAAVLVLI
                     VAAAAGLFAGSYTYAMANPTPHNIPTAVTGVPESEIKRAEFVQGMERALGAELLLRHFA
                     TYEDARRAVEDQRVYAILQDRHHAAELDVSGASGATVAQLLTETAPRVGKEISVLVVVR
                     DVNPLQKGDPRGLALFYITLAAVIVGFVGAIQMSVHAGELNPAERIAFTAAYSTLGGFT
                     IAAVVDWGLGAVRLPFAESWLILALTMFTSGMVFTMFNALFGRWAMLPTWGLMVLLGNP
                     SSGGAVSWPLLPSLLGEIGRWLPPGASVNAQHTAVYFRQHQHFFPFAVLIAWAALSCAI
                     FWTWRHRHPGGRDTAAAAAVSAG"
ORIGIN
        1 gatgatcgaa accgaacagc ggctcgctct tgatcatcgc caggaggaag tcgtgcgaca
       61 gcctcccggc tccccggcca gtgcaccgaa accgtctcgt ccggggcctg gccggccgcc
      121 gggttcgaag aaccgcctcc cagccacccg tcatgacgtg ggcagagtcc tggccaccgg
      181 cgaggcatac agccgccccg cccaccacaa aaagggcacc aaaccccggc gcaccgagga
      241 gcggcaaact tgacggcctg ggccatgtgc agcgggcacg aggccgaaga atttcacggg
      301 gtacgtcggt ggatctcagc tcagtgatca tcgctgtcgc aggcgtcgca ggaaccctgg
      361 gaggggcgct actcacccag cgcggctccg aacgagcgaa gcgcctggag atagagctcg
      421 ttcgagacca tgaggaggtt cgcgaggccc gttcgctgcg acgcacctgc tatgtggaac
      481 tcaaccggga tgctcggcag ttcaccaccg ctctcaaccg ccacctgcac gtcatgaggg
      541 aacgcggcgt ggaggaggcc gacagcgacg cactcgagga agcgaagaac gcgcaccgcg
      601 atcgatactc cgaagcacag atgatcgccc ccgaagaagt actcaggcga gcgagtctcg
      661 tcaaccaggc cctgaacaag gtctacggcc aggtcaagcg cctcgaacgc ggtgcccccg
      721 aaccaggcga aacgatggag accgcggccc aggcccagta cgaggtctgg gagatgctcc
      781 gaactatgag gaccgctatg cgccacgacc tcggtgtgtc gcatgaagat tgagagctgg
      841 cactcagctc cgtgcgagct ctcttgtctg cttcgaccag ccaaaaaact gatcgagaca
      901 gaggttaaaa cgcaagctta tcgaggggag gttccggtca gggcgtaccc gggcgcgggc
      961 ttccctcggg agcgcttccg gcggcgtgcc acgccgcggc gaaccggcgg accgatgcga
     1021 accgctcccc gggatcggcc cgggtggcac ggtgcagcac cttgagccgg gccgggccgc
     1081 cccgccaagc gcgttcgtcg tcacccgcgt cgaggagcag ccgggcggcg cggccgagta
     1141 cgaagacggt cgtacgggcg tcgatggtcg cgccgcgctc gtactcctcc ggtgccatga
     1201 accgccgtga gcccggcagc cggtccgcct ccaggacgaa ggggcccggg cggtactcgt
     1261 cgaggtcgac caggcgcatg gtgtcgccgg tgaagtcgta cagcagcgcg ccgtcgtaga
     1321 agtcgactgc cacgtgcccc gcttcctcca ccgcgaggtg cgcactcagg atccggtccc
     1381 acgcacgcat gaccagggac agcggcagcg cccggaaccg cgccatggca ctgcccgggg
     1441 cggtacggtc cgcggtgccc ccggtggtgg ggtggtagag aacctcgccg tcgtgccacg
     1501 gcatgaccac tgcccagttg ccgcgcaccg cgatccggtg cacctgcggg acgatcaccg
     1561 ggtggctcac ggcccggtgg aaggcccacg cccggcagag ggagcgccgc gcgccgtcgc
     1621 tggtgccctc cttgacgaac caccgctctc cgccggggag ccggacgccg taggacacgc
     1681 atcccgagtc ctgatcccgg aagctggcga acaccgtgcc gatgcgttcg aggtgtgcct
     1741 cgacggaggg gagttcggtg acgtccagca acgggtgaga agccatgagg ccatcgtttc
     1801 atcgcccggg atgccggccg ggcggggccg ggtcctcgcc gcgcgacgag gacggggaag
     1861 ccggtgacat cgctgcccta ggtgacgcca gcgacgccaa atagtcgtcg aggagctcga
     1921 cctggcgctg gggcgggaac gacgcggggg cgaacagggc ctgcaccacc agcccgagcg
     1981 cgaaggactg ggcgccggcc gcgatgagct ccgggtcccc ggcgggcagt tcgccgagct
     2041 cctgggccgt ggcgaccagg tcgcggagct tgccgcggct ctgcgcgtac ctgcgggcgt
     2101 ggtcgccgct cagggcgggg tcggcgaggg cgacgtccca ggaggacacc cagatccggt
     2161 tggcgtcggt ggcctcggcg gtcagcggca ggatgtcgag cagggcggcc ctcacagcgg
     2221 gcaggcccgg cccggcagcg cgccgggggc gcgaggcgct gcgctgttcg agcacgtcga
     2281 gggcgtgcgc gaccaggtcg cgcttggcgg ggaagtagtg ggtgagcagg ccggtggtgg
     2341 cgccgagttc ggtggcgacg gcgcgcaggg tcagtccgcc gaagccgtgc gcggccagca
     2401 cccgccagac cgcttcggaa acgtcgcggc ggcgggcttc gtgatctccc ttggtgcgtg
     2461 gcatgctgct acggtacata gtcataacac ttgttatgcg aatggggtca tcatgttctc
     2521 actcttcctg cgggacaacg cctgcctgga gccgctggag atatggcacg ccgaggagtt
     2581 cgccgcccac cttgaccggg cccgcgagca catccggccg tgggtcggac aggcgttcgt
     2641 caccgatgat ctcgacggag cccgggccac gctgcgccgg tacgcagagc ggcaggccga
     2701 ggacggggcc cgcctgtacg gcatccggct cgacggcgtg ctcgtcggcg gcgtgatgtt
     2761 cacggacttc agcaccgccg cgggttcctg cgagatcggt tgctggctgg agcccgccgc
     2821 cgagggccac ggtctggtca ccgacgcgtg cagcgccttg ctggactggg cgttcacctc
     2881 ccgggggctg caccgcgccg agtggcactg ccgggccgac aacgaccgga gctcggcggt
     2941 ggccgtgcgg ctcggcatgg cgctcgaggg cgtgcggcgc cagtcctggc cctacggagg
     3001 cgtccgccac gacaagcaga tctgggcgat cctcgccccc gaatggctcc ggaccggcag
     3061 ccgcgcggcg tagccccgct tcaccaccgt cgtccggctg ctcctcgtac ccgccgtgat
     3121 gaccctcctc ggccgccgcg cctggtggac gccgcgatgg ctggaccggg tgcttcccca
     3181 tctggacgtc gagagcgacg acacgccgcg ccccgcgccc cgcgcgccgc agcgcacggg
     3241 gcacacgggg gtgacagctc tcagcgccgg ccggtggcgg agacctgcgc gccgagccag
     3301 gcccggatcg cgtcggccgt cgtgcgtaca tgctcctcga agagggtcca gtggtcgccg
     3361 ggaacctcca cggcggtgtg cggcgccggc cagaccggcc gcccggggcc gtactcccgt
     3421 gtcccgtccc cggtgccggg acgcaccggc gggagcggct ccgcggcgcg gatcaacagc
     3481 gtggggacgt ccaacggttc cgggtgccag ccgcgggcca tgcgggtgta ggcgcccagg
     3541 gcggccatcg acatgtcgtc cacggccgtg tcgaaccgct cacccagctg gagggggaga
     3601 cgggcgggca tcgcgagcag ccacggctcc tcctcgcggt ccggggtgac gtggtaagtg
     3661 tcggcgatga cgaggccgac cggcggcgtg tccgtcgccg cgagccgcgc ggtgaccgag
     3721 tgggcgggat agccgcccat cgagtagccg aggacggcgt acggacggtc gcccaggtgc
     3781 gtgcgcacgg tgtcggcgtg caggtccacg agggtgcgcc agtcgcgcgg gacggtgtcg
     3841 ccgtgggcgg cgccggggtg gcggaactcg aagacgtccc gctcgccggc gaaggcggcg
     3901 gccagcgcgg cgtaccaggg cctgccgagg ttcggggaga agccggggaa gcacacgagc
     3961 gcgggacggc cggttccttg gacgtcctcg gccagcttga ccggggcgag ctcgtgccgg
     4021 gagctgtcgg cagggccgaa ggagggcagg gcgagcgagg cggtcaccag caggtgcatc
     4081 gccgagacga cgtcgcccgt tccgcacacc cgccggtaga gggaggcgag ggtctgcggc
     4141 ggtcgcgccc ttccgtgcac gtccggtccg gcggcggcag cggtccctgc gagcaggtcg
     4201 ccgcccagct cctcgtacag atgaccggcc agctcggcgg cgctgggccg gtcgaacgtg
     4261 acggtggccg gcagcgtcag gccggtgagc acggcgaggc ggttgcgcag cagcaccccg
     4321 gtcagagagt cgaagcccag ctcggtgaag gggcggccgg gggccacggc agcggcgtcc
     4381 ggatgcccga gcaccgctgc gacctccgcg cggacgagct ccagcagcgc aagcgcccgg
     4441 tcggcttccg gcagcgctgc gagccgttcc cgccaggatc cggtcgcgtc ctcggggccg
     4501 ttgccggctg ccgccttgga gccggtggcg cccacggagc cggcgtcgcc cacggacccg
     4561 gcggccgggc gggccgggcg ggccgggcgc aacaggtcgc gcaggggcgg cgggacgggt
     4621 gatccggcgg gggccgccgg ccgggaacgg tccggcagca gcggggcgag caccggcccg
     4681 ccttcgccca gcgcggcgtc gaacatcgcc aggccctgcg cgggggagac ggcgcgcagc
     4741 acgccgccca tggcccgctc ggcaccgtcg gtccgcgccg ccatcccttc cccgcccgcc
     4801 cacgggcccc acacgaggga aagagcgggc aggccgaggg aagtgcggtg gtgggcgagt
     4861 gcgtccagga aggcgttcgc ggcggcgtag ttggcctggc cgggattgcc cagcagcccg
     4921 gccgccgagg agaacaggac gaagtgtgac aagggcagat gccgcgtcag ctcgtgcagg
     4981 tgccaggccg cgtccgcctt cggccgtaac gctgctgcga accgctcggg cgtcagagcc
     5041 tccaacacgc cgtcgtccag caccccggcg gcgtgtacga cggcgctcag ctgtggcgcg
     5101 tacttctcca cgacggcagc cagctgggcg cgctcggcca cgtcgcacgc gatcacgtcc
     5161 gcgcgggcgc cggcttcctc cagtacggcc tgcagctgcc gcgcccccgc cgactgcggc
     5221 ccccggcggc tgacgagcac cagatgccgg accccgtacc gggtgaccag gtgccgtgcc
     5281 agcaacccgc cgagggctcc ggtgccaccg gtgaccagga cggtgccgac tccgctcgcg
     5341 aacccgtcgg ccgtggggga gtgggtgagt gacaaggttt cggcttctgc cagtcgggga
     5401 acgcgggccg ttccgtcgac cagggagagc tgtggttcgc cggtggcgac ggcggcaggc
     5461 agcaggcgca gcgactccgg actgccgtcg gtatccacga gcgtgaggcg cccggggtac
     5521 tccgcctgcg cggagcgcac caggccccac acggcggcgg ccgacaggtc cggcacgggg
     5581 gcagtggcgc cccgggtcac caggacgaga cgcgttccgg gcgccgccgg aacggacagg
     5641 tacgtctgca gcagtgtcag ggcgcgggcg gtcagacggt gcgtcgccga gacaggatcc
     5701 gttcccgagg acggttcact ccctgaggac ggttcactct ctgaggatgg ttcgctccgt
     5761 gaggacggtt cgctccctga ggacagcgag accacgaccg cctcgggtgc cttcccttgc
     5821 agggcgggcg tcgcagggag cacggaccgc agccccaggt cgtcgggcgc cgccaccgac
     5881 aaggagagct cttcgccgcc gtccggaacg ccggtccacc gcagggtgta caaccctccg
     5941 cggccgttca tgcccacggc cgcggcgtcg acggactccg tcagcggccg ggtggtcaag
     6001 gactccaccc tggccaccgg ctgcccggcg gggtcggcca ggtccacggc gacggtgtcc
     6061 gtaccggtgg cgcgtatgcg tacgcgcagg gccgtggcgc cgacagcgtg cacggtcagg
     6121 ccgcgccagg cgaagggcag ccgggcgggg ccggcggacg gcgcggcgag gagcgccgcg
     6181 tgcagcgctg cgtccagcag agcggggtgg atgccgtacc gccgtgcttc ggcgcccgcc
     6241 gcctcgggca gccgcacctc ggcatacacc tcctccacct cctcgtccgg cccccgccag
     6301 acggccgtga cgccctggaa cgcgaggccg tacgcgtgac cggcgtcggc caggcgctcg
     6361 taggcacccg tgaggtccac gggcacggcg cccgggggcg gccatgcggt ggtggccccc
     6421 cggcgttcgt ccccggctcc ggcggccggg ccgagccgcc cggtcgcatg atgtgtccag
     6481 ccgcccaacg gccctgactc ctgcgggcgg gagtggacgt cggcggtgcg gcgcccggat
     6541 gcgtccggct cgccgaggac cacctgtacc tggacccgct cgccggagcc cggcaggagc
     6601 agcggtgcca gcagcgcgaa gtcctcggcg gtctccagcc cgcacgcgtc gcctgcgtgg
     6661 agcaccatct cggcgaacgc ggcggcaggg acgaggacat gcccggcgac gacgtggtcg
     6721 cgcagccacg gctgggccac ggtggacagg tgaccggtgc acaggaggcg ttccgtgccc
     6781 gggacggtgg tcgtctgagg cagcaacgga tggccggatc cagccgtggc aggagcagga
     6841 gcaggagcag gagcaggaac aggggagggg gaggcagggg cgtccagcca gtagcggcgc
     6901 cgctggaagg ggtacgtcgg caggtccacc cgccgggcgc cgctgccctc gtacactcgg
     6961 tgccagtcca ccggcaggcc gtgtacgtgc agccgaccga gcgcggcaag gaccgcctcc
     7021 gcctcccggg caccgtcccg tgcggtggcg atgaacaccg gcccctcgcc gggcccttcg
     7081 accggccctt cggtcgaccc ctcgtccggc ttcttgccgt cgacggcgag gccggacatg
     7141 gcggcagcgg tgagctgtgc tccgggcccg acttcggcga agacagtcac ccgctcgtcg
     7201 tgtacgagac ggtgtacggc gtctccgaaa cgcacggtct cgcgggcgtg gcgcacccag
     7261 tacgcggccg agaggtcgtc cccggcggcc gggcgcccgg tgagcgtcga catcaccggg
     7321 atgcgtggcg cgcggtacgt cagccgcgac gcgacgtggc cgaacgccgt cagcatcggc
     7381 tgcatcaggg gggagtggaa ggcgtggccg acctgcagcc gtgtggtgcg gcggccccgc
     7441 ccggcgaagc gggccgccac tgtcaggacg gccccctctt cgccggagag gacgaccgac
     7501 cgcgggccgt tcaccgccgc caccgccacg cggtccgccc agccggtcag ctcctgctcc
     7561 gcctcgcgct cggtggcctc cagcgccacc atggcaccgc tgccgggaag ctgccgcatc
     7621 agccgccccc gggccgcgac cagctccacg gcgtccggct ccgacaggac tcctgcgaca
     7681 tgagcgaccg ccagttcacc gacggagtgc ccggacagga cgtccggccg tactccccag
     7741 gactccagaa gccggaagag cgccacctcg aagacgaaca gcccggcctg cgcgaagtcg
     7801 gtccgtgccg acagcgtggc gtcgtcgctg cctgcctccg cccacagcac ggtgcgcagg
     7861 ggccgctcca ggccgccctc ggcatcgaac cgttcacaca aggagtcgaa ggcggcggcg
     7921 aacgcaggga aggcgccgtg cagttcccgg cccatgcccg ggcgctgcgc accctggccg
     7981 gagaacagca gggccagccg accggaggat gtggccggcc tggccgtggg gctgctgcca
     8041 ccgtttccgc cgttgctgcc gctgccgctg ccgctgccgc tgccgctgcc gctgccggtt
     8101 ccggcagccg cctgtgcgtc cagagcgcgc agcagtgctg tgtggtccgc gccggtcacc
     8161 gccgcccggt gagcgagcgc ggtccgggtc gtggccaggg agaacgcgac gtcgagcagg
     8221 ggtggccggg gatgctgccg cagcagggcc gccacctgcc cggcctgcgc gcggagagct
     8281 cgcgggtccg cgccggacag cagcaggggc ggcacggcct gcgggcgccc gggagccgtg
     8341 ccggcatcgc cttccgctcc gggttccgtc tcccagggag cttcctccag gatgacgtgg
     8401 gcgttcgtcc cgccgatgcc gaacgccgac acccctgccc ggcgcgggct gccgggcgcc
     8461 ggatgccatt cctcctcgcg cgtcagcagc cgtacggtgc ccgacgacca gtccacgcgg
     8521 ggggacggtt cctcgcagtg caggatgcgc ggcaggcgtc cgtggcgcat cgcaaggacg
     8581 gtcttgatga cgccgccgac accggccgcg gcctgggtgt gcccgatgtt ggacttcagc
     8641 gcaccgagcc acagcggccg gccggcagac cgctcctgcc cgtatgcggc cagcagcgct
     8701 cgggcctcga cggggtcgcc gagtgcggta ccggtgccgt gtgtctcgac ggtgtcgatg
     8761 tcggccgggg acagaccggc gtcggccagg gcctgacgga ccacctgctg ctgggccgtg
     8821 ccgctgggtg cggtgagacc gttggaggca ccgtcggagc cgacggccga gccgcggagc
     8881 acggccagga ccggatggcc gttgcgccgg gcgtcggaca ggcgctccag caggaccact
     8941 ccagcgccct cggcccagcc ggtgccgtcg gccgcggccg agaacggttt gcaccggccg
     9001 tccggcgcca ggccgcggag cctgctgaac gcgacgaagg gcgccggggt ggccatcacg
     9061 gtgacgccgc cggccaccgc cagcgagcac tcgcccgacc gcagcgcctg cgccgcccag
     9121 tgcatcgcca ccagggacga cgagcaggcg gtgtccaccg tgacggccgg cccgtgcagg
     9181 cccagggtgt acgcgatacg gcccgacgcc acgctgcccg tcgagcccag cgcgagatgc
     9241 gcttcgagcg ggtgcgcgcc cccgtgcagg agacggcctg cgtagtcgtc gtgcatcacg
     9301 ccgacgaaga cgccggtgtc gctgccgcgg aggtccgccg ggtcgatacg cgcccgctcg
     9361 cagagctccc aggtggtctc cagcagaagt cgttgctggg gatcggtcgc cagtgcctcc
     9421 cgcggggaca ttccgaagaa ggccgcgtcg aagtcggccg ccaggcgcag gaagccgccg
     9481 cggcgggtga cggaggcgcc gagccggtcc gggtcggggt cgaagaggtg caccgggtcc
     9541 cagccgcggt cgccggggaa gtccgaggtc gcgtcagccc cgtccgcgac cagctgccac
     9601 agttcctccg gagacgtgac gtcgtcacgg agtcccggat accggcatgc catcgagacg
     9661 atggccaccg gatccccgga cgctgtacgg gacttggcgc ccgccgtacg ggacttggcg
     9721 cccggcgtac gggctttggc gccgcgtgtc cgggctgccg gtgcacggcc ggggaggagc
     9781 gcggagtgca ggaactccgc cactgcggca ggcgtcgggt ggtcgaacac cagggtcgcg
     9841 ggcagctcca gcccggtgtc cgcgccgagc cggtcgcgca gcagtacgcc cccgagggag
     9901 gtgatgccgg aggcggtgaa gggccgcccc ggctccggct ccgtctcctg ctccggcgcg
     9961 ccaccggcgt ccgccgtacg ggcgaggacg aggcgcagca cggcgtcgta ccgtccggac
    10021 gggggcaggg acagcaggcg ccggcgcagg gcggatacgg cgtcctcacg caccgctccg
    10081 agccgttccg ccaactgccg ggccagcgcg ggacggtcgg tcttgccgga gggggtacgg
    10141 ggcacggagc cggtctcgta cacctcgtcg ggcaccttga agcccgacag ccgctcccgg
    10201 cacgcggcgc gtacccggtc ggtgtcgacg ccgtgcgggc cggggacgac gaaggcgacc
    10261 gggacctcgc ccagcacgtc gtgcggcctg ccgacgacgg ccgcgtccgc gacaccgggg
    10321 cagccgagca gcacatgctc gacctcgacc gggttgacgt tctctccacc gcggatgatc
    10381 agttccttga cccggccggt gacctgcaga cgcccgccgt caccgcggcg cccgagatcc
    10441 ccggtgcggt accacccgtc cctgaaggcc tgtgcggtgt cgtccgggcg gttgtgatag
    10501 ccgagcatca ggttcgggcc gcggacccag atctccccgt ccgtaccatc ggccacgtcg
    10561 tcaccaccgg caggatcggt cagccgcacc tcgactccgg gcaccggcag aacgccggag
    10621 cggccctccg gagggccgcc gggccggtcg accgcgatct tcccgcaggt ctcggtgctg
    10681 ccgtagccgt cgagcaacgg ggcacccagc agctcctcga cctccgctcg cagcgccggg
    10741 ggacacggcg cgcccgcggt gacgcacagc cgtacgcccg acggccgccg cggtcgcgac
    10801 gcctcgcgca gcgcctcagt gagcaggtgg tacgtggcgg gcactccggc cacgaccgtg
    10861 cacggctgct cggcgagcgc ctgcgcgacg ctcgcgcccc ggccgaggag gtaggcactc
    10921 gcccccacgg agatgacgcc cagcaggcac agcgagtggg cgtaggcatg ggacatcggc
    10981 agcggccaca gcagccggtc ctgccgctcc aggccccaca gcggcacgta ggcggccgcc
    11041 accgaccaca gtgcggtgcg ctgactggag acgacgccct tgctccgacc ggtggtgccg
    11101 gaggtgtaga ggatccaggc cggttcgtcc aggccgaggt cgtcccgagg cgcctgcccc
    11161 ggatccgtcc cggccagctg ctcgaagagg tgctcggctg cgggcgcccc gtccggcacg
    11221 gcgtccgtac ccgccaccac gacccgtgcg tgcagccgcg gccgcgtggc cgccagtccg
    11281 gcgacccggg acagccggct ctcctcggtg atcaccgcga cggcgccgct gtcgtcgagg
    11341 aaatacgcca gctccgcctc cgtcgccccg ggatcgagcg gaacgcctac ggcccccgcc
    11401 cgcaggaccg cgaggaaccc ctccaccatc tccacccggc tgcccaggtg gagggccacc
    11461 cggtcgcctc gccgcacccc cagccgtgcc aggtgacccg ccaggcgccc ggtgcgcttc
    11521 tccagctcac cgtaagtgac accccgctgc gcatcccgga aagcgatctt gcccgctatc
    11581 tgcatggaat gtgccttgag cacgccgggc agtgcacgca tcaactcgcc atggatctcc
    11641 gtcatccccc acccctccgg acacggggcg aaggagagcg ctcccgctcg cacaccgtac
    11701 cgagtagggc tgacgccaca gctcggagcg agcggcccag gctcccgggc ccccaggtcc
    11761 ccgggcccca ggcccccggg tggaccacgg gccgcgccgg ggccggatga tctgcatatc
    11821 gtggccccat gggtgaagag agcggcgccg ccggcacgcc ccacggcccc gccgccccca
    11881 ccggaaggcg gcatcgcgcc cgccaccgct ggtcggcgtt ccgtcactcg ccgttcttcc
    11941 ccgccgctgt gctcgtgctc atcgtggccg cggccgccgg gctcttcgcc ggctcctaca
    12001 cctacgccat ggccaatccg accccgcaca acatccccac cgccgtcacg ggcgtcccgg
    12061 agtccgagat caagcgggcg gaattcgtcc agggcatgga gagagcgctg ggcgccgagc
    12121 tcctgctgcg ccacttcgcc acctacgagg acgcccggcg tgcggtcgag gaccagcgcg
    12181 tctacgccat cctgcaggac cgccaccacg cggccgagct ggacgtctcc ggagcatccg
    12241 gcgccaccgt ggcccagctg ctgaccgaga ccgcgccccg ggtcggcaag gaaatcagcg
    12301 tcctcgtcgt cgtgcgggac gtcaatcccc tccagaaggg cgatccgcgc ggcctcgcgc
    12361 tcttctacat caccctggcg gccgtcatcg tcggcttcgt cggcgccatt cagatgagcg
    12421 tccacgcggg cgaactcaat ccggccgaac gcatcgcctt caccgccgcc tattccacgc
    12481 tgggcggctt caccatcgcc gcggtcgtgg actgggggct gggtgcggtg cggctgccgt
    12541 tcgcggaatc ctggctgatc ctggcgctga ccatgttcac ctcgggcatg gtcttcacga
    12601 tgttcaacgc cctcttcggg cggtgggcga tgctgcccac ctggggcctg atggtcctgt
    12661 tgggcaaccc ctcctccggc ggtgccgtct cctggccgct gctgccctcc ctcctcggcg
    12721 agatcggccg ctggctgccc ccgggggcct ccgtgaacgc ccagcacacg gccgtctact
    12781 tccggcagca ccagcacttc ttcccattcg ccgtgctgat cgcctgggcc gccctgtcct
    12841 gcgcgatctt ctggacctgg cggcaccgcc accccggcgg acgcgacacg gccgcggccg
    12901 cggccgtgtc cgcgggctga accttcccca atcggcgctg acgctgtgcg catgtgcgct
    12961 cgctgtgtgt cgtctcgggg gctgttccgt gtgagggcgc ccatgaggcc tgggtcactt
    13021 acgaatgccg ttagtggaat tcggtggcgg aa
//
