LOCUS       NZ_BMUT01000027        14768 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces hiroshimensis strain JCM 4586 sequence27, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUT01000027
VERSION     NZ_BMUT01000027.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces hiroshimensis
  ORGANISM  Streptomyces hiroshimensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 29763)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 90X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650335.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:32:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,830
            CDSs (total)                      :: 6,745
            Genes (coding)                    :: 6,620
            CDSs (with protein)               :: 6,620
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 25 of 125
            Pseudo Genes (incomplete)         :: 107 of 125
            Pseudo Genes (internal stop)      :: 4 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:07:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 14768
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUT01000027.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..14768
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RRE-containing"
                     /region_number="1"
                     /rules="minimum(1, [Bottromycin_Methyltransferase_RRE,
                     Cyanobactin_D_RRE, Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /tool="antismash"
     cand_cluster    1..14768
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /kind="single"
                     /product="RRE-containing"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..14768
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[4537:4768](-)"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(4538..4768)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="minimum(1,
                     [Bottromycin_Methyltransferase_RRE, Cyanobactin_D_RRE,
                     Cytolysin_C_RRE, Goadsporin_CD_RRE,
                     Heterocycloanthracin_C_RRE, Lanthipeptide_LanB_RRE,
                     Lasso_Fused_RRE, Mycofactocin_RRE, NHLP_CD_RRE,
                     Other_Sactipeptide_rSAM_RRE, Pantocin_Microcin_RRE,
                     Plantazolicin_C_RRE, PlpY_RRE, PqqD_RRE,
                     Proteusin_Epimerase_RRE, Proteusin_Methyltransferase_RRE,
                     Quinohemoprotein_rSAM_RRE, Ranthipeptide_rSAM_RRE,
                     Stand_Alone_Lasso_RRE, Streptide_RRE, Subtilosin_rSAM_RRE,
                     Thiaglutamate_B_RRE, Thiaglutamate_G_RRE,
                     Thiaglutamate_I_RRE, Thiopeptide_F_RRE, Thuricin_rSAM_RRE,
                     Thurincin_rSAM_RRE, Trifolitoxin_RRE])"
                     /neighbourhood="10000"
                     /product="RRE-containing"
                     /protocluster_number="1"
     gene            complement(217..2109)
                     /locus_tag="IE241_RS33440"
                     /old_locus_tag="GCM10010324_68110"
     CDS             complement(217..2109)
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="resistance (resist) ABC_efflux"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018491384.1"
                     /locus_tag="IE241_RS33440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68110"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_190025639.1"
                     /transl_table=11
                     /translation="MSMEVTAWTSLHSAMNAEKSRRPFSRVTLRRIGGFARPHRRKIGQ
                     FLLLSVAMALLAVATPILAGRVVGAIVHGDDAGTVTRLALLIALIAVAEAGLGLLTRWL
                     SATLGEGLILDLRTAVFDHVQRMPVAFFTRTRTGALVSRLNNDVIGAQRAFSNTLSGVV
                     SNIVTLLLTLGVMLSISWQITLLALVLLPVFVVPARRMGSRMAGLQREAAAHNAAMSNQ
                     MTERFSAPGATLVKLFGRPAEESAEFASRARAVRDIGVRTAMAQSAFITALTLVSALAL
                     ALVYGLGGFYALHGRLEPGAVVALALLLTRLYAPLTALAGARVEVMSALVSFERVFEVL
                     DLKPLIAEKPGARRVPEGPVSVEFDDVRFGYPAADKVSLASLEDVAGLDTRGGAEVLHG
                     VSFRAEPGRMVALVGSSGAGKSTIAQLLPRLYDADAGSVRLGGVDVRDLSAASIRETLG
                     MVTQDGHLFHDTVRANLLLARPEATEEEVWDALRRARLDGLVASLPDGLDTVVGERGYR
                     LSGGERQRLTIARLLLARQRVVILDEATAHLDSTSEAAVQEALAEALEGRTAVVIAHRL
                     STVRAADLILVVEDGRVVERGTHSELIAAGGRYEELHRTQFARAKADGEAEPVC"
     gene            complement(2251..4089)
                     /locus_tag="IE241_RS33445"
                     /old_locus_tag="GCM10010324_68120"
     CDS             complement(2251..4089)
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003955660.1"
                     /locus_tag="IE241_RS33445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68120"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_190025640.1"
                     /transl_table=11
                     /translation="MTAPPSVTSPGTGPAAPPGAGAPEDRGRSWPGQRALLGHLRPQRR
                     RMAAGVLLGLVGSVMGLAQPLAAKRVVDDMGRGAPAAGPVLVLSLLVVAGAVLAGLGYY
                     VLQYSAESIVCTARRRLTDRLLRLRVPVVESSEPGDLIARVTSDTTLLRQTAPQAVSAA
                     VTGTLVTAATVVMMGLLDAVLLTVTLAVVAFVGVVVALVAPRIGRATRHTQEAVGCIGA
                     ALERALGAFRTVKASGAEQREAEALCRAAERARRTGVRAAAWEAVSATTSAVVVQVCFL
                     VVLGVGGARVTSGATDVSTLVAFLLYLFALAPRIGQLVEGVGQVQVGSAAADRIAQVEQ
                     LETEKPGTEMPTREESQTAEQEDEGVRSPLSVSFDQVSFAYRPGLPRVHHGVSFTVPAG
                     GLTAFVGPSGAGKTTVFSLIERFYEQDSGRVLLDGEDVRQWPLARLRASIGYVEQDVPV
                     LAGTLRDNLTLGTSDPDPGELARVLRLARLDVLADRLPDGLDTDVGHRGSRLSGGERQR
                     VAIARALLRRPGLLLLDEVTSQLDAANEAALRDTVTDTARHTTVLVVAHRLSTVTTADR
                     IVVMEAGRVRAVGTHAELLAHDDLYRDLASTQLLGP"
     gene            complement(4094..4510)
                     /locus_tag="IE241_RS33450"
                     /old_locus_tag="GCM10010324_68130"
     CDS             complement(4094..4510)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF13471"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047207.1"
                     /locus_tag="IE241_RS33450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68130"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_190025641.1"
                     /sec_met_domain="PF13471 (E-value: 1.2e-27, bitscore: 87.0,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTPEVIPYHPRSIPLARRCAARLAVGCAHLLATQPPARIRAVLC
                     RLRRGARPATLAEASTARRTVLAVSLAAGGHKGCLPRSLATVLLCRWYGQWPTWCVGVR
                     SRPPFAAHAWVEAEGVLVGEGAPADYFQRFFTVE"
     gene            complement(4538..4768)
                     /locus_tag="IE241_RS33455"
                     /old_locus_tag="GCM10010324_68140"
     CDS             complement(4538..4768)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RRE-containing: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986678.1"
                     /locus_tag="IE241_RS33455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68140"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_229900214.1"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 3.9e-20,
                     bitscore: 62.6, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 2.8e-15, bitscore: 47.3,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTETEYGVALLDQESAEYWTLNPTAALVLQTLLDGHGPERAVEML
                     TARYDDVDAGLAGEDVRRIIGELSAAGLLTP"
     aSDomain        complement(4544..4765)
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IE241_RS33455_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="4.20E-20"
                     /identifier="RREFam002.1"
                     /label="IE241_RS33455"
                     /locus_tag="IE241_RS33455"
                     /protein_end="75"
                     /protein_start="1"
                     /score="62.6"
                     /tool="antismash"
                     /translation="TETEYGVALLDQESAEYWTLNPTAALVLQTLLDGHGPERAVEMLT
                     ARYDDVDAGLAGEDVRRIIGELSAAGLLT"
     gene            complement(4800..6661)
                     /locus_tag="IE241_RS33460"
                     /old_locus_tag="GCM10010324_68150"
                     /pseudo=""
     CDS             complement(4800..6661)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986679.1"
                     /locus_tag="IE241_RS33460"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68150"
                     /product="lasso peptide isopeptide bond-forming cyclase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MDQSLSLQSVLSWFVVLPDSDAAAAPAAKALSFASRSLLHASGRP
                     WIVGRWTPGTVVTGTHRDTRVAVMGSTPSRHSTPNGPPPRRPAPLRRPRWTASQGGPAA
                     ST"
     gene            complement(6738..6845)
                     /locus_tag="IE241_RS33465"
     CDS             complement(6738..6845)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039305.4"
                     /locus_tag="IE241_RS33465"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="keywimysin-related RiPP"
                     /protein_id="WP_190025643.1"
                     /transl_table=11
                     /translation="MTYERPTLAKAGSFRKVTGLKDHGPKDVLGGRQVL"
     misc_feature    complement(6867..6882)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     misc_feature    complement(7071..7086)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.87"
                     /tool="antismash"
     gene            complement(7113..8522)
                     /locus_tag="IE241_RS33470"
                     /old_locus_tag="GCM10010324_68170"
     CDS             complement(7113..8522)
                     /GO_function="GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aldedh"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1017: aldehyde dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004940859.1"
                     /locus_tag="IE241_RS33470"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68170"
                     /product="NADP-dependent succinic semialdehyde
                     dehydrogenase"
                     /protein_id="WP_190025644.1"
                     /sec_met_domain="Aldedh (E-value: 2.1e-152, bitscore:
                     498.6, seeds: 89, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPIATVNPANGETLRTFEEMGAEEIEQCLAAAAAAFRRYRTSDPG
                     ERARLLNRAADLLEEDRDDIARTMTTEMGKPLAAARAEAAKCVKAMRWYAANAAELLAD
                     EHPPQPEVADSGAALARIRYRPLGVVLAVMPWNFPLWQVIRFAAPALMAGNVGLLKHAS
                     NVPQTALYLGDLFRRAGFPDGCFQTLLVGSRAVEGILRDPRVAAATLTGSEPAGRSVAS
                     IAGDEVKKTVLELGGSDPYIVMPSADLDRAVQTAVTARVQNNGQSCIAAKRFIVHADVH
                     DAFLERFAARMAALTVGDPMEEATDVGPLASEQGRADLEELVDDAVRRGATAVCGGRRP
                     KERPKGWFYEPTVLTGITPDMRIHREEAFGPVATVYRVDGVDEAVELANDTPFGLSSNV
                     WTTDAEEQERFARDIEAGGVFFNGMTASHPALPFGGVKRSGYGRELSGHGIKEFCNVTT
                     VWHGVEPAREA"
     gene            complement(8710..10044)
                     /locus_tag="IE241_RS33475"
                     /old_locus_tag="GCM10010324_68180"
     CDS             complement(8710..10044)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1202: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006607000.1"
                     /locus_tag="IE241_RS33475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68180"
                     /product="MFS transporter"
                     /protein_id="WP_190025645.1"
                     /transl_table=11
                     /translation="MRTLRAVRECPLALRLLFINQCGIDAGFYLLIPFLATYIGHDLGM
                     SAAIVGLVLGVRNLSQQGMYIIGGTAADRLGPRVVIIAGCALRTVGFTLFSFGNSLPLL
                     LAASGISGLAAALFYPAVRSYVSHESGERKAEAFALLNVFATIGSLVGLLAGSVLYLTD
                     FRICALTAAGLFAALTVAQAFVLPAREVAAEHDPILRELREVLGNRRFLCFAFATTGMF
                     IMENQLYMLLPDGAQQASGWGGAEALLLSAGAVANLLFQFRITRGLEQRGGGMRWVGAG
                     IAVIGLGFVPPLLVCDAAPPDGAGDVVPRLLVMVSASLLLYFGGMVAYPPVLGLIPRFG
                     SERLTGTYFGLFYVLSGATAAGGNAVIGWVMDVGERTGRAWLPWVSCLFFGLASGCAVA
                     WLYRSRALPCPYPAESGRGAGTQEAMGLLPGQPRVTDDAGERRTG"
     misc_feature    complement(10393..10404)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.42"
                     /tool="antismash"
     misc_feature    complement(10557..10575)
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.12"
                     /tool="antismash"
     misc_feature    10589..10603
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.12"
                     /tool="antismash"
     gene            10663..11052
                     /locus_tag="IE241_RS33480"
                     /old_locus_tag="GCM10010324_68190"
     CDS             10663..11052
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174704.1"
                     /locus_tag="IE241_RS33480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68190"
                     /product="hypothetical protein"
                     /protein_id="WP_190025646.1"
                     /transl_table=11
                     /translation="MNDRNFLERTKQFALYLAELQTSLDPESYALLLRILKGFNEAIVH
                     RERGVDIPFSPREKELFTPEFGRVLTRLAGFLGPQGMHVVVGTHGAPADGGPPGGAYPG
                     DHGTDPGDHDRREIDAIARASGMRP"
     gene            11131..11859
                     /locus_tag="IE241_RS33485"
                     /old_locus_tag="GCM10010324_68200"
     CDS             11131..11859
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1188: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539230.1"
                     /locus_tag="IE241_RS33485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68200"
                     /product="DedA family protein"
                     /protein_id="WP_190025647.1"
                     /transl_table=11
                     /translation="MGGHALVPASPAADLTTTLAVNLLDASSLLTAFGALGIAVVMFAE
                     TGLLIGFFLPGDSLLFTAGLLARSGAHSGPHLDLAQVLAASAAGALTGAQVGYVLGRRG
                     GRALLTRSRSRKLHEGAERAEELLGRYGHARAIVLARFVPVVRTVLNPLAGALGVAPRT
                     FTLWQVTGGVVWTVGLVLAGYGLGSAVPDVDRYLLPIVGAVVVLSLLPVALELLRSRGR
                     RRAVTPETSPAPAPNARQEP"
     gene            complement(11807..12706)
                     /gene="cynR"
                     /locus_tag="IE241_RS33490"
                     /old_locus_tag="GCM10010324_68210"
     CDS             complement(11807..12706)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene="cynR"
                     /gene_functions="regulatory (smcogs) SMCOG1014: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539477.1"
                     /locus_tag="IE241_RS33490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68210"
                     /product="transcriptional regulator CynR"
                     /protein_id="WP_190025648.1"
                     /transl_table=11
                     /translation="MELRHLRYLLAVAEHANFTRAAEALHISQPTLSQQIKQLERSLRV
                     QLLDRSGRTVRLTDAGEVYTRYARHALQDLAAGERAVLDVQDLSRGSLRLAVTPTFTAY
                     LIGPLVAEFHARHAGIALDLREMAQDRIEAALLADELDLGIAFSGAHLPGVAGTGLFTE
                     TLGLVVGPDHPCAGRSEPLPVGELGDHPLALLSRDFATRGHIDAFLAEHGVTPRIAVEA
                     DSINALTEIVGRSALATVLPEAITGGPWQLHPVPVAPPLPARTVTLLCREAGYRSAAAR
                     AFTALAGRWVRERGWSPA"
     gene            complement(12802..13281)
                     /locus_tag="IE241_RS33495"
                     /old_locus_tag="GCM10010324_68220"
     CDS             complement(12802..13281)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006607308.1"
                     /locus_tag="IE241_RS33495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68220"
                     /product="NUDIX domain-containing protein"
                     /protein_id="WP_190025649.1"
                     /transl_table=11
                     /translation="MPTPDFIRDLRATAGRQLLWIPGVSAVVLDDEGRVLLGRRSDNGQ
                     WAIIGGIPDPGEQPAAAIVREVYEETAVRCVAERVLVVEALDPVTYPNGDVCQFMDTCF
                     LCRAVDGEACVNDDESLEVGWFEPDALPPMQEFAVRRIKHATENGATWFAGIGEG"
     gene            complement(13367..13972)
                     /locus_tag="IE241_RS33500"
                     /old_locus_tag="GCM10010324_68230"
     CDS             complement(13367..13972)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006350773.1"
                     /locus_tag="IE241_RS33500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_68230"
                     /product="hypothetical protein"
                     /protein_id="WP_190025650.1"
                     /transl_table=11
                     /translation="MRDHSTGPQGHEGDEKTGAAGSPSPADGAEDLAVWTGRATNRVQW
                     LLAAAGAACLALGVELAVDSAWTSGLAPLVMAVVGCVAAGLLMLYGTLAFVHVAVRVDR
                     EALEVRCGHIGVPRTRILLAHVVDADFAPRVTPRQWGGWGYRWRPEKGTAVVVRRGEGF
                     VLRLGDGRTFTVTVDDAESAVRVIRQALRLAPGRKAPA"
ORIGIN
        1 ccctcgccga cgtcgtcgcc gaggacgagc tcaacgcgaa ctacatcatc cccagcgtct
       61 tcaacccgaa ggccacggag tccgtcgcgg cagccgtcaa gcgtgcggcc ctggagctcc
      121 gtcaggccga gtagcagcgc atcccgcctg aacgagcggc ctcccggcgg cggaatgccg
      181 tccgggaggc cgctttttcg gatgtccgtc cgcccgtcag cagaccggct ccgcctcccc
      241 gtcggccttc gcacgcgcga actgcgtgcg gtgcagctcc tcgtagcgtc cgcccgcggc
      301 gatcagctcg ctgtgggtgc cgcgttcgac gacccggccg tcctcgacga cgaggatgag
      361 gtcggccgcc cgcacggtgg acagccggtg ggcgatcacc acggccgtac ggccctccag
      421 ggcctcggcc agcgcctcct gaacggccgc ctcggaggtg gagtcgaggt gggcggtcgc
      481 ctcgtcgagg atgacgacgc gctggcgggc cagcagcagc cgggcgatgg tcagccgctg
      541 gcgctcgccg ccggagagcc ggtagccgcg ctcgccgacg accgtgtcca gaccgtcggg
      601 cagggaggcc accagcccgt cgagccgggc ccgccgcagc gcgtcccaga cctcctcctc
      661 ggtggcttcc ggccgggcga ggaggaggtt ggcgcgcacg gtgtcgtgga agaggtggcc
      721 gtcctgggtg accatgccga gcgtctcgcg gatggatgcg gcgctcaggt cccggacgtc
      781 gacgcctccg aggcgtacgg atccggcgtc ggcgtcgtag agccggggca gcagctgggc
      841 gatggtcgac ttccccgcgc cggaggagcc gaccagggcc accatccggc ccggttcggc
      901 gcggaaggag acgccgtgca ggacctcggc gccgccgcgg gtgtccaggc cggcgacgtc
      961 ctccagggag gcgagggaga ccttgtcggc ggccgggtag ccgaaccgga cgtcgtcgaa
     1021 ctcgaccgag accggcccct ccggcacccg ccgggcgccc ggcttctcgg cgatcagcgg
     1081 cttgaggtcg aggacctcga agacgcgctc gaagctgacg agggcgctca tcacctcgac
     1141 acgggcaccg gcgagggcgg tcagcggggc gtagaggcgg gtgaggagca gggcgagcgc
     1201 gacgacggcc cccggctcca gccggccgtg cagcgcgtag aagccgccga gcccgtagac
     1261 cagggccagc gccagggcgg agacgagcgt gagggcggtg atgaacgccg actgggccat
     1321 ggcggtgcgt acgccgatgt cgcgcacggc gcgggcacgg gaggcgaact cggccgactc
     1381 ctccgcgggc cgcccgaaca gcttgacgag ggtcgcaccg ggcgcggaga accgctcggt
     1441 catctggttg ctcatggcgg cgttgtgagc ggcggcctca cgctgcagcc cggccatgcg
     1501 cgagcccatc ctccgggcgg gcacgacgaa caccggcagc aggacgagcg cgagcagcgt
     1561 gatctgccag gagatgctga gcatcacgcc gagcgtcagg agcagcgtga cgatgttgga
     1621 gacgacgccg gagagggtgt tgctgaaggc gcgctgagcg ccgatgacgt cgttgttgag
     1681 ccggctgacg agggcgccgg tgcgggtgcg ggtgaagaag gcgaccggca tccgctggac
     1741 gtggtcgaag accgcggtcc gcaggtcgag gatgagcccc tcgccgaggg tggcggacag
     1801 ccaccgcgtc agcagcccca ggcccgcctc ggcgacggcg atcagggcga tgagcagcgc
     1861 gagccgggtg acggtgccgg cgtcgtcgcc gtgcacgatg gcaccgacga cccgcccggc
     1921 caggatcggc gtggccaccg cgagcagcgc catggccacg ctcagcagca ggaactgccc
     1981 gatcttcctg cggtgcggcc gcgcgaagcc gccgatgcgc cgcagcgtga cccgggagaa
     2041 gggccgtcgg ctcttctcgg cgttcatcgc gctgtggagc gaggtccacg cggtgacttc
     2101 catgctcatg gggggctcct ccggtgtcgt cgtatgcctg acacgctaga acctcaacca
     2161 aggttgagat caagcgtcgc accggcgggc cgggaaagcc acgagaaccg ggaaagccac
     2221 gagaacaaga aacaccacgg aagcgccggg ctacgggccg agcagctgtg tgctcgccag
     2281 gtcgcggtag agatcgtcgt gcgccagcag ctcggcatgg gtgccgaccg cccggacgcg
     2341 tccggcttcc atgaccacga tccggtccgc tgtggtcacg gtggagagcc ggtgggcgac
     2401 caccaggacg gtggtgtggc gggcggtgtc ggtgacggtg tcgcgcagcg ccgcctcgtt
     2461 ggcggcgtcg agctgggagg tgacttcgtc cagcaggagc aggccggggc ggcggagcag
     2521 ggcgcgggcg atcgccacgc gttgccgttc gccgccggac agacggctgc cgcggtggcc
     2581 gacgtcggtg tccagaccgt ccggcagccg gtccgcgagc acgtccaggc gggccagccg
     2641 caggacacgg gcgagctccc cggggtccgg gtcggaggtg cccagcgtca ggttgtcgcg
     2701 cagagtgccg gcgaggacgg ggacgtcctg ctccacgtag ccgatggacg cgcggagccg
     2761 ggccagcggc cactgccgca cgtcctcgcc gtccaggagc acacggccgg agtcctgctc
     2821 gtagaagcgc tcgatcaggg agaagaccgt ggtcttaccc gctccggacg ggccgacgaa
     2881 cgcggtcagg ccgcccgcgg gcacggtgaa ggagaccccg tggtggacgc gcggcagtcc
     2941 ggggcggtag gcgaagctca cctgatcgaa cgacacgctc aggggcgacc gtacgccctc
     3001 atcctcctgc tctgccgtct gcgactcctc cctcgtcggc atctccgtcc ccggcttctc
     3061 cgtctccagt tgttcgacct gggcgatgcg gtccgcggcc gccgagccca cctggacctg
     3121 gccgacgccc tcgaccagct ggccgatgcg cggggcgagg gcgaagaggt agagcaggaa
     3181 ggcgacgagc gtggagacgt ccgtcgcacc ggaggtgacg cgcgcgcccc ccaccccgag
     3241 taccacgagg aagcagacct ggacgaccac cgccgacgtc gtcgccgaca ccgcctccca
     3301 ggccgcggcc cggaccccgg tgcgccgggc gcgttcggcc gcccggcaca gcgcttccgc
     3361 ctcccgctgc tcggcacccg acgccttgac ggtccggaag gcgcccagcg cccgttccag
     3421 cgccgccccg atgcagccca ccgcctcctg cgtgtggcgg gtcgcgcggc cgatccgcgg
     3481 cgccacgagt gcgacgacga cgccgacgaa ggcgaccacg gcgagggtga cggtcagcag
     3541 gacggcgtcg aggaggccca tcatgacgac cgtcgccgcg gtgaccaggg tgccggtgac
     3601 cgccgccgac accgcctggg gcgcggtctg gcgcagcagc gtcgtatcgg aggtcacgcg
     3661 ggcgatcagg tctccgggct cggagctctc caccacgggc acgcgcagcc gcaggaggcg
     3721 gtccgtcagc cggcggcggg cggtgcagac gatggactcg gccgagtact ggagcacgta
     3781 gtaccccagc cccgcgagga cggccccggc caccaccagc aggctgagca cgaggacggg
     3841 accggccgcg ggcgctcccc ggcccatgtc gtcgaccacg cgcttggcgg ccaggggctg
     3901 ggccaggccc atgacgctgc cgacgagtcc caggaggacg cccgcggcca tgcgccggcg
     3961 ctgcgggcgc agatggccga gcagggcccg ctgccccggc cacgagcgtc cccggtcctc
     4021 cggcgcaccg gcccccgggg gcgctgcggg gccggtgccg ggagaggtta cggagggcgg
     4081 cgcggtcatc ggctcactcc accgtgaaga agcgctggaa gtagtcggcc ggggcgcctt
     4141 cgccgacgag gaccccctcg gcctccaccc aggcgtgcgc ggcgaacggc ggccggctgc
     4201 gcactccgac gcaccaggtg ggccactggc cgtaccaccg gcagagcagc acggtggcca
     4261 gggaccgggg caggcatccc ttgtgccctc cggcggcgag gctcacggcg agcaccgtcc
     4321 gccgcgccgt gctcgcctcg gcgagcgtgg cgggccgggc gccgcgccgc agcctgcaca
     4381 ggacggcgcg tatccgggcg ggcggctggg tcgccagcag gtgggcgcag ccgacggcca
     4441 gccgggccgc acaccggcgg gccaggggga tggagcgggg gtggtagggg atcacttccg
     4501 gtgtcgacat ggcgtgcctc cagatcggca gggcgtccta cggggtgagc agtcctgccg
     4561 cgctcagctc gccgatgatg cggcggacgt cctcgccggc gagcccggcg tcgacgtcgt
     4621 cgtagcgcgc cgtcagcatc tcgaccgccc gctcggggcc gtgtccgtcc agcagtgtct
     4681 gcaggacgag cgcggccgtc gggttgagcg tccagtactc ggcgctctcc tggtcgagca
     4741 gcgccacgcc gtactcggtc tcggtcatca ggacaccagg tttgagtgcg ggcacgggtt
     4801 cacgcctctc ggtcggcggc gggcaggggc gccgtctccc gcgagcgcag ccacgcttcg
     4861 caggcgatgg tcgggtggag cagatagctc tccgtctccg cggagagggc gcggcggcac
     4921 cattcgtgca gcaccttccc gtcggccagg ccgagccgga ccagccggga gtcctcgcac
     4981 agggccagca ccgcccggcg gttgcggcgc agcccggcct cctcctcgta cgtcgcgttg
     5041 gccttggtgc tgcgggtgcg gccggcctcg ggcacgacgc cgcgcatggc ctcgacgatg
     5101 agcggcttgt accgccaggg ggtgatccgc tcctgcgggc gtacggcgag ggccgcctcg
     5161 atgacgcggg tgtcgtagta gggcgcggag atctcgatgc ccaggggggc ggccatctgc
     5221 ccgatgtgcc ggacccactg ggagatgccg tccatggcga ccagttcgcg gtgttcgccg
     5281 cggttccggc cccggggtcc ggcggggttc cggcgggtga ggagcagttc gcgtgccgcg
     5341 cggaccgcgt cgggcgtggc ccacggcggc atccggggcg gaacggacca gtcgagcagc
     5401 ggctcgtcca cgggggcggc aggccccgtc aggtcgcggg cgagccggtc gagccaggcg
     5461 ccgtaggacc tgcggtccag gagtccgcgc agcactgccc gccggggcca gcggtatttg
     5521 gcggcgtacc cccgtatcgc gtgcagcgcg acgaaggggt gggtgagcag cagcgtgtgc
     5581 agccgggcgg gcgtgccggc caggagttcg tcgccgccca ggccggtgag gtggaggccg
     5641 gatccgcggg ccgcggccat ttcgatgatg cccatgcggc ggttgcggtc gaccgtgatg
     5701 atgctgggcg tgtcgaggac gtccccgagg gtgtcgatgc cgtcgaacgt catggccacc
     5761 tcgtgggccg gtacgacgtg gtgttcgacg ccttcgagcg ccgatacggt gcgctcggcc
     5821 cagtacacgt cgtcgccggc ctcgtcgtgg acggcggcgg tgtaggcgac gaccttggcg
     5881 ccgccgcggg ccgcggtgca gcacaccgcc gtggagtcca ggccgcccag gtcggatgtc
     5941 accaggtcac gtccgcgggt gcgtacggcg accgcttcgg tgagggcttc cctcaggtgc
     6001 acggcgcccg cggcgagcgg gacgacgggg tcgggcggtg accaccaccg ggtggtgcgg
     6061 gcccggcctt cggtgtcgag cgtcaggcgg tggccgccgg gcaccgggtg cacgccccgc
     6121 cacagggggt cgccgtgcca cgggtacggg acgcccgaga cgagcatttc gagggcgatg
     6181 cgatgctcgt cgaggccggc acccaccagt tcggcgagca cgtcggcccg gtcggatgcc
     6241 acggtcaccg gccggccggg ggttgcggcc tcggcgtgga acacgcgccg cacggcgacg
     6301 gcaccggcct gcatccggac ccggccggcg cagctggcca tcaggtggaa gctgccggcc
     6361 cgccctgcga ggcggtccag cgcggacgcc gaagcggtgc cggtcgccga ggcggcggcc
     6421 cgttcggcgt cgagtgccgt gacggcgtgc tccccatcac cgcgacccgc gtgtcccggt
     6481 gcgtgcccgt cacgaccgtc cccggggtcc agcggcccac gatccagggc cgtccggagg
     6541 cgtggagcag gctgcgggag gcgaaggaga gcgccttggc ggcgggggcg gccgccgcgt
     6601 cgctgtcggg caggacgacg aaccaggaga gcacggactg gagcgagagg gactgatcca
     6661 tggggaacgg ccttccgtcg gcgacgtccg ggacggaaaa agcgggtggc ggctggaagc
     6721 cgccacccgc tctcaggtca gaggacctgc ctgccgccca ggacgtcctt cgggccgtgg
     6781 tccttgaggc ccgtcacctt gcggaagctg ccggccttgg cgagggtggg acgctcgtac
     6841 gtcatgaccg ttctccttct gtccgaggga cgatgatgtg actacgacag gcgcctgctg
     6901 cggagtccgt tgaaatccgt accggatatc cgagcggtgg atccgcggca ctcaccgcaa
     6961 gtacgacaag cacatcgtca caacgtccca ggcccggcta tggggttacg ccgggggcgc
     7021 gccgcgctgc cgcgccacgg gcacacaacc cctcacgcca cctgcgaaaa cggaaaaatc
     7081 cgtgagaaga atcgttaatc tttaggggga gatcaagcct ctcgcgccgg ttccaccccg
     7141 tgccacaccg tcgtcacgtt gcagaattcc ttgatgccgt gacccgagag ctcgcgtccg
     7201 tatcccgaac gcttgacccc gccgaacggc aatgcggggt gcgaggcggt catgccgttg
     7261 aagaagacgc ctccggcctc gatatcccgc gcgaaacgtt cctgttcctc cgcgtccgtg
     7321 gtccacacgt tcgaactcag cccgaagggc gtgtcattgg ccagctcgac ggcctcgtcg
     7381 acaccgtcca cgcggtagac ggtcgccacc gggccgaacg cctcctcgcg gtggatgcgc
     7441 atgtcggggg tgatgccggt gaggaccgtc ggctcgtaga accagccctt cggccgttcc
     7501 ttcggccgcc ggcccccgca cacggccgtc gcgccgcgcc gtacggcgtc gtccacgagt
     7561 tcctcgaggt cggcgcggcc ctgctcgctg gccagggggc cgacgtcggt ggcctcctcc
     7621 atggggtcgc cgacggtcag cgcggccatc cgggcggcga agcgttcgag gaacgcgtcg
     7681 tggacgtcgg cgtggacgat gaagcgcttg gcggcgatgc acgactggcc gttgttctgg
     7741 acgcgggccg tgaccgcggt ctgcacggcc cggtcgaggt cggccgacgg catcacgatg
     7801 tacgggtcgc tgccgcccag ttcgaggacg gtcttcttca cctcgtcacc cgcgatcgag
     7861 gcgaccgagc gcccggcggg ctcgctgccg gtgagcgtgg ccgccgcgac cctggggtcg
     7921 cgcaggatgc cttcgaccgc gcgcgagccc acgagcaggg tctggaaaca gccgtcggga
     7981 aagcccgccc ggcggaagag gtccccgagg tagagcgcgg tctgcggcac gttcgaggcg
     8041 tgtttgagca ggcccacgtt gccggccatg agggcggggg cggcgaagcg gatcacctgc
     8101 cacaggggga agttccacgg catgacggcg agcaccacgc ccagcgggcg gtagcggatg
     8161 cgggcgagcg cggccccgga gtcggcgacc tcgggctgcg gcgggtgctc gtccgccagc
     8221 agctccgccg cgttcgccgc gtaccagcgc atggccttca cacacttcgc cgcctccgcg
     8281 cgggcggcgg ccagcggctt gcccatctcg gtggtcatag tgcgcgcgat gtcgtcccgg
     8341 tcctcctcca ggaggtccgc ggcccggttc agcaggcggg cccgctcgcc cgggtccgag
     8401 gtgcggtagc ggcggaacgc cgccgcggcc gcggccaggc actgctcgat ctcctcggct
     8461 cccatctcct cgaacgtcct gagcgtctcg ccgttcgcgg ggttcaccgt tgcgatgggc
     8521 atggggggcc tcctcaaagc tcgatgcctc gaccagtgtg cgccggggcc cgctcagcgg
     8581 tacggggaca cgcggcgcgg accggcagat cccggcctcg cgcggaccgg cggatcccgg
     8641 ccttgcgcgg accggcggat cccggccttg cgcggacctc accccgtgcc actctccccc
     8701 gcgcggacct caccccgtcc tgcgctcccc cgcatcgtcc gtgacccgcg gctgaccggg
     8761 cagcagcccc atcgcctcct gcgtaccggc accccgcccg ctctccgccg gatacgggca
     8821 cggcagcgca cgggagcggt acagccaggc caccgcgcaa ccggaggcca gcccgaagaa
     8881 caggcacgac acccacggca gccaggcccg cccggtccgc tcgccgacgt ccatcaccca
     8941 gccgatcacg gcgttgccgc cggccgccgt cgccccggag agcacgtaga acaggccgaa
     9001 gtacgttccg gtcagccgct cgctgccgaa ccgcgggatc agccccagga ccggcggata
     9061 ggcgaccatc ccgccgaagt acagcagcag cgaggcggag accatgacca ggagccgcgg
     9121 caccacgtcg cccgcgccgt cgggcggcgc cgcgtcgcac accagcagcg gcgggacgaa
     9181 gccgagccct atgacggcga tcccggcccc cacccagcgc atcccgccgc cgcgctgctc
     9241 caggccccgg gtgatccgga actggaacag caggttggcg accgccccgg cgctcagcag
     9301 caacgcttct gccccgcccc agcccgaggc ctgctgcgcc ccgtccggca gcagcatgta
     9361 gagctggttc tccatgatga acatgccggt ggtggcgaag gcgaagcaga ggaaccggcg
     9421 gttgccgagc acctcgcgca gctcccgcag gatgggatcg tgctccgctg cgacctcgcg
     9481 cgccggcagc acgaacgcct gggcgaccgt cagcgccgcg aacagcccgg ccgccgtgag
     9541 ggcgcagatc cggaagtcgg tcaggtagag cacgctgccg gcgagcagcc ccacgagcga
     9601 gccgatggtc gcgaagacgt tgagcagggc gaacgcctcg gccttccgct cccccgactc
     9661 gtgcgagaca taggagcgca cggccgggta gaagagcgcg gcggcgaggc cgctgattcc
     9721 ggaggcggcg agcagcaggg gcagggagtt accgaaggaa aagagcgtga agcccacggt
     9781 ccgcagggcg cacccggcga tgatgaccac gcgcgggccg agccggtcgg ccgccgtgcc
     9841 gccgatgatg tacatgccct gctggctgag gttgcgcact ccgaggacca gtccgacgat
     9901 ggccgccgac attccgagat catgcccgat ataggtcgcc agaaagggaa tcagcagata
     9961 gaaaccggca tcgatgccgc actggttgat gaatagcaat cgcaatgcga gagggcactc
    10021 gcggactgcc cgaagagttc tcatgtgcgt ccctgcagtc agcccgagcg tggggacatc
    10081 atgggttccg gccccggtcg ggagccggta cgccccggcg aatggctcgt cagcccggat
    10141 tccacgcacg ggagcagacg cactcggcga cttggcggag cgaccgatca tatgcttggg
    10201 tcgacttgtg cccggcagct ccccggccgc gctcggaagg gtactcagcg gatacgttag
    10261 caaagccgac ctgttatcaa cagggtgaga ttccgggctg cggcagcgcc gggagcacac
    10321 ccggcacaag gaaattgcgc ccggctcttc cttcgagagc tggaactctc gtgccccgct
    10381 attgcgttca cgggaagcgt ttgcgggagg cgtttacggg aagcatgcgg cggcacaccg
    10441 cccgaacccc tgcccctccg gtcacacccg cccttgcgag ccgtccggcc cgggcccgcc
    10501 actcccccgg ggcaaccggc cttcccccgc acgggtgaaa accgcgactg aattcccgta
    10561 aacagaacac ggattctccg ccgaatccat tcttcagacc acagcccgcc cgaagatgcc
    10621 gaaagaccca cgtgcagcgg gcaacttcgc gacgatgtcc gtatgaacga ccggaacttc
    10681 ttggagcgca ccaagcagtt cgcgctctac ctggccgagc tccagacaag cctcgacccg
    10741 gagtcctacg cactgctgct gcggatcctg aagggcttca acgaggcgat cgtgcaccgg
    10801 gagcgcgggg tcgacattcc cttctccccg cgcgaaaaag agttgttcac acctgagttc
    10861 ggccgggtcc tcaccagact cgccggattc ctcggaccgc agggcatgca cgtcgtggtc
    10921 ggcacccacg gcgcccccgc ggacggcgga ccgccgggcg gcgcgtaccc cggcgaccac
    10981 ggcacggacc ccggcgacca cgaccggcgc gagatcgacg ccatcgcccg ggcgagtggc
    11041 atgcgcccgt aaccgcccgg cgcatcgtct acagtctcgt ccgccgtccg cagggccgcg
    11101 ggcggcgggg cacccgcccc gaccccgatc atgggaggcc acgccctcgt gcccgcttcg
    11161 cccgccgccg acctgaccac cactctggcg gtcaacctcc tcgacgcctc ctccctgctg
    11221 accgccttcg gcgccctcgg catcgccgtg gtgatgttcg ccgagaccgg cctcctgatc
    11281 ggcttcttcc tgcccgggga ctccctgctc ttcaccgccg gactgctcgc ccgcagcggc
    11341 gcccactccg gaccgcacct ggacctcgcg caggtcctgg ccgcctccgc ggccggcgcc
    11401 ctcaccggcg cccaggtcgg ctacgtcctc ggacggcgcg gcggccgggc gctcctcacc
    11461 cgctcccgca gccgcaaact ccacgagggc gccgagcgcg ccgaggagct cctcggccgc
    11521 tacgggcacg cccgcgccat cgtcctcgcc cgcttcgtcc ccgtcgtgcg cacggtcctc
    11581 aaccccctcg ccggggccct gggcgtcgca ccccgcacct tcaccctctg gcaggtgacc
    11641 ggcggcgtgg tgtggaccgt gggcctcgtc ctcgccggct acgggctcgg ctcggccgtg
    11701 cccgacgtcg accgctacct gctgccgatc gtcggcgccg tcgtcgtact gtcgctgctg
    11761 cccgtcgccc tcgaactgct gcgcagccgc gggcgccgca gggccgtcac gccggagacc
    11821 agccccgctc ccgcacccaa cgcccggcaa gagccgtgaa cgcccgcgcc gcggcactcc
    11881 ggtagcccgc ctcccggcac agcagcgtga cggtgcgcgc cggaagcggc ggcgcgaccg
    11941 gcacggggtg cagctgccac ggcccgccgg tgatggcctc gggcagcacg gtcgccagcg
    12001 cgctcctgcc gacgatctcg gtgagcgcgt tgatcgaatc ggcctcgacg gcgatgcgcg
    12061 gcgtgacgcc gtgctcggcg aggaaggcgt cgatgtgccc ccgcgtcgca aagtcgcggc
    12121 tgagcagggc cagcggatgg tcgccgagct cgcccaccgg cagcggctcg ctgcggcccg
    12181 cgcaggggtg atccggcccg acgacgaggc cgagcgtctc ggtgaacagg ccggtgccgg
    12241 cgacccccgg cagatgcgca ccgctgaacg cgatgcccaa gtccagctcg tcggccagga
    12301 gcgccgcttc gatgcggtcc tgcgccatct cacggaggtc cagggcgatc cccgcgtgcc
    12361 gggcgtggaa ctccgccacc agcgggccga tcagatacgc ggtgaacgtc ggcgtcacgg
    12421 ccagccgcag actgccccgc gagaggtcct gcacgtccag cacggcgcgc tcgcccgccg
    12481 cgagatcctg cagggcgtgg cgggcgtacc gggtgtacac ctcgcccgcg tcggtgagcc
    12541 gcacggtgcg cccggaccgg tcgagcagct ggacgcgcag ggaccgctcc agttgcttga
    12601 tctgctgcga cagcgtcggc tgcgagatgt gcagggcctc cgcggcgcgg gtgaagttcg
    12661 cgtgctcggc gacggccagc agataccgga ggtggcgcag ttccatgcgc gcaactatag
    12721 acagcgccga tggccgtacc gggtcacagc ggcaaccacc tatagggcct ggcggaagga
    12781 gctacggggt cctacgggct ctcagccctc cccgatcccc gcgaaccacg tggccccgtt
    12841 ctcggtcgcg tgcttgatgc gccgtaccgc gaactcctgc atcggcggca gggcgtccgg
    12901 ctcgaaccag cccacttcca aggactcgtc gtcattgacg cacgcctcgc cgtccaccgc
    12961 ccggcacagg aagcaggtgt ccatgaactg gcagacgtcg ccgttggggt aggtgaccgg
    13021 atcgagggcc tccaccacca ggacgcgctc cgccacgcaa cggacggccg tctcctcgta
    13081 cacctcgcgc acgatggccg ccgccggctg ctcgccggga tcgggaatgc cgccgatgat
    13141 cgcccactgg ccgttgtccg agcggcggcc gagcagcaca cggccctcgt cgtcgaggac
    13201 tacggcgctc acgccgggga tccacaggag ctgacggccc gcggtcgcgc ggaggtctcg
    13261 gatgaaatcg ggggtgggca tacgcaggag cctagtggcc cgcggaggcc cgccgttcac
    13321 ccaggtggcc gcatggacgg cggagaaacc ccagttcagg ccggggtcag gcgggagcct
    13381 tgcgccccgg ggcgagccgc agggcctgac ggatcacccg tacggcggat tctgcgtcgt
    13441 cgaccgtgac cgtgaacgtg cggccgtcgc ccaggcgtag gacgaatccc tcaccgcgcc
    13501 gcaccacgac ggccgtgccc ttctccgggc gccagcgata gccccagcca ccccactgcc
    13561 gcggcgtgac ccggggagcg aagtccgcat cgaccacatg ggcgagcagg atgcgggtgc
    13621 gcgggacgcc gatgtgcccg cagcgcacct ccagcgcttc cctgtcgacc cgtacggcca
    13681 catggacgaa cgccagcgtg ccgtagagca tcagcaggcc cgcggcgaca cagccgacga
    13741 ccgccatgac gagaggggcc agacctgagg tccatgcgct gtcgacggcc agctcgacgc
    13801 cgagcgccag gcaggcggcg ccggccgcgg caagcagcca ctggacgcgg ttcgtcgcgc
    13861 gacccgtcca gacggcgaga tcctcggcgc cgtcggccgg ggaaggggat ccggccgcgc
    13921 ctgtcttctc gtcgccctcg tggccctgcg ggcccgtgga atggtccctc atacgaggca
    13981 gcctactcac cttccgccac catggcaggg gtgatgcacg gaccgcaccg gatcgggtgg
    14041 tcagggacgg acggcggcgg cctcgacgag ggagcgtccc tcggggtagg tcagggaggc
    14101 ggcttccatg gcgccctcac ggccgccgag cagcactgtc aggtgccccg acggctcggc
    14161 gtcgggggcg ggagcggccc cgatccggcg cagcgcctgg gccgcgacgg cctccgcgga
    14221 gctgtacagg gtcaggtgcg gcacggccgg cgacagcagc ggctgtacgg cttcgcgaat
    14281 gcgctccgcg accagctcgt aatgggtgca gcccaggacg acgcccctta cattgcgcgg
    14341 cgtgagcgcg gccgccgcag cgatcgccgc gtccgcggcc gccatgtcgg cgtgctggac
    14401 ggcatccgcc agacccgggc agggcacctc cgtgacctcg acaccggccg cgaagtcacg
    14461 gatgagaccg cgctggtacg ggctgccggt ggtggccgga gtggcccaga tcgccacggg
    14521 gccgccggcc gccgcggcag gcttgatcgc aggcacggtg ccgatgaccg gcagctccgg
    14581 ctccagctcc gcccttatcg ccgccagcgc gtacaccgat gcggtgttgc aggcgatgat
    14641 cagcgcgtcg gggccctgct cggccgcggc ccgggcacag cccagggcgc gcgcgatgac
    14701 gtcctcgggc gtacgggggc cccagggcat gctggcgggg tcggaggaga ggaccagatc
    14761 ggcgtcgg
//
