LOCUS       NZ_BMUT01000024        17229 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces hiroshimensis strain JCM 4586 sequence24, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUT01000024
VERSION     NZ_BMUT01000024.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces hiroshimensis
  ORGANISM  Streptomyces hiroshimensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 46970)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 90X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650335.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:32:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,830
            CDSs (total)                      :: 6,745
            Genes (coding)                    :: 6,620
            CDSs (with protein)               :: 6,620
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 25 of 125
            Pseudo Genes (incomplete)         :: 107 of 125
            Pseudo Genes (internal stop)      :: 4 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:07:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 29741
            Orig. end                         :: 46970
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUT01000024.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..17229
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lanthipeptide-class-iii"
                     /region_number="1"
                     /rules="(micKC and not PKS_KS)"
                     /tool="antismash"
     cand_cluster    1..17229
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(micKC and not PKS_KS)"
                     /kind="single"
                     /product="lanthipeptide-class-iii"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..17229
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[10000:12577](-)"
                     /cutoff="20000"
                     /detection_rule="(micKC and not PKS_KS)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-iii"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(10001..12577)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(micKC and not PKS_KS)"
                     /neighbourhood="10000"
                     /product="lanthipeptide-class-iii"
                     /protocluster_number="1"
     misc_feature    complement(157..171)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 17.56"
                     /tool="antismash"
     misc_feature    complement(442..457)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 21.54"
                     /tool="antismash"
     gene            complement(533..2170)
                     /locus_tag="IE241_RS33100"
                     /old_locus_tag="GCM10010324_67430"
     CDS             complement(533..2170)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019544803.1"
                     /locus_tag="IE241_RS33100"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_67430"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_229900194.1"
                     /transl_table=11
                     /translation="MRATWRGLPTAQHEVGWEPGLVVPAADGSPLRTDHYFPRTQGDFP
                     TLLVRSPYGRGLPWSPMYGVLFAEQGFHVVIQSCRGTGGSGGRFDLWRNEAADGLATVS
                     WLREQPWFTGTLGTVGPSYLGYVQWALALDPPPELKAMVVQVGLHDPYALFHAGGALHL
                     ENALVVGAGMTYQHQGVLPFTRAVLRLQRRLREITTARPLRGAHVSALGEVPWLDDVMT
                     HPDAGDPYWHGASLAGAAERLDIPTALITGWHDALADQTFEQYGRLRRAGCETSLLVGP
                     WTHTSALQQGWPEVFSESLAWLRAHLCDDPAGLRPTPVRVHVGGEGTWRDLDDWPPASG
                     MTAWFPAANGALTRQTPGEPTPLTTFRYDPADPTPSLGGPVLSRGAGPRDNKALEARDD
                     VLTFTSAPLAGPVDVLGPVSARLRLSTDTGCADVFARLCDVDAQGRSFNVCDGLTALRT
                     TGQSLSEVTVPMSSTAHRFAAGHRIRLQISGGAHPRFARNPGTGEPAADATGLTPVHIT
                     LHTGSALTLPGAAEAGGTSGGSSGGAER"
     gene            2419..3147
                     /locus_tag="IE241_RS33105"
                     /old_locus_tag="GCM10010324_67450"
     CDS             2419..3147
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1239: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003990943.1"
                     /locus_tag="IE241_RS33105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_67450"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_229900186.1"
                     /transl_table=11
                     /translation="MTRGDGAGAAGVDPQQLWLTPARSGRGRKPAFSRRAITAAAVDLA
                     DAEGLEAVTMRRVAARVGAGVMSLYNYAPDKETLLELMVDHVSGELPTTEPLTGDWRTD
                     LKAIGHLQRALMLRHPWLPAALSARRTLGPHTLAFLERALAALRPTRLDGAAKVEVFAQ
                     LTGFVAAHVAHEIAQAAAAQSPERAAAEARYLAAVAADGHHPELAEALAAPGRPLTPEA
                     TFARFLGRLIDGLDTASDAD"
     gene            complement(3178..3969)
                     /locus_tag="IE241_RS33110"
                     /old_locus_tag="GCM10010324_67460"
     CDS             complement(3178..3969)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010351867.1"
                     /locus_tag="IE241_RS33110"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_67460"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_190025576.1"
                     /transl_table=11
                     /translation="MTWAHLRHGAAHQPSAQHPPAAPPAAAAMDLSKVYGSGARRVAAL
                     DRVSVSFQQGHFTAIMGPSGSGKSTLMQCLAGLDTPTSGTALIGDTRLSSLSERQLTRL
                     RRERIGFIFQAFHLLPALTVAENITLPLAITGRRPDGQWLDQVVTTLGLTDRLGHRPGE
                     LSGGQQQRVACARALAGRPDLVFADEPTGNLDTPTSAEVLSLLAHLTHRMGQTIIMVTH
                     DPAAAAYTDRVVFLADGRITDELHRPDADQILARMKRFNTP"
     gene            complement(3966..6164)
                     /locus_tag="IE241_RS34735"
                     /old_locus_tag="GCM10010324_67470"
     CDS             complement(3966..6164)
                     /GO_function="GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_S9"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012546.5"
                     /locus_tag="IE241_RS34735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_67470"
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="WP_190025577.1"
                     /sec_met_domain="Peptidase_S9 (E-value: 6.1e-43, bitscore:
                     138.3, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADHVPAAGDGVRPDRETGEPADPYRWLEEDSPRTRAWLAAQQRL
                     LAAHRERTDDRTWQSLLAGIETAGAGRLLSPPVEAGGMLFRHVLGASGSELLTATDPHG
                     RSRTLLDTGDRPSPTRTAGWHADPHGRALLVQLHHDGHENGGLHLVPADGTTPAVYLSD
                     ASPHPAVACAGDLLLYSAGTRTEHSLHVRSLRNDRAPSPAPGPDRTVELPVPGPVRITL
                     HSGPGGHLLLRTRTAKAPSAQWWCTRWDGHGTPDWQPLPLHGLRITAFALGTDHLYLAT
                     GQDISALALPDATRGHITPPAPLPTGHLPGEIKALRVLGPGPAPCLAVLRQHGTARRLD
                     FQPVGTRTESLAAAGPDPAPGAGTGADTSAGSESPGSTVLTWHARLRIGPLSYGDDGRP
                     GDALWILADDPRDGCWSHRLTPGAPPLTPPAGHSPLRTTTAVSSDGTALPVTLCDPPPG
                     GKGPAPVLVTVYGGFGIPLEPSWDPILAAWLTAGGRVAWVHARGGGEFGADWAAAGRGA
                     GKSHTVDDLCAAARTLVAQGEAEPGQLAGLAASNGGLVLAAAVLRAPRLFSAVACAAPL
                     TDMARYTSGGGLGRLWHDEYGDPADPAALAALLAYSPYHRVRQGESYPATLLITGGNDA
                     RVPPWHAWKLCAALQEATPGPQPVLLDHQCDTGHHGRTGTAATALGARVLALLATRTGL
                     TGPTRPSPPPPATDVNAPSPLRAHRQPTALAPSSRSTA"
     misc_feature    complement(5217..5219)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(5583..5585)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6157..7503)
                     /locus_tag="IE241_RS33120"
                     /old_locus_tag="GCM10010324_67480"
     CDS             complement(6157..7503)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1020: major
                     facilitator transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF017760.5"
                     /locus_tag="IE241_RS33120"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_67480"
                     /product="MFS transporter"
                     /protein_id="WP_190025578.1"
                     /transl_table=11
                     /translation="MKQPKQKLGRRFWLLFSGEAASAVGTAASVIALPMVAYQVSGDVR
                     HAGFTSTALSLGIVAARLPAGVLADRYPRRTLLLLGNAAGALVLGTLAVLRATGAVHPG
                     ALLVAAFLLGAVGSTLAPVENVAVRTFVQANLLPRALALVQSRAALALIIGPLVAGVLL
                     KANAAWVFAADALTYLTAACCAFLLPAKAGPTGTAQPPLKAVTEGMRFLWRSPFLRYGA
                     LNATVLNLVFNGLLIVVIATCDRTGAGTVAVGVQTACFGAGAVAGSLVAAPVARRLPAG
                     RAIALATAVIALALLGFATVRSTWGAALMLAIATSGGPVITVVLSSMQMRITPPRLQGR
                     VHSASGFLSQAIAPLGPTLAGAGAHAFGLGTTVTAAAALVVLLAVTGWFTTARHAVAAD
                     AQQALELQEIMDAHQPVDAAAEKHSDTQKSLDAQEARAGNSAAVEANRG"
     gene            complement(7500..9422)
                     /locus_tag="IE241_RS33125"
                     /old_locus_tag="GCM10010324_67490"
     CDS             complement(7500..9422)
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF014717.5"
                     /locus_tag="IE241_RS33125"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_67490"
                     /product="FtsX-like permease family protein"
                     /protein_id="WP_190025579.1"
                     /transl_table=11
                     /translation="MRRLFLAEARVHPGRLAGAAVACAVTAAGVGACTLLLIAAGRADF
                     PARSVAAAQAEDARSLLSLLLSMLLMSAVVVIGSTVSLWTGQRLGQFAVLRALGVTAGR
                     LRRLVAGDVARLAVAAAAVGALGMLPLAHAGRRLLVDRELFPEAASLPSSGQVWGTAVG
                     VCIATGAVAVLSAMASVLAAGRVSPGALMKDTEGSLASRRSRARLLTGLAMALMLCVPL
                     LCVMAFTQLPATVRAAVAPGLALMLIPTLAVLAPWIVPPLSRPVCALLRIADRRVGRIA
                     AAGLRAAPARTTAMAVPVLLAVGIAVCLLGTGATMGQAVARQTADGLRADGVVTARPGH
                     RLPAQPAAASGGPGALSGVTATVLVSTQITAPPTSLDDQPSPVEAWGADGSSLVKSLDL
                     KEREGRLSDLREGTFASGADQAEAHHWRLGQKVRLALADGSRPELTLAAVYERDLAFPA
                     FVIARTTALEHTREPYADRILLSGAYRSWPKEPGQTVASRAQYLDRLQPRSPADDLASR
                     LIVSVVSGYALLAAANTASLAQRDRRAQRAHLRALGLSRGQLLRCVLYEALGAAAVGAA
                     LAAATAVACLVPFAAALGTGALPALDVPWTVAVLAAAVLAVALPSVMTAHPMSTVQRQF
                     ARRAV"
     misc_feature    complement(8196..8198)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(9580..9592)
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.62"
                     /tool="antismash"
     gene            complement(9583..9672)
                     /locus_tag="IE241_RS33130"
     CDS             complement(9583..9672)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF038154.3"
                     /locus_tag="IE241_RS33130"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class III lanthipeptide"
                     /protein_id="WP_190025580.1"
                     /transl_table=11
                     /translation="MQNILALQELDEETVLDAPWSSVSIAGCH"
     misc_feature    complement(9658..9673)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 19.5"
                     /tool="antismash"
     gene            complement(9739..9828)
                     /locus_tag="IE241_RS33135"
     CDS             complement(9739..9828)
                     /codon_start=1
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE241_RS33135"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /product="class III lanthipeptide"
                     /protein_id="WP_190025581.1"
                     /transl_table=11
                     /translation="MRNILALQELDEETIVDAPISTISLGGCH"
     CDS_motif       complement(9772..9828)
                     /prepeptide="leader"
                     /note="peptide class: lanthipeptide"
                     /note="predicted sequence: MRNILALQELDEETIVDAP"
                     /locus_tag="IE241_RS33135_lanthipeptide"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
     CDS_motif       complement(9739..9771)
                     /prepeptide="core"
                     /locus_tag="IE241_RS33135_lanthipeptide"
                     /peptide="lanthipeptide"
                     /predicted_class="Class III"
                     /score="-2.10"
                     /molecular_weight="933.1"
                     /monoisotopic_mass="932.5"
                     /core_sequence="ISTISLGGCH"
                     /aSTool="lanthipeptides"
                     /tool="antismash"
                     /leader_sequence="MRNILALQELDEETIVDAP"
                     /leader_location="[9771:9828](-)"
                     /alternative_weights="951.1"
                     /alternative_weights="969.1"
     misc_feature    complement(9814..9829)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 17.07"
                     /tool="antismash"
     gene            complement(9905..9994)
                     /locus_tag="IE241_RS33140"
     CDS             complement(9905..9994)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF038154.3"
                     /locus_tag="IE241_RS33140"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="class III lanthipeptide"
                     /protein_id="WP_190025582.1"
                     /transl_table=11
                     /translation="MQNILALQELDEETVLDAPWSSVSVAACH"
     gene            complement(10001..12577)
                     /gene="lanKC"
                     /locus_tag="IE241_RS33145"
                     /old_locus_tag="GCM10010324_67500"
     CDS             complement(10001..12577)
                     /GO_function="GO:0004672 - protein kinase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /GO_process="GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /gene="lanKC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lanthipeptide-class-iii: micKC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pkinase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF038151.1"
                     /locus_tag="IE241_RS33145"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_67500"
                     /product="class III lanthionine synthetase LanKC"
                     /protein_id="WP_190025583.1"
                     /sec_met_domain="micKC (E-value: 1.1e-201, bitscore: 663.5,
                     seeds: 18, tool: rule-based-clusters)"
                     /sec_met_domain="Pkinase (E-value: 6.5e-16, bitscore: 50.0,
                     seeds: 54, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDPSYEAYTIADSTWYEPLERCQDSDSLFSLSVRELPQGWRREHQ
                     GVWEFLMPGDTGKLPEQGWKIHVSATPGAAEETIEAAWTVCRQLELPWKFLRSRRIVAA
                     MNTKYAHRAASGKAVTVYPRTTDELRRALDELETVMRGRPGPYVLSDRRWNDGPVSVRY
                     GAFILMWCERADGTRVPALRTPQGQLVPDHRRPVFTLPPWADVPDFLAERPAADGESAS
                     LGGYEVRKALHFSNGGGVYLAHDGEGRQVVLKEARPHAGLDANGMDAVDRLRTEDAVLD
                     GLGHLPFVPRRYGAFTAWEHHYLAMEYVEGETVSAWLGREHPLTRHRPTEAARTRFAAS
                     VVERMEQVAECVRALHEAGVAFGDLHHRNILIRPDGTVALVDFELAAPVGSVRSAALGA
                     PGFADPALTDPREADLFALGCCQLAALVPLTVLTLRNPRSVASLIELARASFPSLPAEF
                     IRQMTERLALSPALRPHLPPPGTLAGPPPPLTPPSTDTLLRGIARSADTGRKDRRYPCD
                     VAGHRPGAALGLAYGTPGVLWAQHAAGTAVSDDDVEWLAGAVRRAPLDTPAGLYDGMAG
                     AAWLLHRLGHPQGANLVDRLLAGPLPASPGLFSGLSGIAHLFLDVGLKDEALALALRVR
                     ERVGTKGLLDRPGLMHGWSGPAVLFARCAEVTGDLQWAAAAERAVRCDLAHARDVDGML
                     QMHSSSRLLPYLAEGSAGVAMAALALPPPQAAALGADGIVAGAARAGAVEVVLQGGLFS
                     GRSGLAYLLSHALSRTGRWQAELDSHLRALTLHLAPYEDAHVLHGDQLVRLSADLATGS
                     AGALIALAAAKNPGSRLLPGAHPTCGWPPAGSDAPDHPALRAREGR"
     misc_feature    complement(11162..11164)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(12749..13498)
                     /locus_tag="IE241_RS33150"
                     /old_locus_tag="GCM10010324_67510"
     CDS             complement(12749..13498)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024248.5"
                     /locus_tag="IE241_RS33150"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_67510"
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_190025584.1"
                     /transl_table=11
                     /translation="MSLRSAVQDFQQMAVAPHWGRIRAYLGEVRDTLRDIMCRDGVEVL
                     LDSLGPGIRWNPPALEIAGVRSGEMELDGTGLVLAPSLFLQRAGHVLPPARQGVHRAPV
                     LVFPDRPGPEELTQLLGGGRAGGPHCGPDSLAALVGRTRAAALRALREGCTNAELAQRL
                     GVSAAAVSQHMAVLRAAGLVSTRRSRSHAIHTVTHLGRLLLRGEAAERRPAEPTLRQEP
                     TLRHTAFQQGRERREPAAAAAGRQISA"
     gene            14016..15065
                     /locus_tag="IE241_RS33155"
                     /old_locus_tag="GCM10010324_67520"
     CDS             14016..15065
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313575.1"
                     /locus_tag="IE241_RS33155"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_67520"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_190025585.1"
                     /transl_table=11
                     /translation="MEALVVRLSQLDAEAEGAIRVVMFYDTLMRRRVDLPALARASASL
                     AECVAGIRLFGTGRLIRIGPDGREAPAAEALAPEALVSTTVPITLDDEAIGAVWLERSG
                     PPGPFDDLLLDRLALAVSAAVERHGPAFTTMADPALVELVISPGSDEAARARALRLMGF
                     SAGLPVRVVAVRSEIPLDQVGGSICAARPVKAAQVADVGVILAATVAADRLPAGVRAGI
                     GIAGNPVLSWQQARTALRFTSPREPVVHYAGLGALALLAEIPQDVSRDNADVAAIARMA
                     GSPDDLETLDAYCTTGSLRRAADLLHLHHSSVARRLEHIGTTLGIGLTEPAGLVRARLA
                     LTAWRLLDD"
     gene            15355..16431
                     /locus_tag="IE241_RS33160"
                     /old_locus_tag="GCM10010324_67530"
     CDS             15355..16431
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004943491.1"
                     /locus_tag="IE241_RS33160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_67530"
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="WP_190025586.1"
                     /transl_table=11
                     /translation="MNRDLTLHDIIVAGIAVAAGLALALVLRVILRWLGVRALRTRWQG
                     DDIIVDVLRTLAPGCAVAAGVAAAAAALPLTSQVGHIVDEVVMACIVLIATFTAARVIS
                     GLVRSMAQSRSGVAGSATIFVNITRIAVLAMGFLVMLETLGISIAPLLTALGVGGLAIA
                     LALKDTLANLFAGVHILVSKTVQPGDYIRLSSGEEGYVRDINWRITVVEQLNNNLVIIP
                     NAKLAGTNMTNFSRPEQKLSVTVQVGVAYDSDLERVEQITVDVAESVMKDVAGAVPDHE
                     SAVRFHTFGDSRIGFTVILGVGEFSDKYRIKHEFIKRLHQRYRAEGIRIPAPARTVSLQ
                     QTGEPAIPHPRASLDQAS"
     gene            16764..>17229
                     /locus_tag="IE241_RS33165"
                     /pseudo=""
     CDS             16764..>17229
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004944770.1"
                     /locus_tag="IE241_RS33165"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="lytic transglycosylase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MRSTVSAYVRLPKIHKFSVAGIAATGAAVVAVAVAPHAFAADAAP
                     AAAVKPVAWSAEAFGETQQADLEKHADAAAKAGADAAAEAKAGKERAEKQAANRSTERK
                     AIAAKPAAAAKPAAKPAAPAKPAAKPAAPVKKAYADDLEGWIGQSLDILRA"
ORIGIN
        1 ccccggctgc agccgcgacg ccctccgcgg acgccaacgg caaggccatg acctggaccc
       61 tgctcgcgga cgggcccagc gggacggtgc aggtgggcgg cggcgccctg agcaacccct
      121 atcagggtga cacggccacg accacggcac tgcccgttct ctgcctgaga atcgacggct
      181 cgccggcccc cgccggcatc acgcccggct tctacgcggg ctgggcgcgc ggcgcggtcg
      241 cggccaccaa tccgatccgc ggcacccgcc tgacgagccg cgcggtcgcc gacggagtct
      301 gcgccaacaa cttcggagcg ggctggcgca tggccgagtt ccacgacggc agatacggac
      361 ccggcctgga gagctcgggt ggctggagct actgggccta cggggacgtc ccggccggca
      421 cccgtttctg gacggccatc aacgaccagc cggccaaccc ctggaactga cgcacctcct
      481 ccgcccgcgc cgggcgagac cgtgacgacc gctcaccgcc cggcgcggac ggctaccgtt
      541 cggcgccgcc cgacgagccg cccgacgtgc cgcctgcctc ggccgccccg gggagcgtca
      601 gcgccgagcc cgtgtgcagc gtgatgtgca ccggcgtgag gcctgtggca tcggccgccg
      661 gctccccggt gccggggtta cgggcgaagc gcggatgtgc gcctccgctg atctggaggc
      721 gaatgcggtg accggcagcg aagcggtggg cggtcgagct catcggcacg gtgacctccg
      781 agaggctctg tccggtggtc cgcagcgcgg tcagcccgtc gcagacgttg aaggaacggc
      841 cttgtgcgtc cacgtcgcac aggcgggcga agacgtcggc gcagccggtg tccgtggaga
      901 ggcgcagccg tgcggagacc gggccgagga cgtcgacggg cccggccagg ggtgcgctgg
      961 tgaacgtcag tacgtcgtcc cgcgcttcga gcgccttgtt gtcgcgggga ccggcgccgc
     1021 gggagagcac gggcccgccc agggagggag tggggtcggc agggtcgtag cggaaggtgg
     1081 tcaaaggcgt gggctcgccg ggggtctgcc gggtgagcgc tccgttcgcg gcagggaacc
     1141 aggccgtcat gcctgacgcg ggcggccagt cgtcgaggtc ccgccacgtg ccttcgccgc
     1201 cgacgtgcac gcgcacgggg gtgggacgca gcccggcggg gtcgtcgcac aggtgcgcgc
     1261 gcagccaggc gaggctctcg gagaagactt cgggccagcc ctgctgcagt gcggaggtgt
     1321 gggtccaggg gccgaccagc agggaggtct cacatccggc ccggcgcagc cgcccgtact
     1381 gctcgaaggt ctggtcggcc agtgcgtcgt gccatccggt gatcagcgcg gtgggtatgt
     1441 cgagccgctc ggccgcccct gcgagggatg cgccgtgcca gtacgggtcg ccggcgtccg
     1501 ggtgcgtcat cacgtcgtcc agccagggca cctccccgag agcggacaca tgcgctccgc
     1561 gcagcggccg cgccgtggtg atctcgcgca gacggcgctg caggcgcagc accgccctcg
     1621 tgaaggggag caccccctgg tgctggtacg tcatgccggc gccgacgacg agggcgttct
     1681 ccaggtggag tgcgccgccc gcgtggaaca aggcgtacgg gtcgtgcagg ccgacctgga
     1741 ccaccatcgc cttgagctcc ggcggcgggt ccagggcgag agcccactgc acataaccca
     1801 gatagctggg gccgacggtg cccagtgtcc cggtgaacca gggctgctcc cgcagccacg
     1861 acaccgtggc caggccgtcg gcggcctcgt tgcgccacag gtcgaagcgg ccgcccgagc
     1921 cgccggtgcc gcggcagctc tggatgacga cgtggaagcc ctgttcggcg aagaggacgc
     1981 cgtacatcgg tgaccagggc aggcccctgc cgtagggcga gcgcacgagc agggtgggga
     2041 agtcgccctg cgtgcggggg aagtagtggt ccgtgcgcag agggctgccg tccgcggccg
     2101 gcaccacgag tccgggctcc cagccgacct cgtgctgtgc ggtcggcagg ccgcgccatg
     2161 tcgccctcat cagccgtgag gagagcggcg gcttcccgga cggcgcgctc caggcggcct
     2221 tcgtggtcct cgtggccctc gtggtcgtcg tgtccctcgg cgcggcatcg tgcgcatgcg
     2281 gtgtcatcgc gtccccctcc atcgtttacg tacgatgtac gagaatagag gatgcttgga
     2341 tggtttccgc aaatcgcagc ggtgttgaca aggtgtgcag gcagcgcgcg ccgaggtgag
     2401 aagaggagcg aggagaacgt gacccgtgga gacggcgccg gtgccgccgg agtcgacccg
     2461 cagcagctct ggctgacccc ggcccggtcc ggcagagggc gtaaaccggc cttcagccgc
     2521 cgggcgatca cggccgcggc cgtcgacctg gcggacgccg aaggactgga ggcggtcacc
     2581 atgcggcgcg tcgccgcccg ggtcggggcc ggagtgatgt cgctctacaa ctacgcgccc
     2641 gacaaggaga ccctgctgga actgatggtc gaccacgtca gcggcgagct gccgaccacg
     2701 gagcccctca ccggggactg gcgcaccgac ctgaaggcca tcggtcacct gcagcgcgcc
     2761 ctcatgctcc ggcacccctg gctccctgcc gcgctgtccg cccgccggac cctcggcccc
     2821 cacacgctgg ccttcctgga gcgtgccctg gccgccctgc ggccgacccg gctggacggt
     2881 gccgccaagg tggaggtctt cgcccagctc accggattcg tcgccgcgca cgtcgcccac
     2941 gagatcgcgc aggccgcagc cgcacagtca ccggagcggg ccgcggccga ggcccgctac
     3001 ctcgcagccg tcgccgcgga cggccaccac cccgagctcg cagaggccct cgccgcgccc
     3061 ggacgacccc tcacccccga ggccacgttc gcccgtttcc tcggccgcct gatcgacggc
     3121 ctcgacacgg cctcggacgc agactgaagc acccggccgc acctccccgc ggcccggcta
     3181 cggggtgttg aagcgtttca tacgggccag gatctggtcg gcgtccgggc ggtggagttc
     3241 gtcggtgatg cggccgtctg ccaggaagac gacgcggtcg gtgtaggcgg cggcagcggg
     3301 gtcgtgggtg accatgatga tcgtctgccc catgcggtgg gtcagatggg cgaggaggga
     3361 gaggacttcg gcactggtgg gggtgtcgag gttgccggtg ggctcgtcgg cgaagacgag
     3421 gtcgggccgg ccggcgaggg cccgggcgca ggcgacgcgt tgctgctgcc cgccggacag
     3481 ttcgccgggg cggtggccga gccggtccgt gaggccgagc gtggtgacga cctggtccag
     3541 ccactgcccg tccgggcgcc gccccgtgat ggccaggggc agggtgatgt tctccgccac
     3601 ggtcagggcg ggcaggaggt ggaacgcctg gaagatgaat ccgatccgtt ccctgcgcag
     3661 gcgggtcagc tgccgctcgg agaggctgga cagccgggtg tcaccgatga gggccgttcc
     3721 ggacgtaggc gtgtccagcc ctgccaggca ctgcatgagc gtggacttgc ccgaccccga
     3781 gggccccatg atggcggtga agtgcccctg ctggaacgac acgctgacac ggtcgagggc
     3841 tgcgacgcga cgtgccccgc tgccgtacac cttggagagg tccatcgcgg ccgcggccgg
     3901 cggggccgcg ggcgggtgct gtgcggaggg ctggtgtgcc gcgccgtggc gcaggtgtgc
     3961 ccaggtcatg cggtgctcct cgaggacggt gcgagtgcgg tcggctgccg gtgcgcccgg
     4021 agcggcgagg gcgcgttcac gtcggtggct ggcgggggcg ggctcggtct ggtgggcccg
     4081 gtgaggccgg tgcgcgtggc gaggagggcc aggacgcgcg cgccgagtgc cgtggcggcg
     4141 gtgccggtgc ggccgtggtg gccggtgtcg cactggtggt cgaggaggac gggctgcggg
     4201 cccggcgtcg cctcctggag tgcggcgcag agcttccagg cgtgccaggg cgggactcgt
     4261 gcgtcgttgc ctccggtgat caggagcgtg gccgggtagg actcgccctg ccgtacgcgg
     4321 tggtagggcg agtacgccag gagcgctgcg agcgcggccg ggtcggcggg atccccgtac
     4381 tcgtcgtgcc acaggcggcc cagaccaccg cctgaggtgt agcgggccat gtcggtgagg
     4441 ggcgcggcgc aggcgaccgc ggagaagagg cgaggggcac ggagcacggc tgcggccagc
     4501 accaggccgc cgttgctggc ggccagcccg gcgagctgcc cgggctccgc ttcgccctgg
     4561 gccacgaggg tgcgtgctgc cgcgcacaag tcgtcgacgg tgtgcgactt gcccgcgccg
     4621 cgccctgctg ccgcccagtc cgcgccgaac tcgccgccgc cccgggcgtg gacccaggcc
     4681 acgcgcccgc cggcggtgag ccaggcggcg aggatggggt cccaggaggg ttcgaggggg
     4741 atcccgaagc cgccgtagac ggtgaccagg accggggccg ggcccttccc gccggggggc
     4801 gggtcgcaga gggtgacggg gagggcggtg ccgtcgctgc tgacggcggt cgtggtacgg
     4861 aggggggagt ggcctgccgg tggggtgagg ggtggtgcgc cgggggtgag gcgatggctc
     4921 cagcagccgt cccgtgggtc gtcggcgagg atccacagcg cgtcgccggg acggccgtcg
     4981 tcaccgtacg agagaggccc gatgcgcagc cgggcgtgcc aggtcagcac tgtgctgccc
     5041 gggctctctg agcctgcgct tgtgtctgcg cctgtacctg cgcccggggc cgggtccggg
     5101 cccgcggcag ccagggactc ggtccgcgtg ccgacgggct ggaagtcgag gcgccgggcg
     5161 gtgccgtgct gccgcaggac ggccaggcag ggggccgggc ccggccccag gacgcgtaag
     5221 gccttgatct cccctggaag gtgcccggtg gggagcgggg cgggaggtgt gatgtgcccg
     5281 cgggtcgcgt ccggcagagc cagtgccgag atgtcctggc ctgtggcgag gtacagatgg
     5341 tccgtgccga gtgcgaacgc ggtgatgcgg aggccgtgga ggggaagggg ctgccagtcg
     5401 ggtgtgccgt ggccgtccca gcgcgtgcac caccactgtg ccgagggcgc cttcgccgtc
     5461 cgggtgcgca gcagcaggtg gcctcccgga ccgctgtgca gcgtgatcct caccggtccg
     5521 gggacgggca gttccacggt ccggtccggg cccggggcgg ggctcggggc ccggtcgttc
     5581 cgtaaggagc ggacgtgcag actgtgttcg gtgcgggtgc cggcgctgta gagcaggagg
     5641 tcgccggcgc aggccacggc cgggtgaggc gaggcgtcgg agaggtacac cgcgggtgtg
     5701 gtcccgtcgg cggggaccag gtgcaggccg ccgttctcgt gcccgtcgtg atggagctgg
     5761 acgagcaggg cgcggccgtg ggggtcggcg tgccagcctg ctgtccgggt gggggagggg
     5821 cggtcgccgg tgtccaggag ggtacggctg cgtccgtgcg ggtcggtcgc ggtgaggagc
     5881 tcgctgcccg aggctccgag cacatgccgg aacagcatcc ccccggcttc caccggtggg
     5941 ctcaggagac ggccggcgcc ggcggtctcg atcccggcca gcagcgactg ccaggttcgg
     6001 tcgtcggtgc gctcccggtg ggctgccagc aggcgttgct gtgcggccag ccacgcgcgc
     6061 gtgcggggag agtcctcctc cagccaccgg tagggatccg ccggttcacc ggtttcccga
     6121 tccggccgga caccgtcacc ggccgccggt acgtggtcag ccacggttcg cctccaccgc
     6181 cgcggagttc ccggcgcggg cctcctgcgc gtccaaggac ttctgcgtgt ccgagtgctt
     6241 ctctgcggct gcgtccacgg gctggtgcgc gtccatgatc tcctgcagct ccagggcctg
     6301 ttgcgcatcc gcggccaccg catggcgggc cgtggtgaac cagccggtca cggccaggag
     6361 gacgaccagg gccgccgctg ccgtgaccgt ggttcccagg ccgaaggcgt gtgctccggc
     6421 tcccgcgagc gtggggccga ggggggcgat ggcctgggag aggaagccgc tggcgctgtg
     6481 gacgcgcccc tgcagccgcg gcggggtgat gcgcatctgc atcgaggaca gcaccacggt
     6541 gatcacaggg ccgccggagg tggcgatcgc cagcatgagg gccgcgcccc aggtgctgcg
     6601 gaccgtggcg aagccgagca gcgcgagcgc gatgacggcg gtggccagcg cgatggcgcg
     6661 gcctgcgggg agtctgcgtg ccaccggggc ggcgaccagg gatccggcga cggcgcctgc
     6721 accgaagcag gcggtctgca cccccacggc caccgtgccc gcgccggtcc tgtcgcaggt
     6781 ggcgatgacc acgatcagca ggccgttgaa gacgaggttc aggacggtgg cgttgagcgc
     6841 gccgtagcgc aggaagggcg agcgccacag gaagcgcatg ccttcggtga cggccttgag
     6901 cggcggctgc gccgtgccgg tggggcccgc cttggccggc aggaggaagg cgcagcaggc
     6961 cgcggtcaga taggtcaggg cgtcggcggc gaatacccag gcggcattgg ctttgaggag
     7021 cacgcccgcc acgagggggc cgatgatcag tgcgagcgcg gcgcggctct ggaccagggc
     7081 cagcgcccgg ggcaggaggt tggcctggac gaaggtgcgt acggccacgt tctcgacggg
     7141 ggccagggtg ctgcccacgg cgccgaggag gaaggccgcg accaggagcg cgcccgggtg
     7201 gacggcgcct gtcgcgcgga gcacggccag cgtgcccagc accagggcgc cggcggcgtt
     7261 gcccaggagg aggagcgtgc ggcgtgggta gcggtccgcg agcaccccgg cgggcagccg
     7321 ggccgcgacg atccccaggg acagcgcggt gctggtgaat ccggcgtgcc ggacgtctcc
     7381 gctcacctgg taggcgacca tgggcagggc gatcaccgag gccgccgtgc ccacggcgga
     7441 ggcggcctcc ccgctgaaca gcagccagaa ccgcctgccc agcttctgct tcggctgctt
     7501 cacaccgctc tccttgcgaa ttgtcgttgg acggtggaca tcggatgggc ggtcatcacc
     7561 gagggcaggg cgaccgcgag gacggcggct gccaggacgg cgacggtcca cggcacgtcc
     7621 agggcgggca gcgccccggt gcccagggcg gcggcgaagg ggaccaggca ggcgactgcc
     7681 gtggcggccg ccagcgccgc cccgacggcg gccgcgccga gcgcttcgta gagcacgcag
     7741 cgcagcagct ggcctctgct cagcccgagg gcgcgcaggt gggcgcgctg ggcgcggcgg
     7801 tcgcgctggg ccagggacgc ggtattggcc gcggccagca gcgcgtagcc gctgacgacg
     7861 gagacgatca gacgggaggc gagatcgtcg gcgggactgc ggggctggag gcgatcgagg
     7921 tactgcgcgc gggaggcgac ggtctggccg ggttccttcg gccaggagcg gtacgcaccg
     7981 ctgagcagga tccggtcggc gtacggttcc cgggtgtgtt ccagggcggt ggtgcgcgcg
     8041 atgacgaacg ccgggaaggc gaggtcgcgt tcgtagacgg cggcgagggt cagctcgggg
     8101 cgggatccgt cggccagggc cagccggacc ttctggccga ggcgccagtg atgggcctcg
     8161 gcctggtcgg cgccggaggc gaaggtcccc tcccgtaagt ccgacagccg cccttcgcgt
     8221 tccttgaggt ccagggactt cacgagcgag gagccgtcgg ctccccaggc ctccacgggg
     8281 gagggctggt cgtcgaggga cgtgggcgga gcggtgatct gcgtggacac cagcaccgtg
     8341 gcggtgacgc cggagagcgc tccgggcccg ccggaggccg cggccggctg cgcaggaagc
     8401 cggtgcccgg ggcgggcggt gaccacgccg tcggcgcgca gcccgtcggc ggtctggcgc
     8461 gcgacggcct gtcccatggt ggcgccggtg cccagcaggc agacggcgat gcccacggcg
     8521 agcaggacgg gcaccgccat ggccgtcgtc cgcgccggag ccgcccgcag gcccgcggcg
     8581 gcgatacgtc ccacccgccg gtccgcgatc cgcaggaggg cgcacaccgg ccggctgagc
     8641 gggggcacga tccacggcgc gaggacggcg agcgtgggga tgagcatcag ggccaggccg
     8701 ggagcgacgg cagcccggac cgtcgccggg agctgggtga aggccatgac gcacagcagc
     8761 gggacgcaca gcatcagggc catggccagg ccggtcagca gcctggcccg gctgcggcgg
     8821 gacgccaggg acccttccgt gtccttcatg agcgcgcccg gactcacgcg cccggcggcg
     8881 agcacactgg ccatggccga caggacggcg acggccccgg tggcgatgca cacgcccacg
     8941 gccgtgcccc acacctgacc cgaggacggg agggaggccg cttcggggaa gagctcccgg
     9001 tccacgagca gacgtcgccc cgcatgcgcc aggggcagca tgcccagtgc tcctacggcg
     9061 gctgcggcga ccgcgagccg cgccacgtcg cccgcgacca gacgacgcag ccgcccggcc
     9121 gtgaccccca gagcgcgcag aacggcgaac tgcccgagcc gctgccccgt ccacagcgag
     9181 acggtggagc cgatcaccac cacggcggac atcagcagca tggacagcag cagggagagc
     9241 aggctgcggg catcctcggc ctgggccgcc gccacggacc gggcggggaa gtccgcacga
     9301 cctgcagcga tcagcagcag ggtgcaggcg cccactcctg ccgccgtcac ggcgcaggcc
     9361 accgcggctc cggccaggcg gccggggtgg acgcgcgctt cggcgaggaa gaggcggcgc
     9421 atatgcatcg gtccggcaag ggggcgtgag ggtcgggacg ccacggacac tgtcttctgc
     9481 cctccacagt gcgtaagctg gaagtagcgc ggggaggcca cccacattgt ggcctccccg
     9541 cgcttgttta cggcgagcgg tgcagccgtt cttgaccgtg tgtcagtggc acccggcgat
     9601 gctgacggag ctccagggag cgtcgaggac agtctcctcg tcgagctcct gcagggcgag
     9661 aatgttctgc attccttgtc acctccttca ggggcacggt gggaaggggt tggtcccggt
     9721 ctgcgccggg gcggtgtctc agtggcagcc gccgaggctg atggtgctga tgggggcgtc
     9781 gacgatggtc tcctcgtcga gctcctgcag ggcgagaatg ttccgcatct ccggttcacc
     9841 tcccttctgc tgctcggccg ttcgctgtcg gggcggtgcc gcccgccggg ggcacctcac
     9901 gacgtcagtg gcacgcggcg acgctgacgg agctccaggg agcgtcgagg acggtctcct
     9961 cgtcgagctc ctgcagggcc aggatgttct gcatgtcttc tcacctccct tcgcgggctc
    10021 gcagggccgg gtggtccgga gcgtcgctcc cggccggcgg ccacccgcac gtggggtgag
    10081 cgccggggag cagacggctg ccggggttct tggcggcggc aagcgcgatc agcgcgcctg
    10141 cggagccggt cgccaggtcc gccgagagac ggaccagctg gtcgccgtgc aggacgtgtg
    10201 cgtcctcgta cggcgccaag tgcaaggtga gggcgcgcag atggctgtcc agctccgctt
    10261 gccaccgtcc cgtgcgggac agggcgtggg agagcaggta cgccagtccg gagcggccgc
    10321 tgaacagccc gccctgcagg acgacttcca ccgcgccggc ccgggcggca ccggccacga
    10381 tgccgtcggc accgagcgcg gccgcctgtg gcggggggag cgcgagggcg gccatcgcga
    10441 cgccggcgga gccttccgcg aggtacggca gcagccggct cgaactgtgc atctgcagca
    10501 tgccgtccac gtcacgggcg tgcgccaggt cgcagcgcac ggcgcgttcg gccgccgccg
    10561 cccattgcag gtctccggtc acttcggcgc agcgggcgaa gagcaccgcg ggccccgacc
    10621 agccgtgcat cagccccggc cggtccagca gtcccttggt ccccacgcgt tcgcgtaccc
    10681 gcagcgcgag ggcgagcgct tcgtccttca gcccgacgtc caggaagaga tgggcgatcc
    10741 cggacagtcc gctgaacagc cccggcgagg cgggcagggg gccggccagg aggcggtcga
    10801 cgaggttcgc gccctgcggg tgccccagcc ggtgcaggag ccatgcggcg cccgccatcc
    10861 cgtcgtagag gcccgccggt gtgtccaggg gagcccggcg caccgcaccg gccagccact
    10921 ccacgtcgtc gtcggagacc gccgttccgg ccgcgtgctg agcccacagc acgcccggcg
    10981 tgccgtacgc gagccccagt gcggcgcccg ggcggtgccc ggccacgtcg caggggtagc
    11041 gccggtcctt acggccggtg tcggccgagc gggcgatgcc gcgcagcaac gtgtccgtgg
    11101 acggcggcgt gaggggcggg ggtggcccgg cgagtgtgcc aggcggcggg aggtgcggac
    11161 gtaacgcagg tgacagagcc aggcgttcgg tcatctgccg gatgaactcc gccggcaggg
    11221 aggggaatga ggcccgggcc agctcgatca gggaggccac cgagcgcggg ttgcgcaagg
    11281 tcagcacggt gagggggacg agggccgcga gctgacagca gcccagggcg aacaggtccg
    11341 cctcccgggg atcggtgagg gcgggatcgg cgaagccggg cgcccccagt gcggcgctgc
    11401 ggaccgagcc gaccggggcg gcgagctcga agtccacgag cgccacggtg ccgtcgggcc
    11461 ggatcaggat gttgcggtgg tgcaggtctc cgaaggccac tccggcctcg tgcagcgccc
    11521 gtacgcattc cgcgacctgt tccatccgct cgacgacgga cgcggcgaat cgggtacggg
    11581 ccgcctcggt gggccggtgg cgggtgagcg ggtgctcgcg ccccagccag gcgctgacgg
    11641 tctcgccctc gacgtactcc atggcgaggt agtggtgttc ccaggcggtg aacgcgccgt
    11701 accggcgcgg gacgaacgga agatgcccga gtccgtccag gaccgcgtcc tcggtgcgca
    11761 gcctgtcgac ggcgtccatg ccgttggcgt ccaggccggc gtgcggacgg gcttccttga
    11821 ggacgacctg tctgccctcg ccgtcgtgcg cgaggtacac gcccccgccg ttggagaagt
    11881 ggagcgcctt gcggacctcg tagccgccga gagaggccga ctcgccgtcg gccgcaggcc
    11941 gctccgcgag gaagtccggg acgtcggccc acggggggag ggtgaagacc gggcggcggt
    12001 ggtcgggcac cagctgcccc tgaggggtgc gcagcgccgg tacgcgggtg ccgtccgcgc
    12061 gttcgcacca catcaggatg aaggctccgt aacgaaccga caccgggccg tcgttccagc
    12121 gccggtcgga cagcacgtag ggtccgggcc gcccgcgcat caccgtctcg agctcatcca
    12181 gagccctgcg cagctcgtct gtggtccggg gatagacggt cacggccttg ccgctcgcgg
    12241 cccggtgggc gtacttggtg ttcatggcgg cgacgatccg ccggctgcgc aggaacttcc
    12301 acggcagctc cagctgccgg cagacggtcc aggccgcctc tatggtctcc tcggcggcgc
    12361 cgggcgtcgc actcacgtgg atcttccagc cctgctccgg cagctttccg gtgtcaccgg
    12421 gcatcaggaa ttcccatacg ccctggtgtt cccgccgcca gccctgcggg agttcccgca
    12481 cggagaggga gaaaagagaa tccgagtcct ggcagcgctc cagcggctcg taccacgtgg
    12541 aatcggcgat cgtgtacgcc tcgtagctcg gatccattgt gccctccctc tgtgtgccgg
    12601 gcggcaggtt tcctgtgccg ccgtcgattc actgaaatgg gttcccggtc gccggagttc
    12661 tgcgaccggg gagtggaacg cttttccgtt ccgctgtgat aatcctgcgc ctgccggccg
    12721 ccggcccgca atcggcgtaa gttcacgctt aagcggagat ctgccttccg gccgcggcgg
    12781 ccgcgggctc ccgtctttcc cggccctgct gaaaggccgt atgacgcagc gtgggctcct
    12841 gacgcagcgt cggctccgcc gggcgccgtt ccgcggcctc gccgcgcagc aggaggcggc
    12901 cgagatgagt gacggtgtgg atggcatgac tgcggctgcg gcgcgtcgag accagcccgg
    12961 cggcccgcag gacggccatg tgctggctca ccgcggccgc ggacacgccc agccgttggg
    13021 ccagctcggc gttggtacag ccctcccgta gcgcacggag cgcggccgct ctggtccgcc
    13081 cgacgagggc ggcgagactg tccggcccgc aatgcggacc gcccgcccgc ccgccgccca
    13141 gcaactgcgt gagctcctcc ggtccgggac ggtcgggaaa caccagcacg ggcgcgcggt
    13201 gcactccctg ccgggcaggc gggagcacat gtcccgcgcg ctgcaggaac aaggacggag
    13261 ccagcaccag cccggtgccg tccagctcca tctccccgga gcgcacgccc gcgatctcca
    13321 gggcgggcgg gttccagcgg attcccggac ccagcgagtc cagcagcact tccacgccgt
    13381 cgcggcacat gatgtcccgc aaggtgtcgc ggacctcgcc cagataggca cggatccgcc
    13441 cccaatgcgg tgcgacggcc atctgctgga agtcctggac cgcggaacgc agggacaccg
    13501 ggggagcccc tgcgccgttc ggcggcacca cggtgtggcg tcccccgccg gtacacaatt
    13561 ccagcagctt tcccggaccg cccggaaccc ggatcagcgt cgcgacccgc tccattctcg
    13621 gatcggcccg ccggccgccc ccgcacactc cttgccgcca tgcgcgaaat ccctctcccc
    13681 ggccctcccg gagcgcctcc agggcgaaca aggcctccat ggagaggaga tccgcttcgc
    13741 tgatcctcac gcgtgccagg tcgtcgaccg agaaaatgat gcgatacacg agagcggccc
    13801 ccgcagcgat cggctccacc ctgagtgcgc ggcaacgtcc tggtgccggc ggacggagcg
    13861 ttggccaatg ctgatgtgca tggttcccgg ctcccgcgcc gcacggggga tggataatcg
    13921 agtccgtcac ctcaggaggc ccgtcaggag gcccgtcagg aggtccgtca cctcaggaga
    13981 gacgggtgac gaagggcgcg ggctaacctc ctgacatgga ggcactcgtc gtacggttgt
    14041 cgcaactcga tgcggaagca gaaggcgcga tccgcgtcgt catgttctac gacacgctga
    14101 tgcgccgccg ggtggacctt ccggcgctcg cccgggcctc ggcaagcctg gccgagtgcg
    14161 tggccgggat acggctcttc ggcacggggc gcttgatccg cataggaccc gacggccgtg
    14221 aggctcctgc cgctgaagct ctcgcccctg aagcacttgt gtccacgacg gtgccgatca
    14281 cgctggacga cgaagcgatc ggcgcggtgt ggctggaacg ttccggcccg cccggcccgt
    14341 tcgacgacct gctgctggac cggctcgccc tcgccgtctc cgcggccgtc gagcgacacg
    14401 gcccggcctt caccaccatg gccgaccccg ccctggtgga gctcgtgatc agccccggca
    14461 gcgacgaggc cgccagagcc cgggcgctgc ggctcatggg cttctccgcc ggcctgccgg
    14521 tccgcgtcgt cgccgtacgg tcggagatcc ccctcgacca ggtgggcgga tcgatctgcg
    14581 cggcccgtcc ggtgaaggcg gcacaggtgg cggacgtggg cgtcatcctg gccgcgacgg
    14641 tcgccgccga ccggcttccg gcaggcgtac gcgcgggcat cggcatcgcc ggcaacccgg
    14701 tcctgtcatg gcaacaggcc cgcaccgccc tccgcttcac cagcccgcgc gaacccgtcg
    14761 tgcactacgc cggactgggc gcactggccc tgctcgccga aatacctcag gacgtctcgc
    14821 gggacaacgc cgacgtggcc gcgatcgccc gcatggccgg cagcccggac gatctggaga
    14881 ccctcgacgc ctactgcacc accggatccc tgcgccgggc ggccgatctc ctgcacctgc
    14941 accacagcag cgtggcccgg cgcctcgaac acatcgggac gaccctgggg atcggcctca
    15001 ccgagcccgc cggactggtc cgcgccaggc tcgccctcac cgcatggcgg ctgctcgacg
    15061 actgacaccc ggctccccgc gcgcaccgga cgatcccggc acgcaccgga cgagctgccc
    15121 cttcgaggtg acagcgcggg cccgctgacg gggcgtccgg aaagagccac ccgggcgtgc
    15181 aagggcgaaa ccggcgtggt gcaggatgac ttctcgtgcc gggcccttcc gtggcccggc
    15241 acgagcacga tccgtcggga cagacaggta caaggtgacg aatcattact ccgggcttcc
    15301 ggcgatggcg tacgacgccc ccgctccctc ctccgcaggg cggggaggac accggtgaac
    15361 cgggatctga cactgcatga catcatcgtc gccggcatcg ccgtggccgc aggcctggcc
    15421 ctcgccctcg tgctgcgggt gatccttcgc tggctcggcg tacgcgccct caggacccgc
    15481 tggcagggcg acgacatcat cgtcgacgtg ctgcgcacgc tcgcaccggg gtgcgcggtc
    15541 gcggccggcg tggcggcggc cgccgcagcc ctcccgctga ccagccaagt cggacacatc
    15601 gtcgacgagg tggtgatggc ctgcatcgtc ctgatcgcga cgttcaccgc cgcccgggtg
    15661 atcagcggtc tggtgcgctc catggcgcag tccagatcgg gcgtggccgg atcggccacg
    15721 atattcgtga acatcacgcg catcgcggtg ctggccatgg gcttcctcgt catgctggaa
    15781 accctcggca tatccatcgc cccgctgctc acggccctcg gcgtcggcgg cctggcgatc
    15841 gccctggccc tgaaggacac cctggccaac ctcttcgcgg gtgtgcacat cctcgtctcc
    15901 aagacggtcc agcccggcga ctacatacgg ctcagcagcg gcgaggaggg ctacgtccgc
    15961 gacatcaact ggcgcatcac ggtggtggag cagctcaaca acaacctggt catcatcccg
    16021 aacgccaagc tcgccggcac gaacatgacc aatttcagcc ggcccgaaca gaagctgtcc
    16081 gtcacggtgc aggtgggcgt cgcctacgac agcgacctgg agcgcgtcga gcagatcacc
    16141 gtcgatgtcg ccgagagcgt gatgaaggat gtcgcgggcg ccgtacccga ccatgagtcg
    16201 gccgtccggt tccacacgtt cggggactcc cggatcggct tcacggtgat cctcggagtc
    16261 ggcgagttca gcgacaagta ccgcatcaag cacgaattca tcaagcgcct gcaccagcgc
    16321 taccgcgccg agggcatccg gatccccgct ccggcgcgga ccgtctccct gcaacagacc
    16381 ggggagcccg ccatcccgca tccccgcgcg tccctcgacc aggcttcgta ggcccgggtc
    16441 ccgggggctc cggaattcgc tgacgctgtg cgtgtgagtg ctcgctgtgt gtcgtctcgg
    16501 gggctgctcc gtgtgagggc gcccatgagg cctgggtcac ttacgaatgc cgttagtgga
    16561 attcggcggc ggaattaagg cgccgggtcg ggttttcgtg tgcgggcgat gaatgggcgt
    16621 agtaggtcac aggccttgtc agggttttcg gtggtggcta aaacttgtgg agtcgctggg
    16681 cgccgtcgac cgagaaacgg cgcttgtttt tgcgccgctt cagggccggg cgacatcccg
    16741 cgatcggaag gttcagctca gttatgcgct ccaccgtctc cgcttatgtt cgtctgccga
    16801 agattcacaa gttctctgtt gccggtattg ccgcgaccgg tgccgctgtc gtcgcggtgg
    16861 ccgttgctcc gcatgctttc gctgctgatg cggctccggc tgctgcggtg aagccggtgg
    16921 cgtggagtgc tgaggctttc ggtgagacgc agcaggccga cctggagaag catgctgacg
    16981 ccgccgccaa ggccggcgcg gacgctgccg ccgaggcgaa ggcgggcaag gagcgtgcgg
    17041 agaagcaggc cgccaaccgt tccaccgagc gcaaggccat agccgccaag ccggccgctg
    17101 cggcgaagcc cgctgccaag cctgctgccc cggccaagcc cgctgccaag ccggctgctc
    17161 cggtgaagaa ggcctacgcg gatgatcttg agggctggat cggtcagtcg ctggacattc
    17221 tgcgggcga
//
