LOCUS       NZ_BMUT01000015        11317 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces hiroshimensis strain JCM 4586 sequence15, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUT01000015
VERSION     NZ_BMUT01000015.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces hiroshimensis
  ORGANISM  Streptomyces hiroshimensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 170201)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 90X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650335.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:32:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,830
            CDSs (total)                      :: 6,745
            Genes (coding)                    :: 6,620
            CDSs (with protein)               :: 6,620
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 25 of 125
            Pseudo Genes (incomplete)         :: 107 of 125
            Pseudo Genes (internal stop)      :: 4 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:07:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 51746
            Orig. end                         :: 63063
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUT01000015.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11317
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11317
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11317
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6317](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6317
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(236..253)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 13.98"
                     /tool="antismash"
     gene            421..1959
                     /locus_tag="IE241_RS28275"
                     /old_locus_tag="GCM10010324_57760"
     CDS             421..1959
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1066: alpha/beta hydrolase domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003986164.1"
                     /locus_tag="IE241_RS28275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_57760"
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="WP_190024695.1"
                     /transl_table=11
                     /translation="MTVAASEPSGSRPEVRAAAGALRGSREAGVAVFRGIPFAEPPVGP
                     LRFAAPRPVRRWDGVRTAVSYGPPPPQGGHFGMDALARETAGDDWLTVNVWSPEPVPGA
                     GLPVMVWIQGGAYVIGMSGLPEYDGGRLAREGGVVVVTFNYRVGLEGFGQIEGAPANRG
                     LLDQIAALEWVRDNIRAFGGDPDRVTVFGQSAGAGSVAALLAMPRAAGLFVRAVAQSVP
                     GTFFSPELAADIAAACAAELGLKPTVAGLAAVTPERLSAAGDAVGAETARWADRWGPIA
                     HKSIPFAPVVDGDVLPSAPWQALAGGAARDVALLVGHTRHEQRLLTALEGLLGQVTQEQ
                     AEAALRVFGPGPDGARRYRDGFPDAGPDELYELVHSDWLFRMPSLHLAEARAAADGRTH
                     VYELTWDAPGMGGVLGACHGLDVPLVFGNLDRGQPAALIGEGPPPEAEELSARMRAAWT
                     AFAAHGDPGWPPYDSGRRLVQLFGARPDVAAYPEETSRLIWQDHTFPVLPLTGP"
     gene            complement(2038..3927)
                     /locus_tag="IE241_RS28280"
                     /old_locus_tag="GCM10010324_57770"
     CDS             complement(2038..3927)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547891.1"
                     /locus_tag="IE241_RS28280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_57770"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_190024696.1"
                     /transl_table=11
                     /translation="MTTNTAAPRIPTPAAQPELDLGWDDPHFHRVASASLRQMAGRLPA
                     VLGRAARLGWAADRAAMVQILLAQCLAAVAAAVALAATARVMTGLFGPGTAAEHLHAAL
                     PALTLAAAAAAVRACAEATAQYAALRLAPEVTALADESILADTPEVELTAYDRPGFNDA
                     LQAAGTGAQATQDLITDAQVLLSAVAQLIAAAAVLTTLNPLLLPLLALAVLPKGAAAVA
                     AARIAHRAAHTSLADNRLRYILRRYSTERHMAAEIRAGTMAPYLRQWYAHLTARIRTRH
                     RAAAPRVLLVTLCGAAGSGIMLALTWLTVGALVLGGAMGLAVAATAVVAVRASTAALTS
                     LVMAGARLFRTSLYLQDWQAFLDLAKELRAVRGPLRLAADGPREIRAHEVSFRHPGAPS
                     GRPALDRVSFTLRSGELVALVGENGSGKTTLSRLLTGLYLPTAGRVTWDGTDLADTDPQ
                     DVWSRVGLVPQDYTRWPMAARENITLGQPRGGDDLVRAAARAAGADSVLDGLPDGMDTL
                     LAKSFWGGHDLSGGQWQRFAVARAFYREAAVLVLDEPTSAMDPRAEHTVISRFKELAAG
                     RAAVFVTHNLTNTRIADRIVVLAGGRVVEEGTFEELSVGGGLFAELYKLSQDR"
     misc_feature    4054..4068
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.68"
                     /tool="antismash"
     gene            complement(4101..4817)
                     /locus_tag="IE241_RS28285"
                     /old_locus_tag="GCM10010324_57780"
     CDS             complement(4101..4817)
                     /EC_number="2.8.2.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (extras)
                     Sulfotransfer_4: Sulfotransferase domain"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF036807.5"
                     /locus_tag="IE241_RS28285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_57780"
                     /product="sulfotransferase family protein"
                     /protein_id="WP_190024697.1"
                     /transl_table=11
                     /translation="MTPERVVRPGSAPVPGPVPAPVPGPVPRPGPPPRAAARPKIFGIG
                     LSRTGTLSLTVALRTLGFDVAHYPADPDTYAALLAGTARFPLLDHRDGITDITVVPYYQ
                     DLDRAWPGAKFILTVREEEGWLTSCRTHWKPSIDGKAGLDEVYPQLQRFLRAAVYASHE
                     FHAERFRTVARRHTAEVLRHFAGRPGDLLVLDITAGQGYERLAPFLGVPVPGEPFPHTN
                     HTNVYARVPVDGERAR"
     gene            5001..6317
                     /locus_tag="IE241_RS28290"
                     /old_locus_tag="GCM10010324_57790"
     CDS             5001..6317
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF014661.5"
                     /locus_tag="IE241_RS28290"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_57790"
                     /product="YcaO-like family protein"
                     /protein_id="WP_190024698.1"
                     /sec_met_domain="YcaO (E-value: 2.4e-26, bitscore: 86.4,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPHPDAPGDAPSPDGSGRAQRWRPEVFAPFAESPGVVFARTAA
                     RSPQFGRTSASGADRVLVGSAAGDDRGDVALRARGELLERMGNVLAGRAAESAARWVGD
                     YDGWRRRGVPALDPAELGDGTEGAAGSAGTAGRAVRQLWVPARSVLTGDELLVPAGEVF
                     LQHRPPPGCGTTSGAGSTGLGAHPDRTAAAAHAARELLERDAIRRSWYGTGAPPPRAVP
                     AEDLLPGPVRTLLTALGLRATLLMLPSPAGTECAVVCVHGGADGRPGQSFGARCAAAGD
                     SAATAAGAAYEALMVRWSMTSPVALRTWEGWTGRTPPRTALEHALWVFHGQDALGHWTG
                     RERGTAGADAPGDGQGGGPAGAAGAPNGRGARERRSPEPSGGLAALAAHTGGDVLLVDT
                     DTPGIRAEGVCVVRVIAPGARPLPADDSRIPGALPHPFG"
     gene            6358..7197
                     /locus_tag="IE241_RS28295"
                     /old_locus_tag="GCM10010324_57800"
     CDS             6358..7197
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140354.1"
                     /locus_tag="IE241_RS28295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_57800"
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="WP_190024699.1"
                     /transl_table=11
                     /translation="MTSSGSPDYTAFAARYDSWFAASGATAATVAALAGLAEAAGPGPA
                     LELGIGTGRIAVPLAARGVEVHGIDASPAMVAQLRAKPGGEHIPVTMGDFGTAGTGDGA
                     AAGAEAVTEAATDAGSDAAAGADAPGTYALVYVVSGTFAELPSQEAQVDCFAGAARRLR
                     RGGMFVLDAHVPEALAEDRSTGGQVLPTEGSELVLRFRETDRAAQRYRSHYIVLDKTPE
                     GLGMHRTTVSFRYAAPGELDLMARLAGLRLRERWGDWTGAPFTSSSAYHVSVYERPE"
     gene            7220..8059
                     /locus_tag="IE241_RS28300"
                     /old_locus_tag="GCM10010324_57810"
     CDS             7220..8059
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /GO_function="GO:0008425 -
                     2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0102208 - 2-polyprenyl-6-hydroxyphenol
                     methylase activity [Evidence IEA]"
                     /GO_process="GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1089: methyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF019847.5"
                     /locus_tag="IE241_RS28300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_57810"
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="WP_190024700.1"
                     /transl_table=11
                     /translation="MDPEAYMAENLRQWEAWTPLKAASAFYGLDDFRRGGMRIRRLEVE
                     EVGDVSGLSLLHLQSHVGLDTLSWARLGARVTGVDFSAEGTATARSLARDVAPSARFLC
                     ANVYELPGHLDETFDLVYTSHGVLGWLPDLRRWAAVVARFTAPGGRFYLFESHPTAWLF
                     DNRTDSRELTVRYPYFGAGEPLRWRYRSPLAVPEAEADGWEFSWGHALGDIVNALLEAG
                     LVIEFLHEWPFVAWPMFPFLEKRDDGWWHLPEDVPAVPLSFSLRARKPAQGAGRDAL"
     gene            8436..9416
                     /locus_tag="IE241_RS28305"
                     /old_locus_tag="GCM10010324_57820"
     CDS             8436..9416
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:TIGR04267.2"
                     /locus_tag="IE241_RS28305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_57820"
                     /product="HEXXH motif-containing putative peptide
                     modification protein"
                     /protein_id="WP_190024701.1"
                     /transl_table=11
                     /translation="MDVEPDLTAAHADQDALLRLLAAVAQGAGLPPLPLHPAAVEAAHR
                     AQAAARHANGSPPPQAPAPAPAPAPANGWAGPPAPAATGPAAWPVELAGPADHPHLLRS
                     LRRALAQLPPRPGADGQPVTAALGPWRTADREALEAAVRLLQEAWPAMLDELRAVVRQV
                     ALLDGMAIDGFTDFTVHGAVLVNRRRLSAARSGLPGPARLAEALVHEGTHNRCNVAGVR
                     APFLRPAADDETGPLTTPLRSDPRPLSGLFQQTVVLARCVLLHRRTADGGALSAAAEAA
                     VRARHDRLLTDLDAAVSSLSAHRAGLTEHGVDMLQQCAAVARSAV"
     gene            complement(9506..10471)
                     /locus_tag="IE241_RS28310"
                     /old_locus_tag="GCM10010324_57830"
     CDS             complement(9506..10471)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF017532.5"
                     /locus_tag="IE241_RS28310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_57830"
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="WP_190024702.1"
                     /sec_met_domain="PhyH (E-value: 4.6e-09, bitscore: 30.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTRPADGPAAGSVDGWAGSAAGSVGAPAALHSVEPGPYGIPVSLR
                     HVLDGALVAVKGGLRHIGALETIRREAARAVAAALPAAEAAQVSGALENAHRHADTGQI
                     AAVRTDLETRLRPFSRTVLHDVARALEPQGARLYLSGHLGIRIMPPERSVGAGEEELAG
                     LEGFLTPRDAHVDSWFNTAVNSVNLWMAVGPVRSGNGLLFYPDAYRRPLRHDGHTLVPG
                     QEVGAPVDVELDPGDILLFAGDHLHASQPNSTDETRFVITKRLCVGVPRYPSGATGWVP
                     YEDPRLWGTRWERWATWRSRLTPGGVRETLRTARHRRSGS"
ORIGIN
        1 tgtggccgtg cgggcggtga cgcgcagggt ggcggtgtcg acggtggcgg tgccgccgcg
       61 gacggggatg tccaccgact ggccgtgcac gcggacggcc tggccggtcc cggctgcggc
      121 gtgggcgggc tcggcgaggg tgatgcacgg gagggtggcc gccagcacga cggcggcggc
      181 gcgggcggtg cggcaggagg tgaagaagtg catggggatg tgatagcgaa cggaggtaag
      241 agggttttaa gaactcctcg ttggactcct gctgcatgcc tcgttgcgct ctgttccgcg
      301 ggtccgggcc caccgtaaat gactggcgcg catcgtgaca tagcgggatg ctccggagag
      361 ccggggccga ccgacgcgaa ccctgcggtc gtgctccacc cgcctggaga gggagccgtg
      421 atgaccgttg ccgcatcaga gccgtccggg tcccggcccg aagtccgcgc ggcggccggg
      481 gcgctgcgcg gcagccggga ggcgggggtg gcggtcttcc gcggcatccc gttcgccgag
      541 ccgccggtcg gtccgctgcg cttcgccgcg ccccggccgg tacggcggtg ggacggagtg
      601 cgcacagccg tgtcgtacgg gccgccgccc cctcagggcg gccacttcgg catggacgcg
      661 ctggcccggg aaacggcggg cgacgactgg ctgacggtca acgtctggtc gccggagccg
      721 gtcccgggcg cagggctgcc ggtgatggtg tggatccagg gcggcgcgta cgtgatcggc
      781 atgtccggcc ttcccgagta cgacggcggc cgcctggcgc gggagggcgg cgtcgtcgtg
      841 gtgacgttca actaccgcgt ggggcttgag ggtttcgggc agatcgaggg agcgccggcc
      901 aaccgcggcc tgctcgacca gatcgccgcc ctggagtggg tgcgggacaa catccgggcc
      961 ttcggcggcg acccggaccg cgtcacggtc ttcggccagt cggcgggcgc cggatcggtc
     1021 gccgcgctgc tggcgatgcc gcgcgcggcc gggctcttcg tccgggccgt cgcgcagagc
     1081 gttccgggca cgttcttctc gccggagctc gccgccgaca tcgccgccgc ctgtgccgcc
     1141 gagctggggc tgaagcccac ggtggccggc ctggccgcgg tgaccccgga acggctgtcc
     1201 gccgccggtg acgcggtcgg cgccgagacg gcccggtggg cggatcgctg ggggccgatc
     1261 gcgcacaagt cgatcccgtt cgcgccggtc gtcgacggcg atgtgctgcc gtccgctccc
     1321 tggcaggccc tggccggtgg cgccgcccgg gacgtcgcac tcctcgtcgg ccacacccgc
     1381 cacgaacagc ggctgctcac cgcgctcgaa ggcctgctgg ggcaggtgac acaggagcag
     1441 gcggaggccg ccctgcgcgt cttcggcccc ggcccggacg gcgcacgccg ctaccgcgac
     1501 ggctttccgg acgccggacc ggacgagctg tacgaactgg tccactccga ctggctgttc
     1561 cgcatgccga gcctccacct cgccgaggcg cgggccgcgg ccgacggccg cacccacgtc
     1621 tacgagctga cctgggacgc cccgggcatg ggcggtgttc tcggcgcctg ccacggcctc
     1681 gacgtgccgc tcgtcttcgg caacctggac cgcggccagc ctgccgcgct gatcggcgaa
     1741 ggcccgcccc cggaggcgga ggagctgtcc gcccgcatgc gggccgcctg gacggcgttc
     1801 gccgcccacg gcgaccccgg ctggcccccg tacgactccg gccggcgcct cgtgcagctc
     1861 ttcggcgcgc ggccggacgt cgccgcctac ccggaggaga cgtcccgcct gatctggcag
     1921 gaccacacct ttcccgtcct gccgctgacc ggcccgtagc gcaggccggc cgtagctcag
     1981 accggccccg tagtgcaggc cggctcgcgc cgagcgtccg tcaccgccgg gcgcccgtca
     2041 ccggtcctgg gacagcttgt acagctccgc gaagagcccg cccccgacgg atagttcctc
     2101 gaaggtgccc tcctccacga cgcggccgcc ggcgaggacg acgatgcggt cggcgatgcg
     2161 ggtgttggtc aggttgtgcg tgacgaacac cgcggcacgg cccgcggcga gctccttgaa
     2221 gcggctgatg acggtgtgct cggcgcgcgg gtccatcgcg gacgtgggct cgtcgaggac
     2281 gaggacggcc gcctcgcggt agaaggcgcg cgccacggcg aagcgctgcc actggccgcc
     2341 ggagaggtcg tggccgcccc agaacgactt ggcgaggagg gtgtccatgc cgtccgggag
     2401 gccgtcgagg acgctgtcgg cgcccgcggc gcgggccgcc gcgcgcacca ggtcgtcgcc
     2461 gccgcggggc tggcccaggg tgatgttctc gcgggccgcc atcggccagc gcgtgtagtc
     2521 ctgcgggacc agtccgacgc gggaccagac gtcctgcggg tcggtgtcgg cgaggtcggt
     2581 gccgtcccag gtgacccgcc ccgcggtggg caggtacagc cccgtcagca gccgggagag
     2641 ggtggtcttg ccggagccgt tctccccgac cagtgcgacg agttcgcccg agcgcagggt
     2701 gaagctcacg cggtccaggg cgggtctgcc ggacggggcg ccggggtggc ggaaggagac
     2761 ctcgtgggcg cggatctccc ggggcccgtc ggctgcgagc cgcagcggac cgcgtacggc
     2821 cctcagctcc ttggccaggt ccaggaaggc ctgccagtcc tggaggtaga gcgaggtgcg
     2881 gaagagccgg gcccccgcca tgaccaggga cgtcagggcg gccgtggagg cccgtacggc
     2941 gacgaccgcg gtggcggcga ccgccaggcc catggcgccg ccgaggacca gcgcgccgac
     3001 ggtcagccag gtgagcgcga gcatgatgcc ggagcccgcc gcgccgcaga gcgtcaccag
     3061 cagcacgcgc ggggcggccg cgcggtgccg ggtgcggatc cgggcggtga ggtgcgcgta
     3121 ccactggcgc aggtacgggg ccatggtgcc ggcccggatc tcggcggcca tgtgccgttc
     3181 ggtgctgtag cggcgcagga tgtagcgcag gcggttgtcg gcgaggctgg tgtgggcggc
     3241 acggtgggcg atgcgggccg cggcgacggc ggcggctccc ttgggcagca cggcgagcgc
     3301 cagcaggggc agcagcaggg ggttcagggt ggtgagcacc gccgccgcgg cgatcagctg
     3361 ggcgacggcg gagagcagca cctgggcgtc ggtgatcagg tcctgggtgg cctgggcgcc
     3421 cgtgccggcg gcctgcaggg cgtcgttgaa gccgggccgg tcgtaggcgg tcagctcgac
     3481 ctcgggggtg tcggccagga tgctctcgtc ggccagggcg gtgacctcgg gggccagccg
     3541 gagggccgcg tactgggctg tggcttccgc gcaggcgcgc acggctgcgg ccgcggcggc
     3601 gagggtgagc gcgggcagcg cggcgtgcag gtgctccgcg gccgtgcccg ggccgaagag
     3661 tccggtcatg acgcgggcgg tggcggcgag cgcgaccgcc gcggccacgg ccgccagaca
     3721 ctgggcgagc agtatctgca ccatggccgc ccggtccgcg gcccacccca gccgggccgc
     3781 ccggccgagg acggccggca gccggcccgc catctgccgc aggctcgcgg aggccacgcg
     3841 gtggaagtgc gggtcgtccc agccgaggtc gagctccggc tgcgcggcgg gggtggggat
     3901 gcgcggggcg gcggtgttcg tcgtcaccgg gtcatggtgt cgtcacaccg ggtgccgcgt
     3961 ggcggaactc tccggtcggt ggccctttag gaggaagagg aggagaggag gaagggacgg
     4021 tgcaagccag ttcgccggcg tccggaaacg gccggatgtc ttcggcgacg tggacgcggt
     4081 ccggacggat gtcctcgcgc tcaccgcgct ctctcaccgt cgaccggcac gcgcgcgtag
     4141 acgttggtgt ggttggtgtg cgggaaaggc tcgccgggga cggggacgcc gaggaagggc
     4201 gccagccgct cgtagccctg cccggcggtg atgtcgagga ccagcaggtc gccggggcga
     4261 ccggcgaagt ggcggagcac ctcggcggtg tggcggcggg cgaccgtacg gaaccgctcg
     4321 gcgtggaact cgtggctcgc gtagacggcg gcgcgcagga agcgctgcag ctgcggatag
     4381 acctcgtcca ggccggcctt gccgtcgatc gagggtttcc agtgggtgcg acaggaggtc
     4441 agccagccct cctcctcgcg gacggtgagg atgaacttgg ctcccggcca ggcccggtcg
     4501 aggtcctggt agtacggcac gacggtgatg tcggtgatgc cgtcgcggtg gtcgagcagc
     4561 gggaagcggg cggtgccggc gaggagcgcc gcgtaggtgt ccgggtcggc cgggtagtgg
     4621 gccacgtcga agccgagcgt gcgcagggcg acggtcagcg acagggtgcc ggtgcgcgac
     4681 aggccgatgc cgaagatctt cggccgggcg gcggcgcggg gcggcgggcc ggggcggggg
     4741 acgggaccgg gtacgggtgc gggtacagga ccgggtacgg gtgcggatcc ggggcggact
     4801 acgcgttcgg gtgtcatgtc gagcggcctc ctcgcggggg ggcgggacgg tacggcgccc
     4861 ggcggcccgc gcgccgtggt caccgtgccg gatcacacgg aattgtcaac ggcgcgccga
     4921 acggccggaa ttcactgccg gggcggcggt tgcgccgggt gccgttgtgc tgacgctgcg
     4981 cctttggtcc aatcgaggcg atgaccacac cgcacccgga cgccccggga gacgcgccgt
     5041 ccccggacgg ctccggccgt gcgcagcgct ggcggcccga ggtgttcgcc ccgttcgccg
     5101 agagcccggg cgtggtcttc gcccgcacgg ccgcgcggtc accgcagttc ggccggacga
     5161 gcgcgagcgg cgcggaccgg gtcctcgtcg gcagcgccgc cggcgacgac cgcggcgacg
     5221 tcgcgctgcg ggcccggggc gagttgctgg agcgcatggg caacgtgctg gcggggcggg
     5281 ccgccgagag cgcggcgcgc tgggtgggcg actacgacgg ctggcggcgg cgcggggtgc
     5341 ccgccctgga cccggcggag ctcggcgacg gcacggaggg ggcggccggg tcggctggga
     5401 cggccgggcg ggcggtgcgc cagctgtggg ttccggcccg ttccgtgctc accggcgacg
     5461 aactgctggt cccggcgggc gaggtgttcc tccagcaccg cccgccgccc ggctgcggca
     5521 cgacgtcggg ggcgggctcg acgggcctgg gcgcccaccc ggaccggacg gcggcggccg
     5581 ctcacgcggc ccgtgagctc ctcgaacgcg atgcgatccg gcgcagctgg tacggaaccg
     5641 gcgcccctcc gccgcgcgcc gtccccgcgg aagacctgct gccagggccc gtacggacgc
     5701 tgctcaccgc cctggggctg cgcgccacgc tcctgatgct tccttccccg gccggcacgg
     5761 agtgcgccgt ggtctgcgtc cacggcggcg cggacggccg ccccggccag agcttcggcg
     5821 cccgctgcgc ggcggcgggc gactccgcgg cgacggcggc cggggccgcc tacgaggcgc
     5881 tgatggtgcg ctggagcatg accagccccg tggccctgcg cacctgggag ggctggaccg
     5941 gccgcacccc gccccgcacg gctctggaac acgccctgtg ggtcttccac gggcaggacg
     6001 cgctggggca ctggacgggg cgggaacggg gcacagcggg ggcggacgcg cccggcgacg
     6061 ggcagggcgg cggcccggcg ggcgcggccg gagccccgaa cgggcggggc gcccgcgaga
     6121 ggcggtcgcc ggagccgtcc ggcggactcg ccgcgctcgc cgcgcacacc ggcggcgacg
     6181 tgctgctcgt ggacaccgac acccccggca tccgggcgga aggcgtctgc gtggtgcggg
     6241 tgatcgcccc cggcgcccgt cccctgcccg ccgacgacag ccggataccc ggcgcgctcc
     6301 cccatccctt cggctgacgg cccacaaggc cggccgaccg cgagaaaggg ctcgacgatg
     6361 acctcatccg gctcgccgga ctacaccgcc ttcgccgccc gctacgactc ctggttcgcc
     6421 gcgtccggcg ccaccgcggc caccgtggcg gccctcgccg ggctcgccga ggcggccggg
     6481 ccggggccgg ccctggagct gggcatcggc accggccgga tcgcggtgcc gctcgcggcc
     6541 cgcggcgtgg aggtgcacgg catcgacgcc tccccggcca tggtcgccca gctgcgcgcc
     6601 aagcccggcg gcgagcacat cccggtgacg atgggggact tcggcacggc cgggaccggc
     6661 gacggcgccg ccgccggagc cgaagccgtc accgaagccg caaccgacgc cggctccgac
     6721 gccgccgccg gagccgacgc cccgggtacg tacgcgctcg tctacgtcgt cagcggcacg
     6781 ttcgccgaac tgcccagcca ggaagcccag gtggactgct tcgcgggcgc ggcccggcgg
     6841 ctgcggcgcg gcgggatgtt cgtcctggac gcgcacgtac ccgaggccct cgccgaagac
     6901 cggtccacgg gcggccaggt gctgccgacc gagggcagcg agctcgtcct gcggttccgg
     6961 gagaccgacc gggcggcgca gcgctaccgc tcccactaca tcgtcctcga caagaccccg
     7021 gagggcctcg gcatgcaccg tacgacggtg tcgttccggt acgcggcgcc gggcgagctg
     7081 gacctgatgg cgcggctggc gggcctgcgg ctgcgcgagc gctgggggga ctggacgggc
     7141 gcgccgttca cgtcgtcgag cgcgtaccac gtctccgtct acgagcgccc ggagtgaacc
     7201 gggggaaggg agggcggcca tggaccccga ggcgtacatg gcggagaacc tgcggcagtg
     7261 ggaggcgtgg acgcccctga aggccgcctc cgccttctac ggcctggacg acttccgccg
     7321 cggcggcatg cggatccgcc gcctggaggt ggaggaggtg ggcgacgtgt cgggcctgtc
     7381 cctgctgcac ctgcagagcc acgtggggct ggacaccctc tcctgggcgc ggctcggggc
     7441 gcgcgtgacc ggcgtcgact tctccgcgga agggacggcg accgcgcgct ccctggcccg
     7501 cgatgtcgct ccgtcggccc gcttcctctg tgccaacgtc tacgagctgc ccgggcactt
     7561 ggacgagacc ttcgacctcg tctacacctc gcacggggtg ctcggctggc tgccggacct
     7621 gcgccgctgg gcggcggtgg tcgcgcggtt cacggcgccg ggcggccggt tctacctctt
     7681 cgagtcccac cccacggcct ggctcttcga caaccgcacg gacagccggg aactcactgt
     7741 gcgctacccg tacttcggcg ccggggagcc gttgcgctgg cgctaccgca gcccgctggc
     7801 ggtgccggag gccgaggcgg acggctggga gttctcctgg gggcacgccc tgggggacat
     7861 agtgaacgcg ctgctggagg cggggctcgt catcgagttc ctgcacgagt ggccgttcgt
     7921 ggcctggccg atgttcccct tcctggagaa gcgcgacgac ggctggtggc accttcccga
     7981 ggacgtgccc gccgttccgc tttcgttctc gctgcgggcc cgtaaacccg cgcagggggc
     8041 cgggagggat gccctttgag ccacataggc cgtggatacg cgggaatcgt tggccgacgg
     8101 acgacggcga gcggcacgga acggcctgct ccaccatggc gatcactgca ttccgggggc
     8161 accatgtcag ccgggtgcgc cggtgttccg acgcctcgtc gcacgcatga cccctcaccg
     8221 ccgaaggagc ggatatgccc caggacatcg tagaagtcca ggaagtcccg gccgaggagc
     8281 tccctgagct cgccctcatg atcgacatac cgttcgagga gtagcgcggc cgcactgcgc
     8341 ggaccgcact ccggcggccg accgggcagg gctccggtcg gccgctcctt cccggcagcg
     8401 accgcagcgg ggcggggata aggcaggagg actgcgtgga cgtcgagccg gacctcacgg
     8461 cggcgcacgc cgaccaggac gcgctgctgc ggctgttggc ggccgtggcg cagggtgcgg
     8521 ggctgccgcc gctccccctg cacccggccg ccgtggaggc cgcgcaccgg gcgcaggcgg
     8581 cggcgcggca cgcgaacggt tcacctccgc cacaggcacc ggcaccggca ccggcaccgg
     8641 caccggcgaa cgggtgggcc gggccgcccg cgccggccgc gaccgggccc gccgcgtggc
     8701 cggtcgagct cgccggcccg gcggaccacc cccatctgct gcgcagcctg cgccgcgccc
     8761 tggcccagct gccgccccgg cccggggcgg acgggcagcc ggtcacggcc gcgctcggcc
     8821 cctggcgtac cgccgaccgg gaggccctgg aggcggcggt acggctgctt caggaggcat
     8881 ggcccgcgat gctggacgaa ctccgcgccg tggtccgcca ggtggccctc ctggacggca
     8941 tggcgatcga cggattcacc gacttcaccg tccacggcgc ggtcctggtc aaccgcaggc
     9001 ggctctcggc ggcccgctcc ggcctgcccg gcccggcccg gctggccgag gccctggtcc
     9061 acgagggcac gcacaaccgc tgcaacgtgg caggggtgcg ggcgccgttc ctgcgccccg
     9121 cggcggacga cgagacgggc cctctgacca cacccctgcg atccgatccg cgcccgctca
     9181 gcgggctgtt ccagcagacg gtggtgctgg cccggtgcgt cctcctccac cgccggacgg
     9241 cggacggcgg cgccctttcc gcggccgcgg aggcggcggt acgggcccgg cacgaccgcc
     9301 tgctgacgga cctggatgcg gcggtgtcgt ccctgagcgc gcaccgtgcg ggcctgacgg
     9361 agcacggggt ggacatgctg cagcagtgcg cggcggttgc gcggagcgcc gtgtaaggag
     9421 cgccgcgcga ggcttgcgtg gtgcggccct ggcgggtgga cacgcgccgg acccgtagcg
     9481 tccgacgccg ccgcgcaatc cacccctacg aaccggaccg gcggtgccgt gccgtcctca
     9541 gcgtctccct gacgcccccc ggggtcaggc gcgacctcca cgtcgcccag cgctcccacc
     9601 gcgtccccca gagccgggga tcctcgtacg ggacccagcc cgtcgccccg gacgggtacc
     9661 tcggcacccc cacgcacagc cgcttcgtga tgacgaagcg cgtctcgtcc gtgctgttgg
     9721 gctgggaggc gtgcaggtgg tccccggcga agagcaggat gtccccgggg tccagctcca
     9781 cgtcgaccgg cgcaccgacc tcctgccccg gcaccagcgt gtgcccgtcg tgacgcagcg
     9841 ggcgccggta cgcgtcgggg tagaacagca gcccgttgcc gctgcggacc gggccgacgg
     9901 ccatccagag gttgacggag ttgacggcgg tgttgaacca ggaatcaaca tgggcgtcac
     9961 gcggcgtcag gaagccttcc aatccggcca gttcttcttc cccggccccg accgagcgct
    10021 ccggcggcat gatgcggatc ccgaggtgcc cgctcaggta cagccgcgct ccctgcggct
    10081 ccagggccct ggccacatcg tgcaggaccg tgcgggagaa gggccgcagc cgggtttcca
    10141 ggtcggtgcg gaccgccgcg atctgcccgg tgtccgcgtg ccggtgcgcg ttctccagcg
    10201 ccccggagac ctgcgcggcc tccgccgccg gcagggcggc ggccacggct cgggcggcct
    10261 cccgccggat cgtctccagc gcgccgatgt ggcgcagtcc gcccttgacg gccaccagcg
    10321 cgccgtcgag gacgtgccgc agcgagacgg gaatgccgta cgggccgggt tcgacgctgt
    10381 gcagggcggc gggggcgccc acggaaccgg ccgccgaacc ggcccaaccg tccacggaac
    10441 cggccgcggg cccgtcggcc ggccgggtca cggggtctcc tccccgcgct cccgggtgcc
    10501 gccggccatg gcctcgatcc cgtcgtgcag agccgtccgc gggcggaagc cgaacgcgcg
    10561 ggcggcgagg gagatgtcgg ccccggtgtg ccgggcgtcg cccggccccg gcggccgcgg
    10621 gacgacggcg acgggcccgg cgacgctctc cacggcggcg atggcctgct tcatggacac
    10681 cggcgagccg cccccgatgt tggccacccc ggtgaagccg gacagggccg cgtcgcgcat
    10741 gccggtgacg acgtcctgga cgtacgtgaa gtcacgggtc tgctcgccgt cgccgtagag
    10801 cgggaacggc gctccgcccc gcgcggcggc caggaggcgg gcgaaggcca tgtcgggccg
    10861 ctgccggggc ccgtagacgg tgaacagccg cagcgcgacg gcgggcaccc cgtaggccgt
    10921 gcggtacagc tcgcacaggt gctcggcggc gagcttggtc acgccgtagg gcgagacggg
    10981 ccgggggcac gccgtctcgg ccgtcgggaa ggcccgggcg tcgccgtaga cggaggagct
    11041 ggaggcgtag acgaacttga ccagcgggcg cccccggacg gcttccagca gcacttgggt
    11101 ggccaggatg ttccggtcgg cgtagagcgc gaactcgcgg ccccaggagg gccggatgcc
    11161 gggctgggcg gcgaggtggt agacgacgtc cgcgccgtcg agcacgggat cgagtgcggt
    11221 gtccagcagg tcggcccggt ggaaggtgaa ggccgggcag ctcatcgggc cgcgcaggtt
    11281 gcgctccttg agggcggggt cgtaggagtc ggtgagc
//
