LOCUS       NZ_BMUT01000011        10615 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces hiroshimensis strain JCM 4586 sequence11, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUT01000011
VERSION     NZ_BMUT01000011.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces hiroshimensis
  ORGANISM  Streptomyces hiroshimensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 332845)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 90X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650335.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:32:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,830
            CDSs (total)                      :: 6,745
            Genes (coding)                    :: 6,620
            CDSs (with protein)               :: 6,620
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 25 of 125
            Pseudo Genes (incomplete)         :: 107 of 125
            Pseudo Genes (internal stop)      :: 4 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:07:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 10615
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUT01000011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10615
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10615
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10615
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3146:5615](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(3147..5615)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(<1..236)
                     /locus_tag="IE241_RS35015"
                     /pseudo=""
     CDS             complement(<1..236)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007265848.1"
                     /locus_tag="IE241_RS35015"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="2-oxoglutarate ferredoxin oxidoreductase subunit
                     alpha"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAVQTVAVPHLHTSSGPPGGKTVRRMSRVVIRFAGDSGDGMQLTG
                     DRFTSETASFGNDLSTLPNFPAEIRAPAGTLPG"
     gene            448..1074
                     /locus_tag="IE241_RS23630"
                     /old_locus_tag="GCM10010324_48260"
     CDS             448..1074
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015507668.1"
                     /locus_tag="IE241_RS23630"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_48260"
                     /product="NADH oxidase"
                     /protein_id="WP_190023833.1"
                     /transl_table=11
                     /translation="MPDPVGTRPLHLWSLRGDVVVEQGEGDGDLVLRGSWGTERIANAD
                     PLVREALRRMELGPVLLANVEARREPGGDRPGSYFVLLPTLVRLSHLLVRSLGLDDFKG
                     PLLSVSPLSPDAFFAPVRLPARRRVRLPAGASLTLEPAGAGLALESSGSAHRVVLHRPE
                     AAWVAGLLAWPVTPDAASARLPLPPEVTAGILDYLAAAGMAAPVG"
     gene            complement(1106..1717)
                     /locus_tag="IE241_RS23635"
                     /old_locus_tag="GCM10010324_48270"
     CDS             complement(1106..1717)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948908.1"
                     /locus_tag="IE241_RS23635"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_48270"
                     /product="DNA-binding response regulator"
                     /protein_id="WP_190023834.1"
                     /transl_table=11
                     /translation="MVIRILLAEDMNMVRGALVALLNLEGDLEVVCELERGDEIVDAAL
                     EHRPDVAVIDIDLPGLDGLTAAARLHQRLPGCKTLILTSLGRPGTLRRALTSQVTGYLL
                     KDAPPHELASAVRRAAAGQRVIDPQLALSAWDGMDSPLTERETEVLQRAAEGLEASEIA
                     AKLHLSTGTVRNYLTVVVSKLNARNRVDAIRIAREAGWLL"
     misc_feature    complement(1496..1498)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    1703..1718
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.21"
                     /tool="antismash"
     misc_feature    1703..1721
                     /note="TFBS match to FuR_variant_2, Ferric Uptake
                     Regulator, confidence: weak, score: 22.18"
                     /tool="antismash"
     misc_feature    1756..1769
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.29"
                     /tool="antismash"
     gene            complement(1835..3022)
                     /locus_tag="IE241_RS23640"
                     /old_locus_tag="GCM10010324_48280"
     CDS             complement(1835..3022)
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037403.1"
                     /locus_tag="IE241_RS23640"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_48280"
                     /product="sensor histidine kinase"
                     /protein_id="WP_229899715.1"
                     /transl_table=11
                     /translation="MNATGQHGPSPDATAAPGDGRIESVLGRGRGLPAPRRARLILIVA
                     LLCYLSITVINLLSTSPSPVALAAGLTCLPAIFALQLWHSNPGAQRAPAHRKALTLGLQ
                     AVLTYLPLLAFHSRWGSMAGFLAGSLLLQLPARPGWLLYGLVGLSMLVPPLLDGETLLA
                     IVYLAQSTLLTGLVVYGLARLTELVRVLHDTRHELSRVAVTKERLRFARDLHDLLGFSL
                     SAITLKSELILRLIPHHPAGAVTEVGDVLAIARQSLADVRTVASGLRTMSLAQEVTSAR
                     AVLAAADVEVRGEVALDGVGARTETVLAAVLREAVTNLLRHTKATYCDISAVCTNGRVR
                     LTVVNDGVDPEHRDPSPDSGSGLGNLATRLASVNGTLTAGSSTEGIFRLCAEAPA"
     misc_feature    complement(3139..3150)
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            complement(3147..5615)
                     /locus_tag="IE241_RS23645"
                     /old_locus_tag="GCM10010324_48290"
     CDS             complement(3147..5615)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: TIGR03604"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Cyanobactin_D_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Goadsporin_CD_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NHLP_CD_RRE"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015507666.1"
                     /locus_tag="IE241_RS23645"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_48290"
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="WP_190023836.1"
                     /sec_met_domain="Cyanobactin_D_RRE (E-value: 7.6e-09,
                     bitscore: 29.8, seeds: 10, tool: rule-based-clusters)"
                     /sec_met_domain="Goadsporin_CD_RRE (E-value: 3.2e-19,
                     bitscore: 63.1, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="NHLP_CD_RRE (E-value: 1.9e-10, bitscore:
                     34.9, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="YcaO (E-value: 3.4e-89, bitscore: 294.0,
                     seeds: 127, tool: rule-based-clusters)"
                     /sec_met_domain="TIGR03604 (E-value: 3.3e-76, bitscore:
                     250.7, seeds: 25, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSGEGRTGSRPSADGGGLVGFKSHLRPTVVPGDAAYLVSPRGVTA
                     LHGSHVETLVPLLDGTRSPGAVLQDAAAVLGPEAAGASLRILLDSGLLRLRPGRPAPES
                     GPDPAAEAYWDLAGLDGGRAAAAVTGATVRIEALDGLDPGPVAAACRDSGIAVVDDGER
                     DCATTGRPGAPGGSPADFSVVLCADYLSPRLADVDAAHRAAGRPWLIAKPCGTDPWVGP
                     VFRPGDGPCWACMGARIRGHRRAEGPVRRALGLADGPLARPLASLAAGRTVAVQLAVLE
                     TAKWLAGIRHPAQGAVRTFDTLLLRAGSHPVPRLPHCAVCGDPGLVAWRVRAPFVPVSR
                     PKAARTRDLGGHRALTPEQMLSRYGHLVDPVTGIVKEIRRAPHSPDFTSCFLSGHNLAM
                     GSPTLDGLRAGLRALSGGKGLTDAEARTSALCEAVERYSGTRHGDEPVIRARYRALGAA
                     AVHPNACQLYDERQFRDRDAWNARHSRFQYVPAPFDEDRPTDWTPVWSLTAGTQRLLPT
                     STLYYSHADSRTGPPSDGLLADSNGTAAGSSPEDALLQGFLELVERDAVALWWYNRTRH
                     PAVNLPAFADPGLDRLREGYLRLGRELWVLDLTTDLGIPVMAALSRRTDRATHGTQAAH
                     GRRAMPAPPAAQDIVFGFGAHFDPRLALRRALTEMGQLLPAVHGKTERKTAGTGGADAA
                     GGGAAYATDDPEACAWWQHATTANQPYLLPDPHVPARGPGDWPYTPRADLLDDVTAVID
                     LTRRHGMELLVLDQTRPDTGIPVIRVIVPGLRHFWARFAPGRLFDVPVSLGRTSRPTAY
                     EDLNPVPLFV"
     aSDomain        complement(5259..5561)
                     /aSDomain="Goadsporin_CD_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing CD fusion protein in a
                     goadsporin cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IE241_RS23645_Goadsporin_CD_RRE.1"
                     /evalue="3.00E-20"
                     /identifier="RREFam013.1"
                     /label="IE241_RS23645"
                     /locus_tag="IE241_RS23645"
                     /protein_end="119"
                     /protein_start="18"
                     /score="63.1"
                     /tool="antismash"
                     /translation="VGFKSHLRPTVVPGDAAYLVSPRGVTALHGSHVETLVPLLDGTRS
                     PGAVLQDAAAVLGPEAAGASLRILLDSGLLRLRPGRPAPESGPDPAAEAYWDLAGL"
     aSDomain        complement(5331..5561)
                     /aSDomain="NHLP_CD_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing CD fusion protein in a
                     NHLP/Nif11 LAP cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IE241_RS23645_NHLP_CD_RRE.1"
                     /evalue="1.80E-11"
                     /identifier="RREFam015.1"
                     /label="IE241_RS23645"
                     /locus_tag="IE241_RS23645"
                     /protein_end="95"
                     /protein_start="18"
                     /score="34.9"
                     /tool="antismash"
                     /translation="VGFKSHLRPTVVPGDAAYLVSPRGVTALHGSHVETLVPLLDGTRS
                     PGAVLQDAAAVLGPEAAGASLRILLDSGLLRL"
     aSDomain        complement(5349..5564)
                     /aSDomain="Cyanobactin_D_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing D protein in a cyanobactin
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IE241_RS23645_Cyanobactin_D_RRE.1"
                     /evalue="7.20E-10"
                     /identifier="RREFam007.1"
                     /label="IE241_RS23645"
                     /locus_tag="IE241_RS23645"
                     /protein_end="89"
                     /protein_start="17"
                     /score="29.8"
                     /tool="antismash"
                     /translation="LVGFKSHLRPTVVPGDAAYLVSPRGVTALHGSHVETLVPLLDGTR
                     SPGAVLQDAAAVLGPEAAGASLRILLD"
     gene            complement(5612..5821)
                     /locus_tag="IE241_RS23650"
                     /old_locus_tag="GCM10010324_48300"
     CDS             complement(5612..5821)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE241_RS23650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010324_48300"
                     /product="hypothetical protein"
                     /protein_id="WP_190023837.1"
                     /transl_table=11
                     /translation="MDICDPFGSPATGPAAARSAFPRPFLVIENLDAPDGDGLALFTQI
                     CIIAPPVMSPAAGAGTGTAEPAAA"
     misc_feature    5819..5834
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 15.81"
                     /tool="antismash"
     misc_feature    5821..5835
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.57"
                     /tool="antismash"
     misc_feature    5927..5939
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.11"
                     /tool="antismash"
     gene            6000..6311
                     /locus_tag="IE241_RS23655"
                     /old_locus_tag="GCM10010324_48310"
     CDS             6000..6311
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039972.4"
                     /locus_tag="IE241_RS23655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_48310"
                     /product="DUF6059 family protein"
                     /protein_id="WP_190023838.1"
                     /transl_table=11
                     /translation="MSGSDARGPLSALRSALAPALRSALRACLLSAMNALRTQGQFWVY
                     DPSPGPSPAPSPATRPLRGPAPGHPERLCPEAVPTAGELLLYRQVFGDGSGGTGPGGP"
     gene            complement(6373..6825)
                     /locus_tag="IE241_RS23660"
                     /old_locus_tag="GCM10010324_48320"
     CDS             complement(6373..6825)
                     /GO_function="GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018957860.1"
                     /locus_tag="IE241_RS23660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_48320"
                     /product="CoA-binding protein"
                     /protein_id="WP_190023839.1"
                     /transl_table=11
                     /translation="MYAEPGIIRKIIEETGDTWALVGLSSNTRRAAYGVAEVLRRHGKR
                     VVPVHPKAEEVHGEQGYASLADIPFAVDVVDVFVNSDLAGPVADEAVAIGAKAVWFQLG
                     VVDEEAYERTRAAGLDMVMDRCPAIELASLRRRDARGVRPVREPLA"
     misc_feature    6888..6901
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.52"
                     /tool="antismash"
     gene            6904..7530
                     /locus_tag="IE241_RS23665"
                     /old_locus_tag="GCM10010324_48330"
     CDS             6904..7530
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948434.1"
                     /locus_tag="IE241_RS23665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_48330"
                     /product="YigZ family protein"
                     /protein_id="WP_190023840.1"
                     /transl_table=11
                     /translation="MTEQYRTVAREGVHEIEISKSRFICALAPAATEEEAQAFIARIRK
                     EHPTARHHCFAYVLGADGSVQKASDDGEPGGTAGVPMLQMLLRREVRYAVAVVTRYFGG
                     VKLGAGGLIRAYGGVVGEALDVLGTVTRQRFRLAAVTVDHQRAGKLENDLRATGRAVRE
                     VSYGAEVRIEVGLPEADVEGFRAWLADATAGTARLELGGEAYGDA"
     gene            complement(7574..8545)
                     /locus_tag="IE241_RS23670"
                     /old_locus_tag="GCM10010324_48340"
     CDS             complement(7574..8545)
                     /EC_number="3.-.-.-"
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1235: polysaccharide deacetylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF013672.5"
                     /locus_tag="IE241_RS23670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_48340"
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="WP_190023841.1"
                     /transl_table=11
                     /translation="MRLIRNDFGRRGGARSSIRRRPVRPLALVLTAVLLLAVGVACATR
                     DGSLPGTGDGQNMAGLGQRPGQAHRQAPPPQQPPPVPKVKQSPEQRLAAAQRWGLDEAP
                     LVAPPPPKGKPELSTPEWADKGSGLIPAPASVPTTEKIAFLTIDDGAAKDREFLDMVRE
                     LQVPLSAFLTDESAAEDYGYFRDLHALGVSVQNHTLRHPQLNHVSEDEKYNEICGQQEI
                     LEREIGVRPTLFRPPFGDFDAETLAVAEDCGVRAAPLWNEEAFADRIDFRHDDQQFHPG
                     DIILTHFNGPRAWGGTMCDMLRTVLRKLTEQGYALARLEDYA"
     gene            8645..9814
                     /locus_tag="IE241_RS23675"
                     /old_locus_tag="GCM10010324_48350"
     CDS             8645..9814
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Metallophos_2"
                     /gene_functions="other (smcogs) SMCOG1230: Nuclease SbcCD,
                     D subunit"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019884525.1"
                     /locus_tag="IE241_RS23675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_48350"
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="WP_190023842.1"
                     /sec_met_domain="Metallophos_2 (E-value: 2.5e-08, bitscore:
                     28.4, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRILHTSDWHLGRSFHRVNLLDAQRAFIGHLVRTVRDERIDVVLV
                     AGDVYDRAVPPLGAVALFDDALHRLADLGVPTVMISGNHDSSRRLGVGAGLLDRAGIHL
                     RTDPAGCATPVVLTDAHGDVAFYGLPYLEPALVREQLGAERAGHTAVLEAAMDRVRADL
                     AARPAGTRSVVLAHAFVTGGTTSDSERDITVGGVAQVPAAVFDGVDYAALGHLHGCQTI
                     TERVRYSGSPLAYSFSEAGHRKSMWLIDLDAGGAVTAERLDCPVPRPLARIRGRLDDLL
                     QDPRLARHEDAWVEATLTDPHRPDEPMARLAARFPHVLSLVLDPERPPRDPLASYAHRL
                     RDRSDREIAEDFVAHVRDGLGPDDAERKILTDALDHVRLAAAGEEASAR"
ORIGIN
        1 acacccggca gggtcccggc aggggcgcgg atctcggcgg ggaagttcgg cagagtggag
       61 aggtcgttgc cgaacgaggc ggtctcggac gtgaaacggt cacccgtcag ctgcataccg
      121 tccccggaat cacccgcgaa acggatgacg acccggctca ttcgccgtac cgtctttccc
      181 cccggaggcc cgctgctcgt gtgcaggtgc gggaccgcca ctgtctgaac ggccatagag
      241 gccgccccct tcccgtcgtc gccggtgagt gtgagcgtgc tgccgtcgca cggtaggaag
      301 ggcgacttgc gacaggccaa agcgggacgt gaaatcttca cggtccttct gtgaacccgt
      361 ggatgttgct ccacggccct cccgtggata ctcggtcgcg tccgtccaga gcctcctgga
      421 gagcgcgcgg agagagaggg accagcgatg ccggatccgg tcggaacccg accgttgcac
      481 ctctggtcgc tgcgcggcga cgtggtcgtc gagcagggtg agggggacgg tgatctcgtc
      541 ctgcgcggga gctgggggac cgagcggatc gccaatgccg acccgctcgt gcgcgaggcc
      601 ctgcgccgga tggagctcgg gcccgtcctc ctggccaacg tggaggcgcg gcgcgagccc
      661 gggggcgacc gccccggttc gtacttcgtg ctgctgccca ccctcgtgcg gctctcgcac
      721 ctcctggtgc gctcgctggg cctcgacgac ttcaagggcc cgctgctgtc ggtgtcgccg
      781 ctgtcgccgg acgccttctt cgcccccgta cggctgcccg cgcggcgccg ggtgcggctg
      841 cccgcggggg catccctcac cctggagccc gcgggcgcgg gcctggccct ggagtcgtcc
      901 ggcagtgcgc accgcgtggt gctgcaccgg cccgaagcgg cgtgggtggc ggggctgctg
      961 gcctggccgg tcacgccgga cgccgcctcc gcgaggctgc ccctgccgcc cgaggtgacg
     1021 gcgggcatcc tcgactacct ggccgccgcc ggcatggccg cccccgtggg gtagctccgg
     1081 gccggccgcc agggatgcgg ggccgctaca ggagccagcc cgcctcccgc gcgatgcgga
     1141 tcgcgtccac ccggttgcgt gcgttgagct tggagaccac gaccgtcagg tagttgcgga
     1201 cggtgcccgt ggacaggtgc agcttcgcgg cgatctccga cgcctccagc ccctccgcgg
     1261 cccgctgcag cacttccgtc tcgcgctcgg tcagcggcga gtccatgccg tcccaggcgc
     1321 tcagggcgag ctgggggtcg atgacgcgct gtcccgcggc cgcgcgccgt accgccgagg
     1381 cgagttcgtg gggcggagcg tctttcaaga gatagccggt gacctgggag gtcagcgccc
     1441 ggcgcagggt gcccgggcgg cccaggctgg tgaggatcag ggtcttgcac ccgggtaacc
     1501 gctggtgcag ccgggccgcc gccgtgaggc cgtcgagacc cgggaggtcg atgtcgataa
     1561 ccgcgacatc cggccggtgt tccagggcgg cgtcgacgat ttcgtcaccg cgctcgagtt
     1621 cgcagacgac ttcgaggtcc ccttcgagat tcagcaatgc gacgagagct ccccggacca
     1681 tgttcatgtc ctcggcgagc agaatgcgaa tcaccatgaa tgagcccccc gttccctcat
     1741 ggtgtgcacc gacgatggaa tcaacataac ggtttgtcaa ggactttgac ctgtgcgccg
     1801 ggccgtgcgg gcactctcct tctccgtcgt accctcaggc cggggcttcc gcacacaacc
     1861 ggaatattcc ctccgtggag ctgccggcgg tgagggttcc gttcaccgag gccagccgcg
     1921 tcgccaggtt ccccaggccg ctgccgctgt ccggggacgg gtcgcggtgc tcggggtcca
     1981 ccccgtcgtt gacgacggtg agccggaccc ggccgttcgt gcagacggca ctgatgtcgc
     2041 agtatgtggc cttggtgtgc cggagcaggt tggtgacggc ctcgcggagc acggccgcga
     2101 ggacggtctc ggtccgcgcg ccgaccccgt cgagcgcgac ctcgccccgt acctcgacgt
     2161 ccgccgcggc cagcacggcc cgcgcggagg tcacctcctg cgccagggac atggtgcgca
     2221 gcccgctcgc cacggtgcgg acgtcggcga gcgactgccg ggcgatcgcc agcacgtcgc
     2281 cgacctccgt cacggcgccc gcggggtggt gcgggatcag ccgcaggatc agctcgctct
     2341 tgagggtgat cgcggagagg ctgaagccga gaaggtcatg caggtcccgc gcgaaccgca
     2401 ggcgctcctt ggtcaccgcc acccgcgaca gctcgtgccg ggtgtcgtgc agcacccgta
     2461 cgagctccgt gagccgcgcc aggccgtaga cgacgaggcc ggtcaggagc gtcgactggg
     2521 cgaggtagac gatcgcgagg agggtttcgc cgtccaggag cggcgggacc agcatggaga
     2581 gcccgacgag gccgtacagc agccagccgg gccgtgcggg gagctggagg agcagcgaac
     2641 cggccaggaa gccggccatg gagccccagc gggagtggaa ggccagcagt ggcagatagg
     2701 tgaggacggc ctggaggccg agcgtgaggg ccttgcggtg cgcgggggcc cgctgcgccc
     2761 ccggattcga gtgccacaac tgcagggcga agatggccgg aaggcaggtc agcccggccg
     2821 caagggcgac cggcgacggg ctggtgctga gcaggttgat gacggtgatg ctcagatagc
     2881 acagcagggc cacgatcagg atgagccggg cccgccgcgg agcgggcagg ccacggccgc
     2941 gcccgagcac gctctcgatc cgcccgtcgc cgggcgccgc ggtggcgtcc ggcgacgggc
     3001 cgtgctgtcc ggtggcgttc aagaccccta cttccacact tccgcatgag agtgcgacgg
     3061 ctgccggaag cataagtgag gggcggcgta cgggatatga cgaggagggc gcatctcgcg
     3121 ccctccgcgg ggccttttcc gttcgtttac acgaacaggg ggacgggatt gaggtcttcg
     3181 tacgcggtgg ggcgggacgt cctgcccagg gataccggaa cgtcgaagag acggcccggg
     3241 gcgaatcggg cccagaagtg ccggaggccc ggaacgatca cacggatgac cggaattccg
     3301 gtgtccggtc tggtctggtc gagaaccagg agttccattc cgtgccggcg ggtcaggtcg
     3361 atcaccgccg tcacgtcgtc cagcaggtcc gcgcgcggcg tgtacggcca gtcgccgggg
     3421 ccgcgggcgg ggacgtgcgg atcgggcagc aggtagggct ggttggcggt ggtggcgtgc
     3481 tgccaccacg cgcacgcctc cgggtcgtcg gtcgcgtacg cagcccctcc gccggctgcg
     3541 tccgcaccgc ccgtccctgc cgtcttcctt tccgtcttcc cgtgcaccgc gggcagcagc
     3601 tgccccatct cggtgagagc ccggcgcagc gccaggcggg ggtcgaagtg cgccccgaag
     3661 ccgaagacga tgtcctgcgc cgccggtggg gccggcatcg ctcgtcggcc gtgcgccgct
     3721 tgcgtgccgt gcgtcgcccg gtcggtgcgg cgggacagcg ccgccatgac gggaatgccg
     3781 agatccgtgg tgagatcgag cacccacagc tcgcgcccca gtcgcagata cccctcccgc
     3841 agccggtcga gtcccgggtc ggcgaacgcg ggcaggttga ccgccgggtg gcgggtgcgg
     3901 ttgtaccacc acagggcgac cgcgtcccgc tcgacgagct ccaggaagcc ctgcagcagc
     3961 gcgtcctcgg ggctgctgcc ggcggcggtg ccgttggagt cggccagcag gccgtcggac
     4021 ggcggcccgg tacggctgtc ggcgtggctg tagtagagcg tcgaggtcgg caggagccgc
     4081 tgggtcccgg cggtcagcga ccatacgggc gtccagtcgg tgggccggtc ctcgtcgaag
     4141 ggtgcgggta cgtactggaa gcgggagtgg cgggcgttcc aggcgtcccg gtcccggaac
     4201 tgccgctcgt cgtagagctg gcaggcgttg gggtggacgg cggcggcgcc gagggcgcgg
     4261 tagcgggcgc ggatcaccgg ttcgtcgccg tgccgggtgc cgctgtagcg ctcgacggcc
     4321 tcgcacaggg cgctggtgcg ggcctcggcg tcggtgagcc ccttgccccc gctgagcgcc
     4381 cgcagcccgg cccgcaggcc gtcgagggtg ggggagccca tggccaggtt gtggccggag
     4441 aggaagcagc tcgtgaagtc ggggctgtgc ggggcccggc ggatctcctt gacgatgccg
     4501 gtgaccgggt cgacgagatg cccgtaccgg ctcagcatct gctcgggcgt cagggcgcgg
     4561 tggccgccca ggtcgcgggt gcgggcggcc ttgggccgcg agaccgggac gaacggcgcc
     4621 cgcacgcgcc acgcgacgag ccccgggtcg ccgcagaccg cgcagtgcgg cagccggggg
     4681 accgggtggc tgcccgcgcg cagcagcagg gtgtcgaagg tgcggacggc gccctgtgcg
     4741 ggatgccgga tgccggccag ccacttggcc gtctccagca ccgcgagctg cacggccacc
     4801 gtccggcccg cggccaggga ggccaggggc cgggccagcg gaccgtccgc caggccgagc
     4861 gcccgccgta cgggaccctc ggcccggcgg tgcccgcgta tccgggcccc catgcaggcc
     4921 cagcagggcc cgtcgccggg gcggaagacg gggccgaccc aggggtcggt gccacagggc
     4981 ttggcgatca gccagggccg gccggccgcc cggtgcgcgg cgtccacgtc cgcgagccgg
     5041 ggcgagaggt agtcggcaca caggacgacg gagaagtcgg ccggggatcc gccgggagcc
     5101 ccgggcctcc ccgtcgttgc gcagtcgcgc tcgccgtcgt ccacgacggc gattcccgaa
     5161 tcccggcacg ccgcggcgac aggccccgga tcgaggccgt ccagcgcctc gatccggacg
     5221 gtggcgccgg tgacggccgc ggccgcccgg cccccgtcga gtccggcgag gtcccagtag
     5281 gcctcggccg ccgggtcggg gccggactcc ggggccggcc ggcccgggcg cagccggagc
     5341 agcccggagt cgagcaggat ccgcagcgag gcgccggccg cctcgggacc gagcaccgcg
     5401 gccgcgtcct gcaggacggc gcccgggctg cgggtcccgt ccagcagggg gacgagggtt
     5461 tcgacgtggg agccgtgcag cgccgtgacg ccgcgcggcg agacgaggta ggcggcatcg
     5521 cccggcacga cggtgggacg caggtggctc ttgaacccca ccagcccccc gccgtcggcg
     5581 gacggccggg atccggttct cccctcgccg ctcatgccgc ggccggctcc gcggtaccgg
     5641 taccggcacc ggccgccggg gacatcaccg ggggagcgat gatgcagatc tgggtgaaga
     5701 gggccaggcc gtccccgtcc ggtgcgtcga ggttctcgat cacgaggaac ggccggggga
     5761 acgcggagcg ggcggcggca gggccggtgg ccggtgaacc gaacgggtcg cagatgtcca
     5821 tggagatcgt tctccttcat cgtgctgccg gatttcttcg gtgggcgtgg tgacgattct
     5881 gcgaaggagg actggagcct gacagtgcgg tcgtcatctg tgccgggtga agttttcacg
     5941 gctgccgcag gcggcccggg ctttggcgga actttggcca tcggatgcac gctcgcccga
     6001 tgtccggttc cgacgcccgc ggtcctctgt ctgcgctccg gtcggctctc gccccggccc
     6061 tgcgctcggc cctgcgcgcc tgcctgcttt cggccatgaa cgcgctgcgc acccagggcc
     6121 agttctgggt ctacgacccc tctcccggcc cctctcccgc cccgtccccc gccacccggc
     6181 ccctgcgcgg cccggccccc gggcaccccg agcggctgtg cccggaagcc gtgccgacgg
     6241 ccggggagct cctgctctac cggcaggtgt tcggtgacgg aagcggcggc accggccccg
     6301 gcggaccgtg aacggatgac gcacgccgcc gtaccgccgt accaccgccg tcaccggcgg
     6361 aagcacccgc cgtcaggcga gcggctccct caccgggcgc accccgcggg cgtcgcgccg
     6421 acgtagcgac gcgagttcga ttgcggggca ccggtccatc accatgtcca gaccggccgc
     6481 gcgcgtgcgc tcgtaggcct cctcgtccac gacccccagc tggaaccaga ccgccttcgc
     6541 gccgatcgcg acggcctcgt ccgcgaccgg gcccgcgagg tcgctgttga cgaacacgtc
     6601 caccacgtcg accgcgaagg ggatgtccgc gagcgaggcg tagccctgct cgccgtggac
     6661 ctcctcggcc ttggggtgca ccggcaccac gcgcttgccg tggcgccgca gcacctcggc
     6721 cacgccgtag gccgcccgcc gcgtgttgga cgacagaccg accagcgccc aggtgtcgcc
     6781 ggtctcctcg atgatcttcc ggatgatccc cggttctgcg tacatgcccg ctcaacgatc
     6841 atccccccgt caccattccc gggcccccgt cacccttccc gggccggcgc ataggcttgc
     6901 gggatgacgg agcagtaccg gacggtcgcg cgcgagggcg tgcacgagat cgagatcagc
     6961 aagtcgcgct tcatctgcgc gctcgcgccc gccgccaccg aggaggaggc gcaggcgttc
     7021 atcgcgcgca tccgcaagga gcaccccacc gcccgccacc actgtttcgc ctacgtgctg
     7081 ggcgccgacg gctcggtgca gaaggcgagc gacgacggcg agccgggcgg caccgccggc
     7141 gtgcccatgc tgcagatgct gctccgccgc gaggtccgct acgccgtggc cgtcgtcacc
     7201 cgctacttcg gcggagtcaa gctcggcgcg ggcgggctga tccgcgccta cgggggcgtg
     7261 gtcggcgagg ccctggacgt gctcgggacc gtcacccggc agcgcttccg gctggcggcc
     7321 gtgaccgtcg accaccagcg cgccggaaag ctggagaacg acctgcgggc gacggggcgg
     7381 gccgtgcgcg aggtgagcta cggcgcggag gtccgcatcg aggtggggct gcccgaggcg
     7441 gacgtcgagg gcttccgcgc ctggctcgcc gacgccacgg ccggcacggc ccgcctcgaa
     7501 ctgggcggcg aggcctacgg ggacgcctga gcccggcccg gcctgaaccg ggccgtttcc
     7561 gcaggccgcc gcgtcaggcg tagtcctcca gccgcgccag cgcgtagccc tgctccgtga
     7621 gcttgcgcag caccgtccgc agcatgtcgc acatggtgcc gccccacgcc ctgggcccgt
     7681 tgaagtgggt gaggatgatg tcgccggggt ggaactgctg gtcgtcgtgg cggaagtcga
     7741 tccggtccgc gaaggcctcc tcgttccaca ggggcgcggc ccggaccccg cagtcctcgg
     7801 cgacggcgag ggtctcggcg tcgaagtcgc cgaacggcgg gcggaagagc gtcggccgga
     7861 cgccgatctc ccgctccagg atctcctgct ggccgcagat ctcgttgtac ttctcgtcct
     7921 cggatacgtg gttcagctgc gggtggcgca gcgtgtggtt ctggacggag acgccgaggg
     7981 cgtgcaggtc gcggaagtag ccgtagtcct cggccgcgga ctcgtcggtg aggaaggcgg
     8041 acagcggcac ctgcagctcc cgcaccatgt ccaggaactc ccggtccttc gccgcaccgt
     8101 cgtcgatcgt caggaaggcg atcttctcgg tggtcggcac ggaggcgggc gcggggatga
     8161 gccccgatcc cttgtcggcc cactccggcg tggacagctc cggtttcccc ttcggcgggg
     8221 gcggtgcgac gaggggtgcc tcgtccaggc cccagcgctg tgccgcggcg agccgctgct
     8281 cggggctctg cttcaccttc ggcaccggtg gcggctgctg cggtggcggc gcctgccggt
     8341 gggcctgtcc cggtctctgc ccgaggccgg ccatgttctg gccgtccccg gtgccgggca
     8401 gggaaccgtc gcgggtcgcg caggccactc cgaccgcgag cagcaggacc gcggtcagca
     8461 cgagggcgag ggggcggacg gggcgtcgtc gtatggagct ccgggcaccg ccgcgccgac
     8521 cgaagtcatt tcgtataagt cgcatatttc cggatccttt cagcgggtcg ccgcccccgc
     8581 ccgcccggtt cacccatcgg ggtcgtgccc cggcacctgc ccgcagatgg aacacttggg
     8641 gcccgtgagg atcctgcata cgtccgactg gcacctgggc cgctcgtttc accgggtgaa
     8701 cctgctggac gcccagcgcg ccttcatcgg ccacctcgtc cgcaccgtcc gggacgagcg
     8761 gatcgacgtg gtgctcgtcg cgggcgacgt ctacgaccgg gcggtccccc cgctcggcgc
     8821 cgtcgccctg ttcgacgacg cgctgcaccg gctcgcggac ctcggcgtgc ccaccgtcat
     8881 gatctccggc aaccacgact cctcccgccg cctgggcgtg ggcgcgggcc tcctcgaccg
     8941 ggccggcatc cacctgcgca ccgacccggc cggctgcgcc acccccgtcg tcctcaccga
     9001 cgcccacggc gacgtcgcct tctacggcct gccctacctc gagcccgccc tcgtccgcga
     9061 gcagctgggc gcggagcgcg ccggccacac ggccgtgctg gaggccgcca tggaccgcgt
     9121 ccgcgccgac ctcgccgcgc ggcccgccgg cacccgctcc gtcgtcctcg cgcacgcctt
     9181 cgtcaccggc ggcaccacca gcgacagcga gcgcgacatc accgtcggcg gcgtcgccca
     9241 ggttcccgcc gcggtcttcg acggcgtcga ctacgccgcc ctaggccacc tgcacggctg
     9301 ccagaccatc accgagcgcg tccgctactc cggctccccg ctggcctact ccttctccga
     9361 ggccggccac cgcaagtcca tgtggctgat cgacctggac gccggcggcg ccgtgaccgc
     9421 cgagcggctc gactgcccgg tgccgcgccc cctcgcccgc atccgcggcc gcctcgacga
     9481 cctgctgcag gacccgcggc tcgcccgcca cgaggacgcc tgggtcgagg ccaccctcac
     9541 cgacccgcac cgcccggacg agcccatggc ccgcctcgcc gcccgcttcc cccacgtgct
     9601 cagcctcgtc ctcgaccccg agcgcccgcc ccgggacccc ctcgcctcct acgcgcaccg
     9661 gctgcgcgac cgcagcgacc gcgagatcgc cgaggacttc gtggcccacg tgcgcgacgg
     9721 cctgggaccc gacgacgccg aacggaagat cctcaccgac gccctcgacc acgtacggct
     9781 ggccgcggcc ggcgaggagg cgagcgcccg atgaggctgc accggctgac cgtcaccgcc
     9841 ttcggcccct tcggcggcac ccagcacatc gacttcgacg cgctctccgc cgacggcctg
     9901 ttcctcttcc acggccccac cggagcgggc aagacctccg tcctcgacgc cgtctgctac
     9961 gccctctaca acgaggtgcc cggcacccgc cagggcagcg gcgcccccgt gctgcgcagc
    10021 gaccacgccg ccgacggcac cctcacccag gtggtgctcg acctcaccgt cggcgggcgg
    10081 cggctggaga tcacccggcg ccccgagcag ctgcgccgca agaagaaggg caccggcttc
    10141 accaaggaga gggccgctag cgagctgcgc gagcaccgcg ccggggcgtg ggaggcgctc
    10201 agccgctccc accaggagat cggcgaggag atcaagcagc tgctcggcat gagccgcgag
    10261 cagttctgcc aggtcgtgct gctgccccag ggcgagttcg cccgcttcct cggcgccaag
    10321 gacgccgccc gcggcgaact cctcggcagg ctcttcgaca ccggccgctt cggcgccgtc
    10381 gaaaagcgcc tcgcggagct gcgccgggcc gcggaggccg gcgtgcgggc cggcgacgac
    10441 gggctgctcg ccctcgccca ccggatgcgc caggccgccg gagacccgtc gctgcccgag
    10501 gtgggcacgc cctcgcccgc agcagaggcc gagggcggca ccggcgcacc cgcccaccgc
    10561 acccgcgacg gcgtcccggc accccgcacc gcgacgccgg cccgcagggg ccggg
//
