LOCUS       NZ_BMUT01000010        10399 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces hiroshimensis strain JCM 4586 sequence10, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUT01000010
VERSION     NZ_BMUT01000010.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces hiroshimensis
  ORGANISM  Streptomyces hiroshimensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 360801)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 90X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650335.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:32:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,830
            CDSs (total)                      :: 6,745
            Genes (coding)                    :: 6,620
            CDSs (with protein)               :: 6,620
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 25 of 125
            Pseudo Genes (incomplete)         :: 107 of 125
            Pseudo Genes (internal stop)      :: 4 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:07:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 67266
            Orig. end                         :: 77665
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUT01000010.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5399
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            184..1152
                     /locus_tag="IE241_RS22335"
                     /old_locus_tag="GCM10010324_45610"
     CDS             184..1152
                     /GO_function="GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175076.1"
                     /locus_tag="IE241_RS22335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_45610"
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="WP_190023585.1"
                     /transl_table=11
                     /translation="MATSRIAPAAPTTPAAADRTARLAVTVFPLLVVAAGALGLAAPSH
                     FTGWAPAVPYLLGVVMFTMGLTLTGDDFRAVAKRPWAVGLGLVAHYVIMPGLGWLIATV
                     LGLPPQLAAGVILVGCAPSGTASNVVTYLARGDVALSVSVATVSTLVAPLVTPPLTLLL
                     ADRFLDVDAGAMFTDILKTVLVPVVGGALVRMAAGRWIGRVLRALPALSALTIAVLVLI
                     VVSGSAARIKDAAALVLLAVVLHNGLGLALGYAAGRLARLGGPSSRAMAFEVGMQNSGL
                     AASLATAHFSPAAALPAAVFSVWHNVSGAAVAAWMSRRPHS"
     gene            complement(1495..2595)
                     /locus_tag="IE241_RS22340"
                     /old_locus_tag="GCM10010324_45620"
     CDS             complement(1495..2595)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003960017.1"
                     /locus_tag="IE241_RS22340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_45620"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_190023586.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 4.5e-18, bitscore:
                     59.9, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDSGVLHIKGRVLVGADPDGGVRDELWAVDGRITFERPAGARDV
                     TTVEGWALPGLVDAHCHVGLDAHGPVDASTAEKQALTDRDAGALLLRDAGSPSDTRWID
                     DREDLPRIIRAGRHIARTRRYIRNYAHEIEPDDLVAYVAREARRGDGWVKLVGDWIDRE
                     TGDLSACWPRGEVAAAIAEAHRLGARVTAHCFAEESLAPLVEAGIDCIEHATGLTEETI
                     PLFAERGVAIVPTLVNIATFPRLADGGEAKFPRWADHMRRLHARRYETVRAAYDAGIPV
                     FVGTDAGGSLAHGLVGQEVAELVKAGLPPLAALSAATWSARAWLGRPGLEEGAPADLVV
                     YETDPRADVRVLGSPKRVILRGRVIG"
     misc_feature    2719..2733
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 17.28"
                     /tool="antismash"
     misc_feature    2837..2852
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            3009..3596
                     /gene="ectA"
                     /locus_tag="IE241_RS22345"
                     /old_locus_tag="GCM10010324_45630"
     CDS             3009..3596
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987798.1"
                     /locus_tag="IE241_RS22345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_45630"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_190023587.1"
                     /transl_table=11
                     /translation="MTAAQADLARARPASLREFPEMPEDLTLDVPRVEDGAAIWRIARD
                     SGTLDLNSSYSYLLWCRDFAATSAVARPAGGGTGGKGGEPAGFITGYVRPARPRTLVVW
                     QVAVDRPHRGRGLAAALLEGLVARVARESEGGIDGIETTVTPGNTASNRLFASFAERHR
                     APVEREVLFHGGLFPETGHDPEVLHRIGPLTH"
     gene            3674..4942
                     /gene="ectB"
                     /locus_tag="IE241_RS22350"
                     /old_locus_tag="GCM10010324_45640"
     CDS             3674..4942
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-365). E-value: 4.7e-73.
                     Score: 238.1. Matches aSDomain:
                     nrpspksdomains_IE241_RS22350_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="IE241_RS22350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_45640"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_190023588.1"
                     /sec_met_domain="Aminotran_3 (E-value: 3.7e-77, bitscore:
                     254.0, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRSWPAVFDRAQGSRMYDEDGHTY
                     LDFFAGAGALNYGHNNPVLKRALIDYIERDGVTHGLDMGTTAKRAFLEAFQDVVLRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDHYLDDRVPDFLWFERLLADQGSGLNTPAAVIVETVQGEGGINVARP
                     EWLRGLAALCERHDMLLIVDDIQMGCGRTGSFFSFEEAGITPDIVTLSKSIGGYGLPMS
                     LTLFRPELDLWEPGEHNGTFRGNNPSFVTAAAALDAYWRDGQMEKQTLARGEQAEEALR
                     AICTEHPELGGHYRGRGLVWGLEFADKARASAVAGRAFELGLLVETSGPEGEVVKLLPA
                     LTISPEELDEGLRILARAVRETA"
     aSDomain        3758..4768
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE241_RS22350_Aminotran_3.1"
                     /evalue="4.70E-73"
                     /label="IE241_RS22350_Aminotran_3.1"
                     /locus_tag="IE241_RS22350"
                     /protein_end="365"
                     /protein_start="28"
                     /score="238.1"
                     /tool="antismash"
                     /translation="FDRAQGSRMYDEDGHTYLDFFAGAGALNYGHNNPVLKRALIDYIE
                     RDGVTHGLDMGTTAKRAFLEAFQDVVLRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDHYLDDRVPDFLWFER
                     LLADQGSGLNTPAAVIVETVQGEGGINVARPEWLRGLAALCERHDMLLIVDDIQMGCGR
                     TGSFFSFEEAGITPDIVTLSKSIGGYGLPMSLTLFRPELDLWEPGEHNGTFRGNNPSFV
                     TAAAALDAYWRDGQMEKQTLARGEQAEEALRAICTEHPELGGHYRGRGLVWGLEFA"
     gene            5001..5399
                     /locus_tag="IE241_RS22355"
                     /old_locus_tag="GCM10010324_45650"
     CDS             5001..5399
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="IE241_RS22355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_45650"
                     /product="ectoine synthase"
                     /protein_id="WP_058044967.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.7e-55, bitscore:
                     179.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLQDIENTDRHVKAKSGTWESKRIVLAKERVGFSLHETVLY
                     AGTETSMWYANHIEAVLCVEGEAELTDDETGEKHWISPGTMYLLDGHERHTLRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEG"
     gene            5403..6320
                     /gene="thpD"
                     /locus_tag="IE241_RS22360"
                     /old_locus_tag="GCM10010324_45660"
     CDS             5403..6320
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130465.1"
                     /locus_tag="IE241_RS22360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_45660"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_190023589.1"
                     /sec_met_domain="PhyH (E-value: 1.3e-36, bitscore: 121.2,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAAVTDLYPTRGTHEAAVPRRDPVVWPGSGSASASGSGSRTPGPI
                     DAAALAGYERDGFLTVDEMITPGEVQVLRAELDRLIADPAVRADERAVVEPHSQDVRSV
                     FEVHRISDVFARLVRDPRVVGRARQILGSDVYVHQSRVNVKPGFGASGFYWHSDFETWH
                     AEDGLPRMRTVSVSIALTENLDTNGGLMIMPGSHRTFLGCAGVTPQDNYKRSLRMQEAG
                     TPSDSALAALAGRHGIRLFTGPAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENT
                     AVDPFAAPSRRPEFVGARDFTPVG"
     gene            complement(6417..7457)
                     /locus_tag="IE241_RS22365"
                     /old_locus_tag="GCM10010324_45670"
     CDS             complement(6417..7457)
                     /NRPS_PKS="Domain: Aminotran_5 (13-341). E-value: 1.1e-27.
                     Score: 88.9. Matches aSDomain:
                     nrpspksdomains_IE241_RS22365_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180697.1"
                     /locus_tag="IE241_RS22365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_45670"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_190023590.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.2e-22, bitscore:
                     73.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MESLAPAHFAPETVYLNTAATGLVPAPAVAAMTEAATAFSLGRPG
                     AEDHFPVVEAVRAAFARLVGVPARQVATGGSAAVFCGLVAQSLPEGSEVLCAEGDFSSL
                     VNPFHVRPGLGVRTVPLEEVAGAVRPGTALVAVSAVQSADGRIADLGAITAAARAHGAR
                     TLVDISQAAGWLPVDAGAYDFTVCVGYKWLMGPRGTAFLTVPEDLGGLTPVFAGWVAGE
                     NPWDSTYGKVSLARSARRYDENPALLPYAGALGGLRLIEELGTERIAAHDRALAERFRA
                     GLAALGHRPVAAPDSAVVSVPGLGHAAERLAAEGVRVSARAGGLRASFHLYNSATDVDR
                     VLKTLS"
     aSDomain        complement(6435..7418)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE241_RS22365_Aminotran_5.1"
                     /evalue="1.10E-27"
                     /label="IE241_RS22365_Aminotran_5.1"
                     /locus_tag="IE241_RS22365"
                     /protein_end="341"
                     /protein_start="13"
                     /score="88.9"
                     /tool="antismash"
                     /translation="VYLNTAATGLVPAPAVAAMTEAATAFSLGRPGAEDHFPVVEAVRA
                     AFARLVGVPARQVATGGSAAVFCGLVAQSLPEGSEVLCAEGDFSSLVNPFHVRPGLGVR
                     TVPLEEVAGAVRPGTALVAVSAVQSADGRIADLGAITAAARAHGARTLVDISQAAGWLP
                     VDAGAYDFTVCVGYKWLMGPRGTAFLTVPEDLGGLTPVFAGWVAGENPWDSTYGKVSLA
                     RSARRYDENPALLPYAGALGGLRLIEELGTERIAAHDRALAERFRAGLAALGHRPVAAP
                     DSAVVSVPGLGHAAERLAAEGVRVSARAGGLRASFHLYNSATDVDRV"
     misc_feature    7658..7676
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 18.4"
                     /tool="antismash"
     misc_feature    complement(7659..7677)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.55"
                     /tool="antismash"
     gene            7668..8678
                     /locus_tag="IE241_RS22370"
                     /old_locus_tag="GCM10010324_45680"
     CDS             7668..8678
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002233.1"
                     /locus_tag="IE241_RS22370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_45680"
                     /product="hypothetical protein"
                     /protein_id="WP_190023591.1"
                     /transl_table=11
                     /translation="MTSTTTTVPDDHATKRRVLRAVAITACLPYLGLKVAWVAGSHVGI
                     PEGSTLRDAGTGLVVANAITILMDATVVALALLLTRPWGRRVPAWLLTLPMWGAVGLLT
                     PIMYGFPAQLTVRALGGSAAAGSGSGAEKPFLDEWVFGVVYTGFIVQGLALGTLFVLYA
                     RERWGHLWRGSVRDLTVRPLGRGQRAALATAGALAVLPGGVHLMWTCGVSAGLTPEIAR
                     MDRGDFAAGESGYVLYAAATIAGAVLLLSGRALSGRGGRLPLRVPLLLAGVGSAATAAW
                     GGWQLLATLTSLADDGRGPTATMAVAYAVQVIIGLLVLAAGARFFVQRSARAAAL"
     gene            8684..9967
                     /locus_tag="IE241_RS22375"
                     /old_locus_tag="GCM10010324_45690"
     CDS             8684..9967
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003989374.1"
                     /locus_tag="IE241_RS22375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_45690"
                     /product="sensor histidine kinase"
                     /protein_id="WP_229899653.1"
                     /transl_table=11
                     /translation="MTALLRRGADALVGRRARLRWVHLLLGGALLMPYYLLASTLTAVF
                     VRDTNILFPSTLGRQFGVFALALVPAAVTALVPFVRTLESEAARALCGVPEEELATGPG
                     ESRAARGRTALWYTLHLLAGGIVSGMSLATPPFALMLIALPVTGVPGGEDAPYGLGDLA
                     FPWTLLALPAGLAVLLLLTGASAAAGALLARCAPVLLGPTAADRLAEAEQHAMRLALRN
                     RLARELHDSVGHALSAVTLQASAARKVLDADPEFARQALAAIEETTREAVGELDTVLGL
                     LREDGAASAAPAPTLADLDGLLERTRAAGGTVTLAAPAALDGVPPVVSREAYRIVQEGL
                     TNALRHAGRVPVLLRITVHDDAVEVALENPVNPDKAPGRAHRRTGGGRGLTGIAERARL
                     LRGSSSSGERDGVWRLAVRLPLSGGAGR"
ORIGIN
        1 gggggctggg cttcgcggtg cgccgcggcg acaccttccc ggggctgggg ccggactatc
       61 tgcgcctcgc ggtccgggac cgggcgacca cggaccgctt cgcggaggcc ctcgggaaag
      121 cgctcgccga ggggtgagac cgcctctggc cgaggccggg agtgatcgct agggtgccgc
      181 cgtatggcga cctcgcgaat agccccggca gccccgacaa ccccggcagc cgccgaccgg
      241 acggcacggc tggccgtcac cgtgttcccg ctgctcgtcg tcgcggccgg ggcgctgggg
      301 ctcgccgcgc cgtcgcactt caccggctgg gcgccggccg tgccgtacct gctgggcgtc
      361 gtgatgttca ccatgggcct cacgctgacc ggtgacgact tccgcgcggt cgccaagcgg
      421 ccctgggcgg tcgggctcgg cctcgtcgcc cactacgtga tcatgccggg gctgggctgg
      481 ctgatcgcca cggtgctggg gctgccgccg cagctggcgg ccggggtgat cctggtcggc
      541 tgcgcgccga gcgggacggc gtccaacgtg gtcacgtacc tcgcgcgcgg ggacgtggcc
      601 ctgtcggtgt ccgtcgcgac cgtgtcgacg ctcgtcgcgc ccctggtgac accgccgttg
      661 acactgctgc tggcggaccg gttcctggac gtggacgcgg gcgccatgtt caccgacatc
      721 ctcaagaccg tgctcgtgcc ggtggtcggc ggggcgctgg tgcggatggc ggcggggcgg
      781 tggatcggca gggtgctgcg cgccctgccg gcgctctcgg cgctgacgat cgccgtcctc
      841 gtcctcatcg tggtgtcggg cagcgcggcc cgcatcaagg acgcggccgc cctcgtcctc
      901 ctcgccgtcg tcctccacaa cggcctcggc ctggccctcg gatacgcggc gggccgcctg
      961 gcccggctcg gcggtccgtc gagccgggcg atggccttcg aagtcggcat gcagaactcg
     1021 ggcctggcgg cgtcccttgc aacggcccac ttcagccccg cggcggcgct gccggcggcg
     1081 gtcttctcgg tgtggcacaa cgtgtccggc gcggcggtgg cagcctggat gtcaaggcgc
     1141 cctcactcct agcggcgcga agcgccgacc ggaccccggg tgcaccgggc accgccgaac
     1201 tccaccgtcg tggcggatcg ccactgcggc ggaagggggt tgccggcccc aggcccgaac
     1261 acgggctgaa ggccggcaac ccgccaggcc ggcaccgccg gacaccaccg ccgtggcaca
     1321 tcaccgccgc ggcggaggaa gttgccggcc ccggcggtag acgccgaacc ccggcccctg
     1381 agccgacccg accgcaggtc cgcccggcac cgccggacac caccggcgtg gcggatcgcc
     1441 gccgccgggg cggcggaggg agtcgccgca agccgaccgc aggtcacccc gtgcctagcc
     1501 gatcacccgc ccccgcagaa tcacccgctt cggtgagccc aggaccctga cgtccgcccg
     1561 tggatccgtc tcgtagacga ccaggtccgc cggggcgccc tcctccaggc ccgggcggcc
     1621 cagccaggcg cgggccgacc acgtggcggc cgacagggcc gctagcgggg gcaggccggc
     1681 cttgacgagt tcggcgacct cctggccgac caggccgtgg gcgagcgaac cgcccgcgtc
     1741 cgtaccgacg aagaccggga tgcccgcgtc gtacgccgcc cgcaccgtct cgtagcgccg
     1801 ggcgtgcagg cgccgcatgt ggtccgccca gcgcgggaac ttcgcctcgc cgccgtccgc
     1861 gagccgcgga aacgtcgcga tgttgacgag cgtcggcacg atcgcgaccc cgcgctcggc
     1921 gaagagcgga atggtctcct cggtgagccc ggtcgcgtgc tcgatgcagt cgatgcccgc
     1981 ctccacgagc ggcgccagcg actcctccgc gaagcagtgc gcggtcacgc gcgccccgag
     2041 gcggtgcgcc tcggcgatcg cggccgcgac ctccccgcgc ggccagcacg cggacaggtc
     2101 gcccgtctcg cggtcgatcc agtcgccgac gagcttgacc cagccgtccc cgcgccgcgc
     2161 ctcccgcgcc acgtacgcca cgaggtcgtc cggctcgatc tcgtgcgcgt agttgcggat
     2221 gtagcggcgg gtgcgcgcga tgtggcgccc ggcgcggatg atgcggggca ggtcctcgcg
     2281 gtcgtcgatc cagcgcgtgt ccgaggggga gccggcgtcg cgcagcagca gcgctcccgc
     2341 atcccggtcg gtgagcgcct gcttctcggc ggtggaggcg tcgaccgggc cgtgggcgtc
     2401 gaggcccacg tggcagtgcg cgtccacgag gccgggcagc gcccacccct cgacggtggt
     2461 gacgtcccgc gcccccgccg gccgttcgaa ggtgatccgg ccgtcgacgg cccacagctc
     2521 gtcgcgcacc ccgccgtccg ggtccgcgcc gacgagcacc cgccccttga tgtgcagcac
     2581 cccactgtcg ctcatggacc gcactgtacg agcccgctcc tcccgcccgc caaggggctg
     2641 ccggggcgcc gccggggggc ggccgccggg gcgcctccac ggccgccccg ggcaattccc
     2701 cggaactcct ctccggtaat tcctcagaca actctccgcg cattctcatg cccgctttcc
     2761 cgtaccctaa acgcagcatt ttccttacgt tcccgcaagg gaattccccg ccccttaccg
     2821 atccccggcg atcacctgtg acacactcca caccatccgc gatttgcccg gatattaccg
     2881 ggcaaaccac cacatcatcc acccgctttg cgcgcccccg cgcctccgcg cgataacaca
     2941 accaccccct tccgagtaac ccggaccccc gatgcgattt ccgatccgca tcggtaaatt
     3001 ccctccgcat gaccgcagca caagcagacc ttgcacgtgc ccgtcccgcc tccctccggg
     3061 aattcccgga aatgccggag gacctcacgc tcgacgtccc gcgggtcgag gacggagccg
     3121 cgatctggcg catcgcccgc gactcgggaa ccctggacct caactcctcg tacagctacc
     3181 tgctgtggtg ccgcgacttc gccgccacct cggccgtggc ccgccccgcc ggcggcggga
     3241 cgggtgggaa gggcggggag cccgccggat tcatcaccgg ctacgtccgg cccgcccgcc
     3301 cccgcaccct cgtggtctgg caggtggccg tggaccgccc gcaccgcggc cgcggcctgg
     3361 ccgccgccct gctggagggc ctcgtcgccc gggtcgcccg cgagagcgag ggcggcatcg
     3421 acgggatcga gaccaccgtc acgcccggaa acaccgcctc caaccgcctg ttcgcctcct
     3481 tcgccgagcg gcaccgcgcc cccgtcgagc gcgaagtcct cttccacggc gggctgttcc
     3541 ccgagacggg gcacgacccc gaggtactgc accgcatcgg ccccctgacg cactgagacg
     3601 cgctgagacg cacgagacgc acgagacgca ccgaaacgtc cgagaccgct cacacaccga
     3661 caggagcacc cccatgacca tcaccccgcc cgcactcagc gtcttcgaga ccctggagtc
     3721 ggaggtgcgc agctactgcc gcagctggcc cgcggtcttc gaccgcgccc agggcagccg
     3781 gatgtacgac gaggacggcc atacgtacct cgacttcttc gccggtgccg gagcactcaa
     3841 ctacggccac aacaacccgg tcctcaaacg cgccctgatc gactacatcg aacgcgacgg
     3901 cgtcacccac ggcctggaca tggggaccac cgccaagcgc gcgttcctgg aggccttcca
     3961 ggacgtcgtg ctgcgcccgc gcgacctgcc gtacaaggtg atgttccccg gcccgacggg
     4021 caccaacgcc gtggaggccg cgctgaagct ggcccgcaag gtcaagggcc gcgaggcgat
     4081 cgtgtccttc accaacgcct tccacggcat gtccctgggc tcgctcgccg tcaccggcaa
     4141 cgccttcaag cgggccggtg cgggcatccc cctggtgcac ggcaccccga tgcccttcga
     4201 ccactacctc gacgaccggg tgccggactt cctgtggttc gagcggctgc tggccgacca
     4261 gggctcgggc ctgaacaccc ccgccgccgt catcgtcgag acggtgcagg gcgagggcgg
     4321 catcaacgtg gcccgccccg agtggctgcg cgggctcgcc gcgctgtgcg agcgccacga
     4381 catgctgctg atcgtggacg acatccagat gggctgcggc cgcaccggct ccttcttctc
     4441 cttcgaggag gcgggcatca ccccggacat cgtgacgctc tcgaagtcca tcggcggata
     4501 cggcctgccg atgtcgctca ccctcttccg cccggagctc gacctctggg agcccggcga
     4561 gcacaacggc acgttccgcg gcaacaaccc ctccttcgtc accgccgccg cggcgctcga
     4621 cgcgtactgg agggacgggc agatggagaa gcagaccctc gcccgcggcg agcaggccga
     4681 ggaggcgctg cgcgccatct gcaccgagca cccggagctg ggcggccact accggggccg
     4741 cgggctcgtg tgggggctgg agttcgccga caaggcccgg gcctcggcgg tcgccgggcg
     4801 ggccttcgag ctcggcctgc tcgtggagac gtcgggcccg gagggcgagg tcgtcaagct
     4861 gctgcccgcc ctgacgatct cccccgagga gctggacgag ggcctgcgga tcctcgcccg
     4921 cgcggtccgc gagacggcct gaccgcacca ccaccgctgg taccgccact ggtaccgcca
     4981 ccgaaagaaa ggaaccaccc gtgatcgtcc gttccctcca ggacatcgag aacaccgacc
     5041 gccacgtcaa ggccaagtcc ggtacgtggg agagcaagcg gatcgtgctc gccaaggagc
     5101 gggtcggctt ctccctgcac gagacggtcc tctacgccgg cacggagacg tccatgtggt
     5161 acgccaacca catcgaggcc gtgctgtgcg tggagggcga ggccgagctc accgacgacg
     5221 agaccggcga gaagcactgg atctcccccg gcacgatgta cctgctcgac ggccacgagc
     5281 ggcacaccct gcgcccgaag accgacttcc gctgcgtgtg cgtcttcaac ccgccggtga
     5341 cgggacggga ggaccacgac gagaacggcg tctacccgct gctgacggag gagggctgaa
     5401 ccatggccgc ggtcaccgac ctctacccca cgcgcggcac gcacgaggcg gccgtcccgc
     5461 gccgtgaccc cgtggtgtgg cccgggtccg gatccgcgtc cgcgtccggg tccggctcgc
     5521 gcacgcccgg gccgatcgac gcggccgccc tcgcgggcta cgaacgcgac gggttcctca
     5581 ccgtggacga gatgatcacg ccgggcgagg tgcaggtcct gcgcgccgag ctggaccggc
     5641 tgatcgccga cccggcggtg cgggccgacg agagagcggt cgtggagccg cactcgcagg
     5701 acgtgcggtc ggtgttcgaa gtgcaccgga tcagcgacgt gttcgcgcgg ctggtacgcg
     5761 atccgcgcgt ggtgggccgg gcgcggcaga tcctcggctc cgacgtctac gtccaccagt
     5821 cgcgggtcaa cgtcaagccg ggcttcgggg cctccgggtt ctactggcac tcggacttcg
     5881 agacctggca cgcggaggac gggctgccgc ggatgcggac ggtgtcggtg tcgatcgcgc
     5941 tgacggagaa cctcgacacc aacggcggcc tgatgatcat gccggggtcg caccggacgt
     6001 tcctgggctg tgcaggggtg acgccgcagg acaactacaa gcggtcgctg cggatgcagg
     6061 aggcgggcac gccgtcggac agcgcgctcg ccgcgctggc gggccggcac ggcatccggc
     6121 tcttcacggg cccggccggt tcggcgacgt ggttcgactg caactgcatg cacgggtcgg
     6181 gcgacaacat cacgccgttc ccgcgcagca acgtcttcat cgtcttcaac agcgtggaga
     6241 acacggcggt ggaccccttc gcggccccgt cccgccgccc ggagttcgtg ggggcacggg
     6301 acttcacgcc ggtggggtga ccggcacggg cggccacggc cacggccatc cgtccgcccg
     6361 gcgaacccgg gccgtggccg ccccttcctt atccgcccct tccttgtccg ccctgctcag
     6421 gacagcgtct tcaagaccct gtccacgtcg gtcgcgctgt tgtagaggtg gaacgacgcc
     6481 cgcagtcccc cggcccgtgc gctgacgcgt acgccctccg ccgcgaggcg ctccgccgcg
     6541 tgtccgaggc cggggaccga cacgacggcg gagtccggcg ccgccacggg ccggtgcccg
     6601 agcgcggcga gccccgcgcg gaagcgctcg gcgagcgccc ggtcgtgcgc ggcgatccgc
     6661 tccgttccga gctcctcgat cagccggagc ccgcccaggg ctcccgcata cggcaggagg
     6721 gcggggttct cgtcgtagcg gcgggcggag cgggcgagcg acaccttccc gtacgtgctg
     6781 tcccaggggt tctcccccgc cacccacccg gcgaagacgg gggtgaggcc gccgaggtcc
     6841 tcggggacgg tgaggaaggc cgtgccgcgg ggccccatca gccacttgta gccgacgcag
     6901 acggtgaagt cgtacgcgcc cgcgtcgacg ggcagccagc ccgcggcctg gctgatgtcg
     6961 acgagcgtgc gggcgccgtg ggcgcgggcc gccgcggtga tcgcgccgag gtcggcgatg
     7021 cggccgtcgg ccgactggac ggcgctgacg gcgacgagcg cggtcccggg gcgcaccgcg
     7081 ccggcgacct cctccagggg gacggtgcgg acgccgagcc cgggccgcac gtggaagggg
     7141 ttgacgaggg agctgaagtc gccttccgcg cacaggactt ccgacccctc gggcagcgac
     7201 tgggcgacga gcccgcagaa gacggcggcc gagcccccgg tggcgacctg ccgggcgggc
     7261 acgccgacga ggcgggcgaa ggcggcgcgg acggcctcca cgaccgggaa gtggtcctcc
     7321 gcgccggggc ggcccagcga gaacgcggtg gcggcctcgg tcatggcggc gacggcgggg
     7381 gcgggcacga gcccggtggc ggcggtgttg aggtagacgg tctcgggcgc gaagtgtgcg
     7441 ggggcgaggc tctccatgcc cactcactat gcgcgtccgg caatcactcg tccatcggcc
     7501 ggcccacggg ccggtccatc ggccggtcca cgggccggtc caccggccgg tccatgcggg
     7561 agcctcccgg cccctgcctg aggacgagcc gcccttccgc cgcgcggcgg aggaggtccg
     7621 ccggtcaggg cgacgcggcg gcgccccacg gacggaacgc tggtcacatg accagtacga
     7681 cgacgaccgt cccggatgac cacgccacca agcgccgcgt gctccgcgcg gtggccatca
     7741 ccgcctgcct gccctacctc ggcctcaagg tcgcctgggt ggcgggcagt cacgtcggca
     7801 tcccggaggg cagcaccctg cgggatgccg gcacggggct ggtcgtggcg aacgccatca
     7861 ccattctgat ggacgccacg gtggtggccc tcgccctgtt gctgacccgc ccctggggca
     7921 gacgcgtccc cgcctggctg ctgacgctgc cgatgtgggg cgcggtgggg ctgctgacgc
     7981 ccatcatgta cggcttcccg gcgcagttga ccgtccgcgc actcggcggt tcggcggcgg
     8041 ccggttccgg cagcggggcc gagaagccgt tcctggacga gtgggtcttc ggcgtggtct
     8101 acaccgggtt catcgtccag gggctggccc tgggcaccct gttcgtgctg tacgcccgcg
     8161 agcgctgggg acacctgtgg cggggcagcg tccgtgacct gacggtccgt ccgctcggcc
     8221 ggggccagcg ggccgcgctg gccacggcgg gcgccctggc cgtcctgccc ggcggggtgc
     8281 acctgatgtg gacgtgcggg gtgtcggcgg ggctcacgcc ggagatagcc cggatggacc
     8341 gcggcgactt cgcggccggg gagtcgggct acgtgctgta cgccgccgcg acgatcgcgg
     8401 gcgccgtcct gctgttgtcc ggccgggccc tgtccggacg cggcggccgg ctaccgctgc
     8461 gcgtgccgct gctcctcgcg ggggtcggct cggccgcgac ggcggcgtgg ggcggctggc
     8521 agctgctcgc gacgctgacg tccctggccg acgacggccg ggggccgacg gcgacgatgg
     8581 ccgtcgccta cgctgtgcag gtgatcatcg gtctgctggt cctcgccgcc ggcgcacgct
     8641 tcttcgtcca gcgctccgcg cgggccgccg cgctgtgagg gccgtgactg ccctcctgcg
     8701 gcgcggcgcc gatgccctgg tgggccgccg cgcccgcctg cgatgggtgc acctgctgct
     8761 cggcggtgcg ctgctgatgc cgtactacct gctggcgagc acgctgacgg cggtcttcgt
     8821 cagggacacg aacatcctgt tcccgtccac cctcggccgg cagttcggcg tcttcgcgct
     8881 ggcgctggtg ccggcagcgg tgaccgcgct ggtgcccttc gtacggacgc tggagtcgga
     8941 ggcggcacgc gccctgtgcg gggtgccgga ggaggagctc gcgacgggcc cgggcgagtc
     9001 gcgggccgcg cgcgggcgga ccgccctgtg gtacaccctg cacctgctgg ccggcggcat
     9061 cgtgagcggc atgtcgctgg ccacgccccc gttcgcgctg atgctgatcg ccctgccggt
     9121 caccggcgtc ccgggaggcg aggacgcccc gtacgggctc ggggacctgg ccttcccgtg
     9181 gacgctgctg gcgctgccgg ccggcctggc ggtgctgctc ctgctcaccg gggcgtccgc
     9241 ggccgcgggc gccctcctcg cccgctgcgc gcccgtcctg ctcggcccga ccgccgccga
     9301 ccggctggcg gaggccgagc agcacgccat gcggctggcc ctgcgcaacc ggctggcgcg
     9361 cgaactgcac gactccgtgg ggcacgcgct gagcgcggtc acgctgcagg cgagcgccgc
     9421 gcgcaaggtc ctcgacgccg acccggagtt cgcgcggcag gcgctggccg cgatcgagga
     9481 gacgacgcgc gaggcggtcg gcgagctcga caccgtcctc gggctgctgc gcgaggacgg
     9541 cgccgcctcc gccgcgcccg cgccgaccct cgccgacctg gacgggctgc tggagcgcac
     9601 ccgcgccgcg ggcggcaccg tcaccctcgc cgcaccggcc gccctggacg gcgtgccgcc
     9661 ggtcgtctcc cgcgaggcgt accggatcgt gcaggagggc ctcaccaacg ccttgcggca
     9721 cgcgggccgg gtgcccgtgc tgctgcgcat caccgtgcac gacgacgccg tggaggtcgc
     9781 cttggagaat ccggtcaacc ccgacaaggc ccccggccgc gcgcaccgcc gcaccggcgg
     9841 cggccgcggt ctgacgggca tagccgaacg ggcccggctg ctgcgcggca gcagcagctc
     9901 cggcgagcgg gacggggtgt ggcggctcgc cgtccgcctg ccgctgagcg ggggtgcggg
     9961 ccggtgacga cgcccctgcg gatcgtgctc gccgacgacg agcgcatggt gcggaccgcc
    10021 ctgcgggcga tcctgggcgc cgaggaggac ctggaggtcg tcggcgaggc cacgaccggc
    10081 gcggaggcgg tctccgtcgt ccgcgaactg cgtccggacg tcgtcctgat ggacgtacgg
    10141 atgcccgagg tcgacggcat ccgggccacc gaacagctgc tgggcggcat ggactccccg
    10201 ccgcgcgtca tcgtcgtcac cacgttcgag aacgacagct acgtgtacga cgcgctgcgc
    10261 gcgggcgcca gcggcttcct cctcaagcgg gcgggcgccg acgaactcgt ccaggccgtc
    10321 cggctggtgg cccgcagcga ctcgctgctc ttccccgccg cggtacgcgc cctcgcggcg
    10381 gagcacgcgc agacgcggc
//
