LOCUS       NZ_BMUT01000007         9905 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces hiroshimensis strain JCM 4586 sequence07, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUT01000007
VERSION     NZ_BMUT01000007.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces hiroshimensis
  ORGANISM  Streptomyces hiroshimensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 412151)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 90X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650335.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:32:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,830
            CDSs (total)                      :: 6,745
            Genes (coding)                    :: 6,620
            CDSs (with protein)               :: 6,620
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 25 of 125
            Pseudo Genes (incomplete)         :: 107 of 125
            Pseudo Genes (internal stop)      :: 4 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:07:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9905
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUT01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..9905
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..9905
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..9905
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[3663:4905](+)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3664..4905
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(<1..239)
                     /locus_tag="IE241_RS16980"
     CDS             complement(<1..239)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018510407.1"
                     /locus_tag="IE241_RS16980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="WP_308433712.1"
                     /transl_table=11
                     /translation="MRKVLIANRGEIAVRVARACRDAGIASVAVYADPDRDALHVRAAD
                     EAYALGGDTPATSYLDVAKVLAAAAESGADAIHPG"
     misc_feature    complement(198..209)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.68"
                     /tool="antismash"
     gene            complement(391..1179)
                     /locus_tag="IE241_RS16985"
                     /old_locus_tag="GCM10010324_34790"
     CDS             complement(391..1179)
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007268980.1"
                     /locus_tag="IE241_RS16985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_34790"
                     /product="response regulator transcription factor"
                     /protein_id="WP_190022606.1"
                     /transl_table=11
                     /translation="MNQQVGTLLDAHPGEGPGGRVPRPRAEHPADRSPALARLGETLRI
                     LVIESDAHVGETLTRRLRRHGYETFSVATGEAALDEYRSADLVLLDPELPDLDGLEVCR
                     AIRAASEVPIIAVSAVAGELDRVLGLQAGADDYVVEPYGFRELTARIEAVMRRARPAVR
                     PTAQVITSGPLTIDPDLREVRLGGRRIDVTRKEFDLLHLLASRPEKVVSRKQLMAQVWE
                     DSWSRPGRTIDTHVSSLRGKLGSADWIITVRGVGFRFGQA"
     misc_feature    1215..1228
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: weak, score: 19.59"
                     /tool="antismash"
     misc_feature    complement(1427..1441)
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 16.91"
                     /tool="antismash"
     misc_feature    1505..1523
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.3"
                     /tool="antismash"
     gene            1570..2247
                     /locus_tag="IE241_RS16990"
                     /old_locus_tag="GCM10010324_34800"
     CDS             1570..2247
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985133.1"
                     /locus_tag="IE241_RS16990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_34800"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_190022607.1"
                     /transl_table=11
                     /translation="MTKQERAMNTRHALIRSAAQAFDQFGYTRAKLAGISAGAGVSSGA
                     LHFHFDTKAAMAAAVESEAARTLHALTRHAHRKSPSPLQALIDSSHVIAQQLRWDVVVR
                     AGFHLNCRTAHHSETNLRQEWLECVRHTLERSATHGQTPPQAVREELTAAVVGATAGFE
                     ALARHDRTWLSRHTLTGLWHTLLPQLATPKALAQLQPAGTESVIHATGPTADRPAAAHA
                     LRG"
     gene            complement(2765..3442)
                     /locus_tag="IE241_RS16995"
                     /old_locus_tag="GCM10010324_34810"
     CDS             complement(2765..3442)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010032559.1"
                     /locus_tag="IE241_RS16995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_34810"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_190022608.1"
                     /transl_table=11
                     /translation="MAKQARAIHTRRLILEAAGEVFDEYGYESATIAEILGRAGVTKGA
                     LYFHFASKEELARGVLSEALTTEGVMPQESRLQEWVDVGMVLAHRLPREPLLSAAIRLS
                     ADRKARDLFGTSWPAWIAFIAGQLAAAKEQGELLPHVDADRIATLFVSAWTGVEVLSTA
                     LDGGDSLEERVALLYDTMLPGVAVPGALRALDTAPDRGTRVWEAHQAQREQERRQETAS
                     AAS"
     gene            3664..4905
                     /locus_tag="IE241_RS17000"
                     /old_locus_tag="GCM10010324_34820"
     CDS             3664..4905
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF041195.1"
                     /locus_tag="IE241_RS17000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_34820"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_190022609.1"
                     /sec_met_domain="AfsA (E-value: 1.3e-16, bitscore: 54.8,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSQILLSPAPRADESTPQPADRSVPCRNIRVQADQIDASGRLHAS
                     AADLSAETTREGSGGSGAGDASGGPGADGGGRVAKELVHLSFDDAVYLTGCEQQDATHY
                     TVSALWPAHTTHRIPDHGTHYETLVVAQTIRQAGLFLAHTSYAAPLDHATLLRTFSYTL
                     NPDAVVDTHRATPLHINITATPTTTRNNRTTALHLRMTITAAGSTSGSTGEPGDTAETV
                     LATGETAFEWIPPAVYRRLRGAYHRPPTTLPPVTPPLPPAQAGRTTADEVVLSPTAQPR
                     TWQLRHPFDNTVLYDHAVDHVPGLVLIEAATQAAYTITEPVGSTGTSSGAAAGTGSCAC
                     DPSAVDDGAYRPQPTTTHTTFHRYAEFDAPITITIDTRTASRTVTRTTVGYQNDEPVFT
                     TTHTAPAPAPAPVA"
     gene            complement(4989..5762)
                     /locus_tag="IE241_RS17005"
                     /old_locus_tag="GCM10010324_34830"
     CDS             complement(4989..5762)
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (11-178). E-value: 1.5e-14.
                     Score: 46.2. Matches aSDomain:
                     nrpspksdomains_IE241_RS17005_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019886500.1"
                     /locus_tag="IE241_RS17005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_34830"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_190022610.1"
                     /sec_met_domain="adh_short (E-value: 9.2e-35, bitscore:
                     114.6, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 5.8e-34, bitscore:
                     112.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEHQGFLAGKCVLVTGASSGIGAAAAKVFAREGAAVVLTARRGE
                     RLEALCEEIRGGGGDAAFVVGDLRDEGDARRAVEFAVERHGRLDAAFNNAGIGWDQEPM
                     HLMKDDAYDAIMDTNVRGVWNVMRHELAAMVESGAGGSIVNNSSVAGMLAIPAAAPYIA
                     SKHAVIGLTKAAAAEYAPHGIRVNTVAPGTTRSEIIAGWFERNPHIEPELHRLTPQART
                     AEPAEIAEAAAWLCSDRASFVTGAVLPVDGGFTMV"
     aSDomain        complement(5229..5729)
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE241_RS17005_PKS_KR.1"
                     /evalue="1.50E-14"
                     /label="IE241_RS17005_PKS_KR.1"
                     /locus_tag="IE241_RS17005"
                     /protein_end="178"
                     /protein_start="11"
                     /score="46.2"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="CVLVTGASSGIGAAAAKVFAREGAAVVLTARRGERLEALCEEIRG
                     GGGDAAFVVGDLRDEGDARRAVEFAVERHGRLDAAFNNAGIGWDQEPMHLMKDDAYDAI
                     MDTNVRGVWNVMRHELAAMVESGAGGSIVNNSSVAGMLAIPAAAPYIASKHAVIGLTKA
                     AAAE"
     CDS_motif       complement(5634..5726)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE241_RS17005_0001"
                     /evalue="8.40E-05"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE241_RS17005"
                     /protein_end="43"
                     /protein_start="12"
                     /score="15.2"
                     /tool="antismash"
                     /translation="VLVTGASSGIGAAAAKVFAREGAAVVLTARR"
     gene            5899..7098
                     /locus_tag="IE241_RS17010"
                     /old_locus_tag="GCM10010324_34840"
     CDS             5899..7098
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014180574.1"
                     /locus_tag="IE241_RS17010"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_34840"
                     /product="FUSC family protein"
                     /protein_id="WP_190022887.1"
                     /transl_table=11
                     /translation="MLVRRGREPAVVQTVRSTAAAVISYVVALELSSEPAPLTAPLTAL
                     LVVQVTLYATLTTGVRRVNSVVAGVLIAIGFSVLVGLSWWSLGLIILASLVIGRFVKAG
                     EFVPEVAISAMLVLGVTQVAETAWDRVLETLIGAVVGMLFNVLLVPPVWVQPAGEAIED
                     LARRMRRLLQDIGEEISGHTPVEKAAARLHAARRLDHDIVQVDASLRQAEDSLRLNPRV
                     KEGLLYRVVLRTGLDALEITAVVLRVTCRTLTDLASHRREESLFPEETAAALREVFTNM
                     ALAVENFAVVITTHVTASAEHAEENLTRELEAARTTRDRAADLLLHQVQEHPRQWQLHG
                     ALLAEIDRILDELDVEKRTERLTEELDRHSREQRERYPRLHRIAQRLRSSRYGRRSTLK
                     "
     misc_feature    complement(7273..7288)
                     /note="TFBS match to CsoR, Copper-responsive repressor,
                     confidence: weak, score: 23.18"
                     /tool="antismash"
     gene            7399..7932
                     /locus_tag="IE241_RS17015"
                     /old_locus_tag="GCM10010324_34850"
     CDS             7399..7932
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE241_RS17015"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010324_34850"
                     /product="hypothetical protein"
                     /protein_id="WP_190022611.1"
                     /transl_table=11
                     /translation="MHWRHLRRRCQKVIGRIPVPDPFSAQDLCERLAAERDRPLRLLAL
                     PTPTVPGTPSGMLLSVETEDFILYDGQTSPLHQEHIIVHEIGHLVCNHRSALDDTRLHR
                     HLDITDPHSVRRVLPRIRYGDEQEQEAEMIATLILEAAGRVPAPTLLSGMLGGLESAMG
                     LRVGRPTGASCSPG"
     gene            7917..9068
                     /locus_tag="IE241_RS17020"
                     /old_locus_tag="GCM10010324_34860"
     CDS             7917..9068
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984949.1"
                     /locus_tag="IE241_RS17020"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_34860"
                     /product="MAB_1171c family putative transporter"
                     /protein_id="WP_190022612.1"
                     /transl_table=11
                     /translation="MLTWIENAGIAALWTAAVVRAPQAVRHREQRPLWFAVVMIALAMS
                     LHLEPVTAVLARIVPGAHWVDLTTHLFSIVDAAAVLWFIHGAAGRHRHTHLLFGTAAAV
                     MTALVLLDVLGPPHARNQIAPSRSTASVPDAYWWVFFAFHLTADTLCGFVCWSHGRHGT
                     PRLLRYGLRLFGTGILLASALWSLKIVYLQTRSPVFAPLFSPVTGAEAAFMALGVALPV
                     FARLRTHLQYRRSYRGLEQLWQDLTTHTPDVVLRPANRIGAAAVPLQLRLYRRVIEIRD
                     AMIVLRNYIPPATLDSIRVRVGGQALPEHLVDAHITACWLTAALQNKHDGLKPHAQTAN
                     LTGPAAGDDARSSARSLTHEIDHLLLVASAYHATTAASPRAGK"
     gene            9096..9425
                     /locus_tag="IE241_RS17025"
                     /old_locus_tag="GCM10010324_34870"
     CDS             9096..9425
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543062.1"
                     /locus_tag="IE241_RS17025"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_34870"
                     /product="thiamine-binding protein"
                     /protein_id="WP_190022613.1"
                     /transl_table=11
                     /translation="MLIAFTLTPVGVGESFRDEIADAVKIIRESGLPHRSDSMYTMIEG
                     EWEEVMDVVRRATEAVAARAPRVQLVLKADLWPGMPGMLDGKISTFEQHVAGRDAAAPA
                     PDGTA"
ORIGIN
        1 ccggggtgaa tggcatccgc ccccgactcg gccgccgcgg caagcacctt ggccacatcg
       61 aggtaactcg tcgccggggt gtcaccgccc agcgcatacg cctcatcagc agcgcggaca
      121 tgcagcgcgt cccggtccgg atccgcgtag accgccacac tggcaatccc ggcatcccgg
      181 cacgcgcgag ctacgcggac agcgatttcg ccacggttgg cgatgagcac cttgcgcacg
      241 atggatgtcc tctttcaaag acgatcggaa gtcaaaagac gatcggaagt catcggatgt
      301 cggtagacct aatcagagaa cggtcgagtg gtgatcgatg aggtcatcgt gtgcggggcg
      361 gcgtcgtcag gccggcggga ggggacgggc tcaggcctgc ccgaagcgga agccgacgcc
      421 gcggacggtg atgatccagt cggcggatcc gagcttgccg cgcaggctgc tgacgtgggt
      481 gtcgatcgtc cggccggggc gggaccagga gtcctcccag acctgggcca tgagctgttt
      541 gcgggagacg accttctccg gccgggaggc cagcaggtgg aggaggtcga attccttgcg
      601 ggtgacgtcg atccggcgtc cgcccaggcg cacttcgcgc aggtcggggt cgatggtcag
      661 cggcccgctg gtgatgacct gcgccgtggg gcgcaccgcg gggcgcgcac ggcgcatgac
      721 ggcttcgata cgggccgtca gctcgcggaa gccgtagggc tcgacgacgt agtcgtcggc
      781 gccggcctgc aggccgagga cccggtcgag ctcaccggcc acggcgctca ccgcgatgat
      841 ggggacctcg ctggcggccc tgatggcccg gcagacctcg aggccgtcca ggtcgggcag
      901 ttccggatcc agcaggacga ggtccgccga acggtactcg tcgagggccg cctccccggt
      961 ggcgaccgag aaggtctcat agccgtggcg ccgcagtctt cgggtgagtg tctcaccgac
     1021 gtgcgcgtcg ctttcgatga cgaggatccg cagggtctca cccagccgcg ccagggcagg
     1081 ggatcggtcc gcggggtgtt ccgccctcgg ccgcggcaca cggcccccag gaccctcgcc
     1141 cgggtgcgcg tcgaggagag tgccgacctg ctgattcatc gctgctcctg cacgtccgcg
     1201 cggcgacggt gccgcgaaca accgttcact gtgagagtac aaaccggact gtcggtttgt
     1261 caacgggcta tagggcaagg cgatgcctcc acccacagaa gcctcatatc ggtcatcaac
     1321 ggatggcgcc gaccgggaat ccgggtcaac ttgcctcaca aaaagagggt ttatgagcac
     1381 tcacaagagg ggaacccgac cgccaccacc tcgccggtcg ccacagtacg caacatgaga
     1441 ggtacaggcc gtgcgggacc ttcgagcgcc agatactcca cataaggagc atctcgggcg
     1501 gcgacagtag aaataaaacc agttagttgg tttcatgttg ctcatgacgt cgtcctccag
     1561 gagttgccgg tgacgaagca agaacgggcc atgaacaccc ggcacgcact gatccgctcc
     1621 gcagcccagg ccttcgatca gttcggctac acccgcgcca agctcgccgg catcagcgcc
     1681 ggcgccgggg tgagctcggg cgccctgcac ttccacttcg acaccaaggc cgccatggcc
     1741 gcagcggtgg agtccgaggc cgcccggacc ctgcacgccc tgacccggca cgcccaccgc
     1801 aagagcccct cccccctgca ggcgctcatc gactcgtcgc acgtcatcgc ccagcagctg
     1861 cggtgggacg tcgtggtgcg cgcaggcttt cacctcaact gccgtaccgc acaccactcc
     1921 gagacgaatc tgcgccagga atggctcgag tgcgtacgac acacgctgga gcggtcggcg
     1981 acccacgggc agacgccgcc gcaggccgtg cgcgaggagc tgaccgccgc ggtcgttggc
     2041 gcgaccgccg gattcgaggc cttggcgcgt cacgaccgca cctggctctc acgtcacacc
     2101 ctgaccggcc tctggcacac tctgctgccg cagctggcca cacccaaggc cctggcccaa
     2161 ctgcagcccg ccggcacgga gtccgtcatc cacgcaaccg gccccactgc cgaccggccc
     2221 gccgcggccc acgcgctccg cggctgagct cccgcagcac cctcccgaaa aagagaccgg
     2281 cggccacggc accgccacca caaggcccgc aatgtccgtt gcgggccttt gccgtgcccg
     2341 gaaagacaca gagaaaccaa ccatgcggtt tctcacgcag acgagctcga tcgccgcccc
     2401 ggccagacat tcgcccaggt cactcgatac gtatgggatt cccgtcgacc cttgacaaac
     2461 aggatggccg gtttgttagc gtccctgacg tcggatcacc cgagggcctc actgtcacga
     2521 gcagcggctc cgggacagct tttcgatgcc gcgcaggggt aacgaggagg gctcgtaccc
     2581 atggcaccac gcgcccgcgc cggcatcggc cataggacac agggggaaag cgtggacgtc
     2641 gtcacccgtg gcagggcgga gggctcgcca ctccatgcgg cagcggtcct gcacagcccg
     2701 ccgcccgcac atcttctacg cgttctacgc gcccggcgtc accgccgggc cactacttcg
     2761 gtcgtcagga ggcggcggat gccgtttcct gacggcgttc ctgctcgcgc tgggcctggt
     2821 gcgcctccca gaccctcgtc ccccggtccg gggccgtgtc cagcgcccgc agtgcacccg
     2881 gcaccgcgac acccggcagc atcgtgtcgt agagcagcgc gacccgctcc tccagggagt
     2941 cgcccccgtc gagtgccgtc gacaggacct cgacgcccgt ccaggcgctc acgaacagcg
     3001 tggcgatccg gtccgcgtcc acgtggggca gcagctcccc ctgctccttc gccgcggcca
     3061 gctgtcccgc gatgaaggcg atccacgccg gccaggacgt gccgaagagg tcccgcgcct
     3121 tgcggtcggc cgacagccgg atcgcagcgc tcagcagcgg ctcgcgcggc agccggtgcg
     3181 ccagcaccat gccgacgtcc acccactcct gcaggcgcga ctcctggggc atgacgcctt
     3241 cggtggtcag agcctcactg aggacgccgc gcgccagctc ctccttggag gcgaagtgga
     3301 aatacagcgc ccccttggtc acgcccgcgc gtccgaggat ctccgcgatc gtcgccgact
     3361 cgtacccgta ttcgtcgaag acctcccctg ccgcctcaag gatgagacgg cgcgtatgaa
     3421 tcgcccgtgc ctgcttcgcc attgattacc tcccgatcac cgacagtcaa ccgccacaag
     3481 accgctcacc cggcgacatc cacagtccgc cccggtgcgg cgaatccggg gtcttcctca
     3541 cccggcccac cgggtgaccg gccctcatac cctgccaccc acccaaaagc aaaccggata
     3601 gtacgtatct tatcaccgag ccggctaacc accgggacgc acgaatccac tccgaggaga
     3661 gacgtgtctc agatcctgct cagccccgct ccccgtgccg acgagagcac cccgcagccc
     3721 gccgaccggt ccgttccgtg ccggaacatc cgagtgcagg ccgatcagat cgacgcgtcc
     3781 ggccggctcc acgcctcagc cgcagacctt agcgccgaga ccacgcggga gggcagcggc
     3841 ggcagcggcg ccggcgacgc ctccggcggc cccggagccg acggcggcgg ccgcgtggcc
     3901 aaggagctgg tccacctgag cttcgacgac gccgtgtacc tcaccggctg cgaacagcag
     3961 gatgccaccc actacacggt ctccgcgctc tggcccgccc acacgaccca ccgcataccg
     4021 gaccacggca cccactacga gaccctcgtg gtcgcccaga ccattcgcca ggcgggcctc
     4081 ttcctcgccc acacctctta cgcggcaccc ctggaccacg ccactctcct gcgcaccttc
     4141 tcctacaccc tcaacccgga tgccgtcgtc gacacccacc gggcgacacc gctgcacatc
     4201 aacatcacgg ccacccccac cacaacgcgc aacaaccgca ccaccgccct ccacctccgc
     4261 atgaccatca ccgctgccgg gtccacctcc gggtccaccg gcgagcccgg cgacaccgcc
     4321 gaaaccgttc tcgccaccgg cgagacggcc ttcgagtgga ttcccccggc ggtctaccgc
     4381 cgtctgcggg gtgcgtacca ccggccgccc acgactctgc cgcccgtcac accgccgctc
     4441 ccgcccgcgc aggccggtcg caccacggcc gacgaggtcg tgctcagccc caccgcgcag
     4501 ccccgcacgt ggcagctgcg ccaccccttc gacaacaccg tcctctacga ccacgccgtc
     4561 gaccacgtcc ccggtctcgt gctcatcgag gcggccaccc aggccgcgta caccatcacc
     4621 gagcccgtcg gcagcaccgg cacgtcctca ggtgccgccg ccggcaccgg ctcgtgcgcc
     4681 tgcgacccgt ccgctgtgga cgacggcgcc taccgtccgc agcccaccac gacgcacacc
     4741 acgttccacc ggtacgcgga gttcgacgcc cccatcacca tcacgatcga cacccgcacg
     4801 gcttcgcgca ccgtcacccg cacgacggtc gggtaccaga acgacgagcc cgtcttcacc
     4861 accacgcaca ccgctcccgc gcccgcgcct gctcccgtcg cctgacgggc cgggcggacc
     4921 acagcgcaac acagcggaac acgggcggag cccggcggcc ggaccgggcc gccgggctcc
     4981 gcgccgcgtc acaccatcgt gaatccgccg tccaccggca ggaccgcgcc tgtcacgaac
     5041 gacgcccggt cgctgcacag ccaggccgcg gcctccgcga tctcggccgg ctcggccgtg
     5101 cgcgcctgcg gcgtcaggcg gtggagctcg ggctcgatgt gcgggttccg ctcgaaccag
     5161 ccggcgatga tctcgctccg cgtcgtcccg ggcgcgaccg tgttcacccg gatgccgtgc
     5221 ggcgcgtact ccgccgcagc ggccttcgtc agcccgatca ccgcgtgctt cgacgcgatg
     5281 tacggggcgg cggcgggtat cgccagcatc ccggcgacgc tgctgttgtt gacgatggaa
     5341 ccgcccgccc cggactccac catcgccgcg agctcgtgcc gcatgacgtt ccagacacct
     5401 cgcacgttcg tatccatgat cgcgtcgtag gcgtcgtcct tcatcaggtg catcggctcc
     5461 tggtcccagc cgattcccgc gttgttgaac gcggcgtcga gacgcccgtg ccgctccacc
     5521 gcgaactcca ccgcccgccg cgcgtcaccc tcatctcgta ggtcgcccac gacgaatgcg
     5581 gcgtcaccac cgcccccgcg gatctcctcg cacagcgctt ccaggcgctc gccccggcgt
     5641 gccgtcagca ccaccgcggc cccctcacgc gcaaacacct tcgccgcggc ggcgccgatg
     5701 ccgctgctcg cccccgtcac cagaacacac ttaccggcaa gaaaaccctg atgttcagtc
     5761 atggccggaa tcctgccatc acgcaccggc cgctctcccc cggcccccgc ccgccacccg
     5821 agtggcgcac cggtggctca caggcgggcg ggaaagggaa gggactccgg tgtgcccggg
     5881 cttcgtgacc ccctgacgat gctggtgagg cgcggcaggg agcccgcggt cgtgcagacc
     5941 gtccggtcca ccgcagccgc ggtgatctcc tatgtcgtgg cgctggagct gagcagcgaa
     6001 ccggccccgc tgaccgcgcc gctcaccgcg ctgctggtcg tccaggtcac cctgtacgcc
     6061 acgctcacca caggggtgcg ccgggtcaat tccgtcgtgg ccggcgtcct gatcgcgatc
     6121 ggtttcagcg tgctggtcgg gctgtcctgg tggagcctcg ggctgatcat cctggcctcc
     6181 ctcgtcatcg ggcggttcgt gaaggcgggc gaattcgtcc ccgaggtcgc gatcagcgcg
     6241 atgctcgtcc tcggggtgac gcaggtcgcg gaaacggcgt gggaccgggt gctggagacc
     6301 ctgatcggcg cggtcgtcgg gatgttgttc aacgtcctgc tcgtcccccc ggtatgggtg
     6361 cagccggcag gggaggccat cgaggatctc gcccgccgga tgcgccggct cctgcaggac
     6421 atcggcgagg agatcagcgg gcacaccccc gtggagaagg ccgcggcccg gctgcacgcg
     6481 gcccggcgcc tcgaccacga catcgtccag gtggacgcct cgctgcgcca ggccgaggac
     6541 agcctccgcc tcaatccgcg cgtcaaggag gggctcctct accgggtcgt gctgcgcacc
     6601 gggctggacg cgctcgagat caccgcggtg gtgctgcgcg tgacctgccg gaccctcacc
     6661 gacctcgcga gccaccgcag ggaggaatcg ctcttccccg aagagaccgc cgccgcgctc
     6721 cgggaggtct tcacgaacat ggccctggcg gtcgagaact tcgccgtcgt catcacgaca
     6781 cacgtcaccg ccagcgccga acacgccgag gagaacctca cgcgcgagct ggaggccgcc
     6841 cggaccaccc gtgaccgcgc cgcggacctc ctcctccacc aggtgcagga gcacccgcgt
     6901 caatggcagc tgcacggggc gctcctcgcg gagatcgacc gcatcctcga cgagctcgac
     6961 gtcgagaagc gcaccgagcg gctgacggag gaactcgacc gccactcccg ggagcagcgc
     7021 gagcgctacc cgcgtctcca ccgcatcgcc cagcgcctcc gcagcagccg ctacggccgt
     7081 cgcagcacgc tcaagtagcc gtcgcagcac gctcgaacac ccggtcgcgg cacgctcgaa
     7141 cagccacccg tccgccccta cccggccgct ttcgccggca acgttccgca accctggcgt
     7201 gacgcggcgc ggcgatctac gatcacaggc ggcttgggca tatgtgcgag gggggctcgt
     7261 ggtgctgcca ttgaccgcct tggggtatgc agtgccggcc cgccggcccg ccgcctcctc
     7321 cggggggccg cggtgacccg gcgcggctac cgggtcatgt gcccgcccgt ccctacgacc
     7381 gcgggagaag cgactgagat gcactggagg catctgcgcc gccgatgcca gaaggtgatc
     7441 ggccggattc ctgtgccgga ccctttctcc gcacaggacc tgtgcgaacg cctggccgcc
     7501 gaacgtgacc ggccgctgcg tctactggct ctgcccacac cgaccgtccc cgggacgccg
     7561 tcgggaatgc tgctgtccgt cgagaccgag gacttcatcc tctacgacgg gcagaccagc
     7621 ccgctgcacc aggagcacat catcgtccat gagatcgggc acctggtgtg caaccaccgc
     7681 tccgcgctcg acgacacccg gctccaccgc catctcgaca tcaccgatcc ccattcggtg
     7741 cgccgggtcc tgccgcggat ccgctacggc gacgagcagg agcaggaggc ggagatgatc
     7801 gccaccctga tcctggaggc ggccgggcgg gtgcccgcgc ccacgctgct gtcggggatg
     7861 ctcggcggcc tggagtcggc gatgggcctg cgggtgggcc gtccgacggg ggcgtcgtgc
     7921 tcacctggat agagaacgcg ggcatcgccg ccctgtggac cgcggccgtc gtcagggctc
     7981 cgcaggccgt ccggcaccgc gagcagcggc ccctgtggtt cgccgtcgtc atgatcgccc
     8041 tggcgatgtc cctgcacctc gaacccgtga cggccgtact ggcgcgcatc gtccccgggg
     8101 cgcactgggt ggatctgacg acccatctgt tcagcatcgt cgacgcggcg gcggtcctgt
     8161 ggttcatcca cggggcggcc ggccgccatc gtcacacgca cctgctgttc ggcacggcgg
     8221 ccgccgtcat gaccgccctc gtcctgctgg acgtgctggg gccgccgcac gcgcgcaatc
     8281 agatcgcgcc gtcgcggagc acggcgagtg tgccggacgc ctactggtgg gtcttcttcg
     8341 ccttccacct cacggccgac accttgtgcg gcttcgtgtg ctggagccac ggccggcacg
     8401 gcacgccgcg cctcctgcgc tacgggctgc gcctgttcgg caccggcatc ctgctggctt
     8461 ccgcgctgtg gtcactcaaa atcgtctatc tccagacgcg ttcgccggtg ttcgcgccgc
     8521 tgttctcgcc cgtcaccggg gccgaggcgg cgttcatggc gctcggcgtg gcgctgccgg
     8581 tgttcgcccg gctgcgcacc cacctgcagt accgcaggtc ctaccgcggt ctggaacagc
     8641 tgtggcagga cctgaccacg catacgccgg acgtcgtcct gcggcctgcc aaccgcatcg
     8701 gcgcggcggc cgtccccctt cagctgcgtc tctaccggcg ggtcatcgag atccgcgacg
     8761 ccatgatcgt cctccgtaac tacattcccc cggccaccct cgactccatc cgcgtgcgcg
     8821 tgggcgggca ggcgctgccg gaacacctgg tggacgcgca catcacggcg tgctggctca
     8881 cggcggcgct gcagaacaag cacgacgggc tcaagcccca cgcccagacc gccaacctca
     8941 ccgggcccgc ggccggggac gacgcccggt cctcggcacg cagcctgacc catgagatcg
     9001 accacctgct gctcgtcgcg tccgcctatc acgcgaccac ggccgcctcg ccgcgcgcgg
     9061 ggaaatgaga agaagagaag ggacggcaga acaccgtgct catcgccttc accctgacgc
     9121 ccgtcggcgt cggagagagc tttcgcgacg aaatcgccga cgccgtgaaa atcatccgcg
     9181 aatccgggct gccgcaccgc tcggactcga tgtacaccat gatcgagggc gagtgggagg
     9241 aggtcatgga cgtcgtccgg cgggccacgg aggccgtcgc cgcgagggcg ccgcgggtcc
     9301 agctcgtcct gaaagccgac ctctggccgg ggatgcccgg catgctggac ggaaagatca
     9361 gcacgttcga gcagcacgtg gccggccgcg acgcagcggc tccggcgccg gacggcacgg
     9421 cctgatcggt gcgcgcatgg cgtcgagtgg ccatccgtgt tgcgatggag ggagcagacc
     9481 tgccacgacg ggaggagcga tcatggcacg gaacaagccg ctcaaggccc gcgacatcat
     9541 gacggcgggc gcgaagtgcg tcggccagca cgaatcgctg ctggacgcgg cgaagatgat
     9601 gcgcgagctg cacgtgggcg cgctgcccat atgcggtgac gacgacaggc tccagggcct
     9661 ggtcaccgac cgggacatcg tcgtccagtg cgtggccgac ggcgtggatc cggcgagtgt
     9721 ggaggcgggt tcgctcggcg gagagctgca ctggatcgac gcggacgccc ctgcgacgga
     9781 cgccctgtcg tccatggagc agcaccagat caagcggctg ccggtgatcg acgtgcagaa
     9841 cggacaccgc ctggtcggca tgatctcgga ggccaacctc gcacagaacc tctccgacaa
     9901 gcaga
//
