LOCUS       NZ_BMUT01000005        10366 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces hiroshimensis strain JCM 4586 sequence05, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUT01000005
VERSION     NZ_BMUT01000005.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces hiroshimensis
  ORGANISM  Streptomyces hiroshimensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 439563)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 90X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650335.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:32:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,830
            CDSs (total)                      :: 6,745
            Genes (coding)                    :: 6,620
            CDSs (with protein)               :: 6,620
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 25 of 125
            Pseudo Genes (incomplete)         :: 107 of 125
            Pseudo Genes (internal stop)      :: 4 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:07:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 304659
            Orig. end                         :: 315025
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUT01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10366
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="1"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10366
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10366
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5366](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5366)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     misc_feature    complement(540..555)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: medium, score: 24.73"
                     /tool="antismash"
     misc_feature    1040..1050
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 16.96"
                     /tool="antismash"
     misc_feature    1459..1468
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     misc_feature    complement(1893..1908)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.68"
                     /tool="antismash"
     gene            complement(2141..2767)
                     /locus_tag="IE241_RS14320"
                     /old_locus_tag="GCM10010324_29320"
     CDS             complement(2141..2767)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083427.1"
                     /locus_tag="IE241_RS14320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_29320"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_190022089.1"
                     /transl_table=11
                     /translation="MNTPGTTVKITVLGATGMAGSRIVDEALTRGHAVTAVARRHDEQA
                     PARAGLQRVTADVADPVLPLEGADVVVSALPARVALPLLPELTARAAGRRLLFVGGAGS
                     LRLPSGERVIDQPGFREEWKPEASAHVELLDALRALPEDVDWTFLSPSALFLPGERTGT
                     FRLGGDDLLAGADGTSRISAEDYAVALLDEIGTPQHRRQRFTVGY"
     gene            complement(2965..3504)
                     /locus_tag="IE241_RS14325"
                     /old_locus_tag="GCM10010324_29330"
     CDS             complement(2965..3504)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003563.1"
                     /locus_tag="IE241_RS14325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_29330"
                     /product="hypothetical protein"
                     /protein_id="WP_229899230.1"
                     /transl_table=11
                     /translation="MDLATGWSTSMRKRALPALGLTVATAGVIGAALAMSPATAGAATA
                     SPAGPRSVTIEAVEKGTSVNFVDVAPSDSALGDQLIGTGDLVGADGRKIGTSSFQGIST
                     DAEPRTAEMLTFVYDLGDGNKITTSGAARLNAQGPVFDEQFAITGGTGKYKNARGQVRV
                     LQETVERAKVTFDIQL"
     gene            3628..4050
                     /locus_tag="IE241_RS14330"
                     /old_locus_tag="GCM10010324_29340"
     CDS             3628..4050
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357064.1"
                     /locus_tag="IE241_RS14330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_29340"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_229899232.1"
                     /transl_table=11
                     /translation="MLHDDDVTLYGLFVEAFSRLKPLVHEDLGIPDTWFEVLLRLGRTP
                     GHALRMSDLATAVAFSSGGFTRLADRMEEAGLIRRDPDPDDRRAALAVLTEAGEQALDR
                     ALQQHLAHLRTHVSGRLSTADRRALERILRTLRDAG"
     gene            complement(4102..4962)
                     /locus_tag="IE241_RS14335"
                     /old_locus_tag="GCM10010324_29350"
     CDS             complement(4102..4962)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267950.1"
                     /locus_tag="IE241_RS14335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_29350"
                     /product="tyrosinase family protein"
                     /protein_id="WP_190022091.1"
                     /transl_table=11
                     /translation="MAVRKNQAKLTKAERRDFVEALLELKRRGGYDKYVETHDRFVMSD
                     LDDQERTGHVSPSFLPWHRQFLLDFERDLQSVNPAVTLPYWDFTVNRSLNDPVFAPDFL
                     GGNGRERDAQVMDGPFAYGGGRWPIYFKVDERPFLMREMGRSKTTPQLPTAATVEAALA
                     APYYDEAPWNSTSKAGFRNLVEGWANGNTGPHNTVHRWVGGHMNTGMSPNDPVFWLLHC
                     FIDKLWSDWQRRHPKAPAYLPVEPVKDIVALNQPMKPWSEKGQRVTPADMIDHRKFYSY
                     DTDAA"
     gene            complement(5001..5366)
                     /locus_tag="IE241_RS14340"
                     /old_locus_tag="GCM10010324_29360"
     CDS             complement(5001..5366)
                     /GO_function="GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0042438 - melanin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010982574.1"
                     /locus_tag="IE241_RS14340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_29360"
                     /product="tyrosinase family oxidase copper chaperone"
                     /protein_id="WP_190022092.1"
                     /sec_met_domain="melC (E-value: 1.1e-38, bitscore: 127.1,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDLTRRRVLGRTAAAAAAIVLAGPAVSAYAAESGHDHHGTGSGH
                     RPFDEIYEGRRIEGRLTSGSGGHHDFGIAVFVDGRPLHIMPRGDGWISVINHYEVFASP
                     YEVTRAAVRALRGANLV"
     misc_feature    5381..5394
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.57"
                     /tool="antismash"
     misc_feature    5381..5395
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.75"
                     /tool="antismash"
     misc_feature    complement(5397..5412)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.57"
                     /tool="antismash"
     gene            complement(5617..6642)
                     /locus_tag="IE241_RS14345"
                     /old_locus_tag="GCM10010324_29370"
     CDS             complement(5617..6642)
                     /EC_number="2.7.1.11"
                     /GO_function="GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983263.1"
                     /locus_tag="IE241_RS14345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_29370"
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="WP_190022093.1"
                     /transl_table=11
                     /translation="MRIGVLTAGGDCPGLNAVIRSVVHRAVVGHGDEVIGFEDGFKGLL
                     DGRHRKLDLEAVSGILARGGTILGSSRLERARLREACESSKELAREYGIDVLIPIGGEG
                     TLTAARMLSDAGLPVVGVPKTIDNDISSTDRTFGFDTAVGVATEAIDRLKTTAESHQRV
                     MVVEVMGRHAGWIALESGMAGGAHGICLPERAFEVDDLVKMVEERFARGKKFAVICVAE
                     GAHPAEGSMEYAKGAIDQFGHERFAGIGTKLAAELERRMGKEARPVILGHVQRGGTPTA
                     YDRVLATRFGWHAVEAAHRGDFGKMTALKGTDIKMVPIADAVTELKRVPESRMDEAESV
                     F"
     misc_feature    6858..6867
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.77"
                     /tool="antismash"
     misc_feature    6860..6873
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.39"
                     /tool="antismash"
     gene            6926..9034
                     /gene="pta"
                     /locus_tag="IE241_RS14350"
                     /old_locus_tag="GCM10010324_29380"
     CDS             6926..9034
                     /EC_number="2.3.1.8"
                     /GO_function="GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="pta"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054972.1"
                     /locus_tag="IE241_RS14350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_29380"
                     /product="phosphate acetyltransferase"
                     /protein_id="WP_190022094.1"
                     /transl_table=11
                     /translation="MTRSVYVTGTDRGDGRQVIELGVMELLTRQVDRVGVFRPLVHGDG
                     PDRLFDLLRSRYRLGQSAESGHGMSYAEASALQAERGTDELVSRLVERFHAVARDYEYV
                     LVLGTDYVGSTLPDELALNARLANEFGASVITVVGGQDQTAESVRAEARNAYRAYEALG
                     CDVVALVVNRIAPADREAVGERLSASLPVPCYALPEEAGLSAPTVAQIVRKLDAEVLLG
                     DDAGLARDAKDFVFGGAMLPVFLPALTEGCLVVTPGDRVDLIVGALAAHSAGAPPIAGV
                     LLTLDEKPGPDILALADRLAPGTPVVSVPGGSFPTAAELFAIEGKLDAFSPRKAETALG
                     LFERHVDTAELTNRIAVARSSRVTPMMFEHELIERSRSARRRIVLPEGTEERVLRAADV
                     VLRRDICDLTLLGEEGAIRKRAGELGIDLAQAQIIDPQTSPLRERFAEIYAKARAHKGM
                     TVELAHDVVTDVSYFGTLMVQEGLADGMVSGAVHSTAATIRPAFEIIKTAPGAQIVSSV
                     FFMCLADRVLVYGDCAVNPDPNAEQLADIAIQSAATAAQFGVEPRIAMLSYSTGTSGSG
                     ADVDKVRKATEIVRERRPDLKVEGPIQYDAAVDAAVAKTKLPGSEVAGKATVLIFPDLN
                     TGNNTYKAVQRSAGAVAVGPVLQGLRKPVNDLSRGALVQDIVTTVAISAIQAQGEAGNG
                     TAPDAPAV"
     assembly_gap    9071..9084
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9144..10355
                     /locus_tag="IE241_RS14355"
                     /old_locus_tag="GCM10010324_29390"
     CDS             9144..10355
                     /EC_number="2.7.2.1"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008776 - acetate kinase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /GO_process="GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018087333.1"
                     /locus_tag="IE241_RS14355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_29390"
                     /product="acetate kinase"
                     /protein_id="WP_190022095.1"
                     /transl_table=11
                     /translation="MTANPTRVLVLNSGSSSVKYQLLDMADGARLAVGLVERIGEETSR
                     LAHTPLATGGGKRETEAPIADHEAALQAVAAELAADGLGLDSPELAAIGHRVVHGGQKF
                     TAPTVITDEVLAEIERLVPVAPLHNPANITGIRTAQALRPDLPQVAVFDTAFHTTMPEA
                     AARYAIDVATADAHMVRRYGFHGTSHAYVSRETAKLLGKDPSEVNVIVLHLGNGASASA
                     VRGGVCVDTSMGLTPLEGLVMGTRSGDLDPAVILHLSRNAGMSTDEIDALLNKKSGLIG
                     LCGDNDMREIRRRIDEGDERAALAFDIYVHRLKKYIGAYYAVLGKVDAVAFTAGVGENA
                     APVREAAVAGLEQLGLAVDAELNAVRSGEPRLISPGSARVAVAVVPTDEELEIATQTYD
                     LVSA"
ORIGIN
        1 ccccaaggtg agcgcgggcg gcagcctcgc cctgttcgac accgcgaccg cgcagaaact
       61 cctcggcacc ccggggcagt tcagcgagct catcgtcgcc gcgaagcccg gcaccgacca
      121 gcagaagctg accgccaagg tccgcgagac gcttcccaag gacgccttcc ggacgcagag
      181 cggaaaggag ctggcggccg aggagtcgaa gatgatcgac aagcagacca gctcgatccg
      241 caacaccttc ctggcgttcg cgggcatcgc cctcttcgtc ggcgtcttca tcatcgccaa
      301 caccttcacc atgctcatcg cccagcgcag ccgggagatc gccctgctcc gcgccgtggg
      361 cgcgtcccgc cgccaggtgg tgcgctcggt gctgatcgag gccgggctgc tcggcctggt
      421 cgcctccgcc gtcggcttcg tcctgggcct cggcgtcgcc accggcctgc gcgcggtgct
      481 gaactccgcc ggggcgagcc tgcccgacgg gccgctggtc atcgagccgg tctccgtgct
      541 gctcgccctc ggcgtcggcg tcgtggtgac cgtgctcgcc gcctggctgc cctcccgcaa
      601 ggccgcgaag atcgcccccg tggaggcgct ggccgccgcg gagcagccgc cggccccccg
      661 cggcctgctg ctgcgcaact ccttcggggc cgtgctgacc gccgccggtg tcgcgatcat
      721 gctgtacgtc accacgctga aggacagcgg cggcctggag atcgccatgg ccggcgccgc
      781 gctgaccatg gccggcgtca tcgtcctggc cccgctgctc tcgcgcccgt tcgtccacct
      841 cttcgcgaag gcgacgggcc ggctgaccgg cgtcagcggc aagctcgcca aggagaacgc
      901 gctgcgcaac ccgcgccgca ccgccgcgac cgcctccgcg ctgatgatcg gcctctccct
      961 gatcaccggt ctgaccgtgg tcgccgtctc cgccgaccag ggcgtcgcga agatgggcca
     1021 gcaggacctg acggccgact acaaggtcac gtcggcgaac ttcggcggcc tctccacgga
     1081 catcgccaag accctcgcca aggaacccgg cgtcaccgcc gtggtcccgc tgcgctcggc
     1141 cggcttccag tcgtcgaacg accccgcgct cgcctacgcc gcggacgtcg cgtcgctcgg
     1201 caaggtggcg gacatccggt tcgtcagcgg ctcccccgcc gatgtgcgga agggcaccgt
     1261 cgcggtgtcc gaccagtacg cccgggagcg cggcctgaag gtcgggtcga ccctgccgat
     1321 ggacttctac gacggcgaga aggccacccc gaaggtcgcc gcgatctacg ccaagaacga
     1381 cgtcgtctcc gccgtcctcg ccaccccctc cctcgtggac ccgcacctgg agaaggtgcg
     1441 tgacgacgag gtgctggtga aggcggcccc cggcaaggcc ggcgagctcc gcgccaagat
     1501 caaggagacc ctcggcggca acccgctcat caaggtgcag agccaggagg acctgcgccg
     1561 ctcggaggcc gggaacatcg acgacatcct cttcctgatg tacgggctgc tgggcatggc
     1621 cgtcgtgatc gcggtcgtcg gcgtcgtcaa caccctcgcc atgtcggtct tcgagcggac
     1681 ccgggagatc gggatgctgc gcgcgatcgg tctggcccgc acgggcgtca agcagatggt
     1741 ccggctggag tccgtgatga tcgcgatgtt cggcgcggtg ctgggcgtcg gggtcggcat
     1801 cttcctcgcc tggtccggcg gccacctggt ccgctcgtcc ttcccgcagt acgagatggt
     1861 cgtgccgtgg gcgcggatcg cggtgttcct gctgatcgcc ctcgccgtgg gcgtcctggc
     1921 cgcggtctgg ccggcccgca gggcggcgaa gctcaacatg ctggagtcga tcggggcgca
     1981 gtagaacgac ggggcgagcc gccccgtcag ccccgcggga gcgccccgtc ccgcggagcg
     2041 acccccgctt cgccgaaccc ccgccggcct ccgacaaggc cggcgggggt tcacgcgtgc
     2101 cgtgccgcct cgccccgctc aggccccctc gccgtcccgc tcagtagccg acggtgaagc
     2161 gctgccgccg gtgctgcggc gtcccgatct cgtccagcag ggcgaccgcg tagtcctcgg
     2221 ccgagatgcg cgaggtgccg tcggcgccgg ccagcaggtc gtcaccgccg aggcggaagg
     2281 tgccggtgcg ctcgccgggc aggaagagcg cggaggggct gaggaaggtc cagtccacgt
     2341 cctccggcag ggcgcgcagt gcgtccagca gctcgacgtg cgcggacgcc tcgggcttcc
     2401 actcctcgcg gaagccgggc tggtcgatga cgcgctcgcc ggacggcagg cgcaggctgc
     2461 cggcgccgcc gacgaagagc aggcggcggc ccgccgcgcg ggcggtcagc tcggggagca
     2521 gcggcagggc gacgcgggcg ggcagcgcgc tgacgacgac gtccgcgccc tccagcggca
     2581 ggacggggtc ggccacgtcg gcggtcacgc gctgcaggcc cgcgcgggcc ggggcctgct
     2641 cgtcgtggcg gcgggcgacg gcggtgacgg cgtggccgcg ggtgagcgcc tcgtccacga
     2701 tgcggctgcc ggccatgccg gtggcgccga ggaccgtgat cttgacggtg gtgccggggg
     2761 tgttcatacg gggttctccc aggggttcgg ggcggctgtc gggcggctgc cggctgctgc
     2821 cgggtgctgc cgggtgctgc cgggtgctgc cgggtgctgc cggcggggcg accggccggc
     2881 aggtcgcggc ggtcacggcc gggtgcgggc agcagtcacg gccgggtgcg ggcggcgggc
     2941 ccgcgcgtca cggccgggac gcggtcacag ctggatgtcg aaggtgacct tcgcccgctc
     3001 cacggtctcc tgcaggacgc gcacctggcc gcgggcgttc ttgtacttgc cggtgccgcc
     3061 ggtgatggcg aactgctcgt cgaagaccgg gccctgggcg ttcagccggg cggcgccgga
     3121 cgtggtgatc ttgttgccgt cgccgaggtc gtagacgaag gtcagcatct cggcggtgcg
     3181 cggctcggca tcggtggaga tgccctggaa gctgctggtg ccgatcttgc ggccgtcggc
     3241 gccgacgagg tcgccggtgc cgatcagctg gtcgccgagg gccgagtcgg acggggcgac
     3301 gtcgacgaag ttgaccgagg tgcccttctc gacggcctcg atggtgacag agcgcgggcc
     3361 ggccggggag gccgtggcgg caccggccgt ggcggggctc atggcgaggg cggcgccgat
     3421 gacaccggcc gtcgcaacgg tgagacccag ggcgggaagg gcgcgcttgc gcatggacgt
     3481 actccagcct gtcgcgagat ccatgatgat ccatggcgga cgaaatatct gccgcagcag
     3541 acaatatgtc tgctgcggca gatactgcaa gagaaggttc tgccggtacg ggcgatggtc
     3601 tgcgaccctg gtgaggtgaa ggagcacatg ctgcatgacg acgacgtgac gctgtacgga
     3661 ctgttcgtgg aggcgttcag ccgcctgaag ccactggtcc acgaggacct cgggatcccg
     3721 gacacctggt tcgaggtgct gctgcggctg ggccgcacgc ccgggcacgc cctgcggatg
     3781 agcgatctgg cgaccgcggt cgccttcagc tccggcgggt tcacgcggct cgcggaccgc
     3841 atggaggagg cggggctgat ccgccgcgac cccgacccgg acgaccggcg ggccgcgctc
     3901 gcggtactga cggaagccgg cgagcaggcc ctggaccgcg cgctccagca gcacctggcg
     3961 cacctgcgca cgcacgtcag cggccgcctc tccacggcgg accggcgcgc gctcgaacgg
     4021 atcctgcgga cgctgcgcga cgccggatag cgacgagggc ggaccggttg cccggcccgc
     4081 cctctcgcga caacggctac gtcacgcggc gtcggtgtcg tagctgtaga acttcctgtg
     4141 gtcgatcatg tcggcggggg tgacccgctg ccccttctcg ctccacggct tcatcggctg
     4201 gttcagggcg acgatgtcct tgaccggctc gaccggcagg taggcgggcg ccttcgggtg
     4261 acggcgctgc cagtcggacc acagcttgtc gatgaagcag tgcagcagcc agaacaccgg
     4321 gtcgttgggc gacataccgg tgttcatgtg gccgccgacc cagcggtgca cggtgttgtg
     4381 cggcccggtg ttgccgttcg cccagccctc gacgaggttg cggaagcccg ccttggaggt
     4441 ggagttccag ggcgcctcgt cgtagtacgg ggcggccagc gcggcctcga ccgtggcggc
     4501 cgtgggcagc tggggcgtgg tcttgctgcg gcccatctcc cgcatcagga aggggcgttc
     4561 gtcgaccttg aagtagatcg gccagcgacc gccgccgtac gcgaacgggc cgtccatgac
     4621 ctgggcgtcg cgctcgcgcc cgttgccgcc gaggaagtcg ggggcgaaga ccgggtcgtt
     4681 cagcgagcgg ttgaccgtga agtcccagta cggaagcgtc acggccgggt tgacggactg
     4741 caggtcccgc tcgaagtcga gcaggaactg ccggtgccag ggcaggaacg acggcgagac
     4801 gtggccggtg cgctcctggt cgtccaggtc gctcatcacg aagcggtcgt gggtctcgac
     4861 gtacttgtcg tagccgccgc ggcgcttgag ctccagcagc gcctccacga agtcgcgccg
     4921 ctcggccttg gtgagctttg cctggttctt gcgaactgcc atgagaatgt ggacctttac
     4981 ggaaatggga tcagggtggc tcagacgagg ttggcgccgc gcagcgcccg cacggcggcg
     5041 cgggtcacct cgtacggcga cgcgaagacc tcgtagtggt tgatcacgct gatccagccg
     5101 tcgccgcgcg gcatgatgtg cagcggccgg ccgtcgacga agacggcgat gccgaagtcg
     5161 tggtggccgc cgctgccgga ggtgagccgg ccctcgatgc ggcggccctc gtagatctcg
     5221 tcgaacggcc ggtggccgga gccggtgccg tggtggtcgt gcccgctctc ggcggcgtag
     5281 gccgatacgg ccggtccggc gagcacgatg gcggcggcag cggcggcggt acggccgagc
     5341 acgcggcggc gggtgaggtc ggacacccgg gtcccccttc agaagaaacg ttccttccga
     5401 ccaggaggtc cagcccgtcg ggctcccccg accccccgac cggcgtaatg atcatgaact
     5461 taacagatct tcgggaagag ccggacatgt ggcgagatag agccaatatg cggctgcata
     5521 ccgctgcata cgactgcata cgaaagaggc cccgccgacg gtgtcggcgg ggcctcttcg
     5581 aagctctcgg aaactctcag gagcggggag ggagggtcag aagaccgact ccgcctcgtc
     5641 catccggctc tccggtacgc gcttgagctc ggtgacggcg tcggcgatcg gcaccatctt
     5701 gatgtcggtg cccttcaggg ccgtcatctt gccgaagtcg ccgcggtggg cggcctcgac
     5761 ggcgtgccag ccgaagcggg tggcgaggac gcggtcgtac gcggtggggg tgccgccgcg
     5821 ctggacgtgg ccgaggatga ccgggcgggc ctccttgccc atgcgccgct ccagctcggc
     5881 ggcgagcttg gtgccgatgc cggcgaagcg ctcgtggccg aactggtcta tggcgccctt
     5941 ggcgtactcc atcgacccct cggcggggtg cgcgccctcg gcgacacaga tgacggcgaa
     6001 cttcttgccg cgggcgaaac gctcctcgac catcttcacg aggtcgtcga cctcgaaggc
     6061 ccgctcgggc aggcagatgc cgtgggcgcc gccggccatg ccggactcca gggcgatcca
     6121 gcccgcgtgc cggcccatga cctcgacgac catcacccgc tggtgggact cggcggtggt
     6181 cttgaggcgg tcgatcgcct cggtggccac gccgacggcg gtgtcgaaac cgaaggtgcg
     6241 gtccgtggag gagatgtcgt tgtcgatggt cttgggcacg cccacgaccg gcaggcccgc
     6301 gtccgacagc atccgggcgg cggtcagggt gccctcgccg ccgatcggga tgagcacgtc
     6361 gatgccgtac tcgcgggcga gctccttgga gctctcgcac gcctcgcgca gccgggcgcg
     6421 ctccaggcgg gaggagccga ggatggtgcc gccgcgggcg aggatgccgc tcacggcctc
     6481 caggtcgagc ttgcggtggc gtccgtcgag caggcccttg aagccgtctt cgaagccgat
     6541 gacctcgtca ccgtggccga cgaccgcgcg gtgcaccacc gagcggatga cagcgttcag
     6601 gccggggcag tcgccgcctg cggtgagaac tccgatgcgc atcgcgtgct gtgtctcctg
     6661 ctctggggtc ccccctaccg aaggtaaggg gatggtacat gtgagccggg ccgattgttt
     6721 cacggtccga tgggtaccga cgcaccccgg ggccgcgccg gccgccggcc tccgctcctg
     6781 cgagcgctgc cgcctgctga cgcgcgcctt atccaggggc gcaggtattg tcaagaggtc
     6841 aagcccacca taacgggtaa tttttaagcc gaagcacacg gcgaaaaaac accaagaaca
     6901 cggcgatgga gcaaggagag cacgcgtgac gcgcagcgtg tacgtgaccg ggaccgaccg
     6961 gggcgacggg cgccaggtca tcgagctggg agtcatggag ctcctcaccc gtcaggtgga
     7021 ccgggtgggc gtcttccgcc cgctcgtcca cggcgacggg ccggaccgcc tgttcgacct
     7081 gctgcggtcc cgctaccggc tgggccagtc cgcggagtcc ggccacggca tgagctacgc
     7141 cgaggcctcc gctctgcagg ccgagcgcgg cacggacgag ctggtctcgc ggctggtcga
     7201 gcgcttccac gcggtcgccc gcgactacga gtacgtgctg gtgctcggca ccgactacgt
     7261 cggctccacg ctccccgacg agctcgccct caacgcccgt ctcgccaacg agttcggcgc
     7321 ctcggtgatc acggtcgtcg gcggccagga ccagacggcc gagtccgtcc gggccgaggc
     7381 gcgcaacgcc taccgcgcct acgaggccct gggctgcgac gtcgtcgccc tggtcgtcaa
     7441 ccggatcgcc ccggccgacc gcgaggccgt cggcgagcgc ctctcggcga gcctgcccgt
     7501 cccctgctac gccctccccg aggaggccgg gctctccgcg ccgaccgtgg cgcagatcgt
     7561 ccgcaagctg gacgccgagg tgctgctcgg cgacgacgcg gggctcgccc gcgatgccaa
     7621 ggacttcgtc ttcggcggcg ccatgctgcc ggtcttcctg cccgccctga ccgagggctg
     7681 cctggtcgtg acccccggcg accgggtgga cctgatcgtc ggcgccctcg ccgcgcacag
     7741 cgccggcgcc ccgccgatag ccggcgtgct gctgaccctg gacgagaagc cgggtccgga
     7801 catcctggcg ctcgcggacc ggctggcccc gggcaccccg gtcgtctcgg tgccgggcgg
     7861 ttccttcccg accgcggccg agctgttcgc gatcgagggc aagctggacg cgttctcgcc
     7921 gcgcaaggcg gagacggccc tcggcctgtt cgagcggcac gtggacaccg cggagctgac
     7981 gaaccgcatc gccgtggccc gctccagccg cgtcaccccc atgatgttcg agcacgagct
     8041 gatcgagcgc tcccgctccg cccgccgccg catcgtgctg cccgagggca ccgaggagcg
     8101 cgtgctgcgc gccgcggacg tggtgctgcg ccgcgacatc tgcgacctca cgctgctcgg
     8161 cgaggagggc gcgatccgca agcgcgccgg cgagctgggc atcgatctgg cccaggcgca
     8221 gatcatcgac ccgcagacct ccccgctgcg cgagcgcttc gccgagatct acgccaaggc
     8281 ccgcgcccac aagggcatga ccgtcgagct cgcgcacgac gtggtcaccg acgtctccta
     8341 cttcggcacc ctgatggtcc aggagggcct ggccgacggc atggtctccg gcgcggtgca
     8401 ctccaccgcc gccaccatcc gcccggcctt cgagatcatc aagaccgcgc cgggcgcgca
     8461 gatcgtctcc tcggtcttct tcatgtgcct ggccgaccgg gtgctggtct acggcgactg
     8521 cgccgtcaac cccgacccca acgccgagca gctcgcggac atcgccatcc agtccgcggc
     8581 gaccgccgcc cagttcggcg tcgagccgcg gatcgcgatg ctgtcgtact ccaccggcac
     8641 ctccggctcc ggcgcggacg tcgacaaggt gcgcaaggcc accgagatcg tccgcgagcg
     8701 ccgcccggac ctcaaggtcg agggcccgat ccagtacgac gccgccgtgg acgccgcggt
     8761 cgccaagacc aagctgcccg gctcggaggt ggccggcaag gccaccgtgc tgatcttccc
     8821 cgacctcaac accggcaaca acacctacaa ggccgtccag cgctcggccg gcgccgtggc
     8881 cgtcggcccg gtcctgcagg gcctgcgcaa gcccgtcaac gacctctccc gcggcgcgct
     8941 ggtgcaggac atcgtgacca cggtcgcgat ctccgcgatc caggcccagg gcgaggccgg
     9001 gaacggaacc gcgccggacg cgcccgccgt ctgacgcccg tacggccgta cgggaccgcc
     9061 gcccggtccc nnnnnnnnnn nnnnggccgg cccaccgccc cccccacccc gacgtcgaga
     9121 cccccacaac ccggaaagcg ttcatgactg ccaaccccac ccgagtcctc gtcctcaact
     9181 ccggctcctc ctcggtcaag taccagctgc tcgacatggc cgacggcgcc cggctggccg
     9241 tgggcctggt cgagcgcatc ggcgaggaga cctcccgcct cgcgcacacc ccgctcgcca
     9301 cgggcggcgg caagcgcgag acggaggcgc cgatcgccga ccacgaggcc gcgctgcagg
     9361 cggtcgccgc ggagctggcc gcggacgggc tgggcctgga ctcgccggag ctcgccgcga
     9421 tcggccaccg ggtggtgcac ggcggccaga agttcaccgc gcccaccgtg atcaccgacg
     9481 aggtgctggc ggagatagag cgcctcgtgc cggtcgcccc gctgcacaac ccggccaaca
     9541 tcaccggcat ccgcaccgcg caggcgctcc gtcccgacct gccgcaggtc gccgtcttcg
     9601 acaccgcctt ccacaccacg atgccggagg ccgccgcccg ctacgccatc gacgtggcca
     9661 cggccgacgc ccacatggtg cgccgctacg gcttccacgg cacctcgcac gcctacgtct
     9721 cgcgcgagac cgccaagctg ctgggcaagg acccctccga ggtcaacgtc atcgtgctgc
     9781 acctgggcaa cggcgcctcg gcctccgcgg tccgcggcgg cgtgtgcgtc gacacctcca
     9841 tgggcctgac cccgctggag ggcctggtca tgggcacccg ttcgggtgac ctggacccgg
     9901 cggtcatcct tcacctgtcc cgcaacgccg ggatgtccac cgacgagatc gacgcgctgc
     9961 tcaacaagaa gagcggcctg atcggcctgt gcggcgacaa cgacatgcgc gagatccggc
    10021 gccgcatcga cgagggcgac gagcgggcgg cgctcgcctt cgacatctac gtgcaccggc
    10081 tgaagaagta catcggcgcc tactacgcgg tcctgggcaa ggtcgacgcc gtggccttca
    10141 ccgccggcgt cggcgagaac gcggcgcccg tgcgcgaggc ggccgtggcg ggcctggagc
    10201 agctgggcct ggccgtggac gccgagctca acgccgtccg ctccggcgag ccgcgcctga
    10261 tctcccccgg atccgcccgg gtcgcggtcg ccgtcgtgcc caccgacgag gagctggaga
    10321 tcgccaccca gacgtacgac ctggtcagcg cttgatcgtc ggccgt
//
