LOCUS       NZ_BMUT01000004        13124 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces hiroshimensis strain JCM 4586 sequence04, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUT01000004
VERSION     NZ_BMUT01000004.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces hiroshimensis
  ORGANISM  Streptomyces hiroshimensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 461946)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 90X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650335.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:32:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,830
            CDSs (total)                      :: 6,745
            Genes (coding)                    :: 6,620
            CDSs (with protein)               :: 6,620
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 25 of 125
            Pseudo Genes (incomplete)         :: 107 of 125
            Pseudo Genes (internal stop)      :: 4 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:07:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 54570
            Orig. end                         :: 67694
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUT01000004.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..13124
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="1"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..13124
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13124
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:8124](-)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..8124)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     gene            complement(600..1940)
                     /locus_tag="IE241_RS11165"
                     /old_locus_tag="GCM10010324_22950"
     CDS             complement(600..1940)
                     /EC_number="3.5.3.13"
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017241228.1"
                     /locus_tag="IE241_RS11165"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_22950"
                     /product="formimidoylglutamate deiminase"
                     /protein_id="WP_190021844.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 3.1e-48, bitscore:
                     159.3, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAQTYWAEHALLDTGVEPGVVIETGADGRIKAVRRGAAAPPPGA
                     VTLRGITLPGLANAHSHAFHRALRGATQAGEGTFWTWRDVMYRVADNLTPDNYFALARA
                     AYAEMALAGITSVGEFHYVHHLAGGARYDNPNAMGEALIAAAAEAGIRITLLDTCYLSS
                     GFGAEPNKHQRRFSDGTAERWAERVSALKGDDNTRIGAAIHSVRAVPAEELATVAAWAQ
                     EREAPLHLHLSEQTAENDACVEAHGFTPTALLSHHGVLGPRTTAVHATHLTPGDIRLLG
                     HSGTGVCMCPTTERDLADGIGPARALQEAGSPLSLGSDSHAVIDLLEEARAMEMDERLH
                     TRTRGHWTAVQLLRAASADGHAALGWDEAGRIEPGALADFTAVALDTVRTAGPPYVLAA
                     ETAVFAASAADVRDTVVGGRHIVRDGTHQLVAHVPAALAESIAAVRG"
     gene            complement(1937..3127)
                     /locus_tag="IE241_RS11170"
                     /old_locus_tag="GCM10010324_22960"
     CDS             complement(1937..3127)
                     /GO_function="GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amidines
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014177124.1"
                     /locus_tag="IE241_RS11170"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_22960"
                     /product="allantoate amidohydrolase"
                     /protein_id="WP_190021564.1"
                     /transl_table=11
                     /translation="MWADLGAVGRDKDTRGYHRFAWSGADADCRAWFRAQAESRGLAYE
                     LDRNGNQWAWLGDPAAGDAVVTGSHLDSVPNGGAFDGPLGVVSSFAAIDELRARGAQPS
                     RPLAVVNFGDEEGARFGLACVGSRLTAGQLSLDKAHALRDAEGVSLPQAMERAGYDPEA
                     IGPDPERLARIGAFVELHVEQGRALDLSDRAVGIASAIWPHGRWRFDFHGEANHAGTTR
                     IEDRRDPMLTYANTVLAARKKARLAGALATFGKVGVEPNGVNAIPSLVRGWLDSRAADQ
                     ETLDLVVAEIEKAAVERGERDGVDVRVTRESFTPVVEFEHALRDELARILGGDVPVLGT
                     GAGHDAGILSGAVPTAMLFVRNPTGVSHSPAETASEDDCLAGVTALADVLEGLACR"
     misc_feature    3087..3099
                     /note="TFBS match to MatP, Cell cycle regulator,
                     confidence: weak, score: 19.79"
                     /tool="antismash"
     misc_feature    complement(3120..3134)
                     /note="TFBS match to NrdR, Represses ribonucleotide
                     reductase encoding genes, confidence: medium, score: 24.02"
                     /tool="antismash"
     gene            complement(3280..4938)
                     /gene="hutU"
                     /locus_tag="IE241_RS11175"
                     /old_locus_tag="GCM10010324_22970"
     CDS             complement(3280..4938)
                     /EC_number="4.2.1.49"
                     /GO_function="GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hutU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014046405.1"
                     /locus_tag="IE241_RS11175"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_22970"
                     /product="urocanate hydratase"
                     /protein_id="WP_190021565.1"
                     /transl_table=11
                     /translation="MSGPRPVRAPRGSQLSTLGWQQEAALRMLQNNLDPEVAEHPDKLV
                     VYGGTGKAARDWRSFDAMVRTLQTLKQDETMLVQSGRPVGVMQTHEWAPRVLLANSNLV
                     GDWANWEEFRRLEHLGLTMYGQMTAGSWIYIGTQGILQGTYETFAAVAAKKFGGTLAGT
                     ITLTAGLGGMGGAQPLAVTMNDGVAICIDCDPRAIERRIEHRYLDVKADSLDHALQLAV
                     EARDARRPLSIGVLGNAAELLPQLLAMNAPIDIVTDQTSAHDPLAYLPVGISFDDMAAY
                     AAEKPADFTTRARESMARHVEAMVGFQDAGAEVFDYGNSIRGEAQLAGYTRAFDFPGFV
                     PAYIRPLFCEGKGPFRWAALSGDPADIHKTDKAMLELFPENESLARWIKMAGERVHFQG
                     LPARICWLGYGERDKAGERFNDMVADGTLAAPLAIGRDHLDCGSVASPYRETEAMLDGS
                     DAIADWPLLNAMVNVASGASWVSIHHGGGVGMGRSIHAGQVTVADGTALAGEKIRRVLT
                     NDPGMGVIRHVDAGYDIAESVASERGVRVPMREDS"
     gene            complement(5001..6524)
                     /locus_tag="IE241_RS11180"
                     /old_locus_tag="GCM10010324_22980"
     CDS             complement(5001..6524)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018843267.1"
                     /locus_tag="IE241_RS11180"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_22980"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_190021566.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 1.9e-25, bitscore:
                     84.3, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 1.2e-10, bitscore:
                     36.3, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAPSHIAAPLTAPPLVVVVGAGPAGARAALSAARCGARVALVDS
                     GERIGGQYHRQPAVAGKAVAGGPDASAAHPLELAVATQPRIAHLPRTTVWALEPLPEPG
                     PAPGRHLVHVQQGPADDRGRPVRTLAADALVLCTGAFDRALPFPGWELPGVVTAGAAQA
                     LARGQRTAVGGRVVVSGTGPFLLPVALALPEVGARVLGVYEAGNPARWLRSPAAALRDG
                     GPGRVAELGSYAAGLARHRVPYRTRHAVVAAHGRDRVEAVTVARLDAGWHPVAGSEQRI
                     GPVDAVCVGYGFTPQLELPLAAGCAVRGGHVAVDARQATTVPGVYAAGELTGVAGAPAA
                     AAEGELAGLAAVADLADLADLADLAERPWAAEAAAHLAPPSPARLAAAASRVRSGRRFA
                     RALARAHPVPGGWRGWLREDTLVCRCEEVSYGELRDAVAARGADGMRALKLTCRAGLGA
                     CQGRVCGRNTAELAAALLGAPVADPHAMDRRPLAHPVRLGDLAEPPPID"
     gene            complement(6521..6886)
                     /locus_tag="IE241_RS11185"
                     /old_locus_tag="GCM10010324_22990"
     CDS             complement(6521..6886)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019358493.1"
                     /locus_tag="IE241_RS11185"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_22990"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_190021567.1"
                     /sec_met_domain="Fer2_4 (E-value: 9.1e-24, bitscore: 78.0,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MALTVYVDGERHRALPGQTVAALLLATGRRSWRTTRLGGRPRGIF
                     CGIGVCYDCLVVVNGLPDVRACRRELCDGDRVETQRGARLPTEELPPEPRVPRTDGDGT
                     DQGARPGEPVRDADGVA"
     gene            complement(6877..8124)
                     /locus_tag="IE241_RS11190"
                     /old_locus_tag="GCM10010324_23000"
     CDS             complement(6877..8124)
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016642316.1"
                     /locus_tag="IE241_RS11190"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_23000"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_229899121.1"
                     /sec_met_domain="DAO (E-value: 6.4e-64, bitscore: 211.3,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRVLREDGEDRQVRDDGGGGADVVIVGSGVVGAACAEALTRRGAC
                     VLVLDRGPLAAGTTACGEGDLLVSNKAPGPGLTLAQASLSRWPRLLAALREELGPGRAE
                     CEYEVKGGLAVATGEPEGAALCDFAAAQRAAGVDARELAPGEAAAYEPHLTPGVRAAVH
                     YPQDAQLQPVLATTSLLAAVRARGGRIRTGVDVAGVETGRDGRAVGVRTSEGRIPCGAV
                     VNACGPWAGEFAAAAGAPLPVLPRRGTVLVTAPLPHGTVRHKVYDAGYADTARSADPGL
                     QVSAVVEATQAGTVLIGSSRQRCGFDGTLRAGVLRALARGAAQLFPVLGGVPVMRAYGG
                     FRPYTPDHLPVVGEDPRRPGLWHATGHEGAGIGLAAATGELLAELWAGEPPHLDPEPFR
                     VQRFAAARFGRENGWH"
     gene            complement(8259..8492)
                     /locus_tag="IE241_RS11195"
                     /old_locus_tag="GCM10010324_23010"
     CDS             complement(8259..8492)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE241_RS11195"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010324_23010"
                     /product="hypothetical protein"
                     /protein_id="WP_229899124.1"
                     /transl_table=11
                     /translation="MQTNENAAMPAVGTMVLDKRRDRVGEVMQTTPSRVFLRAFGGGRE
                     WIASLEDLEPVGVAEELRARVATVNAAQRWRP"
     gene            complement(8572..9747)
                     /locus_tag="IE241_RS11200"
                     /old_locus_tag="GCM10010324_23020"
     CDS             complement(8572..9747)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1148: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006605180.1"
                     /locus_tag="IE241_RS11200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_23020"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_190021847.1"
                     /transl_table=11
                     /translation="MGATIGQRLQDVRKRRGLSQRELAAASGVSLSLIRKLEQGERNDT
                     RLETARQLAVALGVTTSCLLAEPEARGEPPAGDRWEPVRQALAAGTLPPGPEEPPTVAG
                     VLGTLDAAVPLFSGDRYAELSTLLPPLIRDAEALAAAGPEGRAVRARVLQLTGWLLTQT
                     RQFAAAHSALDRSLDDAPDQLHGAATVSTQCWLLLRQGRLATARELAAEWADEVEPRMS
                     RASVAELSAWGWLLLRLSAASIRDNRPGEAEDSMRLAHAAAVAMGREFAPDRDFLRAFG
                     PVTVVLKRAENAMIADRPDVVLQLAERVPVGGMRPTSNNRNRHLLDVAEAHVRSRQYAE
                     AVDVLQGIRTAAPQWLPNQRYAHDILGRVISRRRTLTPGIRSLADAIGVPM"
     gene            10110..11456
                     /locus_tag="IE241_RS11205"
                     /old_locus_tag="GCM10010324_23030"
     CDS             10110..11456
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_Arg_deC_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_DAP_Arg_deC"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1264: diaminopimelate decarboxylase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018841897.1"
                     /locus_tag="IE241_RS11205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_23030"
                     /product="diaminopimelate decarboxylase"
                     /protein_id="WP_190021568.1"
                     /sec_met_domain="Orn_Arg_deC_N (E-value: 4.1e-37, bitscore:
                     122.5, seeds: 15, tool: rule-based-clusters)"
                     /sec_met_domain="Orn_DAP_Arg_deC (E-value: 9.5e-11,
                     bitscore: 36.5, seeds: 500, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSRDIPLGTVVQAAIRQGLLPAWSPVAGLLDVQGIRAATTSLRA
                     AFEEAGAARDTTLHAFAVKAAALVPVLRLLAGEGLGAEVASPGELALARAARVAPERIV
                     LDSPAKTTAELREALALGVAVNADSREELARLDGLLREAAGPPRSSLGVRVNPQVGGGT
                     IDALSTATDTSKFGVALRDEGARAWLVRAFAERPWLSRLHAHVGSQGCPLELMAAGVRA
                     TYELAEEINRAVGRRQVTTLDIGGGLPVNFASDDVTPTFRAYARLLADAAPGLFDGRYG
                     LVTEFGRSLLAKSGVVLARVEYTKTAGGRRIAVTHAGAQVAVRTVFAPASWPLRVAVFD
                     AQGRPKTGPPVDQDIAGPCCFAGDLVAVGRPLPLLEAGDHVALLDTGAYYFSNHYAYNS
                     LPRPGVYGYASAEEESDGVRFATVRAPQTLGELVAEGGGAYAAELSALR"
     gene            complement(11508..12110)
                     /locus_tag="IE241_RS11210"
                     /old_locus_tag="GCM10010324_23040"
     CDS             complement(11508..12110)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004951231.1"
                     /locus_tag="IE241_RS11210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_23040"
                     /product="hypothetical protein"
                     /protein_id="WP_190021569.1"
                     /transl_table=11
                     /translation="MGRGSYALARVAVVVRAPAGWLWWPGLLSAGIGVLVPGITGRRIG
                     VLAGAALFLIVAGAVFAVRRRRYAALARGASRAGRSVFLQDRAVTRRNWRRARRWWLLL
                     AFLAAVGSSFAIPAAAGLALAGAGAGLWAKALWLGGLERRSGSLLWVRTEWAQASPAAK
                     PVRGYLSTGLAAGDAAPGGGRRRAGRVPAGTAVSEAR"
ORIGIN
        1 cgggggagac gatgtgggcg ccgaggtagg tgacctcgtc ggtgtgcgcc gcggcgatgc
       61 gcagggcgcg ggcctcgtcc tcgacggtga ggccgtagcc ggacttggtc tccatgaccg
      121 tggtgccctg gcgcagggcc tcgcccacat agcggcggac gttggcgtcc agcacctcgt
      181 cggtcgcggc gcgcgtggcg gcgacggtgg tgcggatgcc gccggcgctg tagctgcggc
      241 cggacatgcg ggcgtggaac tcggcggtgc ggtcgcccgc gaagaccagg tgggagtggg
      301 agtcgacgaa gccggggagg gcggcccggc cctcggcgtc gacgcgattg tcagtggccg
      361 gtgctttgct ggacgtgccg acccaggcga cgcggtcgcc gtcgatgacg actgccgcgt
      421 cctggatcgt gccgaggggg ccttcgccga gggaggggtc gttcgtgacg agactgctga
      481 tgtgggtgat gaccgtcgtc gccatgggcg tgcggcactc cttaggtcgg ttcggtggtt
      541 cggcctcgcg cgaggccgaa ccgcacgggt cggcctcgta cgggccggcc tcgtacggtt
      601 cagcctcgta cggccgcgat cgactccgcc agtgccgccg gcacgtgggc gacgagctgg
      661 tgcgtcccgt cgcgcacgat gtgccggccg cccaccaccg tgtcccggac gtccgcggcg
      721 ctcgccgcga agacggccgt ctcggcggcg aggacgtagg gcgggcccgc ggtgcgcacg
      781 gtgtcgaggg cgacggccgt gaagtccgcg agcgcgcccg gctcgatgcg gcccgcctcg
      841 tcccagccga gggccgcgtg gccgtcggcg gaggcggcgc gcagcagctg gacggcggtc
      901 cagtggccgc gggtgcgggt gtgcagccgc tcgtccatct ccatggcgcg cgcctcttcg
      961 agcaggtcga tgacggcgtg gctgtcgctg ccgagggaga gcggggagcc cgcctcctgc
     1021 agcgcccggg ccgggccgat gccgtcggcc aggtcccgct cggtggtcgg gcacatgcac
     1081 acgccggtgc ccgagtggcc gaggaggcgg atgtcgccgg gggtgaggtg cgtggcgtgg
     1141 acggcggtgg tgcgcgggcc gagcacgccg tggtgggaga ggagcgcggt gggggtgaag
     1201 ccgtgcgcct cgacgcaggc gtcgttctcg gcggtctgct cggacaggtg caggtgcagc
     1261 ggggcttcgc gctcctgcgc ccacgcggcc acggtcgcca gctcctcggc gggcacggcc
     1321 cgcaccgagt ggatcgccgc gccgatgcgg gtgttgtcgt cgcccttgag ggcggagacg
     1381 cgctcggccc agcgctcggc ggtgccgtcg ctgaagcgcc gctggtgctt gttcggctcg
     1441 gcgccgaagc cggaggagag atagcaggtg tcgaggagcg tgatgcggat gccggcctcg
     1501 gcggcggccg cgatcagcgc ctcgcccatg gcgttggggt tgtcgtagcg tgcgccgccg
     1561 gcgaggtggt gcacgtagtg gaactcgccg acggaggtga tgcccgcgag cgccatctcg
     1621 gcgtaggccg cccgggccag cgcgaagtag ttgtccgggg tgaggttgtc cgcgacgcgg
     1681 tacatgacgt cgcgccaggt ccagaaggtg ccctcgcccg cctgggtcgc cccgcgcagg
     1741 gcccggtgga aggcgtggct gtgcgcgttc gcgaggccgg gcagggtgat gccccgcagg
     1801 gtcaccgcgc ccgggggcgg ggccgccgcg ccccggcgga cggccttgat gcggccgtcc
     1861 gccccggtct cgatgaccac gcccggctcg acgccggtgt cgagaagggc gtgctccgcc
     1921 caataggtct gtgccgtcag cgacacgcca ggccctccag tacgtcggcg agtgcggtca
     1981 ccccggccag gcagtcgtcc tcggaggccg tttcggcggg cgagtgggag acgcctgtgg
     2041 ggttgcgtac gaacagcatg gcggtgggga cggctccgga gaggattccg gcgtcgtgtc
     2101 ccgcgcccgt cccgaggacg gggacgtccc cgcccaggat ccgggccagc tcgtcgcgca
     2161 gggcgtgctc gaattccacg accggcgtga aggactcccg ggtgacccgc acgtccacgc
     2221 cgtcgcgctc gccgcgctcc acggccgcct tctcgatctc cgcgaccacg aggtcgaggg
     2281 tctcctggtc ggcggcccgc gagtccagcc agccgcgtac gagggagggg atggcgttga
     2341 cgccgttcgg ctcgacgccg accttcccga aggtggcgag ggctccggcg agccgggcct
     2401 tcttgcgggc ggcgaggacg gtgttggcgt aggtcagcat cgggtcccgg cggtcctcga
     2461 tgcgggtggt gccggcgtgg ttggcctcgc cgtggaagtc gaaccgccag cggccgtgcg
     2521 gccagatggc ggaggcgatg ccgacggcgc ggtccgacag gtccagggcg cggccctgct
     2581 cgacgtggag ctcgacgaag gcgccgatcc gggcgagccg ctcggggtcc gggccgatgg
     2641 cctccgggtc gtagccggcg cgctccatgg cctgcggcag ggagacgccc tcggcgtccc
     2701 ggagcgcgtg tgccttgtcg agggacagct gacctgcggt caggcgcgag cccacgcagg
     2761 ccaggccgaa gcgggcgccc tcctcgtcgc cgaagttgac gacggcgagg gggcgggacg
     2821 gctgcgcccc gcgggcgcgc agctcgtcga tcgcggcgaa ggaggagacg acgccgaggg
     2881 ggccgtcgaa ggcgccgccg ttcggtacgg agtccaggtg ggaccccgtg acgacggcgt
     2941 ccccggcggc ggggtcgccg agccacgccc actggttgcc gttgcggtcg agctcgtagg
     3001 ccagcccccg gctctccgcc tgggcgcgga accaggcgcg gcagtcggcg tcggcgccgc
     3061 tccaggcgaa gcggtggtag ccgcgggtgt ctttgtcacg gccgacggcg cccaggtcgg
     3121 cccacatctc gtggaacgag gagggcccaa ccgggcccgc actcggcgac ggcgcgtccg
     3181 ttccgccaga ggtttccgct acggctgcgg gccggtggtc gcgttgtgcc cacccgttcc
     3241 gccctgcgga acgattgccc acaacggggt cgggtccggt catgagtctt cccgcatcgg
     3301 gacgcggacg ccccgctcgg aggcgaccga ctccgcgatg tcgtacccgg cgtcgacgtg
     3361 ccggatgacg cccatccccg ggtcgttcgt caggacgcgg cggatcttct cgcccgcgag
     3421 cgccgtgccg tcggcgaccg tgacctggcc cgcgtggatg gagcggccca tgccgacgcc
     3481 gccgccgtgg tggatggaga cccacgaggc gcccgaggcg acgttgacca tggcgttgag
     3541 cagcggccag tccgcgatcg cgtcggagcc gtccagcatg gcctcggtct cgcggtacgg
     3601 cgaggccacc gatccgcagt cgaggtggtc gcggccgatg gccagcggcg cggcgagcgt
     3661 gccgtcggcg accatgtcgt tgaagcgctc gccggccttg tcgcgctcgc cgtagccgag
     3721 ccagcagatg cgggcgggca ggccctggaa gtggacccgc tcgccggcca tcttgatcca
     3781 gcgggcgagc gactcgttct cggggaagag ttcgagcatc gccttgtcgg tcttgtggat
     3841 gtcggccggg tcgccggaga gggccgccca gcggaagggg cccttgccct cgcagaagag
     3901 ggggcggatg taggcgggga cgaagccggg gaagtcgaag gcgcgggtgt agcccgcgag
     3961 ctgggcctcg ccgcggatgg agttgccgta gtcgaagacc tcggcgccgg cgtcctggaa
     4021 gccgaccatg gcctcgacgt gacgcgccat cgactcgcgc gcccgggtcg tgaagtccgc
     4081 gggcttctcg gccgcgtagg cggccatgtc gtcgaaggag atgccgaccg gcaggtaggc
     4141 gagcgggtcg tgggcgctgg tctggtcggt cacgatgtcg atcggggcgt tcatcgcgag
     4201 cagctgcggg aggagctccg cggcgttgcc gaggacgccg atggacagcg ggcggcgggc
     4261 gtcgcgcgcc tcgacggcca gctggagggc gtggtcgagc gagtcggcct tcacgtccag
     4321 gtagcggtgc tcgatgcggc gctcgatggc gcgcggatcg cagtcgatac agatggcgac
     4381 gccgtcgttc atggtgacgg cgagcggctg ggcgccgccc atgccgccga ggccggcggt
     4441 cagggtgatc gtgccggcga gggtgccgcc gaactttttg gcggcgacgg cggcgaaggt
     4501 ctcgtacgtg ccctgcagga tgccctgcgt gccgatgtag atccacgacc cggcggtcat
     4561 ctggccgtac atggtgaggc cgaggtgctc caggcggcgg aactcctccc agttggccca
     4621 gtcgccgacg aggttggagt tggcgaggag gacgcgcggc gcccactcgt gggtctgcat
     4681 gacgccgacc gggcggccgg actggacgag catggtctcg tcctgcttga gcgtctgcag
     4741 cgtgcggacc atggcgtcga acgagcgcca gtcgcgggcg gccttgcccg tgccgccgta
     4801 gacgacgagc ttgtcggggt gctcggccac ctcggggtcg aggttgttct gcagcatgcg
     4861 cagagcggct tcctgctgcc accccagcgt gctcagctgg ctgcctcgcg gcgctcgtac
     4921 gggtcgcggt ccggacatgg tgccctgcct ccctggcggc tctgaataga acactgcaca
     4981 tcttgggtgg ctgaatagct ctagtcaatg ggtggcggct ccgcgaggtc cccgagccgc
     5041 accggatggg ccagcggccg ccggtccatg gcgtgcggat cggcgacggg ggcaccgagg
     5101 agcgccgcgg cgagctcggc cgtgttgcgc ccgcagacgc gcccctggca ggcgcccagc
     5161 ccggcgcggc acgtgagctt gagcgcccgc atcccgtccg cgccgcgcgc cgcgacggcg
     5221 tcccgcagct ccccgtaaga cacctcctcg caccggcaca cgagcgtgtc ctcgcgcagc
     5281 cagccgcgcc agccgcccgg gacgggatgc gcccgggcca gcgcccgcgc gaaccgccgc
     5341 ccggaccgca cccgcgaggc ggcggccgcg aggcgggcgg gggacggcgg cgcgaggtgc
     5401 gccgcggcct ccgcggccca cgggcgctcc gccaggtcgg ccaggtccgc caggtccgcc
     5461 aggtccgcca ccgctgcgag ccccgccagc tcgccctccg ccgccgcggc cggggcgccc
     5521 gcgacgcccg tgagctcgcc cgccgcgtac acgcccggga cggtcgtcgc ctggcgcgcg
     5581 tcgacggcca cgtgcccgcc gcggaccgcg cagcccgcgg ccagggggag ctccagctgc
     5641 ggggtgaagc cgtagccgac gcacacggcg tccacgggcc cgatgcgctg ctcactgccc
     5701 gccaccgggt gccagcccgc atccaggcgg gcgacggtga cggcctcgac gcggtcccgg
     5761 ccgtgcgcgg cgacgaccgc gtgccgcgtg cggtacggga cgcggtggcg ggccagcccc
     5821 gcggcgtacg agcccagctc ggcgacccgg ccggggccgc cgtcgcgcag ggccgcggcg
     5881 gggctgcgca gccagcgcgc cgggttcccc gcctcgtaca cgcccaggac ccgcgccccc
     5941 acctccggca gggccagcgc gaccggcagc aggaacggcc cggtgcccga caccacgacc
     6001 cggccgccga cggccgtccg ctgccccctg gcgagcgcct gcgccgcgcc cgccgtgacc
     6061 acgcccggca gctcccagcc ggggaacggc agcgcgcggt cgaaggcgcc ggtgcacagg
     6121 acgagcgcgt cggccgcgag ggtgcgcacg gggcgtccgc ggtcgtcggc ggggccctgc
     6181 tggacgtgga cgagatggcg gccgggcgca ggccccggct ccgggagcgg ctccagggcc
     6241 cagacggtgg tgcggggcag gtgggcgatg cggggctggg tggcgacggc cagttcgagc
     6301 gggtgcgcgg cggaggcgtc gggcccgccc gcgacggcct tgcccgcgac ggccggctgc
     6361 cggtggtact ggccgccgat gcgctccccg ctgtcgacga gcgcgacgcg tgcgccgcac
     6421 cgggcggcgc tcagcgcggc ccgcgcgccg gcgggtcctg cgccgacgac gaccacgagg
     6481 ggcggggcgg tgaggggcgc ggctatgtgg gacggggcgc tcatgcgacc ccgtccgcgt
     6541 cgcgtacggg ctcgccgggc ctggcgccct ggtccgtacc gtcgccgtcc gtacgaggta
     6601 cacgaggttc cggaggcagc tcctccgtgg gcagccgggc gccgcgctgg gtctccaccc
     6661 ggtccccgtc gcacagttcg cgccggcagg cccggacgtc ggggaggccg ttgacgacga
     6721 ccaggcagtc gtagcagacg ccgatgccgc agaagatgcc gcgcgggcgg ccgccgaggc
     6781 gggtggtgcg ccaggaccgg cggcccgtcg cgaggagcag ggccgccacc gtctggccgg
     6841 gcagggcgcg gtgccgctcg ccgtcgacgt acaccgtcag tgccatccgt tctcccttcc
     6901 gaagcgtgcg gctgcgaagc gctggacccg gaacggctcc ggatcgaggt gcggcggctc
     6961 gcccgcccag agttcggcga gcagctcgcc ggtggcggcg gccaggccga tgccggcgcc
     7021 ctcgtggccc gtcgcgtgcc acaggccggg gcggcgcggg tcctcgccga cgaccgggag
     7081 gtggtcgggg gtgtacgggc ggaagccgcc gtacgcgcgc atgacgggga cgccgccgag
     7141 gacggggaac agctgcgcgg ccccgcgcgc cagcgcgcgc agcacgccgg cgcgcagcgt
     7201 cccgtcgaag ccgcagcgct gccggctgga gccgatgagg accgtgcccg cctgggtcgc
     7261 ctcgaccacg gccgacacct gcagccccgg gtccgcgctg cgggcggtgt ccgcgtaccc
     7321 ggcgtcgtag accttgtgcc gcaccgtgcc gtgcggcagc ggtgccgtga cgaggaccgt
     7381 gccgcggcgc ggcaggacgg gcaggggtgc cccggcggcc gcggcgaact ccccggccca
     7441 ggggccgcag gcgttgacca ccgccccgca cggaatgcgg ccctccgacg tccggacgcc
     7501 cacggcccgc ccgtcccggc cggtctccac gcccgccacg tccacgccgg tacggatccg
     7561 gccgccccgc gcgcggaccg ccgccagcag cgacgtcgtc gccaggacgg gctgcagctg
     7621 ggcgtcctgc gggtagtgca cggcggcgcg cacgccgggc gtgaggtgcg gctcgtacgc
     7681 ggcggcctcg cccggcgcga gctcgcgggc gtccacgccc gccgcgcgct gggcggcggc
     7741 gaagtcgcac agggcggcgc cctccggctc gccggtggcg acggcgagac cgcccttcac
     7801 ctcgtactcg cactccgccc ggcccgggcc gagctcttcg cgcagcgcgg cgagcagccg
     7861 gggccagcgg ctcagcgagg cctgcgcgag ggtgaggccg gggccggggg ccttgttcga
     7921 gacgaggagg tcgccctcgc cgcaggcggt ggtaccggcc gcgagcggac cccggtcgag
     7981 gacgagtacg caggccccgc ggcgggtcag cgcctcggca caggccgcgc cgacgactcc
     8041 gctcccgacg atgacgacgt cggcgccgcc gccaccgtcg tcccggactt gccggtcttc
     8101 cccgtcttcc cgcagaacgc gcacgccagt agcaccgccg ttcccccaga ggccgccgcg
     8161 acgctattcg ccccgcgccg ggcgggtcaa cacccgcggc gcggttcccc ggtccacgtt
     8221 tgtgcagggc cgccgcgcgg cgggagcggc accactgttc acggccgcca ccgctgcgcg
     8281 gcattgaccg tggccaccct ggcccgcagc tcctcggcga cgccgacggg ctccagatcc
     8341 tcaagggagg cgatccactc ccgcccgccc ccgaaggccc ggaggaacac acgcgacggc
     8401 gtcgtctgca tcacctcacc gacgcggtcg cgccgcttgt ccagcaccat cgttccgacc
     8461 gcgggcatcg ctgcgttctc gttcgtctgc atgacacatg acgctaagga ccaggcgttt
     8521 tgccgggcaa tggcactctg tggcggtggt cgacggaagt gccaccgcgc gtcacatggg
     8581 gacgccgatg gcgtcggcca gggaccgtat gccgggggtg agggttctcc tccggctgat
     8641 cacgcgaccg aggatgtcgt gtgcgtagcg ctggttgggc agccactggg gtgccgcggt
     8701 ccggatgccc tggaggacat cgaccgcctc cgcgtactgc cgtgagcgca cgtgggcttc
     8761 ggctacgtcc aggaggtggc ggttgcggtt gttggacgtg gggcgcatgc cgcctaccgg
     8821 cactcgttcg gcgagttgca ggacgacgtc cggccggtcg gcgatcatcg cgttctccgc
     8881 gcgcttgagg acgacggtca cggggccgaa ggcgcgcagg aagtccctgt ccggggcgaa
     8941 ctccctgccc atggccacgg ctgcggcgtg cgccagccgc atggagtcct cggcttcgcc
     9001 gggccggttg tccctgatgg acgcggcgga gaggcgcagc agcagccacc cccatgcgct
     9061 cagctcggcg acggaggcgc gggacatgcg cggctccacc tcgtcggccc actcggccgc
     9121 cagttcacgg gctgtggcca gccggccctg ccggagcagc agccagcact gggtgctcac
     9181 ggtggcggcc ccatggagct ggtccggcgc gtcgtccagg gaccggtcga gggcggagtg
     9241 cgctgcggcg aactgccgtg tctgggtcag cagccacccg gtcagctgca ggacccgggc
     9301 gcgcaccgcc ctcccctcgg gcccggctgc ggccagcgcc tcggcgtcgc ggatcagcgg
     9361 cggcagcaac gtgctcagct cggcgtaccg gtcaccggag aagagcggca cggccgcgtc
     9421 gagggtgccg aggactccgg cgaccgtggg cggctcctcg ggtccgggcg gcagtgtccc
     9481 tgcagcgagg gcctgccgca cgggctccca ccggtccccg gccgggggtt ctcccctggc
     9541 ttccggttcc gcgaggaggc agctggtcgt gacgcccaac gcgacggcga gctgccgggc
     9601 cgtctccaga cgggtgtcgt tccgctctcc ctgttcgagt ttgcggatca gcgagagcga
     9661 gacaccggac gccgcggcaa gttcccgttg gctgaggccc cggcgcttgc gcacgtcttg
     9721 gagccgttga ccgatagtgg cacccatggg catggggcga gcgtacgccc ctatgaatac
     9781 gggaaggttg acgaatggcc cggaccgtcg gcggggctct cgccgtctcc gaggagccga
     9841 accgatggcg ggtccgtggt gcggcccggc cgcgtgtgcg aggaacgggc agcccaggta
     9901 catgccaccg ccgaggcggc gcccatcgac cggcaccgat gaaccggtcg aaggcgtccc
     9961 catgcccggg ccgacccggt tcgcaccccg ttccggcccg cggcggctcc gcgcacgcgc
    10021 gggcgtgccg tcgcgcatgg agctcatcgt gtgcggaaac cgcccgcagc gcatgccatt
    10081 ttcgcggccc ggtgtggtgg agtggctcca tgtcctcccg tgacatcccc ctcggcaccg
    10141 tggtgcaggc cgcgatccgg caagggctgc tgcccgcctg gtcccccgtc gccgggctgc
    10201 tcgacgtgca ggggatccgc gcggccacca cgtcgctgcg ggcggccttc gaagaggccg
    10261 gggcggcgcg cgatacgacc ctgcacgcgt tcgccgtgaa ggccgccgcc ctcgtgcccg
    10321 tgctgcggct gctggccggg gaagggctgg gcgccgaggt ggcgagcccc ggtgagctgg
    10381 cgctggcccg ggcggcgcgg gtggccccgg agcggatcgt gctggactct cccgcgaaga
    10441 ccacggcgga gctccgtgag gccctcgcgc tgggggtggc ggtcaacgcg gacagccgcg
    10501 aggagctcgc ccggctggac gggctgctgc gggaggccgc cggcccgccc cgctcctcgc
    10561 tcggcgtgcg tgtcaacccc caggtgggcg gcggcaccat cgacgcgctg agcacggcga
    10621 cggacacgtc gaagttcggg gtggcgctgc gcgacgaagg ggcgcgcgcc tggctcgtcc
    10681 gcgccttcgc cgagcgcccc tggctgtccc ggctgcacgc ccacgtgggc tcccagggct
    10741 gccccctgga gctgatggcg gcgggggtgc gggccacgta cgagctggcg gaggagatca
    10801 accgggccgt ggggcgccgg caggtcacca ccctggacat cggcggcggg ctgcccgtga
    10861 acttcgcgtc cgacgacgtg accccgacct tccgcgcgta cgcccgtctg ctggcggacg
    10921 ccgccccggg cctcttcgac gggcgctacg ggctggtgac ggagttcggc cgctccctgc
    10981 tggccaagag cggggtggtg ctggcccgcg tcgagtacac caagaccgcg ggcgggcggc
    11041 ggatcgccgt gacgcacgcc ggcgcccagg tggccgtgcg cacggtgttc gcgcccgcgt
    11101 cctggccgct gcgggtggcg gtcttcgacg cacaggggcg cccgaagacc gggccgccgg
    11161 tcgaccagga catcgcgggg ccgtgctgct tcgcgggcga cctcgtggcg gtgggcaggc
    11221 cgctgcccct gctggaggcg ggcgaccacg tggcgctgct cgacaccggc gcgtactact
    11281 tctcgaacca ctacgcgtac aactctctgc cccgccccgg ggtttacggc tacgcgtccg
    11341 cagaagagga gagtgacggc gtgcggttcg cgaccgtacg ggccccgcag accctcggcg
    11401 agctcgtcgc cgagggcggg ggcgcctacg cggcggagct cagcgcgctt cgctgaccgg
    11461 cccccggacc tcggaccccg gaccccggcg ccctcgccgg gcaggcgtca gcgcgcttcg
    11521 ctgaccgccg tcccggccgg cacccgtccg gcgcggcgcc ggccgccacc gggagcggca
    11581 tcgcccgcgg cgagccccgt ggacagatag ccgcgcacgg gcttggcggc gggggacgcc
    11641 tgcgcccact ccgtgcggac ccacagcagg gaaccgctcc ggcgctccag gccgcccagc
    11701 cacagggcct tcgcccacag gcccgcaccc gctccggcga gcgccaggcc cgccgcggcc
    11761 gggatcgcga aggaggagcc cacggcggcg aggaaggcca ggagcagcca ccagcggcgg
    11821 gcgcgccgcc agttccgccg ggtcacggcg cggtcctgga ggaagaccga ccggcccgcc
    11881 cgtgacgcac cgcgcgccag ggccgcgtac cgcctgcggc gcaccgcgaa gacggcgccc
    11941 gcgacgatca ggaacagcgc cgccccggcg agcacgccga tccgccgccc ggtgatcccg
    12001 ggcaccagga cgccgatgcc cgcactcagc aggcccggcc accacagcca gccggccggc
    12061 gctctgacga ccaccgccac ccgcgccagc gcatacgaac cgcgccccac gtcgaaaccc
    12121 gcctttccct ctgaaatccc ctcgatcccg actcgtcacg cggcaggcta gccaacgaat
    12181 ctgagtgcga gctgagaatc cgcgggatgt acctcacagg ggtcataccc cggtatcacc
    12241 ccggacttgg ctcctccgta tgacgtgtgc gcagtcgtgt ggtccctaag gtcgtccctg
    12301 ccggggaggg acgagcggga ggagacgacg atggcggtcc aggaacgagg gggcgagcag
    12361 ccggaccgcg ggcggcggga cgacggtctc cggcggcggg ctgccgtcgc ggcgggggcg
    12421 ctggccctga ccgggctgct gagcgcgctc gtgcccgcgc cggaggccca tgcggcgcag
    12481 gcccctgccg cagccggagc cgaggcctca gccggagctg gagccggggc cggggccggg
    12541 gccggggccg ccgaccagag cacccggacc gcgctgcccg cgcccaccgg cccccacgcc
    12601 gtcggcacga ccacccgcca cctggtggac accggccgca aggacccctg gaagccggag
    12661 cggggcgacc gccaggtcgc gatcagcctc ttctacccgg cggaccggga cggggccacc
    12721 ggcgggaagg gtcccgcgcc ccacatggac ccctcctccg ccgcccactt cggctccgcc
    12781 cggggcgcgg gcagcttcaa ctaccgcgtg cacccggacg ccgccgactg gacctccacg
    12841 cggacccacg cgcgcgtgga cgcccccgtg gcgcgcggca gcagggcgct gccggtcgtg
    12901 ctgtactccg ccggcctggg cgacccgcgc acctggaaca ccggcctggt ggaggagctg
    12961 gcctcccgcg gctacgtcgt cgtcaccgtc gaccacacct acgactcctc cgaagtgcgc
    13021 ctggccgacg gcagcctcgc ggagtcggcg ctgccggcgc tcgccggcgc gtccccggcc
    13081 gaggtcgacg ccgcgctccg cagggcggtg caggcccggg tgga
//
