LOCUS       NZ_BMUT01000003        20739 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces hiroshimensis strain JCM 4586 sequence03, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUT01000003
VERSION     NZ_BMUT01000003.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces hiroshimensis
  ORGANISM  Streptomyces hiroshimensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 582173)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 90X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650335.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:32:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,830
            CDSs (total)                      :: 6,745
            Genes (coding)                    :: 6,620
            CDSs (with protein)               :: 6,620
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 25 of 125
            Pseudo Genes (incomplete)         :: 107 of 125
            Pseudo Genes (internal stop)      :: 4 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:07:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 30786
            Orig. end                         :: 51525
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUT01000003.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..20739
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="CDPS"
                     /region_number="1"
                     /rules="CDPS"
                     /tool="antismash"
     cand_cluster    1..20739
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="CDPS"
                     /kind="single"
                     /product="CDPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..20739
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="False"
                     /core_location="[<10000:10739](+)"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      <10001..10739
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="CDPS"
                     /neighbourhood="10000"
                     /product="CDPS"
                     /protocluster_number="1"
     gene            13..396
                     /locus_tag="IE241_RS08920"
                     /old_locus_tag="GCM10010324_18270"
     CDS             13..396
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016644874.1"
                     /locus_tag="IE241_RS08920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_18270"
                     /product="VOC family protein"
                     /protein_id="WP_190021130.1"
                     /transl_table=11
                     /translation="MKAPAIAPCLGVKDTQASVDFYEKIGFTALPAGDDPNDDIRSLLF
                     GEDFALMVYRDAHLRDWLPVLKDRTPGAFGMFYLAVDDFAGYVAKIRPLVNATKEATHN
                     GQNLFYFTDPDGYVIGVVQKQEW"
     gene            402..815
                     /locus_tag="IE241_RS08925"
                     /old_locus_tag="GCM10010324_18280"
     CDS             402..815
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003987126.1"
                     /locus_tag="IE241_RS08925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_18280"
                     /product="VOC family protein"
                     /protein_id="WP_190021131.1"
                     /transl_table=11
                     /translation="MTTTKASTPVAKVASYAFWARDGKELARFYAAALGFQLVQEYPDE
                     QGVPTAFLVSNADGSVLYTFYTARDYRAPAWPQDELPFHLDLSFDDVEAGEKRLLELGA
                     TKPDHQPGGGQWTVLLDPSGQPFCISAAANWQY"
     gene            <1480..2343
                     /locus_tag="IE241_RS08930"
                     /old_locus_tag="GCM10010324_18300"
                     /pseudo=""
     CDS             <1480..2343
                     /GO_function="GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA]"
                     /GO_process="GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF017984.5"
                     /locus_tag="IE241_RS08930"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_18300"
                     /product="DNA glycosylase AlkZ-like family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="LDHLRTTLGWTPARYAAVEKATLDALVARGRASAAELAADIPDLR
                     EGYTLNAGKSYETRQRVTAPILGTLAAEGRIRRSRPAGSWTSAQFRWEPAEPLPAIPAA
                     EAQTMLARHYLAAFGPVTAADLKWWTGWTLTDTRKAIAATGAEAVELDEGTGYLLAEDN
                     QHLAEIDHEPWVALLPGLDPTSMGWRHRDFYLDPHHTAALFDRNGNIGPTVWADGRIIG
                     AWAQHPDGHINHHLLTDPGRHTRALINNAIQRLAIFLDGTRVTPCYRTPLERQLATTAA
                     PATRHG"
     gene            2787..4271
                     /locus_tag="IE241_RS08935"
                     /old_locus_tag="GCM10010324_18310"
     CDS             2787..4271
                     /EC_number="2.4.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014150522.1"
                     /locus_tag="IE241_RS08935"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_18310"
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="WP_190021132.1"
                     /transl_table=11
                     /translation="MSTRRTVRLSSPSPDRAATWAPVIVGLIGLGIGLRGITRPSAWTD
                     EIVTLDVARRSWPQLVELLGRVDAVHGLHYVLMYLVGQVAGVSEFTMRLPSAVAVAVAA
                     AGLGWLGRLLGGPRLGLYAGLLLAVLPTVSRYAQEARSFAFVMAVAVLATGALVKALAG
                     RARWAAGYAVLIALLGWFNVMGLLLVAAHAVTIALRRPGRRVVMRLLPAQAAGITAVIP
                     LLLLGFSQRSAVGEVPQVTAGTPYSYFTWLLTPGQDTLPAALKLLLTLTSLAAISLLVA
                     RRHTAPPLVFAIGVPWLAVPPLLLLALSLGQPLFAYRYLLFCLPALALLLAAAAALLPP
                     RRQLLLALLPALPITMSHLAIRQQDSRPWDTSTVITTLRSRAVPGDAILFSGARCDLIA
                     TTHPEIFARLPDLGTARTAAERGTLNNLPADSTLLNQRLARAPRVWHITCRHLSPGAYR
                     TGDRTTVAQEYALTRTGHTPAYHRSTRGLDITLYQR"
     gene            complement(<4554..4718)
                     /locus_tag="IE241_RS34090"
                     /pseudo=""
     CDS             complement(<4554..4718)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024920.5"
                     /locus_tag="IE241_RS34090"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="sterol carrier protein domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MASLDLVLDVRDDLCPWNTAPHRLRADGADVSCERTNAPADLRLT
                     AAELGAAFLG"
     misc_feature    complement(4677..4679)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(<4801..4941)
                     /locus_tag="IE241_RS34095"
                     /pseudo=""
     CDS             complement(<4801..4941)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF037610.5"
                     /locus_tag="IE241_RS34095"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPPGGPSGGTGVVRVVELAALSRQAYAALWFFLARLDLVTWIDYE
                     GA"
     gene            complement(5023..5457)
                     /locus_tag="IE241_RS08945"
                     /old_locus_tag="GCM10010324_18320"
     CDS             complement(5023..5457)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018888930.1"
                     /locus_tag="IE241_RS08945"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_18320"
                     /product="VOC family protein"
                     /protein_id="WP_190021133.1"
                     /transl_table=11
                     /translation="MASIKKFQVTFDCAEPERVARFWCEVLGYVVPPPPEGFATWDDFN
                     RSLPPESQGSAFACMDPSGVGPRMYFQRVPEGKVVKNRVHLDVRVGTGLVGEERLATLE
                     AESARLIALGAVRVRLLPADDENESCIVMQDIEGNEFCLD"
     gene            5799..6323
                     /locus_tag="IE241_RS08950"
                     /old_locus_tag="GCM10010324_18330"
     CDS             5799..6323
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006380588.1"
                     /locus_tag="IE241_RS08950"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_18330"
                     /product="DUF6817 domain-containing protein"
                     /protein_id="WP_190021134.1"
                     /transl_table=11
                     /translation="MAMLQAAGAAEIEHPGGTLLAHLQRVSALLSAWGARPALVSAGLC
                     HAFYGTDGFPVALLDLGRRAELAEAIGTEAEALVYFYASCDRKSSYRGLTDDTGLFTDR
                     FTGTRTHPGLEARRDFAELTAANELDIAAISPQMRARHGADLLALFTRWRPLLSEPAWT
                     HCRSVLGQADH"
     misc_feature    5808..5810
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(6764..7687)
                     /locus_tag="IE241_RS08955"
                     /old_locus_tag="GCM10010324_18340"
     CDS             complement(6764..7687)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003984015.1"
                     /locus_tag="IE241_RS08955"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_18340"
                     /product="lysozyme"
                     /protein_id="WP_190021135.1"
                     /transl_table=11
                     /translation="MSEHHSGPGRPHPPTAAAVLLSLLTALTLLVTLPGTAAPAAGSST
                     GNSAGSSSSPIHPEQDWMGSTVRAHEGERSPATKGSSLPDPSLPDVSPEGPSGSAGVKG
                     VDVSSHNGNVSWSKLWNSGVRFAYSKATEGTSYTNPHFAQQYNGPYDAGMIRGAYHFAL
                     PGNSGGAAQADYFVHHGGGWTDDGWTLPGVLDMEYNPYGSPCYGLSTGRMIDWIDDFLD
                     AYWDHTGRDAVIYTSTAWWKKCTGNYGGFGKYNPLWVPRYGSSSGELPNGWGFYTFWQH
                     TDTGPTVGDHDRFNGVYNRLKALAYG"
     gene            complement(7734..7931)
                     /locus_tag="IE241_RS08960"
                     /old_locus_tag="GCM10010324_18350"
     CDS             complement(7734..7931)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE241_RS08960"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010324_18350"
                     /product="hypothetical protein"
                     /protein_id="WP_190021136.1"
                     /transl_table=11
                     /translation="MAGSIWSEVPTSGLVSVPMSLYGAVDGHTARSHQAQRDTAEPIRM
                     WIRIRIRIRIRIDGTVIPMR"
     gene            8267..8815
                     /locus_tag="IE241_RS08965"
                     /old_locus_tag="GCM10010324_18360"
     CDS             8267..8815
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE241_RS08965"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010324_18360"
                     /product="hypothetical protein"
                     /protein_id="WP_190021137.1"
                     /transl_table=11
                     /translation="MLYVFGFERIGVVASDMYFVDPDPIPGQEGAERGVRLEVRVLRPG
                     DLPGGIYASRPIVVDRPVWRADLLESAAGPSGSLDRAHHHPRFDGWEPGGRVFVDELSA
                     APLDWVAGRLADLDTLLAEADVPASEAGPDDAADLRNAVPDIMAAVRRLLDGVAKGELA
                     RPPAGLAEEDIADARVSWL"
     misc_feature    9178..9194
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 17.14"
                     /tool="antismash"
     gene            9178..9927
                     /locus_tag="IE241_RS08970"
                     /old_locus_tag="GCM10010324_18370"
     CDS             9178..9927
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF047266.1"
                     /locus_tag="IE241_RS08970"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_18370"
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="WP_229898993.1"
                     /transl_table=11
                     /translation="MSQEDILSVVEGTTLAVHVPGFVHPEALASAQRQLFDHPDRGSLS
                     QAAEFKRIGFAYSEISDGTSRDRYHAEARTNIQRMRDLFAPYASPSDVLRLLLEETWAA
                     GAGLMHVEGRKAFIGICRYQDKNVDLNPHTDALERNLPAEHARRLKAQLSVNIYINIPR
                     TGGELELWGTEPGEDAYHRLMGGRTWGIDRETIGPPVEVVKPAPGDLLLLNPRQIHAVR
                     PSGDEPRITLGHFLGYYGTDRPLELWS"
     assembly_gap    9976..9996
                     /estimated_length=21
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9997..10739
                     /locus_tag="IE241_RS08975"
                     /old_locus_tag="GCM10010324_18380"
     CDS             <10001..10739
                     /GO_function="GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /gene_functions="biosynthetic (rule-based-clusters) CDPS:
                     CDPS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016572541.1"
                     /locus_tag="IE241_RS08975"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_18380"
                     /product="tRNA-dependent cyclodipeptide synthase"
                     /protein_id="WP_229898995.1"
                     /sec_met_domain="CDPS (E-value: 5.1e-37, bitscore: 122.4,
                     seeds: 17, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="NGTDDTARINTGINAGIDGRSKASFGEKAKSLPLAGKRAVLLVSV
                     GQRYHEGDKLRATVELVQRGGFQQVTIAVADTLQRTNYLGISRQDAYDRALKAGDEWLQ
                     RNEKTLARLAAISDVLRWDEALRDPVYPQFRRRVEDAYEHDLRYRQALLRTVGKFLDRL
                     TARDPEADIAVASRNCLGYLIEECPVIMPTWAHRGYDYVIYPQPMTVAMAETRRLFVED
                     AYPGKAEWLPLKFKKRAMTAPHVI"
     misc_feature    10271..10273
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    10607..10609
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            10817..12370
                     /locus_tag="IE241_RS08980"
                     /old_locus_tag="GCM10010324_18390"
     CDS             10817..12370
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1005: Drug
                     resistance transporter, EmrB/QacA"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539434.1"
                     /locus_tag="IE241_RS08980"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_18390"
                     /product="MFS transporter"
                     /protein_id="WP_190021139.1"
                     /transl_table=11
                     /translation="MTASTDRKWWAFAGVSLISFLGCIDLTIVNTAAPQIQADLGASVT
                     DLQLIVNMFIVALSMFMVTMGRLADIYGRRRVLYTGTVVFALASLGAGLAPGTGWLIAF
                     RFLQGAAGAVLYTSTGAIVQNAFPRSQRGRAIGALYGVNGLGLAVGPLLGGVIVSSVGW
                     HWVFWLNVPLIAVALTLCSFSVKESRDQGAPGLDWPGLVLISLGIPAVVLAFTLGDHWG
                     WSSARTLGLLAFATACLVAFYAVERRAADPIIAFRLFRHRAFLGAIVSDFALACFYATV
                     LFLLPLYLSDVRDYGGYATGALLLPCTAVMAVLSPGVGRLTDRYGPRKLLAVGFAAFTV
                     SALLLARIAPDTSIGYLALALVAMGLGWAFILGPATVSALSSVPERQAGLAVGSSWTFH
                     NLGGAIGLAAAINLYRLQSEHTLTTALAERHAPTGPWTQSVVGDPEHATALLRQHTSLS
                     GPEIGEVYGQVFSHGMQSAMWMVAGTSLAALAVLGLLGLSARRSTAAACEVTVDNELAR
                     "
     gene            complement(12393..13010)
                     /locus_tag="IE241_RS08985"
                     /old_locus_tag="GCM10010324_18400"
     CDS             complement(12393..13010)
                     /EC_number="2.3.1.-"
                     /GO_function="GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006127829.1"
                     /locus_tag="IE241_RS08985"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_18400"
                     /product="N-acetyltransferase"
                     /protein_id="WP_190021140.1"
                     /transl_table=11
                     /translation="MQSTNTPKNTPKPIESGDAPEITRTLARAFGDDPMMLWLFPDEDG
                     REGRLAQWFDVMLTHKYGPLGHCERTDAAAAFWTPPGTAEQHPDEGTLARINGLLGEHA
                     ARLGEMLELVGEATPREPHWYLAVLGADPAAQGSGHGAALLLSGLAKADAAGLPAHLES
                     SKQANIGFYERFGFTVRGEIHVPGGGPTLWSMWREARADVRR"
     gene            13366..14895
                     /locus_tag="IE241_RS08990"
                     /old_locus_tag="GCM10010324_18410"
     CDS             13366..14895
                     /EC_number="3.4.11.-"
                     /GO_function="GO:0004177 - aminopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014175235.1"
                     /locus_tag="IE241_RS08990"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_18410"
                     /product="M1 family metallopeptidase"
                     /protein_id="WP_190021141.1"
                     /transl_table=11
                     /translation="MRHRSTVVGGALAACLLLTVPASAATPTPGSAGIGDPYLPDSGNG
                     GYDVSHYDLRLQYRPRTDRLDGTATLLATATQDLSRFDLDFVLDVQEVLVDGKRAAFAK
                     AGAHELQVTPRTPLSKGRPFTVVVRYGDVPSEKKVDGHSAWSRTPDGAVVAGEPAAAAW
                     WFPSNDHPRDKATYDISVAVPEGLQTVSNGILSSRSTQRGWTRYTWRSTKPQATYLATL
                     AVGKFEIAQGTTSNGLPVINAYSKDLGDGLAPAKASIERTGEVIDWASTLFGPYPFEAA
                     GGYVPNLPGRGALETQTRPVYASGTFQNGAFMEVVVHELAHQWFGDSVSVRNWRDTWLS
                     EGFATYAEWLWSEKEGEGTARELADYAYASQPADDPFWKVRPADPGKDNPLHNAVYLRG
                     AMALQALREKIGDSTFFTVLKEWQKQRRHGNATVEDFTRLAQDVSGKSLRRLFDTWLYT
                     PGKPAQGPGAAGGKNGIAQAAQPVQPKSWKNLERARLSHRHRPELTGADRS"
     gene            15067..18969
                     /locus_tag="IE241_RS08995"
                     /old_locus_tag="GCM10010324_18420"
     CDS             15067..18969
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1173: WD-40
                     repeat-containing protein"
                     /gene_kind="other"
                     /inference="COORDINATES: protein motif:HMM:NF044287.2"
                     /locus_tag="IE241_RS08995"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_18420"
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="WP_268254364.1"
                     /transl_table=11
                     /translation="MDGKGQASGRDQAGASGPAAPQAGELLRRLRRERGMTLDGLARQA
                     FYSKGYLSKVENGEKPLTLELARVCDRVLTAGGVLEQLVRRPAGGRRKAQTEDVCPYRG
                     LAPFGTGDARWYFGRDEAVADVVSQLTERLRIPGTLLLMAPSGAGKSSLLRAGLPPALA
                     RGVLPASGSQAWPVVLFTPGEHPLEELLDRIAVATGASRGLLGKALEEGGGALAATVRA
                     ALDSLQQAADSGAGTGQASQDSVAPGCARKAASLVVVVDQFEETFMLCPDEKEQTAFIE
                     ALGALAEGRSEESEERDGISPALVVLGVRADFYDRCLAFPGLAASLRRGHVTLGPMDDA
                     QLRTAITGPAHAAGLEVESGLVEILLRDIGLTPGDAKHSGANVNRAKALPLLSHALLST
                     WQHRKDTTLTVDGYQLTGGISGAVAATAERAYASLPARRQEIARQVLLQLIQVGEDRET
                     SRPRRRTELLEAGEDVIETFTRARLLTVDTDHVEVAHEILLHVWPRLRQWIDDDRTSLR
                     IRQTLADAATAWQNEDRDTGLLYRGTRLAAALEAAAIPNGTALLTPAARTFLEASAGLE
                     ASEKLTERRRLRRLRILTSGLATLLVLSLVAGALAFQQNREADAQRRIAVSRELAARAD
                     GMLRDRPEAAMLLALGAYRRSPTAEARSSLLSAYAQFPGNQLTGHTDDVYGVAFSPDGR
                     TVATAGNDHSAKLWDRASQRLIATLAGHTDQVVTVAFSPDGRILATAGRDRTAKLWDTS
                     DHRLIATLTGHTRGVSGVAFSPDGRALATAGDDGTVRLWDVTGQRPTAVLAGGNSKAWR
                     VAFSPDGHTLAATYHDKVARLWDTASHREVATLTGHTDTVTGVAFSPDGHTLATASADA
                     TVRLWDARTHRVLTTLTGHKQSVMSVAFSSDSRTLATASYDGTTRLWDTATHRTTGTLA
                     ETDTPRPGFPVSVAFSPDNKYLVTGTVWGAGGNGNAPSPQLWDASTQRKAGSFTGTDNA
                     VTAVALSPDRRTLAAGDSGGMVTLWNTAARSPFAALTGHTEAITSLAFSPDGRTLASTS
                     SDRSARLWDMSTHKEIAALTGHTSKILTAAFSPDGRTLATAGSDRTTRLWDTRTHRAIA
                     VLGKEPDAVSGLSFSPDGHTLATGNYDATARLWDTRSHKLITTLPHTGQTCSIVSLAFS
                     PDGRTLAATSCDAVLWNAETHKYVTSLSGHTAAPKQLAYSPDGRTLATAGLDRTIRLWD
                     TRDRHAVATLTGPGAFISALAFSPAGDFLATANTDPTIRLWELNPEVVADRVCDLSRSH
                     RWHQAIADLPADTPCP"
     gene            complement(19106..19276)
                     /locus_tag="IE241_RS09000"
                     /old_locus_tag="GCM10010324_18430"
     CDS             complement(19106..19276)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE241_RS09000"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010324_18430"
                     /product="hypothetical protein"
                     /protein_id="WP_190021143.1"
                     /transl_table=11
                     /translation="MTFAVFVVFALERYVLCMCLRFPSAHVAGAWAAALVMRTSGVPRA
                     AARVMAAAAAS"
     gene            19367..19711
                     /locus_tag="IE241_RS09005"
                     /old_locus_tag="GCM10010324_18440"
     CDS             19367..19711
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543114.1"
                     /locus_tag="IE241_RS09005"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_18440"
                     /product="hypothetical protein"
                     /protein_id="WP_190021490.1"
                     /transl_table=11
                     /translation="MRARDKTAVSALRATLAALDNAEAVPVDEAALRGGAFEQSPVGAG
                     ATEAPRRELSERCMIDVVRAEATERLEVAAQLTAPAHADRAAQLRAEAAVLLHFLDSAW
                     TGPGTGPGTT"
     misc_feature    complement(19878..19892)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.99"
                     /tool="antismash"
ORIGIN
        1 gagcgatccg ccatgaaagc ccccgccatc gccccctgcc tcggtgtgaa ggacacccag
       61 gcgtccgtgg acttctacga gaagatcggc ttcaccgcgc tgcccgcggg cgacgacccg
      121 aacgacgaca tccgctcgct gctcttcggc gaggacttcg cgctgatggt ctaccgcgac
      181 gcccacctgc gggactggct gccggtcctg aaggaccgca cgcccggcgc gttcggcatg
      241 ttctacctgg ccgtcgacga cttcgccggc tacgtcgcca agatccggcc cctggtgaac
      301 gccaccaagg aagccaccca caacgggcag aacctcttct acttcaccga ccccgacggc
      361 tacgtcatcg gcgtcgtcca gaagcaggag tggtgaaccc catgaccacc accaaggcct
      421 cgaccccggt cgcgaaggtc gcctcgtacg ccttctgggc ccgtgacggc aaggagctcg
      481 ccaggttcta cgccgccgcc ctgggcttcc agctcgtcca ggagtacccc gacgagcagg
      541 gcgtgcccac cgcgttcctg gtcagcaatg ccgacggcag tgtgctctac accttctaca
      601 ccgcccgtga ttaccgtgct cccgcctggc cccaggacga gttgcctttc cacctcgacc
      661 tgtccttcga cgacgtcgag gccggtgaga agcgactgct ggagttgggc gcgaccaagc
      721 cggaccacca gcccggcggc ggtcagtgga ccgtgttgct cgacccgtcg ggtcagccgt
      781 tctgcatcag cgccgccgcc aactggcagt actgacaccc cagggcgccg ggccgccctg
      841 gcctgtggcc ccgcaccccg ccctcgccct cccgagcgcc ggccgggagg catccgaagg
      901 tggggctccc atgccgttcg cctcgtgttt cagcgtcaag gacaccgcgg tcaccaagcc
      961 ctgacggtca acgacaccaa ccacaactac gcctgaccgc cgctcgtgcc accgaaatac
     1021 tcaagcccct ctacctcacc ctcgtacggg tcctgggcca cgagcacttc aacctccacc
     1081 tggtccgctg accccctccg cagcgaaccg gaccgaatcc cccccccgga agccctcgca
     1141 cgatgactgc cctgcgccgt atcacccctg ccgaacgccg tgcccggctc gccgaacggc
     1201 acctgctcgc cccggcccac cgggccaccg caccgcagga ggtcgccgag gcgctcatag
     1261 gcctccacgc caccgacccc gccacggtct tcctctcggc cgccgcccgc atgaccgccc
     1321 ccgccaccgc gaccatcgac cacgccctct acgacgcgac gcccccgctc gcccgcatcc
     1381 ggtgcatgcg acgcaccatg ttcgtcaccc ccccaccacc tcgcgcccac cctccacgcg
     1441 gccaccgcca aggatgccca ccgcaaccgc ggcagcgccc tcgaccatct ccgcaccacg
     1501 ctcggctgga cccccgcccg ctacgccgcc gtggagaagg ccaccctcga tgctctggtc
     1561 gcccgtggca gggcttccgc cgccgagctc gccgccgaca tccctgacct gcgcgagggt
     1621 tacaccctca atgccggcaa gtcgtacgag acccgtcagc gcgtgaccgc cccgatcctg
     1681 ggaaccctcg ccgccgaagg ccgcatccgc cgcagccgcc cggccggttc gtggacctcg
     1741 gcccagttcc gctgggaacc cgccgaaccc ctccccgcca tccccgcggc cgaggcccaa
     1801 accatgctgg cccgccacta cctggccgcc ttcggcccgg tcaccgcagc ggacctgaag
     1861 tggtggacgg gctggacgct gaccgacacc cgcaaggcga tcgcagccac cggcgccgaa
     1921 gccgtcgaac tcgacgaagg caccggttac ctcctcgccg aggacaacca gcacctcgca
     1981 gagatcgacc acgaaccctg ggtggcactg ctgcccggct tggaccccac ctccatgggc
     2041 tggcgccacc gcgacttcta cctcgacccg caccacacgg cggccctgtt cgaccgcaac
     2101 ggcaacatag gccccaccgt ctgggccgac ggccgcatca tcggcgcctg ggcccagcac
     2161 cccgacggcc acatcaacca ccacctcctc accgaccccg gccgccacac ccgcgccctg
     2221 atcaacaacg ccatccagcg cctcgccatc ttcctcgacg gcacccgcgt caccccctgc
     2281 taccgcacac ccctcgaacg ccaactcgcg accacagccg ctcccgcaac ccgccacggc
     2341 tgagacgccg gcagtcctcc ggagcaagac acgtcccaac ggcctacctc cctggaaccg
     2401 ccgccgggcc cgcgcccggc ccgtcccacc cagcggtgcg gcccctcctg aggggcagtg
     2461 tcgcttcccc acagacccac acacagccac cgccctcccc tgcaacggct gagcagcggg
     2521 gagtgctacg gagtcaatcc cggcctcctg gcctgtgccc ggccgcggca ccgccctcgt
     2581 acggcactac cggaaagaag aacacctcca tgtccccctc ccggcactcc gtcacgcctg
     2641 ccgtacccgg cacgctccgc ggccgcggcc gcgcccgttg gacggcactg ggacgggctc
     2701 tgctccaacg aggcctcccc ggcccgggtc ggcacgtcat cctcctgatt cggccgcccg
     2761 cgggcagtgc tgccggcccc agagggatgt ccacccgccg gaccgtacgg ctgtcgtcgc
     2821 ccagcccgga ccgggccgcc acgtgggcac cggtgatcgt cgggctcatc gggctcggca
     2881 tcggactgcg ggggatcacg cgcccttccg catggaccga cgagatcgtc accctggacg
     2941 tggcacggcg gtcctggccg cagctggtgg agctgctcgg acgcgtggac gcggtgcacg
     3001 gcctgcacta cgtactgatg tacctggtcg ggcaagttgc cggggtcagc gagttcacga
     3061 tgcgtctgcc ctcagcggtg gcggtagccg tcgcggcagc cggcctcgga tggctggggc
     3121 ggctgctcgg aggcccgcgc ctcggcctgt acgcggggct gctgctcgcc gtcctgccca
     3181 ccgtgtcgcg ctacgcacag gaggcccgct cgttcgcctt cgtcatggcg gtcgcggtac
     3241 tggccacggg cgcactggtg aaggcgctgg ccggccgtgc ccggtgggcg gccggctacg
     3301 ccgtgctgat cgccctgctg ggctggttca acgtgatggg gctgctgctg gtggccgccc
     3361 acgcggtcac gatcgccctg cggcgtcccg gccgccgcgt agtcatgcgc ctcctgccgg
     3421 cccaggccgc gggcatcacc gccgtcattc cgctgctgct cctgggcttc tcccagcgct
     3481 ccgccgtggg cgaggttcca caggtcaccg ccggaacccc gtacagctac ttcacctggc
     3541 tgctcacccc cggccaggac accctccccg ccgccctgaa actcctgctc accctcacgt
     3601 ccctcgccgc gatatccctc ctcgtggccc gccggcacac ggccccgccc ctcgtcttcg
     3661 ccatcggcgt gccctggctg gccgtgcccc cgctgctcct cctggccctc tccctcggtc
     3721 agcccctctt cgcctaccgc tacctgctgt tctgcctccc ggccctggcc ttgctgctcg
     3781 cagcggccgc cgccctcctc ccgccgcgtc ggcaactgct gctcgcactc ctgccggccc
     3841 tcccgatcac catgtcccac ctcgccatac gccagcagga cagccgtccc tgggacacca
     3901 gcaccgtgat caccaccctg cgcagccgcg ccgtccccgg ggacgccatc ctcttcagcg
     3961 gcgcccggtg cgacctgatc gccaccaccc accccgaaat cttcgcccgc ctccccgacc
     4021 tgggcacggc ccggacagcc gccgaacgcg gcactctgaa caacctgccc gccgactcca
     4081 ccctcctgaa ccagcgcctg gcccgtgcgc cccgcgtctg gcacatcacc tgccgccatc
     4141 tttcccccgg cgcttaccgg accggcgacc gcacgaccgt tgcccaggaa tacgccctca
     4201 cccgcactgg ccacaccccg gcctaccacc gcagcacccg cggcctggac atcacccttt
     4261 accaacgctg acccgccacc gggcggtcgc cggtggaccc gtccgccgag cccggggcag
     4321 cgagagcaac ccggcggccc ggccggtgat gccggcacac aaacaccgcc cttgcggcgc
     4381 cgcggcagtc cgggggcggt ccggctggtc gtggcagatg cgctgcatcc aggtggccgg
     4441 ggttgcgacc ttcgagaact tgctgcacca cggcggcagg atcgccgagg agaaccgccg
     4501 gggcgcagct cctcgacgag gcccgcggcg gcgagggcgg ccagcgtggt gccgcccaga
     4561 aaggcggcgc cgagttccgc ggccgtcagc cgcaggtcgg ctggggcgtt cgtacgctcg
     4621 cagctgacgt cggcgccgtc ggcgcggagc cggtgggggg cagtgttcca cgggcataag
     4681 tcgtcccgta cgtctaggac gaggtcgagc gacgccaacc agtcgtatcc acagccggtc
     4741 cacgacggtc gaccggaccg cccgggactc ggccagcaga tgtggcagcg gctcgtcggt
     4801 cgcgccctcg tagtcgatcc aggtcaccaa gtcgagcctg gcgaggaaga accacagcgc
     4861 ggcgtacgcc tgacgcgaga gcgccgccag ttccaccacc cgcaccacac cggtgccgcc
     4921 ggacggaccc cccggtggca tccttaccgg tccgaccagc cgaccgtctc ggcccgcgcc
     4981 cggtcgtaga tctgctcgat gaccaggcgc gttcgcggcc cctcagtcga gacagaactc
     5041 gttgccctcg atgtcctgca tcacgatgca cgactcgttc tcgtcatcgg caggcagcag
     5101 tcgcacgcgt accgcgccga gcgcgatcag tcgtgcggac tcggcctcaa gtgtggcgag
     5161 gcgttcttca cccacaagcc cggtgccgac ccgcacgtca agatgaaccc gattcttgac
     5221 gaccttgcct tcgggaacgc gctggaagta cattcgcggg cccacacctg agggatccat
     5281 gcaggcgaac gctgacccct ggctctcagg cggcagcgag cgattgaaat cgtcccaagt
     5341 agcaaatccc tccggcggcg gcggtacgac gtaccccaac acctcgcacc agaaacgagc
     5401 gacgcgctca ggttctgcgc agtcgaaggt gacttggaac ttcttgatcg acgccatcgt
     5461 tccaccatag tggcgcgtgc tgttcaccga aaccccggag tgtcatcgcc gcgcgggtca
     5521 gtgaaggtga accacgccga ggctatgacc accagtgggt catcttgacc gagacagggt
     5581 gcggccccgc gttacggtca atctgacact cgtgagccct gtgctcgtac ccggtcaggc
     5641 acgccgtcag catattgctt gctccggccc gccggccccg tccagacgcg actgcgaccg
     5701 ggcggtcgat acgctcctgc catcagcgcg gtaccgacta ctcaacggcc ttagggacag
     5761 gtgaagttga agtgaccgca caagcgatgg cgatggcgat ggcgatgtta caggcggccg
     5821 gagccgcaga gatcgagcac cccggaggca cgctgctggc acatcttcag cgggtcagcg
     5881 ccctcctctc cgcgtggggc gctcgcccgg cgctggtgtc ggccgggctg tgtcacgcgt
     5941 tctacgggac cgacggcttt cccgtcgctc tgctggacct gggccgccgg gccgagctgg
     6001 ccgaggcgat cggcaccgag gccgaggcac tcgtctactt ctacgcctcc tgcgaccgga
     6061 agtcctcgta ccgcggactg accgatgaca ccggcctgtt caccgaccgg ttcaccggca
     6121 cccggacgca tccaggcctc gaagcgcgtc gggacttcgc cgagctcacc gccgcaaacg
     6181 agctggacat cgccgcgatc agcccacaga tgcgcgcccg ccacggagcg gacctgctcg
     6241 cgctgttcac ccgatggcgg ccgctgctgt ccgagccggc ctggacacac tgccgcagcg
     6301 tgctgggcca ggccgaccac tgatcggccg ggaccgagcg gtgtcacggt caccagcagc
     6361 gcggcgagca ggggaaacgc gagagcacga gggcccaggt ggtgtcgttt cacgggatct
     6421 gatctccttc gccggcggta ggtctgcggg ctgttacaca tcacccccga ggggggtccg
     6481 gaccctcgcc gccgggcgca ctgccgctga cgacgacggt tgcacgcagc atcgcaatct
     6541 ccggagcgcc cgcgtggcga tggccatccc gacggcctga acagcaacgc tcggccattc
     6601 gggtgacatc ggctgacatc tccgcgccta cagcaggcca aggtcgtctt cgggcccgtg
     6661 aaccgttgaa ccgcgaaggc tcgcgaggtg ctccacgccg tggcaaggcg ggcccccggc
     6721 gccgatgcgc cgggagcccc gccgtcgctc atccgtggcg cggtcagccg taggcgagcg
     6781 ccttgagcct gttgtacacg ccgttgaagc ggtcatggtc gccgactgtg gggccggtgt
     6841 cggtgtgctg ccagaaggtg tagaaacccc agccgttcgg gagctcaccg gaggaggagc
     6901 cgtagcgcgg tacccacagc gggttgtact tgccgaatcc gccgtagtta ccggtgcact
     6961 tcttccacca cgcggtcgag gtgtagatca ccgcgtcgcg cccggtgtgg tcccagtagg
     7021 cgtcgaggaa gtcgtcgatc cagtcgatca tccggcccgt gctcagcccg tagcaggggc
     7081 tgccgtaggg gttgtactcc atgtcgagca cgcccggcag cgtccagccg tcgtcggtcc
     7141 agccgccgcc gtggtggacg aagtagtcgg cctgtgcggc gccgccggag ttgcccggca
     7201 gcgcgaagtg gtacgcgccc cggatcatgc ccgcgtcgta cgggccgttg tactgctggg
     7261 cgaagtgggg gttggtgtag ctggtgccct cggtcgcctt ggagtaggcg aatctgacgc
     7321 cgctgttcca gagcttggac caggagacgt tgccgttgtg gctgctgacg tccacgcctt
     7381 tgaccccggc gctccccgag gggccctccg gggagacgtc gggcagcgag gggtcgggca
     7441 gcgaggagcc cttggtggcc gggctgcgtt cgccctcgtg ggcgcggacg gtcgaaccca
     7501 tccagtcctg ctcggggtgg atcggactgc tactgctccc ggcgctgttc ccggtgctgc
     7561 tcccggccgc cggggcagcg gttccgggga gggtgacgag caaggtgagt gcggtcagca
     7621 gggagaggag gacggccgcc gctgtggggg gatgcgggcg gcccgggccg gagtggtgct
     7681 cggacatgag gtgcctccga ggccatggtg ggggacggtt cggggggcaa cggtcaccgc
     7741 atgggtatga ccgtgccgtc tatccggatg cggattcgga tacggatgcg gatccacatc
     7801 cggatgggct cggcggtgtc acgctgggcc tggtgagagc gggccgtgtg cccgtccacg
     7861 gcgccgtaca ggctcatggg cacgctgacc aggccggacg tggggacctc cgaccagatc
     7921 gatccggcca cacgatcgcc tcctccggca ctcggctcca agctgcactg ccacgttcac
     7981 atccaggccg cggcagcgca cggggggaca ggcagccgga tgacccttgg ggtacatggg
     8041 ccgctatggc acgtgagcgg ctccgggggg tggccggtcg tggtggaccg ggcgaggcag
     8101 atcgtgggcg ggacgtgctc tcggtcgccg agtcgaagaa tctggtgacc ttcgggggcc
     8161 gcccgggcag tcacccgtgg cgcgatcaga ccctctggcg cacctgcgcg cgtcggcccc
     8221 accctgagga caacggttcc gtcggtcggg acatccggga gggcacatgc tgtacgtctt
     8281 cggtttcgag cggatcggcg tagtggcgag cgacatgtac ttcgtggatc ccgacccgat
     8341 cccgggtcag gagggcgccg aacggggtgt acgtctggag gtccgcgtcc tgcggccggg
     8401 ggacctgccg ggaggcatct acgcgtcgcg gcccatcgtg gtcgaccgcc cggtgtggcg
     8461 ggccgacctc ctggagtccg cggcgggccc gtccggcagc ctggaccggg cgcaccacca
     8521 cccgaggttc gacggctggg agcccggcgg acgcgtcttc gtcgacgaac tctccgccgc
     8581 accgctggac tgggtggccg gcaggctggc ggacctcgac acccttctgg cggaggccga
     8641 cgtcccggcc tccgaggcgg gtccggacga cgccgccgac ctccgcaacg ccgtccccga
     8701 catcatggcg gcggtgcgca ggctgcttga cggcgtggcc aagggggagc tggcccgtcc
     8761 gcccgccggc ctggccgagg aggacatcgc ggacgcccgt gtcagttggc tgtgacggtc
     8821 tgcccgaacg cgctggcacg gtctgggtgg cggcgccatg aggcgggcgc cgccgaccgg
     8881 acaagacccg gctcaggggc gtgaacgcgc aggcgcgcgc ccatggtggg catcaggggt
     8941 gcgctccctc ccccgcgacg gaggcgccgc gcggtcgtct ccgccgtcgg gaggaaccgc
     9001 cggtggacct gcctcatcca agagggcgcc catgaatcgt tacacagcat gaggatccag
     9061 taattaacgg tcgagatgat ggcattacct gcaaaacgcc ccgcgcctca ctgtcggtca
     9121 cgcgggccgc acacacgggg gaccatcatc aacatcaagc gcaccacctc atgccagttg
     9181 tcccaagagg acatcctctc ggtcgtcgag ggcaccactc tggccgtcca cgtcccgggg
     9241 ttcgtgcacc cggaggcgct cgcctcggcg cagcggcagc tcttcgacca ccccgaccgg
     9301 ggcagcctga gtcaggccgc ggagttcaag cgcatcggat tcgcctactc cgagatcagc
     9361 gacggtactt cgcgcgatcg ctaccacgcg gaggcgcgca ccaacatcca gcggatgcgc
     9421 gatctgttcg cgccctacgc ctcgcccagt gacgtcctgc ggctgctgct ggaggagacc
     9481 tgggccgccg gggccggctt gatgcacgtc gaaggccgca aggcgttcat cggcatctgc
     9541 cgctaccagg acaagaacgt cgacctcaac ccgcacaccg acgcgcttga gcggaacctg
     9601 cccgccgagc acgcgcgccg cctcaaggcg cagctctcgg tcaacatcta catcaacatc
     9661 ccgcgcaccg gtggagagct ggagctgtgg ggaacggagc ccggcgagga cgcctaccac
     9721 cgcctcatgg gcgggcggac ctggggaatc gaccgcgaga cgatcggccc gcccgtggag
     9781 gtcgtcaagc cggccccggg cgatctgctc ctgctcaacc cgcgccagat ccacgccgtg
     9841 cgcccgtcgg gggacgagcc ccgcatcacc ctggggcact tcctcggcta ctacggcacc
     9901 gaccgcccgc tcgaactgtg gagctgacgg tgaacaacga cgtcaaaagc atcgagcacg
     9961 gcatcaacga aaacgnnnnn nnnnnnnnnn nnnnnnccaa cggcaccgac gacacggctc
    10021 gcatcaacac cggcatcaac gccggtatcg acggtcgcag caaggcgagt ttcggggaaa
    10081 aggccaagtc gctccctctc gccgggaaac gggccgtcct gctggtgagc gtcggccagc
    10141 gctatcacga gggcgacaaa ctccgggcca ccgtggaact cgtccaacgc ggcgggtttc
    10201 agcaggtgac catcgccgtg gctgatactc tgcagcgcac caactatctc gggatttccc
    10261 ggcaggacgc ttacgaccgc gcactcaagg cgggagacga gtggctgcag cgcaacgaga
    10321 aaacactcgc ccggctcgcc gcgatcagcg atgtccttcg ctgggacgag gcgctgcgcg
    10381 acccggtcta cccacagttc cgccgacgcg tcgaggacgc ctacgagcac gacctgcgct
    10441 accggcaggc cctgctccgt accgtcggaa agttcctcga tcgcctcacg gctcgcgacc
    10501 cggaagcgga tatcgccgtg gcgtcccgga attgtctggg ctacctcatc gaggaatgcc
    10561 ccgtcatcat gccgacctgg gcacaccgcg gatacgacta tgtgatttat ccgcagccca
    10621 tgacggtggc catggcggag acccgacggc ttttcgtcga ggacgcatac cccggcaagg
    10681 cggagtggct tcccttgaag ttcaagaaac gagccatgac cgccccgcac gtcatctaga
    10741 ccgcatccag accgaatcca gatcccgtcc agcccccatc cagaccgccc ttgacccggt
    10801 gtaggggaac atcaccgtga cagcatcgac cgaccgcaag tggtgggcat tcgccggtgt
    10861 cagcctcatc agcttcctcg gctgcatcga cctcaccatc gtcaatactg ccgccccaca
    10921 gatccaggcc gatctcggcg cctccgtgac cgacctccag ctcatcgtca acatgttcat
    10981 cgtcgccttg tcgatgttca tggtgacgat ggggcggctg gctgacatct acggccggcg
    11041 tcgcgtcctc tacaccggaa ccgtcgtctt cgccctggcc tccctcggcg ccggactcgc
    11101 cccgggcacc ggctggctca tcgccttccg gttcctccaa ggcgccgcgg gagccgttct
    11161 ctacacctcc acgggcgcca tcgtccagaa cgcctttccc cggtcgcagc gcggccgtgc
    11221 catcggcgcc ctctacggcg tcaacggcct gggcctggcc gtcggcccgc tgctcggcgg
    11281 agtcatcgtc tcctccgtgg gctggcactg ggtgttctgg ctgaacgtcc ccctcatcgc
    11341 cgtcgccctg accttgtgct ccttcagcgt caaggagtcc cgcgaccagg gcgcccccgg
    11401 tctggactgg cccgggctcg tgctcatcag cctgggcatc cctgccgtgg tcctggcctt
    11461 cactctcggc gaccactggg gctggtccag cgcccgcacc ctcggcctgc tggccttcgc
    11521 gacggcctgt ctcgtcgcct tctacgccgt cgaacgccgt gcggccgacc ccatcatcgc
    11581 cttccgtctc ttccgccacc gcgccttcct cggcgccatc gtctccgact tcgccctggc
    11641 ctgcttctac gccaccgtgc tgttcttgct gcccctctac ctcagcgacg tacgggacta
    11701 cggcggttac gccacgggtg cgctgctcct gccgtgcacc gccgtcatgg cggtgctctc
    11761 ccccggcgtc ggccggctca ccgaccgcta cggacccagg aagctcctgg ccgtcggctt
    11821 cgccgcgttc acggtctcgg ccctgctgct cgcgcggatc gcccctgaca cctccatcgg
    11881 atacctcgcc ctcgcgctgg tggccatggg cctcggctgg gcgttcattc tcggtcccgc
    11941 caccgtctcc gcgctgtcct ccgtgcccga gcggcaggcg gggctcgccg tcggatcctc
    12001 ctggaccttc cacaacctcg gcggagccat cggactggcg gctgccatca acctctaccg
    12061 ccttcagagc gaacacacgc tgaccaccgc actcgccgaa cgccacgcac ccaccggccc
    12121 ctggacgcag tccgtggtcg gcgacccgga gcacgccacg gcgctgctcc ggcagcacac
    12181 ctccctcagc ggcccggaga tcggcgaggt ctacggccag gtcttctccc acggcatgca
    12241 gtccgcgatg tggatggtgg ccggcacctc actggccgcc ctcgccgtcc tcggtctcct
    12301 cggcctgtcg gcacgccgct ccacggccgc cgcctgcgag gtgacggtcg acaacgaact
    12361 cgcccggtaa gccgccgccg ggcgcccccg gcctaccggc gaacgtctgc gcgcgcttcc
    12421 cgccacatcg accagagtgt cgggccgcct ccgggtacgt ggatctcgcc gcgcacggtg
    12481 aacccgaatc gctcgtagaa gccgatgttg gcttgcttcg acgattcgag gtgggccggc
    12541 agtccggctg cgtccgcctt ggccaggccc gaaagcagca gggcggcgcc gtggccgctg
    12601 ccctgggccg ccgggtcggc gccgagcacc gccaggtacc agtggggttc ccggggagtc
    12661 gcctcgccga ccagttccag catttccccc agccgggccg cgtgctcgcc cagcagcccg
    12721 ttgatgcggg ccagcgtccc ctcgtccggg tgctgctcgg ccgtgccggg cggtgtccag
    12781 aaggccgctg ccgcatcggt gcgttcacag tgcccgagcg ggccgtactt gtgcgtgagc
    12841 atgacgtcga accactgggc gagacggccc tcacgaccgt cttcgtcagg gaacagccac
    12901 agcatcatcg ggtcgtcccc gaaggcgcgg gccagggtgc gtgtgatctc aggagcgtcg
    12961 ccgctctcga tcggcttggg cgtgttcttg ggggtgttcg ttgactgcat gccgacaacg
    13021 taggttccga gatctttcca gcgcatcggt cgttctaccg atagtccggg ttccgccgag
    13081 agtccatgcc cccggggcga cagtccaggc ccccggggcg ctggccgctc gacagcacaa
    13141 tcggtgcgac gagaccccgc aagggcgccg tagcacgggg tttccctgcc cgaggccgag
    13201 gaggaaacag gaaacctctc gacctcgggg acccgttcct gtgtagtgat ggccagggcc
    13261 cgcagcagtg cccgaccaac aggtccaacc gacgttgccg ctgcctcgcc ctttcccatt
    13321 gtgccgagca ctgccttact cattgcatca ggggaagtga ttcgattgcg ccaccgatcc
    13381 accgtcgtgg gcggcgctct tgccgcctgc ctcctcctca ccgttccggc ctcggccgcg
    13441 acgcccacgc ccggatccgc cgggataggg gacccgtacc ttcctgattc cgggaacgga
    13501 ggttacgacg tctctcacta cgacctgcgt ctgcagtacc ggccaaggac cgatcggctg
    13561 gacggcacgg cgacgctcct tgccaccgcc acccaggatc tgagccgctt cgacctggac
    13621 ttcgtcctcg acgtccagga agtcctggtc gacggcaaga gagccgcctt cgccaaggcc
    13681 ggtgcacacg agctgcaggt cacgccacgt accccgctgt ccaaggggcg cccgttcacc
    13741 gtcgtggtcc gctacggcga cgtcccgtcg gagaagaagg tcgacgggca ctccgcgtgg
    13801 tcccgtacac cggacggagc cgtggtcgcc ggggagcccg ccgcggccgc ctggtggttc
    13861 cccagcaacg atcacccccg ggacaaggcc acctacgaca tctccgtcgc cgtaccggaa
    13921 ggcctccaga ccgtaagcaa cggcatcctc agctcccggt ccacccaacg cggctggacc
    13981 cgctacacct ggcgctccac caagccccag gccacctacc tcgccaccct ggccgtcggc
    14041 aagttcgaga tcgctcaagg caccacctcc aacggcctgc cggtcatcaa cgcgtacagc
    14101 aaagacctgg gtgacggact cgcaccggcc aaggcgagca tcgaacgcac cggcgaggtc
    14161 atcgactggg cgagtacgct cttcggcccc taccctttcg aggcggccgg cggctacgtg
    14221 ccgaacctgc ccgggcgcgg cgcactggag acccagacac ggccggtgta cgcctccggg
    14281 acgttccaga acggtgcctt catggaagtc gtcgtgcacg aactggcgca ccagtggttc
    14341 ggtgacagcg tctcggtgcg caactggcgc gacacctggc tcagcgaggg cttcgccacg
    14401 tacgcggagt ggctgtggtc ggagaaggag ggcgagggga cggcccggga actcgccgac
    14461 tacgcctacg cctcccagcc cgccgacgac ccgttctgga aggtccggcc tgcggacccc
    14521 ggcaaggaca acccgctcca caacgcggtc tacctgcgcg gggccatggc actccaggcc
    14581 ctgcgcgaga agatcggcga cagcaccttc ttcaccgtgc tcaaggagtg gcagaagcag
    14641 cgccgccacg gcaacgccac cgtggaggac ttcacccgcc tcgcccagga cgtctccggc
    14701 aagtcgctgc gccggctctt cgacacctgg ctttacaccc ccggcaaacc ggcgcagggc
    14761 ccgggggcgg ccggcggcaa aaacgggatt gcccaggcgg cccagcctgt tcagccgaag
    14821 tcctggaaga atctcgagcg ggctcggctc agccaccggc accgaccgga gctgaccgga
    14881 gctgaccgga gctgaccggc acacaggtca cctgtggtga gggaatcgtt gcgctacggc
    14941 accacgcgct gaaaagatct tccttggtat tgcagccgca tccggtgggg gcctgggcac
    15001 gcttgagccg ggaagccctc gatgggcgag cccccgctgg gacgtgccag gagggggtga
    15061 tcggcgatgg acgggaaagg ccaggcttcc ggcagggatc aggccggggc gtcgggcccg
    15121 gctgcgccac aggccgggga gctgttgcga cggctgaggc gtgaacgggg catgacgctg
    15181 gacgggctgg ctcgccaggc gttctacagc aagggctatc tgagcaaggt cgagaacggt
    15241 gagaagccgt tgacgctgga gctggcccgt gtctgcgacc gggtgctgac ggcgggcggg
    15301 gtgctggaac aactggtccg gagaccggcc ggaggaagac gcaaggcaca gaccgaggac
    15361 gtctgcccgt atcgggggct ggctccgttc ggcaccgggg atgcccggtg gtacttcgga
    15421 cgcgacgaag ccgtcgccga cgtggtctct cagctgaccg aacggctccg cataccgggg
    15481 acgttgctgc tcatggcccc gtccggagcg gggaagtcct cgttgctcag ggcagggctg
    15541 ccgcctgccc tggcacgcgg ggtcctaccc gcctccggct cgcaggcctg gcctgtcgtg
    15601 ctgttcacgc cgggcgagca ccctcttgag gagctcctcg accgcatagc ggtggcgacc
    15661 ggagcatccc gcgggctgtt gggcaaggcc ttggaggaag gagggggcgc tctggccgcc
    15721 accgtgcgcg ccgcgctgga cagccttcag caggctgcgg attcgggcgc cggcacggga
    15781 caggcgtcgc aggactcggt cgcacccggc tgcgctcgta aggccgcttc ccttgtcgtc
    15841 gtggtggacc agttcgaaga aaccttcatg ctctgccctg acgagaagga gcaaacggcg
    15901 ttcatagagg cgctgggggc tctggcggaa ggacggagcg aggagagcga agagagagac
    15961 ggcatttctc cggcccttgt cgtcctcggg gtccgcgccg acttctatga ccgctgcctg
    16021 gccttccccg gactggccgc gtcgctgcgg cgaggccacg tcacgctggg gccgatggac
    16081 gacgcccagt tgcgtacggc catcaccgga ccggcgcacg ccgccggctt ggaggtggaa
    16141 tcggggctgg tcgaaatcct gctccgtgac atcggcctca cgccgggtga tgcgaagcat
    16201 tccggcgcga acgtgaacag ggccaaagcg cttcccctgc tctcccacgc actgctcagc
    16261 acctggcagc accgcaagga caccacgctg accgttgacg gctaccagct gaccggcggc
    16321 atctcaggcg cagtggccgc cacggccgag cgcgcctacg cctctctccc cgctcgccga
    16381 caggagatcg cccggcaggt cctgcttcag ctgatccaag tcggagaaga cagagagacc
    16441 agccggccca ggcgacgaac cgaactgctg gaggccggtg aggacgtcat cgaaacgttc
    16501 acccgggccc gcctgttgac ggtggacacc gatcacgttg aggtggccca tgaaatcctg
    16561 ctgcacgtct ggccccgcct gcgccaatgg atcgatgacg accgcaccag tctgcgcatc
    16621 cgccaaaccc tcgcggacgc ggccaccgct tggcagaacg aggaccgcga caccggcctg
    16681 ctctaccgcg gcacccgcct cgccgcagcc ctcgaagccg ccgcgatccc caacggtacg
    16741 gctctcctga cacctgcggc gcggaccttc ctcgaggcaa gcgccggtct cgaggcgtcc
    16801 gagaaactga cggagcgccg acgtctgcgt cgcctgcgca tcctcacctc cggcctggca
    16861 accctgctcg ttctctcgct cgtggccggt gccctcgcct ttcagcagaa ccgggaagcg
    16921 gacgcccaac gccgcatcgc ggtgtcccgg gaacttgccg cccgggccga cggcatgctg
    16981 cgagaccggc cggaggccgc catgctcctc gccctcggcg cctaccgccg ctcccccact
    17041 gccgaggccc gcagcagtct gctcagcgct tacgcccagt tccccggcaa ccagctcacc
    17101 gggcacacgg acgacgtcta cggggtggca ttcagccccg acggccgcac ggtggccaca
    17161 gccggtaatg accactcggc caaactctgg gaccgggcct cgcagcgtct catcgcgacg
    17221 ctcgccgggc acaccgatca ggtcgtcacc gtggccttct cgccggatgg ccgtatcctc
    17281 gccacggccg gcagggaccg gacggcgaag ctgtgggata cgagcgatca ccgtctcatc
    17341 gccacgctca cgggccatac caggggtgtc agcggagtgg cgttcagtcc tgacggacgc
    17401 gccctggcaa cggccggcga cgacggcacc gttcgcctgt gggatgtcac cggacagcgg
    17461 ccaacagccg tccttgccgg cggaaacagc aaggcttggc gggtcgcgtt ctcacccgac
    17521 ggccacacgc tggcagccac ctaccacgac aaggtcgcca ggttgtggga cacggcttca
    17581 catcgggaag tcgcaaccct caccggacac accgacaccg tgacgggtgt cgcgttctcc
    17641 cccgacgggc acacactggc cacagcaagc gccgacgcga cggtgcgtct gtgggatgcg
    17701 cgaacacatc gtgtcctcac caccctgacc gggcacaagc agtccgtcat gtcggtggct
    17761 ttctcctctg acagccgtac cctcgccact gccagttacg acggcaccac acggctctgg
    17821 gacaccgcca cgcaccgcac caccggcacg ctggcagaaa cagacacgcc tcgaccgggg
    17881 ttcccggtga gtgtggcttt cagcccggac aacaagtatc tggtgacagg gacagtatgg
    17941 ggagcgggtg gcaatggcaa cgctccaagc cctcagttgt gggacgcctc aacccagcgg
    18001 aaggccggat ctttcacggg caccgacaac gccgtcacgg ccgtggctct ttcccccgac
    18061 cggcgcaccc tggccgccgg cgactccggc ggaatggtca cgctgtggaa caccgccgcc
    18121 agaagcccgt ttgccgccct caccggacac accgaagcca tcaccagcct ggcgttctca
    18181 cccgatggcc gcaccctcgc cagcaccagc agcgaccgca gcgcgcggtt gtgggacatg
    18241 agcacgcaca aggagatcgc cgccctcacc ggacacacct caaagatcct caccgcagcc
    18301 ttcagccccg acggccggac cctggccacc gccggcagcg accgcaccac gcgcctatgg
    18361 gacacacgca cacaccgtgc catcgccgtc ctcggcaagg aacccgacgc cgtctccggc
    18421 ctgtccttct cccccgacgg acacaccctc gcgaccggca actacgacgc gaccgcccgc
    18481 ctctgggaca cccgctccca caaactcatc accaccctgc cccacaccgg ccagacgtgc
    18541 agcatcgtct ccctcgcctt cagccccgac ggccgcacac tcgccgccac cagctgcgac
    18601 gccgtgctgt ggaacgcaga aacccacaag tacgtcacca gtctctccgg tcacacagca
    18661 gccccgaagc aactggccta ctctccggac gggcgcacct tggccacagc cggtttggac
    18721 cgcaccatcc gcctatggga cacgagggac cggcatgcag tggccaccct gaccggaccg
    18781 ggcgccttca tatccgccct ggccttttcc cccgcaggcg acttccttgc caccgcaaac
    18841 acagacccca ccattcggct atgggaactc aacccggagg tcgtcgctga tcgcgtctgc
    18901 gacctcagcc gaagccaccg ctggcatcag gccatcgccg acctccctgc cgatacgccc
    18961 tgcccctgag ccttgtggcc cacgctccac ggccagggct gctgccatta cggcgaccgc
    19021 cgtggccgcc cggccagccc ggcgacgggg atgccggcca cggcatcgag cacgtggtgg
    19081 tagcggcagt agacgaccgc gacggtcacg acgcggccgc ggctgccatg acgcgcgcag
    19141 ctgctctcgg cacgccgctc gtgcgcatga cgagggcagc ggcccaggcg cccgccacgt
    19201 gggctgaggg gaagcgcagg cacatgcaga ggacgtagcg ctccagcgcg aacaccacga
    19261 acaccgcgaa cgtcacagca cgttcacgtt gtgggcccag gggcacagcc ataaggtcac
    19321 cggtatgtct gtacgtctgc gtatgcgtca agcactgccg gaagcgatgc gtgcccgtga
    19381 caagaccgca gtgagcgcac tgcgcgcaac acttgccgcg ctggacaacg ccgaggcggt
    19441 gcccgtggac gaagccgcgc tccgcggcgg ggcctttgag cagtcgccgg tcggcgccgg
    19501 cgccaccgaa gcaccacggc gtgagctgag cgagcgctgc atgatcgacg tcgtgcgcgc
    19561 cgaagccacc gagcggctgg aggttgcagc gcagctgact gcacccgcgc atgccgaccg
    19621 agccgcgcag cttcgcgcgg aggccgccgt gctgcttcac ttccttgaca gtgcctggac
    19681 cggtcccggc accggtccag gcaccacata ggacaccgca ctagcgcacg gcggccgccc
    19741 gcaccgcggc ctcggccagc cggtggttct cggggcccgc gccgaacccc gacgggaagc
    19801 cgaagcgccg gcgcgtgtag gcataggcca cgccgctgtc cgggtccgcg aagccgagcg
    19861 agccggtcgc accgctgtga ccgaaggcat ccgggccgag gaacggatag cggccgctca
    19921 gctcctcgaa gcccagaccg aagacatcct cgttaccggt gaccaggtcg gttccgcggt
    19981 actgaacgcg ggcgaactcg gtgacggtct ccggcttcag cagcggagcc cggccgtcca
    20041 cctcactgat cgccgcggcg tacatccccg ccacgccccg tgcggtgccg accccgccgg
    20101 ccgacgtgga gccgagtgcc cggacggcac gggtgttggc gaaggccacc aggtcggtcg
    20161 gcggggtcgc gttctgattg aaggcgatgc ccttgacact gtccggggca gccgcgttgg
    20221 ccgcgagccg ctcggcctcc tccggagtgg gcagcatcgg cagcacgtcg gcatagcgcg
    20281 cttcgagcga ctcgggcagg cccaggtgga agtcgagccc gtacggcgcc cggatccgct
    20341 cttcgtagat ctcctggatc gagcggccgg tcacccgtcg cacgacctcg cccgtcaggg
    20401 cgccgatcac gaaggcgtga tagccgtacg ccttccccgg ctcccagaag ggccgctgcg
    20461 cggcgagccg cgcggccgtc agccggtcgt cggccagctc ctctgtggtg aaaccgccgt
    20521 cgacgcccac gaccccggaa cggtgcgcga gcagctcgcg cagggtcagc cggcccttgc
    20581 cctccgcccc gaactcccgc cagtacgcgg ccacttcgcg gtccagatcg aggacgccgt
    20641 cctgcaccag gagcgcgacc acgaggtggg cggcgccctt ggtgatggag tagaggccgg
    20701 tcagcgtgtc cgccgcagta ccgggaccgc cccacaggt
//
