LOCUS       NZ_BMUT01000002        10405 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces hiroshimensis strain JCM 4586 sequence02, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUT01000002
VERSION     NZ_BMUT01000002.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces hiroshimensis
  ORGANISM  Streptomyces hiroshimensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 769107)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 90X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650335.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:32:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,830
            CDSs (total)                      :: 6,745
            Genes (coding)                    :: 6,620
            CDSs (with protein)               :: 6,620
            Genes (RNA)                       :: 85
            rRNAs                             :: 1, 3, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 2 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 125
            CDSs (without protein)            :: 125
            Pseudo Genes (ambiguous residues) :: 0 of 125
            Pseudo Genes (frameshifted)       :: 25 of 125
            Pseudo Genes (incomplete)         :: 107 of 125
            Pseudo Genes (internal stop)      :: 4 of 125
            Pseudo Genes (multiple problems)  :: 11 of 125
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 17:07:11
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 302280
            Orig. end                         :: 312685
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUT01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="melanin"
                     /region_number="1"
                     /rules="melC"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="melC"
                     /kind="single"
                     /product="melanin"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="melC"
                     /neighbourhood="5000"
                     /product="melanin"
                     /protocluster_number="1"
     gene            complement(1409..2443)
                     /locus_tag="IE241_RS06695"
                     /old_locus_tag="GCM10010324_13800"
     CDS             complement(1409..2443)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007264107.1"
                     /locus_tag="IE241_RS06695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_13800"
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="WP_190020693.1"
                     /transl_table=11
                     /translation="MAGARTKNRTKKRLRMRAGPTAVAAAVCALAATAAFVLPTGGAPA
                     QGPPARAGAPAAPAPGAPAPEAAAREAAAPQQCTDPEASLRPSGGTDGPAVQRIKARGR
                     LIAGVDQNSYRWGYRNPADGELSGFDIDLVRAVAKDILGDPGKVTYLAIPTSRRISALQ
                     TGKVDIIARTMTITCDRVRQVAFSTAYFESGQQVMVPKGSPVTAFDDSLKGKKVCTSEG
                     STAEIKVREEPHGSSLLTVPNQLDCLVRLQLGEVDAIVNDRALGAGQAAQDPSVQLVGK
                     PFTTEPYGVAMNLADTDLVRRVNKVLEDYRGGGAASLWMKAYEKWLAADLKGITAPPAP
                     KYRD"
     gene            complement(2488..3879)
                     /locus_tag="IE241_RS06700"
                     /old_locus_tag="GCM10010324_13810"
     CDS             complement(2488..3879)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060740.1"
                     /locus_tag="IE241_RS06700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_13810"
                     /product="hypothetical protein"
                     /protein_id="WP_229898877.1"
                     /transl_table=11
                     /translation="MSQPQPAGGPRGAPVRKPAAPAWREALERVRAAAVTEPGRLRVIG
                     AVLAVALVAFGAVTAWQVASRSAAADSVVRTSQPLSADAADIYRSLADADTTAASGFLA
                     GGQEPRAVRQRYEKDIALASRLLVKASANAEGSAAARARIATLNEGLPRYTGLVEAARA
                     NNRQGLPLGGAYLRYAGDRMREELLPAARGLYEAETGRLGSDYDDAKSWPWAALALGAV
                     VLGALAWAQRRNYLRTNRVFNHGMLAATAAAVVVLLWLVVGHAVARSGLETSYAHGARS
                     LQVLNEARIGSLQARGDENLTMVARGAVLGKNGDDAYETSYRDGMGRLVGGSQGPARAD
                     TLLDRARALADDAEGRDPVDAAIRDVREWQQRHVQARTLADDRGDYDDALRKVIGDKDS
                     TGESFDRVDTELQRALAHEQSEFRQAADDGRGAFFGLAAGAAVLTVLGAAGAVVGVGRR
                     LSEYR"
     gene            complement(4118..4939)
                     /locus_tag="IE241_RS06705"
                     /old_locus_tag="GCM10010324_13820"
     CDS             complement(4118..4939)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091617.1"
                     /locus_tag="IE241_RS06705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_13820"
                     /product="tyrosinase family protein"
                     /protein_id="WP_190020694.1"
                     /transl_table=11
                     /translation="MAVRKNQANLTATEKRNFVNAVLELKRTGVYDGFVRTHNEFIMSD
                     TDNGDRVGHRSPSFLPWHRRFLIQFEQELQKIDSSVTLPYWDWTADRTATASIFAADFM
                     GGNGRSRDAQVTTGPFAYSTGKWTINVSVDNRNFLQREMGAAGTLPTRAQVDAVLAMPV
                     YDTAPWNSASEGGFRNYLEGWRGAGRVHNNVHVWMGGQMATGVSPNDPVFWLHHAHIDK
                     LWSDWQRMHPQSAYLPAAGTANVVDLNDTMRPWNNVTPAQMLDHSSYYTYA"
     gene            complement(5001..5405)
                     /locus_tag="IE241_RS06710"
                     /old_locus_tag="GCM10010324_13830"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     melanin: melC"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003969.1"
                     /locus_tag="IE241_RS06710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_13830"
                     /product="tyrosinase cofactor"
                     /protein_id="WP_190020695.1"
                     /sec_met_domain="melC (E-value: 7.1e-44, bitscore: 144.8,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSNVSRRVVLRGTAAAAAGVALLATAAQATDHTGHTTTEDHTGH
                     GGHGTGPAAFDEVYKGRRIVGKPVTGGHEAHHGGFAVTIDGKELHVMQNADGSWISVVN
                     HYQVFDQPLALTRAAVDALRGATLVPIAMN"
     misc_feature    5513..5527
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.39"
                     /tool="antismash"
     misc_feature    5598..5609
                     /note="TFBS match to AfsR, Pleiotropic regulatory for
                     antibiotic production, confidence: medium, score: 19.59"
                     /tool="antismash"
     misc_feature    complement(5629..5643)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 19.94"
                     /tool="antismash"
     misc_feature    5725..5740
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: medium, score: 16.26"
                     /tool="antismash"
     misc_feature    5741..5758
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.23"
                     /tool="antismash"
     misc_feature    complement(5743..5758)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 21.35"
                     /tool="antismash"
     misc_feature    complement(5744..5758)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.14"
                     /tool="antismash"
     gene            complement(5908..6939)
                     /locus_tag="IE241_RS06715"
                     /old_locus_tag="GCM10010324_13840"
     CDS             complement(5908..6939)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014060739.1"
                     /locus_tag="IE241_RS06715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_13840"
                     /product="N-acetylglucosamine kinase"
                     /protein_id="WP_190020696.1"
                     /transl_table=11
                     /translation="MGVSGALLAIDAGNSKTDVVLAGPDGAVLGAARGGGFQPARAGVR
                     QAVDGLAALVERAAAAAAGAAPAADPATAAAAASPPYVAHVSACLANCDLPVEEELLTA
                     EIAARGWGRTVAVANDTFALLRAGLPDGGEPVGVAVVCGAGINCAGLGPGGRTARFPAI
                     GRLSGDWGGGACLAEEALWSAARAADGRGGPSELARALPAHFGLDGMPALIEALHLGRV
                     AAERRHELVPVLFAVAAAGDAVARALVTRQAEEVVAMAAVALERLGLLGAGVPVVLGGG
                     VLAARHPLLHEQVVALLAGRAPRAVPVVVTAPPVLGAALLALDRAGALAAAYARLRAHY
                     GDA"
     gene            complement(6930..8183)
                     /locus_tag="IE241_RS06720"
                     /old_locus_tag="GCM10010324_13850"
     CDS             complement(6930..8183)
                     /EC_number="3.2.1.86"
                     /GO_function="GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014179641.1"
                     /locus_tag="IE241_RS06720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_13850"
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="WP_190020697.1"
                     /transl_table=11
                     /translation="MKLAVVGGGSTYTPELVDGFARLRDTLPVTELVLIDPDQGRLELV
                     GALARRILARQGHPGRVTWTADLDAGVDGADAVLLQLRVGGQAARQQDETWPLECGCVG
                     QETTGAGGLAKALRTVPVVLDIAERVRRRSPDAWIVDFTNPVGIVTRALLAAGHRAVGL
                     CNVAIGFQRKFAHLLGVAPEEVALEHVGLNHLTWERSVTVGGEDVLPRLIAEHGDAVAA
                     DLRMPRSVLERLGAVPSYYLRYFYGHDEVVRELRTKPSRAAEVAAMERQLLALYADPAL
                     AEKPALLAKRGGAFYSEAAVALTASLLRDTGDVQVVNTLNNGTLPFLPDDAVIEVPAAV
                     DAGGAHPLPVRPLEPLYAGLVANVTAYEHLALEAALAGGRDRVFRALLAHPLVGQFDHA
                     DRLTDALLAHNREHLAWA"
     gene            complement(8281..9444)
                     /locus_tag="IE241_RS06725"
                     /old_locus_tag="GCM10010324_13860"
     CDS             complement(8281..9444)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1108: ROK family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003982427.1"
                     /locus_tag="IE241_RS06725"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_13860"
                     /product="ROK family transcriptional regulator"
                     /protein_id="WP_190020698.1"
                     /transl_table=11
                     /translation="MNDRAALDLLVAHGPLTRTRLGELTGLSKPTASQLLTRLEAAGLV
                     RSTGSVTGRPGPSARLYEVDPGVAHVAALTVSPEGITALVADIAGTVVGEHHARVEEAE
                     EGAAKSDGTAARVARAVDGALAAARLGREALHRTVIGTPGALDPRTGVLRYAPHLPGWQ
                     SRTLRGELTAALGTPVVIENDVNLAAVAEQHAGAAQGCGDYVLVWADEGVGAAVVLGGR
                     LLRGATGGAGEIGYMPLPGAPLARGGDDATAGPDGGGGFQMLVASPAVVALARAHGIDA
                     RTAPEALARAAAEPGAGDDVYAELGRRLAVGLAAVVAVVDPELVVLSGAVCRAGGERLR
                     AMVEAELTGLALPRPQLRLSTVADRPVLAGALQTALAAARETVFDTA"
     gene            9613..10113
                     /locus_tag="IE241_RS06730"
                     /old_locus_tag="GCM10010324_13870"
     CDS             9613..10113
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146259.1"
                     /locus_tag="IE241_RS06730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010324_13870"
                     /product="FBP domain-containing protein"
                     /protein_id="WP_190020699.1"
                     /transl_table=11
                     /translation="MHPLADSDIRTSFINCSKGEARRIALPRNLAELPWEDLDFLGWRD
                     PGAPDRGYVVAEREGKLVGVTLRVPQGGPRSMAKSTMCSLCLTSHPGSGVSLLAARRVG
                     PAGREGNTVGAYMCADLACSLYVRGKKVPQMAMRYKETLSVEERISRMLVNLDDFLGKV
                     ERG"
ORIGIN
        1 cccgtgggct gctccccggt gcccgacggc gccgacagcg cctcggccag cgcctgctga
       61 tagcgcagca ccgcctgctc ggcggccatc agatcgcacg gcgcactcca cagcatgcgc
      121 cgcgccgcgt cgtaccgctc gatgagcagg gagtcctccg cgaggccgtg ccgggcggtc
      181 ttcgccttgt acgcgtccag gcggccgcgc agttcggcgc ggaccgccag cggggccgtc
      241 accgccgtca gcgactcgcg ggcgcgcagc agctcctcct ccgcgcgctg ctccaggccc
      301 tccaggagcg gcgagagccg gtgccaccgg gcgtgtctgc ggtgctcggc ggcggccgac
      361 agctgctcgt agagggccgt cggcgggccc gagaccgccg gcaccacgga cgcggcgatc
      421 ttcgacagga cctcgatgcg cgcctgccgt gcctcgttca gcgtgcggtc cgcgcgcgag
      481 aggacgtcgc gtacgcgcat cagccgctgc tcggcgtcct gccggacgtc cagcacggcc
      541 tcgacctccc ggcggacgtc ctccagggcc cgcgcggcgc ggtcgtagcg cgtggtgtcc
      601 ggacggccgc cgcccggggc cgagctgggc gggccgccgg cccagaagac gaccggatcg
      661 gatatcacct cttcgcgcag cgcggccagt tcccgggtga tctgctccag gtcgtcaccg
      721 gcggggtgct cgccgggccg gacgccgacc gagtgggcga gggagcgggt gcgcccgagc
      781 tcggcggcga gcaggtcgat ccgggcgggc agcgcggacc acacggcgtc ggcgatcacg
      841 acgacgtcga gcgcctgggc gtaccagccg ttcatccggt ccagcagctc ctgcaggctc
      901 aggtgctcgc tgagccgggc cggcatgccc gcagcgcccg ccgggcccgc cgcggccccg
      961 gtgaggcccg ccgtgccgag cacggccgtg ccgtggccgg gcagggtgat gccggagccg
     1021 cgcagcagct cggtgagctc ggacagctcg ccctgggtgg gccagcggcg gcgggcgcgc
     1081 agctcgcggg cggcggtcag ggcagcggtg taggcgtcga agtacgccca cagctgcccg
     1141 atgagggctt cggcggccgc ccaccggctg ctggtctccc cggtgagctc cgccccttcc
     1201 agcaggcgcc gccccgcgtg gtcctggagg gcgagcagcg aggattcgac ggcctcgtgc
     1261 tccgcgccga gcctggccag cgcacggtcc acctcctcgc gggtcaaggc cggcccggtg
     1321 ggctccgcga ccgccatcga tcccctctct cctgtccgcg gcgcgcgccg cgggcgtgtc
     1381 cgttacggcc gtgctccccg gcccgccgtc agtcccggta cttgggcgcc ggcggggcgg
     1441 tgatgccctt caggtcggcg gcgagccact tctcgtacgc cttcatccag aggcttgcgg
     1501 cgccgccgcc gcggtagtcc tccaggacct tgttgacccg gcgtaccagg tccgtgtccg
     1561 cgaggttcat cgccacgccg tacggttcgg tggtgaacgg ctttccgacg agctgcacgg
     1621 aggggtcctg cgccgcctgg cccgccccca gcgccctgtc gttgacgatc gcgtcgacct
     1681 cgccgagctg gagccgcacc aggcagtcga gctggttggg gaccgtcagc agggaggagc
     1741 cgtgcggctc ttcccgtacc ttgatctcgg ccgtggagcc ctccgacgta cagactttct
     1801 tccccttcag cgagtcgtcg aacgcggtca ccggtgaccc cttggggacc atcacctgct
     1861 gcccggactc gaagtaggcg gtggagaagg ccacttgcct gacccggtcg cacgtgatgg
     1921 tcatcgtgcg ggcgatgatg tccaccttgc ccgtctgcag ggcggatatg cgccggctcg
     1981 tggggatcgc gagataggtg accttgccgg ggtcgccgag gatgtccttg gcgacggccc
     2041 ggacgaggtc gatgtcgaag ccgctgagtt cgccgtcggc ggggttgcgg tagccccagc
     2101 ggtagctgtt ctggtcgacg cccgcgatca gccggccgcg ggccttgatg cgctgcacgg
     2161 ccggtccgtc cgtcccgccg gagggccgca ggctggcttc ggggtccgtg cactgctggg
     2221 gtgccgcggc ctcgcgtgca gcggcttcgg gggcgggcgc cccgggggcc ggcgccgcgg
     2281 gcgcgccggc ccgcgccggc gggccttgcg cgggtgcgcc gccggtgggc agcacgaacg
     2341 ccgcggtggc ggcgagggcg cacacggcgg ccgccaccgc cgtgggaccg gcccgcatcc
     2401 gcagccgctt cttcgtccgg ttcttcgtcc gggctcctgc catgtgcacc gcctccaccg
     2461 ccttcccctc gtccccctgc accggcctca ccggtactcc gacagcctgc ggcccacgcc
     2521 cacgacggca ccggcggcgc cgaggacggt cagcacggcc gctccggcgg cgaggccgaa
     2581 gaaggcgccg cgcccgtcgt cggccgcctg ccggaactcg ctctgctcgt gggcgagtgc
     2641 gcgctgcagt tcggtgtcca cccggtcgaa ggattcgccg gtgctgtcct tgtcgccgat
     2701 caccttgcgc agggcgtcgt cgtagtcgcc gcggtcgtcg gcgagggtcc gggcctggac
     2761 gtggcgctgc tgccactcgc ggacgtccct gatggcggcg tcgaccggat cgcgcccctc
     2821 ggcgtcgtcg gcgagggccc gggcgcggtc cagcagggtg tccgcgcggg ccgggccctg
     2881 gctgccgccg acgagccggc ccatgccgtc gcggtagctg gtctcgtagg cgtcgtcgcc
     2941 gttcttcccg aggacggcgc cgcgggcgac catggtgagg ttctcgtcgc cgcgggcctg
     3001 gagcgagccg atccgggcct cgttgagcac ctgcagggat ctggcgccgt gggcgtagga
     3061 ggtctccagg ccggagcggg ccacggcgtg gccgacgacc agccacagca ggacgacgac
     3121 ggcggccgcg gtggcggcga gcatgccgtg gttgaagacg cggttcgtcc ggaggtagtt
     3181 gcggcgctgg gcccaggcga gggcgccgag cacgacggcg ccgagggcga gggcggccca
     3241 cggccaggac ttcgcatcgt cgtagtcgga gccgaggcgg cccgtctcgg cctcgtacag
     3301 gccgcgggcg gcgggcagca gctcctcgcg catgcggtcg ccggcgtagc gcaggtaggc
     3361 gccgccgagc ggcaggccct ggcggttgtt ggcgcgcgcg gcctccacga ggccggtgta
     3421 gcgcgggagt ccctcgttga gggtggcgat gcgggcgcgg gccgcggccg agccctcggc
     3481 gttcgcggag gccttcacca gcagccggga ggcgagcgcg atgtccttct cgtagcgctg
     3541 ccggaccgcg cgcggctcct ggccgcccgc gaggaatccg ctcgccgcgg tggtgtcggc
     3601 gtcggcgagc gagcggtaga tgtcggcggc gtcggcgctg agcggctggc tggtgcggac
     3661 gacggaatcg gccgcggcgg atctggaggc gacctgccag gcggtgaccg cgccgaacgc
     3721 gacgagggcc acggccagga cggcgccgat gacccgcagc ctgccgggct cggtgaccgc
     3781 cgcggcccgc acgcgctcca gggcctcgcg ccaggccggg gcggcgggct tacggacggg
     3841 cgctcctcgc ggcccgccgg ccggctgcgg ctgtgacacg tgacctcccc ctcggtcgct
     3901 gagggcggcc cgtcacccgg cgtggccggg aacaggtgcc cggccagcag tatggccgca
     3961 agggctgaca aacacaccgg tgccggtgcg atcttgttcg tatcgcgatc ttcacccggg
     4021 gcccggggcg gctcttgttg cccatcacgc ccgccggccc ggtgcggttc cccgcaccgg
     4081 gccggacgcg tgtacgccct gtgcgtacgc ctgagcctta cgcgtacgtg tagtacgagc
     4141 tgtggtcgag catctgggcc ggggtgacgt tgttccacgg gcgcatggtg tcgttgaggt
     4201 cgacgacgtt cgcggtgccg gcggccggca ggtacgcgga ctgcgggtgc atgcgctgcc
     4261 agtcggacca cagcttgtcg atgtgggcgt ggtgcagcca gaagacgggg tcgttggggg
     4321 agacgccggt ggccatctgg ccgcccatcc acacgtggac gttgttgtgg acgcggcccg
     4381 cgccgcgcca gccctcgagg tagttgcgga agccgccctc ggaggcgctg ttccacgggg
     4441 ccgtgtcgta gaccggcatg gccaggacgg cgtccacctg ggcgcgggtg ggcagcgtgc
     4501 ccgcggcgcc catctcgcgc tgcaggaagt tgcggttgtc gacgctgacg ttgatggtcc
     4561 acttgcccgt ggagtaggcg aacgggccgg tggtgacctg cgcgtcacgg ctgcggccgt
     4621 tgccgcccat gaagtcggcg gcgaagatcg aggcggtggc cgtgcggtcg gcggtccagt
     4681 cccagtaggg cagcgtgacc gaggagtcga tcttctgcag ctcctgctcg aactggatca
     4741 ggaagcggcg gtgccagggg aggaaggagg gggagcggtg gccgacgcgg tcgccgttgt
     4801 cggtgtcgct catgatgaac tcgttgtgcg tccggacgaa gccgtcgtac acgccggtgc
     4861 gcttgagctc cagcacggcg ttgacgaagt tgcgcttctc ggtggcggtc aggttcgcct
     4921 ggttcttgcg cactgccatg gggttcgtgt cctcgtgagt gagaagtgtg gatcggtggg
     4981 gtgcgtgcgg cagtggccgg tcagttcatc gcgatcggga cgagcgtcgc gccgcggagc
     5041 gcgtccacgg cggcgcgggt gagcgccagc ggctggtcga agacctggta gtggttgacc
     5101 acgctgatcc aggagccgtc ggcgttctgc atgacgtgca gctccttgcc gtcgatggtg
     5161 acggcgaagc ccccgtggtg ggcctcgtgg ccgcccgtca cgggcttgcc gacgatgcgg
     5221 cggcccttgt agacctcgtc gaacgcagca gggccggtgc cgtggccgcc gtgaccggtg
     5281 tggtcctcgg tggtggtgtg gccggtgtgg tcggtggcct gggcagcggt ggcgagcagc
     5341 gccactccgg cggcggcagc ggcggttcct ctgagcacga cgcgtcggct gacgttggac
     5401 gacatgggtc ggtcccccct cgtatgcgcg ccggcacggc gcggaatagt cgatcagttg
     5461 cggaagttgt cacgaatgca gccgtaaatc ccttttgatg gcggttgctt gttgtatttc
     5521 gtgatcaatc cgtgcgggcg gatgctcgca tgatcgggaa gagggcgtga aacggttggc
     5581 cgcttttggg acattaggta aatgaacggt caacgaatct caggggagaa atttctgacg
     5641 aatgaccttg ttgacgccat gtcaggaaac ttggccgcta cgaggggggc tcggcgaaag
     5701 atcttccggc cggggccggg gtgcggtgac gtctctcacg atgtgaacat gacgtgaaga
     5761 aggcggcggt atcacggatt ccggccaggg aatgacagat tccgcgatac cggtcgtcac
     5821 gcgggaaaac aggcgccaaa cggacctcaa gccgcccgcc ccgctcgccc cgcgccccgt
     5881 tcgccccggg cggccccgcg gtccgaccta cgcgtcgccg tagtgcgcac gcagccgcgc
     5941 gtaggccgcc gccagagctc ccgcacggtc cagcgccagc agcgccgcgc ccagcaccgg
     6001 cggcgccgtg accaccaccg gcaccgcccg tggcgcccgc ccggccagca gtgccaccac
     6061 ctgctcgtgc aagagcgggt gccgggccgc gagcaccccg ccgcccagca ccaccggcac
     6121 cccggccccc agcagcccca gccgctccag cgcgaccgcg gccatggcga cgacctcctc
     6181 ggcctgccgg gtcacgagcg cgcgggcgac cgcatcgccc gcggcggcca ccgcgaacag
     6241 caccggtacg agctcgtggc gccgctcggc cgccacccgg cccaggtgca gcgcctcgat
     6301 cagcgcgggc atgccgtcca ggccgaagtg agccggaagc gcgcgggcca gctcgctggg
     6361 cccgccgcgg ccgtcggcgg cgcgcgccgc cgaccagagc gcctcctccg ccaggcacgc
     6421 gccgccgccc cagtcgccgg aaagccgtcc gatcgcgggg aagcgcgccg tacggccgcc
     6481 cggcccgagc cccgcgcagt tgatgcccgc cccgcacacc actgcgacgc ccaccggctc
     6541 gcccccgtcg ggcaggcccg cccgcagcag tgcgaaggtg tcgttggcga cggcgaccgt
     6601 ccggccccag ccgcgggcgg cgatctccgc ggtcagcagc tcctcctcca ccggcaggtc
     6661 gcagttggcc aggcacgcgc tgacgtgggc gacgtacggc ggtgaggcag cagccgcagc
     6721 cgtggccgga tctgcagccg gagccgcccc cgcggcagcg gctgcggccc gctccacgag
     6781 cgccgccagg ccgtccaccg cctgccgcac gcccgcccgc gccggctgga accccccgcc
     6841 gcgtgcggcc ccgagcacgg cgccgtccgg ccccgccagc accacgtcgg tcttgctgtt
     6901 gcccgcgtcg atcgcgagca gcgcgccgct cacgcccacg cgaggtgctc ccggttgtgc
     6961 gcgaggaggg cgtcggtcag ccggtcggcg tggtcgaact gcccgaccag cgggtgcgcg
     7021 agcagcgccc ggaagacgcg gtcccggccc cctgcgagcg cggcttccag cgccaggtgc
     7081 tcgtacgccg tgacgttcgc gaccagcccg gcgtacaacg gctccagggg gcgtacgggc
     7141 aaggggtgcg cgcccccggc gtccaccgcc gccggcacct cgatcaccgc gtcgtcgggc
     7201 aggaagggca gggtcccgtt gttgagggtg ttgacgacct ggacgtcgcc ggtgtcccgc
     7261 agcagcgacg ccgtcagggc gaccgccgcc tccgagtaga aggcgccgcc gcgcttcgcc
     7321 agcagggcgg gcttctcggc cagcgcggga tcggcgtaca gcgccagcag ctgccgctcc
     7381 atcgccgcca cctcggcggc gcgcgagggc ttggtgcgca gctcccgcac gacctcgtcg
     7441 tgcccgtaga agtagcgcag gtagtaggag gggacggcac cgaggcgctc cagcaccgag
     7501 cgcggcatgc gcaggtcggc cgcgacggcg tcgccgtgct cggcgatcag ccgcggcagg
     7561 acgtcctcgc cgccgacggt caccgaccgc tcccaggtga ggtggttgag gccgacgtgc
     7621 tccagagcca cctcctccgg ggcgaccccc agcagatggg cgaacttccg ctggaagccg
     7681 atggccacgt tgcacaggcc cacggcccgg tgcccggccg ccagcagcgc ccgtgtcacg
     7741 atgccgacgg gattggtgaa gtccacgatc caggcgtccg ggctgcgccg ccgcacccgc
     7801 tcggcgatgt ccagcaccac cgggaccgtg cgcagcgcct tggcgagccc gcccgcgccg
     7861 gtcgtctcct gaccgacgca gccgcactcc agcggccagg tctcgtcctg ctgccgggcc
     7921 gcctggccgc ccacgcgcag ctggagcagc acggcgtccg cgccgtccac gcccgcgtcc
     7981 aggtccgcgg tccaggtgac gcggccgggg tggccctgcc gggcgaggat ccgccgcgcc
     8041 agcgcgccga cgagctccag ccgcccctgg tcggggtcga tcaggacgag ctccgtcacg
     8101 gggagcgtgt cgcgcaaccg cgcgaagccg tcgacgagtt cgggggtgta ggtggatccg
     8161 ccgcccacca ctgcgagctt caacctcgac tcctttgccg gttgccaagc caatgccggt
     8221 tgccaaacca atgccggtca ccaagcccat gccggttgcc aagccgaagc ggccgccggc
     8281 tcacgccgtg tcgaagacgg tctcccgtgc cgccgcgagc gcggtctgca gcgcacccgc
     8341 caggaccggg cggtccgcca cggtgctcag ccgcagctgc ggccggggca gggccagccc
     8401 cgtgagctcg gcctccacca tggcgcgaag ccgctcgccg cccgcccggc acaccgcgcc
     8461 ggagagcacc accagttcgg ggtccaccac ggcgacgacc gccgcgagcc cgacggccag
     8521 ccgcctgccc agttcggcgt agacgtcgtc acccgcgccc ggttccgccg ctgcgcgggc
     8581 gagcgcctcc ggtgccgtac gggcgtcgat tccgtgggcg cgggcgagcg cgacgacggc
     8641 cggggaggcg acgagcatct ggaagccccc gccgccgtcg gggcccgcgg tggcgtcgtc
     8701 cccgccgcgc gcgagcggcg cgcccggcag cggcatgtag ccgatctcgc cggcgccgcc
     8761 ggtggcgccg cgcagcagcc ggccgccgag cacgaccgcg gcgccgacgc cctcgtccgc
     8821 ccacaccagg acgtagtcgc cgcagccctg cgccgcgccg gcgtgctgct cggcgacggc
     8881 ggcgaggttg acgtcgttct cgatgacgac gggcgtgccc agggccgcgg tcagctcgcc
     8941 gcgcagggtg cgggactgcc agcccggcag gtgcggggcg tagcgcagga cgccggtgcg
     9001 cgggtcgagg gcgccggggg tgccgatgac ggtgcggtgc agggcttcgc ggccgagccg
     9061 ggcggcggcc agggcgccgt cgacggcccg ggcgacgcgc gccgcggtcc cgtcgctctt
     9121 cgccgctcct tcctccgctt cctccacgcg cgcgtggtgc tcgccgacca ccgtgccggc
     9181 gatgtccgcg accagcgcgg tgatgccctc ggggctgacg gtcagcgcgg cgacgtgggc
     9241 gacgcccggg tcgacctcgt acagccgggc gctggggccg ggccgcccgg tgacgctccc
     9301 ggtggagcgc accaggccgg cggcctccag acgggtgagc agctgcgagg cggtgggctt
     9361 ggacaggccg gtcagctcgc cgaggcgggt gcgggtgagg gggccgtgcg cgacgagcag
     9421 gtcgagggct gcgcggtcgt tcatggcgcg cagtacgcgc ggtgtccccg gggtcccggt
     9481 catctcgtcc tgactgttag gaaactttcc tatcgatacg cgggaggcta gggacccgta
     9541 gcgccgggcg tcaatcgccc cgcccgaaaa tcatgcgacg atccgcccga accggtgtta
     9601 atttccctga ccatgcaccc cctcgccgac agcgacatac gcacgtcctt catcaactgc
     9661 tccaagggag aggcgcgccg gatcgccctc ccgcgcaacc tcgcggaact cccctgggag
     9721 gacctcgact tcctgggctg gcgggatcct ggtgcgccgg accgcggcta tgtcgtcgcc
     9781 gagcgcgagg ggaagctcgt cggcgtgacc ctgcgggtgc cgcagggcgg gccgcggagc
     9841 atggcgaagt ccacgatgtg ctcgctgtgc ctgacctcgc acccgggctc gggcgtctcc
     9901 ctgctcgccg cccggagggt cggtcccgcc gggcgcgagg gcaatacggt cggcgcgtac
     9961 atgtgcgcgg acctggcctg ctcgctctat gtgcgcggca agaaggtccc gcagatggcg
    10021 atgcggtaca aggagacgct gagcgtggag gagaggatct cccgcatgct cgtgaacctg
    10081 gacgacttcc tgggcaaggt cgaacggggc tagccgggta aggccgagcg gggccgggcg
    10141 gggccgtgcc gcgcaggacg gccccggccc acggctcctc gccgcgggcg ccggcccgcc
    10201 gtcacttgcc cagagtcgcc cccggcaccg ccggcggtat cggcgtcgtg gcctgggact
    10261 gcggggacgc cgggttcgcc agggcggacg gcgcggccac ggccgccatc gggtcgggcc
    10321 cggccccgtc cgtctccagg ttggcatcca gcgggcggcc gacgatacgg ataccctccg
    10381 cgtccagcgc ctgcttgatg cgcca
//
