LOCUS       NZ_BMUQ01000058        12348 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces mirabilis strain JCM 4551 sequence58, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUQ01000058
VERSION     NZ_BMUQ01000058.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces mirabilis
  ORGANISM  Streptomyces mirabilis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 12348)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 117X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650275.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:52:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,392
            CDSs (total)                      :: 10,311
            Genes (coding)                    :: 9,898
            CDSs (with protein)               :: 9,898
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 413
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 172 of 414
            Pseudo Genes (incomplete)         :: 329 of 414
            Pseudo Genes (internal stop)      :: 40 of 414
            Pseudo Genes (multiple problems)  :: 115 of 414
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:21:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 12348
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUQ01000058.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            complement(<1..5848)
                     /locus_tag="IE289_RS50820"
     CDS             complement(<1..5848)
                     /NRPS_PKS="Domain: CAL_domain (50-501). E-value: 1.1e-90.
                     Score: 296.0. Matches aSDomain:
                     nrpspksdomains_IE289_RS50820_CAL_domain.1"
                     /NRPS_PKS="Domain: PP-binding (634-697). E-value: 5.9e-11.
                     Score: 34.5. Matches aSDomain:
                     nrpspksdomains_IE289_RS50820_PP-binding.1"
                     /NRPS_PKS="Domain: Heterocyclization (741-1034). E-value:
                     1.1e-96. Score: 315.7. Matches aSDomain:
                     nrpspksdomains_IE289_RS50820_Heterocyclization.1"
                     /NRPS_PKS="Domain: AMP-binding (1206-1626). E-value: 9e-90.
                     Score: 293.1. Matches aSDomain:
                     nrpspksdomains_IE289_RS50820_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) FAAL_cd05931"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00881"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /locus_tag="IE289_RS50820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="WP_190174895.1"
                     /sec_met_domain="FAAL_cd05931 (E-value: 5.4e-195, bitscore:
                     638.5, seeds: 56, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 3.9e-10, bitscore:
                     28.7, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2.3e-43, bitscore:
                     136.7, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 6.3e-100, bitscore:
                     322.9, seeds: 400, tool: rule-based-clusters)"
                     /sec_met_domain="PF00881 (E-value: 8.5e-14, bitscore: 40.4,
                     seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTDVTAAVHSGTVVDMLRANAVRDPDAVAFSHLTFPADGPEHGRR
                     VSLSRAELDTRCCALAAALAAHELARHRVLILAPPGLDFIVGLFGTLYAGAVAVTCPPP
                     VSDESDPRTERAVRIASDAQVSAVVTTREIRDRFGELRRRFGDVPWIAVDDVEPAASGH
                     LAVPATDRGDLALLQYTSGATGGPKGVMISHDNLVHQLPQAAAIFQLPPGSNVVSWISP
                     YHALGIVVHLMLSQYLGGQAVFLVPEDFVGHPLRWLRAISDTPGPVLSCAPNFAFGLCA
                     DQISERERTGLDLSGWRTTLNTAERVHAHTMARFIEAYQPHGFRTAAMCPGFGMTEAMV
                     LTGQRRGRPLVLDVDAAELEQGRAVVLGEPDAVPDGGAAEGERRLRLVGVGQPGPYSEV
                     IVVDPESRRRLGEHEVGEVWIRGPVVCQGYWKRAELTEEMFGARLSDGEGPYLRSGDMA
                     FLHNGELVLCGRRKEMIIVRGRNLYPHDIESDSERAHPALRGAPAAAFSVNRDAGEELV
                     LVQSVPDADALPVPLPELARRIRSAVTAAHEVDVHHLVLVSPDQIPKTISGKIQRGECR
                     RRYLAGELETLLEPLHAGPPSASAPVRPVSGRSTGGSAVLPGLLAALDPSLRAGVISAD
                     LRRRLGDLLGVPTDDVPTAVPLVALGLESIRAMELRAVLERDVQVDLPLATFLRSTVDE
                     LTELMVHRLDGRHASAASEPFEPSEPSEPFEPFEPSEPFHAAPEHRLEPFRLTGLQQAY
                     LAGRRAGEERGDVATHLYLEIEAGDLDLRRLESALTVLVSRHDMLRAVVAEDGTQRVLP
                     ESDATPVPFSVTDCSTESGPRTAAAIREELSHEIRPIGQWPMWEVRALILPGDRIRLAI
                     SIDLLIADVASIRLFFDDWRRLYAGERLPPLRTTFRDCVNAHAVDPEDPEYAAARDYWL
                     RRVPELPEGPQLPWRRGATAPARWSRRAHRLPARSWSRIRQLALQHELTPTSVLLATFA
                     TVLGRWSTTDRFLLNLPLFSRRRVHPDVDALIGDFTSVVLLEVDLRGRPGIAELADRLH
                     RQLWRDLEHRAFDGVAVQRELAKARPATGVPHCPVVFASARDQGWDQHGRDAGTLGQNW
                     LGETTLSISQTPQVLLDHQVYENAGALEFNWDSPVDAFLPGVRDEMFDAYCRLLDSLVD
                     AAAWSGGVDVCGAGPDEFVARVDATDGAVPSASLADGVMGWAAREPDRVAVVTADGGVL
                     RYGELGLWASGVAETLLGPDADPGALIAVGMSKSAEQVVAALGVHLAGRAYLPVDVDLP
                     PRRRAELLAAAGCSVVLVRTGDDHQQWPDTVRVVAVEDCRDRARRLAESSAKPTDVAYV
                     LYTSGSTGAPKGVTVSHQAALGTCTDVCDRFGIGADDAVLGLSSLSFDLSVFDVFGVLG
                     AGGRLVLPRPGSQRDPEHWLELVAEHGVTVWNSVPAVAEMVVSHLGAGAGAGAGAGAGA
                     GDGTGDGHGAGGRQGAALAGIRVALWSGDWIPVGLPDRWREWSPACRLISLGGATEAAI
                     WSISYEIGEVDPEWDSIPYGRPLTNQSFHVLDDRLDHCPVWVVGELYIGGLGLAEGYWG
                     DPVRTAERFITHPVSGERLYRTGDLGRWRPDGTIEFLGRTDGQVKIGGFRIELGEIDAA
                     LAQHPAIARAVTTATGTRHHPYLTTFVVPDRARRPAPAPEAGGDGAEYESLLGAVITDS
                     AHRAAVTAAQRGRRADLTSPAVGLPDFLDPERTGHRWNRRRSHRRFAEAEVPLAELARL
                     LNCLRAVGAAHRPVPKYRYGSAGSLYPVRTYVYVHPGRVGGLPGGSYYYDPLTHRLRTV
                     RSGVEMPASIHLSMNQDAFSSAAFHLLLVGPMAEVEPMYGRRAHDFCLIEAGAMSQLLA
                     ESAIEGPLGLCQIGLLRDEKPLRTLFALGDDEVLLHSLLGGVGLPEPGEAMDAGVPSPR
                     AAGPAGPAGPAGPAGP"
     source          1..12348
                     /culture_collection="JCM:4551"
                     /db_xref="taxon:68239"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces mirabilis"
                     /strain="JCM 4551"
                     /submitter_seqid="sequence58"
                     /type_material="type strain of Streptomyces mirabilis"
     region          1..12348
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..12348
                     /SMILES="CC(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12348
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[<0:>12348]"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      <1..>12348
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     misc_feature    complement(161..163)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     CDS_motif       complement(944..1006)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50820_0013"
                     /evalue="6.70E-10"
                     /label="NRPS-A_a8"
                     /locus_tag="IE289_RS50820"
                     /protein_end="1635"
                     /protein_start="1614"
                     /score="31.1"
                     /tool="antismash"
                     /translation="FLGRTDGQVKIGGFRIELGEI"
     aSDomain        complement(971..2230)
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE289_RS50820_AMP-binding.1"
                     /evalue="9.00E-90"
                     /label="IE289_RS50820_AMP-binding.1"
                     /locus_tag="IE289_RS50820"
                     /protein_end="1626"
                     /protein_start="1206"
                     /score="293.1"
                     /specificity="substrate consensus: Cys"
                     /tool="antismash"
                     /translation="WAAREPDRVAVVTADGGVLRYGELGLWASGVAETLLGPDADPGAL
                     IAVGMSKSAEQVVAALGVHLAGRAYLPVDVDLPPRRRAELLAAAGCSVVLVRTGDDHQQ
                     WPDTVRVVAVEDCRDRARRLAESSAKPTDVAYVLYTSGSTGAPKGVTVSHQAALGTCTD
                     VCDRFGIGADDAVLGLSSLSFDLSVFDVFGVLGAGGRLVLPRPGSQRDPEHWLELVAEH
                     GVTVWNSVPAVAEMVVSHLGAGAGAGAGAGAGAGDGTGDGHGAGGRQGAALAGIRVALW
                     SGDWIPVGLPDRWREWSPACRLISLGGATEAAIWSISYEIGEVDPEWDSIPYGRPLTNQ
                     SFHVLDDRLDHCPVWVVGELYIGGLGLAEGYWGDPVRTAERFITHPVSGERLYRTGDLG
                     RWRPDGTIEFLGRTDGQVKIG"
     aSModule        complement(971..3625)
                     /domains="nrpspksdomains_IE289_RS50820_Heterocyclization.1"
                     /domains="nrpspksdomains_IE289_RS50820_AMP-binding.1"
                     /incomplete
                     /locus_tags="IE289_RS50820"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       complement(1085..1174)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50820_0012"
                     /evalue="4.90E-15"
                     /label="NRPS-A_a6"
                     /locus_tag="IE289_RS50820"
                     /protein_end="1588"
                     /protein_start="1558"
                     /score="47.6"
                     /tool="antismash"
                     /translation="PVWVVGELYIGGLGLAEGYWGDPVRTAERF"
     CDS_motif       complement(1658..1687)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50820_0011"
                     /evalue="4.10E+01"
                     /label="Cy3"
                     /locus_tag="IE289_RS50820"
                     /protein_end="1397"
                     /protein_start="1387"
                     /score="-1.7"
                     /tool="antismash"
                     /translation="LSFDLSVFDV"
     CDS_motif       complement(1769..1828)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50820_0010"
                     /evalue="2.00E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="IE289_RS50820"
                     /protein_end="1360"
                     /protein_start="1340"
                     /score="29.5"
                     /tool="antismash"
                     /translation="AYVLYTSGSTGAPKGVTVSH"
     CDS_motif       complement(2456..2506)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50820_0009"
                     /evalue="5.10E-07"
                     /label="Cy7"
                     /locus_tag="IE289_RS50820"
                     /protein_end="1131"
                     /protein_start="1114"
                     /score="22.5"
                     /tool="antismash"
                     /translation="ISQTPQVLLDHQVYENA"
     CDS_motif       complement(2642..2689)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50820_0008"
                     /evalue="8.40E-06"
                     /label="afterCy5"
                     /locus_tag="IE289_RS50820"
                     /protein_end="1069"
                     /protein_start="1053"
                     /score="18.4"
                     /tool="antismash"
                     /translation="DLEHRAFDGVAVQREL"
     aSDomain        complement(2747..3625)
                     /aSDomain="Heterocyclization"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE289_RS50820_Heterocyclization.
                     1"
                     /evalue="1.10E-96"
                     /label="IE289_RS50820_Heterocyclization.1"
                     /locus_tag="IE289_RS50820"
                     /protein_end="1034"
                     /protein_start="741"
                     /score="315.7"
                     /specificity="C activity: active"
                     /specificity="Active site motif: DLLIADV"
                     /tool="antismash"
                     /translation="EPFRLTGLQQAYLAGRRAGEERGDVATHLYLEIEAGDLDLRRLES
                     ALTVLVSRHDMLRAVVAEDGTQRVLPESDATPVPFSVTDCSTESGPRTAAAIREELSHE
                     IRPIGQWPMWEVRALILPGDRIRLAISIDLLIADVASIRLFFDDWRRLYAGERLPPLRT
                     TFRDCVNAHAVDPEDPEYAAARDYWLRRVPELPEGPQLPWRRGATAPARWSRRAHRLPA
                     RSWSRIRQLALQHELTPTSVLLATFATVLGRWSTTDRFLLNLPLFSRRRVHPDVDALIG
                     DFTSVVLLEVDL"
     CDS_motif       complement(2750..2809)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50820_0007"
                     /evalue="2.80E-10"
                     /label="Cy5"
                     /locus_tag="IE289_RS50820"
                     /protein_end="1033"
                     /protein_start="1013"
                     /score="32.1"
                     /tool="antismash"
                     /translation="HPDVDALIGDFTSVVLLEVD"
     CDS_motif       complement(2861..2917)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50820_0006"
                     /evalue="3.30E-08"
                     /label="Cy4"
                     /locus_tag="IE289_RS50820"
                     /protein_end="996"
                     /protein_start="977"
                     /score="26.2"
                     /tool="antismash"
                     /translation="LTPTSVLLATFATVLGRWS"
     CDS_motif       complement(3203..3247)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50820_0005"
                     /evalue="1.10E-03"
                     /label="Cy3"
                     /locus_tag="IE289_RS50820"
                     /protein_end="882"
                     /protein_start="867"
                     /score="12.3"
                     /tool="antismash"
                     /translation="RLAISIDLLIADVAS"
     CDS_motif       complement(3425..3478)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50820_0004"
                     /evalue="1.90E-08"
                     /label="Cy2"
                     /locus_tag="IE289_RS50820"
                     /protein_end="808"
                     /protein_start="790"
                     /score="26.5"
                     /tool="antismash"
                     /translation="LVSRHDMLRAVVAEDGTQ"
     aSDomain        complement(3758..3946)
                     /aSDomain="PP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE289_RS50820_PP-binding.1"
                     /evalue="5.90E-11"
                     /label="IE289_RS50820_PP-binding.1"
                     /locus_tag="IE289_RS50820"
                     /protein_end="697"
                     /protein_start="634"
                     /score="34.5"
                     /tool="antismash"
                     /translation="DLRRRLGDLLGVPTDDVPTAVPLVALGLESIRAMELRAVLERDVQ
                     VDLPLATFLRSTVDELTE"
     aSModule        complement(3758..5698)
                     /complete
                     /domains="nrpspksdomains_IE289_RS50820_CAL_domain.1"
                     /domains="nrpspksdomains_IE289_RS50820_PP-binding.1"
                     /locus_tags="IE289_RS50820"
                     /monomer_pairings="Acetyl-CoA -> Acetyl-CoA"
                     /starter_module
                     /tool="antismash"
                     /type="cal"
     aSDomain        complement(4346..5698)
                     /aSDomain="CAL_domain"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE289_RS50820_CAL_domain.1"
                     /evalue="1.10E-90"
                     /label="IE289_RS50820_CAL_domain.1"
                     /locus_tag="IE289_RS50820"
                     /protein_end="501"
                     /protein_start="50"
                     /score="296.0"
                     /specificity="Minowa: Acetyl-CoA"
                     /tool="antismash"
                     /translation="AELDTRCCALAAALAAHELARHRVLILAPPGLDFIVGLFGTLYAG
                     AVAVTCPPPVSDESDPRTERAVRIASDAQVSAVVTTREIRDRFGELRRRFGDVPWIAVD
                     DVEPAASGHLAVPATDRGDLALLQYTSGATGGPKGVMISHDNLVHQLPQAAAIFQLPPG
                     SNVVSWISPYHALGIVVHLMLSQYLGGQAVFLVPEDFVGHPLRWLRAISDTPGPVLSCA
                     PNFAFGLCADQISERERTGLDLSGWRTTLNTAERVHAHTMARFIEAYQPHGFRTAAMCP
                     GFGMTEAMVLTGQRRGRPLVLDVDAAELEQGRAVVLGEPDAVPDGGAAEGERRLRLVGV
                     GQPGPYSEVIVVDPESRRRLGEHEVGEVWIRGPVVCQGYWKRAELTEEMFGARLSDGEG
                     PYLRSGDMAFLHNGELVLCGRRKEMIIVRGRNLYPHDIESDSERAHPALRGA"
     CDS_motif       complement(4385..4447)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50820_0003"
                     /evalue="3.30E-04"
                     /label="NRPS-A_a8"
                     /locus_tag="IE289_RS50820"
                     /protein_end="488"
                     /protein_start="467"
                     /score="13.5"
                     /tool="antismash"
                     /translation="CGRRKEMIIVRGRNLYPHDIE"
     CDS_motif       complement(4529..4606)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50820_0002"
                     /evalue="1.30E-08"
                     /label="NRPS-A_a6"
                     /locus_tag="IE289_RS50820"
                     /protein_end="440"
                     /protein_start="414"
                     /score="27.2"
                     /tool="antismash"
                     /translation="VGEVWIRGPVVCQGYWKRAELTEEMF"
     CDS_motif       complement(5267..5326)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50820_0001"
                     /evalue="3.10E-07"
                     /label="NRPS-A_a3"
                     /locus_tag="IE289_RS50820"
                     /protein_end="194"
                     /protein_start="174"
                     /score="22.8"
                     /tool="antismash"
                     /translation="ALLQYTSGATGGPKGVMISH"
     gene            6087..7664
                     /locus_tag="IE289_RS50825"
                     /old_locus_tag="GCM10010317_104730"
     CDS             6087..7664
                     /NRPS_PKS="Domain: AMP-binding (30-429). E-value: 1.9e-87.
                     Score: 285.4. Matches aSDomain:
                     nrpspksdomains_IE289_RS50825_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433572.1"
                     /locus_tag="IE289_RS50825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_104730"
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase"
                     /protein_id="WP_190174896.1"
                     /sec_met_domain="AMP-binding (E-value: 1.5e-94, bitscore:
                     305.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDGFVPWPDGLAQEYRRDGYWLGRPLGDLFQESCARHADRVAVV
                     CGERELTYAELGDSAQRLAYGLLELGVRPSDRVVVQMPNIPEFVVLLLALLRIGAIPVL
                     ALPGYRRVEMAHVGAHSGAVAYAVKDEFGGFDFRRLAREVSFAGHVLVSGDAQEFVALD
                     ALAGAPRELPSVNPSDPALFLLSSGTTGLPKLIPRTHDELAYLMRATARAMGAGSDLVY
                     LAVNPVSHTAALACPGVLGSLLLGGKAVLTSSVRPDEVFPLIEREKVTVTSLVPAVLRL
                     WADSGRRHHDLSRLVLQIGSAPLDSALATRARKVLECGLTRWFGTTEGFLAHTRLDDPE
                     EVIENTEGRPLSEADEMLVVGPSGEPLPDGEAGELLVRGPYTIRGYYRAPEQNARSFTP
                     DGFYRTGDLACRRPDGNLVILGRIKDVINRAGEKVSPEEVERHLCAHPAVRDAAVVGVP
                     DRTLGERTYAFLLLRTDVTSAAVKTHLRERGLATYKIPDRIVTLADLPRTAMGKTDKEA
                     LRARARSS"
     aSDomain        6177..7373
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE289_RS50825_AMP-binding.1"
                     /evalue="1.90E-87"
                     /label="IE289_RS50825_AMP-binding.1"
                     /locus_tag="IE289_RS50825"
                     /protein_end="429"
                     /protein_start="30"
                     /score="285.4"
                     /specificity="substrate consensus: X"
                     /tool="antismash"
                     /translation="FQESCARHADRVAVVCGERELTYAELGDSAQRLAYGLLELGVRPS
                     DRVVVQMPNIPEFVVLLLALLRIGAIPVLALPGYRRVEMAHVGAHSGAVAYAVKDEFGG
                     FDFRRLAREVSFAGHVLVSGDAQEFVALDALAGAPRELPSVNPSDPALFLLSSGTTGLP
                     KLIPRTHDELAYLMRATARAMGAGSDLVYLAVNPVSHTAALACPGVLGSLLLGGKAVLT
                     SSVRPDEVFPLIEREKVTVTSLVPAVLRLWADSGRRHHDLSRLVLQIGSAPLDSALATR
                     ARKVLECGLTRWFGTTEGFLAHTRLDDPEEVIENTEGRPLSEADEMLVVGPSGEPLPDG
                     EAGELLVRGPYTIRGYYRAPEQNARSFTPDGFYRTGDLACRRPDGNLVILGRIKDVINR
                     "
     CDS_motif       7188..7277
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50825_0001"
                     /evalue="7.30E-08"
                     /label="NRPS-A_a6"
                     /locus_tag="IE289_RS50825"
                     /protein_end="397"
                     /protein_start="367"
                     /score="24.8"
                     /tool="antismash"
                     /translation="PDGEAGELLVRGPYTIRGYYRAPEQNARSF"
     gene            7712..8377
                     /locus_tag="IE289_RS50830"
                     /old_locus_tag="GCM10010317_104740"
     CDS             7712..8377
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) KtzT"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016641415.1"
                     /locus_tag="IE289_RS50830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_104740"
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="WP_190174897.1"
                     /sec_met_domain="KtzT (E-value: 6.4e-94, bitscore: 301.6,
                     seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MFVPATYRQPDQSWTIDMIRSNPLALLVTADPLGPGVHATHVPVI
                     LDPAAGDPTAGDPAEIVLLGHMNRANPHWAAVEAGEALVVFTGPDSYVSPTVYQTTPAA
                     PTWDFTSVHVRGPVQLLDEGEPTLAVVQATVRAYEAEFGTGWDMSESVGYFRKILPAVG
                     AFRIHGRRTEAMFKLSQEQPPEVRERVRESFGGDPTGRRGEVADLMCRWRREPGPAAR"
     gene            complement(8386..9645)
                     /locus_tag="IE289_RS50835"
                     /old_locus_tag="GCM10010317_104750"
     CDS             complement(8386..9645)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Orn_monoox"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1080: lysine/ornithine N-monooxygenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016641416.1"
                     /locus_tag="IE289_RS50835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_104750"
                     /product="lysine N(6)-hydroxylase/L-ornithine
                     N(5)-oxygenase family protein"
                     /protein_id="WP_190174898.1"
                     /sec_met_domain="Orn_monoox (E-value: 1.2e-148, bitscore:
                     483.6, seeds: 18, tool: rule-based-clusters)"
                     /sec_met_domain="Pyr_redox_2 (E-value: 5.4e-10, bitscore:
                     27.3, seeds: 64, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPENVVYDVVGVGFGPANLSLAVALTEHADDLTYLFLDRAPSMSW
                     HGGMLLPGAKMQVAFLKDLATFRNPVSRFSFVAYLHDTGRLAQFVNNRDFFTTRRDFHA
                     YLDWAEAGFADQVRRGVTVTGIEPTGNGDSMRVMTEGSPGTDSIEARNVVISTGLVPKM
                     PPGIAEDQYVWHSSRFLDNYRAVRGPLRRVGVAGAGQSAAELVRYLYDDLPDAEIFAFV
                     PSYGYSVADSTPFANRVFDPQGMDDFYSAPAQTKSAIWQYHKNTNYSVVDDGVIRDLYQ
                     RAYDEETSGRNRLHFHTMARVESAKRMADDTRVTVYSTLSDSSYELDLDVLICATGYDP
                     MDPAKMLGGMDGCLARDEEGRYRVRRDHRLVTASDPACGVYLQGGTEHTHGLSASLLSN
                     IAIRSGEIADSIAAAKRARR"
     gene            9934..>12348
                     /locus_tag="IE289_RS50840"
     CDS             9934..>12348
                     /NRPS_PKS="Domain: Condensation_Starter (2-309). E-value:
                     3.4e-60. Score: 195.8. Matches aSDomain:
                     nrpspksdomains_IE289_RS50840_Condensation_Starter.1"
                     /NRPS_PKS="Domain: AMP-binding (472-805). E-value: 2.1e-85.
                     Score: 278.7. Matches aSDomain:
                     nrpspksdomains_IE289_RS50840_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IE289_RS50840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="AMP-binding protein"
                     /protein_id="WP_190174899.1"
                     /sec_met_domain="Condensation (E-value: 9.2e-33, bitscore:
                     101.9, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.6e-81, bitscore:
                     262.3, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPSLALSAAQERMWFAHAISPEVPNNGLALWQIDGEVSRTELISA
                     IRRTVAEARTLLVNFREEDDGLRQVFRELGDWQPFSIDVSGHADPHTAAGEIVSDLVGR
                     PFDFGSDLLLRAGVIVLADDRLQLVVVCPHLISDGFGILNLLAGRIADIYTALRKDLPI
                     PPSTFAAPEAFHRADRRYRDSARAAADAAFWRDYTADAGEVVRLPKSVSKTDVTELEPP
                     RLSTAARLGMCEFEACVPAAEYAKWQEVAASLGVAMTSLTTAAVAVFFRRMCRQSEPLF
                     SLSVNNRFGETRATPGMVQNVLPIRIAFRPGISLAELAEVVTTETRSIYRHSSLQISSI
                     RRDAGLSHEPRSPFGAILNLIPFLEPVDFDGSPARLSGSSFGVTDELMISVYVDGTSAT
                     GDLCVRFDAPGALYDRDDLAALCSRLIECLRSVMADPHAPVDVAQTLSPAELSWLAARE
                     DTTAPVPDATLVSLFAEQAGATPDATALVCDGVTVSYAELDQRSSGLAQALAARGAGEE
                     RFVAVALPRSIDLVVSVLAVLKTGAAYVPVDPGYPSDRIALMLDDADPVLVITRPDVDL
                     PATGHPRISPDERGGGVAVPPRHGDQPAYLVYTSGSTGAPKGVVVPHANVTRLFAATES
                     LFRFRPDDVWTLFHSPSFDFSVWEIWGPLLHGGRLVVVSQQTSRSPREFRRLLAAEGVT
                     VLNQTPSAFQQLAETDADFPDDLALRLVVFGGEALDPRTLSGWYERHGDRAPLLVNMYG
                     ITETTVHVTYRPLDTTTVGSVIGTPVPNMHARVLDSGLCLVPVGVVGELYVS"
     aSDomain        9940..10860
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE289_RS50840_Condensation_Start
                     er.1"
                     /domain_subtypes="Condensation_Starter"
                     /evalue="3.40E-60"
                     /label="IE289_RS50840_Condensation_Starter.1"
                     /locus_tag="IE289_RS50840"
                     /protein_end="309"
                     /protein_start="2"
                     /score="195.8"
                     /specificity="C activity: active"
                     /specificity="Active site motif: PHLISDG"
                     /tool="antismash"
                     /translation="SLALSAAQERMWFAHAISPEVPNNGLALWQIDGEVSRTELISAIR
                     RTVAEARTLLVNFREEDDGLRQVFRELGDWQPFSIDVSGHADPHTAAGEIVSDLVGRPF
                     DFGSDLLLRAGVIVLADDRLQLVVVCPHLISDGFGILNLLAGRIADIYTALRKDLPIPP
                     STFAAPEAFHRADRRYRDSARAAADAAFWRDYTADAGEVVRLPKSVSKTDVTELEPPRL
                     STAARLGMCEFEACVPAAEYAKWQEVAASLGVAMTSLTTAAVAVFFRRMCRQSEPLFSL
                     SVNNRFGETRATPGMVQNVLPIRIAF"
     aSModule        9940..>12348
                     /domains="nrpspksdomains_IE289_RS50840_Condensation_Starter
                     .1"
                     /domains="nrpspksdomains_IE289_RS50840_AMP-binding.1"
                     /incomplete
                     /locus_tags="IE289_RS50840"
                     /starter_module
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       10027..10119
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50840_0001"
                     /evalue="1.50E-02"
                     /label="C2_starter_024-063"
                     /locus_tag="IE289_RS50840"
                     /protein_end="62"
                     /protein_start="31"
                     /score="8.1"
                     /tool="antismash"
                     /translation="QIDGEVSRTELISAIRRTVAEARTLLVNFRE"
     CDS_motif       10330..10395
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50840_0002"
                     /evalue="3.30E-06"
                     /label="C3_starter_134-155"
                     /locus_tag="IE289_RS50840"
                     /protein_end="154"
                     /protein_start="132"
                     /score="20.0"
                     /tool="antismash"
                     /translation="PHLISDGFGILNLLAGRIADIY"
     CDS_motif       10903..11010
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50840_0003"
                     /evalue="1.00E-05"
                     /label="C67_starter_14fromHMM"
                     /locus_tag="IE289_RS50840"
                     /protein_end="359"
                     /protein_start="323"
                     /score="18.1"
                     /tool="antismash"
                     /translation="TETRSIYRHSSLQISSIRRDAGLSHEPRSPFGAILN"
     aSDomain        11350..>12348
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE289_RS50840_AMP-binding.1"
                     /evalue="2.10E-85"
                     /label="IE289_RS50840_AMP-binding.1"
                     /locus_tag="IE289_RS50840"
                     /protein_end="805"
                     /protein_start="472"
                     /score="278.7"
                     /specificity="substrate consensus: Thr"
                     /tool="antismash"
                     /translation="FAEQAGATPDATALVCDGVTVSYAELDQRSSGLAQALAARGAGEE
                     RFVAVALPRSIDLVVSVLAVLKTGAAYVPVDPGYPSDRIALMLDDADPVLVITRPDVDL
                     PATGHPRISPDERGGGVAVPPRHGDQPAYLVYTSGSTGAPKGVVVPHANVTRLFAATES
                     LFRFRPDDVWTLFHSPSFDFSVWEIWGPLLHGGRLVVVSQQTSRSPREFRRLLAAEGVT
                     VLNQTPSAFQQLAETDADFPDDLALRLVVFGGEALDPRTLSGWYERHGDRAPLLVNMYG
                     ITETTVHVTYRPLDTTTVGSVIGTPVPNMHARVLDSGLCLVPVGVVGELYVS"
     CDS_motif       11536..11577
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50840_0004"
                     /evalue="1.10E-05"
                     /label="NRPS-A_a2"
                     /locus_tag="IE289_RS50840"
                     /protein_end="548"
                     /protein_start="534"
                     /score="18.5"
                     /tool="antismash"
                     /translation="LAVLKTGAAYVPVD"
     CDS_motif       11743..11802
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50840_0005"
                     /evalue="1.30E-09"
                     /label="NRPS-A_a3"
                     /locus_tag="IE289_RS50840"
                     /protein_end="623"
                     /protein_start="603"
                     /score="30.0"
                     /tool="antismash"
                     /translation="AYLVYTSGSTGAPKGVVVPH"
     CDS_motif       12289..12321
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS50840_0006"
                     /evalue="7.20E+01"
                     /label="NRPS-A_a2"
                     /locus_tag="IE289_RS50840"
                     /protein_end="796"
                     /protein_start="785"
                     /score="-1.9"
                     /tool="antismash"
                     /translation="VLDSGLCLVPV"
ORIGIN
        1 ccggcccggc cggcccggcc ggccccgccg gcccggccgg ccccgccgcc cgtggcgacg
       61 ggacgccggc gtccatggcc tcgcccggtt ccggaagccc cactccgccg agcaggctgt
      121 gcagcagcac ttcgtcgtcg ccgagggcga agagcgtccg taacggcttc tcgtcccgca
      181 gcagtccgat ctggcacaac ccgagcgggc cctcgatggc cgactcggcc agcagctgac
      241 tcatcgcgcc cgcctcgatg aggcagaagt cgtgggcgcg ccgcccgtac atcggctcga
      301 cctcggccat cgggccgacg aggagcaggt ggaacgcggc cgaggagaag gcgtcctggt
      361 tcatcgagag gtgtatggac gccggcatct cgacaccact gcgtacggtg cgcaaccggt
      421 gtgtcagtgg gtcgtagtag taggagccgc cgggcagccc gccaactcgc ccggggtgga
      481 cgtagacgta ggtccgcacc gggtacagcg atccggccga accgtaccgg tacttcggca
      541 ccgggcggtg tgcggcgccg acggcccgca ggcagttgag cagccgtgcg agttcggcca
      601 acggaacctc ggcctccgcg aaccgccgat ggctgcgccg ccggttccac cggtggccgg
      661 tccgctccgg gtcgaggaag tcgggcagtc cgaccgccgg cgaggtcagg tcggcacgcc
      721 ggccacgctg ggccgcggtg acggccgcgc ggtgggcgga atccgtgatc acggcgccga
      781 gcaggctctc gtactcggcg ccgtcgccac cggcttccgg ggccggggcc ggccgtcgcg
      841 cacggtccgg tacgacgaac gtggtgaggt acgggtggtg gcgggtcccg gtggcagtgg
      901 tgaccgccct cgcgatcgcc gggtgctggg cgagcgcggc gtcgatctcg ccgagctcga
      961 tgcggaagcc accgatcttg acctggccgt ccgtccgtcc caggaactcg atcgtgccgt
     1021 ccggccgcca acggcccaga tctccggtgc gatacagccg ctctccggac accgggtggg
     1081 tgatgaaccg ctccgcggtg cgcaccgggt caccccagta cccctcggcc agccccaggc
     1141 cgccgatgta cagctcgccc accacccaga ccgggcagtg gtccaaccgg tcgtcgagga
     1201 cgtggaagct ctgattcgtc agtggccggc cgtacgggat ggagtcccat tcggggtcga
     1261 cctcgccgat ttcgtaagag atggaccaga tggcggcctc ggtggccccg cccagcgaga
     1321 tcagccggca cgcgggcgac cactcgcgcc agcggtcggg caggccgacg gggatccagt
     1381 cgccggacca caacgccacc cggataccgg ccaacgcagc gccctgccgc ccaccggcac
     1441 cgtgtccgtc accggtgccg tctccggcac cggcaccggc accggcaccg gcaccggcac
     1501 cggcacccaa gtgagagacg accatttccg cgaccgcggg cacgctgttc cacaccgtca
     1561 ccccgtgctc ggccaccaac tccagccagt gttccggatc ccgctgcgaa ccgggccgcg
     1621 gcaacaccag ccggccgccc gcccccagca ccccgaacac atcgaacacg ctcaggtcga
     1681 aactcagcga cgacagcccc agcaccgcgt cgtcggcccc gatcccgaac cggtcgcaga
     1741 cgtctgtaca ggtgcccagc gccgcctgat gcgagaccgt cacgcccttg ggcgcaccgg
     1801 tggagccgga ggtgtagagc acgtaagcca catccgtggg cttcgccgac gattcggcga
     1861 gccgtcgggc ccggtcccgg cagtcctcca cggcgacgac ccgaaccgtg tcgggccact
     1921 gctggtggtc gtcgccggtg cggacgagga ccaccgaaca gcctgcggcc gcgagcagtt
     1981 cggcgcgccg ccgcggaggc agatccacat ccaccggcag ataggcccgg ccggccaaat
     2041 ggacgcccag ggcagccacc acttgctccg ccgacttcga catccccacc gcgatcaagg
     2101 cgcccgggtc ggcgtccggg cccaggaggg tttccgcgac cccggacgcc cacaacccca
     2161 gttccccgta ccgcagcacg ccgccgtcgg ccgtcaccac cgccacccgg tccggctcgc
     2221 gcgctgccca gcccatcacc ccgtcggcca acgacgccga cggcaccgcg ccgtccgtcg
     2281 cgtccacccg ggccacgaac tcgtccgggc cggcaccgca cacgtccacg ccgcccgacc
     2341 acgccgcggc gtcgaccagg ctgtcgagca agcggcagta ggcgtcgaac atctcgtccc
     2401 gcacgccggg caggaacgcg tcgaccggcg agtcccagtt gaactccagc gccccggcgt
     2461 tctcgtacac ctggtggtcc agcaggacct gaggggtctg cgaaatgctc agcgtggtct
     2521 caccgagcca gttctggccc aacgtcccgg cgtcccggcc gtgctggtcc cagccctggt
     2581 cgcgggcgct ggcgaacacc accgggcagt gcgggacgcc cgtggccggg cgcgccttgg
     2641 ccagctcgcg ctgcaccgcc accccgtcga acgcgcggtg ttccaagtcg cgccagagct
     2701 gccggtgcag ccggtcggcc agttcggcga tgccggggcg gccacggagg tccacttcga
     2761 gcaggaccac gctggtgaag tcgccgatca gggcgtcgac gtccggatgc acccggcgcc
     2821 ggctgaacag cggcaggttc agcaggaacc ggtcggtcgt ggaccagcgc ccgagcaccg
     2881 tggcgaaggt cgcgagcaac accgacgtcg gggtgagctc gtgctggagc gccagttgtc
     2941 tgatgcgtga ccaggaccgg gccggcagcc tgtgagccct tctgctccac cgtgcgggcg
     3001 cggtcgcgcc acgccgccac ggcagttgcg gcccctcggg gagttcgggc acccgtcgca
     3061 gccagtagtc gcgggcggcg gcgtattccg gatcttccgg atcgacggcg tgggcgttga
     3121 cgcagtcgcg aaaggtggtc cgcagcggtg gcagccgctc gccggcgtag agccgccgcc
     3181 agtcgtcgaa gaacagcctg atgctggcca cgtcggcgat cagcaggtcg atactgatgg
     3241 ccagacggat acggtcgccc ggcaggatca gcgcccgcac ttcccacatc ggccactgac
     3301 cgatcggcct gatctcgtgg gacagttcct cacgtatggc ggcggcggtc cgcggcccgg
     3361 actccgtgga gcagtcggtg accgagaacg ggaccggcgt ggcgtccgat tccgggagca
     3421 cccgctgggt gccgtcctcc gccaccaccg cgcgcaacat gtcatgccgg gagaccagta
     3481 cggtcagcgc cgactccaac cggcgcagat ccaggtcgcc cgcctcgatc tccaggtaca
     3541 ggtgggtcgc gacatcgccc cgttcctcgc cggcgcgcct gccggcgagg tatgcctgct
     3601 ggagaccggt cagccgaaag ggctcgagcc ggtgctccgg ggcggcgtgg aacggctcgg
     3661 acggctcgaa cggctcgaac ggctcggatg gctcggatgg ctcgaacggc tcggacgccg
     3721 ccgacgcgtg ccgaccgtcg agccggtgga ccatcagctc ggtgagttca tccacggtcg
     3781 agcgcaagaa ggtggccagc ggaagatcca cctgaacgtc ccgttccagc acggcacgca
     3841 gttccatggc ccgtatcgat tcgagcccca gagcgaccag cggcacggcg gtgggaacgt
     3901 cgtcggtggg aacgccgagc agatcgccga gacgccgccg caggtcagcc gatatcacac
     3961 cagcccgcag ggacgggtcc agggccgcga gcagaccggg caggacggca ctcccgccgg
     4021 tggaccgacc gctcaccggc cgaaccggtg ccgacgcgga cggcgggccg gcatggagtg
     4081 gttcgagcag tgtttcgagc tcgcccgcga gatagcggcg tcggcactcg ccgcgttgga
     4141 tcttgccgct gatcgtcttg gggatctggt cgggcgacac cagcaccagg tggtgcacgt
     4201 ccacctcgtg cgccgcggtc accgcggagc ggatacgccg ggccagctcc ggcaacggga
     4261 cggggagcgc gtccgcgtcc ggcactgact ggacaagcac cagctcctcg ccggcgtcgc
     4321 ggttcacgga gaacgccgcc gccggcgctc cgcgcagcgc gggatgcgcc cgctcactgt
     4381 ccgactcgat gtcgtggggg tagaggttgc gcccccgcac gatgatcatc tccttgcgcc
     4441 ggccacacag caccagttca ccgttgtgca ggaaggccat gtcgccggaa cggagataag
     4501 ggccctcgcc gtcggacagc cgcgcgccga acatctcctc ggtcagttcc gcgcgcttcc
     4561 aatagccctg gcacaccacg gggccgcgga tccacacttc gccgacctcg tgctcgccga
     4621 gccgccgcct gctctccggg tccaccacga tcacttcgga gtacggaccc ggctggccga
     4681 ccccgaccag acgcagcctg cgttcgccct cggcggcgcc tccgtccggg accgcgtccg
     4741 gctcgccgag taccaccgct ctgccctgtt ccagctcggc cgcgtccacg tccagcacga
     4801 gcggcctgcc ccggcgctgc ccggtcagca ccatggcctc ggtcatgccg aagccggggc
     4861 acatggcggc cgtccggaag ccgtgcggct ggtatgcctc gatgaaccgg gccatggtgt
     4921 gcgcgtggac gcgctcggcg gtgttgagcg tggtgcgcca gccggacagg tcgagccctg
     4981 tgcgctcccg ctcgctgatc tgatcggcgc agaggccgaa ggcgaagttc ggcgcgcagg
     5041 acagcacggg ccccggcgtg tcggagatgg cgcgcaacca gcgcagcggg tgccctacga
     5101 agtcctccgg caccaggaac accgcctggc cgcctaggta ctgcgacagc atgaggtgca
     5161 cgacgattcc gagcgcgtgg tagggcgata tccagctgac cacgttgctg ccgggcggga
     5221 gctggaagat cgccgccgcc tgcggcagtt ggtgcaccag gttgtcgtgg ctgatcatca
     5281 cgcccttcgg ccctccggtg gccccggagg tgtattgcag caaggcaaga tcgccccggt
     5341 cggtggcggg gacggcgaga tgcccgctcg ccgcgggttc cacgtcgtcc accgcgatcc
     5401 acggcacgtc gccgaaccgg cggcgcagct cgccgaaccg gtcccggatc tcacgggtcg
     5461 tcaccaccgc cgagacctgc gcgtcggacg cgatccggac cgcccgctcg gtgcgcggat
     5521 cgctctcgtc gctcacgggt ggcgggcagg tcacggcgac cgcgccggcg tagagcgtgc
     5581 cgaacagacc gacaatgaag tccaggcccg gcggcgcgag tatcagcacg cggtgtcgcg
     5641 cgagctcgtg cgcggccagc gcggcggcca gcgcgcagca ccgggtgtcc agttcggcac
     5701 ggctgagcga gacgcgccgc ccgtgctcgg ggccgtcggc cgggaacgtc aggtggctga
     5761 aggcgaccgc gtccggatcg cgaaccgcgt tggcccgcaa catgtcgacg acggtgccgc
     5821 tgtggaccgc agcggtgaca tcggtcactg gacgctcctg tcgatggtcg gcccagcgtc
     5881 cgccggacgc ggccacagtg gtccgtgcca gaaggcgagc caggggtcgg agtgtcttca
     5941 ccgtgccagt cggccgtcgc cccctgtccg ccgaggaaag atcttggcaa gacatcagcc
     6001 cgcgcgcggt acagcgcggc gacgatgcgg gtgaggaggg tccggggcaa gaccgccgac
     6061 cggtcccggc gacgagggaa gtgatcatga tcgatggctt cgtcccgtgg cccgacggac
     6121 tcgcccagga gtaccgccgg gacggctact ggctcggtcg cccactcggc gatctcttcc
     6181 aggagtcctg tgcgcggcac gccgatcgcg tggccgtcgt ctgcggtgaa cgcgaactga
     6241 cctacgccga gctgggggac agcgcgcaac ggctggcgta cgggctgctg gagctgggcg
     6301 tcaggccgtc ggaccgggtc gtggtgcaga tgccgaacat cccggagttc gtcgtgctgc
     6361 tcctggcgct gctgcgcatc ggggccattc ccgtcctggc cctgcccggg taccggcgcg
     6421 tcgagatggc gcatgtcggc gcgcacagcg gcgccgtcgc gtacgcggtc aaggacgagt
     6481 tcggcggctt cgacttccgg cggctcgccc gtgaggtctc cttcgcgggg catgtgctcg
     6541 tcagcggcga cgcccaggaa ttcgtcgctc tcgacgcgct cgcgggtgcc ccgcgggagc
     6601 tgccgtcggt caatccgtcc gacccggcgc tgttcctgct gtccagcggc acgacggggc
     6661 tgcccaagct gatcccacgc acccatgacg aactcgctta cctcatgcgg gccaccgccc
     6721 gggcgatggg ggcgggctcg gacctggtct acctcgcggt gaacccggtg tcgcacacgg
     6781 cggccctcgc ctgtccgggg gtgctgggga gcctgctgct cggcggcaag gccgtgctca
     6841 cctcgagtgt gcggcccgac gaggtgtttc cgctgatcga gcgggagaag gtcaccgtca
     6901 cctcgctcgt gcccgcggtg ctgcggctgt gggccgactc cggccggcgg caccacgacc
     6961 tctcccggct ggtgctccag atcggcagcg ccccgctcga ctccgccctg gcgacacggg
     7021 cccgcaaggt gctggagtgc gggctgaccc gctggttcgg cacgaccgag gggttcctcg
     7081 cccacaccag gctggacgac ccggaggagg tgatcgagaa caccgagggc cggccgctct
     7141 ccgaggccga cgagatgctg gtggtggggc cgtccggtga gccactgccc gacggggagg
     7201 ccggcgagct gcttgtgcgc ggtccgtaca ccatccgcgg gtactaccgg gcgccggagc
     7261 agaacgcgcg atcattcact cccgacgggt tctaccgcac cggcgacctg gcctgtcgac
     7321 gtcctgacgg aaacctcgtg atcctcggaa ggatcaagga cgtcatcaac cgggcgggcg
     7381 agaaggtctc cccggaggaa gtggagcggc atctgtgcgc gcatcccgcg gtgcgggacg
     7441 cggccgtggt cggcgtcccg gaccgcacct tgggtgagcg gacatacgcc tttctcctgc
     7501 tgcggaccga cgtcacgtca gccgccgtca agacccacct gcgcgagcgc ggcctcgcca
     7561 cgtacaagat ccctgaccgg atcgtcacgc tggccgacct gccgcggacc gcgatgggca
     7621 agaccgacaa ggaagcgctg cgcgcccggg cgcgcagctc gtgaggcgtg ccgacacccg
     7681 tcgaacgtcg agagacccgg aaggggtgga cgtgttcgtc cccgcaacct accgtcagcc
     7741 ggatcagagc tggaccatcg acatgatcag gtcgaacccg ctggcgctgc tggtcaccgc
     7801 cgaccccctg gggccgggtg tgcacgccac ccacgtaccg gtcatcctcg acccggcggc
     7861 gggcgatccg acagccggcg atccggcgga gatcgtcctg ctcggccaca tgaaccgcgc
     7921 gaacccgcat tgggcggcgg tggaagcggg cgaggcgctg gtggtgttca ccgggccgga
     7981 ttcgtacgtc tcccccacgg tctaccagac cacgcccgca gcgccgacct gggacttcac
     8041 ctccgtgcac gttcgcggcc cggtgcagct actcgacgag ggcgagccca cgctggccgt
     8101 cgtgcaggcg accgtgcggg cctacgaggc cgagttcggc acgggctggg acatgtccga
     8161 gtcggtgggc tacttccgca agatcctgcc cgcggtcggg gccttccgca tccacggccg
     8221 acgcacggag gcgatgttca aactcagcca ggagcaacca cccgaggtca gggagcgcgt
     8281 ccgggagtcc ttcggcggcg acccgaccgg ccgccgcggc gaggtcgccg acctgatgtg
     8341 ccgctggcgg cgggaaccgg ggccggccgc gcggtgacgg actcctcagc gccgcgctcg
     8401 tttggccgcc gcgatcgagt cggcgatctc gccgctgcgg atcgcgatgt tcgacagcag
     8461 cgacgcactg aggccgtgtg tgtgctcagt gccgccttgc aggtagacgc cgcaggccgg
     8521 atcggaggcg gtgaccagcc ggtggtcgcg gcggacgcgg taccggccct cctcgtcgcg
     8581 ggcgaggcac ccgtccatcc cgccgagcat tttcgcaggg tccatggggt cgtagccggt
     8641 ggcacagatc aggacgtcca ggtcgagttc gtaggagctg tccgacagcg tcgagtaaac
     8701 ggtgacgcgg gtgtcgtctg ccatgcgctt cgcgctttcc accctcgcca tcgtgtggaa
     8761 gtgcaggcgg ttgcggccgg acgtctcctc gtcataggcg cgctggtaca gatcccggat
     8821 gacgccatcg tcgaccaccg agtagttcgt gttcttgtgg tactgccaga tggccgactt
     8881 ggtctgagcc ggtgcggagt agaagtcgtc catcccctgc gggtcgaaca cccggttggc
     8941 gaacggcgtg ctgtccgcca cggagtaccc gtacgacggc acgaacgcga agatctccgc
     9001 atcgggcagg tcgtcgtaga ggtaccgcac gagctccgcc gcgctctgcc cggcaccggc
     9061 cacacccacg cggcgcagcg gtccgcgcac cgcgcggtag ttgtccagga atcgcgagct
     9121 gtgccagacg tactggtcct ccgcgatgcc gggcggcatc ttcggcacca gcccggtcga
     9181 gatgacgacg ttgcgcgcct ctatcgagtc ggtaccgggc gacccctctg tcattacccg
     9241 catgctgtcg ccgttcccgg tcggctcgat gccggtcacg gtcactccgc gccgcacctg
     9301 gtcggcgaaa ccggcttcgg cccagtcgag gtaggcgtgg aaatcacggc gggtggtgaa
     9361 gaagtcccgg ttgttgacga actgcgccag ccgtccggtg tcatgcaggt aggccacgaa
     9421 gctgaaccgg gagaccggat tgcggaatgt ggcgaggtcc ttgaggaacg cgacctgcat
     9481 cttcgcgccc ggcaacagca ttccgccatg ccagctcatc gacggcgccc ggtcgaggaa
     9541 aaggtacgtc agatcatcgg cgtgctcggt cagcgcgacg gcgagcgaca ggttggccgg
     9601 cccgaaaccg acgccgacga cgtcatacac cacgttctct ggcacccgtc ctcctcgtta
     9661 ccgaattcct gcgggggagc ctacgcaccc ggcccgttac tggaacgcgg gcggaaagaa
     9721 gcaggaaaga gtgatcctgt gaccggccgg tatgtggtga actcccgcta tggggacgcg
     9781 aatcccgtac cgccgctcgg catctcgccc gaaaccctgt tgcgaccacc caccgagcgt
     9841 cgacgaaaag aggcggctgg cctcggcgtc ctcagccggc acgtgatgtc gtgctgtctg
     9901 acgctccatg agcacacatc gaggtgaatg gaaatgccgt cgctagccct ctcggccgcg
     9961 caggaacgga tgtggttcgc tcacgccata tcgcccgagg tgccgaacaa cggtctggct
    10021 ttgtggcaga tcgacggcga ggtgtcccgc acggagctca tctcggcgat tcgcaggacc
    10081 gtggcggaag cgcgcacgct gttggtcaac ttccgcgaag aggacgacgg cctccggcaa
    10141 gtgttccggg aactcggcga ctggcagccg ttctcgatcg acgtctccgg acacgccgat
    10201 ccgcacaccg ccgcgggcga gatcgtgtcg gatctggtgg gacgaccgtt cgatttcggg
    10261 tcggacctgc tgctgcgcgc gggtgtgatc gtcctcgccg acgaccggct gcaactggtg
    10321 gtggtgtgcc cgcacctgat ctccgacggc ttcgggatct tgaacctcct ggccgggcgg
    10381 atcgccgaca tttacacagc actgcgcaag gacctgccga tcccgccgag taccttcgcc
    10441 gcacccgaag ccttccaccg ggccgatcgg cggtatcgcg actcggcccg cgccgcggcc
    10501 gatgcggcgt tctggcgcga ttacacagcg gatgccggcg aagtcgtccg gctgccgaaa
    10561 agcgtctcga agaccgacgt caccgaactg gaaccgcccc ggctctcgac agccgcgcgg
    10621 ctcgggatgt gcgaattcga agcatgcgtt ccggccgcgg aatacgcgaa atggcaagag
    10681 gtcgcagcgt ccctgggcgt cgccatgaca tcgctgacca ccgccgcggt cgcggtgttc
    10741 ttccggcgga tgtgccggca gtccgaacca ctgttctcgc tcagcgtgaa caaccggttc
    10801 ggcgagacga gggcgacccc ggggatggtc cagaacgtcc ttccgatacg gatcgcgttc
    10861 cggcccggca tctcgctggc ggagctggcc gaggtggtca ccaccgaaac ccggagtatc
    10921 taccgccact cgtcgctaca gatatccagc atccggcgtg acgccggctt gagccacgaa
    10981 ccgcgcagtc cgttcggcgc gattctcaac ctcatcccgt tccttgaacc ggtcgacttc
    11041 gacggcagcc cagcacgcct gtccggcagc tcgttcggtg tcaccgacga gctgatgatc
    11101 tccgtctatg tcgacggcac cagtgccacg ggcgacctgt gtgtccggtt cgacgccccg
    11161 ggagcgctct acgaccggga cgacctcgcc gcgctgtgtt cccggctgat cgaatgcctg
    11221 cggtcggtga tggcagaccc gcacgcgccg gtggacgtgg cacagaccct gagcccggcg
    11281 gaactgtcct ggctggccgc gcgcgaggac acgacggcac cggtccctga cgccaccctc
    11341 gtgtcgttgt tcgccgagca ggccggtgcg acgcccgatg cgacggcgct ggtgtgcgac
    11401 ggcgtcaccg tctcgtacgc ggaactggac cagcgctcaa gcggcctcgc acaggcgctg
    11461 gccgcaaggg gtgcgggcga ggagcggttc gtggccgtgg cgctgccacg gtcgatcgac
    11521 ctcgtggtct cggtgctggc ggtgctgaag acgggggcag cgtacgtacc cgtagacccg
    11581 ggctatccgt cggaccggat cgcactcatg ctcgacgacg ccgacccggt cctggtgatc
    11641 acacgcccgg acgtcgacct gcccgcgacc gggcaccccc ggatatcgcc cgatgaacgc
    11701 ggcggagggg tcgcggtccc gccccggcac ggcgaccagc ccgcatatct cgtctacacc
    11761 tcggggtcaa ccggggctcc caaaggcgta gtcgttccgc acgcgaacgt gacgcgcctg
    11821 ttcgccgcca ccgaatcgtt gttccgcttc cgtccggatg acgtctggac gttgttccac
    11881 tcgccgtcct tcgacttctc cgtctgggag atctggggcc cgctgctgca cggcgggcgg
    11941 ctcgtcgtcg tgtcccagca gaccagccgc tccccccggg aattccggcg gctgctggcc
    12001 gccgaaggcg tgaccgtgct caaccagaca ccgtcggcgt tccagcagct ggccgagacg
    12061 gacgcggatt tcccggacga tctcgcgctg cgactcgtgg tcttcggtgg ggaggcgctc
    12121 gatccgcgga cgctcagcgg gtggtacgaa cgccacggcg accgggcacc gctgctggtg
    12181 aacatgtacg ggatcaccga gacgaccgtg cacgtcacct accggccgct cgacacgacc
    12241 acggtaggca gcgtgatcgg cactccggtc ccgaacatgc acgcgcgtgt gctggactcc
    12301 ggtttgtgtc tggttccggt cggtgtggtc ggtgagttgt atgtgtcc
//
