LOCUS       NZ_BMUQ01000032         7754 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces mirabilis strain JCM 4551 sequence32, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUQ01000032
VERSION     NZ_BMUQ01000032.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces mirabilis
  ORGANISM  Streptomyces mirabilis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 100661)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 117X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650275.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:52:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,392
            CDSs (total)                      :: 10,311
            Genes (coding)                    :: 9,898
            CDSs (with protein)               :: 9,898
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 413
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 172 of 414
            Pseudo Genes (incomplete)         :: 329 of 414
            Pseudo Genes (internal stop)      :: 40 of 414
            Pseudo Genes (multiple problems)  :: 115 of 414
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:21:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 7754
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUQ01000032.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..7754
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..7754
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..7754
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[1647:2754](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(1648..2754)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(<1..177)
                     /locus_tag="IE289_RS45295"
     CDS             complement(<1..177)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018510407.1"
                     /locus_tag="IE289_RS45295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="WP_308435128.1"
                     /transl_table=11
                     /translation="MRKVLIANRGEIAVRVARACRDAGIASVAVYAEPDRDASHVRAAD
                     EAFALGGDTPATSY"
     misc_feature    complement(136..147)
                     /note="TFBS match to CcpA, Catabolite control protein A,
                     confidence: weak, score: 15.68"
                     /tool="antismash"
     misc_feature    complement(223..239)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.06"
                     /tool="antismash"
     misc_feature    complement(543..558)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.53"
                     /tool="antismash"
     misc_feature    604..617
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 18.47"
                     /tool="antismash"
     gene            complement(614..1591)
                     /locus_tag="IE289_RS45300"
                     /old_locus_tag="GCM10010317_093280"
     CDS             complement(614..1591)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (5-196). E-value: 2.3e-11.
                     Score: 35.2. Matches aSDomain:
                     nrpspksdomains_IE289_RS45300_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546906.1"
                     /locus_tag="IE289_RS45300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_093280"
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_190174079.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 4e-12, bitscore:
                     38.3, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPTILITGAAGFVGGHVTREAARHRADLRLMSHRSPLLGSRQPTQ
                     EGPPPHEGPAAHDGARVVRADLTDPASLRGVCDGVDVLIHCASQIGGGVEANEAVNARG
                     TAALIEEARRAGVARIVQLSTASVYGRGTFRGDRPEELRRNPGSPTSRTRAAAEDTVLA
                     AGGVVLRPHLVYGEGDRWVVPGLTRMLRVLPGTVEGWPARSSAIAVPELATLLVATALA
                     PAADLTASVYHATHPEPLPVDTLLRTVAECTGADWPDRELTVDRARAVLAENGVPPSGL
                     DMFTTDHVFDSTPLWSDLRRAPGAGFDTDFPLAAPWYRKALRDG"
     aSDomain        complement(1004..1576)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE289_RS45300_NAD_binding_4.1"
                     /evalue="2.30E-11"
                     /label="IE289_RS45300_NAD_binding_4.1"
                     /locus_tag="IE289_RS45300"
                     /protein_end="196"
                     /protein_start="5"
                     /score="35.2"
                     /tool="antismash"
                     /translation="ITGAAGFVGGHVTREAARHRADLRLMSHRSPLLGSRQPTQEGPPP
                     HEGPAAHDGARVVRADLTDPASLRGVCDGVDVLIHCASQIGGGVEANEAVNARGTAALI
                     EEARRAGVARIVQLSTASVYGRGTFRGDRPEELRRNPGSPTSRTRAAAEDTVLAAGGVV
                     LRPHLVYGEGDRWVVPGLTRMLRVLPGT"
     CDS_motif       complement(1514..1585)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS45300_0001"
                     /evalue="2.90E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE289_RS45300"
                     /protein_end="26"
                     /protein_start="2"
                     /score="10.3"
                     /tool="antismash"
                     /translation="TILITGAAGFVGGHVTREAARHRA"
     gene            complement(1648..2754)
                     /locus_tag="IE289_RS45305"
                     /old_locus_tag="GCM10010317_093290"
     CDS             complement(1648..2754)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014627801.1"
                     /locus_tag="IE289_RS45305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_093290"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_190174080.1"
                     /sec_met_domain="AfsA (E-value: 1.2e-23, bitscore: 75.6,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYTTSTRVSVEDDVATVGTSASRTASRPAARNAVQPAVQTSAQT
                     SAQTSAQASVQTAARTLTPRLTTTVPREYVHRAAVSEVLLTGWEAAAEPAGPDPDEFAV
                     SAQWPRSHSFFTQSGGYQDPMLLIESVRQIGSLLAHAEFGVPFGHQFLMWDMFFSTSPE
                     LLVADAVPTEVELRTVCRDIVRRGRVLGGMRYDVTVLRDGRALATAGAAFSCTSPAVHR
                     RLRAGRPTTTDRVVPPAIDPAAVGHSDDRHVLLAEPGSASGTGDRWELRVDTAHPTFFD
                     HPVDHIPGMVLLEAARQAALVATGMPDALLLGLKSNFARYAEFDAPCWIEPQAEPHGTE
                     GGVLVRVRGTQHAETVFTAELVLSPRGR"
     misc_feature    2866..2879
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.03"
                     /tool="antismash"
     misc_feature    2867..2880
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 14.65"
                     /tool="antismash"
     misc_feature    3166..3180
                     /note="TFBS match to SypG, Regulator of biofilm formation
                     and host colonization, confidence: weak, score: 18.62"
                     /tool="antismash"
     gene            3229..4023
                     /locus_tag="IE289_RS45310"
                     /old_locus_tag="GCM10010317_093300"
     CDS             3229..4023
                     /GO_function="GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1008: response
                     regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740558.1"
                     /locus_tag="IE289_RS45310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_093300"
                     /product="response regulator transcription factor"
                     /protein_id="WP_168509673.1"
                     /transl_table=11
                     /translation="MNRQAVDLLDARSQRNLPHRPTIPQPGRAVAPQTAADHPGPPAGA
                     LRVLVVENDVRAADSLVQGLLRQGYAAQSVATGTKALQAHRDADLLLLDLDLPDLDGLE
                     VCRGIRAVCDTPVIAVTARGSELDRVLGLQAGSDDYLVKPYGFRELLARMEAVMRRVRQ
                     QPAAAQVITHGPLRIDVGTRVATLNGKPVDLTRKEFDLLHLLASQPGTVMPRRQLMAQV
                     WDDTWSHRGRTIDTHVSSLRGKLGSSSWIITVRGVGFRLGHP"
     misc_feature    3676..3678
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(4189..4204)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: medium, score: 23.05"
                     /tool="antismash"
     gene            4234..4854
                     /locus_tag="IE289_RS45315"
                     /old_locus_tag="GCM10010317_093310"
     CDS             4234..4854
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018546903.1"
                     /locus_tag="IE289_RS45315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_093310"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_054236402.1"
                     /transl_table=11
                     /translation="MATQERGTRSRHSILESAARVFDERGYDAASTNDILARTGLTRGA
                     LYHHFPSKEAIAVALVTAHSEALVVPDQAVKLQAVIDLTLEFAQRLQRDPVLRASVRLA
                     VEQTSFSRPPQTAYEQSGAAILALLRQAEEQGEILPGVDIQEATSTIVGAFTGLQLMSQ
                     VYSNREDLPERIGAFWRFMLPGLATPGMLGRLRTIPPPARETA"
     gene            complement(5410..6012)
                     /locus_tag="IE289_RS45320"
                     /old_locus_tag="GCM10010317_093320"
     CDS             complement(5410..6012)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740556.1"
                     /locus_tag="IE289_RS45320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_093320"
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="WP_190174081.1"
                     /transl_table=11
                     /translation="MQTRSEQTRQALVRAAAELIANGRLSDAGLVNICRRAGVSRGALY
                     HHFSSTAELTAAVYEQAHERVVALMDEAFEGPVEDAPERLLVALCEALRTEELVRAGIQ
                     LAVDGSAGPPRLRDDVLALVRRRVVDAHRDVVPAPEDLAELAVVVAAGLESLGHTDAEW
                     WDPKTSKRLWVLLRPLFSLAGEGLAEERRVGSGSEGG"
     misc_feature    6289..6304
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.86"
                     /tool="antismash"
     gene            6324..7523
                     /locus_tag="IE289_RS45325"
                     /old_locus_tag="GCM10010317_093340"
     CDS             6324..7523
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF019350.5"
                     /locus_tag="IE289_RS45325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_093340"
                     /product="sensor histidine kinase"
                     /protein_id="WP_190174082.1"
                     /transl_table=11
                     /translation="MSSTTAPTAPTSTTAPTSPTGPIPPVTAAIPLVGSGFGEALHEGL
                     VRAAARMSSRPGHSPLMSSATTLGSWETGAVLDRFTARLPTALAPAEAEVPTLRTTLTE
                     FARTALARAADTTPAPIAPPAASPHQVAAAVALLLECALLHVLENGIAHHESALPRAVA
                     VVRRLGEVVREAGEGMWSGDDGRGERRRLARQLHDELGGALSLARLSLDAAATCAPVRT
                     GAPTPGSPGAVAAHLATAQRALAAAARENQALIDGVRHRADLPPLREALEAFLAGPRPI
                     DGRRAQASVEVIGDEWTLSERHREETFLALREALRDRLTRSGARRVDTVVRITRRWLYA
                     KVTDDGQGPAAGSAGSADSARALRSLTDRVEDLGGRTGITPGTPTGTLLEIHLPLRRHP
                     "
     tRNA            complement(7657..7743)
                     /anticodon=(pos:complement(7707..7709),aa:Leu,seq:cag)
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /locus_tag="IE289_RS45330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /old_locus_tag="GCM10010317_t000860"
                     /product="tRNA-Leu"
     gene            complement(7657..7743)
                     /locus_tag="IE289_RS45330"
                     /old_locus_tag="GCM10010317_t000860"
ORIGIN
        1 gtagctggtg gcgggggtgt caccgcccaa agcgaacgcc tcgtccgccg cccggacatg
       61 cgacgcgtcc ctgtctggtt ccgcatacac ggcaacgctc gcgatcccgg catcccggca
      121 cgcccgggcc acgcggacag cgatttcgcc acggttggcg atgagcacct tgcgcacgat
      181 gaacctcccc agactccgct cggagccaca agactgttcg agtgcgcact tctccttcaa
      241 tacggtccgt tgcagaactg ggtcagaatc cgcagacccg gggcagcact taccggaaga
      301 ggaaagagga aggtcagacc tccgtcaaat agccggacct ggcggacgga tccgccgggt
      361 ccggaaagaa aaggaatgcg ccattccgcg gagaatttcg aaggacgtac gaagggcgtt
      421 cggaggcgtt ccgggacggc gttcgggagg ggttccggag cggcgttcgg gaggggttcg
      481 ggagggggtc gggaggggtt cgggaggtgc ttcccgccgt cccgcggggt ttcgcgggac
      541 ggtcgaccac ttggccagtt gaacacgtga ccggttgaac actggccggt cgagctctcg
      601 ggctcgtccc cggtcaaccg tcccgcaggg ccttgcggta ccagggcgcc gcgaggggga
      661 aatcggtgtc gaaaccggcg ccgggtgcgc gacgcaggtc ggaccagagc ggggtgctgt
      721 cgaagacgtg gtcggtggtg aacatgtcga gtccggacgg aggtacgccg ttctcggcca
      781 ggaccgcccg ggctcggtcg acggtcagtt ccctgtccgg ccagtcggcc ccggtgcact
      841 cggcgaccgt gcgcagaagg gtgtccacgg ggaggggctc cgggtgggtg gcgtggtaga
      901 cggaggcggt caggtcggcg gcgggggcga gcgcggtggc caccagcagc gtggccagtt
      961 cgggtacggc gatcgcggag ctccgggcgg gccagccctc caccgtgccg ggcagcacgc
     1021 gcagcatccg ggtcaggccg ggcaccaccc acctgtcgcc ctccccgtac accaggtgcg
     1081 ggcgcaggac gacaccgccg gccgcgagaa cggtgtcctc ggccgcggcc cgggtgcggg
     1141 acgtgggcga gcccgggttg cggcgcagtt cttcgggccg gtcgccgcgg aaggtgccac
     1201 ggccgtagac ggacgcggtg ctcagctgaa cgatgcgcgc aacccccgcg cggcgcgctt
     1261 cctcgatgag cgccgccgtg ccacgggcgt tgacggcctc gttggcctcg acaccgccgc
     1321 cgatctgcga ggcgcagtgg atcaggacgt cgacgccgtc gcacaccccg cgcaaggacg
     1381 cggggtcggt cagatcggcc cgtacgacgc gagcaccgtc atgggccgca ggaccctcat
     1441 ggggcggggg accctcctgg gtcggctgac gggagccgag caggggactg cgatgggaca
     1501 tgagcctcaa gtcggcccgg tgccgggccg cttcacgggt gacgtgtccg ccgacgaagc
     1561 cggcggcgcc ggtgatcagg atggtgggca cgggtctgtc tcctcgggat ggggacgctc
     1621 tcccaggcgt acggaggatg cgcgggctca gcggccgcgc gggctcagga cgagctcggc
     1681 ggtgaagacc gtctcagcgt gctgcgtacc gcgcacccgc accagtacgc cgccctccgt
     1741 accgtgcggt tcggcctgcg gctcgatcca gcagggcgcg tcgaactcgg cgtagcgcgc
     1801 gaagttgctc ttgagaccca gcaggagggc gtccggcatc ccggtcgcca cgagggcggc
     1861 ctgccgggcg gcttccagca ggaccatgcc tggtatgtga tcgacggggt ggtcgaagaa
     1921 ggtggggtgg gcggtgtcga cgcgcagttc ccagcggtcg ccggtgccgg aggccgatcc
     1981 gggttcggcg agcagcacgt gccggtcgtc ggagtggccc accgcggccg ggtcgatcgc
     2041 gggcggcacg acgcggtcgg tggtggtggg ccggccggcg cgcagcctgc ggtggacggc
     2101 gggactggta cagctgaacg cggcgcccgc ggtggccagc gcccttccgt cgcgcagcac
     2161 ggtgacgtcg tagcgcatac cgccgagcac ccggcccctg cggacgatgt cgcggcagac
     2221 cgtgcgcagt tcgacctccg tggggacggc gtccgcgacg aggagttcgg gggaggtgct
     2281 gaagaacatg tcccacatca ggaactggtg cccgaagggg acgccgaact ccgcgtgcgc
     2341 gagcagggaa ccgatctggc ggacggactc gatcaggagc atcgggtcct ggtagccgcc
     2401 ggactgggtg aagaaagagt ggctgcgcgg ccattgggcg ctgaccgcga attcgtccgg
     2461 gtcgggtccg gctggttcgg cggcggcttc ccagccggtc agcagcacct ccgagacggc
     2521 ggcgcgatgg acgtactcgc gcggaaccgt cgtggtcagc cgcggcgtca gagtgcgcgc
     2581 ggcggtctgg acggaggctt gggcagaggt ctgggcagag gtctgggcag aggtctgaac
     2641 ggcgggttga acggcgtttc gggcagcggg tcgtgaggcg gttcgtgagg cgctggtgcc
     2701 caccgtcgcg acgtcgtctt cgacggacac acgggtggag gtggtgtacg acatgtacgg
     2761 cactcctgtc tgtttccgag cagggggcag caatccgtac cgccggcagc caccccccgt
     2821 ggccgacgtt ctctcgctca cacaagatac agacatgtct gttcggtttt tcaagtaaag
     2881 ctttagcatc cttcgagcga tgagccgccg ctacgggccg gtacagcacg attgcgccac
     2941 cggcgacgcc cgatctttca aaccgtgcag acctgcatgg ttagcgccaa ggacacgcgc
     3001 cggacaaggg gctccacccc caccgcaacc tgccggaaac atgaccttga agcgctggat
     3061 ctctccacat acacgcaggt caacgtgggt agattggaaa aggtacggac cgacgcgcgg
     3121 gggatttcga acgcgttgac aatcagacaa gtctgtttgc tatcgtcctc attgtgagag
     3181 ttcagcagtc cgttccgtga tgacgagact gtgcctcagg aggagcagat gaatcggcaa
     3241 gccgtggatc tactcgacgc ccgatcgcag cgcaatcttc cgcaccgccc cacgattccc
     3301 cagcccgggc gggccgttgc cccacagacg gccgccgacc accccggccc cccggccggc
     3361 gcactgcgcg tcctggtcgt agagaacgat gtcagggccg ccgactccct cgtccagggc
     3421 ctgctgcggc aggggtacgc cgcgcagagc gtggccaccg gcaccaaggc gctccaggcg
     3481 caccgcgatg ccgatctcct actgctcgac ctggacctgc ccgacctgga cgggctcgag
     3541 gtgtgccggg gcatccgggc ggtctgcgac accccggtca tcgccgtcac cgcgcgcggc
     3601 tccgagctcg accgggtgct cggactgcag gccggctcgg acgactacct ggtcaagccg
     3661 tacggcttcc gcgagttact ggcccggatg gaggcggtga tgcgccgggt gcgtcaacag
     3721 cccgccgccg cacaggtcat cacccacggg ccgctgcgca tcgacgtcgg cacccgggtg
     3781 gccaccctga acgggaagcc ggtggacctg acccgcaagg agttcgacct gctgcatcta
     3841 ctggcctcgc agcccggcac cgtcatgccg cgacgccagc tcatggcgca ggtctgggac
     3901 gacacctggt cccaccgcgg gcgcaccatc gacacccacg tcagcagcct gcgcggcaag
     3961 ctcggttcga gcagttggat catcaccgta cgcggtgtcg gttttcggct cggccacccg
     4021 tgagcggcac ccgcaggccg tacgaggacc acgtaagggc cgcttgaaga ccactcgagg
     4081 tccacttgag aagcactcga tgaacggccg aggaccgacc gaaaatcgag ccaaaccgga
     4141 agcaaatact cccgcctact ggcggcgtgc cgccttgccg atacaataca gaccagtcgg
     4201 taaaattcca gtgggcgaga gcaaggacct ggtatggcca cacaagagcg cgggacgagg
     4261 tcgaggcact cgatcctgga gtccgcggcc cgcgtgttcg acgagcgcgg ctacgacgcg
     4321 gcgagcacca acgacatcct ggcccggacg ggcctcactc gaggcgcgct ttaccaccac
     4381 ttcccctcca aggaagcgat cgccgtggcc ctcgtgaccg cgcacagcga ggcgctggtg
     4441 gtgccggacc aggcggtgaa gctccaggcc gtcatcgacc tcaccctgga gttcgcccag
     4501 cgtctccagc gcgatccggt gctccgggcc agcgtgcggc tcgccgtcga gcagacgtcg
     4561 ttctcgcggc cgccgcagac ggcgtacgag cagtccggcg ccgccatact cgccctgctg
     4621 cggcaggccg aggagcaggg cgagatcctg ccgggcgtgg acatccagga ggccacgtcg
     4681 accatcgtcg gcgccttcac cggcctgcag ttgatgtccc aggtctacag caaccgcgag
     4741 gatctgccgg aacgcatcgg tgcgttctgg cgcttcatgc tgcccggcct ggccacgccc
     4801 ggcatgctcg gccgcctccg caccatcccg ccgccggccc gcgagacggc ctgaccctcg
     4861 cccgacaccc cacaaccaaa tcccacaacc gagcctcgcg ccctgcccac accctgcacc
     4921 gcgccctgct ccgcccgccg aacagcacac gtcgccccga acgcgaactc ccgggcgggg
     4981 ccgcctcacc cgtgacggac ccgagcccga cacctcaacc gggccaaacc ccccaccacg
     5041 ggtccccttc acatgccacg ggtccccttc gcatgccgtc ccgttcacgg gtacgccccg
     5101 catcggacct ccgaaggccg agggcgcctt ctcgcgccgc cccgaacacg ggcaccgccc
     5161 cgcaaccgac ctctggacac cgtgggctcc acccacaccc gggctccggc cccctcccgc
     5221 ctcccgggcg ggccggtgtc ccccgggctc ccggacgggc cggtgtcccc ggatcctggg
     5281 ccgtgcaccc aagccctgcc gagttccgcg cgccggccct caggccaact ccgcccagca
     5341 cgcgcgacct tgaggggacc ccagaggccc gccctcaccc ccgcttcgga actcggcccc
     5401 cttcggaact cagcccccct cggacccgct ccccacccgc cgctcctccg ccagcccctc
     5461 cccggccaac gagaacaacg ggcgcaacaa cacccacaac cgcttggacg tcttggggtc
     5521 ccaccactcc gcgtccgtgt gcccgagcga ctccagcccc gcggccacca ccaccgccag
     5581 ctcggccaga tcctccggcg ccggcacgac gtcccggtgc gcgtcgacca cccgcctgcg
     5641 caccaacgcc agtacgtcgt cacgcaaccg aggcggcccc gccgacccgt ccaccgccag
     5701 ctgtatgccg gcccggacca gttcctccgt acgcaacgcc tcgcacaagg cgaccaggag
     5761 gcgctccggg gcatcctcca cgggcccctc gaaggcctcg tccatcagcg cgacgacccg
     5821 ctcgtgcgcc tgctcgtaca ccgcggccgt caactccgcg gtggaggaga agtggtggta
     5881 aagagccccc cggctcaccc cggcccggcg gcagatgttg accagtcccg catcggagag
     5941 ccggccgttc gctatcagtt ccgccgcggc acggacgaga gcctgacggg tctgttcgga
     6001 ccgggtctgc atcaggctgc acctgcctat tcctgctctt ggcatgaagg agcgcgcggg
     6061 tgcctcgaag gcgccagcaa caaccggaat catacaacca tgcctgtttt agtcgacagc
     6121 cctgtgaacc cagacctcca tccaatcccc ataaatcctg cagctcttgc ccatggacgg
     6181 actcttgacg gccggacgat cccgtgacca caccccatcc ggaacgcccg tccgctcttg
     6241 acaatcagac aagtctgttt gttacgttgg cgatggccac gttgccccgg cacccaagcg
     6301 tcacccgagc gtcgaggagt ccgatgagca gcacgaccgc ccctaccgcg cccacctcca
     6361 cgacggcgcc gacctcaccg accggcccga tcccccctgt caccgcggcc atccccctgg
     6421 tcggcagtgg attcggcgaa gccctgcacg agggcctggt ccgcgcggcg gcccggatgt
     6481 cgtcccgccc gggccactcc cccctcatgt cgtccgccac caccctgggg tcctgggaga
     6541 ccggcgccgt cctggaccgc ttcaccgccc gcctccccac cgcgctcgcc cccgccgagg
     6601 cggaagtgcc caccctgcgc accacgctga cggagttcgc ccgcaccgcc ctggcccgcg
     6661 ccgcggacac cacccccgcc cccatcgccc cgcccgccgc ctccccgcac caggtggccg
     6721 ccgcggtcgc cctgctcctc gagtgcgccc tgctgcacgt cctggagaac ggcatcgcgc
     6781 accacgagag cgcgctgccc cgcgcggtcg cggtcgtccg gcgcctcggc gaggtcgtcc
     6841 gggaggcagg ggagggcatg tggtccggcg acgacggccg cggcgagcgc cgccggctgg
     6901 cccggcagct gcacgacgaa ctgggcggcg ccctgtccct cgcccgactc agcctcgacg
     6961 ccgccgcgac ctgcgccccc gtcaggaccg gcgcacccac ccccggctct cccggcgccg
     7021 tcgcggccca cctcgccacc gcccaaaggg cactggccgc ggcggccagg gagaaccagg
     7081 cgctcatcga cggcgtgcgt caccgcgccg acctgccgcc cctgcgcgag gccctggagg
     7141 ccttcctcgc gggccctcgt cccatcgacg gccgccgtgc ccaggcctcc gtcgaggtca
     7201 tcggtgacga gtggaccctc tccgagcggc accgggagga gaccttcctc gccctgcgtg
     7261 aggccctgcg cgaccgcctg acccgctcgg gcgcgcgccg cgtagacacc gtcgtgcgca
     7321 tcaccaggcg ctggctgtac gcgaaggtga ccgacgacgg ccaaggcccc gcggcgggct
     7381 ccgccggctc cgccgattcc gcgcgggccc tgcgctccct caccgaccgc gtcgaggacc
     7441 tcggcggccg gaccggcatc accccgggca cccccaccgg caccctgctg gagatccatc
     7501 tgcccctgcg cagacacccc tgagccccgc ccgaggaaaa ctgagagaca gaacacactc
     7561 cgaccagcgc cccggcccgc cccactccgg cccgccccga acccgtaccg gagacatgga
     7621 aaagggccca tcgtcttgac gatgagccct tttcgctggt gcgcgagggg ggagttgaac
     7681 ccccacgccc ttgcgggcac tggaacctga atccagcgcg tctgcctatt ccgccacccg
     7741 cgcattgggt gtgt
//
