LOCUS       NZ_BMUQ01000013        10210 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces mirabilis strain JCM 4551 sequence13, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUQ01000013
VERSION     NZ_BMUQ01000013.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces mirabilis
  ORGANISM  Streptomyces mirabilis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 317459)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 117X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650275.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:52:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,392
            CDSs (total)                      :: 10,311
            Genes (coding)                    :: 9,898
            CDSs (with protein)               :: 9,898
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 413
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 172 of 414
            Pseudo Genes (incomplete)         :: 329 of 414
            Pseudo Genes (internal stop)      :: 40 of 414
            Pseudo Genes (multiple problems)  :: 115 of 414
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:21:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 2942
            Orig. end                         :: 13152
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUQ01000013.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10210
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10210
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10210
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:5210](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5210
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            complement(1045..2061)
                     /locus_tag="IE289_RS28850"
                     /old_locus_tag="GCM10010317_059460"
     CDS             complement(1045..2061)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1205: ABC
                     transporter, carbohydrate uptake"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007383867.1"
                     /locus_tag="IE289_RS28850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_059460"
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="WP_190171813.1"
                     /transl_table=11
                     /translation="MSRISRLPSPLLRAAACTGVAALALTACGSGSGSGTASTGSGSVK
                     VGLITKTDTNPFFVKMKEGAEKAAKENGAQLSTAAGKFDGDNAGQVTAIENMVAAGVKG
                     ILITPSDSKAIVPAIQKARAKGVLVIALDTPTEPESAVDALFATDNLKAGQLIGEYAKA
                     AMKGKTAKIAALDLAPGVSVGVQRHNGFLKGFGATDKDVVCAQDTGGDQAKGQTAMENC
                     LQKAPGINVVYTINEPAALGAYTALKAKGREKDVLIVSVDGGCTGTQAVKDGKIAATSQ
                     QYPLKMAAEGVKAVVTYAKDGKKASGYTDTGVTLITDKAQDGVTSKDTAYGLDNCWG"
     misc_feature    complement(2183..2196)
                     /note="TFBS match to RpoN, Regulator of nitrogen
                     assimilation and virulence, confidence: medium, score:
                     16.73"
                     /tool="antismash"
     gene            2309..3271
                     /locus_tag="IE289_RS28855"
                     /old_locus_tag="GCM10010317_059470"
     CDS             2309..3271
                     /EC_number="2.7.1.-"
                     /GO_function="GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1276: PfkB domain protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987107.1"
                     /locus_tag="IE289_RS28855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_059470"
                     /product="carbohydrate kinase"
                     /protein_id="WP_190171814.1"
                     /transl_table=11
                     /translation="MSPRQIAVLGECVADAFTEPASVPDELALRVLPGGGPANTAVALA
                     RLGTPARFLARLSGDVFGRLFRAHLEASGVDLSYAVAAAEPSTLAVAELDAEGQAAFSF
                     HAQATADWQWTSAELVGVDLADTACVHTGSLALVKEPGAAVLEDFLAAAAPRATISIDP
                     NVRPLLVHPEVYRARLAHWCALADVLRLSEDDLELLLPGTPPEQACDIWHAAGARLVVI
                     TRGADGALASLDGERVQVPAVATPVVDTVGAGDSFTAGLLHHLGTHGLLGGRLTDLRLG
                     DVEAACLFAAQVAALTCAVAGPNPPWPNQLAQFATVGDA"
     gene            3604..3906
                     /locus_tag="IE289_RS28860"
                     /old_locus_tag="GCM10010317_059480"
     CDS             3604..3906
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009715607.1"
                     /locus_tag="IE289_RS28860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_059480"
                     /product="putative quinol monooxygenase"
                     /protein_id="WP_099922139.1"
                     /transl_table=11
                     /translation="MKKTLLAEFTAREGAEDEVARLIRDYALKVREEEGNLAFDVYTKA
                     ANPRGYWIFEVYRDEDAFKAHLDAPYGAPFNAALTPLIEEDASVLTFLDPLATHP"
     gene            complement(4069..4221)
                     /locus_tag="IE289_RS28865"
                     /old_locus_tag="GCM10010317_059490"
     CDS             complement(4069..4221)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE289_RS28865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010317_059490"
                     /product="hypothetical protein"
                     /protein_id="WP_190171815.1"
                     /transl_table=11
                     /translation="MSLYATTVPLAEGKQVAFAGLPDIERMHIFAAAISRPQALSCSAA
                     PARPG"
     gene            <4440..4640
                     /locus_tag="IE289_RS28870"
                     /pseudo=""
     CDS             <4440..4640
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950677.1"
                     /locus_tag="IE289_RS28870"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="DUF2000 family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="RALPRAVFTSDLFATGNDRDNRAAVRAAVRAAVRAVPTEELDLVG
                     LAVYGPRNAVDKALKGARMHT"
     misc_feature    4851..4864
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.35"
                     /tool="antismash"
     misc_feature    complement(4971..4986)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 22.09"
                     /tool="antismash"
     gene            5001..5210
                     /locus_tag="IE289_RS28875"
     CDS             5001..5210
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311933.1"
                     /locus_tag="IE289_RS28875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /protein_id="WP_099922141.1"
                     /sec_met_domain="strepbact (E-value: 9.7e-16, bitscore:
                     52.3, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSAPQVQTLEISDAELDTVSGGLSPQVGIAAGPTAISSSDLVGQ
                     FGAVKDGVLDTVGQYHQVGATVAF"
     gene            complement(5668..6069)
                     /locus_tag="IE289_RS28880"
                     /old_locus_tag="GCM10010317_059530"
     CDS             complement(5668..6069)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004985256.1"
                     /locus_tag="IE289_RS28880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_059530"
                     /product="hypothetical protein"
                     /protein_id="WP_190171816.1"
                     /transl_table=11
                     /translation="MTIRPSARWRARVDDEARELASGARDPSDAFLSELFPESLLTATD
                     KALHAFESDVQTLQDQADEQIFEAVERVVRTLNEINEVHDGAGYETEEREELCLYIDQT
                     LTEHGIDVPALAVRRGTSRTEITDDWRDW"
     gene            6470..7267
                     /locus_tag="IE289_RS28885"
                     /old_locus_tag="GCM10010317_059540"
     CDS             6470..7267
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE289_RS28885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010317_059540"
                     /product="hypothetical protein"
                     /protein_id="WP_190171817.1"
                     /transl_table=11
                     /translation="MNWLTSLPAVVLVVGGLLLALLVAAVARVGVRALVPVEERDRVPQ
                     IATPLMPTLGAAFAIFAALTLASEAGYLRAAEGLVSDEAAAASRLAWAATSPRVQSEPI
                     HAALLDYLRTTRAREWEASAAASGDDPATAHAIARLERSVRAEAARPEVGTPTGTELVV
                     SMDGVTSSRRARVAAASRDVPALYVVTLVVSGLALIVNSGALVFRSSLRTSLLIVGVAS
                     VVGLSLALLFALSAPWSGPFIVSGHPLDAVIGDLKSGFFDGGP"
     misc_feature    6530..6532
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            <7290..7565
                     /locus_tag="IE289_RS28890"
                     /pseudo=""
     CDS             <7290..7565
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018471709.1"
                     /locus_tag="IE289_RS28890"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="tyrosine-type recombinase/integrase"
                     /pseudo=""
                     /transl_table=11
                     /translation="GDWVQARKAAGVTAELHFHDLRHTGNTPASTAGAGTRELMRRMGH
                     SSSRAPLIYQHMTSDRDRAIADRLGAMIRDGGGDPSGKVDKDSTRT"
     gene            7789..8088
                     /locus_tag="IE289_RS28895"
                     /old_locus_tag="GCM10010317_059560"
     CDS             7789..8088
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020115652.1"
                     /locus_tag="IE289_RS28895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_059560"
                     /product="hypothetical protein"
                     /protein_id="WP_229889242.1"
                     /transl_table=11
                     /translation="MSAGDVRLDGVMERVEAELFSDGGNDAVVRMPGRWFPGVLVQGDS
                     LHILRSDIAEVVESCERGDLDEARHSAGLLLAHLDALPARYEAALGEHGIPRPY"
     gene            <8427..8624
                     /locus_tag="IE289_RS53570"
                     /pseudo=""
     CDS             <8427..8624
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024673.5"
                     /locus_tag="IE289_RS53570"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="IS3 family transposase"
                     /pseudo=""
                     /transl_table=11
                     /translation="GKTYGSPRITLDLREECWRVSENTVTEIMAEFGRQGRKSPADAVP
                     CPIPAGGKSPLTWYGASSTP"
     misc_feature    complement(8661..8675)
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.64"
                     /tool="antismash"
     misc_feature    8773..8787
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.6"
                     /tool="antismash"
     misc_feature    complement(8860..8878)
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 19.99"
                     /tool="antismash"
     gene            8948..9385
                     /locus_tag="IE289_RS28905"
                     /old_locus_tag="GCM10010317_059570"
     CDS             8948..9385
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040000.1"
                     /locus_tag="IE289_RS28905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_059570"
                     /product="hypothetical protein"
                     /protein_id="WP_229889243.1"
                     /transl_table=11
                     /translation="MSGISIKRVSTVAGLAAAAVLAAAPGAWANWSSHITSWTDGGTSR
                     TWADESYSQIQFTGCTAQYATTKQVVVQMWDYDALSSNDYYGAKTFTNCFNGASYTSNG
                     EWTGLPTEPDFYFETSKVGQGGSCCLLNVTSVYVDTTKADS"
     misc_feature    complement(8961..8974)
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: weak, score: 18.46"
                     /tool="antismash"
ORIGIN
        1 tggaggcgac gcccatcagc tggagaccgt tgcggaacac gccgacgatg aggacgccga
       61 tgaaggtgcc caggaccgag ccgcgtccgc cgaagaggct ggtgccgccg aggaccacgg
      121 cggtgatgct gtcgaggttg tcggtctgcc ccgcctgggg gtcgccgact ccggtgcggg
      181 agatgagcag cagggcggcg atgccgtaca gcagacccgc cacggtgtag atgccgatcg
      241 tcagccggga ggtgcggata ccgttcagcc gcgccgcttc ctggctgttg cccagggcgt
      301 agacgtgccg gccccagccg gtgctgctca gcgcgtaggc gagcaggagg aacagggcga
      361 tggtgaccag ggagccgtag gtgatgtcgg tgtggccgag cgggaaggtc tgcccgaggg
      421 ccgtcagcgg gccgggcagg ttggtgaccg tctgctcctc ggagtagatg tgggtcagcg
      481 cgaacgccac gttgagcatg ccgagggtga cgatgaacgg cggcagcggg atcttctgca
      541 ccagcagccc gttgagcagc ccgaagcccg cgcagaccgc catgcccagc gcgatggcga
      601 cgagcggcgg cagggagccc tcggcggcca tcttggcgat cacgatgctg ccgaacgcca
      661 tcacggctcc gcaggacagg tcgatgcccg cggtgaggat gatcagggtc tgtccgatgg
      721 cgagggtgcc gacgaccatc acctgctgca cgatcagcga gaagttgccg ccggtgagga
      781 actggtcggt cgagagggcg aagaaggcgc aggcgagaag gagggcgacc agagggccgg
      841 tggtcggtgc cgtgagcagt ctgcgggccg tggtcggcgc tttgagctcg gcgtacggcg
      901 tggtcgtggc tgtcatgcga agtccttgtc ggaagacaga ggtcccggtg ggaggacaag
      961 ggggcggccg tccccggtcg gggaggtggg gccggccgcc ccgctgaggg gtttggaaat
     1021 ggcggccagg ggcgggtgag cggctcagcc ccagcagttg tccaggccgt aggcggtgtc
     1081 cttcgacgtg accccgtcct gcgccttgtc ggtgatcagc gtgacgccgg tgtcggtgta
     1141 accggacgcc ttcttgccgt ccttggcgta cgtcacgacg gccttgacgc cctcggcggc
     1201 catcttcagc gggtactgct gcgaagtcgc ggcgatcttg ccgtccttga cggcctgggt
     1261 gccggtgcag ccgccgtcga ccgagacgat caggacgtcc ttctcccggc ccttggcctt
     1321 cagcgcggtg tacgcaccca gggccgccgg ttcgttgatg gtgtagacga cgttgatgcc
     1381 gggcgccttc tgcaggcagt tctccatcgc ggtctggccc ttggcctggt cgccacccgt
     1441 gtcctgggcg cacacgacgt ccttgtcggt ggcgccgaag cccttcagga atccgttgtg
     1501 ccgctggacg ccgacggaga cacccggcgc gaggtcgagg gcggctatct tcgccgtctt
     1561 gcccttcatg gcggccttgg cgtactcgcc gatcagctgg ccggccttga ggttgtcggt
     1621 ggcgaagagg gcgtcgaccg cgctctccgg ctcggtcggg gtgtccaggg cgatgaccag
     1681 gacacccttg gcgcgggcct tctggatcgc gggcacgatc gccttggagt cgctcggggt
     1741 gatcaggatg cccttcacgc cggcggcgac catgttctcg atggccgtga cctgacctgc
     1801 gttgtccccg tcgaacttgc ctgccgcggt ggacagctgg gcgccgttct ccttggcggc
     1861 cttctccgcg ccctccttca tcttcacgaa gaacgggttg gtgtcggtct tggtgatcag
     1921 accgaccttg acgctgcccg atccggtgct cgcggtgccc gatcccgatc cggatccaca
     1981 ggcggtcagg gcgagggctg cgacacccgt gcaggcagcg gctctgagca agggggaggg
     2041 cagacgagag atgcgtgaca tgaacgactc ctgcgggatt gcgggcgaac ggccggcatc
     2101 ggggcatgcc gtccctcggt gtcatcgttg acttatgtca tcgttgacac tgcttggcga
     2161 ggatgatgga ctccgttccc cggcaacgtc aatgccttgc acccgtcaca aatcggcaac
     2221 gcccgcggcc gtcgcccgcc cgaagccgtt caccggctgc ccggggcgtg cctgtttctg
     2281 tccgcgcatt ccgagagaag agcagcccat gagcccgcgc cagatcgccg tcctggggga
     2341 gtgcgtcgcg gacgccttca ccgaacccgc aagcgtcccg gatgagctcg ccctgagggt
     2401 gctgcccggt ggcggacctg cgaacacggc ggtggccctg gccaggctgg gtaccccggc
     2461 ccgcttcctg gcacgcctgt ccggcgatgt gttcggccgc ctgttccggg cccatctcga
     2521 ggcgtccggc gtggacctgt cgtacgccgt cgcggccgcc gagcccagca cgctggctgt
     2581 tgccgaactg gacgccgagg gtcaggctgc gttctccttc catgcgcagg ccaccgccga
     2641 ctggcagtgg accagtgcgg agctggtcgg cgtcgacctg gccgacaccg cctgtgtgca
     2701 caccggctcg ctggccctgg tcaaggagcc cggagcggcc gtgctggagg acttcctggc
     2761 tgccgccgcg cctcgggcca cgatcagcat cgaccccaac gtccgcccgc tcctggtgca
     2821 ccccgaggtc taccgcgccc ggctggcgca ctggtgtgcc ctcgcggatg tactgcggct
     2881 gagcgaggac gacctggaac tcctcctgcc cggaacaccg cccgagcagg cgtgcgacat
     2941 ctggcatgcc gccggtgcgc ggctcgtcgt gatcacgcgc ggtgccgacg gcgccctggc
     3001 ctcactcgac ggggagcggg tgcaggtgcc cgcggtggcg acgccggtcg tcgacacggt
     3061 cggtgctggt gactctttca ccgcgggact gctgcaccac ctcggcaccc acggactcct
     3121 cggcggcagg ctgacggacc tgcgtctcgg cgatgtcgag gcagcctgcc tgttcgccgc
     3181 gcaggtcgcg gccctgacct gcgcggttgc cggccccaac ccgccgtggc cgaaccagtt
     3241 ggcgcagttc gcgaccgtcg gcgacgcctg atggtccgtc ggtccgcacc ggagcaatcg
     3301 cgcccgaacc gttgacgcac tggccggatt gcgcttcata atcggacact cgatgtcatc
     3361 gatgacacaa gtcgacgatg acacccccgg ccggcggcgt ggaggccggc ctcggcccgg
     3421 ggctttcggt ccgtccgcgc cggggcggca gtcaccgctg cgcggacagc cgggggacag
     3481 ggagacatcc tgagccctgt acgcgtaacc cggcatgccc gcatgcgagg gatcggctcg
     3541 gtgaatccgg acaaggccgt cgcccggcac ctcgacttcg ctcactgaca aggacacgga
     3601 accgtgaaga agaccctgct cgccgagttc accgcccgag agggagcgga ggacgaggtc
     3661 gcccgcctga tccgggacta cgccctgaag gtgcgggaag aggaaggcaa cctggccttc
     3721 gacgtctaca ccaaggcggc aaacccgcgc ggatactgga tcttcgaggt ctaccgggac
     3781 gaggacgcct tcaaggcaca cctggacgcc ccgtacggtg ccccgttcaa cgccgccctc
     3841 accccgctga tcgaggagga cgcctccgta ctgacgttcc tcgatccgct ggccacgcat
     3901 ccgtaacagg cgctgaacac cggcggcgac ccggagctgg agcgcggaaa gatcgaaccg
     3961 ctgctccaac tccgggtcga cggggtttgt acgcgtgcat gtaccaccgg gtcgtggagg
     4021 tgccggagca gttgtggcgg aacggcccgc cgcgctctgc tttgctggtc agccgggcct
     4081 ggccggcgcg gcagagcagg acagggcctg cggtcggctg atcgcggcgg cgaagatgtg
     4141 catgcgctcg atgtcgggga gaccggcgaa ggcgacctgc ttgccctcgg ccagcgggac
     4201 cgtcgtcgcg tagaggctca cgttgttgct cagcggtccc gtggctgtgt acaggtaggg
     4261 ggagcagggg gcacccggcc ttgggtcaag ccccgtgcac ggatggggct cacctctgta
     4321 ccggttcgcc gatccttggc tttcctctcc ttgcgctggg ccacaccgta tgcggaccac
     4381 ggaccctgat ggactgggca gcgggtgggg cacgggcacc ttctgatccg tagcggtgcc
     4441 gtgccctgcc ccgtgccgtc tttacctccg acctcttcgc gaccggtaac gaccgcgaca
     4501 accgtgccgc tgtccgcgcc gctgtccgcg ccgccgtccg ggccgtgccg acggaggagc
     4561 tggacctggt gggactggct gtgtacgggc cgcggaacgc ggtggacaag gcgctgaagg
     4621 gcgcgcggat gcatacgtga ctgaatctgc acagcgaatg cgcaactgcg ccacgggtgc
     4681 gttctgttgg tacggagttg tcctggtcgg agctgccgtg gcaatagctg tcggcttcct
     4741 gtgcggggct acatgtagag aagctgcccc caattcgtgt gcatgcgtgc atggatgagt
     4801 agccgaacgt ttcaactgcc gtgctgggaa cagcgaccga ctggatgccg ggcaccctcc
     4861 gtgatttgat ccttcgcgcc gacggaatcg cgccaagacg tccggaaacg gatggcgcgt
     4921 gcctgcggtc gcggtccacc ggctatcccc agcttggccg ctcccccttg tggaacatcc
     4981 atacgaaggg cagtttcttc atgaactccg ctccccaggt tcagaccctt gaaatctctg
     5041 acgccgagct cgacaccgtc tccggcggcc tgagcccgca ggtcggcatc gccgccggcc
     5101 ccacggcgat cagcagctcg gacctcgtgg gtcagttcgg cgcggtcaag gacggggtcc
     5161 tggacaccgt cggccagtac caccaggtcg gcgccacggt cgccttctga tcgtcagatc
     5221 gcgggacttc ctcatcgcgg gatcgctgat accggcagcc tgaccgccga tcggcgtcca
     5281 cagcgacaag ccccctacct gcgggtcttc gttggtaggg ggcttgatcg ttaggggttg
     5341 cttcttcttg ccctgggtct tgtcgagccg cacggtgccg tctccctggt cgacggccca
     5401 cagagtctcc accggcgccg ggggccagtc gtcttcgacc tcaagccgga agtgcacctt
     5461 cacgtacggg ctgccgtcgg ggttcgtcat gctgcgcaat cagcttggga agctgcggtg
     5521 ttctagtgcc tgttgaggcg ctgacctggg cgaacggctg ggctgtggtc tcgtcgggct
     5581 cggttgcttt caccgtggtt ccccgttgtt ccccgctcga tctggtgcgc ttgtggtgcg
     5641 gcagtggctc acggacttca gcagcagcta ccagtctcgc cagtcatccg tgatctcggt
     5701 gcggctggtg cctcgcctca ctgccagggc cggaacgtcg atgccgtgct ccgtcaaggt
     5761 ctgatcgatg tacaggcaca actcttcgcg ctcttcggtc tcgtagccgg cgccatcgtg
     5821 aacctcgttg atctcattca gcgtccgaac gacccgctcg accgcttcga agatctgctc
     5881 gtcggcctgg tcctggagcg tctgcacatc ggactcgaac gcgtgtagcg ccttgtccgt
     5941 cgccgtcagc agtgactcgg ggaagagttc agacaagaag gcgtcgctcg ggtctcgcgc
     6001 cccggaggcg agttcccttg cctcgtcgtc cacgcgggcc cgccagcgtg cagatggtct
     6061 gatggtcatg ccccggactg aaagcgccag tactgacacg tccgtcttcg tgcacgcttt
     6121 ccaactgtgt tggttgggtg tgtgtacggg ggcgttcacc tgcgttcatg ggcggctcgc
     6181 cactggattg ggatcggctt ccggtctcgg ctgaacggcg gtgaacgagg ctgaatgaga
     6241 cggaaagcgg gaaggacgcg gcggtcgttg tcagtacagc ctcgtactgg gcgtattcgg
     6301 ctgtttcgcc gacgaaaaga gatgccgtgc cgggcgtcgc gagcgtcaaa ggtcttcaac
     6361 ctgtgatcat ggagcgttca ttgaaagtga tcgacgagga gctccacgtc gcggggcccg
     6421 atgtgccgcc gccgcggggc gctcgcggta acgcgctgta ggtgatgtgg tgaactggtt
     6481 gacctcgctg ccggcggtgg tgctggttgt gggcgggctc ctacttgcct tactggtcgc
     6541 ggccgtggcg cgggtcggcg tccgggcgct cgtaccggtc gaggaacggg atcgggtgcc
     6601 gcagatcgcc acgcccctga tgccgacact cggcgctgcc ttcgcgatct ttgcggcact
     6661 cacactggcc agcgaggccg gctacctgcg cgcagccgag gggttggtga gcgacgaggc
     6721 ggccgcggcc tcccgcctgg catgggcggc gacaagtccg cgagtccagt cggagccgat
     6781 ccatgcggcg ctgctggact acttgcggac cacgcgtgcc agggagtggg aggcttcagc
     6841 agcggcgtcc ggtgacgacc ccgccaccgc tcacgcgatc gcacggctgg agcggagtgt
     6901 gcgtgccgag gcggcccggc ccgaggtggg aaccccgaca ggcacggagc tggtggtttc
     6961 catggacggt gtcaccagta gtcgtcgcgc ccgcgttgcg gccgcctcgc gcgacgttcc
     7021 cgcgctctac gtcgtcacgc ttgtggtcag cggactggcc ttgatcgtga actccggcgc
     7081 actggtcttc cgcagcagcc tgcgaacgtc actgctgatc gtaggtgttg cgagtgtggt
     7141 cgggctgagc ctggcgttgc tcttcgcact cagcgcgccg tggagcggac cgttcatcgt
     7201 cagtgggcat ccactcgacg ccgtcatcgg ggacctgaag tccggcttct tcgacggcgg
     7261 gccgtaggcg acgggcgcga tcagtagccg gcgactgggt ccaggccagg aaagcggcgg
     7321 gcgtcacggc cgagctgcac ttccacgacc tgaggcatac gggcaacacg ccggcctcca
     7381 cggccggggc cggcacgcgg gagctgatga ggcggatggg tcacagcagc tcgcgggccc
     7441 cgctgatcta tcagcacatg accagtgacc gcgatcgggc catcgccgac cgcctcggcg
     7501 cgatgatccg cgacggcggg ggagacccct cgggcaaggt cgacaaggac tccacccgga
     7561 cgtagtggca cgcgtgtggc acggcgtcgg aaacggcgaa gggccggcct gggaggtgaa
     7621 tcctcctgaa ccggcccttc tctctgtgcc cccggcagtg cccccggcag gattcgaacc
     7681 tgcgacaccc gctttaggat ttcggtccgg cggcctaggt gaagccctgg cgctcgtgct
     7741 ggtccagcgt gtgtggtcgt ccaaggctgc ctgcgtacac cgtcgttgat gtcagctggt
     7801 gatgtcaggt tggacggcgt gatggagcgt gttgaggcag agctgttcag tgatggcggc
     7861 aacgacgctg tggttcgcat gcccggcaga tggttccccg gagtgctggt gcagggcgac
     7921 tcccttcaca tcctccgcag cgacattgcc gaggtggtgg agtcatgcga gcgcggcgac
     7981 ctggacgaag cgcggcactc cgccggcctt cttctggcgc acctggacgc tttgccggcc
     8041 cggtacgagg ctgcgttggg cgagcacggg attccgcgac cgtactgaac tgcggggcca
     8101 tcacctcggg ttgacgctga acccccgagg gctgccttgc gggggcggac gtggtcggcg
     8161 acgcggttcc ccaggtcact ccagctgtaa cgacagccca ggtgaaccgg gcatctcggc
     8221 tgcggggagc cgtcgttgac cgtggctgac cgcacgatgt ggcacggctg tggcacgcac
     8281 atagggacta cgggcttgga aggcgcatgg tgcgtgtggc gcgacgtaca acctgagcag
     8341 ccggcttgct tgccgcgacc tctgacggag accgacttcg acacatattc gaaggagatg
     8401 gcgtctgaca ctccttcgac cgttcaggca agacgtacgg ctcgccgagg atcacgctgg
     8461 atctgcggga ggagtgctgg cgggtctccg agaacaccgt caccgagatc atggccgagt
     8521 ttggccgaca gggccgcaag tcgcctgccg acgccgttcc ctgccccatc ccagcaggtg
     8581 gaaagtcgcc gctgacctgg tacggcgcaa gttcgactcc gtagctaggg acgtgttggg
     8641 gtgggggcga tatgacggag atcgataccg gtgagggcaa gctctgcctc gccagtgtcc
     8701 acgatgcctt ctcccgccgg gccttggttg cgcgaggggt gagcggcacg acgccgcgct
     8761 ggtcagcgcc gcataacaga tggcgattgc ggcgagagca ccccctccgc tgcaccaagc
     8821 ggaacccaag aacaagccac ggtggctgaa tgttgacgta aagatcattt aaacagtagg
     8881 ttctcgccgt tccacccgga agtgatcatg aagagcgtga cgccggatgg aggactgggg
     8941 aacccgtatg tcgggcatat caatcaagcg tgtctccacc gtggccggcc tggctgccgc
     9001 cgccgttctc gccgcggcgc cgggtgcctg ggccaactgg tcgagccaca tcacctcttg
     9061 gacggatggc gggacctctc gcacctgggc cgacgagagc tacagccaga tccagttcac
     9121 cgggtgcacg gctcagtacg ccaccacgaa gcaggttgtc gtccagatgt gggactacga
     9181 cgcgctgtcg tccaacgact actacggtgc caagaccttc accaactgct tcaacggtgc
     9241 ttcctacacg agcaacggtg agtggaccgg gctgcccacc gagccggact tctacttcga
     9301 aacctccaag gtcggtcagg gcgggagctg ctgcctcctc aacgtgacgt cggtctacgt
     9361 cgacaccacc aaagccgaca gctgaccttc ggtccgaggc gcggcgctgc cccgtggggc
     9421 agcgccgcgc ctcagcccgt cccgagcccc acgaggaacc ccttgaaacc gctcgcccga
     9481 atccggtcca gcagcgcgct gtgggcgtca cccgtcgtct tgctgcttgt gcttgcctat
     9541 tacttcacgg acatcacttc cgacctgcga ctcgacccgc tcgactacgc gcccaccgtc
     9601 gtcgacgcgg tgctgcgcaa cctggtaccc gtggcctacg cggtcgccgc ggcgttgggc
     9661 gcctgggagg cacgcaagct gggcaacgcg gacgtctggc aatggggcgt ggtgcgctcc
     9721 cgctacacca tcgccgcaca caccctcatc cctgtggtca cgctggcctg gctggtcctg
     9781 ctcctgcccg tcctgtgcgg gctgatccag acgggaaccc cgctggacct gtcgggaacc
     9841 ggcctcgtgg tgttcgccat gctgctctgc gcggcccatg ccgtgatcgg gttcggctgg
     9901 ggagtcgcgg tccggcacat cgcggcggtt ccggtgatcg cggtcctcga cttcctggcc
     9961 ctcggctggt ccacagggct tacagtgccc tggccccggc atctcaccag cctctacccg
    10021 ggcaggctgt tgttcggcga actgcccacc gcgccagcca tcgtggcgcc cctcctcgtc
    10081 ggtctcggcg ccgccgcggc ggtggccctg ctctggtcgg gcataccccg gccgtggttg
    10141 cgcgccgctc tcgccggggc actggcgctg gcctgtttcg gcggcgggta cgccatcgtc
    10201 aaggactgga
//
