LOCUS       NZ_BMUQ01000008        15943 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces mirabilis strain JCM 4551 sequence08, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUQ01000008
VERSION     NZ_BMUQ01000008.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces mirabilis
  ORGANISM  Streptomyces mirabilis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 406960)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 117X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650275.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:52:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,392
            CDSs (total)                      :: 10,311
            Genes (coding)                    :: 9,898
            CDSs (with protein)               :: 9,898
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 413
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 172 of 414
            Pseudo Genes (incomplete)         :: 329 of 414
            Pseudo Genes (internal stop)      :: 40 of 414
            Pseudo Genes (multiple problems)  :: 115 of 414
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:21:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 15943
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUQ01000008.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..15943
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..15943
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..15943
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[3395:5943](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3396..5943
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     gene            129..1262
                     /locus_tag="IE289_RS20810"
                     /old_locus_tag="GCM10010317_042840"
     CDS             129..1262
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018958865.1"
                     /locus_tag="IE289_RS20810"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_042840"
                     /product="sensor histidine kinase"
                     /protein_id="WP_190170689.1"
                     /transl_table=11
                     /translation="MGDVSRRRLVLLDGAAALGFALLPQLTLVRPDSGGAPGGLTVSLL
                     SFATALPLAVRRLWPVPVFAIVLVASCVALVAGLGPAVFLSAAYALYLVATTRRRQLGR
                     SAVIVGGASTAGAGLLTVTGGQHYTGGTLAVQLLFGLLVLGATWAAGTAVRERRESLRR
                     TIVQAAEQAKVEERMRIARDIHDVVTHSVGLIAIKAGVANHVIATHPEEAREALTVIED
                     ISRRALRDMRATLKVLRREHVDQQQDLQPVPGLSDLPSLVHTAEAAGVSVDLRSVCAEE
                     PPDGVALTAFRIVQEALTNVVKHAAPTRCLVSVTAQDGVLTIGVTDDGPGPGHRPTVPG
                     GDMGLVGMRERAVAHGGTLTAGPRPGAGFRILATLPY"
     gene            1409..2332
                     /locus_tag="IE289_RS20815"
                     /old_locus_tag="GCM10010317_042850"
     CDS             1409..2332
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014173620.1"
                     /locus_tag="IE289_RS20815"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_042850"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_190170690.1"
                     /transl_table=11
                     /translation="MIDVQNLTKRYGSATVVDGLTFTVRPGAVTGFLGPNGAGKSTTLR
                     MMLGLTRPDAGTARIDGHAYGDLAYPLRHIGALLETSAPHRGLTARDHLRWLAQSNRIA
                     RGRVAEVLEAVGLADAARRRTGTFSLGMGQRLGLAAALLGDPPVLVLDEPVNGLDTEGI
                     RWLRDLLRSMAAEGRTVLISSHVMSEMALVADHLVVISRGRLLADTGMSDFIERHGRSY
                     VRVRTAEPDRLGRELEGRGATVSRVPGGGLDVVGMAAADVSRIAAAGGFPLDELGTHAG
                     SLEETFLDVIGEGHHALGEEGRNTHV"
     gene            2325..3077
                     /locus_tag="IE289_RS20820"
                     /old_locus_tag="GCM10010317_042860"
     CDS             2325..3077
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024141.5"
                     /locus_tag="IE289_RS20820"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_042860"
                     /product="ABC transporter permease"
                     /protein_id="WP_190170691.1"
                     /transl_table=11
                     /translation="MSETLAAVRAETTKLRGIRGTQISLLLFAAVSVLIAALDGWSAKN
                     ALESDNPSLRSDFTPEQAGLDGILYGQLALIVFGVLVVTSEYTSGMIRVSLLAVPRRGR
                     LYAAKTVVTALAAVAVSVPVTVVGYLVTQAALGSHGSSLGASGVPRALAGAVVYLTLMC
                     LFAAGIAAIARNAVVPLAVLLPMVLAGTHILSLIGATKEMARYFPDQAGNELLTVRSPH
                     TAFGFTVLLAWTVAALGWGWVRQRRWDS"
     gene            3194..3322
                     /locus_tag="IE289_RS20825"
                     /old_locus_tag="GCM10010317_042870"
     CDS             3194..3322
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_103079660.1"
                     /locus_tag="IE289_RS20825"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_042870"
                     /product="aborycin family tricyclic lasso peptide"
                     /protein_id="WP_190170692.1"
                     /transl_table=11
                     /translation="MSAIYEPPMLQEVGDFEELMKCLGVGSCNDFAGCGYAIVCFW"
     gene            3396..5237
                     /locus_tag="IE289_RS20830"
                     /old_locus_tag="GCM10010317_042880"
     CDS             3396..5237
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_067372303.1"
                     /locus_tag="IE289_RS20830"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_042880"
                     /product="lasso peptide isopeptide bond-forming cyclase"
                     /protein_id="WP_190170693.1"
                     /sec_met_domain="Asn_synthase (E-value: 4.6e-38, bitscore:
                     126.3, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFVVLPDCPAGAAAAGRLRATRRVDHASGRPWIVGDWPEAEAVV
                     VEAGPRRMVVLGRTRLDETAATAALGRLRSLHDVDAIASRLPGAVHLAVSLDGRTRVQG
                     SAVGVRHIFTAVVDGVTVAASGVEPLLRLTGAGLDETVLAARLLAPGGPPWPLAQRPVR
                     RGVEALTTGHWLELDPGGRARQIRWWELPEPSLTLAKGAAAVRSALDDAITTRVAAGGT
                     LSADLSGGLDSTSLCFLAHAAGSDLVTYHVMPIDSANADTMWAHRAAECLPAARHHTLS
                     ADRAENLFDIGYTADLVGAAPEGPSTWASGLAHIQDLAKRATAEGAALHLTGFGGDELF
                     GRMPACAWSLARATPVGGLRLVNRYRLANRWPWRATVRSLLDRSTFARNLGRVAARIDA
                     PPPPVDEPDFGWVFAPRMPAWATPDAVAAVRALLNDAATEGPGPLDADRARHQALASLV
                     FEGTTVRQVNTALGDTGITWDAPFLDDRVVEAALATRIDQRLLGGRFKLLLTSAAEGLV
                     PADILARRDKGEFSAEAFRGLARNRARILELCEDSQLARLGLIDPAAFRSAVLNPGPIS
                     HHLQPIDTTVACESWLRTHPETHPLPPARNTPTGELR"
     aSDomain        5234..5407
                     /aSDomain="Lasso_Fused_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing fused peptidase in a lasso
                     peptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IE289_RS20835_Lasso_Fused_RRE.1"
                     /evalue="1.20E-10"
                     /identifier="RREFam003.1"
                     /label="IE289_RS20835"
                     /locus_tag="IE289_RS20835"
                     /protein_end="58"
                     /protein_start="0"
                     /score="32.3"
                     /tool="antismash"
                     /translation="MKLTLARDVTLTVVDTGAVLLDGRRGRYWQLNHSGAGVLRQLLDG
                     QAPDAAAAGLCAA"
     gene            5234..5497
                     /locus_tag="IE289_RS20835"
                     /old_locus_tag="GCM10010317_042890"
     CDS             5234..5497
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lasso_Fused_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056407.1"
                     /locus_tag="IE289_RS20835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_042890"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_190170694.1"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 1.6e-09,
                     bitscore: 32.3, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 1.7e-24,
                     bitscore: 80.3, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 2.4e-12, bitscore: 41.6,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLTLARDVTLTVVDTGAVLLDGRRGRYWQLNHSGAGVLRQLLDG
                     QAPDAAAAGLCAAAPVSDDQARQDVQALIDALSAAKLVEVAS"
     aSDomain        5240..5488
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IE289_RS20835_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="1.30E-25"
                     /identifier="RREFam002.1"
                     /label="IE289_RS20835"
                     /locus_tag="IE289_RS20835"
                     /protein_end="85"
                     /protein_start="2"
                     /score="80.3"
                     /tool="antismash"
                     /translation="LTLARDVTLTVVDTGAVLLDGRRGRYWQLNHSGAGVLRQLLDGQA
                     PDAAAAGLCAAAPVSDDQARQDVQALIDALSAAKLVEV"
     gene            5494..5943
                     /locus_tag="IE289_RS20840"
                     /old_locus_tag="GCM10010317_042900"
     CDS             5494..5943
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014140854.1"
                     /locus_tag="IE289_RS20840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_042900"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_190170695.1"
                     /sec_met_domain="PF13471 (E-value: 5e-26, bitscore: 85.6,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPAVAEQAPRLPWYRQLAPRCAAGAARLLVRLPPARLHRVLGV
                     VSKGSRPAGYAEVARARRSVVSVSTRCAGLGCLQRSVATVLLCRAHGRWADWCTGFRTE
                     PFGAHAWVEADGRPVDEPGELSVFRTVLAVRRPDERRSTPERSHS"
     gene            5993..6904
                     /locus_tag="IE289_RS20845"
                     /old_locus_tag="GCM10010317_042910"
     CDS             5993..6904
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: protein motif:HMM:NF012235.5"
                     /locus_tag="IE289_RS20845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_042910"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_229889052.1"
                     /transl_table=11
                     /translation="MNGLDLTVPTGSMYGFLGPNGAGKTTTINMLTTLLRPTAGRAEVA
                     GFDVAARPAEVRRRIGIVFQESTLDLDLTAAENLRFQADLCGLSRRASRDAIASMLDLM
                     DLSERRRVPVRQFSTGLRRRLEIARGLLAEPSVLFLDEPTTGLDAQTRAAVWEHLERLR
                     RERGITVFVTTHQLDEAEHCDRIAIIDRGKVVTEGTPADLKSVIGAELVVLRTDDDQRA
                     AEALGDRFGLPAEPTPDGLLLRVDRAAALVPRLCTELGVTVREVAIAPPTLDDVFLHHT
                     GLAIRESPTGPRMLGNLGEGLR"
     gene            6901..7770
                     /locus_tag="IE289_RS20850"
                     /old_locus_tag="GCM10010317_042920"
     CDS             6901..7770
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1065: ABC-2 type
                     transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007385817.1"
                     /locus_tag="IE289_RS20850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_042920"
                     /product="ABC transporter permease"
                     /protein_id="WP_190170696.1"
                     /transl_table=11
                     /translation="MSRTDTAPAALGDITAAPPPADRSRNPARPVLLLWRREMTRLRHN
                     PVRLAMGLVTPLLFLVVLGTGLDAASSSLGKAQLNDYRAYLFPGTLVMSVQAPAIAVGI
                     SLVWDRRLGVLRQMLVAPFPRASIVFGLALGGATTGAVYGLMVLSVGGIASIRYTPMLL
                     VVLLELLLVSLMFTSLGLLAAVTIRQVDTFQVVVNLSLMPLMFFSGAMFPPNGLPGWLD
                     TVVKLNPLTYGVDAVRRTLPGPSVLTSEQTRLMLGDWHPPVAAELGVLAALTAVALGLA
                     GYRFSRTS"
     gene            7767..8327
                     /locus_tag="IE289_RS20855"
                     /old_locus_tag="GCM10010317_042930"
     CDS             7767..8327
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018942.5"
                     /locus_tag="IE289_RS20855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_042930"
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="WP_190170697.1"
                     /transl_table=11
                     /translation="MSRGGQGTVDASATDAGTTDVTRRANTRATTGYWAGAARLAARLL
                     LAAVLAYAGLVKIGDLTEAGRTVALYRIVPDDSAQLAGGVLPFVEVALALLLVAGLATR
                     AAAAGAAVLLVAYVAAIASVWARGMSIDCGCFGGGGTLSGGAARGYALDIARDLVLLGA
                     AALLIRNPRTRYALDSWILDPKE"
     gene            8333..9052
                     /locus_tag="IE289_RS20860"
                     /old_locus_tag="GCM10010317_042940"
     CDS             8333..9052
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013488.5"
                     /locus_tag="IE289_RS20860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_042940"
                     /product="thioredoxin domain-containing protein"
                     /protein_id="WP_190170698.1"
                     /transl_table=11
                     /translation="MTSTQTTDATVREMVHRRHRRRRTVVVSLVAALVVVAAALVGAGL
                     VRANNTAPGKAPSRVPAGIAADKSGVAASTGAVRVDVYLDYLCPECRRTERALTTALDD
                     LRSHGGVSVVYHPVAFLDGRSEPAGYSTQAASAADAGRFEQYSTVLFSKQPAEQGPGLS
                     EAQLIAAGRDAGITAASFARCVEDAPYRPWVRYVSDIAASRKVALTPTVMVAGRRVDVT
                     GSDPGGALTRAVTEARR"
     gene            9049..10371
                     /gene="mslH"
                     /locus_tag="IE289_RS20865"
                     /old_locus_tag="GCM10010317_042950"
     CDS             9049..10371
                     /codon_start=1
                     /gene="mslH"
                     /inference="COORDINATES: protein motif:HMM:NF038314.1"
                     /locus_tag="IE289_RS20865"
                     /note="Involved in the biosynthesis of MS-271 and related
                     lasso peptides; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_042950"
                     /product="lasso peptide C-terminal Trp epimerase"
                     /protein_id="WP_190170699.1"
                     /transl_table=11
                     /translation="MTRLTVALSGDCMATRGAVISSDPAAGRLRELLHGADFAVTNLEV
                     VSSDGRGHPVHNAAGGGCLIADSGVLDEITAAGFTVLGCANNHAMDLGTEGVLGTVDQL
                     RSRRIPFAGIGADLTTARRPVHVDRPGGSLALLACTATFLPGQEAADPSPELPGRPGLN
                     PLRHSATLRVTAPQMDVLRTIDAETGLRARRAEARTLLGVDPAILAPDPLTLFGTRFLT
                     ADEPGFTTECDPRDLDEISRWVGEARLRADLVVASVHSHEPSPTPETPGEFLRAFAHRM
                     IDEGADVVVGHGPHFLRGIELYRNRPIFYSLGNIVSQIELTDRVSAEDYAKVTTDRAPT
                     PGRYYDRLSGHGTRLFAPHRRYWQSLLPVLTFDDGTLVTARLHPVDLGFALPVHRRGRP
                     RLADQAEAKEILTDVTRLSESYGTTVGAGVDGTAELLLDVA"
     misc_feature    10408..10424
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 19.09"
                     /tool="antismash"
     gene            10461..11135
                     /locus_tag="IE289_RS20870"
                     /old_locus_tag="GCM10010317_042960"
     CDS             10461..11135
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020123207.1"
                     /locus_tag="IE289_RS20870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_042960"
                     /product="response regulator transcription factor"
                     /protein_id="WP_190170700.1"
                     /transl_table=11
                     /translation="MAESVRVLIADDQALLRGSFRVLVDSTPGMEVVGEAGDGATAVRL
                     ARELAPDVVLMDLRMPVLDGIAATREICSDETLTGVRVLALTMFDMDEYVYPALQAGAS
                     GFMLKDASPSDLVDGIRVIATGEGVLAPTVTRRVIANFSRRGETPRAAPRLVGLTKREH
                     EVLVQIANGLSNAEISERLVISMPTVKTHVGSLLAKLHARDRAQLVIIAYESGLAERGM
                     PT"
     misc_feature    complement(10462..10476)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     misc_feature    11438..11456
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 18.2"
                     /tool="antismash"
     misc_feature    12290..12302
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 16.61"
                     /tool="antismash"
     misc_feature    12301..12316
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.58"
                     /tool="antismash"
     misc_feature    12432..12447
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.09"
                     /tool="antismash"
     gene            12435..12788
                     /locus_tag="IE289_RS20875"
                     /old_locus_tag="GCM10010317_042980"
     CDS             12435..12788
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE289_RS20875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010317_042980"
                     /product="hypothetical protein"
                     /protein_id="WP_190170701.1"
                     /transl_table=11
                     /translation="MSVSRTAVRLIATCVASGALVGAAAMPALAAGGGHDRDNGRGYSH
                     SHRSDHDHDGHFGDRDRDRGWGWDRGHDRGWDRGHDRDRGWDRGRDRDQDRGGRHGWYS
                     DRHRDQDRYPGHR"
     misc_feature    13261..13271
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     misc_feature    complement(13418..13438)
                     /note="TFBS match to NrtR, NAD synthesis repressor,
                     confidence: weak, score: 19.25"
                     /tool="antismash"
     gene            13542..14963
                     /locus_tag="IE289_RS53160"
                     /old_locus_tag="GCM10010317_043000"
     CDS             13542..14963
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF013508.5"
                     /locus_tag="IE289_RS53160"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_043000"
                     /product="chaplin family protein"
                     /protein_id="WP_229889053.1"
                     /transl_table=11
                     /translation="MANGLGCAISSHVRCCLYAVVIGKEMVEISAVKLSWRTCTTPVSL
                     VALAIASAPLASQAQALVMPLGVNARHAPGTPGAPQAPVAVFAEDFENAGETPIFLEDY
                     VGAPPLNETYTADPPWLDHGQCNGIILDQTGADQPDCPAVLKNMANALGQVGGTNPPTN
                     HVVAAYTNWVPPGADRVEFRTERPIPIAKPNRYITFAVDVAAVNCGQAAPPLLKFYLTG
                     NGSDIPTFTTPINPCADPNSKPYPGGNGLRAGAFAGNRAVLFNDSQLGIKMVNGQGEWF
                     GNDHAFDNIRILDATPQLDKAFSPATVNKGGTSTLTMTVTNTSELAAKNDFSFVDNLPA
                     GVKVAANANASTTCGNGTVSAAAGGASVALNGGDLAAGEKSCTVTVNVTADKAGTYVNR
                     PEAITTVGLNPPDPATLTVRKKGATAVGTATGSGGLLSGNVVQVPVDMPVNACGNSVNV
                     IGLLNPATGNVCVNG"
ORIGIN
        1 aaccagacca acgaacagac aagccaacgg tcacatgatc aacagctttt gttagagcca
       61 gttaggcggt catggtcctg ttccggccgt gttggacgtc cggctgatca ggcgcatagg
      121 ttcgggacat gggagatgtc tcgcgccgac gcctggtcct cctcgacggg gcggcggccc
      181 tcgggttcgc cctgcttccg caactgacgc tggtacggcc cgacagcggc ggtgcgcccg
      241 gtgggctgac cgtgtcgctg ctctcgttcg ccaccgcact gccgctcgct gtacggcggc
      301 tgtggcccgt cccggtgttc gcgatcgtcc tcgtcgcgtc ctgcgtggcg ctggtcgccg
      361 gtctcggccc tgccgtcttc ctctccgcgg cctacgccct gtacctggtc gcgacgacac
      421 ggcgacggca gctggggcgg tcggccgtga tcgtcggcgg ggcgagcacg gcgggcgcgg
      481 gcctgctgac ggtgacgggc gggcagcact acacgggcgg caccctggcg gtgcaactgc
      541 tgttcgggct attggtgctg ggggcgacgt gggccgcggg gacggccgta cgggagcgac
      601 gcgagagcct gcggcggacc atcgtgcagg cggccgagca ggccaaggtc gaagaacgca
      661 tgaggatcgc ccgggacatc cacgacgtcg tcacccacag cgtgggcctg atcgcgatca
      721 aggccggggt cgccaaccat gtcatcgcca cccaccccga ggaggccaga gaagccctca
      781 ccgtcatcga ggacatcagc cgcagggcgc tgcgcgacat gcgggccacc ctcaaggtgc
      841 tgcgccgaga acacgtcgac cagcagcagg acctccagcc ggtccccgga ctgtcggacc
      901 tgccttccct ggtccatacg gccgaggcgg cgggcgtcag cgtcgacctg cgctccgttt
      961 gcgcggagga accacccgac ggggtcgcgc tgaccgcgtt ccggatcgtc caggaggcgc
     1021 tgaccaacgt cgtcaaacac gctgccccga cccgctgcct ggtcagcgtc accgcgcagg
     1081 acggcgtgct gacgatcggc gtcaccgacg acggccccgg acccgggcac cggccgaccg
     1141 tgcccggcgg cgacatgggg ctcgtcggga tgagggaacg ggccgtcgcg cacggcggga
     1201 ccctcacggc gggcccccgc cccggcgcgg gcttccggat cctcgcaaca ctgccgtact
     1261 agtgccacgc caaccgccat acatcccgcc caccgcatat ggctgaaacg gtctcatact
     1321 ccggtatggg tccggtccgc gaaaccggcc cgcaccgtcc cgaccccggt ccgatgtgtg
     1381 gtgcggccgg cgcggccaga ctcgtcgcgt gattgacgtc cagaacctga ccaagcggta
     1441 cgggtcggcc accgtcgtgg acggtctgac cttcacggtg cggcccggag ccgtcaccgg
     1501 cttcctcggc cccaacggcg ccgggaagtc gaccaccctg cggatgatgc tgggcctgac
     1561 ccgccccgac gccggaaccg cgcggatcga cgggcacgcc tacggcgacc tggcctaccc
     1621 cctgcgccac atcggcgccc tgctggagac ctcggccccg caccgcggcc tgaccgcgcg
     1681 cgaccatctg cgctggctcg cccagagcaa ccggatcgcc cgcggccggg tcgccgaggt
     1741 cctggaggcg gtcgggctcg cggacgcggc ccggcgccgg accggcacct tctccctcgg
     1801 catgggccag cggctgggcc tggccgccgc gctcctcggc gacccgcccg tgctggtgct
     1861 cgacgaaccg gtcaacggcc tggacaccga gggcatccgc tggctgcgcg acctgctgcg
     1921 ctcgatggcc gccgagggcc gtaccgtcct catctccagc catgtgatga gcgagatggc
     1981 cctggtcgcc gatcacctgg tcgtcatcag ccggggccgg ctcctcgcgg acaccgggat
     2041 gtcggacttc atcgagcgcc acggacggtc gtacgtccgg gtccgcaccg ccgaacccga
     2101 ccggctcggc agggagttgg aaggtcgcgg cgccactgtg agccgcgtcc ccggcggcgg
     2161 cctggacgtc gtgggcatgg cggccgcgga cgtcagccgg atcgccgcgg cgggcggctt
     2221 cccgctcgac gaactcggca cccacgccgg gtcgttggag gagaccttcc tcgacgtcat
     2281 cggagaaggc caccacgccc tgggtgagga aggcaggaac acccatgtct gagaccctcg
     2341 cggccgtccg cgccgagacc accaagctgc gcggcattcg cggcacccag atctcgctgc
     2401 tgctgttcgc cgcggtcagc gtcctcatcg ccgccctcga cggctggtcc gcgaagaacg
     2461 cgctggagtc cgacaacccg agcctgcgtt ccgacttcac cccggagcag gccggtctcg
     2521 acggcatcct ctacggccag ttggcgctga tcgtgttcgg tgtgctcgtc gtcaccagcg
     2581 agtacacgtc cggcatgatc cgtgtctccc tgctcgccgt accccggcgc ggacggctct
     2641 acgcggcgaa gacggtcgtc accgccctgg cggcagtggc cgtctccgtc cccgtcaccg
     2701 tcgtcggcta cctggtcacg caggcggccc tcgggtccca cggttcctcg ctcggcgcga
     2761 gcggtgtccc gcgcgccctg gccggggcgg tcgtctatct gaccctgatg tgcctgttcg
     2821 cggccggtat cgcggcgatc gcccgcaacg ccgtcgtacc gctggccgtt ctgctgccga
     2881 tggtgctcgc cgggacacac atcctgtccc tcatcggggc gaccaaggag atggcccggt
     2941 acttccccga ccaggcggga aacgaactgc tcaccgtccg ctccccgcac acggccttcg
     3001 gcttcacggt gctgctcgcc tggaccgtcg ccgccctcgg atggggctgg gtgcgccagc
     3061 gccgttggga cagctgacag gacgggcgtg ggacaactgc cccatgccgg tgggacattc
     3121 cgccccgtaa cttccgtgac agatcacagg ccgaacaagg tcggcctgac cgatccgata
     3181 cgggaggacc cccatgtccg cgatctacga accccccatg ctccaggaag tcggcgactt
     3241 cgaggagctc atgaagtgcc tcggcgtcgg aagctgcaac gacttcgccg gctgcggcta
     3301 cgcgatcgtc tgcttctggt gatcacgtcc ggtgccggtg cgttcacacg caccggcacc
     3361 acccggggca acgaggagac ggggaacgag acgacatgga attcgtggtt cttccggact
     3421 gcccggccgg tgccgcggcg gccggccggc tgcgggcgac gcggcgcgtc gaccacgcgt
     3481 cggggcggcc ctggatcgtc ggcgactggc ccgaggccga ggccgtcgtc gtcgaggcgg
     3541 gcccacggcg gatggtcgta ctgggccgca cccggctcga cgagaccgcc gcgacggccg
     3601 cactcggccg gctgcgctcg ctgcacgacg tggacgcgat cgcgtcccgg ctgccgggcg
     3661 ccgtccacct ggcggtgtca ctggacggca ggaccagggt gcagggttcg gccgtcggcg
     3721 tacgacacat cttcacggcc gtcgtcgacg gagtgaccgt cgccgcgagc ggggtggaac
     3781 ccctgctgcg gctgaccggc gccggcctcg acgagaccgt gctcgccgcc cgcctgctgg
     3841 ccccgggcgg accaccctgg ccgctcgccc aacgccccgt ccgccggggc gtcgaggcgc
     3901 tcaccaccgg ccactggctg gaactggacc cgggcggccg ggcccggcag atccgctggt
     3961 gggaactccc ggagccgtcc ctcacgctcg cgaagggcgc ggccgccgtc cgctccgcgc
     4021 tggacgacgc gatcaccacc cgggtcgccg cgggcggcac cctcagcgcc gacctgtccg
     4081 gcggcctgga ctccacctca ctgtgcttcc tcgcccacgc ggccgggtcc gacctggtca
     4141 cgtaccacgt gatgccgatc gacagcgcca acgcggacac gatgtgggcc caccgggccg
     4201 cggagtgcct gccggcggcc cggcaccaca cgctgtccgc cgaccgcgcc gagaacctgt
     4261 tcgacatcgg ctacaccgcc gacctcgtgg gcgcggcccc ggaaggtccc tcgacctggg
     4321 cctccggact cgcccacatc caggacctgg ccaagcgagc cacggcggag ggcgccgcgc
     4381 tgcacctgac cggcttcggc ggtgacgagc tgttcggccg gatgcccgcc tgcgcctggt
     4441 ccctggcccg ggccacgccg gtcggcgggc tgcggctggt caaccgctac cggctggcca
     4501 atcgctggcc gtggcgggcg accgtacgct cgctgctcga ccggtcgacg ttcgcgcgga
     4561 acctcggccg ggtcgccgcc cgcatcgacg ccccgccccc gcccgtcgac gaacccgact
     4621 tcggctgggt gttcgcgccc cgcatgccgg cctgggcgac ccccgacgcc gtggccgcgg
     4681 tccgtgccct gctcaacgac gccgccaccg agggacccgg gcccctggac gccgaccggg
     4741 cccggcacca ggcgctcgcc tcactcgtct tcgagggaac caccgtccgc caggtcaaca
     4801 ccgccctcgg ggacaccggc atcacctggg acgcgccctt cctcgacgac cgggtggtgg
     4861 aggcggccct ggccacccgg atcgaccagc gcctgctcgg agggcggttc aagctgctgc
     4921 tcacctcggc cgcggaaggg ctcgtccccg cggacatcct ggcccgccgc gacaagggcg
     4981 agttcagcgc ggaggcgttc cggggcctgg cccgcaaccg ggcccggatc ctggagctgt
     5041 gcgaggactc ccagctcgcc cggctcggcc tcatcgaccc ggcggccttc cggtccgcgg
     5101 tgctgaaccc ggggccgata tcccatcacc ttcagccgat cgacaccacc gtggcctgcg
     5161 agagctggct gcggacccac ccggagacac accccctgcc acccgcccgg aacacgccca
     5221 cgggagaact ccgatgaagc tgaccctcgc ccgcgacgtc accctcaccg tcgtcgacac
     5281 cggggccgta ctgctcgacg ggcgccgcgg ccgctactgg cagttgaacc actccggcgc
     5341 gggcgtcctg cgtcaactcc tcgacggaca ggcgcccgac gcggccgccg ccggcctgtg
     5401 cgccgcggcc ccggtcagcg acgaccaggc acggcaggac gtccaggccc tcatcgacgc
     5461 gctcagcgcg gccaagctcg tggaggtggc ctcgtgacca cccccgccgt ggccgaacag
     5521 gccccgcggc tgccctggta ccggcagctc gccccccggt gcgccgccgg ggcggcacgt
     5581 ctgctggtcc ggttgccgcc ggcccggctg caccgcgtgc tgggcgtggt cagcaagggg
     5641 tcccgccccg ccggatacgc cgaggtggcg cgggcccgcc ggtccgtcgt ctcggtcagc
     5701 acccgctgcg cgggcctcgg ttgcctccag cgttccgtgg ccaccgtcct gctgtgccgg
     5761 gcacacggca ggtgggccga ctggtgcacg ggattcagaa ccgaaccgtt cggcgcgcac
     5821 gcctgggtgg aggccgacgg gcggccggtg gacgagcccg gcgaactcag cgtgttccgc
     5881 acggtcctgg cggtccgtcg cccggacgaa cgccggagca cccctgaaag gagtcactca
     5941 tgacagcgat ccgggccgag ggcctctacg cgtactacgg caccacaccg gcgtgaacgg
     6001 gctcgacctg accgtgccca cgggcagcat gtacggcttt ctcggaccga acggcgcggg
     6061 caagaccacc accatcaaca tgctgaccac cctgctgcgg cccaccgcgg gccgcgcgga
     6121 ggtggcgggc ttcgacgtcg ccgcccggcc cgccgaggtc cgccgccgta tcggcatcgt
     6181 gttccaggag tcgaccctcg acctggacct caccgccgcc gagaacctcc gcttccaggc
     6241 cgacctgtgc ggcctgtccc gccgcgcgtc ccgtgacgcg atcgcctcga tgctcgacct
     6301 gatggacctc tccgagcgtc gcagggtgcc ggtacggcag ttctccaccg gactgcgccg
     6361 ccgcctggag atcgcccgcg gcctgctcgc cgagcccagc gtgctgttcc tcgacgagcc
     6421 gacgaccgga ctggacgccc agacccgcgc cgccgtctgg gagcacctgg agcggctgcg
     6481 gcgggagcgg ggcatcacgg ttttcgtcac cacccatcaa ctggacgagg ccgagcactg
     6541 cgaccggatc gcgatcatcg accggggcaa ggtggtcacg gagggcacgc ccgcggacct
     6601 caagtccgtc atcggtgccg agctcgtcgt cctgcgcacc gacgacgatc agcgcgccgc
     6661 cgaagccctc ggcgaccggt tcggtctccc ggcggagccc actccggacg gcctgctgct
     6721 ccgggtcgac cgcgcggcag ccctggtgcc ccgcctgtgc accgaactcg gcgtgaccgt
     6781 acgcgaggtc gccatcgccc cgcccacgct cgacgacgtc ttcctgcacc acaccggtct
     6841 cgccatccgg gagagcccga ccggcccgcg catgctcggc aacctcgggg aaggactgcg
     6901 atgagccgga ccgacaccgc acccgccgca ctcggcgaca tcaccgccgc tccaccaccg
     6961 gccgaccggt cccgcaaccc ggcacgcccc gtcctgctgc tctggcggcg ggagatgacc
     7021 cgactgaggc acaaccccgt acgcctggcc atgggcctgg tgacacccct gctgttcctc
     7081 gtcgtcctcg gcaccggcct cgacgcggcc tcgtccagcc tcggcaaagc ccaactgaac
     7141 gactaccggg cctacttgtt ccccggcacg ctggtcatgt ccgtgcaggc tccggcgatc
     7201 gcggtgggca tctcgctggt gtgggaccgc aggctggggg tgctgcgcca gatgctcgtg
     7261 gcgccgttcc cgcgcgcgtc catcgtgttc ggactggccc tcggcggcgc caccacgggc
     7321 gcggtctacg gtctcatggt gctgtccgtc ggcgggatcg cgagcatccg ctacacgccg
     7381 atgctgctgg tcgtcctgct cgaactcctg ctggtctccc tcatgttcac ctcgctcggg
     7441 ctgctcgccg ccgtcaccat ccggcaggtc gacaccttcc aggtcgtggt gaacctgagc
     7501 ctgatgccgc tgatgttctt ctccggcgcg atgttcccgc ccaacggcct gcccggctgg
     7561 ctcgacaccg tcgtcaagct caacccgctg acgtacggcg tcgacgcggt ccgccggacc
     7621 ctgccaggac cgagcgtgct cacctcggag cagacccggt tgatgctcgg cgactggcac
     7681 ccacccgtgg ccgccgaact gggtgtgctg gccgccctca ccgcggtcgc gctgggcctc
     7741 gcgggctacc ggttctcccg tacgtcatga gccggggagg acagggcacg gtggacgcga
     7801 gcgcgacgga cgcgggcacg acggatgtca cgaggagggc gaacacccgg gccacgaccg
     7861 ggtattgggc gggcgcggca cggctcgcgg cccggctgct cctggcggcg gtcctggcct
     7921 acgccggtct ggtgaagata ggggacctca cggaggccgg acggacggtc gcgctctacc
     7981 gcatcgtgcc cgacgactcc gcgcaactcg cgggtggcgt cctgccgttc gtcgaggtgg
     8041 cgctcgcgct gctgctcgtg gccgggctgg ccaccagggc ggcagcggcg ggcgcggccg
     8101 tactgctggt cgcgtatgtg gcggccatcg cctcggtgtg ggcacgggga atgtccatcg
     8161 actgcggctg tttcggcggc ggaggcacgc tcagcggtgg cgccgcacgc ggctacgcgc
     8221 tcgacatcgc gcgcgacctg gtgctgctcg gcgcggccgc cctcctgatc cggaatccgc
     8281 gcacccgata cgcgctggac agctggatcc tggacccgaa ggagtgaggg gcatgacgag
     8341 cacacagaca acggacgcca cggtgcggga gatggtgcac cgaaggcacc ggcgacggcg
     8401 cacggtggtg gtgtcgctgg tggccgccct ggtggtggtc gccgccgcgc tggtcggcgc
     8461 gggcctggtc cgggcgaaca acacggcccc cggcaaggca ccgagtcgcg taccggccgg
     8521 gatcgccgcc gacaagtcgg gcgtggccgc ctccaccggc gccgtacgcg tcgacgtgta
     8581 cctcgactac ctctgccccg aatgccgtcg taccgaacgg gccctgacca ccgccctgga
     8641 cgatctgagg tcgcacggcg gggtgagcgt cgtctaccac ccggtcgcct tcctcgacgg
     8701 ccgcagcgaa cccgcgggct actcgacaca ggcggcctcc gcggcggacg cggggaggtt
     8761 cgagcagtac tccacggtcc tgttctcgaa gcagcccgcc gaacagggcc cagggctcag
     8821 cgaggcccag ctgatcgcgg cgggccggga cgccggcatc accgcggcgt ccttcgcccg
     8881 ctgcgtcgag gacgccccgt accggccctg ggtacggtac gtctccgata tcgccgcctc
     8941 ccgcaaggtg gcgctgaccc cgaccgtgat ggtggcgggc cgccgcgtcg acgtcaccgg
     9001 ctccgatccg ggcggcgcgc tgacccgcgc ggtcacggag gcccggcggt gacccggctg
     9061 accgtggccc tgtccggcga ctgcatggcg acccggggag cggtgatctc ctccgacccg
     9121 gccgccggac ggctccgcga actcctccac ggcgccgact tcgccgtcac caacctggag
     9181 gtggtgtcca gcgacggacg cgggcacccg gtgcacaacg cggccggcgg cggctgtctg
     9241 atcgcggact ccggtgtcct ggacgagatc acggcggccg ggttcaccgt gctgggctgc
     9301 gccaacaacc acgccatgga cctgggcacg gagggtgtgc tcggcactgt ggaccaactc
     9361 cggtccaggc ggatcccgtt cgccgggatc ggtgcagacc tcaccacggc gcgccggccc
     9421 gtccacgtcg accggccggg cggcagcctg gcgctgctcg cctgcaccgc gacgttcctg
     9481 cccggccagg aggcggccga tccgtcgccg gaactacccg gacggccggg cctcaacccg
     9541 ctgcgccact ccgcgaccct gcgggtgacg gcccctcaga tggacgtgct gcggacgatc
     9601 gacgccgaga cgggcctgcg cgcccgccgg gccgaggccc gcaccctgct cggggtcgac
     9661 cccgccatcc tcgcgccgga cccgctcacc ctgttcggca cccgcttcct gacggcggac
     9721 gaaccgggct tcacgaccga gtgcgatccg cgcgacctcg acgagatctc ccgctgggtc
     9781 ggtgaggccc gcctccgcgc ggacctcgtg gtggcgagcg tgcactccca cgagcccagc
     9841 ccgacgcccg agacaccggg cgagttcctg cgtgccttcg cccaccggat gatcgacgag
     9901 ggcgcggacg tcgtggtcgg gcacggcccg catttccttc gcggcatcga gctgtaccga
     9961 aacaggccga tcttctacag cctcggcaac atcgtcagcc agatcgaact caccgaccgc
    10021 gtctccgccg aggactacgc caaggtcacc accgaccggg cgcccacccc tggccgctac
    10081 tacgaccggc tgagcggcca cggcacccgg ttgttcgccc cgcaccgccg ctactggcag
    10141 tcgctcctgc cggtcctcac cttcgacgac ggcaccctcg tgacggcccg cctccacccg
    10201 gtcgacctcg gcttcgccct gccggtgcac cgcaggggcc gcccccgtct ggccgaccag
    10261 gccgaggcca aagagatcct cactgatgtc acccggctgt cggagtcgta cggtacgacg
    10321 gtcggggcgg gggtcgacgg cacagcggag ctgcttctgg acgtggcttg aagggcccta
    10381 cgtggcgggc gagttgacga tagagccttg ctaaaatgtc atatgtcacc tacgagcgga
    10441 cgaggaaccg gggaacacgc atggccgagt cggtccgggt actgatcgcg gacgaccaag
    10501 cactgctgcg cgggagcttc cgggtcctcg tcgactccac acccggcatg gaggtcgtcg
    10561 gcgaggcggg cgacggcgcc acggcggtcc ggctcgcccg ggaactcgcc ccggacgtcg
    10621 tcctgatgga cctccggatg ccggttctcg acggcatcgc ggcgacccgg gagatctgct
    10681 ccgacgagac gctgaccggt gtccgggtgc tggccctgac catgttcgac atggacgagt
    10741 acgtctaccc ggccctccag gcgggcgcca gcggcttcat gctgaaggac gcctcgccgt
    10801 ccgacctggt cgacggcatc cgggtcatcg ccacgggcga gggcgtactg gccccgacgg
    10861 tcacccggcg cgtcatcgcc aacttctccc ggcgcggcga gacaccccgc gccgctccac
    10921 gactcgtcgg tctcaccaag cgggaacacg aagtcctcgt ccagatcgcc aacggcctgt
    10981 ccaacgcgga gatctccgaa cgcctggtca tcagcatgcc gaccgtgaag acccacgtcg
    11041 gcagcctcct ggccaagctg cacgcccggg accgcgcaca gctggtcatc atcgcgtacg
    11101 agagcggcct ggccgagcgg ggaatgccga cgtgaccgcg acgaccagga ctcacggcgc
    11161 gtcgccccgc cgggcggcgg cgcaggagtg gccgtcgtcg ccgacggcgg tgacgatcag
    11221 tatggcgccc cgcctccgcc attgggcact cgttgtactg gcaggtctgg ccctggcagc
    11281 aggtccatcg actcggtccc gccctgggtc ttcgccaagc tgatcacccg gcagaagggc
    11341 cacgtattgg ccccgggcgc aagcctgagc tatgtgaacg gccctggcag ccagctcctt
    11401 tggggcgtcc tccttgtccg tggtcagctg tcccgccagg ttgtagagag tgtctgcaac
    11461 acgacgcgtg tacggctcca tggtgaggcg caagcgccgg cgagcagtgc ggtccagacc
    11521 gcggcgagca gcgcgtcgac cagagaggca gccgcgaaga ggaccccgag cccaattgtg
    11581 atcggcaagg taccggacgg gcgcccgtgg agaattcgga gcccctgagg ttcccgcata
    11641 cccaatcccc tgaaccgcgt acgaaacctg ctctcccaga cactccccgt tgccgtcatg
    11701 gagatgaaga tggaggtggg tgttccaacg gccgatgccg accgtcaaga catgcccgaa
    11761 gggatggcgg actcgccaca tagtcccgcg ccaaccgagc agggctggtg tgcggctctc
    11821 cggaccaggc ggctccgacg gctagtgggc cacagcccgc acacccaggt gcctcacacg
    11881 gaacggattc agcacacacg aggccgggaa acgccgtagg tgcccttttg catacaggcc
    11941 tgacgaccgg ctcacccggt ggatacgaca cggccgccag gggcccggcg gatgtcgacc
    12001 caggccgcga aggctgcttc caccaaggca ccccagaatc acgacgacac cctgctaccg
    12061 atcgaccgga tcgccgcgga ccgcaacggt cccccgaaca gaggcccccc gtcgttactg
    12121 aagccattgg gccgttcgaa ggagttgagg cagcgagaca gccaagagcg tcacggaccg
    12181 tcagctgctt ttcttcctgc tctcagcgcg attccaagcc aggacagggc cgacacccgg
    12241 gcccctcccc agcgtcaatg gcccatacaa gggagagtta cggatatatc tgcactttgt
    12301 aacgcgaatc gtttccttgg tctggtgtcg cagtggaggg ccactgtgtg ggcctcggca
    12361 ccgagccggg aattacggat tgcacgcccg gccccgacca gggtcggtgt gcacgaacgt
    12421 gaggtgacag attcatgtct gtttctcgta cggcggtccg cctgatcgcc acctgtgtgg
    12481 cttcgggcgc actcgtcggc gcggccgcca tgccggccct ggccgccgga ggcggccacg
    12541 accgcgacaa cgggaggggt tactcgcaca gtcacaggag cgaccacgac cacgacggcc
    12601 acttcggcga ccgcgaccgg gaccggggct ggggctggga ccggggccac gaccggggct
    12661 gggaccgggg ccacgaccgg gaccggggct gggaccgggg ccgcgaccgg gatcaggacc
    12721 ggggcgggcg ccacggctgg tacagcgacc ggcaccggga tcaggaccgc taccccggcc
    12781 accgctagcc cgccttggag gctgtgctgt gacacggagg cccacaccct ctgccacccg
    12841 tcgtcatagc cggcacggcc tccataccca gccggagggc cgaagaccgc tccttgcagt
    12901 cttcggccct ccggccacgt gtcgcaccgt gcggtcgttg cccgggcacc gcaccaccaa
    12961 cgtccagtag cggccggcct tccagggaag cagcgcgacg atacggccgt cagagaccga
    13021 tccgtgtaga gctacgggtc agaaggcttc tgacatccac ggctggcatc aacgaggccg
    13081 gacggagggc gtacaccagc atctccgtcc ggctgtcgca ccagtcgacg gcagcagccg
    13141 gagcggaccc ggatcgaact cctagagcgg gtgtcgcagg ttcgaatcct gccaggtgca
    13201 cagcaagaag ggccggctca ggaggggttt cctccagggc tggtccttgg ccgtttccgg
    13261 tgccatgcca ctgcgtccga gtgacatcct tgttgttgct gtccgagggg ccacccccgc
    13321 cgtcgcgagt catcgcgccg aggcggtcgg caatggcccg atcacggtcg ctggtcacgc
    13381 gctgatggat cagcgcggcc cgcgaggacg actcagatat acgtctacag gacttgatcc
    13441 gagcgcgacc aatgcggtga aaaatgcagt cgccgcgctc gctggttgtc ttcatttgaa
    13501 gggcgtgtgc tggaatgtcg gcgacctgcc tatcggggtg catggccaat ggtctgggct
    13561 gcgccatttc ttcccatgtc cgctgttgct tgtacgccgt ggtgattgga aaagagatgg
    13621 tggaaatttc cgcggttaag ctttcctggc gcacgtgcac tacgccggtg tccctggtag
    13681 cacttgcgat cgcgagtgct ccgctggcct ctcaagccca ggccctggtg atgccgctgg
    13741 gtgtgaacgc ccgccatgca cctggcactc cgggggcccc acaggccccg gttgcggtgt
    13801 tcgccgaaga cttcgaaaac gcaggcgaga ctccgatctt cctggaggac tacgtcggcg
    13861 ctccaccgct caacgagaca tacacagccg accccccgtg gcttgaccac ggacagtgca
    13921 acggcatcat cctcgatcag accggtgctg atcagcctga ctgcccggca gtattgaaga
    13981 acatggccaa tgcgctggga caggtaggcg gaacgaatcc gccgaccaac cacgtggtcg
    14041 cggcctacac caactgggtg ccacctggcg ccgaccgggt ggagttccgg accgagcggc
    14101 cgatcccgat cgcgaagccg aaccgctata tcaccttcgc agtggatgta gccgccgtga
    14161 attgcggcca agccgctcct ccgctgctga agttctatct gaccggcaac gggtcagaca
    14221 tcccaacctt caccaccccc atcaatccct gcgccgaccc caactccaag ccctatcccg
    14281 gaggaaacgg gttgagggcc ggggcgttcg ccggcaacag agctgtactg ttcaacgata
    14341 gccagttggg catcaagatg gtcaatgggc aaggcgaatg gttcggtaat gaccacgcat
    14401 tcgacaacat ccggatcctg gatgcgactc cgcaactgga caaggcgttc agtcctgcca
    14461 ccgtgaacaa gggtggcacg tcgacgctca ccatgacggt gacgaacacc agtgaactgg
    14521 cggcgaagaa cgacttcagc ttcgtcgaca acctgcccgc cggagtgaag gtggccgcga
    14581 acgcgaacgc gtcgacgacc tgcggcaacg gcacggtgtc cgcggcggcg gggggcgcgt
    14641 ccgttgctct gaacggcggc gacctggccg ccggcgagaa gtcctgcacg gtcacggtga
    14701 acgtcacagc cgacaaggcg ggaacctacg tgaaccgccc cgaagcgatc accacggtgg
    14761 gcctgaatcc cccggacccc gcgaccctga ccgtcaggaa aaagggagca acagcggtgg
    14821 gcaccgcgac gggctcgggc ggtctgctgt ccggcaatgt ggtgcaggta ccagtggaca
    14881 tgccggtcaa cgcttgcggc aactccgtga acgtcatcgg gctgctcaac ccggcgacgg
    14941 gcaacgtctg cgtcaacggc tagccgccca ggtcactcgc cgcgccggga ccgggcaggt
    15001 cccggcgggc acagatcgtc cggctgacat catcagttga caccaacgcc gctgaacgag
    15061 ggcagacaac ggcaactccg cccgcagtgg cagacagcca taagggcccg ttccgtgagg
    15121 tcgccccaca cagcgaccga actcccaaag cgggtgccgc aggttcgaat cccgccggaa
    15181 gcacgcggtc cgaccaggcc atatgtatcg aacagccccc atggagcgat ctccgagggg
    15241 gctgatttcg tgtgctcggg gacaatgcgg gacgtaacgc caggcgacgt cctgatcacg
    15301 gtttttaccg tccccgctcg catcggcctt gaccaggtac agcttcgacg tctcccccga
    15361 cttcacggtc gaagtcgttt cggcccgtac ccacagtggt gtgcgaaccg ggtcaaccgt
    15421 gttcacccaa ggcctgccgc cccgaagccg cgcactcatc cagcttgtcc cccgctactt
    15481 ctcgccaatc catccgctcc atggctcggc gtcgcgatcg agtaattcgc cgcgccgtaa
    15541 gccgacctcg cccggagctg accagctcag actctcgatg gaggacacag cctccagccc
    15601 tcaagctttg ctcaattccc tggtcctgat tggagaactc atgcgaacat cgcgtggtct
    15661 gctgccatcc gaccaggtgg cgggcatgat tcagggggaa tctccatgct cttttcgccg
    15721 aagtcgccga cgtaccgcca tgaccatcgt gcactggcag ccgtcggcgc ggcgcccgtt
    15781 tcatcgatcc gggctttgtc gtgcgaagcg gtgcaacgtc cggctcggct gggacaccac
    15841 tgtcaagtct gatcgaacct acccatgttc ccaggtggtt ctgaccagca agaccgctga
    15901 aggccccacg aaatatggcc caactctgcg ccgtgggagc cag
//
