LOCUS       NZ_BMUQ01000007        10798 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces mirabilis strain JCM 4551 sequence07, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUQ01000007
VERSION     NZ_BMUQ01000007.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces mirabilis
  ORGANISM  Streptomyces mirabilis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 410989)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 117X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650275.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:52:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,392
            CDSs (total)                      :: 10,311
            Genes (coding)                    :: 9,898
            CDSs (with protein)               :: 9,898
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 413
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 172 of 414
            Pseudo Genes (incomplete)         :: 329 of 414
            Pseudo Genes (internal stop)      :: 40 of 414
            Pseudo Genes (multiple problems)  :: 115 of 414
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:21:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 69744
            Orig. end                         :: 80542
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUQ01000007.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10798
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="1"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10798
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10798
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5798](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5798
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            179..496
                     /locus_tag="IE289_RS19295"
                     /old_locus_tag="GCM10010317_039720"
     CDS             179..496
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020554869.1"
                     /locus_tag="IE289_RS19295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_039720"
                     /product="putative quinol monooxygenase"
                     /protein_id="WP_190170449.1"
                     /transl_table=11
                     /translation="MIVNHGFNATLTARPGMGDQLADLLLTGLSDGNPGTSEHCLVYLV
                     CRSASNPDVVHVTEGWTSEEDHHRVFAGEAAQAIVKRLDGLLAKDPEYTDYVPVHGKAA
                     F"
     gene            590..1558
                     /locus_tag="IE289_RS19300"
                     /old_locus_tag="GCM10010317_039730"
     CDS             590..1558
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1066: alpha/beta hydrolase domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056590.1"
                     /locus_tag="IE289_RS19300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_039730"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_190170450.1"
                     /transl_table=11
                     /translation="MTLARPALDPELAALLADMPLMTHLGPELLAQLRLLPSTPVEPLL
                     EGRSVDRREVSVSGEDGARIPLSVFSPAGTAHAAGAPCVFWIHGGGMVMGDRFSQIDIP
                     LEWLDLFGAVVVSVDYRLAPEVSGTTLVDDCYQGLLWIAEHADELGIDPARIVVAGASA
                     GGGLAAGVTLMARDGGTPPIAAQVLICPMLDHRNITTSSRQYSGDPGVWTREMNEFGWR
                     SVLGDLRDERVPGHVSPAVADDLSGLPTTYVDAGSAEVFRDEDVAYATRIWAAGGQAEL
                     HVWAGGFHGFDALYPQARVSAEARRTRNDWLARVLSPASVA"
     misc_feature    1612..1629
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.5"
                     /tool="antismash"
     gene            1684..2442
                     /locus_tag="IE289_RS19305"
                     /old_locus_tag="GCM10010317_039740"
     CDS             1684..2442
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (2-170). E-value: 3.8e-09. Score:
                     28.6. Matches aSDomain:
                     nrpspksdomains_IE289_RS19305_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545211.1"
                     /locus_tag="IE289_RS19305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_039740"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_190170451.1"
                     /sec_met_domain="adh_short_C2 (E-value: 8.6e-32, bitscore:
                     105.5, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVAIITGASSGIGQSAAIQIAKRGTGVILTYGNNAQGGLETAAA
                     IEKDGGTAVALPLDVGETGAFPAFRRSVVDVLRDTWQRDTFDYLVNNAGFAHTSLIEDT
                     TEEAFDRLMRVLLKGPYFLTQRLLPLMEDGGAIVNTSSNSAMGSGLEPGYSAYASMKGG
                     LNVLSRYMAKEFSARGIRVNSISPGSTRTRIADDAFERFPEVIPALAAKTALGRVGEPD
                     DIGMMIAALLSEESRWVTAQNIEVSGGYNL"
     aSDomain        1690..2193
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE289_RS19305_PKS_KR.1"
                     /evalue="3.80E-09"
                     /label="IE289_RS19305_PKS_KR.1"
                     /locus_tag="IE289_RS19305"
                     /protein_end="170"
                     /protein_start="2"
                     /score="28.6"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VAIITGASSGIGQSAAIQIAKRGTGVILTYGNNAQGGLETAAAIE
                     KDGGTAVALPLDVGETGAFPAFRRSVVDVLRDTWQRDTFDYLVNNAGFAHTSLIEDTTE
                     EAFDRLMRVLLKGPYFLTQRLLPLMEDGGAIVNTSSNSAMGSGLEPGYSAYASMKGGLN
                     VLSRY"
     CDS_motif       1696..1764
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE289_RS19305_0001"
                     /evalue="8.50E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE289_RS19305"
                     /protein_end="27"
                     /protein_start="4"
                     /score="12.0"
                     /tool="antismash"
                     /translation="IITGASSGIGQSAAIQIAKRGTG"
     gene            2538..3717
                     /locus_tag="IE289_RS19310"
                     /old_locus_tag="GCM10010317_039750"
                     /pseudo=""
     CDS             2538..3717
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009327274.1"
                     /locus_tag="IE289_RS19310"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_039750"
                     /product="SMI1/KNR4 family protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MPSLHDFATWEPVLRLLRTADAESIAAPGGYVAGRIGQHGSSLPM
                     PRRRPQPGRALQIEDMQDEFDAVERVRGALADTGVDDISFAAEISRDGSAVLHLLHPSP
                     AAEPGIGNAHPGSLILVEDAVPEPWRRLPEPVPAAVPAPSVDLASLERTLRERIPDAIG
                     ATEAEIAGAEARLGVVLPDELKVLYRVTRARWEDWGDDHEAAERVSDAVGCELFSLDEL
                     YIADAPSRPSSWKFAAMKAVGTPPDAAVQGLVGSPAGSSSATTAAATASRWT"
     misc_feature    3745..3760
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.08"
                     /tool="antismash"
     gene            3995..4978
                     /locus_tag="IE289_RS19315"
                     /old_locus_tag="GCM10010317_039760"
     CDS             3995..4978
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018566630.1"
                     /locus_tag="IE289_RS19315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_039760"
                     /product="Dyp-type peroxidase"
                     /protein_id="WP_190170452.1"
                     /transl_table=11
                     /translation="MVESQFVITPPAKAALFLVCTMAPGGEPAVRDLLQDVAGLRRSVS
                     FRAPDAELSCVVGIGSAAWDRLFAGPRPHDLHPFVALTGSRHRAPSTPGDLLFHLRAHH
                     MDLCFELAQLIGERLYGAVAVVDEVHGFKYFDERDLLGFVDGSENPEGQVAVDAVFIGD
                     EDPDFAGGSYVIVQKYVHDLAAWNALPTQEQERVIGRTKLANVEMPDDIKPADSHVALN
                     TITDADGNERKIVRDNMPFGSIGQREFGTYFIGYARTPEVTEQMLRNMFLGDHPHTHDR
                     ILDFSTAVTGCLFYVPSADFLDDPPAAPTGASATEGSLGIGSLKGA"
     misc_feature    4995..5013
                     /note="TFBS match to LexA_variant_3, Repressor of DNA
                     damage response, confidence: weak, score: 20.86"
                     /tool="antismash"
     gene            5001..5798
                     /locus_tag="IE289_RS19320"
                     /old_locus_tag="GCM10010317_039770"
     CDS             5001..5798
                     /GO_component="GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: Linocin_M18"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014135064.1"
                     /locus_tag="IE289_RS19320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_039770"
                     /product="family 1 encapsulin nanocompartment shell
                     protein"
                     /protein_id="WP_190170667.1"
                     /sec_met_domain="Linocin_M18 (E-value: 2.1e-89, bitscore:
                     293.5, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNNLHRELAPITPEAWAQIEEEARRTFQRNVGGRRVVDVTGPDGP
                     ALASVGTGHLTRIEGPGPGVTALLRQVQPLVELRVPFKVGRDAVDDVERGSNDSDWQPV
                     KDAARAMAFTEDRAVFEGYEAAGIDGLRRRTSHPVHGLPAETRDYPDAVSRALTTLRLA
                     GVAGPYSLLLGADAYTLVSETSDHGYPITEHLSRMLDAPPIWAPAVDGAFLLSTRGGDF
                     ELRLGEDLAIGYTAHDANEIELYFRQSLTFLVYTDEAVVALGS"
     gene            complement(5852..5995)
                     /locus_tag="IE289_RS19325"
                     /old_locus_tag="GCM10010317_039780"
     CDS             complement(5852..5995)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE289_RS19325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010317_039780"
                     /product="hypothetical protein"
                     /protein_id="WP_190170453.1"
                     /transl_table=11
                     /translation="MTSEAGAISAEARRALEQELADLHVERGAVAATLGGATVTVRSLG
                     AP"
     gene            6169..6549
                     /locus_tag="IE289_RS19330"
                     /old_locus_tag="GCM10010317_039790"
     CDS             6169..6549
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676862.1"
                     /locus_tag="IE289_RS19330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_039790"
                     /product="NUDIX hydrolase"
                     /protein_id="WP_099925129.1"
                     /transl_table=11
                     /translation="MTLEQAARGVVDAVVVYDGRLLMVAAQDSWGLPSGAPQPAESAQA
                     GAARLVYELTGYLVDGTSVLRPQGTAGEDARPAVVCQLLSQAPSAEARLGPEQIRWAPF
                     AEAIDAGLPEAVRDYLVGHTPV"
     gene            6673..6978
                     /locus_tag="IE289_RS19335"
                     /old_locus_tag="GCM10010317_039800"
     CDS             6673..6978
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE289_RS19335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010317_039800"
                     /product="hypothetical protein"
                     /protein_id="WP_190170454.1"
                     /transl_table=11
                     /translation="MQIITITAAVIVGALSSITYVQYAASRSPGRPIPLFSTRLALFPL
                     WADVTFTTGWIVSSFTLIFMLPIWMGVAACCGATVLPFEVMRRRHNRQVRGRVLTP"
     misc_feature    7109..7124
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.11"
                     /tool="antismash"
     gene            7176..7802
                     /locus_tag="IE289_RS19340"
                     /old_locus_tag="GCM10010317_039810"
     CDS             7176..7802
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003952221.1"
                     /locus_tag="IE289_RS19340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_039810"
                     /product="DUF1345 domain-containing protein"
                     /protein_id="WP_229889046.1"
                     /transl_table=11
                     /translation="MSIRLSLSAVPRLAIAVAVGAAVGVAVALSSDTPLAILAGIAATQ
                     TFFVVVGWIVLWPMDAASTRRNARREDLRPVLEEIVVVTVALCGLVGIVVFLLGNSDSG
                     HAATALLGVFMAWAALHLMYATRYAYLYYVDSGGGIDFNSDQPPAYRDFFYFSYNLGMT
                     YQVSDTDVSSAVIRSMVLRHTLLSYVFGTSILATAINLFAGILVH"
     gene            7882..9615
                     /locus_tag="IE289_RS19345"
                     /old_locus_tag="GCM10010317_039820"
     CDS             7882..9615
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018548760.1"
                     /locus_tag="IE289_RS19345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_039820"
                     /product="FUSC family protein"
                     /protein_id="WP_190170455.1"
                     /transl_table=11
                     /translation="MSRHHRTKLTRVLSPRGALTLHAVDGALLFALRAALAMALPAVPL
                     VLSGRTALAVFPMLGAFTTTFGRNLPYRRRARVLAVAAVAMTMCVGLGATLAALTDPQA
                     GGLGAAVVIAAMAGTAGLAKFLCDATRLSGLGAVLMLFSFAVAANGPAGRVTDVLAQTG
                     LAATGAATAWALALVGWVVHPDRPQRLAVAVALRELADLLEAGGGADETGHTRHRATAA
                     VLRAYQSLGLSPLTTDQRHGERSGTCVFLTDLAWSLLITSARRQTPDAPTARYLRVQAR
                     YLTVRYRRPPAPLPHPMFPSAPSALSAPSADTAPGHPDEAGPAARRAAELTVGTGAGSP
                     GHVAVLVVPAFRMALGTGLAGGLAAFLSFGHGYWAALSAAAVLHSVNVRSTLERALQRT
                     LGTAAGLLIAFGVLATHPAPTALVALIVVFEFLLEYTVARNYGLGVVFLTPLALLMSDL
                     ASPAPVGTLVHDRTLGSLLGILIGLACALLVVHGRPAARVERALAACGEASERAEQALA
                     DSAAVPHPAVQTQLAVAVVELREADDAAAGELYAADVDPAQLAAAEQRAYVLLERLHRT
                     T"
     misc_feature    9715..9731
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 17.97"
                     /tool="antismash"
ORIGIN
        1 ccctgtcgtc gactcctgca cggaagagga ttgtgcaagg agccgacagc atcgttctac
       61 cgtttccgca ggtcaggccg cttgaatgga gtcactggag tccagcggag agccaaaggg
      121 ccctccgctc caggaccagc acacctgcac gatccgtcca tcgcggaaga aggtagtcat
      181 gatcgtcaat cacggattca acgccaccct gaccgccagg cccggcatgg gcgaccagct
      241 ggccgacctg ctgctgaccg gcctgagcga cggcaacccc ggcacgagtg aacactgcct
      301 cgtctatctg gtctgccgtt cggcgtccaa ccccgacgtc gtccatgtca ccgagggctg
      361 gaccagcgag gaggaccacc accgggtctt cgctggcgaa gccgctcagg ccatcgtgaa
      421 gcggctcgat ggactgctgg cgaaggaccc cgagtacacc gactacgtac cggttcacgg
      481 caaggccgcc ttctgaggcc gctgttggat ccccagacct tccccacgcc ggtgggcagg
      541 cgccctccac ttctgaccat cccgtacgac ccgacgaaag gtagtcaccg tgactctcgc
      601 acgacccgcc ttggaccccg aactggctgc gctgctcgcc gacatgccgc tcatgaccca
      661 cctcggcccc gaactgctcg cgcagttgcg tctgcttccg tcgacaccgg tcgagcccct
      721 tctcgaaggc cgctcggtcg accggcgcga ggtcagcgtg tcaggcgagg acggtgcgcg
      781 gattcccctg tcggtcttca gccccgccgg gaccgcgcac gccgcggggg cgccgtgcgt
      841 cttctggata cacggcggtg ggatggtcat gggagatcgc ttctcacaga tcgacatccc
      901 tctggagtgg ctcgacctgt tcggtgccgt cgtggtctcc gtggactacc gattggcgcc
      961 cgaagtcagc ggcacgactc tggtcgacga ctgctaccag gggctgctgt ggatcgccga
     1021 gcacgccgac gagttgggca tcgaccccgc ccgcatcgtc gtggcgggcg ccagcgccgg
     1081 cggtggcctc gccgccgggg tcaccctgat ggcacgcgac ggcggcaccc cgccgatcgc
     1141 cgcccaggtg ctcatctgcc ccatgctcga ccaccgcaac atcaccacct cgagccggca
     1201 gtattccggc gatcccggtg tctggactcg tgagatgaac gagttcggct ggcgctcggt
     1261 gctcggcgac ctccgggacg agcgggtgcc cggccatgtc tcccccgcgg tggccgacga
     1321 cctctcgggc ctgccgacca cctacgtcga cgccggctcg gccgaggtct tccgcgacga
     1381 ggatgtcgcc tacgccaccc ggatctgggc ggccggcggc caggcggaac tccacgtctg
     1441 ggcgggaggc ttccacggtt tcgacgccct gtacccgcag gcgcgtgtct cggccgaggc
     1501 ccgccgcaca cgcaacgact ggctcgcccg agtcctgtcc ccagcctctg tcgcatagcg
     1561 gagccggcga cgccacaccg gccgactacc gcgccagtca cggcatttcc catgtgacac
     1621 ccgtgttgcc gaggcccgcg ccgggcctcg gcagacgctc aaccacaccc caggagaacc
     1681 atcatgaagg tagcaatcat cactggcgcc agctccggca tcggacagag cgcggcgatc
     1741 cagatcgcca agcgaggaac cggagtcatt ctgacctacg gcaacaatgc ccagggaggg
     1801 ttggagacgg cggccgcgat cgagaaggac ggcggcactg ccgtcgcgct gccgctggac
     1861 gtcggtgaga ccggcgcctt cccggccttc cgccggtccg tggtcgacgt gttgcgcgac
     1921 acctggcagc gcgacacgtt cgactacctg gtcaacaacg ccggcttcgc acacacgtcg
     1981 ctcatcgagg atacgaccga ggaggcgttc gacaggctga tgcgggttct gctcaaggga
     2041 ccgtacttcc tgacgcaacg cctgctgcca ctgatggaag acggtggggc catcgtcaac
     2101 acgagcagca actcggcgat ggggtccggc ctggagcccg gatactcggc ctacgcgtcg
     2161 atgaagggcg gcctcaacgt gctgagccgc tacatggcca aggagttcag cgcgcgaggc
     2221 atccgcgtca attccatctc gccgggctcg acgcgcaccc ggatcgccga cgacgccttc
     2281 gagcggtttc ccgaggtcat cccggccctc gcggcgaaga ccgctctcgg ccgggtcggc
     2341 gaacccgatg acatcggcat gatgatcgcc gccctgctct ccgaggaaag ccgctgggtc
     2401 accgctcaga acatcgaggt gtcgggaggc tacaacttgt agccatgagc agcccgacaa
     2461 cggcctgcgc caccacacgg cccacccgta ctcgcgagag tgcgaggcca gttcaccaga
     2521 gcgaccggag cctgccgttg ccctcactac atgacttcgc gacctgggag cccgtgctgc
     2581 ggcttctgcg aaccgccgac gcggagagca tcgccgcacc gggcggctac gtggcgggac
     2641 ggatcggcca acacgggtcg agcctgccca tgccgcggcg gcgtccgcag cccggacgag
     2701 ccctccagat cgaggacatg caggacgagt tcgacgcggt ggagcgggtg cgcggcgcac
     2761 tcgcggacac gggtgtcgac gacatctcgt tcgcggcgga gatctcgcgc gacgggagtg
     2821 ccgtactcca cctgctccac cccagccccg ccgcggagcc cggtatcggc aatgcgcatc
     2881 cgggttcact catcctggtc gaggatgccg tccccgagcc ctggcgccgt cttcccgaac
     2941 cggtccccgc agcggtgccc gccccgtcgg tggatctggc gtcgctggag cggacgctcc
     3001 gcgagcggat ccccgatgcc atcggcgcca ccgaggcgga gatcgccggc gcggaagcgc
     3061 gcctcggcgt cgtgttgccc gacgagctga aggtgctcta ccgcgtgacc cgggcgcggt
     3121 gggaggactg gggcgacgac cacgaggcgg cggagcgcgt ctccgatgcg gtcggctgcg
     3181 agctcttttc tctggacgag ctgtacatcg cggacgctcc gtcccgtccg tcctcctgga
     3241 agttcgcggc gatgaaggcg gtcggcacgc cgcccgacgc cgcggtgcag gggttggtcg
     3301 gctccccggc tggatcgtct tcggcgacaa ctgcggcggc gaccgcatcg cggtggacct
     3361 gacaccgggc ccgcgcggac acacgggaca gatcatcatg gggagccgct cagcctcgcc
     3421 cccgtcatcg ggctcccccg cctgcggacc ctcacggcct accccggtac gctcgccgac
     3481 ccgctggaga tcgccgagct gaccgggctc gagttcccgg aactcgggcc ggaggattgg
     3541 cgtgtcctgc tcgatgccag agccgtcccg cgtagcacgt cggccgcctc catcgagatc
     3601 cacggcatcc gccacccgct cccgatcgtg gccctcgcca acgaactcct cgccctctgg
     3661 gaccgccctc cgatcactcg gacgactctc gaaggcaacc tcggccccgt ggcgtagacc
     3721 ggttcgcccc ggcgggtcct cgcctcgaag tcctgtccgc cacaaccctt tcacgaccct
     3781 tttcgcggat cgccaggatt ccctcgtgaa gaaggcgcaa tggatggggc gctaatcctc
     3841 gaccaccagg ggtcgggtgc gattcgcgca ggcaggcgcc tgtgtacccg caccggtcga
     3901 catcgccgta acccaatcct agtatttatt ttgtttcctc acgttatcct gccgtgcgta
     3961 gcgccatagc cacgacaagt aggaggcagg ctccgtggtt gagtcgcagt tcgtgatcac
     4021 cccgcccgcc aaagccgccc tgttcctcgt gtgcaccatg gcacctggcg gcgagcccgc
     4081 tgttcgcgat ctgctccagg atgtggccgg gttgcgacga tcagtcagtt tccgcgctcc
     4141 ggacgcggag ctgagctgcg tcgtcggcat cggctccgcc gcctgggacc ggctcttcgc
     4201 aggaccccgc ccacacgatc tgcatccgtt cgtcgcgttg acgggctccc ggcaccgagc
     4261 cccgtccacc ccaggtgacc tgctcttcca tctgcgagcc caccacatgg acctctgttt
     4321 cgaactggcc cagttgatcg gcgaacgcct ctacggagcg gtcgccgtgg tggacgaggt
     4381 gcacggtttc aagtacttcg acgagcgcga cctcctcggc ttcgtcgacg gcagcgagaa
     4441 ccccgagggc caggtcgcgg tcgacgcggt gttcatcggc gacgaggacc ccgatttcgc
     4501 gggcggcagc tacgtgatcg tgcagaagta cgtgcacgac cttgccgcct ggaacgcgtt
     4561 gcccacgcag gaacaggaaa gggtcatcgg ccgcacaaag ctggcgaacg tcgaaatgcc
     4621 ggacgacatc aagcccgcgg actcgcatgt cgcactgaac accatcaccg acgcggacgg
     4681 aaacgaacgg aagatcgtcc gtgacaacat gccgttcggc agcatcgggc aaagggagtt
     4741 cggtacctac ttcatcggct atgcgcgtac tcccgaggtg accgaacaga tgctgaggaa
     4801 catgttcctc ggtgaccacc cccataccca cgaccgaatc ctcgactttt ccacggcggt
     4861 caccggctgt ctgttctacg taccgagcgc cgatttcctc gacgacccac ccgccgcgcc
     4921 caccggagcg tcggcgaccg agggctccct ggggatcggc agtctcaaag gagcctgatc
     4981 acgtgtccac gcccaacggt atgaacaacc tgcaccgcga actcgcgccg atcacgcccg
     5041 aggcatgggc acagatcgag gaggaagccc gccgtacctt ccagcgcaac gtggggggcc
     5101 gtcgtgtcgt ggacgtcacc ggacccgacg gtcccgcgct tgcctcggtc ggcaccggcc
     5161 atctcacccg catcgaaggg cccggaccgg gcgtcaccgc tctcctgcgc caagtacagc
     5221 ccctggtcga actgcgcgtc cccttcaagg tcggtcgcga tgcggtggac gacgtagagc
     5281 gcggctcgaa cgactccgac tggcaaccgg tcaaggacgc ggcgcgcgcc atggcgttca
     5341 ccgaagaccg ggccgtcttc gaggggtacg aggcagcggg catcgacggg ctgcgcaggc
     5401 gcacctccca tccggtgcac ggtctgccgg cggagacacg tgattacccg gatgccgtca
     5461 gccgcgcgct gacgaccctg cgcctggccg gagtcgccgg cccgtattcg ctgctgctgg
     5521 gcgccgacgc gtacaccctg gtgagcgaga cgtccgacca cggctacccg atcaccgagc
     5581 atctcagccg gatgctggac gccccgccga tctgggcgcc cgcggtcgac ggcgcttttc
     5641 tgctgtccac ccgcggtggg gacttcgaac tgcggctcgg tgaggacctc gcgatcggct
     5701 acaccgcgca tgacgcgaac gagatcgagt tgtacttccg ccagagcctg accttcctgg
     5761 tgtacaccga cgaggctgtg gtggcactcg gctcgtgacc gcacggcgcc cgagcggacg
     5821 ggaattgctc cgggagacgg cgacacgggt cctacggggc gcccagggac cgcaccgtca
     5881 cggtcgcgcc gcccagggtt gccgcgaccg cgccgcgttc gacgtgcaga tccgcaagct
     5941 cctgttcgag cgcccgacgg gcttcggcgc tgatcgcccc ggcttcactg gtcattcctg
     6001 ctcccgttgt gcaggtggaa cagctgggtg aggatgggcg attccgctgt ttgcgaccat
     6061 ggtcgcgagc tcgccgcgcc gcgtgaaccg atgtgccccg ggtaggaaat gggggctcgg
     6121 acgcggccgg tcggccggtc gcgcgaggag gaaagggacg gttgggccat gacgctggag
     6181 caggctgccc ggggcgtggt ggacgcggtc gtcgtgtacg acgggcgtct gctgatggtg
     6241 gccgcgcagg acagctgggg gctgccgtcg ggcgccccgc agcccgccga gtcggcgcag
     6301 gccggcgcgg cgcgcctggt gtacgagctc accgggtacc tggtcgacgg cacatcggtg
     6361 ctgcggccgc aggggacggc tggagaggac gcacggcccg ctgtggtgtg ccagctgttg
     6421 agccaggccc cctcggcgga ggcgcgactc ggcccggaac agatccgctg ggctccgttc
     6481 gccgaagcca tcgacgcggg gctgcccgaa gccgtgcggg actacctggt gggccataca
     6541 cccgtgtagg gatgcgggcg cggcatttat caggatcggt tcctgatttc aagtcacgct
     6601 gttccgtgac atgacgcgaa gcccctcggt gaacttttgt ggatgcagaa tttctcaggt
     6661 caggagattc tcatgcagat catcaccatc acggccgccg tcatcgtcgg ggcattgtcc
     6721 agcatcacct atgtgcagta cgccgcgtca cgcagcccag gacgaccgat ccctctgttc
     6781 tccacccggc tcgcgctttt tcccctatgg gccgacgtga cgttcacaac gggctggatc
     6841 gtcagctcat tcacgctcat tttcatgctg ccgatctgga tgggagtggc cgcttgctgc
     6901 ggggccacgg tactcccctt cgaggtgatg cgccgccgtc acaaccgcca ggtcagaggc
     6961 cgtgtgctca cgccatgagc gcgatgccgg ataccaccga caccgcggtc gcggccccgc
     7021 caccggtaag gagttccggt cccgtaggag ctcgtgacac gatcacgacg cggggccgtc
     7081 ccgtaggccc ggctgtgggc gggataccgt caccctctgg tcccgctctc gccgtgccga
     7141 gcagccacga agcactaccg ttgtggcttg tgagcgtgag catccgtttg tcgctttccg
     7201 cagtcccacg gcttgccatc gcggtcgccg tcggagcggc ggtcggcgtg gccgttgcgc
     7261 tgtccagcga cacaccgctg gccattctcg cgggtatcgc cgcgacccag acattcttcg
     7321 tggtggtcgg ctggatcgtg ctgtggccga tggacgccgc ctcgacccga cgcaacgctc
     7381 gacgcgagga cctgcgcccc gtcctcgagg aaatcgtcgt cgtcaccgtc gctctgtgcg
     7441 gtctggtcgg catcgtggta ttcctgctcg gcaactccga cagcggccat gccgcgacgg
     7501 cgctgctcgg cgtgttcatg gcctgggcgg cactgcatct gatgtacgca acccgctacg
     7561 cgtacctcta ctacgtggat tccgggggcg ggatcgattt caactccgac cagccgccgg
     7621 cgtaccggga cttcttctac ttcagctaca acctcggcat gacctaccag gtctccgaca
     7681 ccgacgtctc cagtgcggtg atccgctcga tggtgctccg gcacacattg ctgtcgtacg
     7741 tcttcggcac cagcattctg gctaccgcca tcaacctctt cgcaggaatc ctcgttcact
     7801 gatctcgaag aagtgggcag atgtggcaat tccacgggtg gatcgcgcaa ttatcatggt
     7861 gttccggact gtctcgattc tatgtcccgc catcaccgca ccaagttgac gcgcgtcctc
     7921 tcgccgcgcg gcgcgctcac cctgcacgca gtggacggag cgctcctctt cgctctgcgc
     7981 gcggcgctgg ccatggcgct gcccgcggtg ccgttggtgc tttccggacg aaccgcgctc
     8041 gccgtctttc ccatgctggg cgcgttcacc accacgttcg ggcgcaacct gccctaccgc
     8101 cgtcgcgccc gcgtcctcgc cgtggccgcg gtggccatga cgatgtgcgt cggccttggc
     8161 gcgacgctcg ccgcgctgac cgatccgcaa gcgggaggtc tcggcgctgc cgtggtgatt
     8221 gccgcgatgg cgggcacggc cggcctggcg aagttcctct gcgacgcgac acggctgagt
     8281 ggcctcggtg ccgtgctgat gctcttctcg ttcgccgtgg cggccaacgg cccggccggc
     8341 agggtcaccg acgtgctcgc ccagacaggg ctcgccgcaa cgggcgcggc cacggcctgg
     8401 gcactggcac tggtgggctg ggtcgtgcac ccggaccgcc cgcagcggct cgcggtggcc
     8461 gtggccctcc gcgaactcgc cgacctgctg gaagcaggcg gaggagccga tgaaaccggg
     8521 cacacacggc accgggcaac cgccgccgtc ctccgggcgt atcagtcgct cggcctgagc
     8581 cccctgacaa ccgatcagcg gcacggcgag cggagcggga catgtgtctt cctgaccgac
     8641 ctcgcctggt cgctcctgat cacctccgca cgccgacaga ctcccgacgc cccgacagcg
     8701 cggtacctgc gtgtgcaggc tcggtacctg accgtcaggt accgccgtcc cccagccccg
     8761 ctgccgcacc cgatgttccc gtccgccccg tccgccctgt ccgccccgtc cgcagatacg
     8821 gcacccggcc atccggacga ggccggtccc gcggcccggc gcgccgccga actgaccgtg
     8881 gggaccggtg ccggcagccc gggccatgtc gcggtccttg tcgtccccgc gttccggatg
     8941 gctctcggca ccggcctggc aggtggcctg gccgcgttcc tgagctttgg acacggttac
     9001 tgggccgcac tctccgcggc cgccgtcctg cattccgtca acgtccgctc caccttggag
     9061 cgtgccctcc aacgcacctt gggtacggcg gccgggctct tgatcgcgtt cggcgtgctg
     9121 gccacccacc cggcgccgac ggctctcgtg gccctgatcg tggtcttcga attcctgctg
     9181 gaatacaccg tggcccggaa ctacggcctc ggcgtcgtct tcctcacgcc gctcgcactg
     9241 ctgatgagcg acctggcatc ccccgcccct gtgggaacgc tcgtccacga ccggacgctg
     9301 ggcagtctcc tcggcatcct catcggtctg gcctgtgccc tgctggtggt ccacgggcgt
     9361 ccggcagcac gtgtggaacg agcgctggcc gcgtgcggcg aagcatccga acgcgccgaa
     9421 caggcactgg ccgactccgc cgccgtaccg caccctgccg ttcagacaca gctggccgtg
     9481 gcagtggtgg aactgcgcga ggcggacgac gccgccgccg gggagctgta cgcggcggat
     9541 gtcgaccccg cccaactggc agccgcggag cagcgcgcct atgtgctgct cgagcgcctt
     9601 catcggacca catgaatccc gcacatacga ccgacgcggg cgacccgcgg gcgccgcatg
     9661 acgaccgtgg ggtgcgtatg cgtctgtggc ccgtttgagg gagccacagg atggttgtag
     9721 cgtttccaca tactccttcg gcggcagata cgaagggctg accagcatga ctacctcgga
     9781 tgccgacaag gctgtcgcgg acaacgaatc gccgcacggc gctgagacac cggctggagg
     9841 cgagggcccc accaccgaga agcacccgtc gcagcgggcc taccatcatc cggccgccgg
     9901 ctggggtgcc gccaagagcg tcacgcacgt cctcctgcgg gaacacacgc tgatcgacgg
     9961 gccacgcgcc atcttcaaga tgaaccacga gagcgggggg gttcgactgc ccggggtgcg
    10021 cgtggcccga cgacaccaag ggtctcaagc tcgacatctg tgagaacggc atcaagcacg
    10081 tcacctggga gatgacgccc aagcgtgccg gacgtgagct gttcgccgcg cactccgtct
    10141 cggagctggc gacatggacc gacttcgcgc tggagggcac gggcaggctg acggagccgc
    10201 tggtctacaa cgaggagacg gaccactacg agcccatcac ctggaaggcc gcgttcgagc
    10261 tcgtcggaag cgagctgcgg gccctcgacg acccgaacga agccgccttc tacacctcgg
    10321 gacgcctcgg caacgaggcg acgttcctgt accagttgat ggcccgtgag tacggcacca
    10381 acaatctgcc cgactgctcc aacatgtgcc acgaggcgtc cggacgcgcc ctgcaggcgt
    10441 cgctcggcac cggaaagggc accgccgacc tcgccgactg ggagagtgcc gacgccctgt
    10501 tcatcatggg cgtcaacgcg gcctccaacg ctccgcgcat gctgaccgcg ctcgccgagg
    10561 catatcggcg gggtgcgcag atcgtgcacg tcaatccact catcgaggcg gcagccaccc
    10621 ggaccatcgt cccgcacgag ttcgtcgaca tggccctgtt caagtcgacg cgcacgagca
    10681 cgctcaacct gcaggtccgt gccggcggcg acatggcgct cctgcgcggc atggccaagg
    10741 cggtgatcga ggagtcggcc tcggacccga aggcactcga ccgcgagttc atcgaccg
//
