LOCUS       NZ_BMUQ01000006        10405 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces mirabilis strain JCM 4551 sequence06, whole genome
            shotgun sequence.
ACCESSION   NZ_BMUQ01000006
VERSION     NZ_BMUQ01000006.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces mirabilis
  ORGANISM  Streptomyces mirabilis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 531776)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 117X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014650275.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:52:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 10,392
            CDSs (total)                      :: 10,311
            Genes (coding)                    :: 9,898
            CDSs (with protein)               :: 9,898
            Genes (RNA)                       :: 81
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 414
            CDSs (without protein)            :: 413
            Pseudo Genes (ambiguous residues) :: 0 of 414
            Pseudo Genes (frameshifted)       :: 172 of 414
            Pseudo Genes (incomplete)         :: 329 of 414
            Pseudo Genes (internal stop)      :: 40 of 414
            Pseudo Genes (multiple problems)  :: 115 of 414
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 11:21:23
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 431093
            Orig. end                         :: 441498
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMUQ01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5405)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            869..1477
                     /locus_tag="IE289_RS18480"
                     /old_locus_tag="GCM10010317_038030"
     CDS             869..1477
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="IE289_RS18480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_038030"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_054232288.1"
                     /transl_table=11
                     /translation="MDVELPELPFPLRTYGPDGHWSHEDGILTGWAGPRQDRFVPPTDE
                     ALDPASDAPRLLGAPEGDFQLIARVTVGFGAAFDAGVLYVHVGERAWAKLCLEYSPDVP
                     TVCTVVTRGHSDDANSFTVDGSSVWLRISRTGRAFAFHASRDGKRWTFIRLFTLGEEKE
                     TSAALVGFMTQSPMGEGCVVTYDGIEFRPNWPKDLRDGS"
     gene            1542..2048
                     /locus_tag="IE289_RS18485"
                     /old_locus_tag="GCM10010317_038040"
     CDS             1542..2048
                     /EC_number="2.3.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436205.1"
                     /locus_tag="IE289_RS18485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_038040"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_190170342.1"
                     /transl_table=11
                     /translation="MISTPRTTGPLVIRTALPSEAAAITELHVRARSTYYPDGLPFGDV
                     DWVAAWRGSIERPEAHVLCAVRDGHLVGIASFRTADDAAADTVKLFQFHVDPDCWRQGI
                     GRALHTACLEQWRADGKHTASLDVHVDNARAQAFYARQGWVPDPENPPAEGDHHLFLRY
                     AVTGE"
     gene            2126..2845
                     /locus_tag="IE289_RS18490"
                     /old_locus_tag="GCM10010317_038050"
     CDS             2126..2845
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067467.1"
                     /locus_tag="IE289_RS18490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_038050"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_190170343.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALDAFPHRDEVEVVHRSFELDP
                     GRAKGDIQPVLKMLIKKYGMSEAQAQAGEENLGAQAAAEGLAYRTRDRDHGNTFDMHRL
                     LHFAKERGRQDELIGLLYKANFAEERTVFNDDERLVELAVTAGLDADASREVLADPKAY
                     ADEVRADEREAAELGANGVPFFVLDRKYGVSGAQPAEVFAQALNQAWGERPPLKLIQDG
                     AADAEACGPDGCAVPQP"
     gene            2961..4010
                     /locus_tag="IE289_RS18495"
                     /old_locus_tag="GCM10010317_038060"
     CDS             2961..4010
                     /NRPS_PKS="Domain: Aminotran_5 (16-343). E-value: 2.7e-24.
                     Score: 77.7. Matches aSDomain:
                     nrpspksdomains_IE289_RS18495_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477739.1"
                     /locus_tag="IE289_RS18495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_038060"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_229889001.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.5e-19, bitscore:
                     64.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFESLVRAEFAPKNTYLNTASCGLLPARTVEAMRAAVESVADG
                     RPSDMFDDVEAARAAFARLAGVPAGRVAAGASVAVYSGLIAASLPPGAEVLTAEDDFSS
                     VVNPFHVRGDLKLRAVPLERIAESVRPGTALVAVSAAQSADGRIADLAGIARAAREHGA
                     RTYVDVSQAAGWLPMEADAYDFVSSVAFKWLVCPRGVAFLVVPEDLGGLTPVFAGWVAG
                     EAPWDSCYGPVAELAHSARRFDESPSLFSYAGARHSLALVEELGVANVRAHDLALAERF
                     RAGLQGLGHAPVSAPASPIVSVPGLGRRQQELSEAGVEVSDRAGNLRAAFHLYNTPADV
                     DRLLDVLSG"
     aSDomain        3009..3989
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE289_RS18495_Aminotran_5.1"
                     /evalue="2.70E-24"
                     /label="IE289_RS18495_Aminotran_5.1"
                     /locus_tag="IE289_RS18495"
                     /protein_end="343"
                     /protein_start="16"
                     /score="77.7"
                     /tool="antismash"
                     /translation="TYLNTASCGLLPARTVEAMRAAVESVADGRPSDMFDDVEAARAAF
                     ARLAGVPAGRVAAGASVAVYSGLIAASLPPGAEVLTAEDDFSSVVNPFHVRGDLKLRAV
                     PLERIAESVRPGTALVAVSAAQSADGRIADLAGIARAAREHGARTYVDVSQAAGWLPME
                     ADAYDFVSSVAFKWLVCPRGVAFLVVPEDLGGLTPVFAGWVAGEAPWDSCYGPVAELAH
                     SARRFDESPSLFSYAGARHSLALVEELGVANVRAHDLALAERFRAGLQGLGHAPVSAPA
                     SPIVSVPGLGRRQQELSEAGVEVSDRAGNLRAAFHLYNTPADVDRL"
     gene            complement(4074..4994)
                     /gene="thpD"
                     /locus_tag="IE289_RS18500"
                     /old_locus_tag="GCM10010317_038070"
     CDS             complement(4074..4994)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006143403.1"
                     /locus_tag="IE289_RS18500"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_038070"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_190170345.1"
                     /sec_met_domain="PhyH (E-value: 1e-39, bitscore: 131.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTTTSTTSTTNVTDLYPSRGAAEVSVPRQDPVVWGAPGTPGP
                     FSVPDLQAYERDGFLAIEELIGEDDVALYRAELERLVTDPAIRADERSIVEPATQEIRS
                     VFEVHRISEVFAALVRDERVVGRARQILGSDVYVHQSRINVKPGFGAGGFYWHSDFETW
                     HAEDGLANMRTVSVSIALTENHDTNGGLMIMPGSHKTFLGCAGATPRDNYKKSLQMQDA
                     GTPSDEALTRFADRHGIKLFTGRAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVEN
                     TAVEPFAAPVRRPEFIGSRDFTPVK"
     gene            complement(5001..5405)
                     /locus_tag="IE289_RS18505"
                     /old_locus_tag="GCM10010317_038080"
     CDS             complement(5001..5405)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016643612.1"
                     /locus_tag="IE289_RS18505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_038080"
                     /product="ectoine synthase"
                     /protein_id="WP_099923372.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.9e-54, bitscore:
                     177.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVKAATGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCVAGEAELTNDETGETHWISPGTMYLLDGHERHTMRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPVLTEPEEV"
     gene            complement(5468..6739)
                     /gene="ectB"
                     /locus_tag="IE289_RS18510"
                     /old_locus_tag="GCM10010317_038090"
     CDS             complement(5468..6739)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 5.4e-72.
                     Score: 234.6. Matches aSDomain:
                     nrpspksdomains_IE289_RS18510_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="IE289_RS18510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_038090"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_190170346.1"
                     /sec_met_domain="Aminotran_3 (E-value: 2.1e-78, bitscore:
                     258.6, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTIIQPDLSVFETLESEVRSYCRSWPTVFDRARGSRMFDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTTAKRAFLEAFQNLVLRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKAREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGQVPDFLWFERLLEDQGSGLNQPAAVIVETVQGEGGINVARP
                     EWLRALADLCRRRDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPMS
                     LCLFKPELDVWEPGEHNGTFRGNNPAFVTAAAALETYWSDGSAMEKQTRARGEQIEQAL
                     ISVTEENLADVKEYRGRGLVWGLEFHEKERAGRVAQRAFELGLLIETSGPESEVVKLLP
                     ALTITPDELDEGLRTLARAVRETV"
     aSDomain        complement(5642..6655)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE289_RS18510_Aminotran_3.1"
                     /evalue="5.40E-72"
                     /label="IE289_RS18510_Aminotran_3.1"
                     /locus_tag="IE289_RS18510"
                     /protein_end="366"
                     /protein_start="28"
                     /score="234.6"
                     /tool="antismash"
                     /translation="FDRARGSRMFDEDGHEYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTTAKRAFLEAFQNLVLRPRDLPYKVMFPGPTGTNAVESALKLARKVKA
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGQVPDFLWFER
                     LLEDQGSGLNQPAAVIVETVQGEGGINVARPEWLRALADLCRRRDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPMSLCLFKPELDVWEPGEHNGTFRGNNPAFV
                     TAAAALETYWSDGSAMEKQTRARGEQIEQALISVTEENLADVKEYRGRGLVWGLEFH"
     gene            complement(6838..7377)
                     /gene="ectA"
                     /locus_tag="IE289_RS18515"
                     /old_locus_tag="GCM10010317_038100"
     CDS             complement(6838..7377)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987798.1"
                     /locus_tag="IE289_RS18515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_038100"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_190170347.1"
                     /transl_table=11
                     /translation="MTAAQADLQAEFLEMPEGLRFDRPKVADGAALWHIAKDSGTLDLN
                     SSYSYLLWCRDFAGTSAVARDADGVPVGFVTGYVRPERPGTLLVWQVAVDAAHRGRGLA
                     AALLDGLTARVTEQRTITAVETTITPGNTASERLFTSYARRHGARVEHEVLFEAGQFPD
                     GPHDPEVLYRIGPLSS"
     misc_feature    complement(7402..7414)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(7533..7548)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     gene            complement(7912..9012)
                     /locus_tag="IE289_RS18520"
                     /old_locus_tag="GCM10010317_038110"
     CDS             complement(7912..9012)
                     /NRPS_PKS="Domain: Aminotran_5 (73-283). E-value: 1.3e-26.
                     Score: 85.3. Matches aSDomain:
                     nrpspksdomains_IE289_RS18520_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005477727.1"
                     /locus_tag="IE289_RS18520"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_038110"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_229889008.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.6e-21, bitscore:
                     70.8, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAAHFAAIEDRVARLLSTEQDVVIMQGEALLPLE
                     GAIRATAGPGTTALNVITGPYGQTFGDWLRDCGATVIDLTVPFHTAVTAAQVRDAFAEH
                     PAIDFVSLVHAEAATGNTNPVAEIGEVVRAHGALFYLDAVASIGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSDRAWARMAANPGAPRRSYLSLLDWKERWVDGGRKALLHAP
                     AQLEMLALEACVERIEGEGLDAVMRRHALAAAATRAGAAALGGGLEPYVYETEDAAPVA
                     TTLRAPSGVDAGVLVAKALAADPVLPLVAGGGALAKEMIRVNHYGPDATPGVVHSCLAA
                     LGAAMAESGLTIDLEAARRATTKAWA"
     aSDomain        complement(8164..8793)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE289_RS18520_Aminotran_5.1"
                     /evalue="1.30E-26"
                     /label="IE289_RS18520_Aminotran_5.1"
                     /locus_tag="IE289_RS18520"
                     /protein_end="283"
                     /protein_start="73"
                     /score="85.3"
                     /tool="antismash"
                     /translation="LRDCGATVIDLTVPFHTAVTAAQVRDAFAEHPAIDFVSLVHAEAA
                     TGNTNPVAEIGEVVRAHGALFYLDAVASIGAEPVLPDAWGVDLCVIGAQKAMGGPAGVS
                     AVSVSDRAWARMAANPGAPRRSYLSLLDWKERWVDGGRKALLHAPAQLEMLALEACVER
                     IEGEGLDAVMRRHALAAAATRAGAAALGGGLEPYVYETEDAAPVATT"
     misc_feature    complement(9124..9138)
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: medium, score: 25.84"
                     /tool="antismash"
     gene            9250..10098
                     /locus_tag="IE289_RS18525"
                     /old_locus_tag="GCM10010317_038120"
     CDS             9250..10098
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121392.1"
                     /locus_tag="IE289_RS18525"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010317_038120"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_190170349.1"
                     /transl_table=11
                     /translation="MNSVLGRRTRILAATTATAGLLLVAGCSSGGGGGQSSAKGVPLVK
                     AGQVTTCTHLPYPPFQSEINGKVQGFDVALIDLVAGNLGVKQAIVDTPFENFKTGAFLN
                     SGQCDLAAAGMTITPERKKNVDFSDPYFDATQAVLVDKKSGVNSLADVKAKGKKLGAQA
                     QTTGEEYAAKQGFDSVSFESSDAVLNGLRTGQVQAVVIDYPVVQGWLKDKANADAFKVV
                     DNLNTGEQYGFTVKKGNTKLLAEINKAIKQAKADGTYKKLYEKWIGPYPASAATASPSA
                     S"
ORIGIN
        1 ccgcggcggc cgagcgcgcc gaggctatcg gcgccgggct cgcgggctcg atgacgctgg
       61 gcgtcggcca gttctgcgtg aagccgggcc tggtcctggc gcccgcgggc gcggcgggcg
      121 accgtctggt gaagtccctg accgacgccg tcagcgacac cgacgcgggc gtcctcctgg
      181 accatcgcat gcgcgacaac ttcatcgcgg gtgtcgccga gcgcgccggg ctccccggcg
      241 tcgagagccc ggtgaccccg ggctcgggcg gcgagcacac ggtgagcccc ggcttcctga
      301 ccgtgcccgc gcagaagctc gtcaccgagg gcgagcacga cctgctcctg gaggagtgct
      361 tcggcccgct cacggtcgtc gcccgctacg aggacgagtc cgaggccagc gcggtcctct
      421 cgcgccttcc cggcaacctc accgcgaccg tgcagctgtc ggcggaggag gcggcgggcg
      481 aaggccgcgg cgcggagatc ctcgccgagc tgacgccgct cgccgggcgc gtcctggtca
      541 acggctggcc caccggtgtc gccgtcgccc ccgcccagca ccacggcggt ccctacccgg
      601 cgacgacgtc gacctcgacg tcggtcggcg gtacggcgat cgagcgctgg ctgcggccgg
      661 tggcgtacca gaacgcgccg gaggcgctgc tgccgccgga gctgcgggac gacaacccgc
      721 tggggttgcc gcgacggtac gacggccgtc tggagcggta gcgccgtcgg ggttgtggtt
      781 gttcggcggc tgcagggccg ttgtggctgg tcgcgcagtt cctcgcgccc ctttcggggc
      841 gcggtacctg gcctgagagg attggtgaat ggacgtcgaa cttcccgaac ttcccttccc
      901 gctccgtacc tacgggcctg acgggcactg gtcgcacgag gacgggatac tcaccggctg
      961 ggccggcccc cgccaggacc gtttcgtacc gcccaccgac gaggcactgg accccgcctc
     1021 cgacgcgccc cgtctcctcg gggcgcccga gggggacttc cagctgatcg cccgcgtcac
     1081 ggtcggcttc ggcgcggcct tcgacgcggg ggtcctctac gtccatgtgg gggagcgggc
     1141 ctgggcgaag ctctgcctgg agtactcccc ggacgtgccc accgtctgca cggtggtcac
     1201 ccgggggcac tccgacgacg ccaactcctt caccgtggac ggcagttccg tctggctgcg
     1261 gatcagccgc acgggtcggg ccttcgcctt ccacgcctcc cgcgacggca agcgctggac
     1321 gttcatccgg ctcttcaccc tgggtgagga gaaggagacg tccgccgcgc tcgtcggctt
     1381 catgacgcag tcgccgatgg gggaggggtg cgtggtgacg tacgacggca tcgagttccg
     1441 cccgaactgg cccaaggacc tgagagacgg cagctgaccg tgggtggggg gtcggccgcg
     1501 gggtgggaac cgtggccccc ctccaccacg tcctccgctg catgatcagc acaccccgca
     1561 ccacgggccc cctcgtcatc agaaccgcgc tgcccagcga ggccgccgcc atcaccgagc
     1621 tgcacgtccg cgcccgctcc acgtactacc cagacggcct gcccttcggc gacgtggact
     1681 gggtcgccgc gtggcggggc agcatcgagc gccccgaggc ccatgtcctg tgcgcggtgc
     1741 gtgacggaca cctggtgggc atcgcctcct tccggacggc cgacgacgcg gccgccgaca
     1801 ccgtgaagct cttccagttc cacgtcgatc ccgactgctg gcgccagggc atcggccggg
     1861 cactgcacac ggcctgcctc gaacagtggc gggccgacgg caagcacacg gccagcctcg
     1921 acgtgcacgt cgacaacgcc cgcgcccagg ccttctacgc ccgccagggc tgggtcccgg
     1981 acccggagaa cccgcccgcc gaaggcgatc accacctctt cctgcggtac gccgtcaccg
     2041 gggaatgaaa tcggccgttt gaacgttcac atactcctgc ccccggaggg gcccgtattc
     2101 gtacgacctg gagagtgccg aagacatgcg cgtcgagatc tggtcggata tcgcctgtcc
     2161 ctggtgctac gtgggcaagg cccgcttcga gaaggcgctg gacgccttcc cgcaccgtga
     2221 cgaggtcgag gtcgtgcacc gctccttcga gctggacccc ggtcgcgcca agggcgacat
     2281 ccagcccgtg ctcaagatgc tgatcaagaa gtacggcatg agcgaggcac aggcgcaggc
     2341 cggcgaggag aacctgggcg cgcaggccgc cgccgagggc ctggcgtacc gcacccggga
     2401 ccgcgaccac ggcaacacct tcgacatgca ccgcctcctg cacttcgcca aggagcgggg
     2461 ccgccaggac gagctgatcg gtctgctgta caaggcgaac ttcgccgagg agcggaccgt
     2521 cttcaacgac gacgagcggc tcgtcgagct ggccgtcacc gccgggctcg acgccgacgc
     2581 gtcccgcgag gtgctggccg acccgaaggc gtacgccgac gaggtgcgcg ccgacgagcg
     2641 cgaggccgcc gagctcggcg cgaacggcgt gccgttcttc gtcctggacc gcaagtacgg
     2701 cgtctccggc gcccagcccg cagaggtgtt cgcacaggcc ctgaaccagg cgtggggcga
     2761 gcgcccgccg ctgaagctga tccaggacgg tgccgccgac gcggaggcct gcggcccgga
     2821 cggatgcgcc gtaccgcagc cgtaagccgt ccggcacggc ggtgaaagcg caggtcgggg
     2881 cggacgtata agcagtcctt accggaacca ctcaaaactc cgtaatggac gagaggtgct
     2941 cgggaccgca cagtggtccc atggagacct tcgagagcct cgtacgagcc gagttcgccc
     3001 cgaagaacac ctatctgaac accgcgagct gcggactcct gccggcgcgc acggtcgagg
     3061 ccatgcgcgc cgccgtcgag tccgtcgccg acgggcggcc gtccgacatg ttcgacgacg
     3121 tggaggccgc ccgcgccgcc ttcgcccggc tggccggggt ccccgcgggc cgggtggcgg
     3181 ccggggcctc ggtcgccgtc tacagcggac tgatcgccgc ctcgctgccg ccgggcgccg
     3241 aagtcctcac cgcggaggac gacttcagct ccgtcgtgaa ccccttccac gtccgtggcg
     3301 acctcaagct gcgcgccgta ccgctggagc ggatcgccga gtccgtacgt ccgggcaccg
     3361 cgctcgtcgc ggtcagcgcc gcgcagtccg cggacgggcg gatcgccgat ctggcgggca
     3421 tcgcccgggc cgcgcgcgag cacggggcgc gtacgtacgt cgacgtctcc caggccgccg
     3481 gctggctgcc gatggaggcc gacgcgtacg acttcgtctc ctcggtcgcc ttcaagtggc
     3541 tcgtctgccc tcggggcgtg gccttcctgg tcgttccgga ggacctcggc gggctcactc
     3601 cggtgttcgc cggctgggtc gcgggtgagg cgccctggga cagctgctac ggcccggtgg
     3661 cggaactcgc ccactcggcg cggcggttcg acgagagccc cagcctcttc tcgtacgcgg
     3721 gcgcccggca ctcgctggcg ctcgtcgagg agctgggcgt ggcgaacgta cgcgcccatg
     3781 acctcgccct cgccgagcgt ttccgcgccg ggctccaggg gctcggccac gcgccggtct
     3841 ccgcccccgc ctcgccgatc gtgtccgtgc ccggactcgg ccggcggcag caggagttga
     3901 gcgaggccgg ggtcgaggtc tcagaccggg cgggcaatct gcgcgcggcc ttccacctgt
     3961 acaacacccc cgccgacgtc gaccgactgc tggacgtcct gtccggctga ccccggcggg
     4021 ggcttcttca cgctcgtggc tacgtcacgc gcgcggtcac ttcacgctcg cggtcacttc
     4081 acgggcgtga aatcacggga gccgatgaac tcgggcctgc ggaccggcgc cgcgaacggc
     4141 tccaccgccg tgttctccac gctgttgaac acgatgaaga cgttgctgcg cgggaacggg
     4201 gtgatgttgt ccccggagcc gtgcatgcag ttgcagtcga accaggtcgc cgaaccggcc
     4261 cggcccgtga agagcttgat gccgtgccgg tcggcgaagc gggtcagcgc ctcgtcggac
     4321 ggggtgcccg cgtcctgcat ctggagcgac ttcttgtagt tgtcccgcgg ggtggctccc
     4381 gcacagccga ggaacgtctt gtgcgacccc ggcatgatca tgaggccgcc gttggtgtcg
     4441 tggttctcgg tcagcgcgat cgacacggac acggtccgca tgttcgccag cccgtcctcg
     4501 gcgtgccagg tctcgaagtc cgagtgccag tagaacccgc cggccccgaa gcccggcttg
     4561 acgttgatcc gcgactggtg gacgtagacg tccgagccga ggatctgccg ggcccgcccg
     4621 acgacccgct cgtcgcgtac gagcgcggcg aagacctcgc tgatgcggtg cacctcgaag
     4681 acggaccgga tctcctgcgt ggcgggctcg acgatcgagc gctcgtcggc gcggatcgcc
     4741 ggatcggtga ccagccgctc caactcggcc cggtagaggg cgacgtcgtc ctcgccgatc
     4801 agctcctcga tcgcgaggaa gccgtcacgc tcgtacgcct ggaggtcggg cacggagaac
     4861 ggccccggcg tgccgggcgc gccccagacg accgggtcct ggcgggggac ggacacctcg
     4921 gcggcgccgc ggctggggta gagatcggtg acgttggtcg tgctggtcgt gctggtcgtg
     4981 gtggtcgtgg tcatggggct tcagacctcc tcgggttcgg tgagcaccgg gtagacgccg
     5041 ttctcgtcgt ggtcctcccg tccggtgacg ggcggattga agacgcagac gcaccggaag
     5101 tcggtcttgg ggcgcatggt gtggcgctcg tggccgtcga gcaggtacat cgtgcccggc
     5161 gagatccagt gcgtctcacc ggtctcgtcg ttggtcagct cggcctcgcc cgccacgcag
     5221 agcacggctt cgatgtggtt cgcgtaccac atcgacgtct ccgtacccgc gtacaggacc
     5281 gtctcgtgga gggagaagcc caccttctcc ttggcgagga cgatgcgctt gctctcccag
     5341 gtacccgtcg cggccttcac atgccggtcg gtgccttcga tgtccttgaa cgaacggact
     5401 atcacggtgg tgccgtgcct ttctgtcaga tctgtcagat gtctgccgga cgtctgtcgg
     5461 tcgtctgtca gacggtttcg cgtacggcgc gggcgagggt gcgcaggccc tcgtcgagct
     5521 cgtcgggggt gatggtgagc gcggggagga gtttcacgac ctcgctctcc ggccctgacg
     5581 tctcgatgag cagcccgagt tcgaaggcgc gctgcgcgac gcgccccgca cgctccttct
     5641 cgtggaactc gaggccccag acgagaccac gcccccggta ctccttcacg tcggcgaggt
     5701 tctcctcggt gacggagatc agggcctgct cgatctgctc gccgcgggca cgggtctgct
     5761 tctccatggc ggacccgtcg gaccagtacg tctccagggc cgcggcggcg gtgacgaacg
     5821 cggggttgtt gccgcggaac gtgccgttgt gctcgcccgg ctcccagacg tccagttcgg
     5881 gcttgaacag gcacagcgac atgggcagcc cgtacccgct gatggacttg gacacggtca
     5941 cgatgtcggg cacgatcccc gcctcctcga aggagaagaa ggctcccgtc cgcccgcagc
     6001 ccatctggat gtcgtcgacg atgagcagca tgtcgcgccg ccggcacagg tcggcgaggg
     6061 cgcgcagcca ctcgggccgg gcgacgttga tgccgccctc gccctgcacg gtctcgacga
     6121 tgaccgcggc gggctggttg agccccgacc cctggtcctc gagcagccgc tcgaaccaga
     6181 ggaagtccgg cacctggccg tcgaagtagt tgtcgaacgg catcggggtc ccgtgcacga
     6241 gcggaatccc ggcaccggcc cgcttgaagg cgtttccggt cacggccagc gagcccagcg
     6301 acatcccgtg gaaggcgttg gtgaaggaca cgatcgcctc gcgcgccttc accttccgcg
     6361 ccagcttcag cgcggactcg acggcgttgg tgcccgtcgg ccccgggaac atgaccttgt
     6421 acggcaggtc gcgcggccgt aggacgaggt tctggaaggc ctccaggaag gcgcgcttgg
     6481 cggtcgtcga catgtcgaga ccgtgggtga cgccgtcgcg ctccaggtag tcgatgaggg
     6541 cccgtttgag tacggggttg ttgtgcccgt agttgaggga ccccgcaccg gcgaagaagt
     6601 cgaggtactc gtgcccgtcc tcgtcgaaca tccggctgcc ccgggcccgg tcgaagacgg
     6661 tgggccagct gcggcagtag ctgcgcacct ccgactccag ggtctcgaag acgctcaggt
     6721 cgggctggat gatggtcacg gcgaatcgct cctcggtgcg tggggggagg gggagaagtc
     6781 agggggaagc caggggaagc cagggggtgt ccgggtcggc cgggggccgg cccggggtca
     6841 gctggagagc ggaccgatcc ggtagagcac ttcggggtcg tgcggtccgt ccgggaactg
     6901 cccggcctcg aacagcactt cgtgctcgac gcgcgcgccg tggcgtcgcg cgtacgaggt
     6961 gaacaggcgc tcggaggcgg tgttgccggg cgtgatcgtg gtctcgacag cggtgatcgt
     7021 ccgctgctcc gtgacccgcg cggtcagccc gtcgagcagg gccgcggcca gtccgcgtcc
     7081 gcggtgggcg gcgtcgacgg ccacctgcca cacgagcagg gtgcccggac gctcgggccg
     7141 gacgtacccg gtgacgaagc cgacgggcac gccgtccgcg tcgcgcgcca ccgccgacgt
     7201 gccggcgaag tcacggcacc acagcagata gctgtacgag gagttcaggt cgagggttcc
     7261 ggagtccttg gcgatatgcc agagcgcggc tccatccgcc accttcggac ggtcgaaccg
     7321 aaggccctcc ggcatttcca ggaattccgc ttgcaggtct gcttgtgcgg cggtcatgcg
     7381 gattgaattt accgagggaa atctcaaatt gcatcgtcgt ccggggttac gcacgacgcg
     7441 ccgtcgtgtt atcacgcgcc cgtgagagcc ctgtgctgcg ggtggtgtaa agggacggtt
     7501 tgcccggaaa ataggggagg aagcggacgc attgtggagt gcgtcacaga cacgtaatgg
     7561 ccacgagatg tgcccgaatt acgtcgtcgg cgttcgcgaa atctttgcgt ttagggtcca
     7621 ggaaagcggg cagaagaata cgggaagcta atcttcgagt taatgtaaga ataagcatgg
     7681 tgagtgcctc gtcaattgat gcacgattaa gccccggtaa gcccggcgat tccctcggct
     7741 cgtcgcgcag ttccccgcgc ccctggggca cggggctgcg ccccggcgcc ccatcggcct
     7801 gacggcctcg tcctcacgcg ccggacgagc tggggggctt ttaggggcgc ggggaactgc
     7861 gcgaccagcc cccacccacc cgcagccgac acactcaccc gtggagtacg gctaggccca
     7921 ggccttcgtc gtcgcgcggc gtgccgcttc caggtcgatc gtgaggccgg actcggccat
     7981 cgccgccccc aacgccgcga ggcaggagtg cacgaccccc ggcgtcgcat cggggccgta
     8041 gtggttcacc cggatcatct ccttcgccag cgcaccccca cccgcgacca acggcaacac
     8101 cggatccgcg gccaacgcct tcgcgaccag cacccccgca tcgaccccgg acggtgcccg
     8161 caacgtcgtg gcgaccggcg ccgcgtcctc cgtctcgtac acatacggct ccagcccgcc
     8221 accgagagcg gccgcccccg cccgcgtggc cgccgccgcc agggcatggc ggcgcatcac
     8281 cgcgtcgagt ccctccccct cgatccgctc gacacacgcc tccagtgcca gcatctccag
     8341 ctgcgccggc gcgtgcagca gcgccttgcg cccgccgtcg acccaccgct ccttccagtc
     8401 cagcagggag aggtaggacc gacgaggcgc cccgggattg gcggccatcc gcgcccacgc
     8461 ccgatcgctg acagacacgg ccgacacccc ggccggcccg cccatcgcct tctgcgcccc
     8521 gatcacgcac aggtcgactc cccacgcgtc cggcagcacc ggctccgcgc cgatcgaggc
     8581 gacggcgtcg aggtagaaca gcgcgccgtg cgcacgcacc acctcgccga tctccgcgac
     8641 ggggttcgtg ttgcccgtcg ccgcctccgc gtgcaccagc gagacgaagt cgatcgccgg
     8701 atgctcggcg aaggcgtcac gcacctgtgc ggccgtcacc gccgtgtgga acggcaccgt
     8761 gaggtcgatc accgtcgcgc cgcagtcccg cagccagtcc ccgaaggtct gcccgtacgg
     8821 cccggtgatc acgttgagcg cggtggtgcc gggcccggcc gtggcgcgaa tggcaccctc
     8881 caggggcagc agcgcctcgc cctgcatgat cacgacgtcc tgctcggtgg agagcagccg
     8941 cgccacgcgg tcctcgatcg ccgcgaagtg tgcggcgctc agcggggcga ggtccaggaa
     9001 gggatgggtc acggcggtgc tctcttcgct cacgggttcc aattgcgcag ccccgagcgt
     9061 atccggcgtc cttgtcacgc ccgcggcgat cctcggtcgt acgagcggac acccccaggt
     9121 cgtacggccg gacggccccg cgaggcatga cttctgaggc cgaacggcgc aacagccatc
     9181 ggattggttt gagcgagtca aacctttcct tataatcgga atcctccatt ccccccacag
     9241 gaggtctccg tgaactcggt cctcggacgc cggacgcgca ttctggccgc caccaccgcc
     9301 acggccgggc tgctgctcgt ggccggctgt tcctcgggcg gcggaggggg ccagagcagc
     9361 gccaagggcg ttcccctggt caaggcgggc caggtcacga cctgcaccca cctcccgtat
     9421 ccgccgttcc agtcggagat caacggcaag gtgcagggct tcgacgtcgc gctgatcgat
     9481 ctggtcgcgg ggaacctcgg cgtgaagcag gccatcgtcg acacgccctt cgagaacttc
     9541 aagacgggcg cgttcctcaa ctccggccag tgcgacctcg ccgcggccgg catgacgatc
     9601 accccggagc gcaagaagaa cgtcgacttc tccgatccgt acttcgacgc cacccaggcc
     9661 gtcctggtcg acaagaagag cggcgtcaac tccctcgccg acgtgaaggc caagggcaag
     9721 aagctcgggg cccaggcgca gaccacgggc gaggagtacg cggcgaagca gggcttcgac
     9781 tcggtctcct tcgagtcctc cgacgcggtc ctcaacggcc tgcgcaccgg ccaggtccag
     9841 gccgtcgtca tcgactaccc ggtcgtccag ggctggctga aggacaaggc gaacgccgac
     9901 gccttcaagg tggtcgacaa cctcaacacc ggtgagcagt acggcttcac ggtcaagaag
     9961 ggcaacacca agctcctcgc cgagatcaac aaggcgatca agcaggccaa ggccgacggc
    10021 acgtacaaga agctctacga gaagtggatc ggcccgtacc ccgcgtccgc ggccaccgcc
    10081 tcgccgtccg cctcatgagc gatgccgaca cacgactcca gccccgaaag tccggcctga
    10141 cccggcgcca gaagcgcgcc ctctcgcgcg gcgcgcagta cgtcgtcttc gtcgccgccg
    10201 tgatcgcctt cgcggtcgcg gcggactggg gacgtctgaa gaaccagttc gcgcagtggg
    10261 acctggtcaa gcagatgttc ccggacgtca tcacactggc gctgaagaac accgtgctct
    10321 acacgatgtc cggcttcgtc gtcggtctcg tgctcggcct ggtcatcgcc ctgatgcggc
    10381 tgtccccggt gggcccctac cgctg
//
