LOCUS       NZ_BMTZ01000046        13473 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces variabilis strain JCM 4422 sequence46, whole genome
            shotgun sequence.
ACCESSION   NZ_BMTZ01000046
VERSION     NZ_BMTZ01000046.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces variabilis
  ORGANISM  Streptomyces variabilis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces griseoincarnatus
            group.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 13473)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 156X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649935.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:37:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,743
            CDSs (total)                      :: 6,668
            Genes (coding)                    :: 6,496
            CDSs (with protein)               :: 6,496
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 61 of 172
            Pseudo Genes (incomplete)         :: 147 of 172
            Pseudo Genes (internal stop)      :: 11 of 172
            Pseudo Genes (multiple problems)  :: 42 of 172
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:23:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 13473
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMTZ01000046.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..91
                     /locus_tag="IE148_RS32870"
                     /pseudo=""
     source          1..13473
                     /culture_collection="JCM:4422"
                     /db_xref="taxon:67372"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces variabilis"
                     /strain="JCM 4422"
                     /submitter_seqid="sequence46"
                     /type_material="type strain of Streptomyces variabilis"
     region          1..13473
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..13473
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..13473
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[4935:11662](+)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      4936..11662
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     CDS             <3..91
                     /codon_start=2
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983099.1"
                     /locus_tag="IE148_RS32870"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="DVAPIGDVRWVEIDNHDDLARGREIACQY"
     gene            79..1140
                     /locus_tag="IE148_RS32875"
                     /old_locus_tag="GCM10010287_66340"
     CDS             79..1140
                     /GO_function="GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DHQ_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1181: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494151.1"
                     /locus_tag="IE148_RS32875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_66340"
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="WP_048460824.1"
                     /sec_met_domain="Fe-ADH (E-value: 2.4e-22, bitscore: 69.2,
                     seeds: 218, tool: rule-based-clusters)"
                     /sec_met_domain="DHQ_synthase (E-value: 1.6e-09, bitscore:
                     27.3, seeds: 76, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVLTRLIPSPVVVDIRPGALDDLACVLADERISQSGRLAIAVSG
                     GSGARLRDRVAPSLPGADWFEVGGGTIDDAVRLAGAIQGGRYDAVVGLGGGKIIDCAKF
                     ASARVGLPLVAVATNLAHDGLCSPVATLDNDAGRGSYGVPNPIAVVIDLNVIREAPVRF
                     VRSGIGDVVSNISAVADWELSHRVNGEKVDGLAAAMARQAGEAVLRHPGGVDDDSFLQV
                     LAEALVLTGISMSVSGDSRPSSGACHEINHAFDLLYPQRAAAHGEQCGLGAAFAMYLRG
                     AHEESAAMAEVLRRHGLPVLPEEIGFSVDEFVRVVEYAPETRPGRFTILEHLALTTEQI
                     KDVYADYVKAIGS"
     gene            1154..1897
                     /locus_tag="IE148_RS32880"
                     /old_locus_tag="GCM10010287_66350"
     CDS             1154..1897
                     /EC_number="2.7.8.-"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /GO_process="GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676198.1"
                     /locus_tag="IE148_RS32880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_66350"
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="WP_078895344.1"
                     /transl_table=11
                     /translation="MHPAGVKDRRSGEHWMGRLYMREVSLRVDRYLVNTRVTPNQLTYL
                     MTLCGVLAAPALLVPGITGAVLGVVAVQLYLLLDCVDGEIARWRKQYSLAGVYMDRVGA
                     YLTDAAVLVGLGLRAADLWGTGRIDWLWAFLGTLAALGAILIKAETDLVGVARHQNGMP
                     PVKESASEPRSSGMALARKAAAALKFHRLILGIEASLLILLLAILDSARGDLFFTRLGT
                     AVLAGIALLQTLLHLVSVLVSSRLK"
     gene            1906..2778
                     /locus_tag="IE148_RS32885"
                     /old_locus_tag="GCM10010287_66360"
     CDS             1906..2778
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339265.1"
                     /locus_tag="IE148_RS32885"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_66360"
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_373302333.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 2.8e-15,
                     bitscore: 46.7, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKVGAVIITMGNRPDELRALLDSVAKQDGDPVEVVVVGNGSPVPD
                     VPEGVKTVELPENLGIPGGRNVGIEAFGPSGRDVDILLFLDDDGLLAHTDTAELCRKAF
                     AEDPSLGIISFRIADPDTGVTQRRHVPRLRASDPMRSSRVTTFLGGANAVRTQVLAEVG
                     GLPDEFFYAHEETDLAWRALDAGWMIDYRSDMVLYHPTTAPSRHAVYHRMVARNRVWLA
                     RRNLPVPLVPVYLGVWLLLTLLRRPSGSALKAWFGGFKEGWTSPCGPRRPMKWRTVWRL
                     TRLGRPPVI"
     gene            2875..>3110
                     /locus_tag="IE148_RS33995"
                     /pseudo=""
     CDS             2875..>3110
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994380.1"
                     /locus_tag="IE148_RS33995"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="ABC transporter permease"
                     /pseudo=""
                     /transl_table=11
                     /translation="MSETTHDGTVAVGAPVPPDEGLPAAELAAKYGLSVSGARPSLGEY
                     VRQLWGRRHFILAFSQAKLTAQYSKAKLGQLWQ"
     assembly_gap    3111..3138
                     /estimated_length=28
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3139..3832
                     /locus_tag="IE148_RS32890"
                     /old_locus_tag="GCM10010287_66370"
     CDS             <3141..3832
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994380.1"
                     /locus_tag="IE148_RS32890"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_66370"
                     /product="ABC transporter permease"
                     /protein_id="WP_246539278.1"
                     /transl_table=11
                     /translation="PLLNAAVYFFIFGLLLGARRGIPSDVYVPFLVTGVFVFTFTQSSV
                     LAGVRAISGNLGLVRALHFPRASLPISFALQQLQQLLFSMIVLVVIMVAFRSYPDLSWL
                     LVVPALGLQFVFNTGLALIFARMGSKTPDLAQLMPFVLRTWMYASGVMFSIPAMLEDKN
                     VPSWVADVLQWNPAAVYMDLVRYALIDKYDSTYLPPHIWAFGLGWAVLAGVVGFVYFWK
                     AEERYGRG"
     gene            3825..4604
                     /locus_tag="IE148_RS32895"
                     /old_locus_tag="GCM10010287_66380"
     CDS             3825..4604
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018534466.1"
                     /locus_tag="IE148_RS32895"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_66380"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_086703599.1"
                     /transl_table=11
                     /translation="MADDKTGARVPTVIADDLHIVYRVNGTRSGKGSATAALSRILKRG
                     EERGVRKVHAVRGVSFVAYRGEAIGLIGSNGSGKSTLLRAIAGLLPAERGKVYTDGQPS
                     LLGVNAALMNDLTGERNIILGGLAMGMSREEIKARYQDIVDFSGINEKGDFITLPMRTY
                     SSGMAARLRFSIAAAKDHDVLMIDEALATGDRKFQKRSEARIRELRKEAGTVFLVSHNN
                     KSIRDTCDRVLWLERGELRMDGPTDEVLREYEKFTGK"
     gene            4936..5877
                     /gene="hpnC"
                     /locus_tag="IE148_RS32900"
                     /old_locus_tag="GCM10010287_66390"
     CDS             4936..5877
                     /EC_number="2.5.1.21"
                     /GO_function="GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527751.1"
                     /locus_tag="IE148_RS32900"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_66390"
                     /product="squalene synthase HpnC"
                     /protein_id="WP_373302332.1"
                     /sec_met_domain="phytoene_synt (E-value: 2.5e-38, bitscore:
                     121.9, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PT_phytoene_like (E-value: 6.6e-40,
                     bitscore: 127.2, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRRPLPGQSAMTAAGRTRPGDLERATLDKAAAENFPVAPFFLPRA
                     WRDDLMAVYGYARLVDDIGDGDLAPGGADARALGVPEDKAGDRLTLLDALEADLHRVFD
                     PKGPAPRHPLLLRLEPTVRRRALTPEPFLGLIAANRQDQLVTRYETYDDLLAYCELSAN
                     PVGRLVLAVTGTATPERIRRSDAICTALQIVEHLQDVAEDLGRDRIYVPATDMKRFHVQ
                     EADLAAPTAGASVRALVAFQAQRALDLLDEGAPLVGSVRGRLRLLLAGFVAGGRAAVRA
                     IAAADHDVLPGPPKPGTLQLLREAGVTLRGKG"
     gene            5874..6824
                     /gene="hpnD"
                     /locus_tag="IE148_RS32905"
                     /old_locus_tag="GCM10010287_66400"
     CDS             5874..6824
                     /EC_number="2.5.1.103"
                     /GO_function="GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006139892.1"
                     /locus_tag="IE148_RS32905"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_66400"
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="WP_189366839.1"
                     /sec_met_domain="phytoene_synt (E-value: 2.4e-59, bitscore:
                     190.8, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PT_phytoene_like (E-value: 7.9e-73,
                     bitscore: 235.3, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRTVESQPSASAPVLAAYSYCETVTGQQARNFAYGIRLLPTPKR
                     RAMSALYAFSRRVDDIGDGPLPEDVKAARLEDTRALLTRIREGAVEEDDTDPVAVALSH
                     AAAHFPIPLGGLDELIDGVQMDLRGEAYETWDDLKVYCRCVAGSIGRLSLGVFGTEPGA
                     HGVERASEYADTLGLALQLTNILRDVREDAENGRTYLPADDLAKFGCADGFAGPTPPQG
                     SDFAGLVHFEVRRARALFAEGYRLLPMLDRRSGACVAAMAGIYRRLLDRIERDPEAVLR
                     GRVSLPGREKAYVAVRGLSGLDTRHVARRTVGRRA"
     gene            6824..6943
                     /locus_tag="IE148_RS34420"
     CDS             6824..6943
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039420.4"
                     /locus_tag="IE148_RS34420"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6380 family protein"
                     /protein_id="WP_351036934.1"
                     /transl_table=11
                     /translation="MDTPYPDGKRRATLRRAPASLTETAGRAPVAPGRPGEGA"
     gene            6940..8370
                     /gene="hpnE"
                     /locus_tag="IE148_RS32910"
                     /old_locus_tag="GCM10010287_66410"
     CDS             6940..8370
                     /EC_number="1.17.8.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133315.1"
                     /locus_tag="IE148_RS32910"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_66410"
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="WP_086703596.1"
                     /sec_met_domain="DAO (E-value: 1.6e-11, bitscore: 34.3,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSHGTRSEGAPHTDGPVPPGRHAVVVGGGLAGTTAALALADAGVR
                     VTLLEGRPRLGGLAFSFKRGDLTVDNGQHVYLRCCTAYRWFLDRIGGAGLAPLQDRLDV
                     PVVDAARPEGRRLGRLRRDPLPVPLHLGRSLAAYPHLSLADRARVGRAALALKGLDLDD
                     PALDEQDFGSWLAAHGQSPRAVEALWDLVGVATLNAVAGDSSLALAAMVFKTGLLSDPG
                     AADIGWARVPLGDLHDRLAREALDAAGVRTATRTRVSSVRADGDGGWSVDIPGETLRAD
                     AVVLAVPQREAYDLLPAGALDDPDRLLGIDTAPILNVHVVYDRAVLTRPFFAALGTPVQ
                     WVFDRTHASGLRHGQYLALSQSVAHDDIDAPVADLRERYLPELERLLPRARGAEVLDFF
                     VTRERTATFVPAPGVGRLRPGARTEAPGLYLAGAWTATGWPATMESAVRSGVGAADAAL
                     GALGRPRPRHLFALEEAA"
     gene            8442..9506
                     /locus_tag="IE148_RS32915"
                     /old_locus_tag="GCM10010287_66420"
     CDS             8442..9506
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003972234.1"
                     /locus_tag="IE148_RS32915"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_66420"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_189366843.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 5.6e-61, bitscore:
                     196.1, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPPASQAARRTAVDVSALLERGRTLATPVLRAAVDRLAPPMDTVA
                     AYHFGWIDAQGRPADGDGGKAVRPALAVLSAEVTGAAPETGVPGAVAVELVHNFSLLHD
                     DLMDGDEQRRHRDTVWKVHGPAQAILVGDALFALANEVLLELGTVEAGRAARRLTRASR
                     ALIDGQAQDISYEHRDRVSVEECLEMEGNKTGALLACASSIGAVLGGADEPTADALERY
                     GHHLGLAFQAVDDLLGIWGDPESTGKQTWSDLRQRKKSLPVVAALAADGPAATRLGEIL
                     AADAKSSDFANFSEEEFAARAALIEEAGGREWTAEEARRQHTIAIEALDVVDMPDRVRE
                     QFTALADFVVVRKR"
     gene            9623..11662
                     /gene="shc"
                     /locus_tag="IE148_RS32920"
                     /old_locus_tag="GCM10010287_66430"
     CDS             9623..11662
                     /EC_number="5.4.99.17"
                     /GO_function="GO:0016866 - intramolecular transferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="shc"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T2TS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011031166.1"
                     /locus_tag="IE148_RS32920"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_66430"
                     /product="squalene--hopene cyclase"
                     /protein_id="WP_189366840.1"
                     /sec_met_domain="T2TS (E-value: 7.2e-153, bitscore: 500.0,
                     seeds: 58, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTATTDGSTGAASPSRLTAASEHDTDSPGAAGVPDIAARAVRRAT
                     DFLLSRQSDEGWWKGDLETNVTMDAEDLLLRQFLGIRDEDTTRAAALFIRGEQREDGTW
                     ATFHGGPGDLSATVEAYVALRLAGDAPEAEHMAKASAWIREQGGIAASRVFTRIWLALF
                     GWWKWEDLPELPPELIWFPTWVPLNIYDFGCWARQTIVPLTIVSAVRPVRPAPFPLDEL
                     HTDPANPNPPRPLAPASTWDGAFQRLDRGLHKLRRAVPRRLRRAAMNTAARWIIERQEN
                     DGCWGGIQPPAVYSVIALHLLGYDLNHPVMRAGLESLDRFAVWREDGARMIEACQSPVW
                     DTCLATIALADAGLPADHPALVKAADWMLGEQVVRPGDWSVRRPHLPPGGWAFEFHNDN
                     YPDIDDTAEVVLALRRVRHHDPERLDNAVGRGVRWNLGMQSRNGAWGAFDVDNTSPFPN
                     RLPFCDFGEVIDPPSADVTAHVVEMLAVEGLAQDPRTRRGVEWLLAEQELDGSWFGRWG
                     VNYIYGTGSVVPALTAAGLPTSHPAIRRAVAWLEKVQNDDGGWGEDLRSYRYVKEWSGK
                     GASTASQTAWALMALLAAGERDSEAVERGVAWLADTQREDGSWDEPYFTGTGFPWDFSI
                     NYHLYRQVFPLTALGRYVHGEPFAAKPAEVPAGSAQALADAKGS"
     gene            11662..12303
                     /locus_tag="IE148_RS32925"
                     /old_locus_tag="GCM10010287_66440"
     CDS             11662..12303
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431357.1"
                     /locus_tag="IE148_RS32925"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_66440"
                     /product="1-hydroxy-2-methyl-2-butenyl 4-diphosphate
                     reductase"
                     /protein_id="WP_189366841.1"
                     /transl_table=11
                     /translation="MSGRPAPTPLLIACALGIEHLALRRRERTTPGGPVTVLRTGMGPA
                     AAERSVTRVLADPALHGAAVLATGFCAGLAPGMHPGDLVVAEETRDPRGTVPCVGTELL
                     VKELMRAVPGRTVHTGPLTGSDHVVRGHERSDLLATGAIAVDMESAATLLSAVRAAERP
                     VAAVRVVVDAPEHELVRIGTVRGGISAFRVLRSVLPAFYEWHRSLLLPRR"
     gene            12309..13331
                     /gene="hpnH"
                     /locus_tag="IE148_RS32930"
                     /old_locus_tag="GCM10010287_66450"
     CDS             12309..13331
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386169.1"
                     /locus_tag="IE148_RS32930"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_66450"
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="WP_048460834.1"
                     /sec_met_domain="PF04055 (E-value: 3.5e-19, bitscore: 59.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 1.7e-10, bitscore: 30.8,
                     seeds: 32, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAMPLRQSIKVATYLAEQKLRRRDKFPLIVELEPLFACNLKCEGC
                     GKIQHPAGVLKQRMPVAQAVGAVLESGAPMVSIAGGEPLMHPQIDEIARQLVARRKYVF
                     LCTNALLMRKKMDKFTPSPYFAFAVHIDGLRERHDESVAKEGVFDEAVEAIKEAKRRGF
                     RVTTNSTFFNTDTPQTVVEVLNYLNDDLKVDEMMISPAYAYEKAPDQEHFLGVEQTREL
                     FRKAFSGGNRRRWRLNHSPLFLDFLEGKVDFPCTAWAIPNYSLFGWQRPCYLMSDGYVP
                     TYRELIEDTDWDKYGRGKDPRCANCMAHCGYEPTAVLATMGSLKESLRAMRETVSSNRE
                     "
     gene            13338..>13473
                     /locus_tag="IE148_RS32935"
                     /pseudo=""
     CDS             13338..>13473
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007501661.1"
                     /locus_tag="IE148_RS32935"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="4-hydroxy-3-methylbut-2-en-1-yl diphosphate
                     synthase"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTAVSLGLPEVPATLAVRRRSRQIQVGSVAVGGDAPVSVQSMTTT
                     "
ORIGIN
        1 cgacgtggcg ccgatcggcg acgtccggtg ggtcgagatc gacaaccacg acgacctcgc
       61 ccgcggacgg gagatcgcgt gccagtactg acccggctca tcccctcgcc ggtcgtcgtc
      121 gacatccgtc ccggcgccct cgacgacctg gcctgcgtcc tcgcggacga gcgcatctcg
      181 cagtccggcc ggctcgccat cgccgtcagc ggcggctccg gcgcccggct gcgcgaccgc
      241 gtcgcgccgt cgctgcccgg cgccgactgg ttcgaggtcg gcggcggcac catcgacgac
      301 gcggtccggc tggccggcgc catccagggc ggccgttacg acgcggtcgt cggcctcggc
      361 ggcgggaaga tcatcgactg cgccaagttc gcctcggcgc gcgtcggcct gcccctggtc
      421 gcggtcgcca ccaatctggc gcacgacggc ctgtgctcgc cggtcgccac cctcgacaac
      481 gacgcgggac gcggctccta cggcgtgccc aacccgatcg cggtcgtgat cgacctcaac
      541 gtcatccgcg aggccccggt ccggttcgtc cgctcgggca tcggcgacgt cgtctccaac
      601 atctccgccg tcgccgactg ggagctgtcc caccgcgtca acggcgagaa ggtcgacgga
      661 ctcgccgccg ccatggcccg ccaggccggc gaggccgtgc tccggcaccc gggcggtgtc
      721 gacgacgaca gcttcctcca ggtgctcgcc gaggccctgg tgctcaccgg catctcgatg
      781 tccgtgtccg gcgactcccg gccctcctcc ggcgcctgcc acgagatcaa ccacgccttc
      841 gacctgctct acccgcagcg cgccgccgcc cacggcgagc agtgcggact gggcgccgcc
      901 ttcgcgatgt acctgcgcgg cgcgcacgag gagtcggcgg ccatggccga ggtgctgcgc
      961 cggcacgggc tgccggtgct ccccgaggag atcgggttct ccgtcgacga gttcgtccgc
     1021 gtcgtcgagt acgcccccga gacccgcccc ggccgcttca ccatcctcga acatctcgcc
     1081 ctgaccaccg aacagatcaa ggacgtctac gccgactatg tcaaggccat cggtagctga
     1141 actccgtccg gtcgtgcacc ccgcgggggt caaggaccgg cgcagcggtg agcactggat
     1201 gggacgcctc tacatgcgcg aggtgtccct gcgggtcgac cgctacctgg tgaacacccg
     1261 ggtcaccccc aaccagctca cgtatctgat gaccctctgc ggtgtcctcg cggcccccgc
     1321 gctcctggtg cccggcatca cgggggcggt cctcggggtg gtcgcggtcc agctgtacct
     1381 gctgctcgac tgcgtcgacg gcgagatcgc ccgatggcgc aagcagtact ccctcgccgg
     1441 cgtctacatg gaccgcgtcg gcgcctacct gaccgacgcc gccgtgctgg tcggcctcgg
     1501 cctgcgcgcc gccgacctgt ggggcacagg ccgcatcgac tggctgtggg ccttcctcgg
     1561 gaccctcgcc gcgctgggcg cgatactcat caaggcggag accgacctgg tcggtgtcgc
     1621 ccgtcaccag aacggcatgc ccccggtgaa ggagtccgcc tccgagccgc gctcctccgg
     1681 catggcgctc gcccgcaagg ccgccgccgc cctgaagttc caccggctga tcctgggcat
     1741 agaggcgtcc ctgctgatcc tgctcctcgc gatcctcgac tccgcccggg gcgacctgtt
     1801 cttcacccgc ctcggcaccg ccgtcctcgc gggcatcgcc ctgctgcaga cgctgctgca
     1861 cctcgtgtcc gtcctcgtct cgagcaggct gaagtgaacg cgggcatgaa ggtcggcgcg
     1921 gtgatcatca ccatgggtaa ccgccccgac gaactgcggg ccctgctcga ctccgtcgcc
     1981 aagcaggacg gcgaccccgt cgaggtcgtg gtcgtcggca acggctcgcc cgtgcccgac
     2041 gtccccgagg gcgtcaagac cgtcgagctg cccgagaacc tgggcatccc cggcgggcgc
     2101 aacgtcggca tcgaggcctt cgggcccagc ggccgggacg tcgacatcct gctcttcctc
     2161 gacgacgacg gcctgctcgc gcacaccgac accgccgagc tgtgccggaa ggcgttcgcc
     2221 gaggacccct cgctcggcat catcagcttc cgcatcgccg acccggacac cggcgtcacc
     2281 cagcgccggc acgtgccccg gctgcgtgcc tccgacccga tgcgctcctc ccgcgtgacc
     2341 acgttcctcg gcggcgccaa cgcggtgcgc acccaggtcc tcgccgaggt cggcggactg
     2401 ccggacgagt tcttctacgc ccacgaggag accgacctgg cctggcgtgc cctcgacgcc
     2461 ggctggatga tcgactaccg gtcggacatg gtgctgtacc acccgacgac cgccccctcg
     2521 cggcacgcgg tgtaccaccg catggtggcc cgcaaccgcg tctggctcgc ccggcgcaac
     2581 ctgcccgtcc cgctcgtccc ggtgtacctg ggcgtctggc tgctcctcac cctcctgcgc
     2641 cgcccctcgg gctccgccct caaggcgtgg ttcggcggct tcaaggaggg ctggacctcg
     2701 ccctgcgggc cccgtcgccc catgaagtgg cgtacggtgt ggcggctgac ccgactgggc
     2761 cggcccccgg tgatctgaca agcttcgagt ccggggatcc ggcccctgcc cggtccccgg
     2821 ctcctggcag gcccgcgcgg gccccgcaca tcgaggacga aagttaatcc acccgtgagt
     2881 gagactacgc acgacggcac ggtcgcggtc ggcgcgcccg tgccgcccga cgagggcctt
     2941 ccggcggccg agctggccgc gaagtacggg ctgtccgtca gcggtgcccg cccgtccctc
     3001 ggcgagtacg tccgtcagct gtggggacgg cggcacttca tcctggcctt ctcgcaggcc
     3061 aagctgaccg cccagtacag caaggcgaag ctgggccagc tgtggcaggt nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnngc cgctgctgaa cgcggccgtg tacttcttca tcttcggcct
     3181 gctgctcggc gcccggcgcg gcatcccgag cgacgtctac gtgccgttcc tcgtgacggg
     3241 cgtgttcgtg ttcaccttca cgcagagctc ggtgctggcc ggtgtccggg cgatctcggg
     3301 gaacctgggg ctggtgcggg cgctgcactt cccgcgggcg tcgctgccga tctcgttcgc
     3361 gctccagcag ctccagcagc tgctgttctc gatgatcgtc ctggtcgtca tcatggtggc
     3421 gttccggagc tatccggacc tgtcgtggct gctggtggtg ccggcactgg ggctgcagtt
     3481 cgtcttcaac accggcctcg cgctgatctt cgcgcggatg ggcagcaaga cccccgacct
     3541 ggcgcagctg atgccgttcg tgctgcggac ctggatgtac gcgtccggcg tgatgttctc
     3601 catcccggcc atgctggagg acaagaacgt gccgtcctgg gtggcggacg tgctccagtg
     3661 gaacccggcc gcggtctaca tggacctggt gcgatacgcg ctgatcgaca agtacgactc
     3721 gacctatctg ccgccgcaca tctgggcgtt cggactgggc tgggcggtgc tcgccggcgt
     3781 ggtcggcttc gtctacttct ggaaggcaga ggagaggtac ggccgtggct gacgacaaga
     3841 cgggcgcccg ggtgcccacc gtcatcgcgg acgacctgca catcgtctac cgcgtgaacg
     3901 gcacccggtc gggcaaggga agcgccaccg cggcgctcag ccggatcctc aagcgcggcg
     3961 aggagcgggg cgtgcgcaag gtgcacgccg tgcgcggggt gtccttcgtg gcctaccgcg
     4021 gcgaggccat cggcctgatc gggtccaacg gctccggcaa gtcgaccctg ctgcgcgcca
     4081 tcgccggact gctgcccgcc gagcgcggca aggtctacac cgacggccag ccgtccctgc
     4141 tcggcgtgaa cgcggccctg atgaacgacc tcaccggcga gcgcaacatc atcctcggcg
     4201 ggctcgcgat gggcatgtcc cgcgaggaga tcaaggcgcg ctaccaggac atcgtcgact
     4261 tctccgggat caacgagaag ggcgacttca tcaccctgcc gatgcgcacc tactcctccg
     4321 gcatggccgc ccgtctgcgc ttctccatcg ccgccgccaa ggaccacgac gtcctgatga
     4381 tcgacgaggc gctggccacc ggcgaccgga agttccagaa gcgctccgag gcccgcatcc
     4441 gcgagctgcg caaggaggcc gggacggtgt tcctggtcag ccacaacaac aagtccatcc
     4501 gcgacacctg cgaccgggtg ctgtggctgg agcgcggcga gctgcgcatg gacgggccga
     4561 cggacgaggt gctccgcgag tacgagaagt tcaccggcaa gtagcccgtt tcgcccaagt
     4621 ccccgccgga acggtccggc ggggtcccgc gtctgcaaaa gatgcgtcaa ctccccgcgc
     4681 acggcggaag cctgacgccg gacgatgtgt tgtcgtggag cacggaagac ccccgtcgag
     4741 gaacggcgcg ttgtacaacg taagctgtac cggtccgcga aaggcggcaa tcagggccat
     4801 aatgcccgac accattcctc ggcgccgtcg ggtggacgcc tgggcggcgt gtccgaaata
     4861 gtgtgaattg ggtcagcagt gtagaacggg agatgtgacg gcaatggctt cggagactcc
     4921 ccggctgaac tcagcatgcg ccgtcctctc cccgggcagt ccgcgatgac ggccgccggc
     4981 cggacgcgcc ccggcgacct cgaacgggcc accctcgaca aggccgccgc cgagaacttc
     5041 ccggtggcgc ccttcttcct gcccagggcc tggcgcgacg acctcatggc cgtctacggc
     5101 tacgcccgtc tggtcgacga catcggcgac ggcgacctcg cccccggcgg ggccgacgcc
     5161 cgcgccctcg gtgtccccga ggacaaggcc ggcgaccgcc tcaccctgct ggacgccctg
     5221 gaagccgatc tccaccgcgt gttcgacccg aagggcccgg cgccccgtca ccccctgctg
     5281 ctccgcctgg agcccacggt ccgccgccgc gcgctgaccc ccgagccgtt cctcgggctg
     5341 atcgccgcga accgccagga ccagctggtc acgcgctacg agacctacga cgacctgctc
     5401 gcctactgcg agctgtcggc caacccggtc ggccgcctcg tgctcgccgt caccgggacc
     5461 gccacaccgg agcgcatccg ccgctccgac gcgatctgca cggccctgca gatcgtcgag
     5521 caccttcagg acgtcgccga ggacctcggc cgcgaccgca tctacgtgcc cgccacggac
     5581 atgaaacgtt tccacgtcca ggaggccgac ctcgccgcgc ccaccgccgg cgcctcggtg
     5641 cgcgcgctcg tcgcgttcca ggcgcagcgc gccctcgacc tgctggacga gggcgcaccc
     5701 ctggtgggca gcgtccgggg ccggctcaga ctcctgctcg cggggttcgt ggcgggagga
     5761 agggcggccg tccgcgcgat cgcggccgcc gaccacgatg tacttcccgg cccgcccaag
     5821 cccggcacgc tgcagctgct gcgtgaggcg ggcgtgactc tgcgaggaaa ggggtgatcc
     5881 ggaccgtgga gtcccagcca tccgcgtccg caccggtact cgccgcctac agctactgcg
     5941 agaccgtcac cggccagcag gcacgcaact tcgcctacgg catccggctg ctgccgacgc
     6001 ccaagcggcg cgccatgtcg gccctgtacg cgttctcccg gcgggtcgac gacatcggcg
     6061 acggaccgct ccccgaggac gtcaaggccg cccgtctcga ggacacccgc gcgctgctca
     6121 cccggatccg cgagggcgcg gtcgaggagg acgacaccga cccggtcgcg gtcgccctct
     6181 cccacgccgc ggcgcacttc ccgatcccgc tcggcggcct ggacgaactc atcgacggcg
     6241 tccagatgga cctgcgcggc gaggcgtacg agacctggga cgacctgaag gtctactgcc
     6301 gctgcgtcgc gggctccatc ggacggctct cgctcggcgt gttcggcacc gagcccggcg
     6361 cgcatggtgt ggaacgcgcc tccgagtacg ccgacacgct gggcctggcg ctccagctca
     6421 ccaacatcct gcgcgacgtc cgcgaggacg ccgagaacgg gcggacctac ctgcccgccg
     6481 acgacctcgc caagttcggc tgcgccgacg gcttcgccgg accgacaccg ccccagggct
     6541 cggacttcgc gggcctggtg cacttcgagg tgcgcagggc ccgcgccctc ttcgccgagg
     6601 gctaccggct gctccccatg ctcgaccggc gcagcggcgc ctgcgtcgcc gccatggccg
     6661 gcatctaccg ccgtctcctg gaccgcatcg agcgcgaccc cgaggccgtg ctgcgcggcc
     6721 gggtctccct tcccggacgg gagaaggcgt acgtggcggt gcgcggcctg tccggtctcg
     6781 acacccggca cgtcgcccgg cgcaccgtcg ggaggcgcgc ctgatggaca ccccgtaccc
     6841 ggacggaaag cggcgggcaa ccctccgccg cgcccccgcg tccctcaccg agacggccgg
     6901 ccgcgcacct gtcgcgcccg gcaggccggg ggagggcgca tgagccacgg cacacgctcc
     6961 gagggggccc cgcacacgga cggcccggtc ccgccgggac gccatgccgt cgtggtcggc
     7021 ggcggactgg ccggcaccac cgccgccctc gccctcgccg acgccggagt gcgcgtcacc
     7081 ctgctcgaag gccggccccg cctgggcgga ctcgccttct ccttcaagcg cggcgacctg
     7141 accgtcgaca acggccagca cgtctacctg cgctgctgca ccgcctaccg ctggttcctc
     7201 gaccgcatcg ggggagccgg tctcgccccg ctgcaggacc gcctcgacgt gcccgtggtc
     7261 gacgcggccc gccccgaggg caggcggctc ggacggctgc ggcgcgaccc tctgccggtg
     7321 cctctccacc tcggccgcag cctcgccgcg tacccccacc tgtcgctcgc cgaccgggcg
     7381 agggtgggcc gtgccgcgct cgcgctgaag ggcctcgacc tcgacgatcc cgccctggac
     7441 gagcaggact tcggcagctg gctcgccgcc cacggccagt ccccgcgcgc cgtcgaagca
     7501 ctgtgggacc tggtcggcgt cgccaccctc aacgccgtcg ccggcgactc gtcgctggcg
     7561 ctcgccgcga tggtgttcaa gaccggcctg ctgtccgacc ccggcgccgc cgacatcggc
     7621 tgggcccgtg tgccgctcgg cgacctgcac gaccggctcg cccgtgaggc cctcgacgcc
     7681 gccggcgtac gcaccgcgac ccgcacccgc gtcagctccg tgcgcgccga cggtgacggc
     7741 ggctggagcg tcgacatccc cggcgagacg ctccgcgccg acgccgtcgt cctggccgtg
     7801 ccccagcgcg aggcgtacga cctgctgccc gcgggcgcgc tcgacgaccc ggaccggctg
     7861 ctcggcatcg acaccgcccc catcctcaac gtgcacgtcg tctacgaccg cgcggtgctc
     7921 acccggccct tcttcgccgc gctcggcacc cccgtgcagt gggtcttcga ccgcacccac
     7981 gcttccggac tgcgccacgg ccagtacctg gcgctgtccc agtcggtcgc ccacgacgac
     8041 atcgacgcgc ccgtggccga cctgcgcgag cggtacctgc ccgaactgga gcggctgctg
     8101 ccgcgcgccc gcggcgccga ggtcctcgac ttcttcgtca cccgggagcg caccgcgacc
     8161 ttcgttcccg cccccggcgt cggacgcctc aggcccggcg cccgcaccga ggcacccggc
     8221 ctctacctgg ccggagcgtg gaccgccacc gggtggcccg cgaccatgga gagtgcggtc
     8281 cgcagcggcg tcggcgcggc ggacgccgcg ctcggcgccc tcggccggcc ccgcccgcgc
     8341 cacctcttcg cgctcgagga ggccgcctga tgctgcagcg gagcggcgcg gcgggtcccc
     8401 gcacccccgg taccgcaaca agaggagaga ctgtgcccac tgtgcccccg gcctcgcagg
     8461 ccgctcgaag gaccgcggtg gacgtgtccg cgctcctgga gcgcggccgg accctggcca
     8521 cgccggtact gcgggcggcc gtcgaccgcc tggcacctcc catggacacg gtcgccgcct
     8581 accacttcgg ctggatcgac gcccagggcc ggcccgcgga cggcgacggc ggcaaggccg
     8641 tgcgccccgc cctcgccgtg ctgtccgccg aggtcaccgg cgcggctccg gagaccggcg
     8701 tgcccggcgc ggtcgccgtc gagctggtcc acaacttctc gctgctgcac gacgacctga
     8761 tggacggcga cgaacagcgc cggcaccgcg acaccgtctg gaaggtgcac ggccccgccc
     8821 aggccatcct ggtcggtgac gccctgttcg ccctggccaa cgaggtgctg ctggagctcg
     8881 gcaccgtcga ggccggccgc gccgcacgcc gcctcacccg ggccagccgc gcgctgatcg
     8941 acggtcaggc ccaggacatc tcgtacgagc accgcgaccg ggtcagcgtc gaggagtgcc
     9001 tggagatgga gggcaacaag accggcgccc tgctcgcctg cgccagctcc atcggcgcgg
     9061 tcctcggcgg cgccgacgag cccaccgccg acgccctgga gcgctacggc caccacctgg
     9121 gcctcgcctt ccaggccgtc gacgacctgc tcggcatctg gggcgacccg gagtccaccg
     9181 gcaagcagac ctggagcgac ctgcgccagc gcaagaagtc cctcccggtc gtggccgcgc
     9241 tcgccgcgga cggcccggcc gccacccggc tcggcgagat cctcgccgcc gacgccaaga
     9301 gcagcgactt cgcgaacttc tccgaggagg agttcgcggc ccgtgccgcc ctcatcgagg
     9361 aggccggcgg ccgtgagtgg accgccgagg aggcacgacg ccagcacacc atcgccatcg
     9421 aggccctcga cgtcgtggac atgcccgacc gggtgcggga acagttcacg gcgctcgcgg
     9481 acttcgtcgt cgtacgaaag agatgatcac tatcggtcga atagccctcg cgtagtcgcc
     9541 ggaccggtgg ggcgcacccc caccggccga cggcggaccc acagcagacg caccacttgc
     9601 aggactgcac gaaggggaag ccatgacagc gacgaccgac ggaagcaccg gggcggcctc
     9661 gccgtcccgg ctcaccgcgg ccagcgaaca cgacaccgac agccccgggg cggccggggt
     9721 acccgacatc gccgcccgcg ccgtacggcg cgccaccgac ttcctgctgt cccgtcagag
     9781 cgacgagggc tggtggaagg gcgacctcga gaccaacgtc acgatggacg ccgaggacct
     9841 gctgctgcgt cagttcctcg gcatccgcga cgaggacacc acccgggccg ccgccctgtt
     9901 catccgcggg gagcagcgcg aggacggcac ctgggccacc ttccacggcg gccccggcga
     9961 tctctccgcc accgtcgagg cgtacgtcgc gctgcggctg gcgggcgacg cccccgaagc
    10021 cgagcacatg gcgaaggcct cggcctggat ccgggagcag ggcggcatcg ccgcgtcccg
    10081 ggtcttcacc cgcatctggc tggccctgtt cggctggtgg aagtgggagg acctgcccga
    10141 actgccgccc gagctgatct ggttccccac ctgggtgccg ctcaacatct acgacttcgg
    10201 ctgctgggcg cggcagacca tcgtgccgct caccatcgtc tccgccgtgc ggccggtgcg
    10261 ccccgccccg ttcccgctgg acgagctgca caccgacccg gcgaacccca accctccccg
    10321 gccgctcgcc cccgcgagca cctgggacgg cgccttccag cggctcgaca ggggcctgca
    10381 caagctgcgc cgggccgtac cgcgccggct gcgccgggcc gcgatgaaca ccgccgcccg
    10441 ctggatcatc gaacggcagg agaacgacgg ctgctggggc ggcatccagc cccccgccgt
    10501 gtactcggtc atcgccctgc acctgctcgg ctacgacctg aaccacccgg tgatgcgtgc
    10561 cggtctggag tccctggacc gcttcgccgt gtggcgcgag gacggcgccc ggatgatcga
    10621 ggcctgccag tccccggtgt gggacacctg cctcgccacc atcgcgctgg ccgacgcggg
    10681 cctgcccgcc gaccacccgg ccctggtcaa ggccgccgac tggatgctgg gcgagcaggt
    10741 ggtgcggccc ggcgactggt ccgtgcgccg gccccatctc ccgccgggcg gctgggcgtt
    10801 cgagttccac aacgacaact accccgacat cgacgacacc gccgaggtgg tcctcgcgct
    10861 gcgccgggtc agacaccacg acccggagcg gctggacaac gccgtcgggc gcggggtgcg
    10921 ctggaacctc ggcatgcagt cgaggaacgg agcctggggc gccttcgacg tcgacaacac
    10981 cagcccgttc cccaaccggc tgccgttctg cgacttcggc gaggtcatcg acccgccgtc
    11041 ggccgacgtc accgcgcacg tcgtggagat gctcgccgtc gaagggctcg cccaggaccc
    11101 gcgcacccgg cgcggcgtgg agtggctcct cgccgaacag gaactggacg gctcgtggtt
    11161 cggccgctgg ggcgtcaact acatctacgg caccggctcc gtggtccccg ccctgaccgc
    11221 ggccggcctc cccacgtccc acccggcgat ccggcgggcc gtggcctggc tggagaaggt
    11281 gcagaacgac gacggcggct ggggcgagga cctgcgctcc taccgctacg tcaaggagtg
    11341 gagcggcaag ggcgcctcca ccgcctccca gaccgcctgg gcgctgatgg cgctgctcgc
    11401 ggcgggggag cgggactccg aggcggtcga acggggcgtc gcctggctgg cggacaccca
    11461 gcgggaggac ggctcctggg acgagccgta cttcaccggc accggcttcc cgtgggactt
    11521 ctccatcaac taccacctct accggcaggt gttcccgctc accgcgctcg gccggtacgt
    11581 ccacggcgag ccgttcgccg cgaagccggc cgaggtgccg gcgggctccg cgcaggcgct
    11641 cgccgacgcc aagggcagct gatgagcggc cggcccgccc cgaccccgct gctgatcgcc
    11701 tgcgcgctcg gcatcgaaca cctcgcgctg cgcaggcggg agcgcaccac cccgggcggg
    11761 ccggtcaccg tgctgcgcac cggcatggga ccggcggcgg ccgagcggtc cgtcacccgg
    11821 gtgctcgccg acccggcgct gcacggggcc gccgtcctcg ccaccggctt ctgcgcgggc
    11881 ctcgcccccg gcatgcaccc cggcgacctg gtcgtcgccg aggagacccg ggacccacgc
    11941 ggcaccgtgc cgtgcgtcgg caccgagctg ctggtgaagg agctgatgcg cgcggtgccc
    12001 ggacgcaccg tgcacaccgg ccccctcacc ggatccgacc acgtcgtacg cggccacgaa
    12061 cgctccgacc tgctcgccac cggcgcgatc gcggtggaca tggagtcggc cgccacgctc
    12121 ctgagcgccg tccgcgcggc cgagcgcccg gttgcggccg tccgggtggt cgtggacgct
    12181 cctgaacacg aactcgtccg catcggcacg gtccgcggtg gaatatcggc tttccgtgtc
    12241 cttcgttccg tactcccggc tttctatgaa tggcaccgtt ccttgctgct ccccaggagg
    12301 tgagccagat ggccatgccg ctgcgtcagt ccatcaaggt cgctacatac ttggccgaac
    12361 agaagctccg caggcgggac aagttcccgc tcatcgtcga actggaaccg ctcttcgcct
    12421 gcaacctcaa gtgcgaggga tgcggcaaga tccagcatcc ggccggtgtg ctcaagcagc
    12481 gcatgcccgt cgcgcaggcc gtcggcgccg tactggagtc cggggcgccc atggtgtcga
    12541 tcgccggcgg cgagcccctg atgcaccccc agatcgacga gatcgcgcgt cagctggtgg
    12601 cccgccggaa gtacgtcttc ctgtgcacca acgcgctgct gatgcgcaag aagatggaca
    12661 agttcacccc gtcgccgtat ttcgccttcg cggtgcacat cgacgggctg cgggagcggc
    12721 acgacgagtc cgtggccaag gagggcgtgt tcgacgaggc cgtggaggcc atcaaggagg
    12781 ccaagcggcg cggcttccgg gtcaccacca actcgacgtt cttcaacacc gacaccccgc
    12841 agaccgtcgt cgaggtgctc aactacctca acgacgacct caaggtcgac gagatgatga
    12901 tctcgcccgc ctacgcctac gagaaggcgc ccgaccagga gcacttcctg ggtgtggagc
    12961 agacccggga gctgttcagg aaggccttct ccggcggcaa ccgccggcgc tggcggctca
    13021 accactcccc gctgttcctc gacttcctcg agggcaaggt ggacttcccg tgcaccgcct
    13081 gggcgatccc caactactcc ctgttcggct ggcagcgccc ctgctacctg atgagcgacg
    13141 gctacgtgcc gacctaccgg gagctcatcg aggacaccga ctgggacaag tacggccgcg
    13201 gcaaggaccc gcgctgcgcc aactgcatgg cgcactgcgg ttacgagccc accgccgtcc
    13261 tcgccaccat gggctccttg aaggaatccc tgcgcgccat gcgcgagacc gtctcgtcga
    13321 acagggagtg atcacagatg accgcagtct ccctgggcct ccccgaggtc ccggccacgc
    13381 tcgccgtgcg ccggaggagc cggcagatcc aggtcggctc cgtggcggtg ggcggggacg
    13441 ccccggtgtc ggtgcagtcg atgacgacga cgc
//
