LOCUS       NZ_BMTZ01000024        10399 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces variabilis strain JCM 4422 sequence24, whole genome
            shotgun sequence.
ACCESSION   NZ_BMTZ01000024
VERSION     NZ_BMTZ01000024.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces variabilis
  ORGANISM  Streptomyces variabilis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces griseoincarnatus
            group.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 90570)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 156X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649935.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:37:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,743
            CDSs (total)                      :: 6,668
            Genes (coding)                    :: 6,496
            CDSs (with protein)               :: 6,496
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 61 of 172
            Pseudo Genes (incomplete)         :: 147 of 172
            Pseudo Genes (internal stop)      :: 11 of 172
            Pseudo Genes (multiple problems)  :: 42 of 172
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:23:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 76188
            Orig. end                         :: 86587
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMTZ01000024.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    20..34
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 19.36"
                     /tool="antismash"
     misc_feature    complement(364..378)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
     gene            1383..1991
                     /locus_tag="IE148_RS27835"
                     /old_locus_tag="GCM10010287_56210"
     CDS             1383..1991
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="IE148_RS27835"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_56210"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_048458472.1"
                     /transl_table=11
                     /translation="MDLDIPELPFPLRTYGPDGSWSYEDGVLTGWAGPRQDRFVPPTGE
                     SLESAADAPRLLGAPEGDFQLIARVTVGFAGSFDAGVLYVHVGERAWAKLCLEYSPDVP
                     TVCTVVTRGHSDDANSFTVDGKTVWLRVSRTGRALAFHASRDGKQWIFVRLFTLGDEKE
                     TGAALVGFATQSPMGEGCVVTFDHIEYRLHWPPDLRDGS"
     gene            2095..2835
                     /locus_tag="IE148_RS27840"
                     /old_locus_tag="GCM10010287_56220"
     CDS             2095..2835
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019522167.1"
                     /locus_tag="IE148_RS27840"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_56220"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_189366362.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALASFPHRDGVEVVHRSFELDP
                     GRAKDDVEPVLTMLTKKYGMSQEQAEAGEDNLGAQAAAEGLPYRTRGRDHGGTFDMHRL
                     LHFAREHGGQERLLDLLYRANFAEERSLFGDDERLVELAGAAGLDPEAARAVLADPEAY
                     AAEVRADEQDAARLGARGVPFFVLDRKYGVSGAQPVDVFTRALTQAWGERSPLTLVDGG
                     TDAEACGPDGCAVPERENKRGQNA"
     gene            2949..4001
                     /locus_tag="IE148_RS27845"
                     /old_locus_tag="GCM10010287_56230"
     CDS             2949..4001
                     /NRPS_PKS="Domain: Aminotran_5 (52-344). E-value: 7.5e-24.
                     Score: 76.2. Matches aSDomain:
                     nrpspksdomains_IE148_RS27845_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019522166.1"
                     /locus_tag="IE148_RS27845"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_56230"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_086701398.1"
                     /sec_met_domain="Aminotran_5 (E-value: 3.7e-20, bitscore:
                     64.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFESLVRAEFTPKNTYLNTASNALLPARAVAALDAAVRLRAEG
                     RPLDPLFADVEACRAAYARLADVPAERVAAGATVALFSSLVAASLPPGADVLTADDDFT
                     SVLNPFHARGDLKVRSAPLEGLADAVRPGTALVAVSAAQSADGRIADLPALREAARSQG
                     ARTYVDFSQAAGWLPVEADAYDYTVAITFKWLLGVHGAAFLTVPEDFGDLVPVQAGWTA
                     GEDPWGSCYGPVAELAHSARRFDSTPALFSYAGLRASLTLLEELGVDAVRAHDLALADR
                     FRAGLASLGHEPLPAPGSPIVSVPGLGRLQPELSRAGIEVSNRAGNLRASFHLYNTAAD
                     VDRLLDALSG"
     aSDomain        3105..3980
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE148_RS27845_Aminotran_5.1"
                     /evalue="7.50E-24"
                     /label="IE148_RS27845_Aminotran_5.1"
                     /locus_tag="IE148_RS27845"
                     /protein_end="344"
                     /protein_start="52"
                     /score="76.2"
                     /tool="antismash"
                     /translation="ADVEACRAAYARLADVPAERVAAGATVALFSSLVAASLPPGADVL
                     TADDDFTSVLNPFHARGDLKVRSAPLEGLADAVRPGTALVAVSAAQSADGRIADLPALR
                     EAARSQGARTYVDFSQAAGWLPVEADAYDYTVAITFKWLLGVHGAAFLTVPEDFGDLVP
                     VQAGWTAGEDPWGSCYGPVAELAHSARRFDSTPALFSYAGLRASLTLLEELGVDAVRAH
                     DLALADRFRAGLASLGHEPLPAPGSPIVSVPGLGRLQPELSRAGIEVSNRAGNLRASFH
                     LYNTAADVDRL"
     gene            complement(4098..4994)
                     /gene="thpD"
                     /locus_tag="IE148_RS27850"
                     /old_locus_tag="GCM10010287_56240"
     CDS             complement(4098..4994)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448161.1"
                     /locus_tag="IE148_RS27850"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_56240"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_048458475.1"
                     /sec_met_domain="PhyH (E-value: 3e-41, bitscore: 134.5,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTTTRVTDLYPTRGATEVVTPRQDPVVWGSPDTPGPVSAGDLQA
                     LDRDGFLAVDQLIGPDEVEVYKRELDRLVDDPAIRADERSIVEPKSQEIRSVFEVHKIS
                     EVFANLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPN
                     MRTISVSIALTENYDTNGGLMIMPGSHKTFLGCAGATPKDNYKKSLQMQDAGTPSDEAL
                     TKMASEYGIKLFTGKAGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENTAVEPFAA
                     PIRRPEFIGARDFTPVK"
     gene            complement(5001..5399)
                     /locus_tag="IE148_RS27855"
                     /old_locus_tag="GCM10010287_56250"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037840.1"
                     /locus_tag="IE148_RS27855"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_56250"
                     /product="ectoine synthase"
                     /protein_id="WP_004932953.1"
                     /sec_met_domain="ectoine_synt (E-value: 4.7e-56, bitscore:
                     180.6, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKEIEGTDRHVKSASGTWESKRIVLAKEKVGFSVHETILY
                     AGTETDMWYANHIEAVVCTKGDAELTDRETGKTYHITPGTMYLLDGHERHTLKVKEDFH
                     CICVFNPPVTGREDHDENGVYPLLTEEV"
     gene            complement(5432..6703)
                     /gene="ectB"
                     /locus_tag="IE148_RS27860"
                     /old_locus_tag="GCM10010287_56260"
     CDS             complement(5432..6703)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 8.3e-72.
                     Score: 234.0. Matches aSDomain:
                     nrpspksdomains_IE148_RS27860_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007448159.1"
                     /locus_tag="IE148_RS27860"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_56260"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_048458477.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.6e-77, bitscore:
                     253.4, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPTVFDRAHGSRMYDEDGHEY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLERDGVTHGLDMSTTAKRAFLESFQNYILRPRD
                     LPYKVMFPGPTGTNAVESALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GVPLVHGTPMPFDNYFDGQVPDFLWFERILEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALSELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGITPDIVTVSKSISGYGLPMA
                     LTLFKPELDVWEPGEHNGTFRGNNPAFVTATAALEAYWADGSAMEKQTRGRGEQVDQAM
                     IAITEENLADVKEYRGRGLVWGLEFHDKERAGRVAKRAFELGLLIETSGPESEVVKLLP
                     ALTITPDELDEGLRVLARAVRETA"
     aSDomain        complement(5606..6619)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE148_RS27860_Aminotran_3.1"
                     /evalue="8.30E-72"
                     /label="IE148_RS27860_Aminotran_3.1"
                     /locus_tag="IE148_RS27860"
                     /protein_end="366"
                     /protein_start="28"
                     /score="234.0"
                     /tool="antismash"
                     /translation="FDRAHGSRMYDEDGHEYLDFFAGAGSLNYGHNNPVLKRALIDYLE
                     RDGVTHGLDMSTTAKRAFLESFQNYILRPRDLPYKVMFPGPTGTNAVESALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGVPLVHGTPMPFDNYFDGQVPDFLWFER
                     ILEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALSELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGITPDIVTVSKSISGYGLPMALTLFKPELDVWEPGEHNGTFRGNNPAFV
                     TATAALEAYWADGSAMEKQTRGRGEQVDQAMIAITEENLADVKEYRGRGLVWGLEFH"
     gene            complement(6772..7278)
                     /gene="ectA"
                     /locus_tag="IE148_RS27865"
                     /old_locus_tag="GCM10010287_56270"
     CDS             complement(6772..7278)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004002246.1"
                     /locus_tag="IE148_RS27865"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_56270"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_086701397.1"
                     /transl_table=11
                     /translation="MTAAQADLQIDRPTVADGAVLWRIARDSKVLDLNSSYSYLLWCRD
                     FAATSAVARDERGEAVGFITGYLRPDSPRTLLVWQVAVDEAYRGRGLAAAMLDALAART
                     AGEHGVDTVETTVAPGNTASERLFTAFAERHGALLEREVLFDTPDFPDGPHDPEVLHRI
                     GPFAL"
     misc_feature    complement(7303..7315)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(7435..7450)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    7445..7460
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: medium,
                     score: 17.62"
                     /tool="antismash"
     misc_feature    complement(7445..7462)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     gene            complement(7632..7841)
                     /locus_tag="IE148_RS27870"
                     /old_locus_tag="GCM10010287_56280"
     CDS             complement(7632..7841)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF016840.5"
                     /locus_tag="IE148_RS27870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_56280"
                     /product="DUF664 domain-containing protein"
                     /protein_id="WP_189366363.1"
                     /transl_table=11
                     /translation="MRGGGAAQARGRDLCAGRPLDGTGRIADGPPAGTEVSPRRVLIHV
                     IEEHARHNGHADLLRERIEGVTGA"
     gene            complement(7892..8992)
                     /locus_tag="IE148_RS27875"
                     /old_locus_tag="GCM10010287_56290"
     CDS             complement(7892..8992)
                     /NRPS_PKS="Domain: Aminotran_5 (19-343). E-value: 1.3e-28.
                     Score: 91.9. Matches aSDomain:
                     nrpspksdomains_IE148_RS27875_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019522160.1"
                     /locus_tag="IE148_RS27875"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_56290"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_189366364.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.7e-23, bitscore:
                     75.6, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNLLLDLPPLTAARFASIEDRVARLLGTGQDVLITQGEALLPLE
                     GAIRAAAGPGTTALNVITGPYGQTFGDWLRDCGATVIDLTVPFHTAVTAEQVRDAFAEH
                     PEIDFVSLVHAEAATGNTNPVAAIGEVVREHGALFYLDAVASVGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPNAPRRSYLSLLDWKERWIDGGRKTLLHAP
                     AQLEMLALEACVERIEAEGPEAVMARHASAAAATRAGATALGGGIAPYVHDAADAAPVA
                     TTLRAPDGTTASDLVARALAADPGLPLAAGGGALAREMIRVNHYGPEATRGTVRACLAA
                     LATALTDAGVSVDLPAGLAAAERAWV"
     aSDomain        complement(7964..8935)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE148_RS27875_Aminotran_5.1"
                     /evalue="1.30E-28"
                     /label="IE148_RS27875_Aminotran_5.1"
                     /locus_tag="IE148_RS27875"
                     /protein_end="343"
                     /protein_start="19"
                     /score="91.9"
                     /tool="antismash"
                     /translation="EDRVARLLGTGQDVLITQGEALLPLEGAIRAAAGPGTTALNVITG
                     PYGQTFGDWLRDCGATVIDLTVPFHTAVTAEQVRDAFAEHPEIDFVSLVHAEAATGNTN
                     PVAAIGEVVREHGALFYLDAVASVGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSAVSVS
                     ERAWARMAANPNAPRRSYLSLLDWKERWIDGGRKTLLHAPAQLEMLALEACVERIEAEG
                     PEAVMARHASAAAATRAGATALGGGIAPYVHDAADAAPVATTLRAPDGTTASDLVARAL
                     AADPGLPLAAGGGALAREMIRVNHYGPEATRGTVRACLAALAT"
     gene            9097..9945
                     /locus_tag="IE148_RS27880"
                     /old_locus_tag="GCM10010287_56300"
     CDS             9097..9945
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020121392.1"
                     /locus_tag="IE148_RS27880"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_56300"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_086701392.1"
                     /transl_table=11
                     /translation="MKTHPGRRTRVLVATTATAGMVLLAACTSTDDGGGGSTTAGGVEL
                     VKAGQLTTCTHLPYPPFQSEIDGKVQGFDVALIDLVADDLGVKQQILDTPFENFKTGAF
                     LNSGECDLAAAGMTITDERKKNVDFSDPYFNATQAVLVAKDSGVGSLADVKSENVELGA
                     QAQTTGEDYVKGEGFDPVSFESSDAVLNGLRTGQVKAVVIDYPVVQGWLKDEANADAFT
                     VAEQIDTGEQYGFTVKKGNKALQEAVNKALADAKADGTYEKLYEQWIGPYDESAASPAA
                     S"
     misc_feature    complement(10058..10073)
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: medium, score: 25.44"
                     /tool="antismash"
     misc_feature    10310..10324
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.26"
                     /tool="antismash"
ORIGIN
        1 gcgttggtga ggccctcctg gacgatgcgg tacaccgcgt ggtccgcggc cggggcgagc
       61 ggccgcgcat cgccccggcg gcgcagctcc accggcgcgc ccgcgccccg cgacgacgcg
      121 accagcgcgt ccaccccggc ggcgccccgg gcgggcgtcg cccccgccgc ctccggttcg
      181 gggccgggag cgggcgtggt cggcccctcg gtgtccaccc ggaggatgcc gacggcctcg
      241 cgcagctccc gcatcgccga cctggcggcc tcccgcagga tgcccactcc ctcccgctgc
      301 cggccgccga gctccgggtc cacctcgagc gcccccgcgt gcaccgcgat cagggcgagc
      361 tggtggccga ggctgtcgtg catgtcctgg gcgatccgct gccgctccag cagccgcgcc
      421 tgccgtgcca ccatcgcctg ttcgcgcacc agttgggtgt tgtgccggcg cagtgcctcc
      481 agcaggtcac ggcgttgcgc gcggtaccgg gaggcgagcc ccggaacgac cgccagcacc
      541 aggtaggggc ccgtgcccag ggccgccgcc atcagcagcg acagctccag cgcgtcctcg
      601 gccccgtccc cgagcagacc gcccgcgacg tacagcacca gcccggcggc gtacgcgccg
      661 accgcgcgcc aggggcgctt gatgcgactg cccgccgacc agctcgcgta catgagcagc
      721 gggccggcgc cgagaaccga gccggccagg gcggccgaga cgacgagcac ggtggcgggc
      781 agcgaccgcc ggaccagcga gagcagaccg acgagcaggg ccaccgccca gcccttggcg
      841 gtggatccgc tctccatggc gtacatcggg cccgcgatgc ccgccagcac caggagcagc
      901 acggtctccc cggcgatccg gccccgcgac cagggggagg cggtggccgg ccgggacagc
      961 gagcggcggg cggggcgaga ggtctgcgct gtgtccaccc gcccgaccct aggggcccgc
     1021 accggggcgc cggcatcctc cctccgtagc ggtcccgagc gacgaaagtg aacggcgtcc
     1081 gtcaacgtcc gtatcaccac cggccgttgt ctaccgcgac ttccgaggac tgcgcccgca
     1141 cggcccgccc cgcgacgacg gcggagccgg cgaggacctc cgccggcggc acaccccacc
     1201 gacaccggga gcacagcagt ggacgcgacg ggcccctggg ccggccggcg cttcaccgtc
     1261 acacggaagg ccaccccgag gaccgaccgg tacctcgtca ccggggccgc cctggacacc
     1321 ttccgggacc gctgaccggg atccgccacg cgcggcgggc ccgctcctgg gacaatcccc
     1381 ccatggacct ggacataccc gaactcccct tcccgctgcg gacctacggc cccgacggct
     1441 cctggtccta cgaggacgga gtcctcaccg gatgggcggg gccccggcag gaccgcttcg
     1501 tgccgcccac cggcgagtcc ctggagtccg ccgccgacgc gccgcggctg ctcggcgcgc
     1561 ccgagggcga cttccagctg atcgcccggg tcacggtcgg cttcgccggc tccttcgacg
     1621 ccggggtgct ctacgtccac gtcggcgagc gggcgtgggc caagctgtgc ctggagtact
     1681 ccccggacgt gcccaccgtc tgcacggtcg tcacccgggg ccactccgac gacgccaact
     1741 ccttcaccgt ggacggcaag acggtctggc tgcgggtgag ccgcaccggg cgcgccctcg
     1801 ccttccacgc ctcgcgcgac ggcaagcagt ggatcttcgt ccggctgttc accctcggcg
     1861 acgagaagga gacgggagcg gccctggtcg ggttcgcgac ccagtcgccg atgggggagg
     1921 ggtgcgtggt gaccttcgac cacatcgagt accggctgca ctggccgccg gacctgcgcg
     1981 acggaagctg agcgaggtca ggggcccgtc ggggaatgcc ggcggatgct tgaacgttca
     2041 tccggtcgcg gaggacgtct ccgcagccgt gccgaccctg gagagagccc gcccatgcgc
     2101 gtcgagatct ggagcgacat cgcctgcccc tggtgctatg tcggaaaagc ccgcttcgag
     2161 aaggcgctgg cctccttccc gcaccgcgac ggggtcgagg tggtccaccg ctccttcgag
     2221 ctcgacccgg gccgcgccaa ggacgacgtc gagcccgtgc tcacgatgct caccaagaag
     2281 tacggcatga gccaggagca ggccgaggcg ggcgaggaca acctgggcgc ccaggccgcc
     2341 gccgaggggc tgccctaccg cacccggggc cgcgaccacg gcggcacctt cgacatgcac
     2401 cgcctgctgc acttcgcccg cgagcacggc ggccaggagc ggctgctcga cctgctctac
     2461 cgggccaact tcgccgagga gcgctccctg ttcggcgacg acgagcggct cgtcgaactc
     2521 gccggcgccg ccggcctcga ccccgaggcc gcccgcgcgg tgctcgccga cccggaggcg
     2581 tacgccgccg aggtccgcgc cgacgagcag gacgccgccc ggctcggcgc gcgcggggtg
     2641 ccgttcttcg tgctcgaccg gaagtacggc gtctccggcg cccagcccgt cgacgtcttc
     2701 acccgggcgc tcacccaggc ctggggcgag aggtccccgc tgaccctcgt cgacgggggc
     2761 acggacgccg aggcctgcgg cccggacggc tgcgcggtgc ccgagcggga gaacaagcgg
     2821 gggcagaacg cgtaagggtg gaatgcgcag gccggcgggt acgtacagcg gacgtataag
     2881 cattccttag cggtaccccg cagacatatg tgatggacgg gggcccggag gggtcgcaga
     2941 gtggtcccat ggagaccttc gagagcctcg tccgtgccga gttcaccccg aagaacacct
     3001 acctgaacac cgcgagcaac gccctgctgc ccgcccgggc cgtggccgcg ctcgacgcgg
     3061 ccgtccggct gcgcgcggaa gggcgcccgc tcgatccgct gttcgccgac gtcgaggcgt
     3121 gccgtgccgc gtacgcccgg ctggcggacg tgcccgccga gcgggtggcg gccggggcga
     3181 ccgtcgccct gttctcctcg ctggtcgccg cgtccctgcc gccgggcgcc gacgtcctca
     3241 ccgccgacga cgacttcacc tccgtgctga accccttcca cgcgcgcggc gacctgaagg
     3301 tgcggtccgc gccgctggag ggcctggccg acgctgtgcg gccgggcacc gcgctggtcg
     3361 cggtcagcgc cgcgcagtcc gccgacggcc ggatcgccga cctgcccgcg ctgcgcgagg
     3421 cggcccgctc ccagggggcg cgcacctacg tcgacttctc ccaggcggcg ggctggctgc
     3481 cggtggaggc cgacgcgtac gactacaccg tcgccatcac cttcaagtgg ctgctgggcg
     3541 tgcacggcgc ggccttcctc accgtccccg aggacttcgg cgacctggtc ccggtgcagg
     3601 ccggctggac ggcgggggag gacccgtggg gcagctgcta cggccccgtc gccgaactcg
     3661 cccacagcgc acggcggttc gactccaccc ccgcgctgtt cagctacgcg ggcctgcggg
     3721 cgtccctcac gctcctcgag gagctcggcg tggacgccgt ccgcgcccac gacctcgccc
     3781 tcgccgaccg cttccgcgcc ggactcgcct cgctgggcca tgagcccctg cccgcgcccg
     3841 gctcgcccat cgtgtccgtg ccggggctgg ggcggcttca gccggagctg tcgcgggcgg
     3901 gcatcgaggt gtcgaaccgg gccggcaacc tgcgggcgtc gttccacctc tacaacacgg
     3961 ccgccgacgt ggaccggctg ctcgacgcgc tgtccggctg agcgcacggc agcgggccgg
     4021 tgcctccttg tcccacacga ggaggcccgg cccgcagtcg gtgaggcggt gcgcacacgt
     4081 caccgccgta cgtgtcccta cttcacgggg gtgaagtccc gcgcgccgat gaactcgggg
     4141 cggcggatcg gagccgcgaa cggctcgacc gccgtgttct cgacgctgtt gaacacgatg
     4201 aacacgttgc tgcgcgggta cggcgtgatg ttgtcgccgg agccgtgcat gcagttgcag
     4261 tcgaaccagg tcgccgagcc ggccttgccg gtgaacagct tgatgccgta ctccgacgcc
     4321 atcttggtca gcgcctcgtc ggacggcgtc cccgcgtcct gcatctgcag ggacttcttg
     4381 tagttgtcct tcggggtggc gcccgcgcag ccgaggaacg tcttgtgcga ccccggcatg
     4441 atcatcagac cgccgttggt gtcgtagttc tcggtgagcg cgatcgagac cgatatggtg
     4501 cgcatgttcg gcaggccgtc ctcggcgtgc caggtctcga agtccgagtg ccagtagaag
     4561 ccgctcgcgc cgaagccggg cttgacgttg atccgcgact ggtggacgta cacgtccgag
     4621 ccgaggatct gccgggcgcg cccgacgacc cgctcgtcgc gcaccaggtt cgcgaagacc
     4681 tcgctgatct tgtgcacctc gaagaccgag cggatctcct gggacttcgg ctcgacgatc
     4741 gagcgctcgt ccgcgcggat cgccgggtcg tccaccagcc ggtccagctc gcgcttgtag
     4801 acctcgacct cgtccgggcc gatcagctgg tcgacggcca ggaagccgtc gcggtccagc
     4861 gcctggaggt cacccgcgga gacggggcca ggggtgtccg gggaacccca gacgaccggg
     4921 tcctggcgcg gggtgaccac ctcggtggcg ccgcgggtcg ggtagagatc ggtgacgcgc
     4981 gtggtcgtgg tcacggtgga tcacacctcc tcggtgagca gcggatagac gccgttctcg
     5041 tcgtggtcct cccgtccggt cacgggcggg ttgaagacac agatgcagtg gaagtcctcc
     5101 ttgaccttga gcgtgtgccg ctcgtggccg tcgaggaggt acatggtgcc gggcgtgatg
     5161 tggtaggtct tcccggtctc gcggtcggtc agctcggcgt cgcccttggt gcagacgacg
     5221 gcctcgatgt ggttcgcgta ccacatgtcc gtttccgtac ccgcgtacag gatcgtctcg
     5281 tgcacggaga agccgacctt ctccttggcg aggacgatgc gcttgctctc ccacgtgccg
     5341 gacgccgatt tcacgtgccg gtcggtgcct tcgatctcct tgaacgaacg gacgatcacg
     5401 gtgttgcgat gcctcctcgg ctggatgtgt ttcaggcggt ttcgcggacg gcgcgggcca
     5461 ggaccctgag gccctcgtcc agctcgtccg gggtgatggt cagggcgggg agcagcttga
     5521 cgacctcgct ctccgggccg gacgtctcga tcagcagacc gagctcgaag gcgcgcttgg
     5581 cgacccggcc ggcgcgctcc ttgtcgtgga actccaggcc ccacacgagg ccgcggccgc
     5641 ggtactcctt gacgtcggcg aggttctcct cggtgatggc gatcatcgcc tgatccacct
     5701 gctcgccgcg tccccgggtc tgcttctcca tcgcggagcc gtccgcccag tacgcctcaa
     5761 gggcggcggt ggccgtcacg aacgcggggt tgttgccgcg gaacgtgccg ttgtgctcgc
     5821 ccggctccca gacgtccagc tccggcttga acagggtcag cgccatgggc aggccgtagc
     5881 cgctgatcga cttggagacg gtgacgatgt ccggcgtgat gcccgcctcc tcgaaggaga
     5941 agaacgcgcc ggtgcggccg cagcccatct ggatgtcgtc gacgatgagc agcatgtcct
     6001 ggcgctcgca cagctcggac agggcgcgca gccactcggg ccgggccacg ttgatgccgc
     6061 cctcgccctg cacggtctcg acgatcacgg cggccggctt gttgaggccc gagccctggt
     6121 cctccaggat gcgctcgaac cacaggaagt ccgggacctg gccgtcgaag tagttgtcga
     6181 acggcatggg cgtgccgtgc accagcggga caccggcacc ggcccgcttg aaggcgttgc
     6241 cggtcacggc gagcgagccc agtgacatgc cgtggaaggc gttggtgaac gacacgacgg
     6301 actcgcggcc cttcaccttg cgcgccagct tcagcgcgga ctccacggcg ttggtgcccg
     6361 tcgggcccgg gaacatgacc ttgtacggca ggtcacgcgg gcgcaggatg tagttctgga
     6421 aggattccag gaacgcgcgc ttggccgtcg tcgacatgtc gaggccgtgc gtgacgccgt
     6481 cccgctccag gtagtcgatc agggcgcgtt tcaggacggg gttgttgtgg ccgtagttga
     6541 gtgaaccggc tccggcgaag aagtcgaggt actcgtggcc gtcctcgtcg tacatgcggc
     6601 tgccgtgcgc acggtcgaag acggtgggcc agccgcggca gtagctgcgc acctcggact
     6661 cgagggtctc gaagacgctg aggtcgggct gggtgatggt cacgacgaat cgctcctcgg
     6721 tggcgtggga agtcggaggg agtcggaaga tcggggggtc gggcccgcgc gtcagagcgc
     6781 gaagggcccg atgcggtgca ggacctcggg gtcgtggggt ccgtcgggga agtccggggt
     6841 gtcgaagagc acctcgcgct ccagcagggc gccgtggcgc tccgcgaacg cggtgaacag
     6901 gcgctcggag gcggtgttgc cgggtgcgac ggtggtctcc acggtgtcca cgccgtgctc
     6961 cccggcggtc cgggcggcca gcgcgtcgag catggcggcg gccaggccgc ggccgcggta
     7021 cgcctcgtcg acggcgacct gccagaccag cagggtgcgc gggctgtccg ggcggaggta
     7081 gccggtgatg aagccgaccg cctctccgcg ctcgtcgcga gcgacggccg aggtggcggc
     7141 gaagtcacgg caccacagca gatagctgta cgacgagttc aggtcgagga ccttcgagtc
     7201 cctggctatc cgccacagca cggctccgtc cgccacggtc gggcggtcga tttgcaggtc
     7261 tgcttgtgcg gcagtcatgc gaattgaact taccgaggta aattcaaaat tgcatcgtcc
     7321 tgcggggtta cgtacgagca cgtcatgtgt tatcgcgcgt acgggtgtgt ccgcgcgaga
     7381 tgccggtgaa atgtcccgtt tcgcacggga ggaagtggtg caaatggggc gcgctgtgga
     7441 tccggtcaca tctgtgtaac tctcgtgacc acacccggaa acgcccggtg gccactcctt
     7501 ggattggggc gtttaggctc cggaagaccg ggcagaagaa gacgggaagc tgttccgcag
     7561 agtaataggg aaataagccg gagaatgtcg cgggagtatg tgtgcgaggg atttcggtaa
     7621 tccctttgaa ttcacgcccc ggtcacccct tcgatgcgct cccggagaag atcggcgtgg
     7681 ccgttgtggc gcgcatgctc ctcgatcaca tggatcagca cccggcgcgg gctgacctcc
     7741 gtgccggccg gcggcccgtc ggcgatgcgt ccggtgccgt ccagggggcg cccggcgcac
     7801 aggtcccgtc cccgggcctg cgcggcaccc ccgccccgca tcggcaccgg cgcccaagaa
     7861 gctcgtccag ggccgtgggg ccccgcctcg cttacaccca ggcccgctcc gccgccgcca
     7921 gccccgcggg caggtcgacc gacacccccg cgtccgtcag ggccgtagcc agagccgcca
     7981 agcacgcccg gaccgtgcca cgggtggcct cgggcccgta gtgattgacg cggatcatct
     8041 cgcgggccag ggcgccgccc cccgctgcga gaggcaggcc ggggtcggcc gccaacgccc
     8101 gcgcgaccag atccgacgcg gtcgtaccgt ccggcgcccg cagcgtcgtg gcgaccggcg
     8161 ccgcgtccgc cgcgtcgtgg acgtacggcg cgatcccccc gccgagcgcc gtcgcacccg
     8221 cacgggtcgc ggcggcagcc gaggcgtgcc gcgccatcac cgcctccggt ccctccgcct
     8281 cgatccgctc cacgcacgcc tcgagcgcga gcatctcgag ctgcgccgga gcgtgcagca
     8341 gcgtcttgcg gccaccgtcg atccaccgct ccttccagtc caggagggag aggtaggagc
     8401 ggcggggcgc gttcgggttg gccgccatgc gggcccaggc acgctcgctc accgagaccg
     8461 ccgacacccc ggcgggcccg cccatcgcct tctgcgcccc gatgacgcac aggtccacgc
     8521 cccacgcgtc cggcagcacc ggctccgcgc ccaccgaggc gaccgcgtcc aggtagaaca
     8581 gcgccccgtg ctcccgcacg acctcgccga tcgcggcgac cgggttggtg ttgccggtgg
     8641 cggcctcggc gtgcaccagc gacacgaagt cgatctccgg gtgctcggcg aacgcgtccc
     8701 gcacctgctc ggcggtcacc gccgtgtgga acggcacggt caggtcgatg acggtggctc
     8761 cgcagtcccg cagccagtcg ccgaaggtct gcccgtaggg gccggtgatg acgttcagcg
     8821 cggtcgtccc gggcccggcc gccgcacgga tcgcgccctc cagcggcagc agcgcctcac
     8881 cctgggtgat cagcacgtcc tgcccggtgc ccagcagccg cgccacccgg tcctcgatcg
     8941 aggcgaagcg cgcggcggtc agcgggggca ggtccaggag gaggttcgtc acggctgtgc
     9001 tctctttgct catgcttcac ggcagcgcgg gtaggtacga cgacgtcgag ggtaaacctt
     9061 cggcggacgg ttccgtttcc cccacaggag gcctccgtga agacgcatcc cggccgcagg
     9121 acccgggtcc tggttgccac caccgcgacg gccgggatgg tgctcctcgc cgcctgcacg
     9181 tccaccgacg acggaggcgg agggtccacg accgccggcg gggtcgagct cgtcaaggcg
     9241 gggcagctca ccacctgcac ccacctgccg tacccgccgt tccagtcgga gatcgacggc
     9301 aaggtgcagg gcttcgacgt cgccctgatc gacctggtcg ccgacgacct cggcgtgaag
     9361 cagcagatcc tcgacacgcc gttcgagaac ttcaagaccg gcgccttcct caactccggc
     9421 gagtgcgacc tggccgcggc cggcatgacc atcaccgacg agcggaagaa gaacgtcgac
     9481 ttctccgacc cgtacttcaa cgccacccag gccgtcctcg tcgccaagga cagcggcgtc
     9541 ggctccctcg ccgacgtgaa gtcggagaac gtcgagctgg gcgcccaggc acagaccacc
     9601 ggcgaggact acgtcaaggg cgagggcttc gacccggtct ccttcgagtc ctccgacgcc
     9661 gtcctcaacg gcctgcgcac cggccaggtc aaggccgtcg tcatcgacta ccccgtcgtc
     9721 cagggctggc tgaaggacga ggccaacgcg gacgccttca ccgtcgccga gcagatcgac
     9781 accggcgagc agtacggctt caccgtgaag aagggcaaca aggccctcca ggaggccgtg
     9841 aacaaggcgc tcgccgacgc caaggccgac ggcacgtacg agaagctgta cgagcagtgg
     9901 atcggcccgt acgacgagtc cgccgcctcc ccggccgcct cctgacccat ggccgacacg
     9961 gacgagacgc cgctccggcc caggcggaag ggcctgaccc ggcggcagaa gcgcagcgtg
    10021 tcgcgcggca tccagtacgc cgtgttcgtc gcggccgtga tcgccttcgg cgtcaccgcg
    10081 gactggggac ggctgcagaa ccagttcgcg caggtcgaga tcgccgagcg gatgttcccc
    10141 gaggtcatca cgctcgcgct gaagaacacc gtcctctaca cggtgaccgg cttcggcgtc
    10201 gggctcgcgc tcggcatgct gatcgcgctg atgcggctgt cgtccgtcgg gccctaccgc
    10261 tggctggccg gcatctacat cgagatcttc cgtggcctgc ccgccctgct gatcttcatc
    10321 ttcatcggcg tcgccgtccc gctcgccttc cccggcacgg agatcatcgg cgggacgtac
    10381 ggcaaggtcg ccctcgcgc
//
