LOCUS       NZ_BMTZ01000006        11380 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces variabilis strain JCM 4422 sequence06, whole genome
            shotgun sequence.
ACCESSION   NZ_BMTZ01000006
VERSION     NZ_BMTZ01000006.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces variabilis
  ORGANISM  Streptomyces variabilis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces griseoincarnatus
            group.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 416226)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 156X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649935.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:37:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,743
            CDSs (total)                      :: 6,668
            Genes (coding)                    :: 6,496
            CDSs (with protein)               :: 6,496
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 61 of 172
            Pseudo Genes (incomplete)         :: 147 of 172
            Pseudo Genes (internal stop)      :: 11 of 172
            Pseudo Genes (multiple problems)  :: 42 of 172
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:23:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 234985
            Orig. end                         :: 246365
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMTZ01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11380
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11380
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11380
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6380](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..6380
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     gene            561..1568
                     /locus_tag="IE148_RS13560"
                     /old_locus_tag="GCM10010287_27420"
     CDS             561..1568
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019328328.1"
                     /locus_tag="IE148_RS13560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_27420"
                     /product="iron ABC transporter permease"
                     /protein_id="WP_189364788.1"
                     /transl_table=11
                     /translation="MTRRFTVLGALAVVCAAVELVAGRGMSPGVVWDVLNGGGDATERH
                     IVFQLRLPRLMVALGAGACLAVAGLVLQSALRNPLAGPEVTGVTPGAVLGAVTATALGL
                     AGWDSPLAVVLAACAGGCAGAALLWLLAGRGRGDPAQTAVHGVLVSAVLGGLTAMVLLV
                     EPGELGSVVQWLVGTTEGRVWEHWHLLWPWALVWGAAAWLLAGPLTLLRCGDDIALAAG
                     LSVRRARTLALMCAVALTAGAVAAVGALGFIGLLVPHLAVAVFGADLRRSLPGAALTGA
                     VVVCGADAVAQLSSRLLATALDSGRLTLPVGALTACLGAALLLLVVRRTPGRTF"
     gene            1589..2410
                     /locus_tag="IE148_RS13565"
                     /old_locus_tag="GCM10010287_27430"
     CDS             1589..2410
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004114.1"
                     /locus_tag="IE148_RS13565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_27430"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_189364789.1"
                     /transl_table=11
                     /translation="MNESVGIHVEGVHFGYPGRPVLRGVDLTVRPGELTALIGLNGCGK
                     STLLRLAAGLLEPDEGRVLLGEDDVTRLSRRATARKVALLHQSAPAVPGMTVGHLVRQG
                     RYAARGPLGMLREGDDPVVRRALRDVGVEDWADRDVDALSGGERQRVRLAMALAQDTRV
                     LLLDEPTTYLDLHHQLDVLQTVVRLREERGLTVVMVLHDLAHAARFAERIVALREGRVV
                     ADGAPKQVVTPGLLAEVLQVAGRVGEDPEGGWPVCYPDHPIAALEYENQIH"
     gene            2468..2581
                     /gene="amiA"
                     /locus_tag="IE148_RS33625"
     CDS             2468..2581
                     /codon_start=1
                     /gene="amiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004115.1"
                     /locus_tag="IE148_RS33625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="streptamidine family RiPP"
                     /protein_id="WP_240449383.1"
                     /transl_table=11
                     /translation="MENEQVFAPIADPGQLAHDSASHSNALVENPFEDTEE"
     gene            2672..3526
                     /locus_tag="IE148_RS13570"
                     /old_locus_tag="GCM10010287_27440"
     CDS             2672..3526
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048303.1"
                     /locus_tag="IE148_RS13570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_27440"
                     /product="hypothetical protein"
                     /protein_id="WP_189364790.1"
                     /transl_table=11
                     /translation="MSRSRLVPGAEVVAVPGRGLAVRTPAGEFFTVRTGDADEDALLSL
                     LTGETTAPEDEGTERVIRAFEEAGYLAEGPSRPEWPVARRDVRILGDPVLTEPLAVQLA
                     ALGARPRTTPAVTDPGSSPVGLEDLLDGHPSAVVWCLDGPVPDGLWDAADRLPEHGVAW
                     LRCHREGWQAYVEPVAAAPGDVTSAHVRSRRLAATPAHRELAAYWSGPRTSGAPVRLAG
                     PAATLLAALLADDLGRWATGASGPAGFPVRRRLRRVDLRTLTVTEHPVLPVPDVAPIPK
                     KDG"
     gene            3523..5004
                     /locus_tag="IE148_RS13575"
                     /old_locus_tag="GCM10010287_27450"
     CDS             3523..5004
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004117.1"
                     /locus_tag="IE148_RS13575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_27450"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_189364791.1"
                     /transl_table=11
                     /translation="MTAFSTTVEELATDVLLPAGHGPFPTVLVRTPYDRTRHEAEARGW
                     ARRGFAAVVQDVRGRFASPGEWHPYAHEAADGTAAVRWIRQQPWSDGRLVAAGASYAAH
                     CALVLALDAPRDARPDAVVAAVPALGLAETAREASGVERLLSRAGWWAAHGDRRDSDEN
                     ALNKALAEDPGLLARLPLTALPERLGRALPSWAGLWLQRDRGRLVARAAHAGPPLLAVG
                     GHHDHFTEETVALWRAWGGSSARLLLGPWGHPLTAAPGPDAGPAHRLNLGELYVRWARR
                     ALAGDLAPGRHGALALGGAGLWLPADAEGEPRAHRMRLLHGSAFTADPARPLRSDDLTV
                     PADGPPDRCLLVTPPSPRPLDLVGPVSVRLRATAHTPSADWAARLVVLPPDGTAERLAV
                     GVVRRAEPAGRETAFTVGLGRLARRLRAGTRLRLEIAGHHFPAHARNPHTGDDPMTAAR
                     LLASRREVHPDSVVLNLPVLPSRPAPTDPAEEILR"
     gene            5001..6380
                     /locus_tag="IE148_RS13580"
                     /old_locus_tag="GCM10010287_27460"
     CDS             5001..6380
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004118.1"
                     /locus_tag="IE148_RS13580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_27460"
                     /product="YcaO-like family protein"
                     /protein_id="WP_189364792.1"
                     /sec_met_domain="YcaO (E-value: 8.1e-50, bitscore: 164.7,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTALPLEALVDPVCGIVRKVKPVEHPAGAPPRYTALTAEVSDARR
                     HGLWPADRVSLGTTFGDPEQARVAAIAEGMERYCGNRVPPPGHPDAPLRATAAELTDKG
                     LRLYGPADLPAYAPWQYARAKFPYRPLTADTPALWTRGTERLPDGTPAEVWAPVSLTHL
                     NWRQGGLRELPRTHHLNYAGIATGQGLDDAVERGLLEIVERDALELWWHLDGPARGIDP
                     ASVPGLSDDLAGSALDVHIVEMHSEFAPCMAALVHDPRLGLYAAGFACKYDPAEAARKA
                     VLEAVHTWVFTQGLTGPDGWVFQAVEAGLLARGLYLDHRADGRYLDVCGEQFEHVRDLG
                     AHVQVWLDERMAVEARRFTEPALGTVSVAEVEPGSRERLENTLREGGHRVMTFDLTTED
                     VAETALRVARVLVSGLLPNAPAAFGYFGCPRLAEAAVARGWRATPPSAPEDYTLAPPPH
                     M"
     gene            6389..7522
                     /locus_tag="IE148_RS13585"
                     /old_locus_tag="GCM10010287_27470"
     CDS             6389..7522
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048308.1"
                     /locus_tag="IE148_RS13585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_27470"
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="WP_246538977.1"
                     /transl_table=11
                     /translation="MADHREDPQTGLVAALARARSPERPGPDTSAGAPVREWAGPPLPI
                     PQAGPAHLDLRTLLHLSLAASDTSGRLRPTPSAGALHPVDTELVVGTGCSLPPGRYGYD
                     PLRHRVHHAGPPVDGTPPGVTAALSVTAQRTVSHYGHRAWPLLLLDTGHAAAALLLAAR
                     VLGAGESDVRMDGRTENPLAVVRIRPPYGRSPRHSARTEDLPTPADLLARRSTPPPLVG
                     LPPRDALRAVLSTAERAGGGDLLRWCAAVGPPRPGLMQLGPEGTTLRRYASGEARPTLA
                     AWAAGQAWLADAGAVLLAHGCPADADATHIRRAHLRAGFAVHLAHVTALRHGLAARPVG
                     SWQQADLGAALGAAPGRDWVVHALALGTHHADEESST"
     gene            7519..9258
                     /locus_tag="IE148_RS13590"
                     /old_locus_tag="GCM10010287_27480"
     CDS             7519..9258
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004120.1"
                     /locus_tag="IE148_RS13590"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_27480"
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="WP_189364794.1"
                     /transl_table=11
                     /translation="MIVHPELRRAARHARRPLLAATLLQGAVTLTYLAQAVLLALALAG
                     LARGDTGRLPWLLAGVLAVVAARAELALWQRRTATRAGARVRVALRDELLAHLGRLGPA
                     YLTTARAGAVRTTLVDGVEGVDAYVSRYLPQLLVTLAVPPLVLAVLVVVEPRALLGLVP
                     ALLLALLGPRAWDRLLAARGKEHWDTYEGLGADYLEALQGMPALRAAGAVGRTRARLEE
                     RSAALHRATVAKLRVSLVDTGITDLAIQGGTAAAALLACWSAVTGSTTAAGTYLVLLLA
                     SECFRPVRDHAREWHAGYLGVSAADGLASLRTSVPAVPDTATAPAHWHTPPELSFHSVR
                     FTYDGADAPALDGVSFTARAGRTTAIVGPSGAGKSTLLALLLRHHDPQEGRITLDGRPT
                     TEYALDSLRQGIAVVSQETYLFHATIADNLRLARPDATDDELTRAARAAGIHDEIAALP
                     DGYATLLGERGATLSGGQRQRLALARALLSDAPVLVLDEATSSVDERREADIVRELLNE
                     VGGRTVLVVAHRLAAVRSADRIVVLDAGRVDAVGDHTGLVDAGGVYTRLVEAGRAHRGE
                     LAA"
     gene            9255..11048
                     /locus_tag="IE148_RS13595"
                     /old_locus_tag="GCM10010287_27490"
     CDS             9255..11048
                     /EC_number="7.-.-.-"
                     /GO_component="GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018470983.1"
                     /locus_tag="IE148_RS13595"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_27490"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_189364795.1"
                     /transl_table=11
                     /translation="MSTVTGPTAAGAGLPARGALRALLPALAAHRAMTARTCAAALLEQ
                     GSLVALVTLAAHTVGIAVIEGRAPTAGTVTALVALVLVRALMTWRGMDLSHDLAYRVLA
                     ALRVHVFDGLARSAPARVAGRRSGDLAATAMADVEALEFFYAHTTAQLLAAGTVLTGGS
                     VLLALVEPWLLVAVLPVAALLAAAPFADAGARTVRGGRTRTAVAALSADTVETVDGLRE
                     LLAFGALAERRRTLAERGRQVGDAQRAEATWEAVAAAVRDLLIVVAVLGVVAAAAQSVT
                     SGRLHGAWVPAAMALALSVLGPVAESARALSQAVALRAAAARVDAAVKAPALAPPPTAP
                     RPLPPGPLGVRLHRVRFDYGGTPVLDGVDLAVRPGRTLALVGASGAGKSTCAHLLARFW
                     DPSAGEVQLLPADGDPVDVRQVDDAELRRAVVLVGQDTPLFHGTLAENLRLAAPEADDR
                     ALAEAARLCGVDRIAPLDTLVGERGATLSGGQRARIALARALLAGPRVLVLDETTAHLD
                     NTGDAELATALRQESRTTILIAHRPATIRRADRIAVLEGGLITEEGTWEDLTSSPDSAL
                     NRILSDTALTGHGSSPREPDQ"
ORIGIN
        1 ctgtgcgcgc cggtgctgtc cgtgctgcgg ctgggcgacg aggacgcgga ggcgctcggt
       61 gtgcgagcgc agcgcgcacg gctggcagcg ctggccatcg ccgtggtgct gatcgccccg
      121 gtgacggccg tgtgcgggcc cgtcgcgtgg gtggggttcc tcgccccgca cctgagccgc
      181 tggttcgctc ccgcggcggg agcggtgtgc tggctcccct ggtccgcggc ctggggcgcg
      241 gtcgtcgtgg tggtcgccga tgtcccggcc aggctggcgc tggcacccgt ggagaccccc
      301 gccggcgcgt ggacggccct gctgggggtg ccggccggcg tcgcgctgat gcggtcgggc
      361 ggccgccgcc ggacggcccg gcacggacag gatgcttccg ccggtacgcg cgagcagtcg
      421 tccgagccgc ggaacacggc ttcgggtgtg acggacgaag tgcctcccgc cggagcatca
      481 catcacgcgc accggggcga acccgggccc ggtggcccgg cctcgccgcc tctcgggacg
      541 gtcggaacgg aggacgtacg gtgacccggc gtttcacggt cctcggagca ctggccgtcg
      601 tgtgcgccgc agtggagttg gtggccggac gcggcatgtc cccgggggtg gtctgggacg
      661 tcctcaacgg tggcggtgac gcgacggagc gccacatcgt gttccaactg cgcctgccga
      721 ggctgatggt ggccctcggc gcgggtgcct gtctggccgt ggcgggactg gtcctgcagt
      781 ccgcactgcg taatcccctg gccgggccgg aggtgacagg tgtgaccccg ggggcggtcc
      841 tcggcgccgt caccgcgacc gccctggggc tcgccggatg ggattcgccc ctggccgtgg
      901 tcctcgccgc ctgcgcgggc gggtgcgccg gagcagcgct gctgtggctg ctcgccggcc
      961 gcgggcgcgg cgaccccgcg cagaccgccg tgcacggggt gctggtgtcg gcggtgctcg
     1021 gcgggctcac cgccatggtg ctgctcgtgg aaccgggtga gctcggcagc gtcgtgcagt
     1081 ggctggtggg caccacggag ggccgggtgt gggagcactg gcacctgctg tggccgtggg
     1141 cgctcgtctg gggggcagcc gcctggctgc tggcggggcc gttgaccctg ctgcgctgcg
     1201 gtgacgacat cgccctcgcc gccggtctct ccgttcgccg ggcccggacc ctggccctga
     1261 tgtgcgcggt ggccctgacc gccggcgccg tggccgccgt cggcgcgctg ggcttcatcg
     1321 ggttgctcgt cccgcatctg gccgtggccg tcttcggtgc ggacctgcgc cggagccttc
     1381 ccggcgccgc cctgacaggc gcggtcgtgg tgtgcggggc ggacgcggtg gcgcagttgt
     1441 cctcgcggct gctggcgacg gcgctggact ccgggcggct cacgttgccg gtcggggcgc
     1501 tgaccgcctg cctgggtgcc gcgctgctgc tgctcgtcgt gcgccgcacg ccgggccgta
     1561 cgttctgacg tcgaatcgag gacagagcat gaacgagtcc gtggggatcc acgtcgaggg
     1621 ggttcacttc ggctaccccg gacgacccgt gctgcggggc gtcgacctga ccgtccggcc
     1681 gggcgaactg actgccctga tcggcctgaa cggctgcggc aagtcgaccc tgctgcggct
     1741 ggccgccggg ctgctggaac ccgacgaagg gcgcgtgctg ctcggcgagg acgatgtcac
     1801 gcggctttcc agacgcgcca cggcccggaa ggtggccttg ctgcaccagt cggctccggc
     1861 cgtaccgggc atgacggtgg gtcacctggt ccggcagggg cggtatgcgg cgcgcggccc
     1921 gctgggcatg ctgcgcgagg gcgacgaccc cgtcgtgcgc agggcgctgc gtgatgtcgg
     1981 ggtggaggac tgggccgacc gtgacgtcga cgcgctgtcc ggcggcgagc ggcaacgtgt
     2041 ccggctcgcg atggcgctcg cccaggacac cagggtcctg ctgctcgacg agccgaccac
     2101 gtacctggat ctgcaccacc agctcgacgt actgcagaca gtggtgcgtc tgcgggagga
     2161 acgcgggctc acggtggtga tggtgctgca cgacctggct cacgcagccc ggttcgccga
     2221 gcggatcgtc gccctgcggg agggccgtgt ggtcgccgac ggagcgccga agcaggtcgt
     2281 cacgccgggg ttgctggcgg aggttctgca ggtggcaggc cgggtcggtg aggatccgga
     2341 aggggggtgg cccgtgtgtt acccagatca ccccatcgca gctctagaat atgaaaatca
     2401 gattcattag agtgaggtta cggcgctcgt ccgaacgccg tatctccctg ctgatgaagg
     2461 agagttcatg gaaaacgagc aggtcttcgc gccgatcgcc gacccgggtc agctggctca
     2521 tgactcggcc tcccactcca acgcgctcgt cgagaacccc ttcgaggaca ccgaggagta
     2581 agcgagggtc tggccaccct cgaccgtggg cccgcccgat gctccgggcg ggcccacgcc
     2641 cgtccccgct tccgtcgaca ggaggaacac cgtgtcccgt agccgactcg tccccggtgc
     2701 cgaggtcgtc gccgtgcccg ggcgagggct tgccgtccgc acccccgcag gggagttctt
     2761 caccgtcagg acaggagacg cggacgagga cgccttgctc tccctgctca ccggggagac
     2821 cacggccccg gaggacgagg ggacggaacg cgtgatccgg gcgttcgaag aagccggtta
     2881 tctggcggaa gggccgtcgc gcccggagtg gcccgtcgct cgccgggatg tcaggatcct
     2941 cggcgacccg gtcctgaccg aaccgctcgc cgtgcaactg gccgccctgg gcgcgcggcc
     3001 ccggacgact ccggccgtga ccgaccccgg cagctcgccg gtgggcctcg aggacctgct
     3061 cgacggacat ccctccgcgg tcgtgtggtg cctggacgga cccgtgccgg acggcttgtg
     3121 ggacgccgcc gaccggctgc cggagcacgg tgtcgcgtgg ctgcgctgcc accgcgaggg
     3181 ctggcaggcg tacgtcgaac cggtggccgc cgctcccggg gacgtcacct cggcgcacgt
     3241 ccggtcccgc aggctcgccg ccacacccgc tcaccgcgaa ctggccgcgt actggagcgg
     3301 accgcgtacc tccggcgcgc cggtccggct cgccggaccc gccgccacac tgctcgcggc
     3361 gctgctcgcg gacgatctgg gccggtgggc caccggagcc tccggtccgg cgggcttccc
     3421 ggtgcggcgc cgactgcgac gggtggacct gcgcacgctg accgtcaccg agcaccccgt
     3481 gctgccggtg cccgacgtcg cgccgatacc gaagaaggac ggatgacggc gttcagcacg
     3541 actgtggagg aactcgccac cgacgtcctg ctcccggccg gccacggacc cttcccgacc
     3601 gtcctggtgc gcacccccta cgaccggacc cggcacgagg ccgaggcgcg aggctgggcc
     3661 cgccgcgggt tcgccgccgt cgtccaggac gtgcgggggc ggttcgcgtc gccgggggag
     3721 tggcacccgt acgcgcacga ggccgccgac ggaacagcgg ccgtccgctg gatccggcag
     3781 cagccgtgga gcgacgggcg gctggtggcc gccggcgcct cctacgccgc ccactgcgcc
     3841 ctcgtcctcg ccctggacgc gccccgggac gcccggccgg acgccgtcgt cgcggccgtg
     3901 cccgccctcg gcctggcgga gaccgcccgc gaggcgtccg gcgtggaacg gctgctttcc
     3961 cgcgccggat ggtgggccgc ccacggcgac cggcgcgact ccgacgagaa tgcgctgaac
     4021 aaggccctcg ccgaggaccc cggcctgctc gcccgcctgc cgttgaccgc cctgcccgag
     4081 cggctgggac gcgccctgcc ctcgtgggcg gggttgtggc tccagcgcga ccgcgggcgc
     4141 ctcgtggcgc gggcggcgca cgccgggccg cccctgctcg cggtgggcgg ccatcacgac
     4201 cacttcaccg aggagaccgt cgctctgtgg cgcgcctggg gcggatcctc ggcgcgtctg
     4261 ctgctcgggc cctgggggca ccctctgacc gctgctccgg ggccggacgc gggacccgcc
     4321 caccggctga acctcggcga gctgtacgtg cgctgggccc gccgcgccct ggccggcgac
     4381 ctcgccccgg gccgccacgg cgcactggcg ctcggtggcg caggcctgtg gctgcccgcc
     4441 gacgccgagg gagaaccgcg tgcccaccgc atgcgcctgc tgcacggctc cgccttcacc
     4501 gccgaccccg cccggcccct tcgctccgac gacctgaccg tccccgccga cggcccgccc
     4561 gaccgctgcc tgctggtcac accgccgtcg ccgcgtccgc tcgacctcgt cggccccgtc
     4621 agtgtgcggc tgcgggccac cgctcacaca ccgtccgccg actgggccgc cagactcgtc
     4681 gtgctcccac ccgacgggac cgccgagcgg ctcgcggtcg gtgtcgtccg gcgtgccgaa
     4741 ccggccggcc gggaaaccgc gttcaccgtc ggactgggcc ggctggcacg tcggctccgg
     4801 gccggtaccc ggctgcgcct ggagatcgcc ggacaccact tcccggccca cgcccgcaac
     4861 ccccacaccg gggacgaccc gatgacggcc gcacgactgc tcgcctcccg gcgtgaggtc
     4921 cacccggaca gcgtggtgct gaacctgccc gtccttccgt cccgtcccgc ccccaccgac
     4981 cccgccgagg agatcctgcg atgacggccc tgccgctgga agcgctcgtc gaccccgtct
     5041 gcggaatcgt ccgcaaggtc aagcccgtcg agcaccccgc gggcgccccg ccccgctaca
     5101 ccgccctcac ggccgaggtg tccgacgccc gcagacacgg cctgtggccc gccgaccggg
     5161 tctcgctcgg caccaccttc ggcgaccccg agcaggcccg cgtcgccgcg atagccgagg
     5221 ggatggagcg ctactgcggc aaccgtgtgc cgccgcccgg ccacccggac gcacccctgc
     5281 gcgccaccgc cgccgaactg acggacaagg gcctgcgcct gtacggtccg gccgatctgc
     5341 ccgcctacgc accctggcag tacgcccgcg cgaagttccc ctaccgcccg ctcaccgccg
     5401 acaccccggc actgtggaca cggggaaccg aacgactgcc cgacgggacc cccgccgagg
     5461 tctgggcacc cgtctcgctc acccacctca actggcgcca aggcggcctg cgcgagctgc
     5521 cccgcaccca ccacctcaac tacgccggga tcgccaccgg acagggcctc gacgacgctg
     5581 tcgagcgcgg cctgctggag atcgtcgaac gcgacgccct ggaactgtgg tggcacctgg
     5641 acggcccggc ccggggcatc gacccggcga gtgtgcccgg cctctccgac gacctcgccg
     5701 gctccgcgct cgacgtccac atcgtcgaga tgcactcgga gttcgccccc tgcatggcgg
     5761 cgctcgtcca cgacccgcgc ctcggcctgt acgccgccgg gttcgcctgc aagtacgatc
     5821 cggccgaggc tgcccgcaag gccgtcctgg aagccgtcca cacctgggtc ttcacccagg
     5881 gcctgaccgg ccccgacggc tgggtgttcc aggccgtcga ggccgggctg ctcgcccgcg
     5941 ggctgtacct ggaccaccgg gccgacggcc gctacctcga cgtgtgcggc gagcagttcg
     6001 agcacgtgcg cgacctgggc gcacacgtgc aggtgtggct ggacgagcgg atggcggtcg
     6061 aggcccggag gttcaccgag cccgcgctcg ggacggtatc cgtcgcagag gtcgagcccg
     6121 gcagccggga gcggctggag aacacgctgc gcgagggcgg tcaccgtgtc atgaccttcg
     6181 acctcaccac cgaggacgtg gcggagaccg cactgcgcgt cgcccgggtc ctcgtctccg
     6241 gactgcttcc caacgcgccc gccgccttcg gctacttcgg ctgcccgcgc cttgccgagg
     6301 ccgccgtcgc gcgcggatgg cgcgcgactc cgccgagcgc gccggaggat tacacgctgg
     6361 cgcctccgcc acacatgtga gggccgctgt ggcagaccat cgcgaggacc cgcagacggg
     6421 cctcgtcgcc gccctcgccc gggcccgctc cccggagcgc cccggccccg acacgtccgc
     6481 gggagcaccc gtccgggaat gggcgggccc gccgctcccg atcccgcagg ccggccccgc
     6541 gcacctcgac ctgcggactc tgctgcatct ttccctggcg gcctcggaca cctccggacg
     6601 cctcaggccc acgccctccg ccggtgcgct gcacccggtg gacaccgaac tggtcgtggg
     6661 caccggctgc tccctgccgc ccggccgcta cggctacgac ccgctgcgcc accgcgtcca
     6721 tcacgccggc ccgcccgtcg acggcacgcc gccgggcgtg accgccgcac tttccgtcac
     6781 cgcacagcgc acggtgtccc actacggcca ccgggcctgg cccctcctgt tgctggacac
     6841 cgggcacgcc gccgcggcgt tgctcctggc ggcccgcgtc ctgggcgcgg gcgaatccga
     6901 cgtgcggatg gacggacgca ccgagaaccc cctggccgtc gtacgtatcc gaccgcccta
     6961 cgggcggagc ccgaggcact ctgcgaggac tgaggacctt cccaccccgg ccgacctgct
     7021 ggcgcgccgc agcaccccgc caccgttggt gggccttccg ccccgcgacg cactgcgggc
     7081 agtcctgtcc accgccgaac gagccggggg cggtgacctg ttgcgctggt gcgccgcggt
     7141 cggcccgcct cggcccggcc tcatgcaact gggtcccgag ggcacgaccc tgcggcgcta
     7201 cgcgtccggc gaggcccgtc cgacgctcgc ggcctgggcg gcgggccagg cgtggctcgc
     7261 ggacgccggc gccgtactgc tcgcccacgg ctgccccgcg gacgccgacg ccacgcacat
     7321 ccgccgcgcc catctgcgag ccggcttcgc cgtccacctg gcccatgtca cagccctgcg
     7381 ccacgggctg gcggcccgac ccgtcggctc ctggcagcag gctgatctcg gcgccgctct
     7441 cggcgccgcg ccgggccgtg actgggtcgt gcacgccctg gccctcggca cccaccacgc
     7501 cgacgaggag agttccacat gatcgtccac cccgagctgc gtcgcgccgc acggcacgcg
     7561 cggcggcccc tgctcgcggc caccctcttg cagggagccg tcaccctcac gtacctggca
     7621 caggccgtgc tgctggccct cgcgctcgcc ggcctggccc gcggtgacac gggccgactc
     7681 ccgtggctgc tggccggcgt gctcgccgtc gtcgccgccc gcgccgagct cgccctgtgg
     7741 cagcggcgca ccgccacgcg cgccggggcc cgggtcaggg tggcactgcg cgacgaactc
     7801 ctggcccacc tgggccggct ggggcccgca tacctgacca cagcgcgggc gggggccgtc
     7861 cgcaccaccc tggtcgacgg ggtggagggc gtcgacgcct acgtctcccg ctacctgccc
     7921 cagctcctgg tcaccctcgc ggtaccaccc ctggtcctcg ccgtcctcgt cgtggtcgaa
     7981 cccagggccc tcctcgggct cgtccccgcc ctgctcctgg ccctgctggg gccgcgcgcc
     8041 tgggaccggc tcctcgccgc ccgcgggaag gaacactggg acacctacga agggctgggc
     8101 gccgactacc tggaggctct ccagggcatg cccgccctgc gcgccgcggg cgcggtcggc
     8161 cgcacccggg cgcgcctgga ggagcggtcc gcggccctgc accgggcgac cgtcgccaag
     8221 ttgcgggtgt ccctggtcga caccggcatc accgacctcg ccatccaggg cggcaccgcc
     8281 gccgcagcgc tcctggcatg ctggtcggcc gtcaccggat ccaccaccgc ggccggcacc
     8341 tatctggtgc tgctgctggc ctccgagtgc ttccgtccgg tgcgcgacca cgcccgggag
     8401 tggcacgccg gatacctggg ggtgtcggcc gccgacgggc tggcgtcctt gcgcacgtcc
     8461 gtgccggcgg tccccgacac cgcgacggcg cccgcgcact ggcacacccc gcccgaactc
     8521 agttttcaca gcgtccggtt cacctacgac ggtgctgacg cacccgctct ggacggggtc
     8581 tccttcaccg cccgagcggg acggaccacc gcgatcgtcg ggccgtccgg cgcgggcaag
     8641 tcgaccctcc tggccctgct tctgcgccac cacgacccgc aggaagggcg gatcaccctc
     8701 gacggccgcc cgacgaccga gtacgcgctc gactcgctgc ggcagggcat cgccgtcgtc
     8761 tcccaggaga cgtacctgtt ccacgccacc atcgccgaca acctgcgcct ggcccgtccg
     8821 gacgccaccg acgacgagct cacccgggcg gcgcgcgccg ccgggatcca cgacgagatc
     8881 gccgccctcc cggacggcta cgccaccctc ctcggcgaac ggggcgccac gctctccggc
     8941 gggcagcgac agcggctcgc cctcgcgcgg gccctgctgt ccgacgcccc ggtcctcgtt
     9001 ctcgacgagg ccaccagttc ggtcgacgaa cgacgcgagg cggacatcgt ccgagagctg
     9061 ctgaacgagg tcggtgggcg gaccgtcctg gtggttgccc accggctcgc ggccgtccgg
     9121 tccgccgacc ggatcgtcgt cctcgacgcg ggacgggtgg acgccgtcgg cgaccacacc
     9181 ggcctggtcg acgccggcgg cgtctacacc cggctcgtcg aggccggccg cgcccatcga
     9241 ggggaactcg ccgcatgagc accgtcaccg gtcccaccgc cgccggagcc ggcctcccgg
     9301 cgcggggcgc gctgcgcgcc ctgctcccgg ccctcgcggc acaccgtgcc atgacggccc
     9361 gcacctgtgc cgccgcgctc ctcgagcagg gttctctcgt cgcgctggtg accctggccg
     9421 cgcacaccgt gggcatcgcc gtcatcgaag ggcgcgcacc cacggccggc accgtcaccg
     9481 cgctcgtcgc cctcgttctc gtacgcgccc tgatgacctg gcgggggatg gacctctccc
     9541 acgatctcgc ctaccgggta ctggcggcac tgcgggtcca cgtcttcgac ggactggccc
     9601 gcagcgcgcc ggcccgggtc gcgggccggc gcagcggcga tctcgccgcc accgccatgg
     9661 ccgacgtgga ggcgctggag ttcttctacg cccacaccac cgcccagctg ctcgcggcgg
     9721 gaacggtcct caccggcggc tcggtacttc tggccctggt ggagccgtgg ctgctggtgg
     9781 ccgtgctgcc ggtcgccgcc ctgctcgccg cggcgccctt cgccgacgcg ggcgcgcgca
     9841 cggtgcgcgg cggccgcacc cgcacggctg tggccgcgct gtcggccgac acggtggaga
     9901 ccgtcgacgg gctgcgcgaa ctgcttgcct tcggcgctct ggccgaacgc cgccgcacgt
     9961 tggcggaacg ggggcggcag gtcggcgacg cccagcgcgc cgaggccacc tgggaggccg
    10021 tcgccgctgc cgtccgcgac ctcctcatcg tggtcgcggt cctcggcgtg gtcgccgcgg
    10081 cggcacagtc cgtgacctcc ggacggctgc acggtgcctg ggtcccggcc gcgatggcgc
    10141 tggccctgtc cgtcctcggc ccggtcgcgg agtccgcccg cgccctgagc caggccgtgg
    10201 ccctgcgcgc cgccgccgcc cgcgtcgacg cggccgtcaa ggcccccgca ctggcaccac
    10261 cgcccaccgc accacgcccc ctgcctccgg gaccactggg cgtccggttg caccgagtgc
    10321 ggttcgacta cgggggcacc cccgtgctgg acggcgtcga cctcgccgtc cgtcccggcc
    10381 ggacactcgc cctcgtcggc gcctcgggcg cgggcaagtc cacctgcgcc cacctgctgg
    10441 cacgcttctg ggacccgtcc gccggtgagg tccagttgct gcccgccgac ggcgatcccg
    10501 tggacgtgag gcaggtggac gacgcggaac tgcggcgggc ggtggtcctg gtgggacagg
    10561 acacccccct cttccacggc accctcgccg agaacctgcg gctcgccgcg cccgaggccg
    10621 acgaccgggc gctggccgag gcggcgcggc tgtgcggcgt cgaccggatc gcccccctgg
    10681 acaccctcgt cggcgagcgg ggagccacgc tctccggcgg tcagcgggcc cgcatcgccc
    10741 tggcccgcgc gctgctggcc ggcccgcgcg tcctcgtgct cgacgagacc accgcgcacc
    10801 tggacaacac cggcgacgcc gaactcgcca ccgcgctgcg gcaggagagc cgcacgacga
    10861 tcctcatcgc ccaccgcccg gccaccatcc gccgtgccga ccgcatcgcg gtcctggagg
    10921 gcggcctcat cacggaagag ggaacctggg aggacctcac aagcagcccc gacagcgcgc
    10981 tcaaccgcat cctcagtgac accgccctca ctgggcacgg cagttcgccc cgggaaccgg
    11041 accagtgacg cttaggtacg ggtgtacctg gaaacgacta ctcccgaacg atcgtcgcgc
    11101 cggcggtcgg ccggcaacgg gaggggtctc acgtggacgc gcccttgtac ctgaggccca
    11161 gggtggaacc cccgctcgcc gcactgctgg cggacgggcc cttcctgcac tcgctcgccg
    11221 acgcgctggg gtcgccgctc aatgtgctgc tccccgaggc cgtggccgcg aacgccgccc
    11281 gcttccgtgc cgtctaccgc cgccaccgtc tcaccgggcg ggtctacttc gcgcacaagg
    11341 cgaaccggtc cagcgccctg ctgcggcgac tggcggcgga
//
