LOCUS       NZ_BMTZ01000005        10216 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces variabilis strain JCM 4422 sequence05, whole genome
            shotgun sequence.
ACCESSION   NZ_BMTZ01000005
VERSION     NZ_BMTZ01000005.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces variabilis
  ORGANISM  Streptomyces variabilis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces griseoincarnatus
            group.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 429038)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 156X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649935.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:37:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,743
            CDSs (total)                      :: 6,668
            Genes (coding)                    :: 6,496
            CDSs (with protein)               :: 6,496
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 61 of 172
            Pseudo Genes (incomplete)         :: 147 of 172
            Pseudo Genes (internal stop)      :: 11 of 172
            Pseudo Genes (multiple problems)  :: 42 of 172
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:23:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 339821
            Orig. end                         :: 350037
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMTZ01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10216
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="2"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..10216
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10216
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:5216](+)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5216
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    422..431
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            884..3016
                     /locus_tag="IE148_RS12030"
                     /old_locus_tag="GCM10010287_24240"
     CDS             884..3016
                     /GO_function="GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1054: hypothetical
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020700220.1"
                     /locus_tag="IE148_RS12030"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_24240"
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="WP_373302207.1"
                     /transl_table=11
                     /translation="MSEGQASARSHAPVGRPLLSLTLAALMDEVHAHSGAVYLLSPDQP
                     VLEMAVMAGLPRAFAAPWERVGLSAPIPVSDAVRERRLVWVGGGEEMARHYPRIAMVLP
                     YPFSLAALPVATPAKTYGAVFVTWPGSHPPELSDREREHLTAACDRLAVRLERAAEHSL
                     SLAHEPDLLAAPVVGGVAGSLGTVEAARMVSRLPYGLCSLDLHGRVAFANAAASDLLGV
                     APDRLPGAHLWAAVPWLNDPVYEDRYRAALFSQDVTSFVALRPPDTWLSFRLYPSTTGI
                     SMRISRARAVTEMNRAAPPQAGAGTDRLVTITQVLGLAGALTEAVGVQDVVQLVADEIV
                     PAVGGQGLVVLGSRAGRLRVLGHRGYADPLVVERFDGLPLSEPTPGGQVLRTGVPAFFE
                     SRGDLERLYPALQTHPSEFSAWAYLPLIASGRPVGTCVLSYGAPHRFATEERAVLTSLA
                     GLIAQALERAMLYDVKHRLAHGLQEALLPSTLPALEGIEAAARYLPATRGMEIGGDFYD
                     LVPTDGVVAAVIGDVQGHNVTAAGLMGQVRTAVRAYTAVGQAPDEVMRSTNRLLLDLGA
                     ELFASCLYLRLDAVRGRAVMARAGHPPPLLRRPDGRVRVLDLAGGPLLGIDGTAAYPST
                     EVALPPGSLLVLYTDGLVESPGVDLEDALAELCGRLTELGDLRLDELAEALVRHGPHSA
                     DRLDDTAVLLLRSLPR"
     gene            complement(3094..4428)
                     /locus_tag="IE148_RS12035"
                     /old_locus_tag="GCM10010287_24250"
     CDS             complement(3094..4428)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994899.1"
                     /locus_tag="IE148_RS12035"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_24250"
                     /product="S1 family peptidase"
                     /protein_id="WP_189364515.1"
                     /transl_table=11
                     /translation="MSHKRIPKRKAAMAVGGVVALGAAAILLPNANASQDGASDGVAAA
                     PKTLKAGDASDLATRLAGTLGDAYAGAWYDGEDKQLVVNVVRGENTMALKVRSAGAEVR
                     WVDNSMTELEAGAGTLKSEATIPGTSWAVDPRTNKILVTADSTVTGDKWDQLESTVENL
                     GSGMATLKKSAGTFRTFASGGDAIFAGGARCSLGFNVTAGDGSPAFLTAGHCAAAGDQW
                     SDQQGGEPIATVDQALFPGAGDFALLTYDDPATEAPSEVNVGGQTVPVVQAAEATVGQQ
                     VFRMGSTTGLADGQVLGLNATVNYPEGTVTGLIQTDVCAEPGDSGGSLFTQDGLAIGLT
                     SGGSGDCTVGGETFFQPVTTALEAVGATLGDGGAGAGGQAGGQEQGAGQDAGDGEAGAD
                     DQAGAGDQADGQGVGTGQDGAGGQDGTGTGSGRHSGQDSGLSRTH"
     misc_feature    complement(4972..4985)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.44"
                     /tool="antismash"
     gene            5001..5216
                     /locus_tag="IE148_RS12040"
                     /old_locus_tag="GCM10010287_24270"
     CDS             5001..5216
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: strepbact"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128984.1"
                     /locus_tag="IE148_RS12040"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_24270"
                     /product="hypothetical protein"
                     /protein_id="WP_019527181.1"
                     /sec_met_domain="strepbact (E-value: 1.5e-36, bitscore:
                     119.4, seeds: 15, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNSTPQVETVEIADAELDAVSGGLSVNAVNTLTGALDGIAPVSGL
                     VDTAVGTVEGVTGLNTAPVTNLVAGL"
     gene            5328..6134
                     /locus_tag="IE148_RS12045"
                     /old_locus_tag="GCM10010287_24280"
     CDS             5328..6134
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386622.1"
                     /locus_tag="IE148_RS12045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_24280"
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="WP_086699282.1"
                     /transl_table=11
                     /translation="MQFRQQALARLQSPEDLDLPVRFARPQGWLVLSVTVIAMAAASVW
                     ALTGSVTSTVQAPAILTYGQGSYILQSPVAGQVTAVLAREGERLPAGSPVLRVRTPEGD
                     EVVRTLQAGRITALAAGAGQIIATGADVAAVEKVSRAKAPLYATVYVPAQKAAGIPAHA
                     SVDLTVQSVPSQEYGVLRGEVTEVDRSAQSPRQIAAFLGDTQLGEEFTRGGRPVAVTVK
                     LKRSDSTESGYAWSSADGPPFELTSMTLATGSIRLSDRRPVDWLLP"
     gene            6131..8353
                     /locus_tag="IE148_RS12050"
                     /old_locus_tag="GCM10010287_24290"
     CDS             6131..8353
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Peptidase_C39"
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003978106.1"
                     /locus_tag="IE148_RS12050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_24290"
                     /product="NHLP family bacteriocin export ABC transporter
                     peptidase/permease/ATPase subunit"
                     /protein_id="WP_086699283.1"
                     /sec_met_domain="Peptidase_C39 (E-value: 6e-40, bitscore:
                     130.8, seeds: 36, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTAQETRGRRRAAPPRRKVPTSRVRTVRTPTVLQMEAVECGAAS
                     LAMVLGHYGRHVPLEELRIACGVSRDGSRASNLLKAARSYGLTAKGMQMDLAALAEVTA
                     PAILFWEFNHYVVYDGMGRRLGRRGVYVNDPAKGRRFVPLEEFDGSFTGVVLVMEPGED
                     FTRGGRRPGVLGAMPARLRGTAGTMPAVVLTSLLLVAVGAALPGLSRTYIDEFLIGQQT
                     SMLDVLFASMGACVLLTLLLTWLQQANLLRGRLISSTLSSARFLRHLLRLPVTFFSQRS
                     PADLVQRLQSNDAVAETLARDLSAAGVDAVVVVLYAVLLYTYDPQLTFVGIGVALLNIL
                     AMRIVIRLRATRTAKLRADTARLTNTAYTGLQLIETLKATGGEDGYFRKWAGQHATTLE
                     EQQRLGVPSAWLGIVAPTLATLNSALILWIGGMRAVEGHISVGLLVAFQALVARFTAPL
                     TRLNGVAGRIQDFAADVARLKDVENFRADPLYGRPGAGESTRRLHGHVELQNLTFGYNP
                     LDKPLLTGFDLTVGPGQQVALVGGSGSGKSTVSRLISGLYTPWEGVIRIDGQRIEDIPR
                     GALAASVSFVDQDVFLFEGTVRDNVALWDPSIPDEAVVEALKDAALYDVIVRRPGGIHS
                     PVEQDGRNFSGGQRQRLEIARALVRRPSILVLDEVTSALDAETELVVMDNLRRRGCACV
                     VIAHRLSTVRDSDEIVVLDQGTVVERGRHEELVARGGAYAALVRER"
     misc_feature    8643..8656
                     /note="TFBS match to BldD, Development and antibiotic
                     global regulator, confidence: weak, score: 19.03"
                     /tool="antismash"
ORIGIN
        1 gcacccggag ccggccggtg tccatggcga ggctgaagcc gcccggcggc aggtccgcgc
       61 cctcggcggt gacgggcggc gcggggaagg cgacggcgtc cgcgacgagg gcgaggcacg
      121 cccggtcgtc ggcgtcgaac ccgccgggcc gctcgctcac cgcgagcaga cagccgccgc
      181 cgtcgtggcg cacgggcagg gcggcgaggt ggaactcgcg gcggggcgtg cggcgcgact
      241 gggactgggc ggccaggtcc ctcgggccga gatacaccgt gtgtccccgg tgcgcctcgg
      301 cgaccgggga gcggccggcg gcggagtagc agtcgcgcac cccgcacagc gtgcgcggca
      361 ccccggcggt ctccgccagg cacagcagct cgccgtcctc gccgaggctg tagaagccgg
      421 cgaaggcggc cccggcgaag acgagggcct gttccaggat gcggcgcagc cggtcggtcg
      481 cgtgggggtc ggccgcgagc gcgctcagtg cgccttcggc gcgcggcgtt ctcgtgctgc
      541 gtcccgcagc gccctcaccg accacgttgg ccattacagc gctaatagga cacaagcgca
      601 gcccctgtgc gcgaacggca cgcggcaggg ccgcgccggt tctcacgcgg ggtacgcgcc
      661 cggctcgcgg tgcgcgcgcc cgcgacggtg cgtggcccgg gccacccgga cgtccgcggg
      721 gttccgcccc ggaccccgcg cctcgaacgc cggaggggct gagctcgcct taccgcgacg
      781 caacggggta tccgcacaga tgcgcgcccg gggcgtcccg ctcgccgtgc gggacgccct
      841 cccgcagggg agattgggtg cggggcgcga ggaggtggtc ggcgtgtccg aaggacaggc
      901 gtccgcgcgg tcgcacgccc ccgtgggccg tccgctgctg tcgctgacgc tggccgcgct
      961 gatggacgag gtgcacgccc actccggcgc cgtgtatctc ctctcccccg accagcccgt
     1021 gctggagatg gccgtgatgg cggggctgcc ccgcgccttc gccgcaccgt gggagcgggt
     1081 cgggctgagc gcgccgatcc ccgtctccga cgcggtgcgc gaacggcggc tggtgtgggt
     1141 gggcggcggc gaggagatgg cccgtcacta cccccggatc gccatggtcc tcccctaccc
     1201 cttctccctg gccgcgctcc cggtggcgac gccggcgaag acgtacggcg cggtcttcgt
     1261 cacctggccg ggctcgcacc ccccggagct gtcggaccgg gagcgtgagc atctcaccgc
     1321 cgcctgcgac cggctcgcgg ttcggcttga gcgggccgcc gagcacagtc tgagcctcgc
     1381 ccacgagccg gacctgctgg ccgcgccggt ggtgggcggg gtggccggct cgctgggcac
     1441 ggtggaggcg gcccggatgg tgtcccggct gccgtacggg ctgtgctcgc tcgatctgca
     1501 cgggcgggtc gcgttcgcca acgccgccgc gtccgacctg ctcggcgtgg cgccggaccg
     1561 gctgccgggc gcgcatctgt gggcggcggt gccgtggctg aacgacccgg tgtacgagga
     1621 ccggtaccgg gcggcgctgt tcagccagga cgtcacgtcg ttcgtggcgc tgcggccgcc
     1681 cgacacctgg ctgtccttcc ggctgtaccc gagcacgacc ggtatcagca tgcggatcag
     1741 ccgggcccgc gcggtgacgg agatgaaccg cgcggcgccg ccgcaggcgg gtgcgggcac
     1801 ggaccggctg gtgaccatca cccaggtgct cggtctggcc ggggcgctga ccgaggcggt
     1861 cggggtgcag gacgtggtgc agctggtcgc ggacgagatc gtcccggcgg tgggcggcca
     1921 ggggctggtg gtcctcggct cccgcgcggg ccggctgcgc gtactcgggc accgcgggta
     1981 cgcggacccg ctggtggtgg agcggttcga cgggctgccg ctgtccgagc cgacgccggg
     2041 cggtcaggtg ctgcgcaccg gggtgcccgc cttcttcgag tcgcgggggg acctggagcg
     2101 gctgtacccg gcgctgcaga cgcatccgtc ggagttctcg gcgtgggcgt acctgccgct
     2161 gatcgcctcg ggccgcccgg tggggacgtg tgtgctgtcg tacggggcgc cgcaccggtt
     2221 cgcgacggag gagcgggcgg tgctgacgag tctggcgggt ctgatcgccc aggcgctgga
     2281 gcgggcgatg ctctacgacg tgaagcaccg gctggcgcac gggcttcagg aggcgctgct
     2341 gccgagcacc ctgccggcgc tggaggggat cgaggccgcc gcccggtatc tgcccgccac
     2401 ccggggcatg gagatcggcg gcgacttcta cgacctggtg ccgaccgacg gggtggtggc
     2461 ggcggtgatc ggggacgtgc aggggcacaa cgtgacggcg gccgggctga tgggccaggt
     2521 ccgtacggcg gtgcgggcgt acacggcggt gggacaggcg ccggacgagg tgatgcgcag
     2581 caccaaccgg ctgctgctgg acctgggcgc ggagctgttc gcgagctgtc tgtatctgcg
     2641 gctcgacgcg gtgcgcgggc gggccgtgat ggcgcgggcg gggcatccgc cgccgctgct
     2701 gcgccgtccc gacgggcggg tgcgggtcct cgacctggct ggcgggccgc tgctgggcat
     2761 cgacggcacg gcggcctatc cctcgacgga ggtggcgctg ccgcccggtt cgctgctcgt
     2821 cctgtacacg gacggtctgg tggagtcgcc gggcgtcgac ctggaggacg ccctcgccga
     2881 gctgtgcggc cggctcaccg agctcggcga cctgcggctg gacgagctgg cggaggcgct
     2941 ggtgcggcac ggcccgcact cggcggaccg gctggacgac acggcggtgc tgctgctgag
     3001 gtccctgccg cgctgaccgg ccccgcggcg cggagacgcc ccggcccgca cacggccggg
     3061 gcgcctcagg cacacggttc cgacgcgcgc gggtcagtgc gtccgggaca gcccggagtc
     3121 ctgaccggag tgacggccgg agcccgtacc ggtcccgtcc tgtccgcccg cgccgtcctg
     3181 cccggtgccg acgccctggc cgtccgcctg gtcaccggcg cccgcctggt cgtccgcgcc
     3241 cgcctcaccg tcgccggcgt cctggcccgc gccctgctcc tggccgcccg cctggccgcc
     3301 ggcgcccgca ccgccgtcgc cgagggtcgc gccgacggcc tcgagggcgg tggtgacggg
     3361 ctggaagaac gtctcgccgc ccaccgtgca gtcaccgctg ccaccggagg tcagaccgat
     3421 ggccagaccg tcctgggtga agagggagcc gccgctgtcg ccgggctccg cgcacacgtc
     3481 ggtctggatg agcccggtga ccgtgccctc ggggtagttc acggtggcgt tcaggccgag
     3541 cacctggccg tcggcgaggc cggtggtact gcccatgcgg aacacctgct gtccgacggt
     3601 ggcctcggcg gcctggacga ccggcaccgt ctggccgccg acgttcacct cgctcggggc
     3661 ctcggtggcc gggtcgtcgt aggtgaggag cgcgaagtcg cccgcgccgg ggaacagcgc
     3721 ctggtcgacc gtggcgatcg gttcgccgcc ctgctggtcg gaccactggt cgccggcggc
     3781 ggcgcagtgt ccggcggtga ggaacgccgg gctgccgtcg cccgctgtga cgttgaagcc
     3841 gagcgaacag cgcgccccgc ccgcgaagat ggcgtcgccg ccggacgcga aggtgcggaa
     3901 ggtgcccgcg gacttcttga gggtggccat gccggagccg aggttctcga cggtggactc
     3961 cagctggtcc cacttgtcgc cggtgaccgt gctgtccgcg gtgacgagga tcttgttggt
     4021 gcgcgggtcg accgcccagg aggtgcccgg gatcgtcgcc tccgacttca acgtccctgc
     4081 tccggcctcg agttcggtca tgctgttgtc gacccaccgg acctccgcgc ccgcgctgcg
     4141 gaccttgagg gccatggtgt tctcgccgcg caccacgttg acgacgagct gcttgtcctc
     4201 gccgtcgtac caggcgcccg cgtaggcgtc gccgagcgtt ccggcgagcc gggtggcgag
     4261 gtccgaggcg tcgcccgcct tcagtgtctt cggcgcggcc gcgacgccgt ccgacgcgcc
     4321 gtcctgggac gcgttggcgt tggggagcag gatcgcggcg gcgccgaggg ccaccacacc
     4381 tcccaccgcc atcgcggcct tgcgcttcgg gattcgcttg tgactcaaac ttctcgacct
     4441 cctgggggga ccggagtcgt gccgccgtcc gacggctgtt cggggcgcct ggacggtcgt
     4501 tggtacgcaa gggagttgag gggtgttcaa cgcggagcgg atcctcaccg tccgccaccg
     4561 gccggggacg ctcggtgtcc gacccccggt cgaatcccgg cttcggcgaa tctgcacagc
     4621 gaatgcccaa cccggtcacc tgacccgccg tccgttcaca ggccgcgcat ccgggacacg
     4681 cctttgcggc cggtgtgtcg ctttccggca ccgggggcgc aagcggccgg atgcgtcgat
     4741 gccgtggccc atgtgaagaa gtcgccgccg agccgtgccc tcgtctgcac ggttgggtgc
     4801 ctcctgggcg caagttgcct ggtggggcgt gtgagggatt ggaagcggcc ggttgccgcg
     4861 tgctttgatc catcgcaccg caaggccgtt ccgggctccc ggcgacgggc gctcggagcg
     4921 cccgcggtcg cggccttcat ctgtccccag aggggggccg ctcccccctt gtgaaagtcc
     4981 atacgaaggg aagttccatc atgaactcca ccccccaggt cgagaccgtc gagatcgccg
     5041 acgccgagct cgacgccgtc tccggcggtc tgtccgtcaa cgccgtgaac accctcaccg
     5101 gcgccctgga cggcatcgcc ccggtttccg gcctggtcga cacggccgtc ggcaccgtcg
     5161 agggtgtcac cggcctgaac acggccccgg tcaccaacct ggtcgccggt ctctgatcgc
     5221 gccctcgttc cgccgagtcc cggagccgta ccccggctcc gggactctgt ggtgcgtccg
     5281 cagtccttcc gtcacgtcgc acagttcacc caggtgaggg aagacccgtg caattccgcc
     5341 aacaggccct cgccaggctc cagtcgccgg aggatctcga cctcccggtg cgtttcgccc
     5401 gcccgcaggg ctggctggtg ctgtccgtca ccgtgatcgc gatggccgcc gcgtccgtgt
     5461 gggcgctgac cggctcggtc acctccacgg tccaggcgcc cgccatcctc acgtacgggc
     5521 aggggagtta catcctgcag agccccgtcg ccggtcaggt caccgccgtg ctcgcgcgcg
     5581 agggtgagcg gctgccggcc ggctcccccg tgctcagggt ccgtaccccg gagggggacg
     5641 aggtggtgcg tacccttcag gcgggccgga tcaccgcgct cgccgccggc gccgggcaga
     5701 tcatcgcgac cggcgcggac gtcgccgcgg tagagaaggt gagccgcgcg aaggccccgc
     5761 tgtacgcgac ggtgtacgtg cccgcgcaga aggccgccgg cattcccgcg cacgcctccg
     5821 tcgacctgac ggtgcagtcg gtgccgagcc aggagtacgg cgtgctgcgc ggcgaggtga
     5881 cggaggtgga ccgctccgcg cagtccccac gccagatcgc cgcgttcctc ggcgacaccc
     5941 agctcggcga ggagttcacc cggggcggca ggcccgtggc ggtcaccgtg aagctgaagc
     6001 gctccgacag cacggagagc ggctacgcgt ggtcctcggc cgacggcccg ccgttcgagc
     6061 tgacgtcgat gaccctggcg accggttcca tccggctgtc cgaccggcgt cccgtggatt
     6121 ggctgctgcc gtgagcaccg cacaggagac ccgcggcaga cgccgcgccg caccgccgcg
     6181 ccgcaaggtg cccacgtcca gggtcaggac ggtccgtacg cccaccgtgc tccagatgga
     6241 ggccgtggag tgcggcgccg cctcgctcgc gatggtgctg ggccactacg gccgtcacgt
     6301 cccgctggag gagctgcgca tcgcgtgcgg cgtctcgcgg gacggctcgc gcgccagcaa
     6361 cctgctgaag gcggcccgca gttacgggct gaccgccaag ggcatgcaga tggacctggc
     6421 cgccctcgcc gaggtgacgg cgccggccat cctgttctgg gagttcaacc actacgtcgt
     6481 ctacgacggc atgggccgcc gcctcggccg tcgcggggtc tacgtcaacg accccgccaa
     6541 aggccgccgg ttcgtgccgc tggaggagtt cgacggcagc ttcaccggcg tggtgctggt
     6601 gatggaaccc ggcgaggact tcacccgggg cggccgcagg ccgggcgtgc tcggcgcgat
     6661 gccggcgcgg ctgcgcggta ccgcgggcac catgcccgcc gtggtgctga ccagtctgct
     6721 gctggtggcg gtgggcgcgg cgctgcccgg cctcagccgt acctacatcg acgagttcct
     6781 gatcgggcag cagacgtcga tgctggacgt gctgttcgcg tcgatgggcg cctgcgtcct
     6841 gctgacgctg ctgctgacct ggctccagca ggcgaacctg ctgcgcggcc ggctgatctc
     6901 gtccacgctg tccagcgcgc gtttcctgcg tcatctgctg cggctgccgg tgacgttctt
     6961 ctcccagcgc agccccgccg acctggtgca acgcctccag tccaacgacg cggtggccga
     7021 gacgctggcc cgtgatctgt cggcggctgg cgtcgacgcg gtcgtggtgg tgctgtacgc
     7081 ggtgctgctg tacacgtacg acccgcagct cacgttcgtg ggcatcgggg tggcgctgct
     7141 gaacatcctg gcgatgcgga tcgtgatccg gctgcgcgcc acccgcacgg cgaagctgcg
     7201 cgcggacacg gcgcggctca ccaacaccgc gtacacgggg ctccagctga tcgagacgct
     7261 gaaggccacc ggcggcgagg acggctactt ccgcaagtgg gccgggcagc acgccaccac
     7321 gctggaggag cagcagcggc tcggggtgcc gagcgcgtgg ctgggcatcg tcgcgccgac
     7381 gctggccacg ctgaacagcg cgctgatcct gtggatcggc ggcatgcggg cggtcgaggg
     7441 gcacatctcg gtcggtctgc tggtcgcctt ccaggcgctg gtcgcccggt tcacggcgcc
     7501 gctgacccgg ctgaacgggg tggcgggccg catccaggac ttcgccgccg acgtggcccg
     7561 gctgaaggac gtcgagaact tccgggcgga cccgctgtac gggcgtccgg gcgccggtga
     7621 gtcgacccgg cggctgcacg ggcatgtgga gctgcagaac ctgaccttcg gctacaaccc
     7681 gctggacaag ccgctgctga ccggcttcga cctgacggtc ggtccgggcc agcaggtggc
     7741 gctggtgggc ggctcgggca gcggcaagtc gaccgtgtcg cggctgatct cggggctgta
     7801 cacgccgtgg gagggcgtga tccggatcga cgggcagcgc atcgaggaca tcccgcgcgg
     7861 ggcgctggcc gcgtccgtct ccttcgtcga ccaggacgtg ttcctgttcg aggggacggt
     7921 gcgggacaac gtggcgctgt gggacccgtc catccccgac gaggcggtgg tcgaggcgct
     7981 gaaggacgcg gcgctgtacg acgtgatcgt gcgccgtccg ggcggcatcc acagcccggt
     8041 cgagcaggac ggccggaact tctccggcgg tcagcgccag cgtctggaga tcgcgcgggc
     8101 gctggtgcgc cggccgagca tcctggtgct cgacgaggtc accagcgcgc tggacgccga
     8161 gacggagctg gtggtgatgg acaacctgcg ccgccgcggc tgcgcgtgcg tggtgatcgc
     8221 gcaccggctg tccacggtcc gcgacagcga cgagatcgtc gtgctggacc agggcacggt
     8281 cgtggagcgc ggacggcacg aggagctggt ggcgcgcggc ggcgcgtacg cggcgctggt
     8341 cagggagcga tgagatgact gccgtgcagg aggggcgggg cggcgacgtc gtcctgggcg
     8401 cgctcggcgc gctgggcgcc ccgatcgact gctccgggtt cgggcggctg gacctggagg
     8461 ggccgcaggt gctgtggctg atcgcgtccg gcgccgtgga cctgttcgcg gtggacgcgg
     8521 agcagcaggg gcactggcac cacctgggcc gtctggaggc gggctcgctg gtgccgggcc
     8581 cggtggcggg cccggcgcac acgctggtgg cgcggccgct gcgggactgc gtggtgcacc
     8641 gcatcgcgct gcgtgagctg taccaggagg cccacaccca gacctggtcc tacgacgagt
     8701 acggcaaccc gcagtacgtg ccgccgacga cgagcccgct ggagtacgcg ctcgcgctgg
     8761 gcgtgggccg cagcctcgcc gtgctgttcc aggcgccgat ggcgacggag cgggcggcgg
     8821 cgcccaccga cgacgacgtg ttctggatgc aggtgccccc gggcagcgtg cagtacggct
     8881 ccgtgtacgg cgcggaggcc gcggccgatc tgctgatgga tccgtcactg tggcagagca
     8941 tggtcgacca gcagtaccgg ctgatgacca cgctggaccg gtggatcgag cagctggagg
     9001 agacgcacga gtcgcgcacc gcgcagggca tcgcggccgg cgaggcggtg cgggcgcacg
     9061 ccgaccggac gctgctcgcg tcgatcggca agcggtcggg gcagcgcacg acggccgcgg
     9121 acgcggacgc cacgtacgcg gcctgcgagc tggtggcgcg ggcggcgggg atcacgctgg
     9181 cctcgccggc ccggtccggc accggcgccg accggctgga tccggtggag caggtggcgc
     9241 tggcctcgcg gctgcgcacc cgggtggtgc gtcttgaggg ccggtggtgg cgcgacgacg
     9301 tggggccgct ggtgggtcac cgcgccctgt cgggggcgcc ggtggcgctg gtgtggcggc
     9361 gcggcggcta tgtggccgtt catccgtcga cgggccggga gaccccggtg gagaaggcga
     9421 acgccgagga gttcgagccg cgcgcggtga tgttctaccg tccgctgccc gagcggcggc
     9481 tgagcccgct gcggctgatg cggttctgca tgggcggcac ccggggcgac ctgacggggc
     9541 tgctgctgag cggtctggtg acggtggcgc tcggggcgct ggtgccgctg gcgacgggcc
     9601 gggtgctcgg cgagtacgtg ccgaaggccc ggacggacct catcgtgcag gtgtgtctgg
     9661 cggtgatcct cagcagtgtg gtcgcggccg cgttcatgct gctgcagaac ctcacgctgc
     9721 tgcggctgga gggccgggtg gaggccacgc tccagccggc ggtgtgggac aggctgctcc
     9781 ggctgccgac ccggttcttc acggagcggt ccacgggtga gctggcgagc gcggcgatgg
     9841 gcatcagcgc catccgccgg atgctggccg gtgtcgggcc cacggtgacc cagtcggtca
     9901 cggtgggcgc ggtgaacctg gcgctgctgc tgtggttcag cgtgccgatg gcgctggccg
     9961 cgatgggcat gctggtggtg atcgcgggcg tgttcctggg tcttgggctg tggcaggtgc
    10021 gctggcagcg acggttggtg gtgcttgcca acaagctgaa caaccaggcg ttccagacgc
    10081 tgcgcgggct cccgaagctg cgcgtggcgg cggcggagaa ctacgcgtac gcggcctggg
    10141 cgtcgcagtt cgcgcgcagc cgtgagctcc agcagaaggc cgggcggatc aggaatctga
    10201 cggcggtgct gggcgc
//
