LOCUS       NZ_BMTZ01000005         8615 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces variabilis strain JCM 4422 sequence05, whole genome
            shotgun sequence.
ACCESSION   NZ_BMTZ01000005
VERSION     NZ_BMTZ01000005.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces variabilis
  ORGANISM  Streptomyces variabilis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces griseoincarnatus
            group.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 429038)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 156X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649935.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:37:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,743
            CDSs (total)                      :: 6,668
            Genes (coding)                    :: 6,496
            CDSs (with protein)               :: 6,496
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 61 of 172
            Pseudo Genes (incomplete)         :: 147 of 172
            Pseudo Genes (internal stop)      :: 11 of 172
            Pseudo Genes (multiple problems)  :: 42 of 172
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:23:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 8615
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMTZ01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..8615
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..8615
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..8615
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[3216:3615](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(3217..3615)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            complement(<1..>1003)
                     /locus_tag="IE148_RS10425"
     CDS             complement(<1..1003)
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: NAD_binding_4 (72-161). E-value:
                     6.4e-07. Score: 20.7. Matches aSDomain:
                     nrpspksdomains_IE148_RS10425_NAD_binding_4.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) RmlD_sub_bind"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010: NAD-dependent epimerase/dehydratase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014050041.1"
                     /locus_tag="IE148_RS10425"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_308430239.1"
                     /sec_met_domain="RmlD_sub_bind (E-value: 1.4e-14, bitscore:
                     48.5, seeds: 37, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSSWPSPVRGTVLVVGGSGFIGSAVVGELAARAAARGGPAVRVLA
                     RRAPLSRDGVVGHVRADLTRPGSLRGALAGVEVVVHAAAYVGRDAARCEAVNHGGTLAL
                     LEEARRAGVGHVVYVSTASVYGVGPHRGADEHALVPRPASPASMSRLRAERAVLDAGGT
                     VLRPHLVYGVGDEWFIPALARLLTEVPAWPAGRASRSSLIAVGDLARGVAGLACAPSAA
                     DRSAVYHAAHPRPVAADRLLSALGRHLGFPVPRGDVSPGEHRGLLARAVPELSDHQYAL
                     LTQDHWYRADRLWSRIGEDPGPGFEARFAEAAHWYAARLAGRGRSRAGRGAGP"
     aSDomain        complement(521..787)
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE148_RS10425_NAD_binding_4.1"
                     /evalue="6.40E-07"
                     /label="IE148_RS10425_NAD_binding_4.1"
                     /locus_tag="IE148_RS10425"
                     /protein_end="161"
                     /protein_start="72"
                     /score="20.7"
                     /tool="antismash"
                     /translation="LAGVEVVVHAAAYVGRDAARCEAVNHGGTLALLEEARRAGVGHVV
                     YVSTASVYGVGPHRGADEHALVPRPASPASMSRLRAERAVLDAG"
     CDS_motif       complement(896..973)
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE148_RS10425_0001"
                     /evalue="1.60E-03"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE148_RS10425"
                     /protein_end="36"
                     /protein_start="10"
                     /score="11.1"
                     /tool="antismash"
                     /translation="TVLVVGGSGFIGSAVVGELAARAAAR"
     assembly_gap    1004..1036
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1875..2225)
                     /locus_tag="IE148_RS10430"
                     /old_locus_tag="GCM10010287_20920"
     CDS             complement(1875..2225)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018549458.1"
                     /locus_tag="IE148_RS10430"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_20920"
                     /product="SAV_915 family protein"
                     /protein_id="WP_189364349.1"
                     /transl_table=11
                     /translation="MADARRDADPTPGGRPSADLLYVPVRPHRRCCVTCFFRTPGGRRT
                     AVAFTSCARLLAALGQDHPWIRLSVTALRALTAPLGCTTITVDPQVAVRPGASRTSRAG
                     PGRSLLPGARAN"
     gene            complement(2254..3150)
                     /gene="thpD"
                     /locus_tag="IE148_RS10435"
                     /old_locus_tag="GCM10010287_20930"
     CDS             complement(2254..3150)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989748.1"
                     /locus_tag="IE148_RS10435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_20930"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_373302209.1"
                     /sec_met_domain="PhyH (E-value: 4.2e-36, bitscore: 119.8,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MHHHRLSTDVYPTRGTAEAMMPRRDPVVWSAPAAPGPLGGAGLAD
                     VDRAGFTTVEALLSPGEVALFRAELDRLVRDPAVGCDERAVVEPASRAIRSVFEVHRIS
                     EVFASLVRDERVVGAARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPR
                     MRAVSVSIALTDNHATNGSLMIMPGSHRTFLGCAGATPRDNYKKSLQMQDAGTPSNEAV
                     TALCEAGGIRLITGKAGSATWFDCNCMHGSGDNITPYPRSNVFIVFNSVENTAGEPFAA
                     PARRPAFVAARDFTPVR"
     gene            complement(3217..3615)
                     /locus_tag="IE148_RS10440"
                     /old_locus_tag="GCM10010287_20940"
     CDS             complement(3217..3615)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993572.1"
                     /locus_tag="IE148_RS10440"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_20940"
                     /product="ectoine synthase"
                     /protein_id="WP_189364351.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.1e-48, bitscore:
                     159.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIIRSLEEIEDTVRHVRAASGTWESKRLVLAREQAGFSLHVTTLY
                     AGTQTTMRYTHHVEAVFCVEGEAELTDEESGEKHWITPGTVYLLDGHERHTMRPKTDVR
                     CVCVFNPPLTGREDHDAEGAYPPPGPGA"
     gene            complement(3745..5040)
                     /gene="ectB"
                     /locus_tag="IE148_RS10445"
                     /old_locus_tag="GCM10010287_20950"
     CDS             complement(3745..5040)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (29-365). E-value: 7.1e-68.
                     Score: 221.0. Matches aSDomain:
                     nrpspksdomains_IE148_RS10445_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015576146.1"
                     /locus_tag="IE148_RS10445"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_20950"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_189364352.1"
                     /sec_met_domain="Aminotran_3 (E-value: 1.5e-74, bitscore:
                     245.7, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTVSRPASHVFERLESEVRSYCRGWPVVFDRALGSRMYGEDGRPF
                     LDFFSGAGALNYGHNNPVLKRALVDYIERDGVTHALDMSTAAKRTFLETFQETVLEPRE
                     LPYKVMFPGPAGTNAVEAALKLARKVKGREAVVFFTNAFHGMSLGSLAVTGNAFKRAGA
                     GVPLAHAMPMPYDGGPCGGEGAFSWLERLLAGEGSGLDTPAAVIVETVQGEGGINVARS
                     EWLRGLAGLCRRRDMLLIVDDIQMGCGRTGAFFSFEQAGIVPDIVTLSKSVSGYGLPMA
                     LTLLRPELDIWRPGEHNGTFRGFNPAFVTATAALEEYWRDGRLQKQTLARGEQMEQGLR
                     AVAASRPAVAPEVRGRGLAWGVEFADPSRAAAVCARAFELGLLLETSGPRDEVVKLFPP
                     LTIEEQELEEGLALFASAVRGTERPARSAGPR"
     aSDomain        complement(3946..4953)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE148_RS10445_Aminotran_3.1"
                     /evalue="7.10E-68"
                     /label="IE148_RS10445_Aminotran_3.1"
                     /locus_tag="IE148_RS10445"
                     /protein_end="365"
                     /protein_start="29"
                     /score="221.0"
                     /tool="antismash"
                     /translation="DRALGSRMYGEDGRPFLDFFSGAGALNYGHNNPVLKRALVDYIER
                     DGVTHALDMSTAAKRTFLETFQETVLEPRELPYKVMFPGPAGTNAVEAALKLARKVKGR
                     EAVVFFTNAFHGMSLGSLAVTGNAFKRAGAGVPLAHAMPMPYDGGPCGGEGAFSWLERL
                     LAGEGSGLDTPAAVIVETVQGEGGINVARSEWLRGLAGLCRRRDMLLIVDDIQMGCGRT
                     GAFFSFEQAGIVPDIVTLSKSVSGYGLPMALTLLRPELDIWRPGEHNGTFRGFNPAFVT
                     ATAALEEYWRDGRLQKQTLARGEQMEQGLRAVAASRPAVAPEVRGRGLAWGVEFA"
     gene            complement(5104..5580)
                     /gene="ectA"
                     /locus_tag="IE148_RS10450"
                     /old_locus_tag="GCM10010287_20960"
     CDS             complement(5104..5580)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014157665.1"
                     /locus_tag="IE148_RS10450"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_20960"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_086698791.1"
                     /transl_table=11
                     /translation="MAVPTVKDGAAVWRIARDSGVLDVNSPYCYLLWFRDFARTSVVAS
                     QSGDVPVGFVTGFRRPEQPDTLVVWQVAVDRGQRGRGTAGAMLDHLTARLAAGGTLRHL
                     ETTISAGNAASEGLFRSFARRHAAPLERDLLFPAEFFPDAHDAEFLYRIGPLLR"
     misc_feature    5828..5841
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 15.79"
                     /tool="antismash"
     misc_feature    5829..5842
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 17.83"
                     /tool="antismash"
     misc_feature    6007..6020
                     /note="TFBS match to ANR, Anaerobic transcriptional
                     regulator, confidence: weak, score: 19.7"
                     /tool="antismash"
     misc_feature    6369..6384
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.63"
                     /tool="antismash"
     misc_feature    6491..6504
                     /note="TFBS match to CatR, H2O2-responsive repressor,
                     confidence: medium, score: 18.76"
                     /tool="antismash"
     gene            complement(6667..7011)
                     /locus_tag="IE148_RS10455"
     CDS             complement(6667..7011)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF021524.5"
                     /locus_tag="IE148_RS10455"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF2254 family protein"
                     /protein_id="WP_189364353.1"
                     /transl_table=11
                     /translation="MRCEPLPRSAVLLEVHGQPRTPPEPRRLAGTVALGAERTVERDPA
                     FAPRVLAGVAAQALSSAVNDPTIAVQVLQHGDAFLHAVGRSWLRGRHVLGDADGEPRTG
                     VARMTSAPPS"
     misc_feature    complement(7084..7099)
                     /note="TFBS match to AfsQ1, Two-component system AfsQ1-Q2,
                     activator of antibiotic production, confidence: weak,
                     score: 16.86"
                     /tool="antismash"
     misc_feature    7813..7828
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.81"
                     /tool="antismash"
ORIGIN
        1 ccggcccggc tccccgcccc gcccgggacc ggccccggcc ggcgagacga gccgcgtacc
       61 agtgcgccgc ctcggcgaag cgggcctcga aacccggtcc cggatcctca ccgatccggg
      121 accacagacg gtcggcacgg taccagtggt cctgggtgag caaggcgtac tggtggtcgg
      181 agagctcggg caccgcacgc gcgagaagac cgcggtgctc cccgggcgac acatccccgc
      241 ggggcaccgg aaacccgagg tggcgcccga gcgccgacag gagccggtcc gccgccaccg
      301 gccgggggtg ggccgcgtgg tacaccgcgg aacggtccgc ggcgctcggc gcgcacgcca
      361 gacccgccac accgcgcgcc agatcaccca ccgcgatcag ggaactgcgc gaggcccggc
      421 cggccggcca cgccggcacc tcggtgagga gccgggccag agccgggatg aaccactcgt
      481 cgcccacgcc gtacaccaga tgcggacgca gcacggtacc gcccgcgtcc agcaccgccc
      541 gctccgcccg cagccgtgac atgctcgccg gagaagcagg acgcggcacc agcgcgtgtt
      601 cgtccgcgcc ccggtgcggc cccacaccat agacggacgc cgtgctgaca taaacgacgt
      661 gccccacacc cgcgcgccgg gcctcctcca gcagagcaag agtgccgcca tggttgaccg
      721 cctcgcaccg ggcggcatca cgccccacat aggcggcagc atgcaccact acctccaccc
      781 ccgccaacgc cccccgcagc gacccgggcc gggtcaggtc ggcgcggacg tgacccacca
      841 cgccatcacg cgacaacggc gcccggcgcg ccagcacccg caccgcgggc ccgccgcggg
      901 cggcggcgcg cgcggccagc tcacccacca ccgcgcttcc gatgaagccg ctgccgccga
      961 cgaccagaac ggtgccccta acaggtgacg gccaactcga cacnnnnnnn nnnnnnnnnn
     1021 nnnnnnnnnn nnnnnncttc cccccacttc gctcgaacgg cgacaccgag gcgggccggc
     1081 ccgcacgagg cagaatatac cggctggacg gttcttcggg gcggtccggt gtgggcggac
     1141 gggggcgggg gtgccgagcg gccgggccta cgcgcgcctg gccgccccgg cgtgcccgcc
     1201 gccgcaagcg gacggtcaag accgccggtg tccgcggcac gggccaggcc ctgacgcacg
     1261 gatcaatgga cctcgacaca cacgccgaca cgctggacac cccacggccg ggctcgcacg
     1321 gaaaccccgc tcaggccctg acgcgcgaac agatagacct cgacgcagac gccgacacac
     1381 tggacacacg tcacggccgg gctcgggcgg aaaccccgca gggacgctct gcggcgaaga
     1441 gccgcagccg gagagccgaa ccgcgcggcg cacagccccg ccgacgacac cacacgcgtg
     1501 tccgcacccc ctgctctccc cgcccccctg cggcgggccg gcgcgagagc tgcgaccacg
     1561 ccagagggga aagcgcggcc gccggcggac agccggccac attcgctgcc accgggggtg
     1621 ccgccccgct gcggtggagg acagcgtgtt cgtgcagccg cctgccgggg ccgttgtgaa
     1681 gccgccgcgt cccacccgct cgccccggca tctgccgcgg ccggcgcgta cgtgcgcttt
     1741 ccgcccgccg cggcagcacg cggcggcgtc gccgggtgtg ctccgccgga tcgggcagac
     1801 ccatggccct gaagggcacc ttcccttcag tccgcgggcc cgtggccggc gccggcgccg
     1861 gccgggggcc cgcctcagtt cgcccgggcc cccggcagca ggctcctgcc ggggcccgca
     1921 cgggatgtgc gggaggcgcc cgggcggacc gccacctgcg gatcgacggt gatcgtggtg
     1981 caccccagcg gggcggtgag ggcgcggagc gcggtgacgg agagcctgat ccacgggtgg
     2041 tcctgaccca gggccgccag cagccgtgcg caggaggtga aggcgacggc ggtacggcgg
     2101 ccgccgggag tgcggaagaa gcacgtcaca cagcagcggc ggtgcggccg gacgggtacg
     2161 tacagcaggt cggccgacgg acgtccaccg ggcgtggggt ccgcgtcgcg ccgggcatca
     2221 gccatggtgg tctccgctca cgccttgcgg ccttcacctc accggggtga agtcacgcgc
     2281 cgcgacgaac gcgggccggc gggccggtgc cgcgaagggt tcgcccgcgg tgttctcgac
     2341 gctgttgaac acgatgaaca cgttgctgcg cgggtacggc gtgatgttgt cgccggagcc
     2401 gtgcatgcag ttgcagtcga accaggtcgc cgaaccggcc ttgccggtga tcagccggat
     2461 gccgcccgcc tcgcacagcg cggtgacggc ctcgttcgac ggcgtccccg cgtcctgcat
     2521 ctgcagggac ttcttgtagt tgtccctcgg ggtggcgccc gcgcagccca ggaacgtcct
     2581 gtgcgacccc ggcatgatca tcaggcttcc gttggtggcg tggttgtcgg tgagcgcgat
     2641 cgacaccgac acggcgcgca tgcggggcag gccgtcctcg gcgtgccagg tctcgaagtc
     2701 ggagtgccag tagaagccgc tcgccccgaa accgggtttg acgttgatgc gggactgatg
     2761 gacgtacacg tccgagccga ggatctggcg ggccgctccc accacgcgtt cgtcacgcac
     2821 gagcgaggcg aacacctcgc tgatccggtg cacttcgaac accgaccgga tggcacggga
     2881 ggcgggctcg acgacggcgc gctcgtcgca gccaactgcc gggtcacgca ccagcctgtc
     2941 cagctcggcg cggaacaggg ccacctcgcc ggggctcagc agtgcctcga cggtcgtgaa
     3001 gccggcgcgg tcgacatccg ccagtccggc ccctcccagc gggccgggcg cggcgggtgc
     3061 ggaccacacc acggggtcac ggcgcggcat catcgcctcg gccgtgccgc gggtcggata
     3121 cacatcggtg ctcaaacggt ggtgatgcat cgtcgtcatg gtcgtcccca tgcctggtcg
     3181 acttccggat cccttttccc ggttcgcagt cccctttcag gcacccggcc cgggcggcgg
     3241 gtaggcgccc tcggcgtcgt gatcctcccg gccggtcagg ggcgggttga acacacacac
     3301 gcagcgcacg tcggtcttgg gccgcatcgt gtgccgctcg tggccgtcca gcagatacac
     3361 cgtccccggc gtgatccagt gcttctcacc actctcctca tcggtcagct ccgcctcgcc
     3421 ctcgacgcag aacaccgcct cgacgtggtg ggtgtaccgc atcgtcgtct gcgtaccggc
     3481 gtagagcgtc gtcacgtgca aggagaaacc cgcctgctcc cgggccagca cgagccgttt
     3541 gctctcccag gtgccggacg cggccctgac atgccggacg gtgtcctcga tctcctcaag
     3601 ggaacggatg atcacagctc atcccacctt ccgtgtgcgt cgccgggcac agccacaccc
     3661 gcgcccgtgt acgtgttcat gtgcgtgtcc gtgtgcatgt gcgtgttcgt gtgcgtgtgc
     3721 gtgtgcgtgt gcgtgttcgt gtgctcaacg ggggccggcc gaccgcgcgg gccgttcggt
     3781 tccccgcacc gcggacgcga acagcgcgag gccttcctcc agttcctgct cctcgatggt
     3841 caacggcggg aacagcttga cgacttcgtc cctcgggccg gacgtctcca gcagcaggcc
     3901 cagctcgaag gcacgggcgc acacggcggc ggcccgcgac ggatcggcga actccacgcc
     3961 ccatgccagc ccacggccac gcacctcggg cgcgacagcg ggccgggacg cggcgacggc
     4021 ccgcaagccc tgctccatct gctccccgcg ggccagggtc tgcttctgaa gccggccgtc
     4081 gcgccagtac tcctccagcg cggcggtggc ggtgacgaaa gccgggttga aaccacggaa
     4141 ggtgccgttg tgctcgccgg gccgccagat gtccagctcg ggacgcaaca gggtgagcgc
     4201 catcggcagt ccgtatccgc tgacggactt cgacagcgtc acgatgtccg ggacgatgcc
     4261 ggcctgttcg aaggagaaga acgcacccgt gcggccgcac cccatctgga tgtcgtcgac
     4321 gatcagcagc atgtcccgcc gccggcacag accggccagg ccgcgcaacc actccgagcg
     4381 ggccacattg atcccgccct caccctgcac ggtctccacg atcacggccg ccggcgtgtc
     4441 cagcccggac ccctcacccg ccagcagccg ctccaaccag gaaaacgccc cctccccgcc
     4501 gcacggcccc ccgtcgtacg gcatgggcat cgcgtgcgcc aggggcacac ccgcacccgc
     4561 acgcttgaaa gcgttgccgg tgacagccag cgagcccagc gacatgccgt ggaaggcgtt
     4621 ggtgaagaag acgacggctt ccctgccctt caccttgcgg gccagtttca gggcggcctc
     4681 cacggcgttg gtgccggccg gccccgggaa catcaccttg tacggcagtt cacgcggctc
     4741 cagcacggtc tcctggaaag tctccaaaaa cgtgcgcttc gcggccgtcg acatgtcaag
     4801 ggcatgcgtg acaccgtcac gctcgatgta gtcgacaagc gcacgcttga gcaccgggtt
     4861 gttgtgcccg taattcagcg ccccggcacc ggagaaaaaa tccagaaagg ggcggccgtc
     4921 ctcgccgtac atgcgactgc cgagtgcacg gtcgaacacc acgggccagc cccggcaata
     4981 actgcggacc tcggactcga gcctctcgaa cacatgagag gcaggacggg aaaccgtcat
     5041 gatgctcgct cctcacggca gaccaacaaa ccgtctacgt caggcgtctt cgcggccgcc
     5101 gcctcatcga agcagcggcc cgatacggta caggaactcg gcatcgtgcg cgtcggggaa
     5161 gaactccgcc ggaaacagaa ggtccctttc gagcggtgcc gcgtgccgtc gggcgaaaga
     5221 ccggaacagc ccctcggaag cggcgttacc cgcgctgatg gtcgtctcca ggtgccgcag
     5281 ggtgccgccc gccgcaagcc gggcggtgag atggtccagc atcgcaccgg ccgtgccccg
     5341 tccccgttgc ccccggtcca cggccacctg ccagacgacg agggtgtcgg gctgctcggg
     5401 gcggcggaat cccgtgacga accccaccgg cacatcaccg gactgggagg ccaccacgga
     5461 agtacgggcg aaatccctga accacaacag ataacagtag ggggaattca catccagcac
     5521 cccagaatcg cgggcgatac gccacaccgc agccccgtcc ttaaccgtgg gaaccgccat
     5581 aaccagtgtg tccaccgaac acacctcact ctcgctgcgc caaccaccac gcgcatgcat
     5641 caacctgcgc gtgaacggac cccgtccggc ccattccccg cgcccccgaa agacgaccgt
     5701 tcaaccgacg gatcaccgac tacggtgtct gccgcttcag ggttcagttg tccgcacgga
     5761 cgtaccaccg tgacacatcg ctacgatgct acgcccccca caccacacac gcccacacga
     5821 aaaacccctg tttaaaaaag aagaaaggtg aaacgaaccg gcaacgttcc cccgcagcca
     5881 ggcaatccag caacgcggac tcgacccctg cacgtcatcc caagcaacat ccagcgccgg
     5941 aacccgggac tgctgcagtc ccactctcgt agttggcgag ggatgagagt ctgtcaacgc
     6001 ggcttgatgg cccagatcaa gccgcgttca cagacctatc tgccgtgatc agagtgaacg
     6061 cctgccctct tctgcacggc gggcgggcta ccaccagcat tatcctgacc ggaacatcaa
     6121 cggccacgac aggaccgcgt cgcctgtcct atcgatgtgg ctccggccac gggggaattt
     6181 cgtcatcgac cctgatctcc tggcggctct gtggagagaa cggcatccgt cggcccctcc
     6241 ggtcgctcac acgtttcgca ccaggtacgc ggatcgccgg gttcgcttcc acagcctccc
     6301 cggctcgaag cgctatctgc agtccgggga cgagtacgcg atcgtgctgg accggtacaa
     6361 cacgatcctc gacgagttgt tcacggacac cgatgtgttc gcggtgacga tggactggtc
     6421 ggacacaccg accgggcccc cgggctactc gaccccacga ccgacactgc acccgggcag
     6481 catccgctgg tggaccgagt cggaccagga tgaccctgac ccggaattcc acacccacac
     6541 gcgcctgtac gccgaccggc gccggtggag tcacggctgt gccgacgggc tgctgcgagc
     6601 agtcgcggac gaagcgctcg cggaagtctt catcaccgac acgaaactac gccatatcca
     6661 ccatccctac gacggcggcg cagacgtcat ccttgccacc cccgttcgcg gctcgccgtc
     6721 ggcgtcgccg agtacgtgcc ggcccctcag ccaggaccgg cccaccgcgt gcaggaacgc
     6781 gtcgccgtgc tggaggacct ggaccgcgat ggtcgggtcg ttcaccgccg aggacagtgc
     6841 ctgggctgcg acgccggcca ggacccgcgg tgcgaaggcg gggtcccgct cgacggtccg
     6901 ctcggcgccc agcgcgaccg tcccggccag tcggcgcggt tcgggcgggg tgcggggctg
     6961 cccatggacc tcgagcagta cggcgctgcg aggaaggggc tcgcacctca tgctgcagcc
     7021 ccggtacgcg cggcgcgagg cgggccgtac ggccgaaggc agctactgtc accagttcga
     7081 caggtgacga ctgggtgccc atactcgtca ccaacctgac cggtcacgca gcagccctca
     7141 gacgacgagc gggcgcatgg gccaggtacg cggacccggg agcgcggcgg aggcgcagcg
     7201 cgggctgcgc ttctcgaaac tgcccgacag gatccgtccg gatcgagggc accgtggagg
     7261 agcgcttcgc cgatacccac ccttcggcgg ccgccgccgt caccccggac tggtggctca
     7321 tacgcatggg cggcatctga ccccgcgccc cggcccgtcc gtccggggtg cgcccggccg
     7381 gggtggcccg tccccgccct cacggaaggc cccccgtgct ccactgcaag acactcgtcc
     7441 gcctgacatc gctcgcggcc gcctgagcgg tcaccatcgg atgcttcctg ggcgtgttcc
     7501 agcccgcccg ccgccgcctc acgaggacgc ccggtcgtcc gccaccatcg gacgatcggg
     7561 cgcgccaaag ccttcacagc gcaccttttc ggagccggac ggggaagcgc cgggtaccgg
     7621 acgctcgccg acccggcgct gccgccccga gaacaacgaa tgaagaccgc cggcagggac
     7681 ggcgtccgat ggcgcgctgg aacggaagtg atctctccgg ctgtccgatc ggtcacgcga
     7741 acgacgccgc gcggagattg tcacgcggag gagcgggggt ggtgctggtc gcgccaggtt
     7801 cgaggcgaga ttccgtatgc ctgtttgaac actttgctga agtgggtcgc gtccacgaag
     7861 ccccatctcc gtcctatcgc cgcgatggtc tgcagtcgcc cgttcgggcc ggccagttcc
     7921 cgcctgcatg cggccaggcg gcgtgcacgg acccagtcgc cgagactgat gcccgaccgg
     7981 gacagcacag cgtagaggtg gcgtacggac atgttgtgtg cggcggcgat ccgtgccgcc
     8041 gtcagatcgg catcagccag gtgggacagg atgtattgag tgatccgcag gccgagtgtc
     8101 gcttccagcg gttccttggc caggccggag ttgccgtgct gggacgtcag gacggcacgg
     8161 aggagttcga tgctgggttc cacgacggcg tcggcatgct cgccctggtg caattcgtcg
     8221 ctgacggcca gctgggagaa gtaggggaag gcgaggcgcg caacggggtt gtcggatccc
     8281 agagggcgcg cggtgacgtc tcgtagtaaa cggtcgggga gcgcgagtgc cgcgcggggg
     8341 aaacggatga agcggtagtc gactccctcg tcgaagagat gtgtgtaggg ggcgatcgat
     8401 tggtagacgg ccagttcccc cggcctcagc aggcactcgc gaccgttctg caccaccacg
     8461 ctgctgccct tcatctgcac accgagagtg acagccggct cctcgtcctc ccgtgccagc
     8521 cgctcggtgc gacggagagc gaccgcggtc gcccgcgccg aacagatctt gatgggcccc
     8581 acggtgccga gccctatgcg gacggagatg tcctc
//
