LOCUS       NZ_BMTZ01000002        22494 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces variabilis strain JCM 4422 sequence02, whole genome
            shotgun sequence.
ACCESSION   NZ_BMTZ01000002
VERSION     NZ_BMTZ01000002.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces variabilis
  ORGANISM  Streptomyces variabilis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces; Streptomyces griseoincarnatus
            group.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 620811)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 156X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649935.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 04:37:37
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,743
            CDSs (total)                      :: 6,668
            Genes (coding)                    :: 6,496
            CDSs (with protein)               :: 6,496
            Genes (RNA)                       :: 75
            rRNAs                             :: 2, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 172
            CDSs (without protein)            :: 172
            Pseudo Genes (ambiguous residues) :: 0 of 172
            Pseudo Genes (frameshifted)       :: 61 of 172
            Pseudo Genes (incomplete)         :: 147 of 172
            Pseudo Genes (internal stop)      :: 11 of 172
            Pseudo Genes (multiple problems)  :: 42 of 172
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 00:23:51
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 544499
            Orig. end                         :: 566993
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMTZ01000002.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..22494
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="lassopeptide"
                     /region_number="1"
                     /rules="(PF13471 and Asn_synthase or micJ25 or mcjC)"
                     /tool="antismash"
     cand_cluster    1..22494
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /kind="single"
                     /product="lassopeptide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..22494
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[10000:12494](+)"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      10001..12494
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="10000"
                     /detection_rule="(PF13471 and Asn_synthase or micJ25 or
                     mcjC)"
                     /neighbourhood="10000"
                     /product="lassopeptide"
                     /protocluster_number="1"
     misc_feature    complement(453..467)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.67"
                     /tool="antismash"
     misc_feature    complement(457..470)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.06"
                     /tool="antismash"
     misc_feature    460..473
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.12"
                     /tool="antismash"
     misc_feature    complement(463..477)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.35"
                     /tool="antismash"
     misc_feature    483..498
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 19.43"
                     /tool="antismash"
     gene            complement(487..2253)
                     /locus_tag="IE148_RS05650"
     CDS             complement(487..2253)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016328286.1"
                     /locus_tag="IE148_RS05650"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="WP_246538866.1"
                     /transl_table=11
                     /translation="MLSFIRNVNGKPETREAQFAHAEFLGRADFRGSVFSEHADFKYTT
                     FEAGAEFDNVEFKGGATFYSSKFLTHTSFDRVKFHGSVTFALTTFTERVDFSGATFETL
                     ANFPECEFLTDSHFFGARFKHYASFASCLFGGKVDYTLAKFDGHADFSRTRFEDTATLR
                     SVTFRSRANFDTADFAGDVDLSSIFNDEAYFVSARFGGEVSLEGSLFRHNLHLVGAEFV
                     ASSTLGPFMCAGSIKLSGAKFSSPVTIMASTYRVEAQRTRWGSTASLRLRNAQVNLSGA
                     VFEFPFTILTEHDNFSLPDGRSLPDVSSSSGSRVAVESIRGVDAAHLLLSDVDLSRCLF
                     AGTVHLDQVRLEGDCRFDTAPDGLYWHKGRPTRFTPRHVLAEEHSWRASRASTIRGWNF
                     AVLGTGRNGPAQLAPVYRALRKSYEDNKNEPGAADFYYGEMEMRRHATATPRAERYLLT
                     SYWALSGYGLRALRATIWLIAAMSTTLLSLMLWGLPMDDPKQTSSGKITGVHVRLTTNT
                     PDPVNPKGPPLERLTSERFEKSLRVVVNSVIFRSSGQDLTTAGTYLEMLSRLSEPILLG
                     FAVLAIRGRVKR"
     misc_feature    complement(2179..2181)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(2289..2304)
                     /note="TFBS match to DasR, N-acetylglucosamine dependent
                     repressor, confidence: weak, score: 19.56"
                     /tool="antismash"
     misc_feature    2415..2432
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.91"
                     /tool="antismash"
     misc_feature    2458..2476
                     /note="TFBS match to HgtR, Hydrogen-dependent regulator,
                     confidence: weak, score: 17.89"
                     /tool="antismash"
     gene            2901..4106
                     /locus_tag="IE148_RS05655"
                     /old_locus_tag="GCM10010287_11310"
     CDS             2901..4106
                     /EC_number="3.1.21.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_process="GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004928412.1"
                     /locus_tag="IE148_RS05655"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11310"
                     /product="restriction endonuclease"
                     /protein_id="WP_246538867.1"
                     /transl_table=11
                     /translation="MIDAVYAGGTFGHSGDDPISKLVPGTGNQGGFRYAGSPAQGTVRL
                     AVLYTSGAAVDWPDHLDAETGTFTYYGDNRTPGRGLHETPRKGNVLLRDAFELSHGTAQ
                     QRASGVPPFLLFEKVAPGRSVRFRGLLAPGGPTLAPDDELSAIWRASGARRFQNYRARF
                     TVLDEASVPRTWIRHLLAGGDPLGGDCPEAWRAWVESRVYRPLLAPATTVIRSTAQQLP
                     SDPVGEAILSEIREYFRGRETEFEACAVAIWRLIAPATGTVDVTRPSRDGGRDAVGSYV
                     LGPSAAPISIDFALEAKCYGPGNSVGVREVSRLISRIRHRNFGVLVTTSHFNQQVQSEV
                     HEDGHPIALVCGRDITDALRQHGRTTPAAVKDWLQRSFPRTQAGLEQRLDRTAGQDPAR
                     GR"
     gene            4114..4914
                     /locus_tag="IE148_RS05660"
                     /old_locus_tag="GCM10010287_11320"
     CDS             4114..4914
                     /GO_function="GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950070.1"
                     /locus_tag="IE148_RS05660"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11320"
                     /product="HNH endonuclease"
                     /protein_id="WP_189363876.1"
                     /transl_table=11
                     /translation="MPVETSENDETRAPDGTPWWDLTIQPGTKAKPFGQEKRLAAWLWF
                     NKILGDTFTMRELRAALGPDIADNAEHLNRRLRELRKCDWSIPSQRTDSTLAQDEYRLK
                     AKGGQIWLPGERDKYKKFAPSARIRRLVMDQDGSRCRICGLGPDDTYYEDGERVRLTIG
                     HLVPQERLKSRGARDDLSNWRTECSRCNETVRDEVPDPEQLDEVLAGLKRLTAKEAGQL
                     LEWMRKGERPRSRVDQAYDRARRLATPDRDRVIGNLAERLGEKG"
     misc_feature    complement(4130..4145)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 18.33"
                     /tool="antismash"
     gene            complement(5613..6314)
                     /locus_tag="IE148_RS05665"
                     /old_locus_tag="GCM10010287_11340"
     CDS             complement(5613..6314)
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF012422.5"
                     /locus_tag="IE148_RS05665"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11340"
                     /product="response regulator transcription factor"
                     /protein_id="WP_189363877.1"
                     /transl_table=11
                     /translation="MTVNLFVVSDNAVSLAGLEAIVDQDEECALVGRATDDSDDLRRLS
                     DLAGTALHPDVVVLSEPSSSRTVSARVRHIASAFPDEASRPHTIVVAQNDDDEMIVSAL
                     RAGVNGYLSRLGSADELLRSLKVVARGGAAFCPSVAARMSRYFSAMQGLHDGAGFPGLT
                     SRELEILELLAGGMGNRQIARRLFLAEKTVRNYVSRIFAKLEVHDRATAVVMARDAGFG
                     EPAGELVRNGA"
     misc_feature    6736..6750
                     /note="TFBS match to OsdR, Development and stress
                     management regulator, confidence: weak, score: 24.27"
                     /tool="antismash"
     gene            6796..7929
                     /locus_tag="IE148_RS05670"
                     /old_locus_tag="GCM10010287_11350"
     CDS             6796..7929
                     /GO_function="GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1048: sensor
                     histidine kinase"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018958865.1"
                     /locus_tag="IE148_RS05670"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11350"
                     /product="sensor histidine kinase"
                     /protein_id="WP_189363878.1"
                     /transl_table=11
                     /translation="MGEGPRRRAIVLDAAAACAFTLLPQVSLLRPAVDGGTGGGLLWTL
                     SLAAGLPLAVRRLWPVPVFVLVLAAACGALAAGLGPASFLAAAYAVHTVATTRRRDPGV
                     SAVAVAGLCAAVAALLTLSGGQSYQGGSTAVQAVFGLLVLGATWAAGSAVRERRRSTRR
                     AIEHAAEQAKTEERLRIARDIHDVVTHSVGLIAVKAGIANHVVATRPEEAKEALAVIED
                     VSRRALRDMRATLTVLRGEDRSEAGDLRPARGLADLPALLETAEAAGVRVELRTRYDQE
                     PPEGVALAAFRIVQESLTNVLKHAAPTGCRVDVTARQGVLTVDVTDDGPGPGRRTTVPG
                     GGMGLVGMKERAAAHGGTLAAGPRPGGGFRVTATLPF"
     gene            8047..8949
                     /locus_tag="IE148_RS05675"
                     /old_locus_tag="GCM10010287_11360"
     CDS             8047..8949
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006136293.1"
                     /locus_tag="IE148_RS05675"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11360"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_199214817.1"
                     /transl_table=11
                     /translation="MLAERLTKRYGPATVVDDLSFTVRPGVVTGFLGPNGAGKSTTMRM
                     MLGLTRPDGGTATVGGRAYRDLTYPLRHVGALLETSAPHRGMTAVGHLSWLARSNRVPR
                     RRVDEVLDAVGLTEAARKRVGTFSLGMGQRLGLAAALLGDPPVLVLDEPVNGLDAEGIR
                     WLRELLRAKAAEGRTILVSSHLMAEMAQVADELIVISRGRLLAETSVSEFLGRHGRTFV
                     RVRTSEPLRAAQEFQAKGATSVRRAADGGLEVDGLPAGEVNRIAAAAGVPVEELSTHTG
                     SLEETFLKLVDDGGDPTHV"
     gene            8942..9694
                     /locus_tag="IE148_RS05680"
                     /old_locus_tag="GCM10010287_11370"
     CDS             8942..9694
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024141.5"
                     /locus_tag="IE148_RS05680"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11370"
                     /product="ABC transporter permease"
                     /protein_id="WP_189363879.1"
                     /transl_table=11
                     /translation="MSETLAAARAEFTKMRAVRATSVALLLFVAVSVFIAALGGWSAKG
                     AIESGNPGLRSDFTPEQAGLDGILYGQLALIVFGVLMMSGEYTSGMMRVSLLAVPRRGR
                     LYLAKTAVTAVAALAVALPVTVVSYLVSQLALGPHGSTLDADGVPRALAGAVVYLTLMS
                     LLAVGVAAAARSAVLPLAVLLPLVLVGSQILSVIGATKEVARWFPDRAGAQMLTVDSGD
                     ALTGLVVLLAWTAAALTAGWLRHRSWDG"
     gene            9811..9939
                     /locus_tag="IE148_RS05685"
                     /old_locus_tag="GCM10010287_11380"
     CDS             9811..9939
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_103079659.1"
                     /locus_tag="IE148_RS05685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11380"
                     /product="aborycin family tricyclic lasso peptide"
                     /protein_id="WP_106963308.1"
                     /transl_table=11
                     /translation="MTAIYEPPALQEIGDFDELTKCLGIGSCNDFAGCGYAVVCFW"
     gene            10001..11812
                     /locus_tag="IE148_RS05690"
                     /old_locus_tag="GCM10010287_11390"
     CDS             10001..11812
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Asn_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1177: asparagine synthase (glutamine-hydrolyzing)"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_067372303.1"
                     /locus_tag="IE148_RS05690"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11390"
                     /product="lasso peptide isopeptide bond-forming cyclase"
                     /protein_id="WP_189363880.1"
                     /sec_met_domain="Asn_synthase (E-value: 4.8e-39, bitscore:
                     130.1, seeds: 78, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEFTVLPDCPAGAALADRVAAPKRIDHASGRPWIVGDWPEGGTTV
                     TEAGTRRLAVFGHTRPDDAGTASALGRMRSLHDVDRVASRLPGVFHLAASLDGAVRLQG
                     SVAGVRQVFTARVDGVTVAASAVDPLLRLTGAGLDETLLAARLLAPGGAPWPLSPRPVR
                     RGVDALPTGHWLELGADGRARSVRWWELPEATLSLEEGAGAVRSALTDALATRVDPHRT
                     VSADLSGGLDSTTLCFLADAAGADLVTYHVMPLDEANEDTAWARKAAAHLPHARHHMLA
                     ADRAANLFDIGYTADTVNAAPEGPSTWASGLAHIRDLAGRATAEGAALHLSGFGGDELF
                     GRMPACAWSLARLRPADGLRLVNRYRLANRWPWRSTLRQLADRSTFAQNLTEVAGRITA
                     PPPPVNEPDFGWVFAPRMPAWATPDAVAAVRDLLTTAAERTPEPLDADRARHQALSSIV
                     FEGNTVRQVNTAVAGTGLVWEAPFLDDRVLEAAFATRIDERLAAGRFKPLLTTAVRGLV
                     PDDFLARRDKGEFSAETFRGIERNRDRILDLCEDSLLARLGLVDPHAFRSAVLNPGPMS
                     HHLQPIQTTVACESWLRAHPQDTGENR"
     aSDomain        11809..11979
                     /aSDomain="Lasso_Fused_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="RRE-containing fused peptidase in a lasso
                     peptide cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IE148_RS05695_Lasso_Fused_RRE.1"
                     /evalue="4.50E-09"
                     /identifier="RREFam003.1"
                     /label="IE148_RS05695"
                     /locus_tag="IE148_RS05695"
                     /protein_end="57"
                     /protein_start="0"
                     /score="27.3"
                     /tool="antismash"
                     /translation="MKLSLARDVTLTPVDSGAVLLDGRRGRYYQLNASGSAILHKLLDG
                     DTPAAAAASLSE"
     gene            11809..12072
                     /locus_tag="IE148_RS05695"
                     /old_locus_tag="GCM10010287_11400"
     CDS             11809..12072
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: Stand_Alone_Lasso_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lasso_Fused_RRE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF05402"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF033530.1"
                     /locus_tag="IE148_RS05695"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11400"
                     /product="lasso peptide biosynthesis PqqD family chaperone"
                     /protein_id="WP_037640857.1"
                     /sec_met_domain="Lasso_Fused_RRE (E-value: 9.1e-08,
                     bitscore: 27.3, seeds: 20, tool: rule-based-clusters)"
                     /sec_met_domain="Stand_Alone_Lasso_RRE (E-value: 4.2e-23,
                     bitscore: 76.4, seeds: 25, tool: rule-based-clusters)"
                     /sec_met_domain="PF05402 (E-value: 5.6e-13, bitscore: 44.2,
                     seeds: 461, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKLSLARDVTLTPVDSGAVLLDGRRGRYYQLNASGSAILHKLLDG
                     DTPAAAAASLSESAPVSEERVHQDVLALVRSLSEADLVEVTQ"
     aSDomain        11815..12063
                     /aSDomain="Stand_Alone_Lasso_RRE"
                     /aSTool="RREfinder"
                     /database="RREFam.hmm"
                     /description="Stand-alone RRE protein in a lasso peptide
                     cluster"
                     /detection="hmmscan"
                     /domain_id="rrefinder_IE148_RS05695_Stand_Alone_Lasso_RRE.1
                     "
                     /evalue="2.10E-24"
                     /identifier="RREFam002.1"
                     /label="IE148_RS05695"
                     /locus_tag="IE148_RS05695"
                     /protein_end="85"
                     /protein_start="2"
                     /score="76.4"
                     /tool="antismash"
                     /translation="LSLARDVTLTPVDSGAVLLDGRRGRYYQLNASGSAILHKLLDGDT
                     PAAAAASLSESAPVSEERVHQDVLALVRSLSEADLVEV"
     gene            12069..12494
                     /locus_tag="IE148_RS05700"
                     /old_locus_tag="GCM10010287_11410"
     CDS             12069..12494
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     lassopeptide: PF13471"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016469533.1"
                     /locus_tag="IE148_RS05700"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11410"
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="WP_048459500.1"
                     /sec_met_domain="PF13471 (E-value: 3.7e-26, bitscore: 86.6,
                     seeds: 84, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTPAVAEQATRLPLHRQIAPRCAAGAARLLVRLPPARLHRVLRV
                     LSKGSRPAGYAQVARARRSVVSVSTRCAGLGCLQRSVATVLLLRVRGRWADWCTGFRVQ
                     PFAAHAWVEAGGRPVDEPGEVGVFRTVLAVRRTGGGS"
     gene            12491..13456
                     /locus_tag="IE148_RS05705"
                     /old_locus_tag="GCM10010287_11420"
     CDS             12491..13456
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014056405.1"
                     /locus_tag="IE148_RS05705"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11420"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_086700784.1"
                     /transl_table=11
                     /translation="MTAIRAEGLYAYYGTTPAVNGLHLNVPEGATFGFLGPNGAGKTTT
                     ISMLTTLLRPTAGRAEVAGFDVTTHAAEVRRRIGIVFQESTLDLELTATENLRFQADLC
                     GLGRAEARAAVAAMLDMMELTGRDRTPVRHFSTGLRRRLEIARGLLGSPRVLFLDEPTT
                     GLDTQTRAAVWHHLDRLREEQGITVFFTTHQLEEAEHCDRIAIFDRGKLVTEGSPAELK
                     SVIGADVVDLRTDDDRLAVDLLSDRFGLTAENTPGGLRLRVQDGASMVPRLCTGLGLGV
                     RSVTVTPPSLDDVYLHHTGTAIRDSGSDARSLDSLGEGLR"
     gene            13453..14322
                     /locus_tag="IE148_RS05710"
                     /old_locus_tag="GCM10010287_11430"
     CDS             13453..14322
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1065: ABC-2 type
                     transporter"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020123205.1"
                     /locus_tag="IE148_RS05710"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11430"
                     /product="ABC transporter permease"
                     /protein_id="WP_086700783.1"
                     /transl_table=11
                     /translation="MTRTDIPPSVTVTKPAPPSSEGRTWHTVRPYALLWRREMTRLRHN
                     PLRLVMGLVTPLLFLVVLGTGLEAASSTLGKAQLNDYRAYLFPGALVMSVQAPAIAVGI
                     SLVWDRRLGMLRQMLVSPFPRSSIVLGLALGGATTGAVYGLALLAVGGIAGVRYTPMLL
                     VVLVELLLVSLLFTSFGLLAAVTIRQVDTFQIVVNLSLMPLMFFSGAMFPPNGLPGWLD
                     TVVKLNPLTYGVDAVRRTLPGPDVLTSEQTRLMLGDWNPPVYAELGVLAALTAAVLGLA
                     TYRFSRAQ"
     gene            14336..14809
                     /locus_tag="IE148_RS05715"
                     /old_locus_tag="GCM10010287_11440"
     CDS             14336..14809
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF018942.5"
                     /locus_tag="IE148_RS05715"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11440"
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="WP_086700781.1"
                     /transl_table=11
                     /translation="MTFVRTWAGTAARIVLAAVLGYAGWVKVQDLTGAGRTVALYQLVP
                     EESAQLVGAAVAFVELALALLLLAGLATRAVAAVTALLMVTYIAAIASVWARGMSIDCG
                     CFSSGGTLTGGAERGYVVDIARDLAFLGAAAFLITRPRTRYALDRWVLETKER"
     gene            14812..15537
                     /locus_tag="IE148_RS05720"
                     /old_locus_tag="GCM10010287_11450"
     CDS             14812..15537
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024853.5"
                     /locus_tag="IE148_RS05720"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11450"
                     /product="thioredoxin domain-containing protein"
                     /protein_id="WP_048459504.1"
                     /transl_table=11
                     /translation="MQPSTDAQVREMVHRRRRRRRTVLVSLAAVVTVLGAALVGAGLVR
                     ATDTEPGDAPEEVPAGVAADRAGLVTSEGPVRVDLYLDYLCPECRITEKALAPELREMQ
                     ERGEVRVVHHPVAFLDDRSAPAGYSTRAASAAACAADRNKFEPYTAALFDEQPPEQGPG
                     LDTDRLVALGRDVGITGASFERCVRDGTYRPWVTYVSDVAASRGVALTPTVKVAGKRVD
                     LSGPDRAKAFVRAVEESRS"
     gene            15534..16856
                     /gene="mslH"
                     /locus_tag="IE148_RS05725"
                     /old_locus_tag="GCM10010287_11460"
     CDS             15534..16856
                     /codon_start=1
                     /gene="mslH"
                     /inference="COORDINATES: protein motif:HMM:NF038314.1"
                     /locus_tag="IE148_RS05725"
                     /note="Involved in the biosynthesis of MS-271 and related
                     lasso peptides; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11460"
                     /product="lasso peptide C-terminal Trp epimerase"
                     /protein_id="WP_086700778.1"
                     /transl_table=11
                     /translation="MSRLTVALTGDCMMTRGAPVTSDPAAGRLGEVLHTADFAFTNLEV
                     APATGRGHPVPDAASGGGLIADPAVLDDVTAMGFSALSCANNHALDLGTEGVLGTVDLL
                     RAKGVPFAGIGADLTAARRPVYVDRPAGSLALIACTATFRDGQEAAAPSPDLPGRPGLS
                     PLRHGVTLRVTAAQLEALREIDGATGLRSRRAEARTLLGFDPAMPGPGRLALFGTRFET
                     ADTPGLTTACDPRDVHDITRWVAEARRRADLVLVSVHSHEPGPTPETPAEFLRDFARAV
                     IDAGADAVVGHGPHMLRGVELHRGRPVFHSLGNIVSQIELADRIPAEDYARAGTDESLT
                     PGGYFERITAHGRRMFAPHRRYWQSLVPVLTFDDGVFTGARLHPVDLGFGQPVHRRGRP
                     RLAGPVDAKEILTDFARLAEPHGVTVTVGDDGTGELVAAET"
     gene            16956..17633
                     /locus_tag="IE148_RS05730"
                     /old_locus_tag="GCM10010287_11470"
     CDS             16956..17633
                     /GO_process="GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1016: LuxR family
                     DNA-binding response regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942992.1"
                     /locus_tag="IE148_RS05730"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11470"
                     /product="response regulator transcription factor"
                     /protein_id="WP_086700777.1"
                     /transl_table=11
                     /translation="MAEAVRVLIADDQALLRGSFRVLVDSTPGMETVGEAGDGAEAVAL
                     ARELTPDVVLMDLRMPVMDGIAATREICSDASMAGVRVLALTMFDMDEYVYPALQAGAS
                     GFMLKDASPDDLVAGIRVIATGEGVLAPSVTRRVIANFSHRDTAARPAPRLVGLTRREQ
                     EVLVLIAKGLSNAEIADRLVISLPTVKTHVSSLLSKLNARDRAQLVIVAYESGIAERGM
                     PPA"
     misc_feature    complement(16957..16971)
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: strong, score: 19.46"
                     /tool="antismash"
     gene            complement(17650..18000)
                     /locus_tag="IE148_RS05735"
                     /old_locus_tag="GCM10010287_11480"
     CDS             complement(17650..18000)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE148_RS05735"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010287_11480"
                     /product="hypothetical protein"
                     /protein_id="WP_122215948.1"
                     /transl_table=11
                     /translation="MVTGAELVATALTTGAAAGLTGTARGTVHDLHDALRQAVRRRLTA
                     PDGTGGTYGVRVLDAHATDPDVWGTRLLRVLVACGAGEDEDVLRAARALLRAERGPAGR
                     ATDASLRRSSLP"
     gene            18107..21067
                     /locus_tag="IE148_RS05740"
                     /old_locus_tag="GCM10010287_11490"
     CDS             18107..21067
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1041:
                     transcriptional regulator, SARP family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019526516.1"
                     /locus_tag="IE148_RS05740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010287_11490"
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="WP_189363881.1"
                     /transl_table=11
                     /translation="MGGESQRLRIEVLGPLRAWRDGGPLALGPVRRQTVLAALLLRDGA
                     VVSHEQLLDGVWGADPPASGTKVLASHVNQLRRTLDRAGTRHTESVIRSGKGWYRFVLD
                     DVRLDLADLAELGDRGLRTKASGDLGGAAEQLTEALALFQGEPLAQLSGPFAAAERERL
                     SERRRTLRLARLSCLVDLGRYGDALDDLAPLPEADPYDESVLALRMRALYGCERQAEAL
                     HAYEDMRRRLADELGVDPGEELRRIHEAVLRQDDAALLGATERQVVTVQPPARRHLVNQ
                     LPGDTGQLVGREDELSVLTAPGPDDAVSVVAVDGPAGVGKTALVVRAAHRIRDRYPDGC
                     LFVDLHAHSRERGRPAPESVLRRLLRALGADGSEVPDDLDDLMTAWRTVTSPLRLLLVL
                     DDAAGTRQIRPLLPAGPGSRVIVTGRQRLAGLDADRRATLETLGPGEAASLLRQIVGRE
                     RTDREPEATRDLVRLCAGLPLALRIVGSRLQTRPAWTLAYLVERMADDEGRLGELSTGD
                     RSVEAAFRLSYDQLPPEQRRGFRTLGLAPTVEFDVRTPAAMLRRPARDAERILESLVDT
                     SLLQQPRPGRYRMHDLVRVHARRLAEAAHAEAGPAREAALRLYLDAARVASDWGAGGYP
                     TGPGRSGASFSGWREAAVWLDEAGGELVDVVGHAVASGEVDHACWIAEALTDHFVGRGR
                     LHECQAALETALPYVDAAADRRMAPALHNCLGYTLTHRARFPDACRHLRTALDLSRERG
                     ERHEEGRALVGLGAMTGLGAAGPDADEVEEGVSVLVAGRELAEGYDDHWLVAMASFALG
                     FVRHRQGRDEEALACFRDAHTRGRAIGRPRALSRALTSNLDLHLYLDPSAARVLFRRVA
                     DLIRRTGDVLLHTLTLARLGTAEHKAGNLDAAAEVFRRALAQHGTPGPLDDPDHDRREM
                     DLRCRLGRTYRAAGRPAEAREQFRVALAVPGADRYPKEHKQAVEGLAATEVPSRAG"
     misc_feature    complement(21223..21240)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.41"
                     /tool="antismash"
ORIGIN
        1 cgtaactgcc acgtggtgca gtctcaccac gtggtgctcg aaccgctcca tcccgggtgg
       61 ctccacgtcc catagctgaa gcttgaccca ccggttgtcg tacaaatcgc gctcaccgtg
      121 gatgcgccac ggcgccagcc cttctggacg ttcgacgctc actgcgcccg gcactcggta
      181 tgaactccgc cctcggacct ccagggcccc ctcggcgacg aggcgagcga gtacctgccg
      241 aagtgcggtt cggccgatgc cgagttcttc gaccaatgca cgctctgagg ggagcttgtc
      301 accgggggcg tacttgctcg atgcgagcca tgcacgaagc gtctcataga ccttagttgt
      361 cttcggtccc atccgcagag cactctccat tccgaagtgg tgcgtaccag agtagcgtgc
      421 gctggctttc gcggcctccc tctcggtata tgaaatttat gtaatttata gaaggttcaa
      481 aattctctac cttttcactc tgccacggat agccagaacg gcgaatccaa gaaggatagg
      541 ctctgagagg cgagacaaca tctccaggta agttccagca gtagttagat cctgccctga
      601 tgaacgaaat attacggagt ttacgacgac tctgagactt ttttcaaagc gctcagatgt
      661 gagtctttca agtggaggcc ctttaggatt tacagggtct ggtgtattag tcgtgagtcg
      721 aacatgcacc cctgtgatct tcccgctaga cgtttgcttg ggatcgtcca tagggagtcc
      781 ccatagcatt agcgagagca gtgtggtgct catggctgca atcagccata tcgttgcgcg
      841 aagagcccta agcccatagc ccgatagggc ccaataagat gttagcaaat atctctccgc
      901 gcgaggagtt gctgtagcgt gccgacgcat ctccatctcg ccgtaataga aatctgccgc
      961 tccaggctcg ttcttgttgt cttcgtatga cttcctgaga gccctgtaga cgggtgctag
     1021 ttgtgctggc ccattccttc cagttcccaa cactgcgaaa ttccagccgc gaatagtgct
     1081 ggccctgcta gctctccaag aatgctcttc cgcaagtaca tgccttggag tgaacctggt
     1141 tgggcggcct ttatgccagt agaggccatc tggcgctgtg tcgaatcgac agtctccttc
     1201 taggcggact tgatctaggt gcactgttcc tgcaaatagg cacctggaga ggtccacatc
     1261 agaaagtagc aagtgtgcgg catcgactcc tcttatggat tcgactgcaa cgcgtgatcc
     1321 acttgaggat gatacgtcag gcaggcttct tccgtcagga aggctaaagt tgtcatgctc
     1381 ggtaagtatg gtgaatggaa actcgaacac ggcacctgaa aggttcacct gcgcgttcct
     1441 cagcctcagg ctagctgtgg agccccaacg tgttcgttga gcttctacac ggtaagttga
     1501 cgccattatc gtcacagggc tactgaattt cgccccagaa agcttgatgg atcctgcgca
     1561 cataaaagga cccaatgtgc tagatgctac aaattcagcc cctactaggt gcaagttatg
     1621 gcggaacagg gaaccctcta gggagacttc gccgccgaac cgagctgaca caaaataagc
     1681 ctcgtcgttg aatatagagg agaggtcgac gtcgccagcg aagtcggctg tatcgaagtt
     1741 ggccctcgat cgaaaggtga ctgagcgtag agtggcggtg tcctcgaagc gagttcgtga
     1801 aaaatcggcg tggccgtcga acttggctag tgtgtaatcg acttttccgc cgaataggca
     1861 acttgcgaaa ctggcatagt gcttgaatcg agcaccaaag aagtgagagt cggtcaggaa
     1921 ttcgcattct gggaagttgg ccagcgtctc gaatgtggca cccgaaaaat caactctttc
     1981 cgtgaaagtt gtgagggcga acgtgaccga gccgtgaaac ttcacgcgat cgaatgatgt
     2041 gtgggtcagg aattttgagc tatagaaagt cgcgccacct ttgaactcga cattgtcgaa
     2101 ctctgcgcca gcttcgaagg tggtgtactt gaagtccgca tgctcggaaa atacgctgcc
     2161 cctgaagtcg gcccgtccta aaaactctgc atgcgcgaac tgggcctctc ttgtttctgg
     2221 ttttccgtta acgtttcgaa tgaagctaag catgactcgc aagcgggctt cagttatgcg
     2281 ggttccgcgg tggtctaggt ctgtacccgg tctgagtgag tggcaataac tggcgaattc
     2341 atcgtcgtca agatgttcta ggcatgcgtt gacgggggat acggcgcgcc ctgggcaggc
     2401 gatgattcct gacgatgttg agcggtcgta accgacacat tgcggccaat caggatctgt
     2461 ctggatactg ttcacatcgg acttagacat ccggtggccc gtcgtggttg cgctgatccc
     2521 ttgatggcag aggcatggca cctctcgcga agtgtccagc tgtcgcgcta ccggggagtg
     2581 ctaagcggca cactcctctt cgctgtgggg gccgccgtgt ctcagttgcg gtctcgattc
     2641 acacaagacc ccgtggcgtt cgttgatgtc cgtgggtgtc catcaccacg cctggccagg
     2701 gcactctgcg gtgacgcgga cacgcgtgga cgtgcgcgga ccaagatcgg acaacttgta
     2761 atgtgcgcct ctggctcggg atgcgttatg gcggatcatc cgtgttcggg cactgcgttt
     2821 gatagacgta caccatgatc cctgcgcccc gctcagctga gccgagtacc cggttcgagc
     2881 accttggtga cgctgacctg gtgattgacg cggtctacgc cggtgggacc ttcgggcact
     2941 ctggcgatga ccccatcagc aagctggtcc ccggtaccgg caaccaggga ggatttcgct
     3001 acgccgggtc gccggcgcag gggaccgtcc gcttggctgt gctgtacacc agtggcgctg
     3061 ctgtggactg gccggaccac ctggatgcgg agaccggtac cttcacttat tacggcgaca
     3121 accggacccc gggtaggggg cttcacgaga cgccgcgcaa gggtaacgtg ctgctgcgcg
     3181 atgccttcga gctctcacat ggcaccgcac agcaacgggc cagcggcgtg ccgccgttct
     3241 tgctcttcga gaaggttgcg cctggccgga gtgtccgttt ccgcgggttg ctagcgcccg
     3301 gggggccgac tctggcgccg gacgatgaac tctccgccat ctggcgggca tctggcgccc
     3361 gccggttcca gaactaccgc gcccgcttca cggtgttgga cgaagccagt gtcccgcgta
     3421 cctggatccg ccacctgctg gcgggtggag atcctctcgg gggcgactgt ccggaagcct
     3481 ggcgggcctg ggtcgaaagc cgtgtctaca ggccgctgct ggcccctgct acgacggtca
     3541 tacgctcgac agctcagcag ctgcccagcg atccggtcgg cgaagccatc ctgtcggaaa
     3601 ttcgggagta cttccgcggg cgcgaaacag agttcgaggc ctgtgccgtg gctatctggc
     3661 gtctgattgc gccggccacc gggacggtgg acgtgacacg ccccagccgg gacggtggcc
     3721 gcgatgccgt cggctcgtac gtcctcgggc cgtcggcagc tcccatcagc atcgacttcg
     3781 ccctggaggc caagtgctat gggccgggca acagcgttgg ggtcagagaa gtctcccggc
     3841 tcatctcccg aattcgacac cggaacttcg gagtcctcgt caccacctcg cacttcaacc
     3901 agcaggtcca gagcgaggta cacgaagacg ggcatccgat cgccctggtc tgcgggcgtg
     3961 acatcacaga cgcgctccgc cagcacggca ggaccactcc cgcggcggtg aaggactggc
     4021 tgcaacggag cttccctcgg actcaggcgg gcttggagca gagactcgat cgaaccgctg
     4081 ggcaagatcc tgcgcgtggc agatgattgg catatgcctg tcgagaccag tgaaaacgac
     4141 gagacccgag cgcctgacgg gacgccgtgg tgggacctca ccatccagcc cggaacaaag
     4201 gcgaagccct tcggccagga gaagcgcctc gccgcctggc tgtggttcaa caagatcctg
     4261 ggtgacacgt tcacgatgcg ggagctgcgt gctgccctgg ggccggatat cgccgacaac
     4321 gcagagcacc tcaaccggcg cctgcgagaa ctccgcaaat gcgactggag catcccctcc
     4381 cagcggaccg acagcacctt ggctcaggac gaataccggc tcaaggctaa gggcggacag
     4441 atctggcttc ctggtgagag ggacaagtac aagaagttcg cccccagcgc ccgcatccgc
     4501 cgcctggtca tggaccagga cggttcgcgg tgcaggatct gcgggctggg gcccgacgat
     4561 acgtactacg aggacgggga acgggtccgg ctcaccatcg gtcatctggt tccgcaggag
     4621 cggctgaagt ctcgaggagc ccgggacgat ctcagcaact ggcgcactga gtgctcccgc
     4681 tgcaacgaga ctgtgcgtga tgaggtcccc gacccggaac agctcgacga agtcctggct
     4741 gggctcaagc ggctcaccgc caaggaagca ggacagcttc tcgaatggat gcggaagggc
     4801 gagcgaccgc ggtcccgggt cgaccaggca tacgaccgcg cacgccgact ggctacgcca
     4861 gaccgtgacc gagtcatcgg aaatttggcc gagcgcctcg gcgagaaggg ctgaccacca
     4921 ggccgtacgg ggccgcagca cggttgcagc aggacgcaga ggcctcggac cagttccggg
     4981 gccttctccg tgtagagagc agcgaggcgt tacggttcgg cgcatgggga ttccaaggtg
     5041 ggccggtcgc gcgacggccg cagtggtcgt ggccggggcc ttgatcattc ggcgtgcgct
     5101 tcagaatgct gcggcgcagt cagagtccgg ctcagactcc gggtacgact ggaaccagaa
     5161 cgagcccaat tggcactggc gttgcgagtg cggcgccaaa tctcgtggcg gcgatatcaa
     5221 ggctgacacg gagtacaacg cccagcggca ccagtggcgc gcaggtacgg ggcacccgat
     5281 gcctgaggtc tatcagaagc ccttgtaggt cgtcgccgct gggagctgtc gacgggcgac
     5341 gctggaggct tgttgccgaa ggggacgaag gcggcgcggt ctcagttgcg gtctcgcttg
     5401 ccacccgttc cgggtctgtc cgctcatgtc caccagggct tgacaaccct cgtgaccagg
     5461 cgcggagccg actccacaga cgctcgcgga cgcccccgga ccaagatcgg acaactcgta
     5521 atgcgtaggt ctcgggttcg aatcccgaag gcggctcttc ttcaagcccc gcgcagacag
     5581 tgtctgagcg gggcttcctc gttccggtgc gctcacgccc cgttccggac cagttccccg
     5641 gcgggctcgc cgaagcccgc gtcccgggcc atcaccaccg ccgtcgcccg gtcgtgcacc
     5701 tcgagcttgg cgaagatgcg cgagacgtag ttgcggacgg tcttctcggc caggaagagc
     5761 cggcgggcga tctgccggtt ccccatcccg ccggccagga gctccagtat ctccagctcg
     5821 cggctggtga gccccgggaa cccggcgccg tcgtgcaggc cctgcatggc ggagaagtag
     5881 cggctcatgc gggccgcgac gctggggcag aaggcggcgc cgccccgggc gacgaccttc
     5941 agggagcgga gcagttcgtc ggccgaaccg aggcgggaga ggtagccgtt gacacccgcg
     6001 cggagggccg agacgatcat ctcgtcgtcg tcgttctgcg ccaccacgat cgtgtgcggc
     6061 cgggacgcct cgtcggggaa ggcggaggcg atgtgccgga cgcgtgcgga gacggttctg
     6121 ctggaggacg gttcgctgag gacgaccacg tcggggtgga gggcggtgcc ggccaggtcc
     6181 gacagacgtc ggaggtcgtc cgagtcgtcc gtggcacggc cgacaagagc gcactcctca
     6241 tcctgatcga caatcgcttc gagcccggcg agggacaccg cgttgtcgct gaccacgaac
     6301 aggttgacgg tcatctcgta catcccccag aaatgcgtcg acgtggtgcg gacggcgccg
     6361 gaaggtcccc gggggcacac gcgacgaagg gtcggtccgt aggcgaccga ctccgggcgt
     6421 gacgtggtca gttgtggttt tcagcggccc ccgtggccat gacctgtcgg cgcaggcatg
     6481 gtggtgcatc gaaagcgcgc aaggcgctcg tggtgccgag tatgtggacg cgtccggaga
     6541 gaacgcaagg tcgccaacgc acccgtgtcg tccgaggggt gagtatcggc cggatcccgc
     6601 cgaaccaccg cgattccggc gtcggtggtg gtgggggcgg ctccgggcgc gcgacccgca
     6661 ggaccgcgcg cccgtgtggg ccgtggtgcg gggcggacga cactgtccga aacggtcacc
     6721 gcccggcgcg acacgcggac gtccggccct gcgcccgttg gacgggccgt ggagcgagcg
     6781 cttacgttcg acggcatggg tgaggggccc cggaggcgtg cgatcgtgct ggacgcggcg
     6841 gcggcctgcg cgttcacgct cctgccgcag gtgtcgctgc tgcggccggc cgtcgacggc
     6901 gggacgggcg gcggcctcct gtggacgctg tccctggccg ccggactccc gctcgccgta
     6961 aggcggttgt ggcccgtccc ggtcttcgtc ctcgtcctgg ccgccgcctg cggggcgctg
     7021 gccgccggcc tcggacccgc ctcgttcctg gccgccgcct acgccgtcca cacggtggcc
     7081 accacccggc ggcgcgaccc cggggtgtcg gccgtcgccg tcgccgggct gtgcgcggcg
     7141 gtcgccgccc tcctcacgct gtcgggcggg cagtcctacc agggcggcag caccgccgtg
     7201 caggccgtgt tcggcctcct cgtgctcggc gcgacctggg cggccggctc ggcggtcagg
     7261 gaacgcagac gcagtacccg gcgcgccatc gagcacgccg ccgagcaggc gaagaccgag
     7321 gagcgcctgc gcatcgcccg cgacatccac gacgtcgtca cccacagcgt gggactcatc
     7381 gccgtcaagg ccggcatcgc caaccacgtg gtggccaccc gccccgagga ggccaaggag
     7441 gccctggcgg tcatcgagga cgtcagccgc cgggcgctgc gcgacatgcg cgccaccctg
     7501 accgtgctgc gcggtgagga ccggagcgag gccggggacc tgcggcccgc ccgcggcctc
     7561 gcggacctgc ccgcgctgct cgagaccgcc gaggcggcag gcgtccgcgt cgaactgcgc
     7621 acccgctacg accaggaacc gcccgagggt gtcgcgctgg cggcgttccg catcgtccag
     7681 gagtccctca ccaacgtgct caagcacgcc gcgccgaccg gctgccgggt ggacgtcacg
     7741 gcgcggcagg gcgtgctgac ggtggacgtg accgacgacg gccccggccc cggacgccgg
     7801 accacggtgc ccggcggcgg gatggggctg gtcggcatga aggagcgggc cgccgcgcac
     7861 ggcggcaccc tcgccgccgg cccccggccc ggcggcggct tccgggtgac ggccaccctg
     7921 ccgttctagg acctgacgga cgccggctca tcctcgggta tgaggggcgc ccgcgcggca
     7981 ccgcgccggg ttcccgaccg cggaccgatg cggtcgcggg gtgcgcgccg cgagactcga
     8041 cacgtgatgc ttgccgagag actgaccaag cggtacgggc ccgccaccgt cgtcgacgac
     8101 ctgtccttca cggtgcgacc aggtgtcgtc accggcttcc tcgggcccaa cggcgccggg
     8161 aagtccacga cgatgcggat gatgctcggc ctgacccgtc ccgacggcgg cacggccacc
     8221 gtcggcggac gcgcctaccg cgacctgacc tacccgctcc ggcacgtcgg cgcgctcctg
     8281 gagacgtcgg ccccgcaccg tggcatgacc gccgtcggcc atctgtcgtg gctggcgcgc
     8341 agcaaccgcg ttccccgccg gcgggtggac gaggtgctcg acgcggtcgg tctcacggaa
     8401 gccgcccgta aacgggtcgg caccttctcc ctgggcatgg gacagcgcct cggcctggcg
     8461 gctgccctcc tcggcgaccc cccggtcctg gtgctcgacg agccggtcaa cgggctcgac
     8521 gccgagggca tccggtggct gcgcgaactg ctgcgcgcca aggcagccga gggccgcacc
     8581 atcctcgtct ccagccatct gatggcggag atggcacagg tcgccgacga gctgatcgtg
     8641 atcagccggg gccggctcct cgccgagacc agcgtgtcgg agttcctcgg acgccacggc
     8701 cggacgttcg tacgggtccg gacctccgaa ccgctgcggg ccgcgcagga gttccaggcg
     8761 aagggcgcca cctcggtgcg gcgggccgcg gacggcggcc tggaggtgga cggcctgccg
     8821 gcgggcgagg tgaaccgcat cgccgcggcg gccggtgtcc ccgtcgagga actgagcacc
     8881 cacaccggct cgctggagga gaccttcctc aagctcgtcg acgacggagg agatcccacg
     8941 catgtctgag accctcgcgg ccgcgcgggc ggagttcacc aagatgcggg cggtgcgcgc
     9001 cacgtccgtc gcactgctgc tgttcgtcgc cgtcagcgtg ttcatcgccg cactgggcgg
     9061 ctggtccgcc aagggcgcga tcgagtccgg caatcccggg ctgcgctccg acttcacgcc
     9121 cgagcaggcc ggcctggacg gcatcctcta cggccagctc gccctgatcg tgttcggggt
     9181 gctgatgatg tccggcgagt acacctcggg catgatgcgg gtctcgctgc tcgcggtgcc
     9241 ccggcggggc cggctctacc tggccaagac ggccgtcacc gccgtcgcgg ccctggccgt
     9301 cgcccttccg gtcacggtcg tgtcgtacct ggtcagccaa ctcgccctgg gcccccacgg
     9361 gtcgacgctc gacgcggacg gcgtcccgcg cgccctggcc ggcgcggtcg tctacctgac
     9421 cctcatgagc ctgctcgccg taggggtggc ggccgccgcc cgcagtgccg tcctcccgct
     9481 ggccgtgctg ctgccgctgg tgctggtcgg ctcgcagatc ctgtccgtca tcggggcgac
     9541 caaggaggtg gcgcgctggt tccccgaccg ggccggcgcc cagatgctca ccgtcgactc
     9601 cggcgacgcc ctcaccggcc tcgtcgtgct gctcgcgtgg accgcggccg ccctgacggc
     9661 cggctggctc cggcaccgtt cgtgggacgg gtgacgggac gcgcctgagg catgtgcccc
     9721 atgcgcgtgg gacatggccc ctcgtaggtt cccggacagg tcacggggcc gaaaaggtcg
     9781 gcccgggccg ttcgacccac gggaggaacc atgaccgcga tctacgagcc gcccgccctg
     9841 caggagatcg gcgacttcga cgagctcacc aagtgcctcg gcatcgggag ctgcaacgac
     9901 ttcgccggct gcggttacgc cgtcgtctgc ttctggtgat cgcaccggtg ccggtgtgcc
     9961 cctcgtggca caccggcacc gcccggggga gtgaggcgac atggaattca cagtgcttcc
    10021 ggactgtccc gccggcgccg cgctggcgga ccgggtggcg gcgccgaagc ggatcgacca
    10081 cgcgtcggga cggccctgga tcgtggggga ctggcccgag ggcgggacga cggtgaccga
    10141 ggccggcacc cgccgcctgg cggtgttcgg acacacccgg cccgacgacg cgggaacggc
    10201 ctccgcgctc ggccggatgc gctcgctgca cgacgtcgac cgggtcgcgt cccggctgcc
    10261 cggggtcttc cacctcgcgg cctccctgga cggcgcggtc cgcctccagg gctccgtggc
    10321 cggcgtacgg caggtcttca ccgcccgggt cgacggggtg acggtcgccg cgagcgccgt
    10381 ggacccgctg ctgcgcctca ccggcgcggg actcgacgag accctgctgg ccgcacggct
    10441 gctggcgccc ggcggggcgc cctggccgct ctcgccgcgc ccggtccgcc gcggcgtgga
    10501 cgcgctgccc accggccact ggctggagct cggcgccgac ggccgggccc gcagcgtccg
    10561 gtggtgggaa ctgcccgagg cgaccctctc gctggaggag ggtgccgggg ccgtccgttc
    10621 cgcgctcacc gacgccctcg cgacccgtgt ggacccgcac cgcacggtca gcgcggacct
    10681 ctccggcgga ctcgactcga ccaccctgtg cttcctcgcc gacgcggccg gcgccgacct
    10741 ggtcacctac cacgtcatgc cgctcgacga ggccaacgag gacaccgcat gggcccgcaa
    10801 ggccgccgcg catctgccgc acgcccgcca ccacatgctc gccgcggacc gcgccgccaa
    10861 cctgttcgac atcggctaca ccgccgacac cgtcaacgcg gcccccgagg gcccctcgac
    10921 gtgggcctcc ggactcgcgc acatccggga cctcgccggg cgggccaccg ccgagggggc
    10981 ggccctgcac ctgtccgggt tcggcgggga cgaactcttc ggccggatgc ccgcctgcgc
    11041 ctggtccctc gcccgcctcc gcccggccga cggcctgcgg ctggtgaacc gctaccggct
    11101 ggccaaccgc tggccctggc gcagcaccct gcggcagctc gcggaccgtt cgacgttcgc
    11161 gcagaacctg accgaggtcg ccgggcggat caccgccccg cccccgccgg tcaacgagcc
    11221 cgacttcggc tgggtgttcg cgccgcgcat gcccgcctgg gccaccccgg acgcggtggc
    11281 cgccgtgcgg gacctgctga ccacggcagc cgaacggacc ccggaaccgc tggacgccga
    11341 ccgggcccgg caccaggcgc tgtcctccat cgtgttcgag gggaacaccg tccggcaggt
    11401 caacaccgcc gtcgccggca ccggcctggt ctgggaggcg ccgttcctcg acgaccgggt
    11461 tctcgaggcg gccttcgcca cccggatcga cgagcggctg gccgccggac ggttcaagcc
    11521 gctgctgacc accgccgtgc ggggcctcgt gcccgacgac ttcctcgccc gtcgcgacaa
    11581 gggagagttc agcgccgaga cgttccgggg catcgaacgc aaccgggacc ggatcctgga
    11641 cctctgcgag gactcgctcc tggcccggct cggcctcgtc gacccgcacg ccttccggtc
    11701 cgcggtgctc aaccccgggc cgatgtccca tcacctccag ccgatccaga ccacggtggc
    11761 gtgcgagagc tggctgcggg cgcaccccca ggacaccgga gagaaccgat gaaactgagt
    11821 cttgcccgcg acgtcaccct cacgcccgtc gattccgggg cggtcctgct cgacggccgc
    11881 cgcggccgct actaccagct gaacgcctcc ggctccgcga tcctgcacaa gctgctcgac
    11941 ggcgacactc ccgccgcggc cgccgcgagc ctgtcggagt ccgcccccgt cagcgaggag
    12001 cgggtgcacc aggacgtgct ggccctggtc cgctcgctga gcgaggccga cctcgtggag
    12061 gtgacacagt gaccacgccc gccgtcgccg aacaggccac gcgcctcccg ctgcaccggc
    12121 agatcgcccc cagatgcgcg gccggcgccg cccgtctgct ggtcaggctg cccccggccc
    12181 ggctgcaccg ggtgctgcgc gtcctgagca agggttcccg gcccgccgga tacgcccagg
    12241 tggcgcgggc ccggcggtcc gtcgtctcgg tcagcacccg ctgcgcgggc ctcggctgcc
    12301 tccagcggtc cgtcgccacc gtgctgctgc tccgcgtccg gggcaggtgg gccgactggt
    12361 gcaccggctt ccgggtgcag cccttcgccg cgcacgcctg ggtcgaggcc ggcggccgcc
    12421 cggtcgacga gcccggagag gtcggcgtgt tccgcaccgt gctggccgtg cgccgcaccg
    12481 gaggcggctc atgacggcga tccgggccga gggcctctac gcgtactacg gcaccacgcc
    12541 ggccgtgaac ggcctccacc tgaacgtccc cgagggggcc accttcgggt tcctggggcc
    12601 gaacggcgcc ggcaagacca ccaccatcag catgctcacc acgctgctca ggcccacggc
    12661 gggccgcgcg gaggtggccg ggttcgacgt caccacgcac gccgccgagg tgcggcgcag
    12721 gatcggcatc gtcttccagg agtcgacgct cgacctggaa ctcacggcga ccgagaacct
    12781 gcgattccag gccgacctgt gcggtctggg ccgggccgag gcgcgcgccg cggtggccgc
    12841 gatgctcgac atgatggagc tgacgggacg cgacaggacc cccgtgcggc acttctccac
    12901 cggcctgcgc cgacgcctgg agatcgcccg cggcctgctc ggctccccgc gcgtgctctt
    12961 cctcgacgag ccgaccaccg gcctcgacac gcagacccgt gcggccgtct ggcaccacct
    13021 ggaccggctc cgcgaggagc agggcatcac ggtcttcttc accacgcacc agctggagga
    13081 ggccgagcac tgcgaccgca tcgcgatctt cgaccgcggc aagctggtca cggagggctc
    13141 acccgcggag ctgaagtccg tcatcggggc cgacgtcgtc gacctgcgca ccgatgacga
    13201 ccggctggcc gtggacctgc tgtccgaccg cttcggcctg acggccgaga acacccccgg
    13261 cggactgcgg ctgagggtgc aggacggcgc ctccatggtg ccccgcctgt gcaccgggct
    13321 gggcctgggc gtgcggtcgg tgaccgtcac cccgccctcg ctcgacgacg tctacctgca
    13381 ccacaccggg accgcgatca gggacagcgg gtccgacgcc cgctcgctcg acagcctcgg
    13441 ggagggactg cgatgacccg gaccgacatc ccgccgagcg tcaccgtgac gaaaccggcc
    13501 ccgccctcgt ccgaggggcg gacctggcac accgtacggc cctacgcgct gctgtggcgg
    13561 cgtgagatga cccggctgcg gcacaatccg ctgcgcctcg tcatgggtct ggtcaccccg
    13621 ctgctgttcc tcgtcgtgct gggcacgggg ctggaagcgg cgtcgtccac gctcggcaag
    13681 gcccagctga acgactaccg ggcctacctg ttcccgggcg cgctcgtgat gtccgtgcag
    13741 gccccggcca tcgccgtggg catctcgctc gtctgggacc gccggctcgg catgctgcgc
    13801 cagatgctcg tctccccgtt ccctcggtcg agcatcgtcc tcggcctcgc gctcggcgga
    13861 gccaccacgg gcgccgtcta cggcctcgcc ctgctcgcgg tcggaggcat cgcgggcgtc
    13921 cggtacacgc cgatgctgct ggtcgtcctc gtcgaactgc tcctggtctc cctgctcttc
    13981 acctcgttcg gccttctcgc cgcggtcacc atccggcagg tcgacacctt ccagatcgtg
    14041 gtgaacctca gcctcatgcc gctgatgttc ttctccggcg cgatgttccc gcccaacggg
    14101 ctgccgggct ggctcgacac cgtcgtcaag ctgaacccgc tgacctacgg cgtcgacgcc
    14161 gtgcgccgga cactgcccgg ccccgacgtc ctcacctcgg agcagacccg gctgatgctc
    14221 ggcgactgga acccgcccgt gtacgcggaa ctcggcgtgc tggccgccct caccgccgcc
    14281 gtactgggcc tcgccaccta ccggttctcc cgggcgcagt gaaagggggc cggccgtgac
    14341 gttcgtgcgg acatgggcgg gcaccgcggc caggatcgtc ctcgcggcgg tcctgggcta
    14401 cgccggctgg gtgaaggtcc aggacctcac gggggccggc cgtaccgtcg ccctctacca
    14461 gctcgtcccc gaggaaagcg cacaactcgt cggcgccgcc gtggcgttcg tcgagctggc
    14521 gcttgccctg ctgcttctcg ccgggctcgc cacccgcgcc gtggcggccg tcaccgccct
    14581 gctgatggtc acgtacatcg cggccatcgc ctcggtgtgg gcgcgcggga tgtccatcga
    14641 ctgcggctgc ttcagcagcg gcggcaccct caccggcggc gcggaacggg gctacgtcgt
    14701 cgacatcgcg cgcgacctcg ccttcctcgg cgccgccgcg ttcctgatca cccgcccgcg
    14761 gacccgttac gccctggacc gctgggtcct ggaaacgaag gagcgataga catgcagccg
    14821 tcgacggacg cccaggtccg ggaaatggtc caccggcgcc ggcgacgccg ccgcactgtg
    14881 ctggtgtcgc tcgcggcggt ggtcacggtg ctcggcgccg cgctcgtcgg cgccggcctg
    14941 gtccgggcca ccgacaccga gcccggcgat gcgcccgagg aggtgcccgc cggtgtggcc
    15001 gccgaccggg ccggactcgt cacgtccgag ggtccggtcc gtgtcgacct ctacctcgac
    15061 tacctctgcc ccgagtgccg catcaccgag aaggccctcg cgcccgaact gagggagatg
    15121 caggaacgcg gagaggtgcg cgtcgtccac cacccggtcg ccttcctcga cgaccgcagc
    15181 gcccccgccg gctactccac ccgggccgcg tccgccgccg cctgcgccgc tgaccggaac
    15241 aagttcgagc cctacacggc ggcgctcttc gacgagcagc cgccggagca gggccccggc
    15301 ctcgacaccg accgcctggt cgccctcggc cgggacgtcg gcatcaccgg cgcgtccttc
    15361 gagcggtgcg tccgcgacgg cacgtaccgg ccctgggtga cgtacgtgtc cgacgtcgcc
    15421 gcctcccgcg gggtggccct caccccgacc gtgaaggtgg ccggcaagcg cgtggacctc
    15481 tccggacccg accgggcgaa ggcgttcgtg cgagcggtgg aggagagccg ttcatgagcc
    15541 gcctcaccgt ggcgctgacc ggggactgca tgatgacgcg gggcgcgccc gtcacctccg
    15601 accccgccgc cggccgactg ggcgaggtgc tgcacacggc cgacttcgcc ttcaccaacc
    15661 tggaggtcgc gcccgccacc ggacgcggcc accccgtacc cgacgcggcc agcggaggcg
    15721 gcctgatcgc ggatcccgcc gtcctcgacg acgtgacggc gatgggcttc tccgccctga
    15781 gctgcgccaa caaccatgcc ctcgacctgg gcacggaggg cgtcctcggc acggtggacc
    15841 tgctgcgcgc caagggggtg ccgttcgcgg ggatcggcgc cgacctcacc gcggcccggc
    15901 gcccggtcta cgtggaccgg ccggccggca gcctggcgct gatcgcgtgc accgccacct
    15961 tccgggacgg gcaggaggcg gccgccccgt cccccgacct gccgggccgg ccgggactca
    16021 gcccgctgcg gcacggcgtg acgctccggg tgacggccgc gcagctggag gcgctgcggg
    16081 agatcgacgg cgccaccggg ctgcggtccc ggcgggccga ggcgcgcacc ctgctcgggt
    16141 tcgaccccgc catgcccgga ccgggccggc tcgcgctgtt cggcacccgg ttcgagaccg
    16201 cggacacccc gggcctgacc accgcctgcg acccccggga cgtccacgac atcacccgct
    16261 gggtggccga ggcgcgccgc cgcgcggacc tggtcctggt cagcgtccac tcccacgaac
    16321 ccggcccgac gcccgagacc cccgccgagt tcctgcggga cttcgcccgt gcggtgatcg
    16381 acgccggcgc cgacgccgtg gtcgggcacg gcccgcacat gctgcgcggt gtggagctcc
    16441 accggggccg gcccgtcttc cacagcctgg ggaacatcgt cagccagatc gaactggccg
    16501 accgcatccc cgccgaggac tacgcgcggg ccggcacgga cgagtccctg acccccggcg
    16561 ggtacttcga gcggatcacc gcccacggcc ggcggatgtt cgccccgcac cgccggtact
    16621 ggcagtcgct cgtgccggtg ctcaccttcg acgacggggt cttcaccggc gcccggctgc
    16681 acccggttga cctgggcttc gggcagcccg tgcaccggcg gggacggccc cggctggccg
    16741 gtccggtcga cgccaaggag atcctcaccg acttcgcgcg gctcgcggag ccccacggcg
    16801 tcaccgtcac cgtcggcgac gacgggacgg gggaactggt ggcggcggaa acctgacggc
    16861 ggccgccggc ccggccgata aggtgcgggg gatacaggct gacgaggcgt cggaccgccg
    16921 gacacggcgg cgacccgagg tacgagggat gcggcatggc cgaggcggtc cgggtcctga
    16981 tcgcggacga ccaggcgctg ctgcgcggga gcttccgggt cctcgtcgac tcgactcccg
    17041 gcatggagac ggtgggggag gcgggggacg gcgccgaggc cgtcgcgctc gcccgcgaac
    17101 tgacgccgga cgtggtcctg atggacctgc ggatgcccgt catggacgga atcgccgcca
    17161 cccgcgagat ctgctcggac gcgtccatgg cgggcgtgcg ggtgctggcc ctcacgatgt
    17221 tcgacatgga cgagtacgtc tacccggccc tccaggcggg cgcgagcggc ttcatgctga
    17281 aggacgcctc gccggacgac ctcgtcgcgg gcatccgtgt catcgccacc ggcgagggcg
    17341 tgctggcccc ctcggtcacg cgtcgcgtca tcgcgaactt ctcccaccgg gacacggccg
    17401 cccggccggc cccccggctc gtcgggctca cccggcgcga acaggaggtg ctcgtgctga
    17461 tcgccaaggg cctgtcgaac gcggagatcg ccgaccggct ggtgatcagc ctgcccaccg
    17521 tgaagaccca cgtgagcagc ctgctctcga agctgaacgc ccgcgaccgc gcccagctgg
    17581 tcatcgtcgc ctacgagagc ggcatcgcgg agcgcgggat gccgccggcc tgacccgggt
    17641 ggcgacgggt cacggcagag aagatcgtcg tagggacgcg tccgtcgctc gtccggccgg
    17701 gccgcgttcc gcgcggagga gcgcgcgggc cgcccggagg acgtcctcgt cctctcccgc
    17761 accgcaggcg acgagcacgc ggagcagtcg ggtcccccac acgtccgggt ccgtggcgtg
    17821 ggcgtcgagg acacggacgc cgtaggttcc tcccgtgccg tcgggggcgg tcaggcgtcg
    17881 gcggaccgcc tgcctcagcg cgtcgtgcag gtcgtggacg gttccgcggg ccgtgcccgt
    17941 cagtccggcc gccgctccgg tggtcagcgc ggtggccacc agttccgctc cggtcaccat
    18001 gggtcccttc ggatgtgcgg gttgtgcgcg ttcgcaccgg tcactgtgtc cgcaggtgct
    18061 gcaatgctga tgaactggcg atgaagtgcg ggagcggagg ctcgggttgg gcggggaatc
    18121 tcagcggctg cgtatcgagg tgctcggccc gttgcgggcg tggcgggacg gaggccctct
    18181 ggcgctgggg cccgtcagac ggcagaccgt gctggccgcc ctgctgctcc gggacggcgc
    18241 ggtggtgagc cacgagcaac tgctcgacgg cgtctgggga gccgacccgc ccgcgtccgg
    18301 caccaaggtg ctggccagtc acgtcaatca gctgcgcagg acgctcgacc gggcggggac
    18361 ccgtcatacg gagtcggtga tccgcagcgg gaaggggtgg taccgcttcg tcctggacga
    18421 cgtccgcctc gatctcgcgg acctcgccga gctgggcgac cgcgggctgc ggacgaaggc
    18481 gtccggtgat ctcgggggcg ccgccgagca gttgaccgag gccctcgcgc tgttccaggg
    18541 cgaaccgctg gcccagctct ccgggccgtt cgccgcggcc gagcgggagc gcctctcgga
    18601 gcggcggcgg accctgcggc tggcccggct ctcctgtctg gtcgacctcg ggcggtacgg
    18661 cgacgccctg gacgacctgg cccctcttcc cgaggccgac ccgtacgacg agtcggtgct
    18721 ggcgctgcgc atgcgcgccc tctacggctg cgaacgccag gcggaggccc tccacgccta
    18781 cgaggacatg cgccggcgcc tcgccgacga gctgggcgtc gaccccggtg aggaactgcg
    18841 ccggatccac gaggccgtgc tgcgtcagga cgacgccgcg ctcctcgggg ccacggagcg
    18901 ccaggtcgtc accgtgcagc cgcctgcccg ccggcacctc gtcaaccaac tgccgggcga
    18961 caccgggcaa ctggtcggga gggaggacga gctgtccgtc ctgaccgccc cgggccccga
    19021 cgacgcggtc tccgtcgtcg ccgtggacgg tcccgccggg gtcggcaaga cggccctcgt
    19081 cgtgcgggcg gcccaccgca tccgcgaccg ctaccccgac gggtgcctct tcgtggacct
    19141 gcacgcccac agccgcgaac gcggcaggcc cgcaccggaa agcgtgctgc gccggctgct
    19201 gcgcgccctc ggggcggacg gcagcgaggt gccggacgac ctcgacgacc tcatgaccgc
    19261 ctggcggacc gtcaccagcc cgctgcgact gctgctcgtc ctcgacgacg ccgccggcac
    19321 ccgtcagatc cgcccgctgc tccccgcggg gcccggcagc agggtgatcg tcaccggccg
    19381 gcagcggctc gcgggcctgg acgccgaccg ccgggccacc ctggagactc tcggccccgg
    19441 cgaggcggcc tcgctgctgc ggcagatcgt cggccgggag cgcacggacc gggaacccga
    19501 ggcgacaagg gacctggtcc ggctgtgcgc cggactgccg ctggcgctgc gcatcgtggg
    19561 ttcgcgcctg cagacccggc ccgcctggac gctggcgtac ctggtggagc ggatggcgga
    19621 cgacgagggg cgtctgggcg aactcagcac gggtgaccgc agcgtggagg ccgccttccg
    19681 gctgtcgtac gaccagctgc cgccggagca gcggcggggt ttcaggacgc tggggctggc
    19741 gccgacggtg gagttcgacg tgcgcacccc cgcggccatg ctccgccggc ccgcccgtga
    19801 cgccgagcgg atcctggaga gcctggtcga caccagcctg ttgcagcagc cgcggccggg
    19861 ccgttaccgg atgcacgacc tggtgcgggt ccacgcgcgc cggctggccg aggccgcgca
    19921 cgccgaggca ggaccggcgc gggaggcggc gctgcgcctc tacctggacg cggcccgcgt
    19981 ggccagcgac tggggcgccg gcggctatcc caccggtccc gggcggtcag gggcgtcgtt
    20041 ctccggctgg cgggaggccg cggtgtggct ggacgaggcc ggcggggaac tggtggacgt
    20101 cgtcggccac gcggtggcgt ccggcgaggt ggaccacgcc tgctggatcg ccgaggccct
    20161 gacggaccac ttcgtcggcc gtggccgcct gcacgagtgc caggcggccc tcgagaccgc
    20221 gctgccgtac gtcgacgccg cggcggaccg gcgcatggcg cccgccctgc acaactgcct
    20281 cggctacacg ctcacccacc gggcgcgctt ccccgacgcc tgccgtcatc tgcggaccgc
    20341 gctggacctg agccgtgagc gcggggagcg gcacgaggaa gggcgggccc tcgtcgggct
    20401 gggggcgatg accgggctgg gggccgccgg accggacgcc gacgaggtcg aggagggggt
    20461 gtccgtgctc gtcgcgggga gggagctcgc cgaggggtac gacgaccact ggctcgtcgc
    20521 gatggcctcc ttcgccctcg ggttcgtccg ccaccgccag ggccgggacg aggaggcgct
    20581 ggcctgcttc cgtgatgccc acacccgcgg ccgggccatc ggacggccgc gtgccctgag
    20641 cagggcgctg acctccaatc tcgatctgca cctctatctc gacccgtcgg cggccagggt
    20701 tctgttccgt cgggtggccg acctgatccg gcggaccggc gacgtcctgc tccacaccct
    20761 cacgctggcc aggctcggca cggcggagca caaggcgggg aacctggacg cggcggccga
    20821 ggtgttccgc cgtgcccttg cccagcacgg gacgccgggc ccgctcgacg atcccgacca
    20881 cgaccggcgg gagatggacc tgcgctgccg gctgggccgc acctaccggg cggcggggcg
    20941 gcccgccgag gcccgggagc agttccgcgt cgccctggcc gtgccgggcg ccgacaggta
    21001 cccgaaggag cacaagcagg cggtcgaagg gctcgccgcc acggaggtgc cgtcgcgggc
    21061 cggctgacgg gtacggcggc cgaaaagaac gaggccccgg tcgttcacct ctggcgaccg
    21121 ggtcctcgtg aagcggatcg gccggcggcg acactccccc gagttgcgcg tgacgcgcgc
    21181 acccccgggc cggatccgat gtagtgatca catcaaacct tgccaacgga tggctaacat
    21241 gaggccaatg gattccccat gggtcacccg gaaggcaggg gtggggcgtg acacggcgcg
    21301 gcctgcgctt cggactgctg gggacgcctg tcctctacgg cggtgagccg ccccccggcg
    21361 agccctcctc cgacggggcg ggtcccggtg acgcggtacc ccctcacgcc ccctgcacac
    21421 gcggcggcgc ccccgtcctc cccgtcacca gccccaaact ccgcaccctc ctcgccgccc
    21481 tgctgctcga cgccggacgg gtcgtctccg tcgaagtgct gaaggacgcc ctgtggggcg
    21541 gggggccgcc cgtctccgcg ctgccctcgc tgcacaacca cgtcgcccgg ctgcgccgcc
    21601 tcctcgacga gcccggccgg ctcctcaccg tgcctacggg gtacgtcctc cgcgtcgacg
    21661 acggcgaact cgacgtgtgg gtcttcgacg cccacgtctc ggccgcccgt gccgcccgcg
    21721 ccgagggcga ctgggagcgg gtgcgcggcg agtgccgggc cgcgctcggg ctgtggcggg
    21781 ggagcccgct gggcgggctg gcccccgagg tgggcgggta cgccctcgtg cgccgcctgg
    21841 aggaggcgcg gctgctgctc cacgagtggc gctacgacgc cgaactcgcc ctgggcgacg
    21901 acggcctggc cgacctcgtg cccgagctgt cagccctggc cgccgagcat ccgctgcggg
    21961 aggcctacca ccgtcagctc atgctcgccc tgcaccgcac cggccgtcag gccgaggccc
    22021 tcgtcgtcca ccgtgatctg cgcgcccgcc tcgccgacga actcggtgtg gagccgggga
    22081 agcccgtgcg tgaggcgcac gtcgaggtgc tgcgggggag cggtacgcgg gacggcgcgt
    22141 ccagatgggt gcggcccgcg caactgccgc ccgcgcccgc ccatttcgtc gggcgggcgg
    22201 atgtccggga gggaatcgcc cgggcgctct ccgggacggt cgggggcggg gttgctgtgg
    22261 tcagcggcac ggccggcgtc gggaagagcg ccctcgctct ccgggtcgcg cataccctgg
    22321 aggaacgttt cagcggcggc cagctccacc tcaacctgcg tgccacggaa cccggttcgg
    22381 cgccgctgac agtcgaggag gccctcgcct cgctcctccg cgatctcggc gtccgctccg
    22441 gcgacgtccc cgggcatccc gacgccgcct ccgcgttgct gcggtccctg ctcg
//
