LOCUS       NZ_BMSZ01000041         4528 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces badius strain JCM 4350 sequence41, whole genome shotgun
            sequence.
ACCESSION   NZ_BMSZ01000041
VERSION     NZ_BMSZ01000041.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces badius
  ORGANISM  Streptomyces badius
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 4528)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 154X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649415.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:57:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,894
            CDSs (total)                      :: 6,825
            Genes (coding)                    :: 6,582
            CDSs (with protein)               :: 6,582
            Genes (RNA)                       :: 69
            rRNAs                             :: 2 (23S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 70 of 244
            Pseudo Genes (incomplete)         :: 206 of 244
            Pseudo Genes (internal stop)      :: 7 of 244
            Pseudo Genes (multiple problems)  :: 38 of 244
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:11:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4528
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSZ01000041.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..>664
                     /locus_tag="IE135_RS33335"
     source          1..4528
                     /culture_collection="JCM:4350"
                     /db_xref="taxon:1941"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces badius"
                     /strain="JCM 4350"
                     /submitter_seqid="sequence41"
                     /type_material="type strain of Streptomyces badius"
     region          1..4528
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="NRPS"
                     /region_number="1"
                     /rules="cds(Condensation and (AMP-binding or A-OX) and not
                     alpha_am_amid)"
                     /tool="antismash"
     cand_cluster    1..4528
                     /SMILES="NC(CCCCN)C(=O)O"
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(Condensation and (AMP-binding or
                     A-OX) and not alpha_am_amid)"
                     /kind="single"
                     /product="NRPS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..4528
                     /aStool="rule-based-clusters"
                     /category="NRPS"
                     /contig_edge="True"
                     /core_location="[705:>3079](+)"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      706..>3079
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(Condensation and (AMP-binding or A-OX)
                     and not alpha_am_amid)"
                     /neighbourhood="20000"
                     /product="NRPS"
                     /protocluster_number="1"
     CDS             5..>664
                     /NRPS_PKS="Domain: PCP (107-171). E-value: 3.2e-29. Score:
                     92.9. Matches aSDomain: nrpspksdomains_IE135_RS33335_PCP.1"
                     /codon_start=3
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028843.1"
                     /locus_tag="IE135_RS33335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="phosphopantetheine-binding protein"
                     /protein_id="WP_199889929.1"
                     /sec_met_domain="PP-binding (E-value: 4.4e-18, bitscore:
                     53.4, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIELGEIEAALLSHTALTQAAVLVREDVPGDKRLVAYVVPATPG
                     DTVDTSALRAHVGRSLPEYMVPSATVVLDALPLTVNGKLDRRALPAPEYTAASSGRAPA
                     NAQEEILCSVFAEVLGVSGVGVDDNFFELGGHSLLAVRLVERLRARGVPVDVRALFAAP
                     TVAGLATASLERETVTVPENVIPADAEALTPEMLPLVDLTAEEIERIAARVQGGAAN"
     CDS_motif       148..165
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33335_0001"
                     /evalue="9.90E+01"
                     /label="NRPS-A_a10"
                     /locus_tag="IE135_RS33335"
                     /protein_end="53"
                     /protein_start="47"
                     /score="-2.9"
                     /tool="antismash"
                     /translation="VDTSAL"
     CDS_motif       250..276
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33335_0002"
                     /evalue="8.50E-03"
                     /label="NRPS-A_a10"
                     /locus_tag="IE135_RS33335"
                     /protein_end="90"
                     /protein_start="81"
                     /score="8.5"
                     /tool="antismash"
                     /translation="GKLDRRALP"
     aSDomain        328..519
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE135_RS33335_PCP.1"
                     /evalue="3.20E-29"
                     /label="IE135_RS33335_PCP.1"
                     /locus_tag="IE135_RS33335"
                     /protein_end="171"
                     /protein_start="107"
                     /score="92.9"
                     /tool="antismash"
                     /translation="EEILCSVFAEVLGVSGVGVDDNFFELGGHSLLAVRLVERLRARGV
                     PVDVRALFAAPTVAGLATA"
     CDS_motif       391..420
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33335_0003"
                     /evalue="1.10E-03"
                     /label="PCP_mC"
                     /locus_tag="IE135_RS33335"
                     /protein_end="138"
                     /protein_start="128"
                     /score="12.2"
                     /tool="antismash"
                     /translation="NFFELGGHSL"
     assembly_gap    665..705
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     CDS_motif       706..804
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33340_0001"
                     /evalue="1.90E-15"
                     /label="C2_dual_024-063"
                     /locus_tag="IE135_RS33340"
                     /protein_end="33"
                     /protein_start="0"
                     /score="49.4"
                     /tool="antismash"
                     /translation="MLRFDARGRLDRFVDALQKVVDRHDILRTGFAW"
     gene            <706..>3079
                     /locus_tag="IE135_RS33340"
                     /old_locus_tag="GCM10010253_67540"
     CDS             706..>3079
                     /NRPS_PKS="Domain: Condensation_Dual (1-261). E-value:
                     4.2e-102. Score: 333.4. Matches aSDomain:
                     nrpspksdomains_IE135_RS33340_Condensation_Dual.1"
                     /NRPS_PKS="Domain: AMP-binding (429-784). E-value: 2.3e-93.
                     Score: 304.9. Matches aSDomain:
                     nrpspksdomains_IE135_RS33340_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     Condensation"
                     /gene_functions="biosynthetic (rule-based-clusters) NRPS:
                     AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IE135_RS33340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_67540"
                     /product="amino acid adenylation domain-containing protein"
                     /protein_id="WP_199889930.1"
                     /sec_met_domain="Condensation (E-value: 4.8e-42, bitscore:
                     131.7, seeds: 42, tool: rule-based-clusters)"
                     /sec_met_domain="AMP-binding (E-value: 1.8e-94, bitscore:
                     304.2, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRFDARGRLDRFVDALQKVVDRHDILRTGFAWEGLREPVQVVAR
                     AAAVPVDEIRLDVRADENPVEQLLAACAASMDVGRAPLLRAYVATEPGSDRWLMVLQNH
                     HLVLDHTTFDVLLDEVRALLRGDAEGLAVPVPFREFVAQARLGVSRAEHERFFGELLDG
                     VTEPTAPYGLLDLRKGSGGITDVSTTLDAGLAARLREQARRLGVSPATLFHLVWARVVA
                     VTSGRDDVVFGSVLFGRMQAGTGADRTPGLFINTLPVRVPTGHVSVGEAVRGMQRQLAD
                     LLVHEHAPLTLAQQAADLAAETPLFTSLLNYRHDMAGSGGPGAATSGALEGVELLSAHE
                     RTNYPLAVSIDDAGTDFGVTVQAADPIAPHDVCTLIHTVTAELVRVLEAESATELCRIE
                     ALGESERHHVLTAWNDTARGTRPTTLTELWHAQVARTPEAVAFVHESVRLTYAELDARA
                     DRWAEVLVDRGVGPEQLVAIALPRSADTVVALLAVLKAGGAYLPLDPSHPAERTAYVLK
                     DARPTALITARASAEGFANPAVPRLLIEDMERAEPSAGRPPRAREVLPSHPAYVIYTSG
                     STGRPKGVVVSHGSAAAFSTGIAAAYGIGEQDRVLGFAAFTFDVSVFEVFTTLTAGATL
                     ILAGDEDRLNADRLHGLMAEQEVTVAELPPALMSLLRPDGLPALRLVSTGGELPAGRLV
                     DQWARPEREFWNGYGPTETTVAITLMHCLPPSHGQAPPIGRPMPNARVFVLDAGLCPVP
                     AGVAGELYLAGDQLARGYLGRPGLTAERFVANPFGGPG"
     aSDomain        709..1488
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE135_RS33340_Condensation_Dual.
                     1"
                     /domain_subtypes="Condensation_Dual"
                     /evalue="4.20E-102"
                     /label="IE135_RS33340_Condensation_Dual.1"
                     /locus_tag="IE135_RS33340"
                     /protein_end="261"
                     /protein_start="1"
                     /score="333.4"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHLVLDH"
                     /tool="antismash"
                     /translation="LRFDARGRLDRFVDALQKVVDRHDILRTGFAWEGLREPVQVVARA
                     AAVPVDEIRLDVRADENPVEQLLAACAASMDVGRAPLLRAYVATEPGSDRWLMVLQNHH
                     LVLDHTTFDVLLDEVRALLRGDAEGLAVPVPFREFVAQARLGVSRAEHERFFGELLDGV
                     TEPTAPYGLLDLRKGSGGITDVSTTLDAGLAARLREQARRLGVSPATLFHLVWARVVAV
                     TSGRDDVVFGSVLFGRMQAGTGADRTPGLFINTLPVRV"
     aSModule        709..3442
                     /complete
                     /domains="nrpspksdomains_IE135_RS33340_Condensation_Dual.1"
                     /domains="nrpspksdomains_IE135_RS33340_AMP-binding.1"
                     /domains="nrpspksdomains_IE135_RS33345_PCP.1"
                     /locus_tags="IE135_RS33340"
                     /locus_tags="IE135_RS33345"
                     /monomer_pairings="Lys -> Lys"
                     /monomer_pairings="Lys -> Lys"
                     /tool="antismash"
                     /type="nrps"
     CDS_motif       835..861
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33340_0002"
                     /evalue="1.00E+01"
                     /label="NRPS-A_a2"
                     /locus_tag="IE135_RS33340"
                     /protein_end="52"
                     /protein_start="43"
                     /score="0.6"
                     /tool="antismash"
                     /translation="ARAAAVPVD"
     CDS_motif       1015..1077
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33340_0003"
                     /evalue="2.00E-07"
                     /label="C3_dual_135-156"
                     /locus_tag="IE135_RS33340"
                     /protein_end="124"
                     /protein_start="103"
                     /score="23.9"
                     /tool="antismash"
                     /translation="HHLVLDHTTFDVLLDEVRALL"
     CDS_motif       1111..1152
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33340_0004"
                     /evalue="3.20E-06"
                     /label="C4_dual_169-182"
                     /locus_tag="IE135_RS33340"
                     /protein_end="149"
                     /protein_start="135"
                     /score="19.6"
                     /tool="antismash"
                     /translation="PFREFVAQARLGVS"
     CDS_motif       1390..1485
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33340_0005"
                     /evalue="1.90E-15"
                     /label="C5_dual_260-291"
                     /locus_tag="IE135_RS33340"
                     /protein_end="260"
                     /protein_start="228"
                     /score="49.0"
                     /tool="antismash"
                     /translation="DVVFGSVLFGRMQAGTGADRTPGLFINTLPVR"
     CDS_motif       1531..1635
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33340_0006"
                     /evalue="1.40E-06"
                     /label="C67_DCL_14fromHMM"
                     /locus_tag="IE135_RS33340"
                     /protein_end="310"
                     /protein_start="275"
                     /score="20.6"
                     /tool="antismash"
                     /translation="QRQLADLLVHEHAPLTLAQQAADLAAETPLFTSLL"
     aSDomain        1993..3057
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE135_RS33340_AMP-binding.1"
                     /evalue="2.30E-93"
                     /label="IE135_RS33340_AMP-binding.1"
                     /locus_tag="IE135_RS33340"
                     /protein_end="784"
                     /protein_start="429"
                     /score="304.9"
                     /specificity="substrate consensus: Lys"
                     /tool="antismash"
                     /translation="HAQVARTPEAVAFVHESVRLTYAELDARADRWAEVLVDRGVGPEQ
                     LVAIALPRSADTVVALLAVLKAGGAYLPLDPSHPAERTAYVLKDARPTALITARASAEG
                     FANPAVPRLLIEDMERAEPSAGRPPRAREVLPSHPAYVIYTSGSTGRPKGVVVSHGSAA
                     AFSTGIAAAYGIGEQDRVLGFAAFTFDVSVFEVFTTLTAGATLILAGDEDRLNADRLHG
                     LMAEQEVTVAELPPALMSLLRPDGLPALRLVSTGGELPAGRLVDQWARPEREFWNGYGP
                     TETTVAITLMHCLPPSHGQAPPIGRPMPNARVFVLDAGLCPVPAGVAGELYLAGDQLAR
                     GYLGRPGLTAERFVA"
     CDS_motif       2176..2217
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33340_0007"
                     /evalue="4.70E-06"
                     /label="NRPS-A_a2"
                     /locus_tag="IE135_RS33340"
                     /protein_end="504"
                     /protein_start="490"
                     /score="19.6"
                     /tool="antismash"
                     /translation="LAVLKAGGAYLPLD"
     CDS_motif       2410..2469
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33340_0008"
                     /evalue="2.00E-11"
                     /label="NRPS-A_a3"
                     /locus_tag="IE135_RS33340"
                     /protein_end="588"
                     /protein_start="568"
                     /score="35.5"
                     /tool="antismash"
                     /translation="AYVIYTSGSTGRPKGVVVSH"
     CDS_motif       2935..2964
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33340_0009"
                     /evalue="3.10E+01"
                     /label="NRPS-A_a2"
                     /locus_tag="IE135_RS33340"
                     /protein_end="753"
                     /protein_start="743"
                     /score="-0.8"
                     /tool="antismash"
                     /translation="VLDAGLCPVP"
     CDS_motif       2962..3051
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33340_0010"
                     /evalue="3.40E-17"
                     /label="NRPS-A_a6"
                     /locus_tag="IE135_RS33340"
                     /protein_end="782"
                     /protein_start="752"
                     /score="54.5"
                     /tool="antismash"
                     /translation="PAGVAGELYLAGDQLARGYLGRPGLTAERF"
     assembly_gap    3080..3202
                     /estimated_length=123
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3203..>4528
                     /locus_tag="IE135_RS33345"
     CDS             3205..>4528
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: PCP (10-79). E-value: 6.1e-28. Score:
                     88.8. Matches aSDomain: nrpspksdomains_IE135_RS33345_PCP.1"
                     /NRPS_PKS="Domain: Condensation_LCL (93-394). E-value:
                     5.1e-117. Score: 382.1. Matches aSDomain:
                     nrpspksdomains_IE135_RS33345_Condensation_LCL.1"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Condensation"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1127: condensation domain-containing protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012873.5"
                     /locus_tag="IE135_RS33345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="condensation domain-containing protein"
                     /protein_id="WP_234428206.1"
                     /sec_met_domain="PP-binding (E-value: 1.7e-19, bitscore:
                     58.0, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="Condensation (E-value: 2.4e-73, bitscore:
                     234.4, seeds: 42, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVGRAPANAQEEILCSVFADILNVPHVNVDDNFFELGGHSLLAVR
                     LVSRLRSALGVEIGVRTVFEAPSVESLLKRLEDAEQARPALSAQTRPELVPLSFAQQRL
                     WFLGELEGPNATYNIPAGIRLHGTLDTQALSAALNDVVARHEVLRTVFPTIDGQPQQHI
                     LDTDQAHCELKTVHVSSPQETAEAMAQAAASVFDLSADLPLRAWLIPTGPDEHILMLVL
                     HHIAGDGWSMAPLAHDLSLAYTARTTNSTPAWQPLPVQYADYTLWQRDLLGQETDPHSV
                     LARQLAYWRHTLADLPDELALPLDRPRPATATHHGGIVDLTVEPDTHTRLLHTARTHGV
                     TLFMVLQAAMAALLSRLGAGRDIPLGVPVAGRSDEALENLIGFFVNTLVIRTDLTGDPT
                     FTELLERVRDTGLGAYAHADIPFERLVEELAPTRSMARHPLF"
     aSDomain        3236..3442
                     /aSDomain="PCP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE135_RS33345_PCP.1"
                     /evalue="6.10E-28"
                     /label="IE135_RS33345_PCP.1"
                     /locus_tag="IE135_RS33345"
                     /protein_end="79"
                     /protein_start="10"
                     /score="88.8"
                     /tool="antismash"
                     /translation="EEILCSVFADILNVPHVNVDDNFFELGGHSLLAVRLVSRLRSALG
                     VEIGVRTVFEAPSVESLLKRLEDA"
     CDS_motif       3299..3328
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33345_0001"
                     /evalue="2.50E-03"
                     /label="PCP_mC"
                     /locus_tag="IE135_RS33345"
                     /protein_end="41"
                     /protein_start="31"
                     /score="11.1"
                     /tool="antismash"
                     /translation="NFFELGGHSL"
     aSDomain        3485..4387
                     /aSDomain="Condensation"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE135_RS33345_Condensation_LCL.1
                     "
                     /domain_subtypes="Condensation_LCL"
                     /evalue="5.10E-117"
                     /label="IE135_RS33345_Condensation_LCL.1"
                     /locus_tag="IE135_RS33345"
                     /protein_end="394"
                     /protein_start="93"
                     /score="382.1"
                     /specificity="C activity: active"
                     /specificity="Active site motif: HHIAGDG"
                     /tool="antismash"
                     /translation="LVPLSFAQQRLWFLGELEGPNATYNIPAGIRLHGTLDTQALSAAL
                     NDVVARHEVLRTVFPTIDGQPQQHILDTDQAHCELKTVHVSSPQETAEAMAQAAASVFD
                     LSADLPLRAWLIPTGPDEHILMLVLHHIAGDGWSMAPLAHDLSLAYTARTTNSTPAWQP
                     LPVQYADYTLWQRDLLGQETDPHSVLARQLAYWRHTLADLPDELALPLDRPRPATATHH
                     GGIVDLTVEPDTHTRLLHTARTHGVTLFMVLQAAMAALLSRLGAGRDIPLGVPVAGRSD
                     EALENLIGFFVNTLVIRTDL"
     CDS_motif       3497..3526
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33345_0002"
                     /evalue="1.00E-03"
                     /label="C1_LCL_004-017"
                     /locus_tag="IE135_RS33345"
                     /protein_end="107"
                     /protein_start="97"
                     /score="11.5"
                     /tool="antismash"
                     /translation="SFAQQRLWFL"
     CDS_motif       3548..3664
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33345_0003"
                     /evalue="6.30E-20"
                     /label="C2_LCL_024-062"
                     /locus_tag="IE135_RS33345"
                     /protein_end="153"
                     /protein_start="114"
                     /score="63.6"
                     /tool="antismash"
                     /translation="ATYNIPAGIRLHGTLDTQALSAALNDVVARHEVLRTVFP"
     CDS_motif       3869..3934
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33345_0004"
                     /evalue="1.30E-09"
                     /label="C3_LCL_132-143"
                     /locus_tag="IE135_RS33345"
                     /protein_end="243"
                     /protein_start="221"
                     /score="30.5"
                     /tool="antismash"
                     /translation="LHHIAGDGWSMAPLAHDLSLAY"
     CDS_motif       3983..4021
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33345_0005"
                     /evalue="1.30E-05"
                     /label="C4_LCL_164-176"
                     /locus_tag="IE135_RS33345"
                     /protein_end="272"
                     /protein_start="259"
                     /score="17.7"
                     /tool="antismash"
                     /translation="QYADYTLWQRDLL"
     CDS_motif       4289..4378
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33345_0006"
                     /evalue="1.60E-15"
                     /label="C5_LCL_267-296"
                     /locus_tag="IE135_RS33345"
                     /protein_end="391"
                     /protein_start="361"
                     /score="49.4"
                     /tool="antismash"
                     /translation="DIPLGVPVAGRSDEALENLIGFFVNTLVIR"
     CDS_motif       4427..>4528
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS33345_0007"
                     /evalue="5.00E-17"
                     /label="C67_LCL_14fromHMM"
                     /locus_tag="IE135_RS33345"
                     /protein_end="441"
                     /protein_start="407"
                     /score="54.2"
                     /tool="antismash"
                     /translation="RDTGLGAYAHADIPFERLVEELAPTRSMARHPLF"
ORIGIN
        1 ggttccgcat cgaactcggc gaaatcgaag ccgcgttgct ctcacacacg gccctcacgc
       61 aggccgcggt actcgtccgt gaagacgtcc ccggggacaa gcgcctggtc gcctacgtcg
      121 tcccggccac ccccggcgac acggtggata cgtcggcgct gcgggcccac gtgggccggt
      181 cactgccgga gtacatggtg ccgtccgcga cggtcgtcct ggacgccctg cccctcacgg
      241 tcaacggcaa gctcgaccgc cgtgccctgc ccgcgcccga atacacggcc gccagctccg
      301 gccgggcgcc cgccaacgcc caggaggaaa tcctgtgctc ggtattcgcg gaggtcctcg
      361 gcgtttccgg cgtgggtgtc gacgacaact tcttcgaact gggcggccac tccctcctcg
      421 ccgtccgcct ggtggagcgg ttgagggcac ggggtgtgcc ggtcgatgtg cgggcgctct
      481 tcgccgcgcc gacggtggcc gggctggcta cggcctccct ggagcgggaa accgtcacgg
      541 tgccggagaa tgtgattccg gcggacgccg aggcgctgac cccggagatg ctgccgctgg
      601 tggacctgac ggcggaggag atcgagcgga tcgcggcccg ggtgcagggc ggcgcggcga
      661 acatnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngtgct gcggttcgac
      721 gcccgagggc ggctcgaccg gttcgtcgac gcgctgcaga aggtggtcga ccggcacgac
      781 atcctccgta cgggcttcgc gtgggagggc ctccgcgagc cggtgcaggt cgtggcccgc
      841 gcggctgctg tccccgtcga cgagatccgc ctcgacgtgc gggccgacga gaatccggtc
      901 gagcagctcc tcgcggcgtg cgccgcctcg atggatgtcg ggcgggctcc gctgctgcgt
      961 gcgtacgtcg ccaccgaacc gggcagcgac cgttggctga tggtccttca gaaccaccac
     1021 ctcgtactcg accacaccac cttcgacgtc ctcctggacg aggttcgtgc gctgctgcgc
     1081 ggtgacgcgg aggggctcgc cgtgccggtg ccgttccggg agttcgtggc gcaggcgcgc
     1141 ctgggtgtgt cgcgcgcgga gcacgagcgg ttcttcgggg agctgctcga cggcgtgacc
     1201 gagccgacag cgccgtacgg tctcctcgac ctgcgcaagg gctcgggcgg tatcaccgat
     1261 gtgagcacca cgttggatgc ggggctcgcc gctcgtctgc gggagcaggc gcggcgtctg
     1321 ggcgtgagcc cggcgacgct gttccacctc gtgtgggcgc gggtcgtcgc ggtcacgtcg
     1381 ggccgtgacg acgtcgtgtt cggcagcgtg ctgttcggcc gcatgcaggc cggaacaggc
     1441 gcagaccgga caccgggatt gttcatcaac accctgcccg tacgggtacc cacggggcac
     1501 gtgtcggtgg gtgaggccgt acggggaatg cagaggcagc tcgccgacct cctcgtccac
     1561 gaacacgccc ccctcaccct cgcccagcag gccgccgacc tcgcggccga gacgccactg
     1621 ttcacctcgc tcctcaacta ccgccacgac atggccggta gcgggggccc gggcgccgcg
     1681 acgagcgggg ccctggaggg tgtcgaactc ctctcggccc acgagcgcac caactacccg
     1741 ctcgccgtgt ccatcgacga cgcgggcacg gacttcggcg tcaccgtcca ggccgccgac
     1801 ccgatcgcgc cgcacgacgt ctgcacgctg atccacaccg tcaccgccga actcgtccgc
     1861 gtactcgaag ccgagagcgc caccgaactg tgccgcatcg aggcactcgg tgagtccgaa
     1921 cgccaccacg tgctcaccgc ctggaacgac acggcacggg gaacgcgccc gaccacgctg
     1981 accgagctgt ggcacgctca ggtggccagg acgcccgagg ccgtcgcttt cgtccacgaa
     2041 agcgtgcgac tgacctacgc cgagctcgac gcgcgagcgg atcgatgggc agaggtgctc
     2101 gtcgacaggg gcgtcgggcc cgagcagctc gtggcgatcg ccctgccacg ctccgcggac
     2161 acggtggtgg cactcctcgc cgtactgaag gccgggggcg cctatctgcc cctggacccg
     2221 agccaccccg ccgagcgcac ggcctacgtg ctgaaggacg cccgccccac cgcgctgatc
     2281 acggccaggg cgtcggcgga aggcttcgcg aacccggccg tgccacgact cctcatcgag
     2341 gacatggagc gcgccgagcc gtctgccggc cgtccgcccc gggcaaggga agttctgccg
     2401 tcccaccccg cgtacgtcat ctacacctcg ggctccacgg gccgccccaa gggcgtcgtc
     2461 gtcagccacg ggtcggccgc cgcgttctcc accggcatcg ctgcggccta cgggataggc
     2521 gaacaggacc gcgtcctcgg cttcgccgcc ttcaccttcg acgtgtccgt cttcgaggtg
     2581 ttcaccaccc tgaccgccgg ggcgacgctg atcctggcgg gggacgagga ccgtctgaac
     2641 gccgaccggc tccacggcct catggccgag caggaggtga cggtcgccga gctgccgccc
     2701 gctttgatgt ctttgctgcg tcccgacgga ctgcccgcgc tgcgcttggt gtccacggga
     2761 ggcgagttgc ccgcaggtcg acttgtcgac caatgggcac gtcctgaaag ggagttctgg
     2821 aacgggtacg gaccgacgga gaccaccgtc gccatcaccc tcatgcactg tctcccgccg
     2881 tcccacggcc aggccccgcc gatcggccgc cccatgccga acgcgcgtgt cttcgtgctg
     2941 gacgcgggtc tgtgtccggt gccggcgggt gtggcgggcg agctgtatct ggcgggcgat
     3001 cagctggccc gtggatacct gggccgcccc ggcctgacgg cggagcggtt cgtcgccaac
     3061 cccttcggcg gccccggtgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnatccgtgg gccgggcacc cgccaacgcc caggaagaaa
     3241 tcctgtgctc ggtattcgcc gacatcctca acgtccccca cgtgaacgtc gacgacaact
     3301 tcttcgaact gggcggccac tccctcctcg ccgtccgcct cgtcagccgc ctgcggtcgg
     3361 ccctgggcgt ggaaatcggc gtcagaacag tgttcgaagc accgtccgta gagtccctgc
     3421 tgaaacgact cgaagacgcc gaacaagccc ggccggcctt gtccgcacag acccgccccg
     3481 aactggtacc actgtccttc gcccagcaac ggctctggtt cctcggcgag ctggaaggcc
     3541 ccaacgccac atacaacatc cccgcaggca tccgcctgca cggaacactg gacacccaag
     3601 ccctgagcgc cgcactcaac gacgtagtgg cacgccacga ggtactgcgc accgtgttcc
     3661 ccacgatcga cggccaacct caacaacaca ttctcgacac ggaccaggcc cactgcgagc
     3721 tgaaaaccgt ccatgtctcc tccccgcagg aaacagcaga ggcgatggcc caggccgccg
     3781 cgtccgtatt cgacctgtca gccgacctcc ccctgcgggc atggctgatc cccaccggcc
     3841 cggacgaaca catactgatg ctggtactcc accacatcgc cggcgacggc tggtccatgg
     3901 cacccctggc acacgatctg tccctcgcct acacagcacg caccaccaac agcacccccg
     3961 catggcaacc gctccccgtc cagtacgccg actacaccct gtggcaacgc gacctcctcg
     4021 gccaggaaac cgacccgcac agcgtcctgg cacgacagct cgcctactgg cgccacaccc
     4081 tcgccgacct ccccgacgaa ctcgcactcc ccctcgaccg cccacgaccc gccaccgcga
     4141 cacaccacgg cggcatcgtc gacctcaccg tcgaacccga cacccacacc cgcctcctcc
     4201 acaccgcccg cacccacggc gtcaccctct tcatggtcct ccaagcagcc atggcagcac
     4261 tcctcagccg actcggcgca ggccgcgaca tccccctcgg cgtccccgtc gccggccgct
     4321 cggacgaagc actcgaaaac ctcatcgggt tcttcgtgaa caccctcgtc atccgaaccg
     4381 acctgaccgg agaccccacc ttcaccgaac tcctcgaacg agtccgcgac accggactcg
     4441 gcgcatacgc acacgccgac atccccttcg aacgactcgt cgaagaactc gcccccacac
     4501 gctccatggc acgccacccc ctcttcca
//
