LOCUS       NZ_BMSZ01000028        10888 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces badius strain JCM 4350 sequence28, whole genome shotgun
            sequence.
ACCESSION   NZ_BMSZ01000028
VERSION     NZ_BMSZ01000028.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces badius
  ORGANISM  Streptomyces badius
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 59382)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 154X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649415.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:57:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,894
            CDSs (total)                      :: 6,825
            Genes (coding)                    :: 6,582
            CDSs (with protein)               :: 6,582
            Genes (RNA)                       :: 69
            rRNAs                             :: 2 (23S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 70 of 244
            Pseudo Genes (incomplete)         :: 206 of 244
            Pseudo Genes (internal stop)      :: 7 of 244
            Pseudo Genes (multiple problems)  :: 38 of 244
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:11:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 37788
            Orig. end                         :: 48676
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSZ01000028.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10888
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="2"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..10888
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10888
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:5888](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5888)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     misc_feature    431..445
                     /note="TFBS match to IolR, Regulator of the inositol
                     catabolism, confidence: medium, score: 18.26"
                     /tool="antismash"
     gene            complement(<760..1062)
                     /locus_tag="IE135_RS35365"
                     /pseudo=""
     CDS             complement(<760..1062)
                     /GO_process="GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF025063.5"
                     /locus_tag="IE135_RS35365"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="integrase core domain-containing protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MTRAPRGGLCADSVRPEPVRCSGRTRLDAFCLVARGRPAPAGAAH
                     KKIRPYRPRTSGKAERFHRTLAAEGAYQRPRTPDNERQAAFPDGPDRYDCHRPHTG"
     gene            complement(1059..2099)
                     /locus_tag="IE135_RS32045"
                     /old_locus_tag="GCM10010253_64890"
     CDS             complement(1059..2099)
                     /EC_number="1.1.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (19-269). E-value: 8.2e-13.
                     Score: 40.1. Matches aSDomain:
                     nrpspksdomains_IE135_RS32045_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1040: alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019761872.1"
                     /locus_tag="IE135_RS32045"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_64890"
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="WP_069735025.1"
                     /sec_met_domain="ADH_N (E-value: 2.5e-30, bitscore: 96.4,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.5e-24, bitscore:
                     78.3, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTVAAYAAPRAKAPLERTTIERRPVGEFDILIDIKFAGICHSDI
                     HQARDGWGQGIFPMVPGHEIAGIVAETGPGVTRFKVGDRVGVGCMVDSCGQCDACLMGR
                     EQHCAEGNTQTYNALDRGGEPTYGGYSTHLVVTEKFAVTIPEGIALDEAAPLLCAGITT
                     YSPLKRWGAGPGKKVAVVGLGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHY
                     YATSDPKTFEELAGTFDVILSTVSAPLDFGAYLGLLRTEGTLVNVGAPEEPISLNLFSL
                     ILGNRSIAGSAIGGIAETQEMLDFCAVHGLGAEIEVIEASQVNEAYERVLASDVRYRFV
                     IDTATI"
     aSDomain        complement(1293..2042)
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE135_RS32045_PKS_ER.1"
                     /evalue="8.20E-13"
                     /label="IE135_RS32045_PKS_ER.1"
                     /locus_tag="IE135_RS32045"
                     /protein_end="269"
                     /protein_start="19"
                     /score="40.1"
                     /tool="antismash"
                     /translation="TIERRPVGEFDILIDIKFAGICHSDIHQARDGWGQGIFPMVPGHE
                     IAGIVAETGPGVTRFKVGDRVGVGCMVDSCGQCDACLMGREQHCAEGNTQTYNALDRGG
                     EPTYGGYSTHLVVTEKFAVTIPEGIALDEAAPLLCAGITTYSPLKRWGAGPGKKVAVVG
                     LGGLGHMAVKIAHALGAEVTVLSQSLRKKDDGLKLGADHYYATSDPKTFEELAGTFDVI
                     LSTVSAPLDFGAYLGLLRTEGTLVNVGA"
     gene            2262..3191
                     /locus_tag="IE135_RS32050"
                     /old_locus_tag="GCM10010253_64900"
     CDS             2262..3191
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459219.1"
                     /locus_tag="IE135_RS32050"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_64900"
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_199889758.1"
                     /transl_table=11
                     /translation="MDDQPEAAAPEPLDRRAELSEFLRTRRAKLQPQDVGLPDFGRHRR
                     VPGLRREELAQLAGVSVAYYTRLEQGNGRNVSAEVLDAIARALRLTDAEHAHLNHLARP
                     ARHKKKRRPAKTQRVRTGLLYLLDSMEGIPAYVAGARSDILAWNPMAAAVFGDWAALPA
                     AERNWARLVFLSPAYRDLFLNWDSKASDMVSYLRLYAGCHPDDPQLSALVGELSLKSEE
                     FRRLWATHNVKEKGHGVKLIRHPLVGDLTLSYETLNLPDDEDQQLVTYHAEPGSESAQA
                     LHLLASWGADARRADVISGAGRSADDER"
     gene            complement(3231..3932)
                     /locus_tag="IE135_RS32055"
                     /old_locus_tag="GCM10010253_64910"
     CDS             complement(3231..3932)
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006344625.1"
                     /locus_tag="IE135_RS32055"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_64910"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_199889759.1"
                     /transl_table=11
                     /translation="MTKQERATRTRQALIRSAAVVFEQHGYAQARLVLISSGAGVSTGA
                     LHFHFENKAAVAEAVVAEASQGLRDMSAAIRRRTDTALQALVDTSHALMDRLRGDPVSR
                     AGFRLSCDGERAAAPDLRMEWHHRVRELLDEAAAAGTLAHGIAREEAAAATVAVTAGFE
                     VLGRHDAEWLSPYRLTGFWQLLLPRLATAATLPLLDPAGTGAAPSTRAHALAAFAVKEA
                     DGKAALTAETS"
     gene            complement(4138..4986)
                     /locus_tag="IE135_RS32060"
                     /old_locus_tag="GCM10010253_64920"
     CDS             complement(4138..4986)
                     /codon_start=1
                     /gene_functions="other (smcogs) SMCOG1199: NmrA family
                     protein"
                     /gene_kind="other"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060637.1"
                     /locus_tag="IE135_RS32060"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_64920"
                     /product="NAD(P)H-binding protein"
                     /protein_id="WP_069735027.1"
                     /transl_table=11
                     /translation="MILVTGATGAVGREVAALLAAAGPVRILARSPERVTVRGDGVEVV
                     EGAYGDRPALDRALRGVNSLFLVTNSPTEPDDERVAGAASAAGVRHLVKLSMMAVEEPG
                     ADDFITRRQRENEEAIRESGVAWTFVRPRTFMSNTLSWAPGIRSAGVVRALYGDAPVAC
                     VDPRDVAAVAVAVLTGTGHESRAYAVSGPEAITAREQTAQLSRVLGRPLRFEELGLDAA
                     RAALLAKYPRPVADAFLESAERQRAGAKAAVVPTVQELTGHRARSYRTWSADHADAFAA
                     G"
     gene            complement(5001..5888)
                     /locus_tag="IE135_RS32065"
                     /old_locus_tag="GCM10010253_64930"
     CDS             complement(5001..5888)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060639.1"
                     /locus_tag="IE135_RS32065"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_64930"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_308432670.1"
                     /sec_met_domain="AfsA (E-value: 8.3e-28, bitscore: 88.2,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MVHPVGIDMVHRTRPEDAFPRNWVRLAHDRFSVAAILPHDHPFFA
                     PVGDDRHDPLLVAEAMRQAAMLAFHAGYGVPLGYHFLMADLDYVCHQDHLGVGGTPTEI
                     DVEVFCSDLKWRSGLPVQGRVGWAVHRGGELAATGMGATRFTSPKVYRRMRGDAPAEGI
                     SIPEAAPVAAARAGRSRTEDVVLSDTGREGVWELRVDTRHPTLFQRPNDHVPGMLLLEA
                     ARQAACLAAGPGGIVPAEASTRFHRYAEFGSPCWISAAVLPEEADDTVTVQVTGHQDDE
                     PVFSTLLSGPRADG"
     gene            6106..6744
                     /locus_tag="IE135_RS32070"
                     /old_locus_tag="GCM10010253_64940"
     CDS             6106..6744
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060641.1"
                     /locus_tag="IE135_RS32070"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_64940"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_069735029.1"
                     /transl_table=11
                     /translation="MAKQDRAIRTRQAILMAAARAFEKYGYQAATITEILKSADVTKGA
                     LYFHFPSKEDLALGVLDAQEPPQTVPEQPLKLQELIDLGMLFSHRLQTSLLARAGVRLS
                     MDQQAQGLDRSGPFLRWQEATHHLLAQAKRNGELLPHVNPTETADLYVAAFTGTQAVSQ
                     TLTDYQDLQRRHITLQQHILPSIAAPSILTALDLTPARVERLSLLASEG"
     gene            complement(6783..7748)
                     /locus_tag="IE135_RS32075"
                     /old_locus_tag="GCM10010253_64950"
     CDS             complement(6783..7748)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1287: AraC family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320079.1"
                     /locus_tag="IE135_RS32075"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_64950"
                     /product="GlxA family transcriptional regulator"
                     /protein_id="WP_199889761.1"
                     /transl_table=11
                     /translation="MGNAHRVAVLALDGVYPFELGIPNRVLGAADGRYSVVTCTVDGRP
                     VRTNADFSITVEHGPEVLATADTLVVPPIDTSRLTREAPADAVAALAAVRPGTRIVSIC
                     TGAFLLASAGLLDGRRATTHWAIVELFRTWYPQVRLDPGVLFVDDGDVLTSAGAASGVD
                     VCLHLVRADHGSEVANRVARLCVVPPWRDGGQAQYIEQPVPAPTANDTAATREWALRNL
                     QEPLSLASLAEHARMSRRTFVRRFNEEVGTSPGRWIVQQRVDRARHLLESTDLAVDEIA
                     GRVGFGTGASLRQHLHAAVGVTPLAYRRTFRGAVLAAGQQ"
     gene            7818..8768
                     /locus_tag="IE135_RS32080"
                     /old_locus_tag="GCM10010253_64960"
     CDS             7818..8768
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_ER (26-313). E-value: 3.8e-52.
                     Score: 169.3. Matches aSDomain:
                     nrpspksdomains_IE135_RS32080_PKS_ER.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ADH_zinc_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1028: crotonyl-CoA reductase / alcohol dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060646.1"
                     /locus_tag="IE135_RS32080"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_64960"
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="WP_199889762.1"
                     /sec_met_domain="ADH_N (E-value: 3.3e-10, bitscore: 31.7,
                     seeds: 66, tool: rule-based-clusters)"
                     /sec_met_domain="ADH_zinc_N (E-value: 1.7e-18, bitscore:
                     58.7, seeds: 86, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTKNESMRAISQSVYGGPEVLEEVRLPRPVAGPSQIVVAVRAAGV
                     NPTDWKHRGQTMFLDRLPLVLGWDVSGVVESVGFGVTLFKPGDEVFGMLPYPYGVGSHA
                     EFTTGPARAFAHKPEGVDHVQAGALPLAALTARQALVDTADVRPGQRVLIHAAAGGVGH
                     LAVQIAKSLGAYVIGTASAAKHDFVRGLGADEVVDYRTVDFGDAVRDVDMVLDPLAGET
                     RARSLDVLRPGGTLVSLLPGGDPAEAAKAAAREIRVENLLVEADHAGMTAIADLVRAGE
                     LRAHVEAAFPLAEAAKAHALGETGRTTGKLVLVVS"
     aSDomain        7896..8756
                     /ASF="ER configuration inconclusive"
                     /aSDomain="PKS_ER"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE135_RS32080_PKS_ER.1"
                     /evalue="3.80E-52"
                     /label="IE135_RS32080_PKS_ER.1"
                     /locus_tag="IE135_RS32080"
                     /protein_end="313"
                     /protein_start="26"
                     /score="169.3"
                     /tool="antismash"
                     /translation="PRPVAGPSQIVVAVRAAGVNPTDWKHRGQTMFLDRLPLVLGWDVS
                     GVVESVGFGVTLFKPGDEVFGMLPYPYGVGSHAEFTTGPARAFAHKPEGVDHVQAGALP
                     LAALTARQALVDTADVRPGQRVLIHAAAGGVGHLAVQIAKSLGAYVIGTASAAKHDFVR
                     GLGADEVVDYRTVDFGDAVRDVDMVLDPLAGETRARSLDVLRPGGTLVSLLPGGDPAEA
                     AKAAAREIRVENLLVEADHAGMTAIADLVRAGELRAHVEAAFPLAEAAKAHALGETGRT
                     TGKLVL"
     CDS_motif       8262..8324
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS32080_0001"
                     /evalue="1.90E-08"
                     /label="PKSI-ER_m5"
                     /locus_tag="IE135_RS32080"
                     /protein_end="169"
                     /protein_start="148"
                     /score="26.6"
                     /tool="antismash"
                     /translation="GQRVLIHAAAGGVGHLAVQIA"
     gene            complement(8819..9430)
                     /locus_tag="IE135_RS32085"
                     /old_locus_tag="GCM10010253_64970"
     CDS             complement(8819..9430)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1116:
                     Homoserine/threonine efflux protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459226.1"
                     /locus_tag="IE135_RS32085"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_64970"
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="WP_069735031.1"
                     /transl_table=11
                     /translation="MTNALTTAVTGFGTGLSLIVAIGAQNALVLRQGVRRDGVLAVVGI
                     CALSDALLITLGVAGVGAVVVAWPSALTVVGLLGGLFLIGYGLLAARRVLRPSALHAAD
                     APAASRRGVVLTCLAMTWLNPHVYLDTVFLLGSVAADQGPLRWTFGLGAILASLCWFVT
                     LGFGARLLSGVLARPSAWRVLDGLVAVMMIGLGTKLALGA"
     misc_feature    complement(9481..9496)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 16.36"
                     /tool="antismash"
     misc_feature    9484..9497
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.53"
                     /tool="antismash"
     gene            9498..10439
                     /locus_tag="IE135_RS32090"
                     /old_locus_tag="GCM10010253_64980"
     CDS             9498..10439
                     /GO_component="GO:0005829 - cytosol [Evidence IEA]"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1031: LysR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010060648.1"
                     /locus_tag="IE135_RS32090"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_64980"
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="WP_199889763.1"
                     /transl_table=11
                     /translation="MMSELPLDQVRTLLAVVDEGTFDAAANVLRLTPSAVSQRVKALEQ
                     RTGRVLLMRTKPVRPTESGEVVVRFARQLARLERDARTALGMSGPGEPTSLSIAVNSDS
                     LATWFLPALRRVPQELDVAYELRREDQDHTAALLREGLVMAAVTSSPEAVTGCSVRSLG
                     RMRYLPVASPGFADRWLERRAGAALREVIGDAPVISFDRRDDLQDAFVRRLGPGLRASA
                     RRHLVPTSEGFVSAVAAGMGWGMVPDVHARPLLRDGRLVLLAPDHTVDVPLHWQQWRLD
                     SPALATVAEAVAAEAAETLAAGRPIAPRPVRG"
     misc_feature    10820..10834
                     /note="TFBS match to VqsM, Regulator of quorum-sensing
                     signalling systems, confidence: medium, score: 17.92"
                     /tool="antismash"
ORIGIN
        1 tcaccacggc cgtcaccggg gtgagcaccg tggtcaacgg ggtggtcacg gcggttctgg
       61 cgctcttcct gatgttcttc ttcctaaagg acggcccgcg cttcctcccg tggctcaccc
      121 gtcagcttcc cggcaggctc gccaccgaca tccccgaggt ggccgcccgc agttgggcga
      181 ccctgggcgc gttcgtccgg tcccaggcgt tcgtcggtct gctggacgcg gtcttcatcg
      241 gcctcggtct gtggatcctc ggggtgccgt tggtgctgcc gctggcggtg ctgacattcg
      301 tctgcgcgtt cgtgccgatc gtgggggcgc tgttcgccgg gttcgtggcg gtggtcatcg
      361 ccctggtctc caacggcctg atggacgccc tgctcgtgct ggcgatcatc gtcgtggtgc
      421 agcagctgga gggcaatgtg ttccagccca tgatccagag ccgagggctc ggcctgcacg
      481 cggcggtcgt gctcctcgcg gtgacgctgg gcggcagcct ggccggggtg gtggggagcc
      541 tgctcgccgt gccggtggcg gcggtgctcg cggtggtctg gaactacctc cgcgaacagc
      601 tcgtggagga cccgcgggag ccggagccgg ggccgggatc ggaggcgggg ccggggccgg
      661 ggccggggcc cgaggatctg ccggaggggt cgcccgcgcc gtcgtaggtg tgctgttcgg
      721 acaggctggt gacgcggccg gagggtgact ggcccccggc ccggtgtggg gtcggtggca
      781 gtcgtaccgg tccggcccgt cgggaaacgc tgcctgccgt tcgttgtccg gggtgcgggg
      841 ccgttggtag gccccttccg cggcgagggt gcggtggaag cgttcggcct tgccgctggt
      901 ccggggccgg tagggccgga tcttcttgtg ggcggcgccg gccggggcgg gccgcccgcg
      961 agcgacgagg cagaaagcgt ccaggcgcgt ccggcccgag cacctgacgg gctcgggccg
     1021 gacgctgtcg gcgcagaggc cgccccgggg agcgcgcgtc agatggtcgc ggtgtcgatc
     1081 acgaagcggt agcggacgtc gctcgcgagc acccgctcgt acgcctcgtt gacctggctc
     1141 gcctcgatca cctcgatctc ggcgccgagc ccgtgcacgg cgcagaagtc cagcatctcc
     1201 tgggtctccg cgataccgcc gatcgccgaa cccgcgatcg accggttgcc caggatcagg
     1261 gagaagaggt tcagcgagat cggctcctcg ggggcgccca cgttgacgag ggtgccctcg
     1321 gtgcgcagga gaccgaggta ggcaccgaag tccagcgggg ccgagaccgt ggagaggatg
     1381 acgtcgaagg ttccggccag ttcctcgaag gtcttggggt cgctcgtcgc gtagtagtgg
     1441 tcggcgccca gcttgagccc gtcgtccttc ttccgcaggg actgggagag gaccgtgacc
     1501 tcggcgccga gcgcgtgcgc gatcttgaca gccatgtggc cgagtccgcc gaggccgacg
     1561 acggcgacct tcttgcccgg gcccgcgccc cagcgcttga gcggggagta ggtggtgatg
     1621 cccgcgcaca ggagcggcgc ggcctcgtcg agggcgatgc cctcggggat ggtgacggcg
     1681 aacttctcgg tcacgacgag gtgcgtcgag tagccgccgt aggtgggctc gccgccgcgg
     1741 tcgagagcgt tgtacgtctg ggtgttgccc tcggcgcagt gctgctcgcg gcccatcagg
     1801 caggcgtcgc actgtccgca ggagtcgacc atgcagccga cgcccacccg gtcgccgacc
     1861 ttgaaccggg tgacgccggg gccggtctcg gcgacgatcc cggcgatctc gtggccgggc
     1921 accatcggga agatgccctg gccccagccg tcgcgggcct ggtggatgtc ggagtggcag
     1981 ataccggcga acttgatgtc gatcaggatg tcgaactcgc ccaccggacg acgctcgatc
     2041 gtggtccgct ccagcggggc cttggcacgg ggagcggcgt aggcggcaac ggtggtcatg
     2101 ccggaggttc tcctctgggt gatgcggtag gaacgtgtcc cagcctctct ccgtgtccgg
     2161 cgtttaccca ggccaccgct ctgcctacga ccagtggtcc tactactgac ggggacaggc
     2221 tcggccgcat acgaccacgc ggcacaggaa taatggaacc catggacgat cagccggagg
     2281 ccgccgcacc cgagccgctg gaccggcgtg cggagctcag cgagttcctg cgcacccgga
     2341 gggccaagct ccagccgcag gacgtgggac tgcccgattt cggccggcac cgccgggtgc
     2401 cggggctgcg ccgggaggag ctggcgcagc tggccggggt gtcggtggcg tactacacgc
     2461 gtctggagca gggcaatggg cgcaatgtgt cggcggaggt gctggacgcg atcgcgcgtg
     2521 cgctgcggct cacggacgcc gagcacgcgc atctgaacca tctggcccgg ccggcccggc
     2581 acaagaagaa gcgccgcccg gcgaagacgc agcgcgtacg gaccgggctg ctgtatctcc
     2641 tcgacagcat ggagggcatc cccgcgtacg tggccggggc gcggtccgac atcctcgcct
     2701 ggaatccgat ggctgcggcg gtgttcggcg actgggccgc gctgccggcg gccgagcgga
     2761 actgggcccg tctggtgttc ctctcccccg cctaccggga cctgttcctg aactgggact
     2821 ccaaggcctc ggacatggtc agctatctcc ggctgtacgc ggggtgccac cccgacgacc
     2881 cgcagttgtc ggcgctggtg ggcgaactct cactgaagag cgaggagttc cggcggttgt
     2941 gggcgacgca caacgtcaag gagaagggcc acggcgtcaa gctgatccgt catccgctcg
     3001 tcggcgacct gaccctgtcg tacgaaacgc tgaatctgcc ggacgacgag gaccagcagc
     3061 tggtgaccta tcacgcggag ccggggtcgg agtcggcgca ggccctgcac ctgctggcga
     3121 gctggggagc cgacgcgcgc cgggctgatg tgatcagcgg cgcgggccgg agtgcggacg
     3181 acgagcgatg agggccgacc tcgttccacg atcggtcgat tgacgggcag tcagcttgtc
     3241 tccgccgtca gcgccgcctt cccgtccgcc tctttgacgg cgaacgccgc gagggcgtga
     3301 gcccgggtcg acggagccgc acccgtcccg gccgggtcca ggagcggcag ggtcgccgcg
     3361 gtggcgagcc ggggcagcag caactgccag aagccggtga gccggtacgg ggacagccac
     3421 tcggcgtcgt ggcggccgag cacctcgaat ccggccgtca cggcgacggt cgcggctgcc
     3481 gcttcctcgc gggcgatgcc gtgggcgagc gttccggcgg cggcggcctc gtccagcagt
     3541 tcccgcaccc ggtggtgcca ctccatccgc aggtccgggg cggcggcccg ctcgccgtcg
     3601 cagctcagcc ggaacccggc gcggctcacc gggtccccgc gcagccggtc catgagggcg
     3661 tgcgaggtgt cgacgagtgc ttgcagggcg gtgtccgtcc tgcgccggat cgcggcggac
     3721 atgtcgcgca gcccttggga cgcctccgcc accacggctt ccgcgaccgc ggccttgttc
     3781 tcgaagtgga agtgcagcgc ccccgtgctg acgcccgcgc cggagctgat cagcaccagc
     3841 ctggcctggg cgtaaccgtg ctgttcgaag acgacggcgg cggagcggat gagcgcctgt
     3901 cgggtccggg tggcgcgctc ctgtttggtc atggagttat caaaccaact taccggtttc
     3961 atttccaggg aacggaagat tacgccagag gcccttgagc ccgctttccg gccgtgttcg
     4021 ggcacgcgag ccaccggaaa cggaccggga aagaagaagc gcacggggtc ggcgcattcc
     4081 gcagcccgtt gggcaggcgg gcagtcggcg tgctttcgcc catgcggggt gccgccccta
     4141 tccggcggcg aacgcgtccg cgtggtcggc cgaccaggtg cggtacgacc tggcccggtg
     4201 tcccgtcagc tcctggaccg tggggaccac ggccgccttc gcgcccgccc gctgtcgctc
     4261 ggcgctctcc aggaaggcat ccgcgacggg ccgcgggtat ttcgccagca gggccgcgcg
     4321 tgcggcgtcc aggcccagtt cctcgaagcg cagcggacgc cccaacaccc gggagagctg
     4381 cgccgtctgt tcccgcgcgg tgatcgcctc cgggcccgag accgcgtacg ccctgctctc
     4441 gtggcccgtg ccggtgagca ccgcgacggc caccgcggcg acgtcccggg ggtcgacgca
     4501 cgcgacgggg gcgtcgccgt acagagcgcg taccactccc gccgagcgga tgccgggcgc
     4561 ccaggacagc gtgttggaca tgaacgtcct ggggcggacg aacgtccagg cgaccccgga
     4621 ctccctgatc gcctcctcgt tctcccgctg acgtcgggtg atgaagtcgt cggcccccgg
     4681 ttcctccacc gccatcatgg agagcttgac caggtggcgg acgcccgccg cggaggccgc
     4741 cccggcgacc cgctcgtcgt cgggctccgt ggggctgttc gtcaccagga acagcgagtt
     4801 gacgccccgc agggcccggt cgagggcggg gcggtccccg tacgccccct cgacgacctc
     4861 gaccccgtcc ccgcgtaccg tcacacgctc agggctgcgg gcgagaatcc gtacgggacc
     4921 ggcggccgcc agcagtgcgg cgacctcgcg gcccaccgct ccggtcgcac ccgtcacgag
     4981 aatcactggg aagagacctc tcagccgtcg gcccgggggc cggacaggag tgtggagaag
     5041 accggttcat cgtcctgatg acctgtcacc tggaccgtca cggtgtcgtc ggcctcttcc
     5101 ggcaggaccg ccgcgctgat ccagcacggg ctgccgaact cggcgtaccg gtggaaccgg
     5161 gtgctggcct ccgccgggac gatgccgccg ggacctgccg cgaggcaggc cgcctgacgg
     5221 gccgcctcca gcagcagcat gcccgggaca tggtcgttgg ggcgctggaa cagcgtcggg
     5281 tgccgggtgt ccacgcgtag ctcccagacg ccttcgcggc cggtgtccga gagcaccacg
     5341 tcctcggtcc tgctccggcc cgcacgggcg gcggcgaccg gcgccgcctc gggtatcgat
     5401 atcccctcgg cgggggcgtc gcctcgcatc cgccggtaca ccttggggct ggtgaaacgg
     5461 gtcgcgccca tccctgtggc cgccagttcc ccgccccggt gcaccgccca gcccacgcgc
     5521 ccctgcacgg gcagtccgct ccgccacttc aggtcggagc agaacacctc cacgtctatc
     5581 tcggtggggg ttccgccgac gcccaagtgg tcctggtggc acacgtagtc caggtccgcc
     5641 atcaggaagt ggtagccgag cggaacgccg tacccggcgt ggaaggcgag catggccgcc
     5701 tgccgcatcg cttcggccac cagcagcgga tcgtgccggt cgtcgccgac cggggcgaaa
     5761 aacgggtgat cgtgcggcag gatcgccgcc accgagaacc gatcgtgcgc cagacggacc
     5821 cagttgcggg gaaacgcgtc ctccggcctg gtccggtgca ccatgtcgat ccccacggga
     5881 tgcaccatgt cggcctccga atccattgcc ggcaccgagt cgatcagggc tgctgcttcg
     5941 ggcataactc cccccagggg tcatgtgatg gcgagccgat tctgcgatgc acgtgcgctg
     6001 ataagataca gactcagcgg ttttttttct atccccggca gggtcgcccc tccccacacc
     6061 gtcccggccg tttcccgggc catccggccc agcaaaggag acaggatggc caagcaggac
     6121 cgggcgatcc gcacccgcca ggcaatcctg atggcagcgg cgcgggcttt cgagaaatac
     6181 ggctatcaag ccgcgacgat caccgaaata ctcaaaagcg ccgacgtgac gaaaggcgct
     6241 ctctactttc attttccgtc caaagaagat ctcgcgctcg gtgttctcga tgcccaggag
     6301 ccgccgcaga ccgttccgga gcagcccctc aaactgcagg aactcatcga cctggggatg
     6361 ctgttcagcc accgcctgca gaccagtctc ctcgcccggg cgggcgtgcg cctctccatg
     6421 gaccagcagg cacagggcct cgaccgcagc ggccctttcc tccgctggca ggaagccacc
     6481 catcacctcc tcgcgcaggc caaacgcaac ggtgagctgc tcccccacgt caaccccacc
     6541 gagacggccg acctgtacgt ggccgccttc acgggcaccc aggccgtatc ccagactctc
     6601 accgactacc aggacctgca gcgccgccac atcacgctcc agcagcacat acttcccagc
     6661 atcgccgcac cctcgatcct caccgccctc gatctgacgc ccgcgcgcgt cgaacgcctc
     6721 agcctcctgg cctccgaggg ctgaaccggg ggccgggcct gtgccggccc ccgcaccccc
     6781 gtctactgct gtccggccgc gaggaccgcg ccacgaaacg tacgccgata ggccagtggg
     6841 gtgacgccga cggcggcatg cagatgctgg cgcagcgacg cgccggtgcc gaacccgacc
     6901 cggcccgcga tctcgtccac cgccaggtcc gtggactcca ggagatgccg ggcccggtcg
     6961 acccgctgct ggacgatcca ccggcccggg ctcgtgccga cctcctcgtt gaaacgtcgg
     7021 acgaaggtgc gccggctcat ccgggcgtgc tcggcgaggc tcgccaggct cagcggctcc
     7081 tggaggttcc gcagcgccca ttcgcgggtc gcggcggtgt cgttcgccgt cggagcgggc
     7141 accggctgct cgatgtactg ggcctgtccc ccgtcgcgcc agggcggtac gacgcagagc
     7201 cgggcgaccc ggttggcgac ctcgctgccg tggtccgcgc ggacgaggtg caggcagacg
     7261 tcgacgccgg aggccgcgcc cgccgaggtc aggacgtccc cgtcgtcgac gaagaggacg
     7321 cccgggtcga gccgcacctg ggggtaccag gtgcggaaga gctccacgat cgcccagtgg
     7381 gtggtggccc gccggccgtc cagcagtccg gccgaggcga ggaggaaggc gccggtgcag
     7441 atggacacga tgcgggtgcc cgggcgtacg gccgccagcg ccgcgacggc gtccgcgggc
     7501 gcctcacggg tgaggcgaga ggtgtcgatg ggcggaacga cgagggtgtc cgccgtcgcc
     7561 agcacctcgg ggccgtgttc gacggtgatc gagaagtcgg cgttggtgcg cacgggccgg
     7621 ccgtccaccg tgcaggtcac gacggagtag cggccgtcgg ccgcgccgag gacccggttc
     7681 gggatgccga gctcgaacgg atacaccccg tcgagggcga gtacggcgac gcggtgcgcg
     7741 tttcccatgg cacgatcctg tcgaaagatg gcaatcatgc catggtacgc cgggcggaca
     7801 ggcgtgaggc tggacgtatg acgaagaacg agagcatgcg cgccatcagc cagagcgtct
     7861 acggaggtcc cgaggtactg gaggaggtcc ggcttccccg gccggtcgcg ggaccgagcc
     7921 agatcgtggt cgccgtccgg gcggccggag tgaaccccac ggactggaag caccggggcc
     7981 agacgatgtt cctggaccgg ctgccgctcg tcctgggctg ggacgtgtcc ggggtcgtgg
     8041 agtcggtggg cttcggggtg accctcttca agccgggcga cgaggtgttc ggcatgctgc
     8101 cctacccgta cggtgtcggc tcccacgccg agttcacgac cggcccggcc cgcgccttcg
     8161 cccacaagcc cgagggcgtc gaccatgtgc aggcgggcgc cctcccgctc gccgccctca
     8221 ccgcccgcca ggcgctggtc gacacggcgg acgtgcgccc cgggcagcgg gtgctgatcc
     8281 acgccgccgc cggcggggtc ggccatctcg ccgtacagat cgccaagtcg ctgggggcgt
     8341 acgtcatcgg caccgcgagc gccgccaagc acgacttcgt gcgcgggctc ggggcggacg
     8401 aggtcgtcga ctaccgcacc gtggacttcg gggacgcggt gcgcgatgtc gacatggtgc
     8461 tcgacccgct cgcgggcgag acccgggcgc gttcgctcga tgtcctgcgc ccgggaggca
     8521 cgctcgtgtc gctgctgccc ggcggcgatc cggccgaggc ggcgaaggcg gccgcgcggg
     8581 agatccgggt ggagaacctg ctggtcgagg cggaccacgc gggcatgacg gccatcgccg
     8641 acctggtccg ggccggtgag ctgcgcgccc acgtcgaggc ggccttccca ctggccgagg
     8701 cggccaaggc gcacgcgctg ggcgaaacgg gccgcaccac cggcaagctc gtcctcgtgg
     8761 tgtcgtagcc cgcggggcgc ccggcgtctc ctccccgcga ggacgaggcg ccgggcgttc
     8821 aggcgcccag cgccagtttc gtaccgaggc cgatcatcat gacggcgacc aggccgtcca
     8881 gcacccgcca ggcggagggg cgcgcgagga ctccgctcag cagccgggcg ccgaacccga
     8941 gcgtcacgaa ccagcacagg ctggcgagga tcgcgccgag gccgaaggtc cagcgcaggg
     9001 gcccctggtc ggcggcgacg gacccgagca ggaacacggt gtcgaggtac acgtgcgggt
     9061 tgagccacgt catcgccagg caggtcagca cgacaccccg acgcgacgcg gccggggcgt
     9121 ccgccgcgtg cagggcggac gggcgcagca cgcggcgggc ggcgagcagc ccgtagccga
     9181 tcaggaacag gcctccgagc agaccgacga cggtcagcgc cgagggccag gcgacgacga
     9241 ccgcgccgac gccggcgacc cccagcgtga tgagcaacgc gtcggacagg gcgcagatgc
     9301 cgaccaccgc gagcacgccg tcccggcgga ccccctggcg cagtaccagg gcgttctggg
     9361 cgccgatggc gacgatgagg gagaggccgg taccgaaacc ggtgactgcg gtggtcaggg
     9421 cgttcgtcat acggccgacg ctacggagcg acgcaacccg ctgtccaact aaagattctt
     9481 acgtatcatt agcattcatg atgtccgagc ttcccctcga tcaggtgcgc accctgttgg
     9541 ccgtggtcga cgaaggcacg ttcgacgcgg cggcgaacgt actgcggctg acaccgtcgg
     9601 cggtcagcca gcgggtgaag gcactggagc aacgcacggg ccgggtgctg ttgatgcgta
     9661 cgaagccggt gcggcccacc gagtccggtg aggtggtggt gcgcttcgcc cgccagctgg
     9721 cccggctgga gcgcgacgcg cggacggcac tcggcatgtc cgggcccggc gaaccgacat
     9781 ccctgtcgat cgcggtgaac tccgattccc tggcgacctg gttcctgccc gccctgcggc
     9841 gcgtaccgca ggaactggac gtcgcctacg aactgcgcag ggaggaccag gaccatacgg
     9901 ccgccctgtt gcgggagggg ctggtgatgg ccgcggtgac ctcgtcgccg gaggcggtga
     9961 cggggtgttc ggtgcgcagt ctcggccgta tgcggtatct gccggtggcc agtcccggat
    10021 tcgccgaccg gtggctggag aggcgggcgg gcgcggcgtt gcgcgaggtg atcggcgacg
    10081 ccccggtcat cagcttcgac cggcgcgacg atctgcagga cgccttcgtg cgccggctcg
    10141 gacccggcct ccgggcgagc gcccggcgtc atctggtgcc cacgtcggag gggttcgtga
    10201 gcgccgtggc ggcgggcatg ggctggggca tggtgcccga cgtccatgcg aggccgttgc
    10261 tgcgcgacgg ccggctggtg ctgctcgccc cggaccacac cgtggacgtc cccctgcact
    10321 ggcagcagtg gaggctggat tcaccggcgc tggcgaccgt ggccgaggcg gtggcggcgg
    10381 aggccgccga gacgctggcg gccggacggc cgattgcccc tcgacccgtt cggggttagg
    10441 ttcacctaac tcaacaggga ggtgcgaaac atgaccgacg gtcaggaccg ggagccccgc
    10501 aacagcgagg aaccggcgac ggattgcagg cacgcggagc ggtggcctgg ttcgtgctcg
    10561 gcctgatgac gacccaggcg ctgggcgcgg cggtgcggtt cgacctcttc gaccgatcgg
    10621 gccgggctcc ctgagcgccg acgcgctcgc cgaatccctc ggcacccacc cacaggccac
    10681 gctgcgcctg ctgcgcgccc tggccggact ccacctgctg tccgagacgg aaccgggcgt
    10741 tttccatgct gcggggctgg aacctgctcg acgagagcgt acggacgggc cggacgacgt
    10801 tcgacatggt gttcgggacc gacttcttcg gccatctgaa ggagcacccc gagctgtccg
    10861 ccgcgttcaa tgaggcgatg acccaggg
//
