LOCUS       NZ_BMSZ01000021        10399 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces badius strain JCM 4350 sequence21, whole genome shotgun
            sequence.
ACCESSION   NZ_BMSZ01000021
VERSION     NZ_BMSZ01000021.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces badius
  ORGANISM  Streptomyces badius
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 116069)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 154X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649415.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:57:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,894
            CDSs (total)                      :: 6,825
            Genes (coding)                    :: 6,582
            CDSs (with protein)               :: 6,582
            Genes (RNA)                       :: 69
            rRNAs                             :: 2 (23S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 70 of 244
            Pseudo Genes (incomplete)         :: 206 of 244
            Pseudo Genes (internal stop)      :: 7 of 244
            Pseudo Genes (multiple problems)  :: 38 of 244
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:11:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 37135
            Orig. end                         :: 47534
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSZ01000021.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10399
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10399
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10399
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5399](-)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..5399)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     gene            81..1139
                     /locus_tag="IE135_RS29740"
                     /old_locus_tag="GCM10010253_60150"
     CDS             81..1139
                     /NRPS_PKS="Domain: Aminotran_5 (19-347). E-value: 1.5e-23.
                     Score: 75.2. Matches aSDomain:
                     nrpspksdomains_IE135_RS29740_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070339.1"
                     /locus_tag="IE135_RS29740"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_60150"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_069739477.1"
                     /sec_met_domain="Aminotran_5 (E-value: 1.4e-18, bitscore:
                     59.9, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTILAPIQAAAVEFAPEVTYLNTSSWGLLPRRTIAAVKALADENA
                     AGRRVGAGSFEAVEAARAGFARLVGVHPDRVATGSSVTVHVGLIAASLPPGAEVLCPEG
                     EFSSVVSPFALRGDLRMRYAPLADLAGAVRASTALVAFSAVQSADGRVADLAAVRAAAS
                     AHGARTLLDATQAAGWFPVDAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAQDTLPALFA
                     GWVSAGAPWTNNYGPLERLAPTARGFDEPPAFLSYHGAEHSLALLAEVGAEALYAHATG
                     LAARLRTGLAALGHKAVPGESAIVAVPGLDGRQPELVEAGIAVSAPAGNLRISCHLHNT
                     EADVDRLLDFLA"
     aSDomain        138..1121
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE135_RS29740_Aminotran_5.1"
                     /evalue="1.50E-23"
                     /label="IE135_RS29740_Aminotran_5.1"
                     /locus_tag="IE135_RS29740"
                     /protein_end="347"
                     /protein_start="19"
                     /score="75.2"
                     /tool="antismash"
                     /translation="YLNTSSWGLLPRRTIAAVKALADENAAGRRVGAGSFEAVEAARAG
                     FARLVGVHPDRVATGSSVTVHVGLIAASLPPGAEVLCPEGEFSSVVSPFALRGDLRMRY
                     APLADLAGAVRASTALVAFSAVQSADGRVADLAAVRAAASAHGARTLLDATQAAGWFPV
                     DAGAYDYTVTGGFKFLLCPRGTSFLTVTEEAQDTLPALFAGWVSAGAPWTNNYGPLERL
                     APTARGFDEPPAFLSYHGAEHSLALLAEVGAEALYAHATGLAARLRTGLAALGHKAVPG
                     ESAIVAVPGLDGRQPELVEAGIAVSAPAGNLRISCHLHNTEADVDRL"
     gene            complement(1176..1697)
                     /locus_tag="IE135_RS29745"
                     /old_locus_tag="GCM10010253_60160"
     CDS             complement(1176..1697)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1284:
                     transcriptional regulator, MarR family"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123628.1"
                     /locus_tag="IE135_RS29745"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_60160"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_069739476.1"
                     /transl_table=11
                     /translation="MTTGRATPAEDACTNAPPLAALGGPVSHAVSRVARLHRIAAGKAL
                     RGVDLYPGQELLMMHLWDRGAVRQTELIRALELDPSTVTKMLQRLEQTGHVRRRPDPAD
                     RRAVLVEATDESCALHTAVEAAWGSLEEHTLAGLDPAERTELVRLLAKVEENLCRETED
                     CPDRRGAASA"
     gene            1874..2944
                     /locus_tag="IE135_RS29750"
                     /old_locus_tag="GCM10010253_60170"
     CDS             1874..2944
                     /GO_function="GO:0010181 - FMN binding [Evidence IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1217: NADH:flavin oxidoreductase/NADH oxidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003969934.1"
                     /locus_tag="IE135_RS29750"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_60170"
                     /product="alkene reductase"
                     /protein_id="WP_199889557.1"
                     /transl_table=11
                     /translation="MTTVFDPIDLSGTPLANRIALAPMTRSRAGEGGTATELTATYYAQ
                     RASAGLIITEGIQPSVVGQGYPSTPGLHSAEQVASWRAVTDAVHAKGGRIFAQIMHAGR
                     IGHPVLLPDGLVPVSASPVKAEGQVYTHVGPKDFVEPHELTDAEVRATVADFVTAARNA
                     VAAGFDGVELHGANGYLIHQFLAPNTNRRTDAWGGSDEARTRFAVEVVEAVAAEIGPER
                     TGLRISPGNPYNDIDEPAPEATYTALVRAIEPLGLAYLHILEATPIRELTLALRKAFSG
                     TVVINVHSDGPAGPGDHTVIDDGIADLISYGALFLANPDLPARLRAGGPFNTPDPSTFF
                     GGDAKGYTDYPALDAV"
     misc_feature    1875..1889
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 17.18"
                     /tool="antismash"
     gene            complement(2920..3954)
                     /locus_tag="IE135_RS29755"
                     /old_locus_tag="GCM10010253_60180"
     CDS             complement(2920..3954)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010070337.1"
                     /locus_tag="IE135_RS29755"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_60180"
                     /product="VOC family protein"
                     /protein_id="WP_199889558.1"
                     /transl_table=11
                     /translation="MTSHVRHITIDCADAYALAAFWAQVLGAPLSDEDSPGDPEALVEA
                     PGAAILFVQVDEPKQTKNRVHLDIQPDDRSRDEEVERLLALGATLVADHRKPNGRGWAT
                     LADPEGNEFCVECGADERAALTGTRLPVTADDVTTAVRLAVDVLAKAPADRWEARAGSL
                     EWTCWETAEHLGDDLVSYAVRLGPRTPPMEQNLPFRWTSERPGGHDGLLFVDRSAGPAG
                     LLATVEARGALLASVVRTTPPEVRAYHVYSVSDPEGFAAMGVVETLVHAHDIAEGLGLT
                     WEPPAALCDRALARLFPDAPAGGDRWAVLLWATGRAELPGHPRRTAWRWDGRPLEDQTA
                     SSAG"
     gene            complement(4104..4997)
                     /gene="thpD"
                     /locus_tag="IE135_RS29760"
                     /old_locus_tag="GCM10010253_60190"
     CDS             complement(4104..4997)
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156966.1"
                     /locus_tag="IE135_RS29760"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_60190"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_069739473.1"
                     /sec_met_domain="PhyH (E-value: 4.7e-40, bitscore: 131.0,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTTEVRADLYPSRGAAEMTTPRQDPVIWSAPGAPGPIAAKDLQSY
                     EHDGFLSIEQLITPDEVAVYRAELDRLVADPLIRADERSIVEKQSQNVRSVFEVHKISE
                     VFAGLVRDERVVGRARQILGSDVYVHQSRINVKPGFGASGFYWHSDFETWHAEDGLPNM
                     RTVSVSIALTENFDTNGGLMIMPGSHKTFLGCAGETPKDNYKKSLQMQDAGTPSDEALT
                     KMADRHGIKLFTGKAGSATWFDCNAMHGSGDNITPYARSNVFIVFNSVENAAQEPFAAP
                     IRRPEFIGARDFTPVR"
     gene            complement(5001..5399)
                     /locus_tag="IE135_RS29765"
                     /old_locus_tag="GCM10010253_60200"
     CDS             complement(5001..5399)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123624.1"
                     /locus_tag="IE135_RS29765"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_60200"
                     /product="ectoine synthase"
                     /protein_id="WP_006123624.1"
                     /sec_met_domain="ectoine_synt (E-value: 2.2e-55, bitscore:
                     178.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFSDIENTDRHVKAASGTWESKRIVLAKEKVGFSLHETVLY
                     AGTETSMWYANHIEAVLCTEGEAELTNDETGEKHWITPGTMYLLDGHERHTMRPKTDFR
                     CVCVFNPPVTGREDHDENGVYPLLTEEG"
     gene            complement(5489..6751)
                     /gene="ectB"
                     /locus_tag="IE135_RS29770"
                     /old_locus_tag="GCM10010253_60210"
     CDS             complement(5489..6751)
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-363). E-value: 6.6e-72.
                     Score: 234.3. Matches aSDomain:
                     nrpspksdomains_IE135_RS29770_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156968.1"
                     /locus_tag="IE135_RS29770"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_60210"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_069739472.1"
                     /sec_met_domain="Aminotran_3 (E-value: 7e-78, bitscore:
                     254.9, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITPPALSVFETLESEVRSYCRGWPAVFDRAQGARLTDEDGHSY
                     LDFFAGAGSLNYGHNNPVLKRALIDYIERDGITHGLDMATTAKRAFLETFQNAILRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGRESVVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GIPLVHGTPMPFDNYFDGTVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARA
                     EWLRALKELCERQDMLLIVDDIQMGCGRTGGFFSFEEAGIVPDIVTLSKSISGYGLPMA
                     LCLFKPELDIWEPGEHNGTFRGNNPAFVTAAAALQAYWADGQMEKQTLARGEQVEQTLL
                     AICAEEPTAQFRGRGLVWGMEFEDPARASAVCARAFELGLLLETSGPQSEVVKLLPPLT
                     ITPEELDEGLRTLARSVRETA"
     aSDomain        complement(5663..6667)
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE135_RS29770_Aminotran_3.1"
                     /evalue="6.60E-72"
                     /label="IE135_RS29770_Aminotran_3.1"
                     /locus_tag="IE135_RS29770"
                     /protein_end="363"
                     /protein_start="28"
                     /score="234.3"
                     /tool="antismash"
                     /translation="FDRAQGARLTDEDGHSYLDFFAGAGSLNYGHNNPVLKRALIDYIE
                     RDGITHGLDMATTAKRAFLETFQNAILRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     RESVVSFTNAFHGMSLGSLAVTGNAFKRAGAGIPLVHGTPMPFDNYFDGTVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARAEWLRALKELCERQDMLLIVDDIQMGCGR
                     TGGFFSFEEAGIVPDIVTLSKSISGYGLPMALCLFKPELDIWEPGEHNGTFRGNNPAFV
                     TAAAALQAYWADGQMEKQTLARGEQVEQTLLAICAEEPTAQFRGRGLVWGMEFE"
     gene            complement(6933..7463)
                     /gene="ectA"
                     /locus_tag="IE135_RS29775"
                     /old_locus_tag="GCM10010253_60220"
     CDS             complement(6933..7463)
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123622.1"
                     /locus_tag="IE135_RS29775"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_60220"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_079134193.1"
                     /transl_table=11
                     /translation="MTAAPADLARARSEFLSIDAPRVEDGAAIWRIARDSQVLDLNSSY
                     SYLLWCRDFAATSVVARGESGEPIAFVTGYIRPDRPQTLVVWQVAVDAAHRGKGLAATL
                     LDALTARVAADQGLSSVETTITPDNTASDRLFTSYAQRHDVALEQEVLFDVDLFPEGTH
                     LPEVLYRIGPFAT"
     misc_feature    7474..7488
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 15.51"
                     /tool="antismash"
     misc_feature    complement(7488..7500)
                     /note="TFBS match to LuxO, Repressor of luminescence,
                     confidence: weak, score: 17.04"
                     /tool="antismash"
     misc_feature    complement(7517..7532)
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.74"
                     /tool="antismash"
     misc_feature    complement(7619..7634)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(7629..7646)
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 14.75"
                     /tool="antismash"
     misc_feature    7704..7720
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 20.46"
                     /tool="antismash"
     misc_feature    7737..7750
                     /note="TFBS match to MogR, Repressor of flagellar motility
                     genes, confidence: weak, score: 13.49"
                     /tool="antismash"
     gene            complement(7774..8880)
                     /locus_tag="IE135_RS29780"
                     /old_locus_tag="GCM10010253_60230"
     CDS             complement(7774..8880)
                     /NRPS_PKS="Domain: Aminotran_5 (19-284). E-value: 4.5e-27.
                     Score: 86.8. Matches aSDomain:
                     nrpspksdomains_IE135_RS29780_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006123620.1"
                     /locus_tag="IE135_RS29780"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_60230"
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="WP_069743210.1"
                     /sec_met_domain="Aminotran_5 (E-value: 4.6e-22, bitscore:
                     71.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLPPLTAGHFAAIERRVARLLATEQDVVITQGEALLPLE
                     GCIRGGARPGSTALNVVTGPYGQTFGNWLRDCGAQVVDLAVPFHTAVTADQVAEALAGH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVGAHGALFMLDAVASVGAEPLLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSARAWERIAANPAAPRRSYLSLLDWKERWIDGGRTALPHAP
                     AQLEMLGLEACVARIEDEGLETVMARHASAAAATRAGAVALGGGLEPYVHEARDAAPVA
                     TTLRAPAGTDAAALVAAALAADPALPLVAGGGALSKEMIRVNHYGADATRGVVLSSLAA
                     LGAALADGGQQVDIEAARRAVSETWPSR"
     aSDomain        complement(8029..8823)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE135_RS29780_Aminotran_5.1"
                     /evalue="4.50E-27"
                     /label="IE135_RS29780_Aminotran_5.1"
                     /locus_tag="IE135_RS29780"
                     /protein_end="284"
                     /protein_start="19"
                     /score="86.8"
                     /tool="antismash"
                     /translation="ERRVARLLATEQDVVITQGEALLPLEGCIRGGARPGSTALNVVTG
                     PYGQTFGNWLRDCGAQVVDLAVPFHTAVTADQVAEALAGHPEIDFVSLVHAEAATGNTN
                     PVAEIGEVVGAHGALFMLDAVASVGAEPLLPDAWGVDLCVIGAQKAMGGPAGVSAVSVS
                     ARAWERIAANPAAPRRSYLSLLDWKERWIDGGRTALPHAPAQLEMLGLEACVARIEDEG
                     LETVMARHASAAAATRAGAVALGGGLEPYVHEARDAAPVATTL"
     gene            9029..10105
                     /locus_tag="IE135_RS29785"
                     /old_locus_tag="GCM10010253_60240"
     CDS             9029..10105
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Amidohydro_1"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018512211.1"
                     /locus_tag="IE135_RS29785"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_60240"
                     /product="amidohydrolase family protein"
                     /protein_id="WP_199889574.1"
                     /sec_met_domain="Amidohydro_1 (E-value: 3.1e-18, bitscore:
                     59.0, seeds: 57, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLRVKGRVLVGPDEVRDELWAVDGRITFERPPGADDAVTVRGWAL
                     PGLVDAHCHVGLDRHGPVDAATAEKQALTDREAGTLLIRDAGSPSDTRWIDDREDLPKI
                     IRAGRHIARTRRYIRNYAHEIEPGDLVAYVAQEARRGDGWVKLVGDWIDRDAGDLTACW
                     PCGEVEAAIAEAHRLGARVTAHCFAEESLRDLVEAGIDCVEHATGLTEDTIPLFAERGV
                     AIVPTLVNIATFPDLAAGGEAKFPRWSAHMRQLYDRRYDTVRSAYDAGIPVYVGTDAGG
                     SLAHGLVSGEVAELVKAGIPPLEALSATAWGARRWLGRPGLDEGAPADLVVYDADPRAD
                     VRVLAAPRHIVLNGRVIG"
     misc_feature    10243..10258
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 21.67"
                     /tool="antismash"
ORIGIN
        1 gcgatccata agcacttctt tggagttccc cttaactctc tgcaattgac tttgggtcga
       61 gggggctcca gggtggacgc atgacgatcc tcgcgcccat ccaagccgca gccgtcgaat
      121 tcgcgcccga ggtcacctac ctcaacacct ccagctgggg cctgctgccc cgccggacga
      181 tcgccgccgt caaggccctg gccgacgaga acgccgcggg ccgccgggtg ggggccggca
      241 gcttcgaggc cgtggaggcc gcccgggccg gcttcgcgcg gctcgtcggg gtccacccgg
      301 accgggtcgc caccggcagc tccgtcaccg tccacgtcgg cctgatcgcc gcctcgctgc
      361 cgcccggtgc cgaagtcctc tgccccgagg gcgagttctc ctccgtcgtc agcccgttcg
      421 cgctccgcgg cgatctgcgc atgcggtacg cgccgctcgc ggacctcgcc ggggccgtcc
      481 gcgcatcgac cgcgctggtc gccttctccg ccgtgcagtc ggccgacggc cgggtcgccg
      541 acctcgccgc cgtccgcgcg gccgcctcgg cgcacggggc ccgcaccctg ctggacgcca
      601 cccaggcggc gggctggttc ccggtggacg ccggggcgta cgactacacc gtgaccggcg
      661 gcttcaaatt cctgctctgc ccgcgcggta cgtcgttcct gaccgtcacc gaggaggcgc
      721 aggacaccct gccggccctc ttcgcgggct gggtctccgc gggtgccccc tggacgaaca
      781 actacggccc gctggagcgc ctggccccca ccgcccgcgg cttcgacgag ccgcccgcct
      841 tcctctcgta ccacggggcc gagcactccc tcgccctgct cgccgaggtc ggcgccgagg
      901 ccctgtacgc gcacgccacc gggctcgccg cccggctgcg gacggggctg gcggccctcg
      961 ggcacaaggc cgttcccggg gagtcggcga tcgtcgccgt gccggggctc gacgggcgtc
     1021 agcccgagct ggtggaggcc gggatcgccg tctccgcccc ggccgggaac ctgcggatct
     1081 cctgccacct ccacaacacc gaggccgatg tggaccggct gctggacttc ctggcctgag
     1141 ccgtatccgc acgggcgtgg ctcgggccgc acggctcagg cggacgcggc cccgcgccgg
     1201 tcggggcagt cctcggtctc ccggcagagg ttctcctcga ccttcgccag cagccgcacc
     1261 agctccgtgc gctcggccgg gtccaggccc gcgagcgtgt gctcctccag gctgccccag
     1321 gcggcctcca ccgcggtgtg cagcgcgcag ctctcgtccg tcgcctcgac cagcacggcc
     1381 cgccggtcgg ccgggtcggg gcggcggcgc acatgcccgg tctgctccag ccgctggagc
     1441 atcttcgtga ccgtggacgg gtccagctcc agggccctga tcagctccgt ctgccggacc
     1501 gccccccggt cccacaggtg catcatcagc aactcctgcc ccgggtagag gtccaccccc
     1561 ctgagcgcct tgcccgccgc gatccggtgc agccgggcca cccgggagac ggcgtggctg
     1621 acgggcccgc cgagcgccgc caggggcggc gcgttcgtac aggcgtcttc cgcgggggtg
     1681 gcgcggccgg tggtcatggc gggctcctgg ggtcgtcggg gtggagagcg acggtttcgg
     1741 tgatggcctc ggtggcggct tccgatgatg cccgacggtt tcggacaact ttaccttggt
     1801 cggccaagga atgggttaca gttgctcccg gcggcgatgg ttggccgacc acatacttat
     1861 ccgggagccc ctcatgacca ccgttttcga cccgatcgac ctgtccggca ccccgctggc
     1921 caaccgcatc gcgctggccc cgatgacccg cagccgggcc ggtgagggcg gcaccgccac
     1981 cgagctgacc gccacgtact acgcccagcg cgcctcggcg ggactcatca tcaccgaggg
     2041 catccagccg tccgtcgtgg gccagggcta cccctccacg cccggcctgc acagcgccga
     2101 gcaggtcgcc tcctggcgtg cggtcaccga cgcggtgcac gcgaagggcg gcaggatctt
     2161 cgcccagatc atgcacgcgg gccgtatcgg ccacccggtc ctgctgccgg acgggctcgt
     2221 ccccgtcagc gcctccccgg tcaaggccga ggggcaggtc tacacccacg tcggaccgaa
     2281 ggacttcgtc gagccgcacg agctgaccga cgcggaggtc cgcgcgaccg tcgccgactt
     2341 cgtcaccgcc gcccgcaacg ccgtggcggc cgggttcgac ggggtcgagc tgcacggcgc
     2401 caacggctac ctcatccacc agttcctcgc ccccaacacc aaccggcgca ccgacgcgtg
     2461 gggcggctcc gacgaggccc gcacccgctt cgccgtggag gtcgtcgagg ccgtcgccgc
     2521 cgagatcggc ccggagcgca ccggactgcg catctcgccc ggcaacccgt acaacgacat
     2581 cgacgagccc gcgccggagg ccacgtacac cgcgctggtc cgggcgatcg agccgctcgg
     2641 cctcgcctat ctgcacatcc tggaggccac cccgatccgc gagctgaccc tcgccctgcg
     2701 caaggccttc tccgggaccg tcgtcatcaa cgtgcactcc gacggacccg ccggccccgg
     2761 cgaccacacg gtgatcgacg acggcatcgc cgacctgatc tcctacgggg cactcttcct
     2821 cgccaacccg gacctgcccg cccggctgag ggcgggcggc ccgttcaaca cccccgaccc
     2881 gtcgaccttc ttcggcggcg acgccaaggg ctacaccgac tatccggcgc tcgacgccgt
     2941 ctgatcctcc aggggccgcc cgtcccaccg ccacgcggtg cggcgcgggt ggcccggcag
     3001 ttcggcgcgg ccggtggccc acagcaggac ggcccaccgg tcgccgcccg ccggagcgtc
     3061 cgggaagagc cgggccagcg cccggtcgca gagcgcggcg ggcggctccc acgtcagccc
     3121 cagcccctcc gcgatgtcat gggcgtgcac cagggtctcc accaccccca tcgcggcgaa
     3181 gccctccggg tccgagacgc tgtagacgtg gtacgcgcgc acctccggcg gcgtcgtccg
     3241 caccaccgag gccagcagcg ccccgcgcgc ctccaccgtc gccagcagcc cggcgggccc
     3301 ggccgagcgg tcgacgaaga gcaggccgtc gtggccgccc gggcgttcgg aggtccaccg
     3361 gaaggggagg ttctgctcca tcgggggcgt acgcgggccc aagcgcacgg cgtacgagac
     3421 gagatcgtcg cccaggtgct ccgccgtctc ccagcaggtc cactccagcg aaccggccct
     3481 ggcctcccag cggtccgccg gggccttcgc cagcacgtcc accgcgagcc gcacggcggt
     3541 cgtcacgtcg tcggcggtca ccggcagccg ggtcccggtc agcgccgccc gctcgtccgc
     3601 gccgcactcc acgcagaact cgttgccctc gggatcggcg agcgtcgccc agccgcgccc
     3661 gttcggtttg cggtggtcgg cgacgagggt cgcgccgagc gcgagcagcc gctcgacctc
     3721 ctcgtcgcgg gagcggtcgt ccggctggat gtcgagatgg acgcggttct tggtctgttt
     3781 cggctcgtcc acctgtacga agaggatcgc cgcgccgggc gcctcgacca gcgcctccgg
     3841 atcaccgggg gagtcctcgt cggagagcgg cgcaccgagc acctgggccc agaaggcggc
     3901 tagcgcgtag gcgtcggcgc agtcgatcgt gatgtggcgt acatgggaag tcatggcgga
     3961 caccctcccc gccgactcgg gtcccggtcc agtgagttac ccggcgaccg ggccggcttg
     4021 ccccccccgc gccccggtcc cggcggcaca cgcgaagggc cgggacggcg caccgtcccg
     4081 gcccttgccg cctgcgcgtc gcgctacctc accggggtga agtcccgcgc cccgatgaac
     4141 tccgggcggc ggatcggagc cgcgaagggc tcctgcgccg cgttctccac gctgttgaag
     4201 acgatgaaga cgttgctgcg cgcgtacggg gtgatgttgt cgcccgaacc gtgcatggcg
     4261 ttgcagtcga accaggtcgc cgaaccggcc ttgcccgtga agagcttgat gccgtggcgg
     4321 tcggccatct tcgtcagcgc ctcgtcggac ggggtgccgg cgtcctgcat ctggagcgac
     4381 ttcttgtagt tgtccttcgg cgtctcgccc gcacagccga ggaacgtctt gtgcgaaccg
     4441 ggcatgatca tcagcccgcc gttggtgtcg aagttctccg tcagcgcgat cgacacggac
     4501 accgtccgca tgttcggcag accgtcctcc gcgtgccacg tctcgaagtc cgagtgccag
     4561 tagaagcccg aggccccgaa gcccggcttc acgttgatcc gcgactggtg gacgtacacg
     4621 tccgagccga ggatctggcg ggcccggccc accacccgct cgtcgcggac cagaccggcg
     4681 aagacctcac tgatcttgtg gacctcgaag acggaccgca cgttctgcga ctgcttctcc
     4741 acgatggagc gctcgtcggc acggatcagc gggtcggcga ccagccggtc cagctccgcc
     4801 cggtagacgg cgacttcgtc cggcgtgatc agctgctcga tgctgaggaa gccgtcgtgc
     4861 tcgtagctct ggaggtcctt ggcggcgatc ggacccggtg cgcccggcgc ggaccagatg
     4921 accgggtcct ggcggggagt ggtcatctcg gcggcgccgc gcgaggggta cagatcggcg
     4981 cgtacttcgg tggtcatggt tcagccctcc tcggtcagca gtgggtagac accgttctcg
     5041 tcatggtcct cccgtccggt gacgggggga ttgaagacgc acacgcagcg gaagtcggtc
     5101 ttgggccgca tggtgtgccg ctcatgtccg tccagcagat acatcgtgcc gggcgtgatc
     5161 cagtgcttct cgccggtctc gtcgttggtg agctcggcct cgccctcggt gcacagcacc
     5221 gcctcgatgt ggttcgcgta ccacatggag gtctccgtgc ccgcgtacag cacggtctcg
     5281 tggagcgaga agcccacctt ctccttggcg agcacgatgc gcttgctctc ccaggtgccg
     5341 gacgcggcct tcacatgccg gtcggtgttc tcgatgtcac tgaacgatcg gacgatcacg
     5401 gtgagcggtt gcctttctct tctctgcacg ggccccgggc gtcgcggccc ggggcctctc
     5461 ttcttccacc tgccccggcg gttccgggtc aggccgtctc gcggacggag cgggccagag
     5521 tgcgcagccc ctcgtccagt tcctcggggg tgatggtcag cggcggcagc agcttgacga
     5581 cctcgctctg cgggccggag gtctccagca ggagccccag ctcgaaggcg cgggcgcaga
     5641 ccgccgaggc ccgcgccggg tcctcgaact ccatgcccca caccaggccc cggccgcgga
     5701 actgagcggt cggctcctcg gcgcagatgg ccagcagcgt ctgctcgacc tgctcgccgc
     5761 gggccagggt ctgcttctcc atctggccgt ccgcccagta cgcctggagc gcggcggcgg
     5821 ccgtgacgaa ggccgggttg ttgccgcgga aggtgccgtt gtgctcgccc ggctcccaga
     5881 tgtccagctc cggcttgaac aggcagagcg ccatcggcag cccgtagccg ctgatggact
     5941 tcgacagcgt gacgatgtcc gggacgatgc cggcctcctc gaaggagaag aagccgccgg
     6001 tccggccgca gcccatctgg atgtcgtcga cgatcagcag catgtcctgg cgctcgcaca
     6061 gctccttgag cgcgcgcagc cactcggcgc gggcgacgtt gatgccgccc tcgccctgga
     6121 ccgtctcgac gatcaccgcg gcgggcttgt tcagcccgga gccctggtcc tccagcagcc
     6181 gctcgaacca caggaagtcg gggaccgtac cgtcgaagta gttgtcgaac ggcatcggag
     6241 tgccgtgcac cagcgggatc ccggccccgg cgcgcttgaa ggcgttgccc gtgacggcga
     6301 gcgagcccag cgacatgccg tggaaggcgt tggtgaacga gacgacggac tcgcggccct
     6361 tgaccttacg ggccagcttc agcgccgcct cgacggcgtt ggtgccggtc gggccgggga
     6421 acatcacctt gtacggcagg tcgcgcgggc gcaggatcgc gttctggaac gtctccagga
     6481 acgcgcgctt ggcggtggtc gccatgtcca ggccgtgggt gatgccgtcg cgctcgatgt
     6541 agtcgatcag cgctcgtttc agcaccgggt tgttgtggcc gtagttgagc gagccggccc
     6601 cggcgaagaa gtccaggtag gagtggccgt cctcgtcggt cagccgggcg ccctgcgcac
     6661 ggtcgaagac ggcgggccag ccgcggcagt agctccggac ctcggactcc agggtctcga
     6721 agacactcag tgcgggcggg gtgatggtca cagcggatct cctgcgtgag ggggtgtgac
     6781 gggcggtggt ctcgtgctcc cggccgcacg ggtgcggccg ggaccgcgcc gggtggtcgg
     6841 ccggcgcgaa aagtcggggg ccgggcgcac gggtggggtg cgccgggcgg cgggcgtacg
     6901 ggccggggcg ccgagggcgc gcccgggcgg gctcaggtgg cgaacgggcc gatccggtag
     6961 aggacctcgg gcaggtgggt cccttcgggg aacaggtcga cgtcgaagag gacctcctgc
     7021 tccagcgcca cgtcgtgccg ctgggcgtag gacgtgaaca gccggtcgga ggcggtgttg
     7081 tccggggtga tcgtcgtctc gacggacgac aggccctggt cggcggcgac ccgggcggtc
     7141 agcgcgtcca gcagcgtggc ggccagtccc ttgccgcggt gggccgcgtc gacggccacc
     7201 tgccagacga cgagggtctg ggggcggtcg ggccggatgt agcccgtcac gaaggcgatg
     7261 ggctcgccgc tctcgccacg ggcgacgacg gaggtcgcgg cgaagtcacg gcaccacagc
     7321 aggtagctgt acgaggagtt caggtccagg acctgggagt cgcgggctat gcgccagatc
     7381 gcggctccgt cctccactcg cggcgcatcg atactgagga attcacttcg ggcacgggca
     7441 agatctgctg gtgcggcggt catgcgaatt gaatttaccc agcaaatttc gaaattgcat
     7501 cgacgtgagg ggttgggtga agagattgtg tgtgttatca ggcgtgcgcg agccgcccca
     7561 tgatcggggt ggaacttcca ccgctttgtc ctgaatttaa cgggcataac gccgctcgtg
     7621 tggagtcggt cacaggctca taactctcct ggagcgtgtc cgaaacatga agttcgagcc
     7681 ccgcgaaaac ttccgcgttt aggcgacgga aaagcgggca aaagaatgcg gggagctgct
     7741 ttcaataaag cagctccccg catggtggcc aaatcagcgg ctcggccagg tttccgaaac
     7801 ggcccggcgg gcagcctcga tatcgacctg ctggccccca tcggccagcg ccgcccccag
     7861 agccgcgagc gaggacagca ccacgccccg cgtcgcatcc gccccgtaat ggttgacccg
     7921 gatcatctcc ttggacagcg ccccgccgcc cgcgaccagc ggcagcgcgg ggtccgccgc
     7981 cagtgccgcc gcgaccagcg cggccgcgtc cgtccccgcc ggggcacgca gcgtcgtcgc
     8041 caccggggcc gcgtcccgtg cctcgtggac gtacggctcc agaccgccgc ccagcgccac
     8101 cgctcccgcc cgggtcgccg cggcggccga ggcgtggcgg gccatcaccg tctccaggcc
     8161 ctcgtcctcg atccgggcca cacacgcctc cagacccagc atctccagct gcgccggggc
     8221 gtgcggcagg gccgtgcggc cgccgtcgat ccagcgttcc ttccagtcca gcagcgagag
     8281 gtaggagcgg cgcggggcgg ccgggttggc ggcgatccgc tcccaggccc gcgcgctcac
     8341 cgacaccgcg gagaccccgg cgggcccgcc catggccttc tgcgcgccga tcacgcacag
     8401 gtcgacgccc caggcgtccg gcagcagcgg ttcggcgccc accgaggcga ccgcgtccag
     8461 catgaacagc gccccgtgcg ccccgaccac ctcgccgatc tccgcgaccg ggttggtgtt
     8521 gccggtcgcc gcctccgcgt gcaccagcga gacgaagtcg atctccgggt gcccggccag
     8581 cgcctcggcg acctggtcgg cggtcaccgc cgtgtggaag ggcaccgcga ggtcgacgac
     8641 ctgggcgccg cagtcccgca gccagttgcc gaaggtctgc ccgtacggac ccgtgaccac
     8701 gttcagcgcg gtcgagccgg gccgggcgcc gccgcggatg cagccctcca gcggcagcag
     8761 cgcctcgccc tgggtgatga cgacgtcctg ctccgtggcg aggagccggg ccacacgtcg
     8821 ctcgatcgcc gcgaagtggc cggcggtcag cgggggaagg tcgaggaaag ggtgcgtcac
     8881 ggtggtgctc tcttcgggtt cgtccgtcgg tcggaaggca ctggcagcct accgacggcc
     8941 caccggcggc ctcgtacagt gcggcgtatg agtgatcggg tgagcggtcg gacgggtggt
     9001 cggagtgagt gatcgcaagg agcggccggt gctgcgggtg aagggccggg tgctcgtcgg
     9061 cccggacgag gtgcgggacg aactgtgggc ggtggacgga cggatcacct tcgagcggcc
     9121 gcccggcgcg gacgacgcgg tgacggtcag gggctgggcg ctgcccggcc tcgtcgacgc
     9181 ccactgccat gtagggctcg accggcacgg cccggtcgac gcggcgaccg ccgagaagca
     9241 ggcgctcacc gaccgggagg ccgggactct gctgatccgc gacgccggat cgccctccga
     9301 cacccgctgg atcgacgacc gcgaggatct gccgaagatc atcagggccg gccgccacat
     9361 cgccaggacc cgccgctaca tccgcaacta cgcgcacgag atcgagcccg gcgacctcgt
     9421 cgcgtacgtc gcccaggagg cccggcgcgg cgacggctgg gtcaagctgg tgggggactg
     9481 gatcgaccgc gacgccgggg acctcaccgc ctgctggccg tgcggcgagg tcgaggcggc
     9541 catcgccgag gcgcaccggc tgggcgcccg ggtcaccgcg cactgcttcg ccgaggagtc
     9601 cctgcgcgat ctggtggagg cgggcatcga ctgcgtcgag cacgccaccg gcctcaccga
     9661 ggacaccatc ccgctcttcg ccgaacgcgg cgtcgcgatc gtcccgacgc tggtcaacat
     9721 cgccaccttc cccgacctgg cggcgggcgg cgaggccaag ttcccccgct ggtcggccca
     9781 tatgcggcag ctgtacgacc gccgctacga caccgtgcgc tccgcgtacg acgccgggat
     9841 cccggtctac gtcggcaccg acgcgggcgg ctccctggcg cacggcctgg tctcggggga
     9901 ggtggccgag ctggtgaagg cgggcatccc gccgctggag gccctctccg cgaccgcgtg
     9961 gggagccagg cgctggctgg ggcggcccgg cctcgacgag ggggctccgg cggacctggt
    10021 cgtgtacgac gcggaccccc gcgcggacgt ccgggtgctg gcggcgcccc ggcacatcgt
    10081 gctgaacggg cgggtcatcg gctgagtcgg cggtgcgttg acgggcagtg acgaatgcgg
    10141 cccccggctc gttgacatgg ccggcccgtc cacgcgccgc ggccccttcc gcgcgaaacg
    10201 ggtgacgggg aggggcggga gggaacgcac gtgccgaaag attcgaatgt atgcgcggaa
    10261 accccccttt ggggtgaact cacgtcaggt atccgccagt tcaccctcag tgcgtaaaga
    10321 ttcacggagt cgaggtcacc ggccgggcga tgtcccccat tggactgtgc cggtggctcc
    10381 atgtctcttg tgggggttc
//
