LOCUS       NZ_BMSZ01000011        11368 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces badius strain JCM 4350 sequence11, whole genome shotgun
            sequence.
ACCESSION   NZ_BMSZ01000011
VERSION     NZ_BMSZ01000011.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces badius
  ORGANISM  Streptomyces badius
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 265307)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 154X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649415.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:57:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,894
            CDSs (total)                      :: 6,825
            Genes (coding)                    :: 6,582
            CDSs (with protein)               :: 6,582
            Genes (RNA)                       :: 69
            rRNAs                             :: 2 (23S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 70 of 244
            Pseudo Genes (incomplete)         :: 206 of 244
            Pseudo Genes (internal stop)      :: 7 of 244
            Pseudo Genes (multiple problems)  :: 38 of 244
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:11:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 247960
            Orig. end                         :: 259328
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSZ01000011.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11368
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="RiPP-like"
                     /region_number="5"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11368
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11368
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="True"
                     /core_location="[5000:6368](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6368)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(1264..1280)
                     /note="TFBS match to HexR, Glucose-responsive regulator,
                     confidence: weak, score: 15.9"
                     /tool="antismash"
     misc_feature    complement(1266..1279)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 12.66"
                     /tool="antismash"
     gene            complement(1730..3469)
                     /locus_tag="IE135_RS20310"
                     /old_locus_tag="GCM10010253_41060"
     CDS             complement(1730..3469)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1288: ABC
                     transporter related protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955801.1"
                     /locus_tag="IE135_RS20310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_41060"
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="WP_199888743.1"
                     /transl_table=11
                     /translation="MMLHPELLRAARTARRPLGLATALLAAVAATHLAQAVLLALVLSR
                     IAQGETGALAPLLAAVIAVVLVRAALGRAQRLTAVTAGASVRVRLRDTLLERLGALGPT
                     EVTGARAGAVRATLVDGVEGVDAYLSRYLPQLLITAAVPPLLLVAVALVEPHAALALGP
                     ALLLALFGPRAWDRLLARRGKEHWDTYEALAADYLEALQGMPTLRNAGAVGRVRERLER
                     RSGALHRSTVAKLRVSLVDTGLTDLAVQGGTVAAVLVACSSAATGRTAATGTYLLLLLA
                     SECFRPVRDLSREWHAGYLGVSAADGIAALRTAEAAVPDRGTVTAAWGSAPDIRFEDVR
                     FTHPGGARPALDGVSFTAPAGRTTAVVGPSGAGKSTVLSLLLRQRDPDGGRVTVAGTDT
                     AAYTLASLRRGIAVVSQETYLFHATIAENLRIARPGATDEELRTAARTAGIDEEIARLP
                     DGYATLVGERGATLSGGQRQRLALARALIADAPVLILDEATSAVDERNQARIVRALAAA
                     GRGRTCVVVAHRLDAVRHADHIVVLDAGRVAAAGDHTTLLAGGGVYASLVAAGHTAQEE
                     RAA"
     gene            complement(3466..4986)
                     /locus_tag="IE135_RS20315"
                     /old_locus_tag="GCM10010253_41070"
     CDS             complement(3466..4986)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003964398.1"
                     /locus_tag="IE135_RS20315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_41070"
                     /product="nitroreductase"
                     /protein_id="WP_234427983.1"
                     /transl_table=11
                     /translation="MPHTRDRTAAPVRGADAALPAAPDLLRTSLADARSPRVPKLSPYA
                     PERPFPWSGPTTDTAGPTATNVDGPTATNAGERGTAIDLGRVLRLSLAAPLGPGGRLRP
                     VPSAGALHPVRAHLLLGPGCSLPPGRYTYDPRTHRAHRRGPAADRVPPGALVVLTVAAS
                     RTVAHYGHRAWPLLLLDAGHAAASLALAGATTAGVLVSLDADGSLLSAAAGLPEAPDWQ
                     RTWPGTEPELPLAAVRLTPRGGPCETEPPLGAWAALPRASAPTPQPGADTTPPRELAAA
                     RHLLHHIAEAPGRPGGTWHPASRPGEVTDEALETRRSAPPDDLKRPPASEPLAQILATA
                     RSVRPGGPSWTVAVGGAEPALYAAAPGGPGLDVRAVGDARPTLAHWAAGQQWIATSGAV
                     LLAHGCPANASRATVRGSHLVAGYAAGTAQAHAAALGLRSRPIGSWQQADLGAALGDTP
                     GRDWIVHGLALGAPPTTPATPATSAPTPATPPTASAAPAAPSGEEERP"
     gene            complement(5001..6368)
                     /locus_tag="IE135_RS20320"
                     /old_locus_tag="GCM10010253_41080"
     CDS             complement(5001..6368)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: YcaO"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059075.1"
                     /locus_tag="IE135_RS20320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_41080"
                     /product="YcaO-like family protein"
                     /protein_id="WP_199888763.1"
                     /sec_met_domain="YcaO (E-value: 4e-46, bitscore: 152.2,
                     seeds: 127, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTSALPLEALVDPVSGIVRAVAPVEHPAGAPPRYTAMTADVADAR
                     RLGAWPADRVSLGTTFGDPRGAWIAAVAEAVERYCGNRLPPLGHPQEPRRATAAELTAE
                     GARLYGPGALPTYAPWQYGRPRFPYGELTPDTPALWTRAVENGEPCWAPVALTHLNWRQ
                     GELRSLPRTHHLNYAGIATGQGLDDAVERGLLEVVERDALELWWHLDGPTRGIDPASVP
                     GLTDDLAGCGLDVHIVEMPSEFAPCVAALVHDPGRGMYAAGFACRYDPAEAARKAVLEA
                     VHTWVFTQGTVDADGWVYRAIEAGMLARGLYLDHRADRRYLDDCGPEFAAVRDLGAHVQ
                     VWLDARMTPLARRFTEPALGVVPVAAIAPGSRSALDAALAAGGHRVVTADLTTEDVAET
                     ALRVARVLVSGLIPNAPAAFGYFGCPRFAEAALARGWRTQRPTGPGDFTLAPPPHM"
     gene            complement(6365..7933)
                     /locus_tag="IE135_RS20325"
                     /old_locus_tag="GCM10010253_41090"
     CDS             complement(6365..7933)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377868.1"
                     /locus_tag="IE135_RS20325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_41090"
                     /product="CocE/NonD family hydrolase"
                     /protein_id="WP_199888745.1"
                     /transl_table=11
                     /translation="MNTRTVSVLTPDGTPLATDVCLPGGGRPRPAVLIRTPYGRDAHRA
                     ELRGWAAHGFAALAQDVRGRHGSPGEWHPYGDHEKADGAATVAWVRAQAWSNGEVVAVG
                     ASYAAYCALVTALDGPDGPRDAVAEKGLCDGVPDAVIAAVPALGLTETAREHGGPERLW
                     ARAGWWAAHGDRHDSDPDALGRALADAPRLLEHLPVAGLGERLAAGLGRELPSWPRLWA
                     DRERGRLVALGSAARLPLLAVGGTRDPFAEDTVALWRGWGGPARLLLGPWGHRLTADRS
                     APARARVNLGALYVRWARAALAGRLDPARHGVIALGDSGRWHSIHRAVSSSEAECRWPF
                     ASSTGLRLLHGAEFNADPERPVRSDDLAVPVDGGPADRCLLLSPPLPRPLDLAGAAVAR
                     INATADTPSADWAVRLTALDPSGRADPLAFGIVRRAAPPGEAADITVPLGTLGRRLPAG
                     TRLRAEIAGHHFPAHARNPHTGDDPVTATRLAPSRRTVNPRGSALHLPVVARRHYVEPV
                     PEICR"
     gene            complement(7930..8922)
                     /locus_tag="IE135_RS20330"
                     /old_locus_tag="GCM10010253_41100"
     CDS             complement(7930..8922)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010059073.1"
                     /locus_tag="IE135_RS20330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_41100"
                     /product="hypothetical protein"
                     /protein_id="WP_199888746.1"
                     /transl_table=11
                     /translation="MTRSRLAPGAGIVTVPGAAPVLRTSGGEFLRIDTGDVTGEALVRR
                     LAGEETPDGETPGKETPGEESQTAGLARLVEAFEAGGHAVSTPPRAPLAGRTVHVVGDP
                     VLTGPMARCAGAEGAEVHSITPDRVAELARTTRDPRTEGRVAVVWCLDSPVPEGLWDAA
                     DRLPDRRIAWLRCHREGSHSWTEPLAAAPGDVTSRHVRLRRLAATPAHRELAAYWAGHR
                     TPDTGPHPTEASAALIAALLTADLIAWAEAEAEAEAEAEAEAEATGGRAGGSGRQPAGV
                     PARRRLRRVDLRDLTFTEHAVLPVPDVAPLPAPTPAGTRARSGAAEARP"
     gene            complement(9057..9191)
                     /gene="amiA"
                     /locus_tag="IE135_RS35105"
                     /old_locus_tag="GCM10010253_41110"
     CDS             complement(9057..9191)
                     /codon_start=1
                     /gene="amiA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508296.1"
                     /locus_tag="IE135_RS35105"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_41110"
                     /product="streptamidine family RiPP"
                     /protein_id="WP_006129278.1"
                     /transl_table=11
                     /translation="MEQNKVFSPIADQGQLAHLSATHSNALVENPFDDAVEADTAAEK"
     misc_feature    9170..9184
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 16.28"
                     /tool="antismash"
     misc_feature    9259..9272
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.02"
                     /tool="antismash"
     misc_feature    complement(9259..9274)
                     /note="TFBS match to FuR_variant_1, Ferric Uptake
                     Regulator, confidence: weak, score: 18.12"
                     /tool="antismash"
     CDS             complement(9260..>9441)
                     /codon_start=2
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955796.1"
                     /locus_tag="IE135_RS34485"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="ABC transporter ATP-binding protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="REGRVAADGPPAEVVTRDLLAEVLGVEGRVGIDDEGGWPVCYPDH
                     PLEPLQLLRNENHIH"
     gene            complement(9260..>9443)
                     /locus_tag="IE135_RS34485"
                     /pseudo=""
     assembly_gap    9444..9477
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9478..10059)
                     /locus_tag="IE135_RS20335"
                     /old_locus_tag="GCM10010253_41120"
     CDS             complement(<9478..10059)
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /GO_function="GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955796.1"
                     /locus_tag="IE135_RS20335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_41120"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_234427984.1"
                     /transl_table=11
                     /translation="MSATESATPKAPDVVRAEGLAFGYPGRPVLRGVDLSIRAGELVAL
                     IGLNGCGKSTFLKLAAGLLAPSGGRVLLGGDDVARLPRRTAARRAALLHQSAPSVPGLT
                     VRQLVRQGRYAARGPLGMLREGDDEVTSRALADVGVTAWADRPVDALSGGERQRVRLAM
                     ALAQDAPVLFLDEPTTYLDLRHQLEVLQTVV"
     gene            complement(10089..11147)
                     /locus_tag="IE135_RS20340"
                     /old_locus_tag="GCM10010253_41130"
     CDS             complement(10089..11147)
                     /GO_function="GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1011: transport
                     system permease protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955794.1"
                     /locus_tag="IE135_RS20340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_41130"
                     /product="iron ABC transporter permease"
                     /protein_id="WP_199888747.1"
                     /transl_table=11
                     /translation="MTFVDTAPGPVRRTLRAPGVRVGGLALLVAVVACTGLFAGRGVTP
                     DGVRAVLLGAGGDPTAEHIVLQLRLPRVLVALVAGACLGVAGLVLQSALRNPLAGPEVT
                     GVTPGAVLGAVTATGLGLAGWESPAAVVVAACVGGFAGAGLLWLLTGRDRGDPEQTAVY
                     GVLVSAVLAGLTAVVLLVAPGELGSVVQWLIGSTEGRVWQHWNLLWPWAAFWGAAAWLL
                     AGPLTLLRCGDEQAAAAGLATGRGRGAALVCAVALTAGAVSAVGALGFVGLLVPHLALA
                     VFGADLRVTLPGAALLGAAVVCGADAVAQLLSRLLATALDADRLTLPVGALTTCVGAGL
                     LLVVARRRADER"
ORIGIN
        1 atgcggccgc cgtccaggac cgcgatccgg tcggccctgc ggacggtggc cgggcgatgg
       61 gcgatcacca gggtcgcccg ggtggcggag tcggcagcga gcgcccgggc cagttccgcg
      121 tcaccacggt ggtccaggtg ggcggtcgtc tcgtccagga cgaggacgcg cgggccgctc
      181 agcagccccc gggccagggc cacccgggcg cgttggccgc cggagagggt cgcgccgcgc
      241 tcaccgaccg cggtgtcgag tccgtcgggc agggtggcgg cgacggtgtc gacgccgcag
      301 atccgggcca ccgcgtccac ctcgtccgcc gtcgcgtcgg gggcgcccag gcggaggttc
      361 tcggcgaggg tgccgtggaa gagcggggcc tcctgcccga ccacggacac ggcggcgcgc
      421 aggtcgtcct cggcgaggtc ccgcaggtcc acggggtggg cgggggcgag gggcacgagt
      481 tccacggcac cctcggcggg gtcccagtag cgggccagca ggtgggcgca ggtcgacttg
      541 ccggccccgg acgctccgac gagggccacg gtctcccccg ggcggacggt gaggtcgacg
      601 ccgtccagca cggcccctcc tccgtagccg aaccgtacgc cgcgcagccg caggccgagc
      661 gggcccggcg ggacggggcg gggagcggtg gcggcggggg cgccggccgg ggcgtgcacg
      721 gcggcccgga cccgggccgc ggcggcgcgc aggccacccg cccggccgag cgcggccgcc
      781 gactcggaga ccggggccag tgcgcccagg gccagtgcca tggcggccgg ggcccacgcc
      841 ccgtcgagcc gtccggcgga cgcggcgtcg gcggcggccg ccacgacccc gatgacggcg
      901 gcgacgacca gcaggtcgcg gacagcgccc gcaccggtct cccacgactg ttcggcacgc
      961 tgggcgcggg ccagccgccg cccggcggcc cggagccgcg cccgcctgcg gttgagcgac
     1021 cccgtcatca ggagctcgcg gagtccgtcg acgctctcca cggtctccga cgacagctcg
     1081 gcgagcgcgc tccgggtgcg atgtccgcgt tcggcccgac cgcgcgcctc gatcagcggc
     1141 gaccagagga ggagcagcgc ggcgggtacg acggcgagca gcagccaggg gccgagggtc
     1201 gccagcacgg ccgccgacac cgcgaagacc acaccggagg cgaggagctg ggcgatggcg
     1261 tgggcgtaga agaattccag cgcctcgacg tccccgagcg cggtggccgc gaggtcgccg
     1321 ctgcgcctgc ccgcgatccg ggcgggggcg ctgcgggcca gtccgtcgaa gacccggacg
     1381 cgcagttcgg ccaggacccg gtaggcgagg tcgtgcgaga ggtccatctc gcgccaggtg
     1441 gccagcgccc ggaggaggac gaggccgatc aggacggcga cggtcccggg ggcgggcggg
     1501 cggtgctcga tgacagcggt gcccaccgtg tacgcggcga gggtgaccag ggccaccagg
     1561 gcggcctggt cgacgagggc ggccaggcag gtgcggagca cggtggcgcg gtgcgcgccg
     1621 aggaccggga ggagcgagcg cagggcaccg gtcggagccg ggtccgggac ctgggtcgtg
     1681 ggcggggcag ggggctgggg cgggtcctgg gtcgtgcgcg ggatcggggt catgcggcgc
     1741 gctcttcctg tgcggtgtgt ccggccgcga cgagcgaggc gtagacgccg ccgcccgcga
     1801 gcagggtggt gtggtcgccc gccgcggcca cacggcccgc gtccaggacg acgatgtggt
     1861 cggcgtggcg gacggcgtcg agccggtggg cgacgacgac gcaggtgcgg ccgcggccgg
     1921 ccgccgccag tgcccggacg atccgggcct ggttgcgttc gtcgacggcg ctggtggcct
     1981 cgtccaggat caggacgggg gcgtcggcaa tcaacgcccg tgccagggcg agccgttggc
     2041 gttgtccgcc ggagagcgtg gcgccgcgtt cgccgacgag ggtggcgtaa ccgtccggaa
     2101 gtcgggcgat ctcctcgtcg atcccggcgg tacgggcggc ggtgcgcagt tcttcgtccg
     2161 tggcgccggg gcgggcgatg cggaggttct ccgcgatcgt ggcgtggaag aggtaggtct
     2221 cctgcgacac cacggcgatg ccccggcgga gcgaggcgag ggtgtacgcg gcggtgtccg
     2281 tgccggctac ggtcacgcgc ccgccgtccg ggtcccgttg ccgcagcagg aggctcagca
     2341 cggtcgactt gcccgcgccg gatgggccga cgacggcggt ggtgcgcccg gcgggggcgg
     2401 tgaaggacac gccgtcgagg gcgggccggg cgccccccgg gtgggtgaag cgcacgtctt
     2461 cgaaacggat gtcgggcgca ctcccccagg ccgcggtcac ggtgccccgg tccgggacgg
     2521 cggcctcggc ggtgcgcagg gccgcgattc cgtcggccgc cgacaccccg aggtagccgg
     2581 cgtgccactc gcgggagagg tcgcggacgg ggcggaagca ttcggaggcg agcagcagca
     2641 gaaggtaggt gccggtggcg gcggtgcggc cggtggcggc cgaggagcac gccacgagaa
     2701 cggcggcgac cgtgccgccc tggacggcca ggtcggtgag gccggtgtcg acgagcgaga
     2761 cccgcagttt ggcgacggtg ctccggtgca gggcgcccga ccgccgctcc agccgttccc
     2821 gtacacggcc cacggcaccg gcgttgcgca gcgtcggcat gccctggagg gcttccaggt
     2881 agtcggcggc gagggcttcg taggtgtccc agtgctcctt gccccgccgc gccagcagtc
     2941 ggtcccacgc acgcggcccg aagagggcga gcagcagggc gggcccgagg gcgagcgccg
     3001 cgtgaggctc gacaagagcg acggcgacga gcagcagcgg cggcacggcg gcggtgatga
     3061 ggagttgggg cagatagcgc gagaggtacg cgtcgacgcc ctcgaccccg tccaccagcg
     3121 tggcccgtac ggcgccggcc cgcgcaccgg tgacctcagt ggggccgagc gccccgagcc
     3181 gctccagcag ggtgtcgcgc agcctcaccc gtacggaggc gccggcggtg accgcggtca
     3241 ggcgctgggc ccggccgagc gcggcgcgga ccaggacgac ggcgatcacg gccgcgagca
     3301 gtggggcgag ggcgcctgtc tccccctggg cgatccggga gagcacgagg gcgaggagga
     3361 cggcctgggc gaggtgggtg gcggcgaccg ccgcgaggag tgcggtggcg aggccgagcg
     3421 ggcggcgtgc ggtgcgcgcg gcgcgcagga gttcggggtg gagcatcatg ggcgttcttc
     3481 ctcgccggac ggggctgccg gggcggcgga tgccgtgggt ggggtggcgg gcgtgggggc
     3541 cgacgtggct ggggtggccg gtgtcgtcgg gggtgcgccg agcgcgagac cgtgcacgat
     3601 ccagtcgcgt ccgggggtgt cgccgagggc ggcgcccagg tcggcctgtt gccaggagcc
     3661 gatggggcgt gagcgcagac ccagtgcggc ggcgtgcgcc tgggccgtgc cggccgcgta
     3721 tccggcgacg aggtggctgc cgcggacggt ggcgcgcgat gcgtttgcgg ggcagccgtg
     3781 ggccaggagc accgcgcccg acgtcgcgat ccactgctgg cccgcggccc agtgggcgag
     3841 agtggggcgc gcgtcgccca cggcgcggac gtcgaggccc ggtccgccgg gagccgccgc
     3901 gtacagggcc ggttcggccc cgcccacggc cacggtccag gagggcccgc ccggccgcac
     3961 gctccgggcc gtggccagga tctgggcgag cggctcggag gcgggtgggc gcttcaggtc
     4021 gtcgggcggg gcgctccggc gcgtctccag agcttcgtcg gtcacctctc cggggcgcga
     4081 cgccgggtgc caggtgccgc cgggccgccc gggcgcttcg gcgatgtggt gcaacaggtg
     4141 ccgtgccgcg gcgagttcac ggggtggcgt ggtgtcggct cctggttgcg gagtcggggc
     4201 cgaggcgcgg ggcagcgcgg cccaggcgcc cagcggcggc tcggtctcac aggggccgcc
     4261 ccggggggtc aaccggactg ccgcgagggg tagttccggc tcggtgccgg gccaggtgcg
     4321 ctgccagtcc ggggcctcgg gcagtccggc ggcggcggac agcagggagc cgtccgcgtc
     4381 gagggagacc agcacaccgg cggtcgtggc cccggccagc gcgagggagg cggcggcatg
     4441 gcccgcgtcg aggagcagta gcggccaggc ccggtgaccg tagtgggcga cggtgcggga
     4501 cgcggccacg gtgaggacga cgagggcgcc cgggggcacg cggtcggcgg ccgggccgcg
     4561 ccggtgggcg cggtgggtgc gcgggtcgta ggtgtaacgc ccgggcggca gcgaacaccc
     4621 cggcccgagc agcaggtggg cgcgcaccgg gtgcagggcg cccgccgagg gaacggggcg
     4681 gagccggccc cctgggccca ggggcgcggc cagggagagc cgcagcaccc ggcccaggtc
     4741 gatcgcggtc ccgcgctcgc cggcgttcgt ggccgtgggg ccgtcgacgt tcgtggccgt
     4801 ggggccggcg gtgtcggtcg tggggccgct ccacgggaac gggcgttccg gagcgtacgg
     4861 ggagagcttt ggaacacgcg gcgaccgggc gtccgcgagc gacgtgcgca ggaggtccgg
     4921 tgcggcgggc agggcggcgt ccgccccgcg gacgggcgcg gcggtacggt cccgcgtgtg
     4981 gggcatgctg gcgtgctcca tcacatgtgc gggggcgggg cgagagtgaa gtcgcccgga
     5041 ccggtcgggc gttgggtgcg ccagccccgg gcgagcgccg cttccgcgaa gcgcgggcag
     5101 ccgaagtagc cgaaggccgc cggggcgttg gggatgagcc cggagaccag gacccgggcg
     5161 acgcgcagcg cggtctcggc gacgtcctcc gtggtgaggt ccgcggtgac gacccggtgg
     5221 cccccggcgg cgagcgccgc gtccagcgca ctgcggctgc cgggagcgat cgcggcgacc
     5281 gggacgacgc ccagggcggg ttcggtgaag cggcgggcga gcggggtcat ccgggcgtcc
     5341 agccacacct ggacatgggc gccgaggtcg cggacggcgg cgaactcggg cccgcagtca
     5401 tcgaggtagc gccggtcggc ccggtggtcg aggtagaggc cgcgggcgag catcccggcc
     5461 tcgatcgccc ggtacaccca cccgtcggcg tccaccgtgc cttgggtgaa cacccaggtg
     5521 tgcacggcct ccaggacggc cttgcgggcg gcctccgccg ggtcgtaccg gcaggcgaac
     5581 cccgccgcgt acataccgcg cccggggtcg tgcacgaggg ccgcgacgca gggggcgaac
     5641 tcggagggca tctccacgat gtgcacgtcc agcccgcagc cggccaggtc gtcggtgagc
     5701 ccgggtacgc tcgccgggtc gatgccgcgg gtgggcccgt ccaggtgcca ccacagctcc
     5761 agcgcgtcgc gctcgacgac ttcgaggagc ccgcgctcga cggcgtcgtc gaggccctgc
     5821 ccggtggcga tgccggcgta gttgaggtgg tgggtgcggg gcagtgaacg cagctccccc
     5881 tgacgccagt tgaggtgggt gagcgcgacg ggggcccagc agggttcccc gttctcgacc
     5941 gcccgggtcc acagtgccgg tgtgtcgggg gtgagttcgc cgtacgggaa gcgagggcgg
     6001 ccgtactgcc acggtgcgta cgtcggcaga gcgccggggc cgtagaggcg ggcgccctcg
     6061 gcggtcagct cggccgcggt ggcccgccgg ggctcctgag ggtggccgag cggcgggagg
     6121 cggttgccgc agtagcgctc cacggcctcg gccaccgcgg cgatccaggc gccccggggg
     6181 tcgccgaacg tggtgccgag cgagacccgg tcggcgggcc aggcgcccag gcggcgggcg
     6241 tcggcgacgt cggcggtcat cgcggtgtag cggggcggcg caccggcggg gtgttcgacg
     6301 ggtgcgacgg cgcggacgat cccgctgacg gggtcgacga gggcttcgag gggcagggcg
     6361 ctggtcaacg gcatatctcc ggtacgggct cgacgtagtg gcgccgggcg acgacgggca
     6421 ggtggagggc gctgccccgg gggttcacgg tgcggcggga cggggcgagg cgggtggccg
     6481 tgaccgggtc gtcgccggtg tgcgggttgc gggcgtgggc ggggaagtgg tgtccggcga
     6541 tctcggcccg caaccgggta ccggcgggca gcctgcggcc cagggtgccg agcggcacgg
     6601 tgatgtccgc ggcttcgccg ggcggggcgg cgcgccggac gatgccgaag gcgagcggat
     6661 cggcccggcc ggacgggtcg agcgcggtca ggcgcacggc ccagtcggcc gacggtgtgt
     6721 cggcggtggc gttgatccgg gctacggcgg cccccgccag gtcgaggggg cggggcagcg
     6781 gcggcgagag cagcagacag cggtcggccg ggccgccgtc gaccgggacg gcgaggtcgt
     6841 cggagcgcac ggggcgctcc gggtcggcgt tgaactcggc tccgtggagc agccgtagac
     6901 cggtggacga cgcgaagggc cagcggcact cggcctcgga ggaggacacc gcccggtgaa
     6961 tgctgtgcca acggccgctg tcgccgaggg cgatgacccc gtggcgggcc ggatccagtc
     7021 gcccggcgag cgcggcgcgg gcccatcgta cgtacagggc accgaggttg acacgcgcgc
     7081 gggcgggcgc ggaccggtcg gcggtgaggc ggtgccccca ggggccgagc agcagacgcg
     7141 ccggtccgcc ccagccgcgc cagagggcca cggtgtcctc ggcgaagggg tcgcgagtgc
     7201 caccgacggc gagcagcgga aggcgggccg cggagccgag cgccacgagg cggccgcgct
     7261 cccggtcggc ccagagccgg ggccaggaag gcagttcacg cccgagaccc gcggccagcc
     7321 gctcccccag gccggcgacc gggaggtgtt cgagcagccg gggggcgtcg gccagcgcgc
     7381 gccccagcgc gtccgggtcg gagtcgtgcc ggtcgccgtg ggccgcccac cagccggccc
     7441 gggcccacag ccgctccggc cccccgtgct cgcgcgcggt ctcggtgagc ccgagcgcgg
     7501 gaacggcggc gatgacggcg tcggggactc cgtcgcaaag ccccttctcg gcaacggcgt
     7561 cccgggggcc gtcggggccg tcgagtgcgg tgacgagggc gcagtacgcc gcgtaggagg
     7621 cgccgacggc gacgacctct ccgttgctcc acgcctgtgc gcgcacccag gcgaccgtgg
     7681 ccgctccgtc cgccttctcg tggtccccgt acgggtgcca ctcccccggt gagccgtggc
     7741 ggccgcgtac gtcctgggcg agggcggcga atccgtgtgc cgcccagcca cgcagctcgg
     7801 cacggtgggc atcgcgcccg tacggggtgc ggatcaggac ggcggggcgg gggcggcccc
     7861 cgcccgggag gcatacgtcg gtggcgagcg gggtgccgtc cggcgtgagg acggacacgg
     7921 tacgggtgtt cacgggcggg cctcggcggc gccggaccgt gcgcgcgtcc ccgccggtgt
     7981 cggcgcgggc agtggcgcga cgtccgggac ggggaggacg gcgtgttcgg tgaaggtgag
     8041 atcgcgcaga tcgacccggc gcagccggcg ccgggccggg acaccggccg gctgccgacc
     8101 cgaaccgccc gcgcgcccgc ccgttgcttc ggcttcggct tcggcttcgg cttcggcttc
     8161 ggcttcggct tcggcccagg cgatcagatc ggcggtgagc agcgcggcga tcagggcggc
     8221 ggatgcctcg gtggggtgcg gtccggtgtc gggggtgcgg tgcccggccc agtacgcggc
     8281 gagttcgcga tgggcggggg tggccgcgag cctgcggagt cggacatgcc gggaggtcac
     8341 gtctcccggg gccgcggcaa ggggctcggt ccagctgtgg gagccctcgc ggtggcagcg
     8401 cagccaggcg atacgtcgat ccggcagccg gtccgccgcg tcccagagcc cttcggggac
     8461 gggcgagtcc aggcaccaca ccacggcgac gcggccttcg gtgcgggggt cccgggtcgt
     8521 ccgcgcgagt tccgccaccc ggtccggggt gatgctgtgc acctcggccc cttccgcccc
     8581 ggcgcagcgc gccatgggcc cggtgagcac ggggtcgccg acgacatgga cggtgcggcc
     8641 ggcgagcggg gcccggggcg gggtggacac ggcgtgccca ccggcctcga acgcctcgac
     8701 gagccgggcg agtccggcgg tctgcgactc ttcgcccggc gtctccttgc cgggcgtctc
     8761 cccgtccggc gtctcctcgc ccgccagccg gcgcaccagg gcttccccgg tgacgtctcc
     8821 ggtgtcgatg cgcaggaact ctcctccgga ggtccggagc acgggggcgg cgccgggaac
     8881 ggtgacgatc ccggcaccgg gagcgagtcg actgcgggtc acggaatcct ccgggtcgga
     8941 gagagggacg gcgggacggg gtgatcgggc cgaacggggc gggcccgccc gggttgtcac
     9001 cgggcgggcc cgcccccgcg ccatgcggga gacctgcctt cagcggcagg cgtcgcttac
     9061 ttctcggccg cggtgtcggc ctcgacggcg tcgtcgaagg ggttctcgac gagggcgttc
     9121 gagtgcgtgg cggagaggtg ggcgagctgg ccctggtcgg cgatcgggct gaacaccttg
     9181 ttctgctcca tgagttgaag cctccattca catcggatgg gtgtgggtcg cggcgttggt
     9241 gcgagccgca gcacagacat taatgaatat gattttcatt ccgcaatagc tggagcggtt
     9301 cgagagggtg atctgggtaa cagacgggcc agccgccctc gtcgtcgatc cccacccggc
     9361 cttccacgcc caggacttcg gccagcagat cccgggtcac gacctcggcc gggggcccgt
     9421 cggcggcgac gcggccttcc cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnntac
     9481 gaccgtctgg agcacctcca gctggtgccg tagatccaga taggtggtcg gctcatcgag
     9541 gaacagcacg ggggcgtcct gagccagcgc catggccagc cggacacgct ggcgttcacc
     9601 tccggacagc gcgtccacgg gccggtcggc ccaggccgtg acaccgacgt ccgcgagcgc
     9661 ccgggacgtc acctcgtcat caccctcccg cagcatcccg agcggccccc gcgcggcata
     9721 acgcccctgg cgcacgagtt gacgtaccgt cagccccggt acggaaggtg ccgattggtg
     9781 cagcagtgcc gcacgccggg cggcggtgcg gcggggcagc cgtgccacgt cgtcgccgcc
     9841 gagcagcaca cgcccgccac tcggggcgag cagcccggcg gcgagcttga ggaaggtgct
     9901 cttgccgcag ccgttcagcc cgatcagggc gaccagttca ccggcccgga tactcaggtc
     9961 caccccgcgc agcaccgggc gtccggggta gccgaacgcc agtccctccg cccggacgac
    10021 gtccggggct ttcggggtcg cgctctccgt cgcgctcatc tccactgtcc tctccctcgt
    10081 tctctccctc atctctcgtc cgcccgtcgg cgtgccacga ccagcagcag ccccgccccc
    10141 acacaggtgg tcagggcgcc gaccgggagg gtcagccggt ccgcgtcgag cgcggtggcc
    10201 agcagccggg acagcagttg ggccacggcg tccgccccgc acaccaccgc cgcaccgagc
    10261 agcgcggcgc ccgggagcgt gacgcgcaga tcggcgccga agacggccag tgccagatgg
    10321 ggcaccagca gcccgacgaa cccgagggcg cccaccgccg acaccgcgcc ggccgtcagc
    10381 gccaccgcgc acacgagcgc cgcccccctg ccccggccgg tggccaggcc cgcggcggcg
    10441 gcctgttcgt caccgcagcg cagcagggtc agcggcccgg cgagcagcca ggccgccgca
    10501 ccccagaagg cggcccacgg ccagagcaga ttccagtgct gccagacccg gccctcggtg
    10561 gagccgatca accactggac gacgctgccg agttcaccgg gagccaccag gaggacgacc
    10621 gcggtgagtc cggcgagcac ggccgacaca aggactccgt agacggcggt ctgttccggg
    10681 tcgccccggt cccgcccggt gagcagccac agcagtcccg ccccggcaaa gccgccgacg
    10741 caggccgcca cgaccacggc cgccggggac tcccaccccg cgagaccgag gccggtcgcg
    10801 gtcaccgcac cgagcaccgc gccgggggtg acaccggtga cctcgggccc ggccagcggg
    10861 ttgcgcaagg ccgactggag cacgagtccg gcaacaccga ggcaggcccc ggccaccagg
    10921 gccaccaaca cgcggggcaa gcggagttgg agcacgatgt gctcggcggt ggggtcgccg
    10981 ccggccccga gcagcacggc ccgcacgccg tcgggggtga cgccccgacc ggcgaagagt
    11041 ccggtgcagg cgacgactgc gacgagcagc gccaggccgc ccacccggac gcccggggcg
    11101 cgcagggtcc ggcggaccgg tccgggcgcg gtgtcgacga aggtcatcgt tccgccttcc
    11161 gcaggacggg agcgagggcg tccggcgaag tcaccggagc ggcgagggag gcaggccccc
    11221 ggtcccgggc gggccgccgc ccccgtttca gcagcacgac cccgaccggc acgccgacga
    11281 gggcggtcca ggcgccgacg ggcgtctcga ccggtgcgag ggcgagccgg gccggctggt
    11341 cggcgcagag gacgacgatc gcgccgca
//
