LOCUS       NZ_BMSZ01000006        15210 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces badius strain JCM 4350 sequence06, whole genome shotgun
            sequence.
ACCESSION   NZ_BMSZ01000006
VERSION     NZ_BMSZ01000006.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces badius
  ORGANISM  Streptomyces badius
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 358496)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 154X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649415.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:57:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,894
            CDSs (total)                      :: 6,825
            Genes (coding)                    :: 6,582
            CDSs (with protein)               :: 6,582
            Genes (RNA)                       :: 69
            rRNAs                             :: 2 (23S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 70 of 244
            Pseudo Genes (incomplete)         :: 206 of 244
            Pseudo Genes (internal stop)      :: 7 of 244
            Pseudo Genes (multiple problems)  :: 38 of 244
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:11:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 111918
            Orig. end                         :: 127128
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSZ01000006.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..15210
                     /candidate_cluster_numbers="1"
                     /candidate_cluster_numbers="2"
                     /candidate_cluster_numbers="3"
                     /contig_edge="False"
                     /product="ectoine"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..15210
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /detection_rules="AfsA"
                     /kind="neighbouring"
                     /product="ectoine"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /protoclusters="2"
                     /tool="antismash"
     cand_cluster    1..10375
                     /candidate_cluster_number="2"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10375
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5375](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5375
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    198..207
                     /note="TFBS match to AbrC3, Antibiotic production
                     activator, confidence: strong, score: 18.16"
                     /tool="antismash"
     gene            complement(478..1095)
                     /locus_tag="IE135_RS12200"
                     /old_locus_tag="GCM10010253_24750"
     CDS             complement(478..1095)
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763163.1"
                     /locus_tag="IE135_RS12200"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24750"
                     /product="TetR family transcriptional regulator"
                     /protein_id="WP_199888145.1"
                     /transl_table=11
                     /translation="MQERAARTRQAVLEAAAEEFAERGYEGASLQRVACRAETSIGALT
                     FHFRNKTALAEEVRLTGRARFDRSLAGLVPAADPLRELRVLIGTLARLAREDRFVRAVR
                     RLEAGPPDSAPPLAETWMPVLRAVLDRAHGARRLRPGVAPEEAEAMLAHLAEGVMAAPG
                     TPRSAAGGAVWDSVWDVVLHGLAADRGEGSVRDDREPSRAES"
     misc_feature    complement(856..858)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            1217..2326
                     /locus_tag="IE135_RS12205"
                     /old_locus_tag="GCM10010253_24760"
     CDS             1217..2326
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018961775.1"
                     /locus_tag="IE135_RS12205"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24760"
                     /product="transposase"
                     /protein_id="WP_234427859.1"
                     /transl_table=11
                     /translation="MHETPEMATGRALDDLSRLLFGHLHRSDQQRWGHVYLQGLLRTHG
                     KKTVRRMAATVTGSEATSSSLHQFVNASPWEWAPVRGRLARWATRSLPVRSWTLAPTVL
                     PKRGNHSPGVHQRFLPESGRTVNCQLGLGLFLNTGQAHLPVDWQLFLPPRFTQDPAVRD
                     RAKIPATARPQTPGDHALQLVQEMTSWTDGETAPVVADLTALCDTPQLARRLQGSGHSF
                     VLAVPGQTAIGAAELPPGERPSARALLLRRGVRHPAGAGRRIISYETPVPGLRPSGEST
                     HGPGRLLAEWDADAGRPGRIWLTNITDRHPAELLELAHSPRGAESAVRRMGEDFGLLDF
                     EGRSFPGWHRHMTLVSAAYTCATLEGMNP"
     misc_feature    1325..1327
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    2398..2411
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.74"
                     /tool="antismash"
     gene            2449..2892
                     /locus_tag="IE135_RS12210"
                     /old_locus_tag="GCM10010253_24770"
     CDS             2449..2892
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1135: MarR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489542.1"
                     /locus_tag="IE135_RS12210"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24770"
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="WP_069735571.1"
                     /transl_table=11
                     /translation="MTINQQPRAASDQTMWEAVLGLHAFVERQLAHTLQRRYGVGLSEY
                     RALETLHQAEKGECRMQELADHIGLGQSSVTRLVGRLDSAGYAYKDSCADDKRGVFAVI
                     TEEGRKRYQEARATYADVLSSALNTASTDAQLARAVQALRSAA"
     misc_feature    complement(2986..3001)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 16.03"
                     /tool="antismash"
     misc_feature    complement(3019..3032)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.22"
                     /tool="antismash"
     gene            3203..4651
                     /locus_tag="IE135_RS12215"
                     /old_locus_tag="GCM10010253_24780"
     CDS             3203..4651
                     /NRPS_PKS="Domain: AMP-binding (1-382). E-value: 1.1e-57.
                     Score: 187.4. Matches aSDomain:
                     nrpspksdomains_IE135_RS12215_AMP-binding.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) AMP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1002: AMP-dependent synthetase and ligase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489543.1"
                     /locus_tag="IE135_RS12215"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24780"
                     /product="AMP-binding protein"
                     /protein_id="WP_079133915.1"
                     /sec_met_domain="AMP-binding (E-value: 2.2e-59, bitscore:
                     195.5, seeds: 400, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQEQPDSTALVAAGERVSYAELERLTRRATADLDGAGFGSVRALC
                     VPAHKSPETIALLLAAWRQGINTLVPSPGLGAAALATLTAQAHGPLTATAVPGGGVSLV
                     REETDPAAADGFSVPDPAESLLTLTTSGSTGVPKLVPIRTHAFDRFADWATGKFGIDGT
                     TRALSFSPLNFDLALLDVWTVLEAGGTVVLADAGASGDAGYLRELTLAHEVTLVQGVPL
                     LHRLLAAEGARYPAVRTVVFTGEAVSEQGLRDAFASCPGARFHNVFGCTETNDSFIEDI
                     DPDAYVHPLPIGTPVDGTDAVLLIDGPEGSEVLQGPGTGELLVHTPFQTCGYLEQARNA
                     RAFMPDPRGGGAMFYRTGDLVHRDADGRYFLQGRTDFQVKVRGVRTNTMEVETVLGAHP
                     DVVEACVVALPCPEAGNRLHAEVVRRDGASLSSLGLRAYAAKHLPRHAVPSSVGLAGTP
                     LPKTPTGKPDRNSIKKNRLNGDTS"
     aSDomain        3206..4348
                     /aSDomain="AMP-binding"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE135_RS12215_AMP-binding.1"
                     /evalue="1.10E-57"
                     /label="IE135_RS12215_AMP-binding.1"
                     /locus_tag="IE135_RS12215"
                     /protein_end="382"
                     /protein_start="1"
                     /score="187.4"
                     /specificity="substrate consensus: Ala"
                     /tool="antismash"
                     /translation="QEQPDSTALVAAGERVSYAELERLTRRATADLDGAGFGSVRALCV
                     PAHKSPETIALLLAAWRQGINTLVPSPGLGAAALATLTAQAHGPLTATAVPGGGVSLVR
                     EETDPAAADGFSVPDPAESLLTLTTSGSTGVPKLVPIRTHAFDRFADWATGKFGIDGTT
                     RALSFSPLNFDLALLDVWTVLEAGGTVVLADAGASGDAGYLRELTLAHEVTLVQGVPLL
                     HRLLAAEGARYPAVRTVVFTGEAVSEQGLRDAFASCPGARFHNVFGCTETNDSFIEDID
                     PDAYVHPLPIGTPVDGTDAVLLIDGPEGSEVLQGPGTGELLVHTPFQTCGYLEQARNAR
                     AFMPDPRGGGAMFYRTGDLVHRDADGRYFLQGRTDFQVKVR"
     CDS_motif       3584..3628
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS12215_0001"
                     /evalue="4.90E-04"
                     /label="NRPS-A_a3"
                     /locus_tag="IE135_RS12215"
                     /protein_end="142"
                     /protein_start="127"
                     /score="13.1"
                     /tool="antismash"
                     /translation="LTTSGSTGVPKLVPI"
     cand_cluster    4278..15210
                     /candidate_cluster_number="3"
                     /contig_edge="False"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="2"
                     /tool="antismash"
     protocluster    4278..15210
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[9277:10210](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="2"
                     /tool="antismash"
     proto_core      complement(9278..10210)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="2"
     CDS_motif       4319..4378
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS12215_0002"
                     /evalue="3.90E-06"
                     /label="NRPS-A_a8"
                     /locus_tag="IE135_RS12215"
                     /protein_end="392"
                     /protein_start="372"
                     /score="19.4"
                     /tool="antismash"
                     /translation="GRTDFQVKVRGVRTNTMEVE"
     gene            4648..4908
                     /locus_tag="IE135_RS12220"
                     /old_locus_tag="GCM10010253_24790"
     CDS             4648..4908
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763164.1"
                     /locus_tag="IE135_RS12220"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24790"
                     /product="acyl carrier protein"
                     /protein_id="WP_069735573.1"
                     /sec_met_domain="PP-binding (E-value: 4.3e-07, bitscore:
                     24.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTTASDPIRAYIVSEFLAGEDAEDLTDDFDLIESTVVNSLGLVR
                     LISWISETYRIPVDDIPLEPAAYRTLADIRRFVEQAAPASV"
     gene            5001..5375
                     /locus_tag="IE135_RS12225"
                     /old_locus_tag="GCM10010253_24800"
     CDS             5001..5375
                     /GO_function="GO:0033990 - ectoine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452967.1"
                     /locus_tag="IE135_RS12225"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24800"
                     /product="ectoine synthase"
                     /protein_id="WP_069735574.1"
                     /sec_met_domain="ectoine_synt (E-value: 8.8e-26, bitscore:
                     85.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSLSDVPAVEWGSGTSHRLLTEADGMGFTVCETLVRKGTSSG
                     LQYLGHLEACYCIGGSGEVVTTEGVRHKIKTGTIYALDQHDAHHLLASDDEDLLLVSVF
                     SPALRGDEKHSLSEDGFSRY"
     assembly_gap    5390..5426
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5427..6537
                     /locus_tag="IE135_RS12230"
                     /old_locus_tag="GCM10010253_24810"
     CDS             <5429..6537
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_N"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_M"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Acyl-CoA_dh_1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1006: acyl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489546.1"
                     /locus_tag="IE135_RS12230"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24810"
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="WP_234427860.1"
                     /sec_met_domain="Acyl-CoA_dh_N (E-value: 6.6e-20, bitscore:
                     66.4, seeds: 790, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_M (E-value: 7.2e-27, bitscore:
                     88.0, seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="Acyl-CoA_dh_1 (E-value: 2.9e-33, bitscore:
                     109.6, seeds: 49, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DTYGVTAPYGDELNDDVARWDRAGEFPREKWKPLQRSGLFTLPFA
                     PEYGGAGRTLTETMSALEGLGHTSRDAGLNFSASTQIVSVGIPLQAFGSEELRSRYLPR
                     VTSGEAITAHAITEPSHGSDVMNIRTTAVREGDEYVIDGGKMFITNAPVADLFLLYVRT
                     GKPGAFGLSTFLAERSTPGLKIGEPLETMGLRTSPISEVTLENVRLPAANRLGSEGAGF
                     LIMDYVMKREVLFSFAVNLGEMTHRLKRVVEHATTRQQFGSAIGKNQAVSHKIADMRIA
                     VETARKWLYDTGRKVEQGTDASLDVAATKIVVSEANQLTAQHAIQIFGARGYLTDTGIE
                     RELRNATSGSIYSGTNEIQRNCIAALMGL"
     gene            6553..7272
                     /locus_tag="IE135_RS12235"
                     /old_locus_tag="GCM10010253_24820"
     CDS             6553..7272
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489547.1"
                     /locus_tag="IE135_RS12235"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24820"
                     /product="transglutaminase family protein"
                     /protein_id="WP_176734495.1"
                     /transl_table=11
                     /translation="MTDTLTDTVSAPPAPEDLDRLTAATDFLDHEHGTVRAFVDKALDG
                     IDREKAGQVELAVALYYAVRDGIHYEVYGADLSPEGLKASSIITGGKGFCLHKSVLYAA
                     ACRSVGIPARLHYSDVRNHLASDRLRSYIGGDVFFHGLVTVYLEGRWLQVTPVFNKLLC
                     RLYGMTPLEFDGRSDSLYHPFDAQGRQSMEFLTDHGDFDDVPYEFVLDNMRRKHPKFLD
                     ADGTGTVSGGSLADEAN"
     gene            7332..8147
                     /locus_tag="IE135_RS12240"
                     /old_locus_tag="GCM10010253_24830"
     CDS             7332..8147
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763169.1"
                     /locus_tag="IE135_RS12240"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24830"
                     /product="maleate cis-trans isomerase"
                     /protein_id="WP_069740806.1"
                     /transl_table=11
                     /translation="MTQFQAREDGWGAVARVGVVVPHADVGPESELQAMAPRDVYIHGS
                     RVHFGAMRPGGEMDPKIPHDPVRAFIDPPFIDDAVELLAASPLDSIALGFTSSAYVLGA
                     DGEQKLFERLAERTRGIPLTGTTHAATAGFRALGAERIALVNPPWFDDELSALGASYFG
                     ERGFDVVHHAPCGLPSNQKLITPEALVQWIESTVAPQKPQAVLVAGNGIRAVGTIAGLE
                     ERIGFPVLTANQVLFWHALHLAGGTEAAARISDYGALFGVRPEPGSVTG"
     gene            8144..8386
                     /locus_tag="IE135_RS12245"
                     /old_locus_tag="GCM10010253_24840"
     CDS             8144..8386
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489549.1"
                     /locus_tag="IE135_RS12245"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24840"
                     /product="DUF6253 family protein"
                     /protein_id="WP_019763170.1"
                     /transl_table=11
                     /translation="MSLLPAYGYQAAVQETCDTPEYHMPLVCWHDDDNGVRGLVLYRGA
                     LRPAEQVPGFRRYLAAEGLEQYATTGPWPLLAATA"
     gene            8442..9194
                     /locus_tag="IE135_RS12250"
                     /old_locus_tag="GCM10010253_24850"
     CDS             8442..9194
                     /NRPS_PKS="Domain: ACPS (132-191). E-value: 1e-07. Score:
                     23.7. Matches aSDomain:
                     nrpspksdomains_IE135_RS12250_ACPS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1012: 4'-phosphopantetheinyl transferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763171.1"
                     /locus_tag="IE135_RS12250"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24850"
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="WP_069735577.1"
                     /transl_table=11
                     /translation="MTHDLKWSDAPPGEVRDQVGVWIVRAPAGQRLPPDGSPPGWLDEK
                     ERSRLAAIPQRSGKEAYAFAHWALRSVLGSSLGCPPRGVAFLRQPCPGCGGAHGRPAIR
                     DPDRGGEPPEFSMSHSGDHVAIAVAEATIGIDIECHPTPESVAGYLPFLHPREQQWLGG
                     RPADELVRDFARLWTRKEAMAKATGQGMAAVMNRLDLTGQPLGWRVRQLIAPPGYEASF
                     AVPASVHVDVIMYEDLPHPMPEAELEPV"
     aSDomain        8838..9014
                     /aSDomain="ACPS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE135_RS12250_ACPS.1"
                     /evalue="1.00E-07"
                     /label="IE135_RS12250_ACPS.1"
                     /locus_tag="IE135_RS12250"
                     /protein_end="191"
                     /protein_start="132"
                     /score="23.7"
                     /tool="antismash"
                     /translation="IGIDIECHPTPESVAGYLPFLHPREQQWLGGRPADELVRDFARLW
                     TRKEAMAKATGQGM"
     gene            complement(9278..10210)
                     /locus_tag="IE135_RS12255"
                     /old_locus_tag="GCM10010253_24860"
     CDS             complement(9278..10210)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489551.1"
                     /locus_tag="IE135_RS12255"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24860"
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_069735578.1"
                     /sec_met_domain="AfsA (E-value: 1.2e-23, bitscore: 77.5,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVSVLSRDVQPVQQSQVHQWQVHKLRGEEVLLRTWTEEDSGRFV
                     ITARWPKGHAFYQPSQGAYDPLLLAETVRQTVPLLSHAVHGVPREHKQAWECLDFAVEP
                     LAALVTTAEDEVRLVVSCSDVVRRGNRFAGMTMDIDVHLGKRLMGIAHTRFNDQPAAVY
                     RRMRRGYADIDAALENRLPAGPPVEPHRVGRGSPADVVLSPALGGERPGRWLLRVDTAH
                     PVLFDHPVDHAPGMLLLEAARQAARAVGGATAAVIGMRTNFVRYVELDAPAVVVAGSPA
                     PVGPGRRRVPVSIEQDGVQVFTSEVTIAG"
     misc_feature    complement(9917..9919)
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    complement(10286..10295)
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 15.04"
                     /tool="antismash"
     misc_feature    complement(10445..10455)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.59"
                     /tool="antismash"
     gene            10455..11087
                     /locus_tag="IE135_RS12260"
                     /old_locus_tag="GCM10010253_24870"
     CDS             10455..11087
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1057: TetR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018489552.1"
                     /locus_tag="IE135_RS12260"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24870"
                     /product="ScbR family autoregulator-binding transcription
                     factor"
                     /protein_id="WP_069735579.1"
                     /transl_table=11
                     /translation="MAQPKQERAEQTRRALLHAAAEVFDEFGYAGASITRILKRAGVTA
                     GALYFHFGSKEDLAKAVMNSQPETLVPLVAAGGLQRLIDITLVWSWQLQRDPLLRAGVR
                     LTNEQTSVGGPANADPYEKFRAIMTGCLQDAQNDGELQPGVDPVVVAEFVVAACTGMQM
                     YSNVVSGRQDLPERTQQMWNLILPGIAVPSVITRADATPARGAAMMP"
     gene            11141..11860
                     /locus_tag="IE135_RS12265"
                     /old_locus_tag="GCM10010253_24880"
     CDS             11141..11860
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763173.1"
                     /locus_tag="IE135_RS12265"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24880"
                     /product="HAD family phosphatase"
                     /protein_id="WP_199888146.1"
                     /transl_table=11
                     /translation="MLSLRLAALNIDGVLLNDTFSPVIHQFVVSRGGTYSAALERSVFS
                     QSQHIAGQRLAAAVGGGMTGEAALAAYFEERAAHVGRHPVTITPGTHRLLLRLRSAGLH
                     TVCYGGLGKEHFDRHLGRYAHLFDGPGYVSTNDIRPGLREITEDVFGLRPAQALFVDDV
                     ARVAEEARALGTAFVGHPSAFAHSHQRTLMREAGVRHMVDRLDALDDGLLHRIDQEMST
                     GTFWSPALVAAGRERQA"
     misc_feature    11150..11152
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            11857..12627
                     /locus_tag="IE135_RS12270"
                     /old_locus_tag="GCM10010253_24890"
     CDS             11857..12627
                     /EC_number="1.1.1.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /NRPS_PKS="Domain: PKS_KR (11-181). E-value: 4.6e-13.
                     Score: 41.4. Matches aSDomain:
                     nrpspksdomains_IE135_RS12270_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short_C2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1001: short-chain dehydrogenase/reductase SDR"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452957.1"
                     /locus_tag="IE135_RS12270"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24890"
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="WP_199888147.1"
                     /sec_met_domain="adh_short (E-value: 2.8e-37, bitscore:
                     122.8, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short_C2 (E-value: 6.5e-32, bitscore:
                     105.8, seeds: 47, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPGGPLLPGKVALITGASSGIGAAAGRHFAAEGAAVVLVARRAA
                     QVEEVAERIRKDGGRAVAVPGDVTRPADMERAVATAVDRFGSLDCAFNNAGYASAGTVL
                     HELPDDVFDRTLDVNLRGVWNCLRAEIPAMLEAGAGAVVNTSSVAGVRATAASAPYVAA
                     KHAVIGLTRAAAAEYGEHGIRVNALVVGGTDTEMMDSFRSTGLTVDAGFGSQGIQRRLA
                     DPVEIARAAAWLCSDAASFTTGTALAVDGGRTAK"
     aSDomain        11890..12399
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE135_RS12270_PKS_KR.1"
                     /evalue="4.60E-13"
                     /label="IE135_RS12270_PKS_KR.1"
                     /locus_tag="IE135_RS12270"
                     /protein_end="181"
                     /protein_start="11"
                     /score="41.4"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C2"
                     /tool="antismash"
                     /translation="VALITGASSGIGAAAGRHFAAEGAAVVLVARRAAQVEEVAERIRK
                     DGGRAVAVPGDVTRPADMERAVATAVDRFGSLDCAFNNAGYASAGTVLHELPDDVFDRT
                     LDVNLRGVWNCLRAEIPAMLEAGAGAVVNTSSVAGVRATAASAPYVAAKHAVIGLTRAA
                     AAEYGEH"
     CDS_motif       11896..11985
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE135_RS12270_0001"
                     /evalue="1.60E-04"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE135_RS12270"
                     /protein_end="43"
                     /protein_start="13"
                     /score="14.3"
                     /tool="antismash"
                     /translation="LITGASSGIGAAAGRHFAAEGAAVVLVARR"
     gene            complement(12761..13177)
                     /locus_tag="IE135_RS12275"
                     /old_locus_tag="GCM10010253_24900"
     CDS             complement(12761..13177)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763175.1"
                     /locus_tag="IE135_RS12275"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24900"
                     /product="multidrug efflux SMR transporter"
                     /protein_id="WP_069735581.1"
                     /transl_table=11
                     /translation="MSNEAKTSTNAWLSLLLAGVFEIGYALAVGGSEGFTVLTWSLVAV
                     VFFLLTLYALSLALRTIEVSIGYAVWAGIGAVGAAVLGPVFFDETLTLAKGIWLSVIIV
                     GVIWLKLADRTKPETAAPAPAAQQVADRPGALQV"
     gene            complement(13259..13579)
                     /locus_tag="IE135_RS12280"
                     /old_locus_tag="GCM10010253_24910"
     CDS             complement(13259..13579)
                     /GO_function="GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003967532.1"
                     /locus_tag="IE135_RS12280"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24910"
                     /product="multidrug efflux SMR transporter"
                     /protein_id="WP_030722115.1"
                     /transl_table=11
                     /translation="MHWLYLAIAVAFEIAVAIAAGKAEGFKNRKWTAITLISGALATFF
                     LSKSLLTFDVGVGYALWTSVAGVGITILGALFFGQRLNWNKAIGIVLVIGGVVGLQLSG
                     SA"
     gene            complement(13662..14141)
                     /locus_tag="IE135_RS12285"
                     /old_locus_tag="GCM10010253_24920"
     CDS             complement(13662..14141)
                     /EC_number="3.5.4.33"
                     /GO_function="GO:0008270 - zinc ion binding [Evidence IEA]"
                     /GO_function="GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1240: riboflavin biosynthesis protein RibD"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019763177.1"
                     /locus_tag="IE135_RS12285"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_24920"
                     /product="nucleoside deaminase"
                     /protein_id="WP_069735582.1"
                     /transl_table=11
                     /translation="MGTHDSELMGFAHEAIRLSREHVAQGGIPFSGVVVGGGRILGTGF
                     NRVREDNDPTAHAEVVALRQATARHGMRAVAGATLIASGEPCALCYMAARFAGIGHIVH
                     TADRRTAARYGFDYTGSYSLFAEDPEHWPIKVTALQLPEAEVPFQDFLTMSRPRH"
     misc_feature    complement(14178..14191)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 17.22"
                     /tool="antismash"
     misc_feature    complement(14245..14258)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.36"
                     /tool="antismash"
     misc_feature    14255..14268
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 15.49"
                     /tool="antismash"
     misc_feature    14462..14477
                     /note="TFBS match to ClgR, Caseinolytic protease gene
                     regulator, confidence: weak, score: 22.92"
                     /tool="antismash"
ORIGIN
        1 gctacgcggt catcccctcg ctgggggcgt acgcgtcggc ccgcggcctc gcccggctcc
       61 agggcgtcct ggccgccgac gccgtacggg ctccgggcga ggagccgctg ctgctgtcgc
      121 ggcgcacgct cgaccaggcg cggagcttct gggccgaggg cctggacccg ctgctcggcg
      181 gggtcagcgc ctacggcgaa ggcggcctcc agctcctcga cgacgggctg cacccggccg
      241 tccgctccgg cgccttcggc caccagggcc ccgggtcggt cacctaccag gcctggcccc
      301 gggcccggac cggcgccgcc tgggtccgcg gccggctgac ggcgggcccg gccgcccggc
      361 tgctggacga ggtggccgac gcggtggacc ggtcgctgcg ggcgggctga ggaatccgcc
      421 ggcccgccgg cgggagcggt gcgagacggg ccccggccgc cgtcgggccg gggcccgtca
      481 gctctccgcc cgcgaaggct cccggtcgtc gcggacggac ccctcgcccc ggtccgcggc
      541 gaggccgtgc aggacgacgt cccagacgga gtcccagacc gcgccccccg ccgcgctccg
      601 gggcgtgccg ggggcggcca tcaccccctc cgccaggtgc gccagcatgg cctccgcctc
      661 ctcgggcgcg acgcccgggc ggagccgccg ggcaccgtgg gcccggtcga gcacggcacg
      721 gaggacgggc atccaggtct cggccaacgg cggagcgctg tccggcggac cggcttccag
      781 gcggcgtacg gcgcggacga agcggtcctc gcgcgccagc cgggccagcg tgccgatcag
      841 gacccgcagc tcccgtaacg ggtcggcggc ggggacgagt cccgccaggg agcggtcgaa
      901 gcgcgcacgt ccggtgagcc gcacctcctc ggccagggcg gtcttgttcc tgaagtggaa
      961 ggtcagagcg ccgatcgagg tctcggcccg acaggccacc cgctggagtg aggcgccttc
     1021 gtaaccccgc tcggcgaact cctcggccgc cgcctccagc acggcctgtc gggtcctggc
     1081 cgcccgttcc tgcatgcgcc ttctccttga acctcggtaa gtcgccactc ttctccctca
     1141 gaaatataac gaacattctc tatgtttgtt agtctgctca gcgacgctca cctgggaggg
     1201 acttgcagtg acgacgatgc acgaaacgcc cgagatggcg acgggccgag cgctggacga
     1261 cctgtcccgc ctcctcttcg gccacttgca ccgctccgac cagcagcgtt gggggcatgt
     1321 gtacttacag ggcctgctgc gtacgcacgg caagaagacc gtgcggcgca tggccgccac
     1381 cgtgaccggc tccgaggcga cgtcgtcgtc gctgcaccag ttcgtgaacg ccagcccctg
     1441 ggagtgggcc ccggtccgcg gccggctggc ccgctgggcc acccggtcgc tgccggtgcg
     1501 gagctggacc ctggcgccga cggtgctgcc caagcggggg aaccactcgc ccggcgtgca
     1561 ccagcgcttc ctgccggagt cggggcgcac cgtcaactgc cagctggggc tgggcctctt
     1621 cctgaacacc gggcaggcac acctcccggt cgactggcag ctcttcctgc cgccgcgctt
     1681 cacccaggac cccgccgtac gggaccgggc gaagatcccg gcgacggccc ggccgcagac
     1741 accgggggac cacgccctgc agctggtcca ggagatgacg tcctggacgg acggggagac
     1801 cgcgcccgtg gtggccgacc tgacagccct gtgcgacacc ccgcagctgg cgcggcgact
     1861 ccagggcagc gggcactcct tcgtgctcgc cgtcccgggc cagaccgcca tcggcgcagc
     1921 cgaactgccg ccgggtgagc ggccgtcggc gcgggcactg ctgctgcgcc gcggggtgcg
     1981 gcacccggcg ggggcgggcc gccggatcat ctcgtacgag acccccgtgc ccgggctccg
     2041 cccgagcggc gagagcaccc acgggcccgg ccggctgctg gccgagtggg acgcggacgc
     2101 ggggcgcccg ggccggatct ggctgaccaa catcaccgac cgccatccgg ccgaactgct
     2161 ggagctggcc cactcgcccc ggggcgcgga gtccgcggtc cggcgcatgg gcgaggactt
     2221 cggcctgctc gacttcgagg gccgctcctt ccccggctgg caccggcaca tgacactcgt
     2281 ctcggccgcc tacacgtgtg ccactctcga aggcatgaac ccctgactct tcattgcgca
     2341 tacaagtaaa tatcggccag cacccgctcc cgcaggcacc agccctcccc aaatgattgc
     2401 atgggcatgt atcttgttgg tatcgcagtc gagaagggga gctgttccat gacgatcaac
     2461 cagcagccac gcgccgcctc cgatcagacc atgtgggagg ccgttctcgg tctgcacgcc
     2521 ttcgtggaac gtcagctcgc acacaccctc cagcgccgct acggggtcgg cctgtccgag
     2581 taccgggccc tggagaccct ccaccaggcc gagaagggcg agtgccgcat gcaggaactg
     2641 gcggaccaca tcggcctggg ccagagttcg gtcacacgcc tggtgggacg gctggacagc
     2701 gccggctacg cctacaagga cagctgcgcc gacgacaagc gcggcgtctt cgccgtcatc
     2761 accgaagaag gccgcaagcg ctaccaggaa gcccgcgcca cgtacgccga cgtcctcagc
     2821 tcggccctca acaccgcgag caccgacgcc cagctcgccc gggcggtgca agcgctgcgc
     2881 agcgccgcct gacacgacca cgaggacggc gggaggcggc cggggcaacc cggccgcctc
     2941 ccgccgtcct ttgcatgttc gggcttcctt cgaaggcgac aggggtgtgg cctggatcac
     3001 acgaacgctc aatctatttg cttgaccatg caaatagaac cgcgtaggtt ctctctcgta
     3061 gccagatctc gcggcctcgc agccggatct cgcagtcgga ccgcgcggct cactcgcacg
     3121 gccggatccc gccgtgagat cgcccatcca ccgaggggga ccgttgccat cgcctcgcct
     3181 gttacaggcc ttcagcagac atgtgcagga acagcccgac agcaccgcac tcgtcgccgc
     3241 cggcgagcgg gtcagctacg cggagctgga gcggctgacc cgccgcgcca ccgccgacct
     3301 ggacggcgcc ggcttcggat ccgtccgcgc cctgtgcgtc ccggcccaca agtcaccgga
     3361 gaccatcgcc ctgctgctgg cggcctggcg tcaggggatc aacaccctgg tcccctcccc
     3421 cggtctgggc gccgccgccc tggccacgct caccgcccag gcccacggcc cgctgacggc
     3481 cacggcggtc cccggcggcg gtgtgagcct ggtgcgggag gagacggacc ccgcggcggc
     3541 ggacggcttc agcgtccccg accccgccga gtccctgctg accctcacca cctccgggtc
     3601 caccggcgta cccaagctcg tcccgatccg cacccatgcc ttcgaccggt tcgccgactg
     3661 ggccaccggg aagttcggga tcgacggcac gacccgggcg ctgagcttct cgcctctcaa
     3721 cttcgacctg gcgctcctcg acgtctggac ggtcctggag gccggcggca cggtcgtgct
     3781 ggccgacgcc ggggccagcg gggacgccgg ctatctgcgg gaactgaccc tcgcccacga
     3841 ggtgaccctc gtccaggggg tgcccctcct ccaccggctg ctggccgcgg aaggggctcg
     3901 ctacccggcg gtccgtacgg tcgtcttcac cggggaggcc gtctccgagc aggggctgcg
     3961 ggacgcgttc gcctcctgcc cgggcgcccg cttccacaac gtcttcggct gcaccgagac
     4021 caacgacagc ttcatcgagg acatcgaccc cgacgcgtac gtccatccgc tgcccatcgg
     4081 caccccggtc gacggcaccg acgcggtcct gctgatcgac gggcccgagg gcagcgaggt
     4141 cctccagggg ccgggcaccg gcgaactgct ggtgcacacc cccttccaga cctgcggcta
     4201 cctcgaacag gcccgtaacg cccgcgcctt catgcccgac ccgcgcggcg gcggcgccat
     4261 gttctaccgc accggcgacc tcgtccaccg cgacgccgac ggccgctact tcctccaggg
     4321 acgcaccgac ttccaggtca aggtgcgcgg cgtgcgcacc aacaccatgg aggtcgagac
     4381 cgtcctcggc gcccacccgg acgtcgtcga ggcctgtgtc gtcgccctgc cctgccccga
     4441 ggccggcaac cggctgcacg ccgaagtggt gcgccgggac ggggcctccc tcagctcgct
     4501 gggactgcgg gcgtacgcgg cgaagcacct gccgcggcac gccgtgccca gctcggtcgg
     4561 gctcgccggc acaccgctgc cgaagacccc gaccggcaag cccgaccgta actcgatcaa
     4621 gaagaaccga ctgaacggag acacctcatg agcaccaccg ccagcgaccc gatccgcgcc
     4681 tacatcgtca gcgagttcct cgccggcgag gacgccgagg acctgaccga cgacttcgac
     4741 ctcatcgaga gcaccgtcgt caacagcctc ggcctggtcc gcctcatctc ctggatatcc
     4801 gagacctacc gcatccccgt cgacgacatc cccctggagc cggccgccta ccgcacgctc
     4861 gcggacatcc gccgcttcgt cgagcaggcc gccccggcct ccgtctgacc ccacagctcc
     4921 acccctgcac cgcccgcccc ccccacacgc tccagcgcac cagcacacgt ccagaacaca
     4981 acgatgacag gagtacttcc atgatcgtcc gttctctctc cgacgtcccc gcggtcgagt
     5041 ggggcagcgg caccagccac cgcctgctca ccgaggccga cggtatgggc ttcaccgtct
     5101 gcgagacgct ggtccgcaag ggcacctcca gcgggcttca gtacctcggt cacctggagg
     5161 cctgctactg cataggcggc tcgggcgagg tcgtcaccac cgaaggcgtc cgccacaaga
     5221 tcaagaccgg caccatctac gccctggacc agcacgacgc ccaccacctg ctcgcctccg
     5281 acgacgagga cctgctcctc gtctccgtct tcagcccggc gctgcggggc gacgagaagc
     5341 actcgctctc cgaggacggc ttctcccgct actgatcacc gccgccgggn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnagac acctacggcg tcacagcgcc gtacggcgac
     5461 gagctgaacg acgacgtcgc ccgctgggac cgggccggcg agttcccccg cgagaagtgg
     5521 aagccgctcc agcgttcggg cctcttcacc ctccccttcg ccccggagta cggcggcgcc
     5581 ggccggacgc tcaccgagac catgtccgcg ctggagggcc tcggccacac cagccgggac
     5641 gccggcctca acttctcggc gtccacccag atcgtcagcg tcggcatccc gctccaggcg
     5701 ttcggctccg aggagctgcg ctcgcggtac ctgccgcggg tcacctccgg cgaggccatc
     5761 acggcgcacg cgatcaccga gccgagccac ggctccgacg tcatgaacat ccggaccacc
     5821 gcggtccgcg agggcgacga gtacgtgatc gacggcggca agatgttcat caccaacgcc
     5881 cccgtcgccg acctcttcct gctgtacgtc cgtaccggca agccgggcgc cttcggactg
     5941 agcaccttcc tcgccgagcg ctccacgccc ggcctgaaga tcggcgagcc gctggagacg
     6001 atggggctgc gcacctcacc catcagcgag gtcacgctgg agaacgtccg gctgcccgcc
     6061 gccaaccggc tgggcagcga gggagccggc ttcctgatca tggactacgt gatgaagcgg
     6121 gaggtcctct tctccttcgc cgtcaacctc ggagagatga cccaccgtct caagcgcgtc
     6181 gtcgaacacg ccaccacgcg ccagcagttc ggcagcgcca tcggcaagaa ccaggcggtc
     6241 tcccacaaga tcgcggacat gaggatcgcg gtggagacgg cccggaagtg gctgtacgac
     6301 accgggcgca aggtcgagca gggcacggac gcctcgctgg acgtggccgc caccaagatc
     6361 gtggtcagcg aggccaacca gctcaccgcc cagcacgcca tccagatctt cggcgcccgc
     6421 ggctatctga ccgacaccgg catcgagcgc gagctgcgca acgccacctc cggctccatc
     6481 tactccggca ccaacgagat ccagcgcaac tgcatcgccg ccctgatggg cctgtgacga
     6541 gaggtatccg ccatgaccga caccctgacc gacaccgtct ccgcaccccc ggcccccgag
     6601 gacctggacc gcctcaccgc ggccaccgac ttcctcgacc acgagcacgg gaccgtgcgc
     6661 gccttcgtcg acaaggccct ggacgggatc gaccgcgaga aggccggcca ggtcgaactc
     6721 gccgtcgccc tctactacgc ggtgcgcgac ggcatccact acgaggtcta cggcgccgac
     6781 ctctcgcccg aggggctgaa ggcgtccagc atcatcaccg gcggcaaggg cttctgcctg
     6841 cacaagtccg tgctctacgc cgccgcctgc cgctccgtcg gcatcccggc ccggctgcac
     6901 tacagcgacg tccgcaacca cctcgcctcc gaccggctgc gctcctacat cggcggcgac
     6961 gtcttcttcc acggcctggt caccgtgtat ctcgaaggcc gctggcttca ggtgaccccg
     7021 gtcttcaaca agctcctgtg ccggctgtac ggcatgaccc cgctggagtt cgacggacgc
     7081 tcggacagcc tctaccaccc cttcgacgcc caggggcggc agagcatgga gttcctcacc
     7141 gatcacggcg acttcgacga cgtgccgtac gagttcgtcc tggacaacat gcgccgcaag
     7201 cacccgaagt tcctcgacgc ggacggcacc ggcacggtca gcggcggttc gctggccgac
     7261 gaggcgaact gagccctccc ctcccccctc ccccgcagag aagaacggca gaagaagagg
     7321 agaagcaccc catgacgcag ttccaggccc gagaggacgg ctggggcgcc gtcgcccgcg
     7381 tcggtgtcgt cgttccgcac gcggacgtcg ggccggagtc cgagctccag gccatggccc
     7441 cgcgcgatgt ctacatccac ggctcccgcg tccacttcgg ggcgatgcgg cccggcggcg
     7501 agatggaccc caagatcccg cacgacccgg tgcgcgcctt catcgacccg cccttcatcg
     7561 acgacgcggt cgaactgctc gccgcctccc cgctggactc catcgccctg gggttcacca
     7621 gctccgccta cgtcctgggc gccgacggcg agcagaagct cttcgagcgg ctcgccgagc
     7681 ggacccgggg catcccgctc accggcacca cccacgcggc cacggccggc ttccgggccc
     7741 tgggggccga gcggatcgcg ctggtcaacc cgccgtggtt cgacgacgag ctgtccgccc
     7801 tgggtgcctc ctacttcggc gagcgcggct tcgacgtcgt ccaccacgcc ccctgcggtc
     7861 tccccagcaa ccagaagctg atcaccccgg aggccctggt gcagtggatc gagtccaccg
     7921 tcgccccgca gaagccgcag gcggtcctgg tcgccggcaa cggcatccgc gccgtgggca
     7981 ccatcgcggg cctggaggag cggatcggct tccccgtcct gaccgccaac caggtgctct
     8041 tctggcacgc gctgcacctc gccggcggta ccgaggcggc cgcgcggatc agcgactacg
     8101 gcgccctgtt cggcgtccgc ccggaacccg ggagcgtgac cggatgagcc tgctgcccgc
     8161 gtacggctac caggccgccg tgcaggagac ctgcgacacc cccgagtacc acatgcccct
     8221 ggtgtgctgg cacgacgacg acaacggcgt gcgcggcctg gtgctgtacc gcggggcact
     8281 gcgcccggcg gagcaggtgc cggggttccg gcgctatctc gccgccgagg gtctggagca
     8341 gtacgccacg accggcccgt ggccgctgct cgcagcaacc gcctgatccg acccgtggcg
     8401 ggggccggcc gggtccccgc cacatcagga gccgtatcga catgacacac gacctcaagt
     8461 ggtccgacgc gcccccgggg gaggtccgcg accaggtggg cgtctggatc gtccgggccc
     8521 cggccgggca gcggctgccg cccgacgggt caccgcccgg ctggctcgac gagaaggaac
     8581 ggagccggct ggccgcgata ccgcagcgct ccggcaagga ggcctacgcc ttcgcccact
     8641 gggcgctgcg cagcgtcctc ggctcctcac tcggctgccc gccccggggc gtcgccttcc
     8701 tgcggcagcc gtgtcccggc tgcggcgggg cgcacggcag gccggccatc cgtgatccgg
     8761 accggggcgg cgaacccccg gagttctcga tgtcgcacag cggggaccac gtcgccatcg
     8821 cggtggccga ggccaccatc ggcatcgaca tcgagtgcca tccgaccccg gagtcggtgg
     8881 cgggctatct gccctttctc cacccgaggg aacagcagtg gctgggcggc cgtccggccg
     8941 atgaactggt ccgcgacttc gcccggttgt ggacccgtaa ggaggccatg gccaaggcga
     9001 ccggccaggg catggcggcg gtcatgaacc ggctcgacct caccggacag ccgctcggct
     9061 ggcgggtgag gcagctgatc gccccacccg ggtacgaggc cagcttcgcc gttccggcgt
     9121 cggtgcacgt ggacgtcatc atgtacgagg acctcccgca cccgatgccg gaggcggagc
     9181 tggagccggt ctgaccggtg gcgggcggtc cgtacctccg agcgaggagg tacggaccgc
     9241 ccgccgcgtc acgtcgtggg ccccggggag caggccctca tccggcgatg gtcacctcgg
     9301 aggtgaagac ctggacgccg tcctgctcga tcgagacggg cacccggcgc ctgccggggc
     9361 cgacgggtgc cggggaaccg gccacgacca cggccggagc gtcgagttcg acgtaccgga
     9421 cgaagttcgt ccgcattccg atgaccgctg cggtggcccc gccgacggcg cgcgccgcct
     9481 gccgggcggc ctccagcagg agcatcccgg gcgcgtggtc gaccgggtgg tcgaagagca
     9541 ccgggtgggc ggtgtccacc cgcagcagcc accggccggg tctctcgccc cccagggccg
     9601 gcgagaggac cacgtcggcc ggggaaccgc ggcccacccg gtgcggctcc accggcgggc
     9661 ccgccgggag gcggttctcc agtgcggcgt cgatgtccgc gtagcccctg cgcattctcc
     9721 ggtagacggc cgcgggctgg tcgttgaagc gggtgtgcgc gatccccatg agtcgcttgc
     9781 cgaggtggac gtcgatgtcc atcgtcatgc ccgcgaacct gttgccgcgc cggaccacgt
     9841 ccgagcagga caccaccaga cgcacctcgt cctcggcggt ggtgaccagg gcggccagcg
     9901 gctcgaccgc gaagtctaag cactcccacg cctgcttgtg ctcccgagga acgccgtgca
     9961 cggcgtggga gagcagcggc actgtctggc gcacggtctc cgcgagcagc agcggatcgt
    10021 acgcgccctg cgacggctgg tagaacgcgt gccccttcgg ccagcgggcc gtgatcacga
    10081 agcgtccgga gtcttcctcg gtccacgtcc tgagcagtac ttcctccccc cggagcttgt
    10141 gcacctgcca ctggtgcacc tgagactgct ggaccggctg tacgtcacgg ctcagtacgg
    10201 aaacagacaa cgtggacccc ccacgatcgg tcgttgctca cggatgactg cgcagaggag
    10261 cgacatgagc accccccgtg ccgactcaaa aataatgttt cttttgtttt gctgggtcaa
    10321 gagcaacgcg agcctgactt tttttggcgt ccaggagtat gcataagcaa cttaactgga
    10381 acaaacggga cttgccctgg cgccggtccg gccggataac ataagggtca ttctttttgg
    10441 gaaggtgaca gggcatggca caaccgaaac aggagcgagc ggagcagacc cgccgcgctc
    10501 tcctccacgc ggcagcagag gtgttcgacg agttcggcta cgcgggtgca agcatcacgc
    10561 gcatcctcaa gcgcgccggt gtgacggccg gagcgctcta cttccacttc ggctccaagg
    10621 aggacctcgc caaggcggtg atgaacagcc agccggagac cctggtcccg ctggtggccg
    10681 ccgggggcct gcaacggctc atcgacatca cgctcgtctg gtcgtggcag ctccagcgcg
    10741 atccactgct gcgggccggc gtccggctga ccaacgagca aacctccgtg ggcggtccgg
    10801 ccaacgccga cccgtacgag aagttccgcg cgatcatgac cgggtgcctc caggacgccc
    10861 agaacgacgg cgagctccag cccggcgtcg acccggtcgt ggtcgcggag ttcgtggtcg
    10921 cggcctgtac cgggatgcag atgtactcca acgtcgtcag cggccgccag gaccttcccg
    10981 agcgcacgca gcagatgtgg aacctcatcc ttccgggcat cgcggtcccg tccgtgatca
    11041 cgcgcgccga cgcgacccct gcccggggcg ccgcgatgat gccctgaccg ctcagcccgc
    11101 cgaccgttcc tcccgcctcg aacgagcctc aaggagcccc atgctttcct tacgcctggc
    11161 cgccctgaac atcgacggtg tcctgctcaa cgacaccttc agcccggtca tccaccagtt
    11221 cgtcgtcagc cggggcggga cctactcggc cgcgctggag cgatcggtct tctcccagtc
    11281 gcagcacata gccgggcaac ggctcgcagc cgccgtgggc ggcggcatga cgggcgaggc
    11341 ggcgctcgcc gcctacttcg aggagcgcgc cgcccacgtc ggccggcacc cggtcacgat
    11401 caccccgggc acgcaccgcc tgctcctccg gctgcggagc gcgggcctgc acacggtctg
    11461 ctacggaggc ctgggcaagg agcacttcga ccgccatctg ggccgctacg cccacctgtt
    11521 cgacggcccc ggctacgtca gcaccaacga catccgcccc ggtctccggg agatcaccga
    11581 ggacgtcttc gggctccgtc ccgcccaggc cctcttcgtc gacgacgtcg cccgggtcgc
    11641 cgaggaggcc cgcgcgttgg gcaccgcctt cgtcggtcac ccctccgcct tcgcccacag
    11701 ccaccagcgc accctcatgc gggaggcggg cgtacgccac atggtcgacc gcctggacgc
    11761 gctcgacgac ggtctgctgc accggatcga ccaggagatg agcacgggca cgttctggag
    11821 ccccgccctc gtggcggccg gccgggagag acaggcgtga cgccgggcgg accgctgctg
    11881 cccggcaagg tggcgctgat caccggcgcc tccagcggca taggcgccgc ggccggccgc
    11941 cacttcgccg ccgagggagc cgccgtcgtg ctcgtcgccc gccgggccgc ccaggtggag
    12001 gaggtcgccg agcgcatccg gaaggacggc ggccgggccg tggccgtacc gggtgacgtc
    12061 acccgccccg cggacatgga acgggccgtc gccacggcgg tcgaccggtt cggcagcctc
    12121 gactgcgcgt tcaacaacgc cggatacgcc agcgccggaa cggtcctgca cgaactcccc
    12181 gacgacgtct tcgaccgcac cctggacgtc aatctgcgcg gtgtgtggaa ctgcctgcgc
    12241 gccgagatcc ccgccatgct ggaggccggc gccggagcgg tcgtcaacac gtccagcgtc
    12301 gcgggcgtac gggccaccgc cgcgtcggcg ccctatgtgg ccgcgaagca cgcggtcatc
    12361 ggcctcacca gggcggcggc cgccgagtac ggcgagcacg gcatccgggt caacgccctc
    12421 gtggtgggcg gcaccgacac ggagatgatg gactccttcc gctccaccgg cctcaccgtg
    12481 gacgcgggtt tcggctcgca gggcatccag cggcggctgg ccgatccggt cgagatcgcc
    12541 cgcgccgccg cctggctctg ctcggacgcc gcctccttca ccaccgggac cgccctcgcg
    12601 gtggacggtg gacggacggc gaaatgacga cggagcccac gcccgggacg gcccggaccg
    12661 cctcgtgagg acagcggcgc gcggtacgac ggcacgcccc cggcggctgc cgcccattcc
    12721 tgatgggagg cagccgccgg gggcgtacgt tcgcggggcg tcagacctgg agcgcgccgg
    12781 gccggtccgc gacctgctgg gccgccgggg ccggggcggc cgtctcgggc ttcgtacggt
    12841 cggcgagctt cagccagatg acaccgacga tgatgaccga cagccagatg cccttggcca
    12901 gggtcagggt ctcgtcgaag aagacggggc ccaggacggc ggcgccgacc gcgccgatgc
    12961 ccgcccagac ggcgtatccg atgctgacct cgatcgtgcg gagggcgagg ctcagtgcgt
    13021 agagcgtcag caggaagaac accacggcga ccagggacca ggtcaggacg gtgaaccctt
    13081 cgctgccgcc gacggccagc gcgtagccga tctcgaagac tccggcgagc aggagcgaga
    13141 gccaggcgtt ggtggaggtc ttggcttcgt tggacatggg aggtggatcc tctctggggt
    13201 ggcgtcagat ggtgggcggg aggcaccgcg tgtgcggtcg ggggtggtgc acccgggctc
    13261 aggcggaacc gctcagctgg aggccgacga ctccaccgat gacgaggacg atgccgatcg
    13321 ccttgttcca gttcaggcgc tggccgaaga agagcgcgcc gaggatggtg atgccgaccc
    13381 ccgccacgga ggtccagagg gcgtagccga cgccgacgtc gaaggtcagc agcgacttgc
    13441 tgaggaagaa ggtcgccagg gcaccgctga tcagcgtgat ggcggtccac ttgcggttct
    13501 tgaagccctc cgcctttccg gcggcgatgg cgacagcgat ctcgaaggcg acggcgatgg
    13561 cgaggtagag ccagtgcatg ggaggtcctt ccgggcggtg ggtgaggagg agttcgggcg
    13621 tgggggacgg cgtgcggcgg tgtcgcggaa ccgggtccgg gtcagtgccg gggccggctc
    13681 atggtgagga agtcctggaa cggcacttcc gcttcgggga gctggagcgc cgtgaccttg
    13741 atgggccagt gctcggggtc ctcggcgaac agggagtacg agccggtgta gtcgaagccg
    13801 taccgggcgg cggtccggcg gtcggcggtg tgcacgatgt ggccgatgcc ggcgaaccgc
    13861 gccgccatgt agcacagggc gcagggctcg ccggaggcga tcagggtggc tcccgccacg
    13921 gcgcgcatgc cgtgccgcgc ggtggcctgc cggagggcga cgacctcggc gtgcgcggtc
    13981 gggtcgttgt cctcgcggac ccggttgaag ccggtaccca ggatgcgtcc gccgcccacg
    14041 acgacaccgg agaagggaat gccgccctgg gcgacgtgct cgcggctgag ccggatcgct
    14101 tcgtgggcga agcccatcag ttcgctgtcg tgtgtaccca tgagcagttc gtctcccgag
    14161 ttgccctgta tctattttgc ctgcacatgc aagcatatag tttgcgtgga tctccgccag
    14221 cggcgtgacc cgatgcctta atcttgcatg tgcatataac gagtgtcaag cggacgcata
    14281 cgtaagggcg ttgtccaacg tcgccagagg ctccagcacc tgcgggtcga acgccgtcgg
    14341 cagagccccc gcggccgcct tgtgcggcag gtcggggttg gccagcgcgc ccgtaccgat
    14401 cgccaggacg tccgcgtgcc cctcgtccag gacgcgccgt gccaccgctt cctcgtggag
    14461 cccgccgttg gcgatcacgg gcactccggt gaccttccgg gcgagcccgg gaagcgtggg
    14521 gccgtccggc agccagtccc gtccgctgcc ggcgaggtgc agatagtcgg ccccggcccc
    14581 gaccgccgcc ccgaagagtg cggccgcctc ggcgtcgccc ccctgccacc gccagccgaa
    14641 gttgttgatc ttcccctggg agagccggac gccgaccgtg aagccgggtc cggccgcggc
    14701 tctgaccgcg gccgtcacct cccgcaggag ccgtgagcgc ccctcgatct ccccgccgta
    14761 cgcgtcggtg cgcagattgg tgtacggggt gaggaactgg tcgagcaggt agccgttggc
    14821 ggcgtggatc tcgacgccgt cgaagccggc gccacgggcg cgtaccgctg cggtggcgaa
    14881 cccttcgaca gcctcgccga tctggtcgga ggtcatctcc agcggtacgg gccacgggcc
    14941 gctgccccgg tacttggcca gctgctcacc catcggacgg accgccgacg ggccggcggt
    15001 ccggtcccgg aaccggttcc cctgggccag ggccccggcg tgcatcagct ggaggacgat
    15061 gcgcgcgccg gcggcgtgcg cgcggtcgac gacgccccgc caggcggcgg cctgtgcctc
    15121 gtccgtgatg ccgggctggt gcaggtagcc ctggccgaag gcggtgtcgg tgtaggtccc
    15181 ctcggtgatc aggagcccga aaccaccctc
//
