LOCUS       NZ_BMSZ01000005        11347 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces badius strain JCM 4350 sequence05, whole genome shotgun
            sequence.
ACCESSION   NZ_BMSZ01000005
VERSION     NZ_BMSZ01000005.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces badius
  ORGANISM  Streptomyces badius
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 368305)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 154X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649415.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:57:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,894
            CDSs (total)                      :: 6,825
            Genes (coding)                    :: 6,582
            CDSs (with protein)               :: 6,582
            Genes (RNA)                       :: 69
            rRNAs                             :: 2 (23S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 70 of 244
            Pseudo Genes (incomplete)         :: 206 of 244
            Pseudo Genes (internal stop)      :: 7 of 244
            Pseudo Genes (multiple problems)  :: 38 of 244
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:11:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 296275
            Orig. end                         :: 307622
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSZ01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..11347
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="RiPP-like"
                     /region_number="3"
                     /rules="(strepbact or Antimicrobial14 or Bacteriocin_IId or
                     BacteriocIIc_cy or Bacteriocin_II or Bacteriocin_IIi or
                     Lactococcin or Antimicrobial17 or Lactococcin_972 or
                     Bacteriocin_IIc or LcnG-beta or Cloacin or Linocin_M18 or
                     TIGR03651 or TIGR03693 or TIGR03601 or TIGR03795 or
                     TIGR03975 or DUF692 or TIGR01193 or (YcaO or TIGR03604))"
                     /tool="antismash"
     cand_cluster    1..11347
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /kind="single"
                     /product="RiPP-like"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..11347
                     /aStool="rule-based-clusters"
                     /category="RiPP"
                     /contig_edge="False"
                     /core_location="[5000:6347](-)"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(5001..6347)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(strepbact or Antimicrobial14 or
                     Bacteriocin_IId or BacteriocIIc_cy or Bacteriocin_II or
                     Bacteriocin_IIi or Lactococcin or Antimicrobial17 or
                     Lactococcin_972 or Bacteriocin_IIc or LcnG-beta or Cloacin
                     or Linocin_M18 or TIGR03651 or TIGR03693 or TIGR03601 or
                     TIGR03795 or TIGR03975 or DUF692 or TIGR01193 or (YcaO or
                     TIGR03604))"
                     /neighbourhood="5000"
                     /product="RiPP-like"
                     /protocluster_number="1"
     misc_feature    complement(936..952)
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 15.3"
                     /tool="antismash"
     gene            1363..2091
                     /gene="hemQ"
                     /locus_tag="IE135_RS11340"
                     /old_locus_tag="GCM10010253_22990"
     CDS             1363..2091
                     /EC_number="1.3.98.5"
                     /codon_start=1
                     /gene="hemQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965515.1"
                     /locus_tag="IE135_RS11340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_22990"
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="WP_006128414.1"
                     /transl_table=11
                     /translation="MSAPETVTSSKSPNAGKKAKDLNEVIRYTLWSVFKLRDVLPLDRA
                     GYADEVQELFDQLAAKDITVRGTYDLSGLRADADIMIWWHAETSDQLQDAYNLFRRTKL
                     GRALDPVWSNMALHRPAEFNKSHIPAFLADETPRDYISVYPFVRSYDWYLLPDEDRRRM
                     LADHGKMARGYPDVRANTVASFSLGDYEWILAFEADELYRIVDLMRHLRASEARLHVRE
                     EVPFYTGRRKSVADLVAGLA"
     gene            complement(2252..3814)
                     /locus_tag="IE135_RS11345"
                     /old_locus_tag="GCM10010253_23000"
     CDS             complement(2252..3814)
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /NRPS_PKS="Domain: Abhydrolase_1 (101-476). E-value:
                     1.6e-19. Score: 62.4. Matches aSDomain:
                     nrpspksdomains_IE135_RS11345_Abhydrolase_1.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF00561"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018956907.1"
                     /locus_tag="IE135_RS11345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_23000"
                     /product="alpha/beta hydrolase"
                     /protein_id="WP_128818879.1"
                     /sec_met_domain="PF00561 (E-value: 4.5e-26, bitscore: 86.3,
                     seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRTAALYTSIGSLVLSALAAAPAGAWDRPGSAEARGTAIAAARAT
                     DAGIDFAACPEEEMLPDSLKCGTVKVPLDYADPDGRQLELTVSRTPAAGPAKERQGAFV
                     YNPGGPGASSITFPLAGELPEWKEIAEAYDLVGYAPRGVNGSSAPLSCQDPAAYTKGPT
                     DAPTHPTQEDKERRVARAKAYARGCAEHAGETLRHYTSLNNARDLDVLRAALGESRLTF
                     MGASYGTYFGALYATLFPSHVRRMVFDSAVDPDPEQIWYRSNMGQSLAFEARWEDFRRW
                     VAKHDDVYHLGTTPEAVQRRYDEVRAELAVKPAGGKVGPGQLHSAFLGAGYYDDYWAMR
                     ATALSEYVRGNPEPLVAQASPRAVAAKDNENAQAVYTAVECNDAPWPEDFEVWDRDHTE
                     LARVAPFETWDNAFSNLPCAFWPAPRQRPLDVRVGWGELPPVLILAAERDAATPYRGAL
                     ELRKRLPGSSLVTERDAGTHGIGGAGNACVDDHLRRYLLTGEVPGRGADCAAHPEPNPV
                     SLD"
     aSDomain        complement(2387..3511)
                     /aSDomain="Abhydrolase_1"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE135_RS11345_Abhydrolase_1.1"
                     /evalue="1.60E-19"
                     /label="IE135_RS11345_Abhydrolase_1.1"
                     /locus_tag="IE135_RS11345"
                     /protein_end="476"
                     /protein_start="101"
                     /score="62.4"
                     /tool="antismash"
                     /translation="AFVYNPGGPGASSITFPLAGELPEWKEIAEAYDLVGYAPRGVNGS
                     SAPLSCQDPAAYTKGPTDAPTHPTQEDKERRVARAKAYARGCAEHAGETLRHYTSLNNA
                     RDLDVLRAALGESRLTFMGASYGTYFGALYATLFPSHVRRMVFDSAVDPDPEQIWYRSN
                     MGQSLAFEARWEDFRRWVAKHDDVYHLGTTPEAVQRRYDEVRAELAVKPAGGKVGPGQL
                     HSAFLGAGYYDDYWAMRATALSEYVRGNPEPLVAQASPRAVAAKDNENAQAVYTAVECN
                     DAPWPEDFEVWDRDHTELARVAPFETWDNAFSNLPCAFWPAPRQRPLDVRVGWGELPPV
                     LILAAERDAATPYRGALELRKRLPGSSLVTERDAG"
     gene            complement(3973..4995)
                     /locus_tag="IE135_RS11350"
                     /old_locus_tag="GCM10010253_23010"
     CDS             complement(3973..4995)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965513.1"
                     /locus_tag="IE135_RS11350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_23010"
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="WP_199888056.1"
                     /transl_table=11
                     /translation="MNVLALLATLGVVVSSVLLVVTTVAERFRRPSAGPGVALHDLYEV
                     AFLNGGPARVVDTALTALHADGRLAVGGPGIVAVQREQANDPVERAVFEALADAPNGAL
                     HALREAVMRHPAVQEVGDGLAARGLLVPPHRLRPLRRWGLTQGVACLIALPVSVVLTFV
                     QFAATDPLEISVPFIVKMLPAIVIGAFTGLIVTVSSASRLTKAGRHAALSFRTSYAHVA
                     TPAHLVATLGLRALPDPVFQGQLVAAARLSAPRRRSSSYVAGGTAAGATAVAATTVWCA
                     ASGPGGPSCGGSTGCGSGSSCGGGSGCGGGGGSSCGGGSGCGGSSGSSCGGGSSCGGGS
                     "
     gene            complement(5001..6347)
                     /locus_tag="IE135_RS11355"
                     /old_locus_tag="GCM10010253_23020"
     CDS             complement(5001..6347)
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     RiPP-like: DUF692"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072529.1"
                     /locus_tag="IE135_RS11355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_23020"
                     /product="DUF692 domain-containing protein"
                     /protein_id="WP_199888057.1"
                     /sec_met_domain="DUF692 (E-value: 2e-94, bitscore: 310.0,
                     seeds: 7, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELGIGIGWRPEIAAEVEALSGIDWVEAVAENLCADHLPDSLVRL
                     RERGVTVVPHGVALGLGGADRPDPDRLAGLAARAELLGAPLVSEHIAFVRAGGPLTASP
                     RLEAGHLLPVPRTRDALDVLCENVRIAQDSLPVPLALENIAALITWPDEELTEGQFLAE
                     LVERTGVRLLIDVANLHTNHVNLGQDPAKALDELPVEAIAYVHVAGGVEKNGVWHDTHA
                     HPVSEPVLEVLAELRSRVDPPGVLLERDDAFPPGAELAGELDAIRATLRKAAPSAGQGP
                     DRAAGRGAAPAADATAGVETPTVAAAPAPAPVGIRDRTAVAQTALLSALVAGTPAPEGF
                     DHRRLRVQSRALAAKRADVVAKVAPELPEILGDGYRAAFLAYAGSRPLSGGYRRDALDF
                     AEHVLITGGPADPVARRRLTSWWQDRSGARPPRRTTRLVRAARAVLVGK"
     gene            complement(6469..7215)
                     /locus_tag="IE135_RS11360"
                     /old_locus_tag="GCM10010253_23030"
     CDS             complement(6469..7215)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072530.1"
                     /locus_tag="IE135_RS11360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_23030"
                     /product="DUF4142 domain-containing protein"
                     /protein_id="WP_176734541.1"
                     /transl_table=11
                     /translation="MRRINGTALIIAALVATLGAIAFPVWSYADRSGTGEANLNASSVA
                     TQWGPLSATDRDFLVKVRLAGLWELPAGQQAIERAPSEATKAAGDHLVVGHTDLDRRAR
                     DVAAKLGVELPNQPTAQQQEWLRELTAASGDAYERKFANILRAAHGKVFGLIGQVRHTT
                     RNTLIRQLASDANQTVLDHITMLEATGFVDFDALAREAASGSTASPSGPSMPRDGQAPL
                     APVPATPTGDQSFTSRPVPPTMMPMP"
     gene            complement(7356..8084)
                     /locus_tag="IE135_RS11365"
                     /old_locus_tag="GCM10010253_23040"
     CDS             complement(7356..8084)
                     /GO_function="GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072531.1"
                     /locus_tag="IE135_RS11365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_23040"
                     /product="peptidyl-tRNA hydrolase"
                     /protein_id="WP_069739273.1"
                     /transl_table=11
                     /translation="MTSHDTPSPASGSPFRSGPTDRDQAPQFVLPLVLHLEKTDPPART
                     DALRAAARAVLTILSDERSLGDGEWAAAMRDWQDARIRKVVRRARGAEWRKAAALDGIT
                     VTGEDGAEVRVFPPIPLDGWPKELAKLQVSGTDLDDPEPPAAPDPSGPVLWLNPDVDMS
                     AGKTMAQAGHGAQLAWWELSDTERKAWREAGFPLSVATPGAERWRELTASGLPVVRDAG
                     FTEIAPGSCTVIADHPALRR"
     gene            8156..8830
                     /locus_tag="IE135_RS11370"
                     /old_locus_tag="GCM10010253_23050"
     CDS             8156..8830
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378552.1"
                     /locus_tag="IE135_RS11370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_23050"
                     /product="AIM24 family protein"
                     /protein_id="WP_069739274.1"
                     /transl_table=11
                     /translation="MKSDLFSSEHMAQPATAPGMTLQNAKSIKYAVNGEMHARQGSMIA
                     FRGNLQFERKGQGIGGMLKRAVTGEGLALMAVRGQGEAWFAHEAANCFIVELDQGDAIT
                     INGRNVLCFDPTLSYEIKTVKGAGMVGGGLFNSVFTGYGKLGLMCEGTPIVIPVTAAQP
                     VYVDTDAVVGWSQQLTTSLHRSQSVGSMVRGGSGEAVQLMLQGEGFVIVRPSEARPEKT
                     SN"
     gene            8833..9750
                     /locus_tag="IE135_RS11375"
                     /old_locus_tag="GCM10010253_23060"
     CDS             8833..9750
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088893.1"
                     /locus_tag="IE135_RS11375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_23060"
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="WP_199888058.1"
                     /transl_table=11
                     /translation="MELRGVGHRHNPGGRWVLRGIDLALPARALVRVEGGNGMGKSTLL
                     RLLAGIEAPAEGRITGRPARTAYVPERFPAALPLTVTGYLVHLGRIHGLRTAEAKRRAG
                     EWLTRFGAAEHARTPLAELSKGTSQKVAVAQALLAAPDLLVLDEAWTGLDTAARTELDR
                     AVAERVAAGAAVVFVDHDPRRLAGAADVTLRMGGGGVHRATSSPATVPVTGPRTLIEAE
                     GPPGAPLPRDLPGDPLRERGAGTAPGLVRLTVPTPYSDALLTALLTARPPWHIRRVAPV
                     DAPERPGPPEPVRPSEQPEVSEAP"
     misc_feature    8965..8967
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            9747..10415
                     /locus_tag="IE135_RS11380"
                     /old_locus_tag="GCM10010253_23070"
     CDS             9747..10415
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007452202.1"
                     /locus_tag="IE135_RS11380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_23070"
                     /product="ABC transporter"
                     /protein_id="WP_069739936.1"
                     /transl_table=11
                     /translation="MTALLRYQAALLARSQRWLAPLLLYVAVLAVGVQNGQPVLGSLGF
                     AAAALLPVTAWAVHLCVGQEPPAARAIASAAAGPRRVHLAALLTATGGAAVLAGVTTAV
                     VLLVSSPATDNGTQAVPRPAAALAGLLAALCCVLLGAAVGALSTRPVLRRRGWSVAAAV
                     LGSLLALVTTGSPARYAVTALVTGSRTGTFDPPVLASLGAAASAGTAAFVVCRLAARRG
                     "
     gene            10424..10621
                     /locus_tag="IE135_RS11385"
                     /old_locus_tag="GCM10010253_23080"
     CDS             10424..10621
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006128422.1"
                     /locus_tag="IE135_RS11385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_23080"
                     /product="hypothetical protein"
                     /protein_id="WP_107474863.1"
                     /transl_table=11
                     /translation="MDVDDTYCEPPGRIEPAPPAGPAEGPSYAECVLCREPTEHPESTK
                     GATLCPVCTWQEAGRTACSG"
     gene            complement(10680..10991)
                     /locus_tag="IE135_RS11390"
                     /old_locus_tag="GCM10010253_23090"
     CDS             complement(10680..10991)
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE135_RS11390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010253_23090"
                     /product="hypothetical protein"
                     /protein_id="WP_069739935.1"
                     /transl_table=11
                     /translation="MQFDENEPQAMREERFAAERAARRARRKAAKLDSRDAHLGAAMLV
                     VALTLLGWFVQWVALAVVSGVFLLWFAAALSWTYADGDHGRHALQRAYNLTFGWGGGF"
ORIGIN
        1 tgcgggtcac cctcctggag gccacggacc ggctcggcgg caagctcatg accggcgagg
       61 tcgcgggcgt ccaggtcgac ctgggggccg agtcgatgct cgcccggcgc cccgaggcgg
      121 tcgcgctcgc cgaagcggtg ggcctcggcg accggctcca gccgcccgcc accgccaccg
      181 cgtcgctctg gacgcgcgac gcgctgcgcc ccatgcccaa gggccacgtc atgggcgtcc
      241 ccggcgaccc ggcggtcctc ggcgaggtgc tgtcccccga gggcctggcc cggatcgccg
      301 aggaccgcga cctcaccccg gcccccgtcg gcgacgacgt cgcggtcggc gcgtacgtcg
      361 ccgaccgtct cggccgcgaa gtcgtggacc ggctcgtgga gccccttctc ggcggggtct
      421 acgcgggcga cgcctaccgc atctcgatgc gcgccgccgt accccagctc ttcgaggccg
      481 tgaaggaggg cggcccgctg ctcgacggcg tccgccgcat ccaggaacgg gccgccgccc
      541 ggcagcagac cggcccggtc ttccagggca tcgcgggcgg catcggcacc ctcccggccg
      601 ccgtcggcga cgccgtacgg gccgggggcg gcgacatcct caccgagacg cccgtgctcg
      661 gcctgacccg tacggaaacg ggttgggccg tccgcaccga cacccggacg atcgccgccg
      721 acggcatcgt cctggccacc cccgcctggt ccgcgggcgc cctgctggcc gcggagtccc
      781 cgaacgcctc cgccgagctg tccggcgtcg agtacgcctc gatggccctg gtcaccctgg
      841 ccttccggcg gaccgacatc gaggcgtacg aagccctgcg gggccgctcc ggcttcctcg
      901 tgccgccggt cgacgggcac acgatcaagg cgtccacgtt ctccagcaac aagtggcagt
      961 gggtcgccga cgcagccccc gacctgttcg tgctccgtac gtccgtcggc cgctacggcg
     1021 aggaggacca cctccaccgc gaggaccagg agctggtctc cgtctccctg cgcgacctcg
     1081 ccgccgccac cggcctcacc gcccggcccg tcgacaccga ggtcacccgc tgggtcggcg
     1141 gcctgccgca gtacccggtc ggccatctgg cccgggtcgc ccgcatccgc gacgaggtcg
     1201 ccaaactgcc cgccctgcgc gtctgcggcg cggtctacga cggggtgggc atccccgcct
     1261 gcatcgcgag cgcccgccgc gcggccgacg agatcgcgag cgagatcatc gccacgccga
     1321 ccctggttcg gggcacgggg agcgaggcgg gacaatagcc gtatgagtgc tcctgagact
     1381 gtgacatcaa gcaagagtcc gaacgccggc aagaaggcca aggacctcaa cgaggtcatc
     1441 cgctacaccc tgtggtcggt cttcaagctc cgcgacgtcc tcccgctgga ccgcgcgggc
     1501 tacgccgacg aggtgcagga gctgttcgac cagctcgcgg ccaaggacat caccgtccgg
     1561 ggcacctacg acctgtccgg gctgcgggcc gacgccgaca tcatgatctg gtggcacgcc
     1621 gagacctcgg accagctcca ggacgcgtac aacctcttca ggcgcaccaa gctcggccgc
     1681 gcgctcgacc cggtctggtc gaacatggcg ctgcaccgtc ccgccgagtt caacaagtcg
     1741 cacatcccgg cgttcctcgc cgacgagacg ccccgcgact acatcagcgt ctaccccttc
     1801 gtccggtcct acgactggta cctgctgccc gacgaggacc gccgccgcat gctcgcggac
     1861 cacggcaaga tggcccgggg ctaccccgac gtccgcgcca acaccgtcgc ctcgttctcc
     1921 ctcggcgact acgagtggat cctcgccttc gaggcggacg agctgtaccg catcgtcgac
     1981 ctgatgcgtc acctgcgcgc ctccgaggca cggctccacg tgcgcgagga ggtcccgttc
     2041 tacacgggcc gcaggaagag cgtcgcagac ctggtggccg ggctcgcata gccagcgagt
     2101 cccgcaccac cccaacccgg aagatcagag agtcggacac cgggcaccga tcgtgccgtc
     2161 cgcctctcca gcgacaccgg gttcggcttc gggggccgtc gcaccgtgaa ggtgcggcgg
     2221 cccccgctcc gtacgcccgc cgaccgccgg atcagtccag cgacaccggg ttgggctccg
     2281 ggtgggcggc gcagtccgcc ccccgccccg gcacctcacc ggtcagcaga taccggcgca
     2341 gatgatcgtc gacgcaggcg ttccccgccc cgccgatgcc atgcgtcccc gcgtcccgct
     2401 cggtcaccag tgacgaaccg ggcagccgct tccgcagttc cagcgccccc cggtacgggg
     2461 tcgccgcgtc ccgctccgcc gccaggatca gcaccggcgg cagctcgccc caccccacgc
     2521 ggacgtccag cggccgctgc cggggagccg gccagaaggc gcacggcagg ttcgagaagg
     2581 cgttgtccca ggtctcgaag ggcgcgacgc gcgcgagctc cgtgtggtcg cggtcccaga
     2641 cctcgaagtc ctcgggccag ggcgcgtcat tgcactcgac ggccgtgtac accgcctggg
     2701 cgttctcgtt gtccttcgcc gccaccgcgc gcggggacgc ctgcgcgacc agcggctccg
     2761 gattgccccg gacgtactcc gacagcgccg tcgcccgcat cgcccagtag tcgtcgtagt
     2821 agcccgcccc caggaacgcc gaatgcagct gccccggccc gaccttgccg cccgcaggct
     2881 tcaccgccag ctcggcgcgc acctcgtcgt agcgccgctg caccgcctcg ggggtggtcc
     2941 ccaggtggta cacgtcgtcg tgcttggcga cccagcggcg gaagtcctcc cagcgcgcct
     3001 cgaaggccag cgactgaccc atgttggagc ggtaccagat ctgctccggg tcgggatcga
     3061 ccgccgagtc gaagaccatg cgccgtacgt gggaggggaa cagcgtcgcg tacagcgccc
     3121 cgaagtacgt cccgtacgac gcccccatga acgtcagccg gctctcgccg agcgcggccc
     3181 gcagcacgtc caggtcccgg gcgttgttca gcgaggtgta gtgccgcagc gtctcgcccg
     3241 cgtgctccgc gcagccgcgc gcatacgcct tcgcacgggc gacgcgccgc tccttgtcct
     3301 cctgggtcgg atgcgtcggc gcgtcggtcg gacccttcgt gtacgcggca gggtcctggc
     3361 aactcaacgg ggcggaggag ccgttcaccc cacgcggagc gtagccgacc aggtcgtacg
     3421 cctcggcgat ctccttccac tccggcagct ccccggccag cgggaaggtg atgctggacg
     3481 cgccggggcc gcccgggttg tagacgaacg ccccctgccg ctccttcgcc ggaccggcgg
     3541 cgggcgtccg gctgacggtc agttcgagct gccgtccgtc cggatccgcg tagtcgaggg
     3601 gcaccttgac cgtgccgcac ttcagggagt ccgggagcat ctcctcctcc gggcaggcgg
     3661 cgaagtcgat cccggcgtcc gtggcccggg cggcggcgat cgcggtgccc cgggcctcgg
     3721 cggaaccggg gcggtcccag gcccccgcag gggcggcggc gagggcggac aggaccaggg
     3781 agccgatcga tgtgtagagc gcagcagttc tcacgcgcgg gggcctctct gcggatgcgg
     3841 agaggaatgg tcgggcggcc ggagggacat gtccagtacc gccccggagg gtcaccggga
     3901 accactccga tgggcggtca agatgcttgc gtacgggccc gattcaggtt cgggagaggg
     3961 aggggggccg gttcaggacc cgcccccaca gctggagcca ccaccgcagc tggaaccgct
     4021 cgaaccaccg cagccggaac cgccaccaca gctcgacccg cctccgcctc cgcacccgga
     4081 cccgccgccg cagctcgaac cggagccgca cccggtcgac ccgccgcagc tggggccgcc
     4141 ggggccggag gcggcgcacc agaccgtcgt ggcggcgacc gcggtggccc ccgccgccgt
     4201 gccgccggcg acgtacgacg aggaccgccg acgcggcgcg ctcagccgtg ccgctgccac
     4261 caactggccc tggaagaccg ggtccggcag cgcgcgcagt cccagcgtgg cgacgagatg
     4321 tgcgggggtc gccacatggg cgtaggaggt ccggaacgac agcgcggcgt gccgccccgc
     4381 cttcgtgagg cggctcgcgg aggagaccgt gacgatcagc ccggtgaacg cgccgatgac
     4441 gatcgcgggc agcatcttca cgatgaacgg gaccgagatt tccagggggt cggtcgcggc
     4501 gaactggacg aacgtgagca ccacgctcac cggcagggcg atgaggcacg ccaccccctg
     4561 ggtcaacccc caccgccgca gcggacggag ccggtgagga ggcaccagca gtccacgcgc
     4621 ggcgagcccg tcgccgacct cctggaccgc cgggtgccgc atcaccgctt cccgcagggc
     4681 gtgcagggcg ccgttcgggg cgtcggccag cgcctcgaac accgcgcgct ccaccgggtc
     4741 gttcgcctgc tcccgctgta cggcgacgat gccgggcccg ccgacggcca gccggccgtc
     4801 cgcgtgcagg gcggtcaggg cggtgtccac gaccctggcc ggacccccgt tcaggaacgc
     4861 gacctcgtac aggtcgtgaa gagcgacgcc ggggccggca gaggggcggc ggaaccgctc
     4921 ggccaccgtc gtgacgacga gcagcaccga tgagacgacg accccgaggg tcgccagcag
     4981 agccaagacg ttcacagcgg tcacttcccc acgaggacgg cacgggccgc ccggaccaga
     5041 cgggtggtac ggcgcggcgg ccgggcgccc gaccggtcct gccaccagga cgtcagccgg
     5101 cgccgcgcca ccggatcggc gggaccgccc gtgatgagca catgctccgc gaagtccagg
     5161 gcgtcgcggc gatagccgcc ggacaggggg cggctgccgg cgtacgcgag gaacgcggcg
     5221 cggtagccgt cgccgaggat ctccggcagt tcgggtgcca ccttggcgac gacgtcggcg
     5281 cgcttggccg ccagtgcccg gctctgcacc cgcagccgcc ggtggtcgaa gccctcgggg
     5341 gccggggttc cggcgacgag ggcggagagc agggcggtct gtgcgacggc ggtacggtca
     5401 cggatgccga cgggtgcggg ggcaggtgcg gccgcgaccg taggggtttc gacgcccgcg
     5461 gtggcgtcgg ccgcgggagc ggcaccccgg cccgccgccc ggtccggccc ctggcccgcc
     5521 gagggtgccg ccttccgcag cgtcgcccgg atcgcgtcca gttcgcccgc cagctccgcg
     5581 cccggcggga aggcgtcgtc ccgctccagc agtacgccgg gcggatcgac ccgggagcgc
     5641 agctcggcca gcacctccag gacgggttcg gacaccgggt gggcgtgcgt gtcgtgccag
     5701 acgccgttct tctcgacccc gcccgccaca tggacgtacg cgatcgcctc caccggcagt
     5761 tcgtccaggg ccttcgccgg atcctggccg agattgacgt ggttggtgtg cagattggcc
     5821 acgtcgatca gcagccgcac cccggtccgc tcgaccagct ccgccaggaa ctgcccctcg
     5881 gtgagctcct cgtccggcca ggtgatcagc gcggcgatgt tctccagggc gagcggcacc
     5941 ggcagtgagt cctgcgcgat ccgcacgttc tcgcagagca cgtccagcgc gtcccgggtg
     6001 cgcggcaccg gcagcaggtg ccccgcctcc agcctcggcg acgcggtcag cggccccccg
     6061 gcccgtacga acgcgatgtg ctcgctcacc aggggcgccc ccagcagttc ggcgcgcgcc
     6121 gcgagaccgg cgagccggtc ggggtcgggc cggtccgcgc cgccgaggcc gagtgccacc
     6181 ccgtgcggca cgacggtcac cccgcgctcc cggagccgta cgagcgagtc cgggaggtgg
     6241 tcggcgcaga ggttctccgc gaccgcctcc acccagtcga tcccgctcag cgcctccacc
     6301 tcggccgcga tctccggccg ccagccgatg ccgatgccca gttccatggt gcccccgtcc
     6361 ccctgctcac ctcgtgctgt tcaggggtca tggccccgtc gcgcggcgat gaacccgagc
     6421 ggtgggacgt tcagagctac atttgaggtt ccgggcccga cgccgtcgtt atggcatcgg
     6481 catcatcgtg ggcggcaccg ggcgcgaggt gaaggactgg tcgccggtcg gcgtcgccgg
     6541 aaccggagcc agcggggcct ggccgtcccg gggcatcgac gggccggaag gactggccgt
     6601 cgacccgctc gccgcttcgc gcgccagggc gtcgaagtcg acgaagccgg tcgcctccag
     6661 catggtgatg tggtccagca cggtctggtt ggcgtcgctc gccagctgcc ggatcagcgt
     6721 gttccgggtg gtgtggcgga cctggccgat caggccgaag accttgccgt gtgcggcgcg
     6781 caggatgttg gcgaacttcc gctcgtacgc gtccccgctc gcggccgtca gctcccgcag
     6841 ccattcctgc tgctgggcgg tcggctggtt gggcagctcc accccgagct tcgccgcgac
     6901 gtcccgggcc cgccggtcca ggtccgtgtg cccgacgacg aggtggtccc cggcggcctt
     6961 cgtcgcctcg ctgggcgccc gttcgatcgc ctgctgcccg gcgggcagct cccacagccc
     7021 ggcgagccgc accttgacca ggaagtcccg gtcggtcgcg gagagcggcc cccactgcgt
     7081 ggcgacactg gaggcgttga gattcgcctc gccggtgccc gaacggtcgg cgtaggacca
     7141 gacagggaac gcgatggcgc cgagtgtggc gaccagtgcc gcgatgatga gggccgtgcc
     7201 gttgatgcgt cgcaacaagg tgtctcccgg gccgaggcgt gacagcgtcg atcgaacagg
     7261 cgtcaatcaa ccgccaatcg actggctgtg caggctactt ggcggtagtg ccgatagggc
     7321 agtaccggga gatacgcagg aggaggcaga agtgttcagc ggcgcagcgc cgggtggtcg
     7381 gcgatcacgg tgcaggagcc cggcgcgatc tcggtgaacc ccgcgtcgcg caccaccggc
     7441 agcccgctcg ccgtcagctc ccgccagcgc tccgccccgg gggtggccac ggagagcggg
     7501 aacccggcct cgcgccaggc cttgcgctcg gtgtcggaca gctcccacca ggcgagctga
     7561 gccccgtgcc cggcctgcgc catcgtcttg cccgccgaca tgtcgacatc ggggttcagc
     7621 cacagcaccg ggcccgaggg atcgggcgcg gcggggggct cggggtcgtc caggtcggtg
     7681 cccgacacct ggagcttggc cagctccttg ggccagccgt ccagcgggat cggcgggaac
     7741 acccgtacct ccgcgccgtc ctcgcccgtc accgtgatgc cgtcgagcgc cgccgccttg
     7801 cgccactccg cgccgcgcgc ccggcgcacg accttgcgga tccgggcgtc ctgccagtcc
     7861 cgcatggccg ccgcccactc gccgtcgccg agggaccgct cgtcggagag gatcgtcagc
     7921 accgcccggg cggcggcccg cagcgcgtcg gtgcgggcgg gcgggtccgt cttctccagg
     7981 tggagcacca gaggcagcac gaactgcggt gcctgatcac ggtcggtcgg gccgctgcgg
     8041 aacgggctcc cggaggcggg ggagggggtg tcatggctgg tcaccgtccc agtctgccag
     8101 gcggacgagg tcatccgctt ggcggaacgg ccgggtccgg gcgaggatgc tccgcatgaa
     8161 gagcgatctc ttttccagcg agcacatggc ccagccggcc accgcccccg ggatgaccct
     8221 gcagaacgcc aagtcgatca agtacgccgt caacggggag atgcacgccc ggcagggctc
     8281 gatgatcgcc ttccgcggca atctccagtt cgagcggaag ggccagggca tcggcggcat
     8341 gctcaagcgc gccgtcaccg gcgagggcct cgcgctgatg gccgtccggg gccaggggga
     8401 ggcctggttc gcgcacgagg cggcgaactg tttcatcgtg gagctggacc agggcgacgc
     8461 catcaccatc aacggccgca acgtcctgtg cttcgacccc acgctctcct acgagatcaa
     8521 gaccgtgaag ggcgccggga tggtcggcgg cggcctcttc aacagcgtct tcacgggcta
     8581 cggcaagctc ggcctgatgt gcgagggcac cccgatcgtg atccccgtga cggccgccca
     8641 gccggtgtat gtggacacgg acgcggtcgt cggctggagc cagcagctca cgaccagcct
     8701 gcaccgctca cagagcgtcg gctcgatggt ccgcggcggc tcgggcgagg cggtccagct
     8761 gatgctccag ggcgagggtt tcgtcatcgt ccggcccagc gaggcccggc ccgagaagac
     8821 gtccaactga ccttggagct gcggggcgtg ggccaccgcc acaaccccgg cggccgctgg
     8881 gtgctgcgcg gaatcgacct cgccctgccc gcacgggccc tcgtccgcgt cgagggcggc
     8941 aacggcatgg gcaagtcgac cttgttacgg ctgctcgccg ggatcgaggc ccccgccgag
     9001 gggcggatca ccggccgccc ggcccgcacg gcgtacgtcc ccgaacgctt cccggccgcg
     9061 ctgcccctca ccgtcaccgg ctacctcgtc cacctcggcc ggatccacgg actgcggacc
     9121 gccgaggcga agcggcgggc gggggagtgg ctgacccggt tcggggcggc ggagcacgcc
     9181 cgtaccccgc tcgccgaact ctccaagggc accagccaga aggtcgccgt cgcccaggcg
     9241 ctgctcgccg caccggacct gctggtcctg gacgaggcat ggaccgggct cgacaccgcc
     9301 gcccgcaccg aactcgaccg tgccgtggcc gaacgcgtcg cggccggcgc cgccgtcgtc
     9361 ttcgtcgacc acgacccgcg acgcctcgcc ggggcggccg acgtcaccct gcggatgggg
     9421 ggcggcgggg tccaccgtgc gacgtcatca ccggcgaccg tccccgtcac cggcccccgc
     9481 accctgatcg aggccgaggg cccgcccggc gcgccgctgc cccgggacct cccgggcgac
     9541 ccgctccgcg aacgaggggc gggaacggcc cccggcctcg tacgcctcac cgtcccgacg
     9601 ccgtactccg acgccctgct gacggccttg ctgacggccc gtccgccgtg gcacatccgg
     9661 cgggtggcgc ctgtcgacgc acccgaacgg cctggtccgc ctgagccggt acggccgtcc
     9721 gaacagcccg aggtgtccga agcgccatga ccgccctcct ccgctaccag gccgccctgc
     9781 tcgcccgctc ccagcgctgg ctggcgcccc tcctgctgta cgtggccgtc ctcgccgtcg
     9841 gcgtgcagaa cgggcagccc gtgctgggct cgctcggttt cgcggccgcc gccctgctgc
     9901 ccgtcaccgc gtgggccgtc cacctctgcg tcggccagga gccgcccgcc gcccgggcga
     9961 tcgcctcggc cgccgccgga ccccgacggg tccacctggc cgcgctgctc accgcgaccg
    10021 gcggtgcggc ggtgctcgcc ggagtgacca ccgcggtggt cctgctggtc agcagcccgg
    10081 ccaccgacaa cggcacccag gccgtccccc gcccggccgc cgcgctcgcc ggactgctcg
    10141 cggccctgtg ctgcgtgctg ctgggcgcgg ccgtgggggc gctcagcacc cgcccggtgc
    10201 tgcgccgccg gggctggtcg gtcgcggcgg ccgtgctcgg ctcgctcctc gcgctcgtga
    10261 ccaccggctc cccggcgcgg tacgcggtca ccgccctggt caccggatcc cggaccggga
    10321 cgttcgaccc gcccgtcctg gcgtcgctgg gggcggcggc gtccgccggg acagcggcgt
    10381 tcgtcgtctg ccggctcgcc gcccggcgtg gctgagtagg ctcgtggacg tggacgacac
    10441 ctactgcgag cccccggggc ggatcgagcc cgccccgccc gccggacccg ccgaaggccc
    10501 ttcgtacgcc gaatgcgtgc tgtgccgcga gccgaccgag cacccggagt cgacgaaggg
    10561 cgccacgctc tgcccggtct gcacctggca agaggcgggc cggacggcgt gttccggcta
    10621 gggcgtactc ggcggggcct ttgtgctcgt ggccgcgccg ggccgcgcac gaggggtgcc
    10681 tagaacccgc cgccccaccc gaaggtgagg ttgtacgcgc gctggagcgc gtgccggccg
    10741 tggtcgccgt ctgcgtacgt ccaggagagg gccgcggcga accagagcag gaacaccccg
    10801 ctcacgacgg ccagagcgac ccactggacg aaccagccga ggagtgtcag ggccacgacc
    10861 agcatggccg cgccgaggtg cgcatcccgc gagtcgagtt tcgcggcctt gcgtctcgct
    10921 ctgcgcgccg ctcgctccgc ggcgaaacgt tcctcgcgca tggcctgtgg ttcgttctcg
    10981 tcgaactgca ccgtgccccc cctcgtccca ggcactgaag atacttgcgt tcggaagggg
    11041 cacgtcgggc agtcctcacg tcagtcgcgg tcctccatca gctccgacac cttcacgaac
    11101 cggtagccgc gctcgcgcag ctccggcacg atccggcgta ccgccgcctc ggtggccggg
    11161 gccgcgctgc gggtgcagtg catcaccacc agcgagcccg gccggacccc gtccagcacc
    11221 tgctccgcga ccgcgtccgc gtccgtggcg aaggcgtcgc cgctgaccac gtcccactgg
    11281 acggccgtca cgttcgccgg ggccagggcg cgcagggccg tgtcgtcgta acagccgccg
    11341 gggaagc
//
