LOCUS       NZ_BMSZ01000005        12931 bp    DNA     linear   CON 02-SEP-2024
DEFINITION  Streptomyces badius strain JCM 4350 sequence05, whole genome shotgun
            sequence.
ACCESSION   NZ_BMSZ01000005
VERSION     NZ_BMSZ01000005.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces badius
  ORGANISM  Streptomyces badius
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 368305)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: BGI assembly pipeline
            Genome Coverage                   :: 154X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649415.1-RS_2024_09_02
            Annotation Date                   :: 09/02/2024 03:57:07
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.8
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,894
            CDSs (total)                      :: 6,825
            Genes (coding)                    :: 6,582
            CDSs (with protein)               :: 6,582
            Genes (RNA)                       :: 69
            rRNAs                             :: 2 (23S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 64
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 244
            CDSs (without protein)            :: 243
            Pseudo Genes (ambiguous residues) :: 0 of 244
            Pseudo Genes (frameshifted)       :: 70 of 244
            Pseudo Genes (incomplete)         :: 206 of 244
            Pseudo Genes (internal stop)      :: 7 of 244
            Pseudo Genes (multiple problems)  :: 38 of 244
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-13 16:11:55
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 97648
            Orig. end                         :: 110579
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSZ01000005.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..12931
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="hydrogen-cyanide"
                     /region_number="2"
                     /rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD) and DAO)"
                     /tool="antismash"
     cand_cluster    1..12931
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /kind="single"
                     /product="hydrogen-cyanide"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..12931
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:7931](+)"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..7931
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="5000"
                     /detection_rule="(Fer2_4 and cds(Pyr_redox_2 and Fer2_BFD)
                     and DAO)"
                     /neighbourhood="5000"
                     /product="hydrogen-cyanide"
                     /protocluster_number="1"
     misc_feature    39..55
                     /note="TFBS match to NtrC, Nitrogen regulatory protein C,
                     confidence: medium, score: 24.47"
                     /tool="antismash"
     misc_feature    complement(855..868)
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.87"
                     /tool="antismash"
     misc_feature    complement(1990..2005)
                     /note="TFBS match to LexA_variant_1, Repressor of DNA
                     damage response, confidence: weak, score: 20.36"
                     /tool="antismash"
     gene            complement(2080..3258)
                     /locus_tag="IE135_RS10540"
                     /old_locus_tag="GCM10010253_21320"
     CDS             complement(2080..3258)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072430.1"
                     /locus_tag="IE135_RS10540"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_21320"
                     /product="CapA family protein"
                     /protein_id="WP_069736869.1"
                     /transl_table=11
                     /translation="MGRAAGLLAIAVGLTVSCGSQPAQPEAKAGGRSFTVAAAGDVLIH
                     PELVEQAAKDAEKSGRGEAGLDFGPLLAGVKPVISKADLAICHMETPVGNPKGPFQGYP
                     EFLVPPQILTSLKDVGYDTCSTASNHTFDHGLKAVRRTLDAMDKVGLGHSGSARTPKEA
                     EQINIRDVNGVKVAHLSYSWESFLNPTPEKQSWAFNRSRTEDIKKDEKRARDKGAEVVL
                     LSLHWGLEHYNEPSVPQLDMAQRITEETGIDLVIGHHAHVVQPIQKVNGTWVAYSLGNQ
                     VARHSSPTGLTEEGAIGWFEFRETADGWDVSARYRTTLVDVPPEIEPGEKAPEGAVEDL
                     RLVDVQQALENPEGLPADRTARYRLAEDRTRGFLYNRGAPGGDGLKRLSLEK"
     misc_feature    3349..3364
                     /note="TFBS match to ColR, Phenol-responsive regulator,
                     confidence: weak, score: 17.09"
                     /tool="antismash"
     gene            3366..4277
                     /locus_tag="IE135_RS10545"
                     /old_locus_tag="GCM10010253_21330"
     CDS             3366..4277
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072431.1"
                     /locus_tag="IE135_RS10545"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_21330"
                     /product="hypothetical protein"
                     /protein_id="WP_199887990.1"
                     /transl_table=11
                     /translation="MGLLLALVGCQTEPSPAPEDTVPRARELVDLRSRILGYTAKFRAD
                     APYRPPGKEQRERLAKAVGSLLSGDAQGAERRLVPLGLGVTRLTDTDSGRRYDEIAATG
                     PGESARWGRLYLNADSTVRWNAQVPHPVSDRDTEDLGIRLLEQNPGGALVIAGSHRRAG
                     GNGEADVAHREDSAFHTIVVELQKRGVPGVQLHGFSDSSDRPYDAVVSTGAVEAAPAEV
                     VALADRMDDEGLRVCRAWAARCPLEGRSNVQGRSAEREHAGFVHVELARHARADGGRDT
                     EEAAEALAGLVAGWNAGGSPTR"
     gene            complement(<4493..4735)
                     /locus_tag="IE135_RS35185"
                     /pseudo=""
     CDS             complement(<4493..4735)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF012398.5"
                     /locus_tag="IE135_RS35185"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /product="hypothetical protein"
                     /pseudo=""
                     /transl_table=11
                     /translation="MAAGTGGRRRAATVTTGEPFDPAYRDASPEQVAEAAGCPVEVKAH
                     PAHPGTGEAMARAALAAAALTGVPAGVFALLVGRGH"
     misc_feature    complement(4934..4954)
                     /note="TFBS match to MntR, Manganese transport regulator,
                     confidence: weak, score: 21.93"
                     /tool="antismash"
     misc_feature    complement(4956..4966)
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: strong, score: 22.4"
                     /tool="antismash"
     gene            5001..6161
                     /locus_tag="IE135_RS10555"
                     /old_locus_tag="GCM10010253_21340"
     CDS             5001..6161
                     /EC_number="1.-.-.-"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: DAO"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Trp_halogenase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1103: FAD dependent oxidoreductase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454561.1"
                     /locus_tag="IE135_RS10555"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_21340"
                     /product="FAD-binding oxidoreductase"
                     /protein_id="WP_199887991.1"
                     /sec_met_domain="DAO (E-value: 5.7e-65, bitscore: 214.4,
                     seeds: 521, tool: rule-based-clusters)"
                     /sec_met_domain="Trp_halogenase (E-value: 2.1e-06,
                     bitscore: 21.1, seeds: 23, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELVIVGAGIVGAACAFHAASAGLGVTVLDRGPVGAGTTSRGEGN
                     ILLSDKEPGPELELARLSRDLWGEASEELGARSVEFESKGGLVAASTPEGLAALHAFAA
                     RQAAAGVRTELVTCARGFEPHIAPGLPGAVHYPQDAQVQPVLAAAGLLQAAVRRGARVR
                     TGEAVAAVTGTGGRITGVRTADGSVLAADAVVNAAGTWGGEVGRRLGAPVEILPRRGFV
                     LVTEPLPPMIRHKVYSADYVANVASNGEGLETSCVVEGTRAGTILIGASRERVGFDTAM
                     NPDVVALLAAQACRLFPFLRDVHLIRAYRGFRPYCPDHLPVVGPDPRVPGVLHACGHEG
                     AGIGLAPGTGALITAQLLGRPWRGADPAAHTGLLPDRFLTTGGVPK"
     gene            6158..6448
                     /locus_tag="IE135_RS10560"
                     /old_locus_tag="GCM10010253_21350"
     CDS             6158..6448
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_4"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006128205.1"
                     /locus_tag="IE135_RS10560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_21350"
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="WP_069741330.1"
                     /sec_met_domain="Fer2_4 (E-value: 8e-24, bitscore: 77.9,
                     seeds: 114, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTGAAAETAATDVPTVTVDGEPLAFVPGQTLAAALVASGRVAWRT
                     TRGGQRPRGVFCGIGVCYDCLVAVDGRRGQRACLVRARAGMAVATEEGDDD"
     gene            6441..7931
                     /locus_tag="IE135_RS10565"
                     /old_locus_tag="GCM10010253_21360"
     CDS             6441..7931
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Fer2_BFD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     hydrogen-cyanide: Pyr_redox_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1176: alkyl hydroperoxide reductase subunit"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378675.1"
                     /locus_tag="IE135_RS10565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_21360"
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="WP_199887992.1"
                     /sec_met_domain="Pyr_redox_2 (E-value: 2.7e-20, bitscore:
                     67.0, seeds: 64, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_BFD (E-value: 3.2e-13, bitscore:
                     44.2, seeds: 211, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTEPRDLGGLPAMQKPHDLVVLGAGPAGMAAAATALDGGLRVALV
                     DAGPAPGGQFWRHPPDHARDAVPTEDLHHDLRTYRALRATLAAHERTGRLTLLLDHHVW
                     TTARAVDGFAVHAVDRGRPPEERAVVLRAPALLVATGAYDRQLPFPGWDLPGVLTAGGL
                     QSLLKGGGVVAGRRVVLGGTGPFLLPVAAALAARGAEVVAVCEAAAPSAWLRHPAPLLR
                     NPAKWAEAAGYAGTLARHRIPVRTRTGIIGAEGAGRVAVVRTASLGADGAPLPGTERRI
                     EADTVGVGWGFVPQLDLLLPLGCDLADAGDGTTAAAVDAGQRTTVPGLYAAGETCGVGG
                     AALALSEGRVAAVSVLTDLSAPGRPGIRPLAAERRSVARHRAFARALAQAHPVPRNWPA
                     WLTDDTTVCRCEEVTAGAVRAARADGPATDHRQVKQLTRAGMGWCQGRMCGPAVHCLVA
                     ARAESYTPAERLIATPVTLGALADSSTFPTGDTPTEPT"
     gene            7941..8855
                     /locus_tag="IE135_RS10570"
                     /old_locus_tag="GCM10010253_21370"
     CDS             7941..8855
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010072436.1"
                     /locus_tag="IE135_RS10570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_21370"
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="WP_199887993.1"
                     /transl_table=11
                     /translation="MNHVDRTAPARMPWHGVIVATSLPFDRDLAVDHGAYGDHVAWLAA
                     QGLHGVAPNGSLGEYQTLTYEERDRVVETAVAHAPEGFTVMPGVGAYGAIEARRHALFA
                     KDAGCQAVMCLPPNAYRADDRAVLEHYAMVASAGLPVTAYNNPIDTKVDLRPELLAKLY
                     AEGFIVAVKEFSGDVRRCYAISELAPGLDLIIGTDDTLLEVGVAGAKGWVAGYPQVFPR
                     ACLDLYDAALAGDLATALPLYRRLHSVLRWDSRTEFVQAIKLGQDMIGRTGGVCRPPRR
                     PLDPETEAVVRSATRALIDAGVN"
     gene            8857..9858
                     /locus_tag="IE135_RS10575"
                     /old_locus_tag="GCM10010253_21380"
     CDS             8857..9858
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378674.1"
                     /locus_tag="IE135_RS10575"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_21380"
                     /product="proline racemase family protein"
                     /protein_id="WP_128818809.1"
                     /transl_table=11
                     /translation="MLSTVCYHAVDSHTEGMPTRVITGGVGVLPGATMAERRQRFMAER
                     DGLRTLLMCEPRGHGAMSGAILQPPTRPDADFGVLFIEVSGCLPMCGHGTIGVATVLVE
                     TGMVEAVEPETLIRLDTPAGLVTARVAVRDGRAESVTLENVASYSHALDQVVDVAGFGP
                     VRYDMAYGGNFYAIVRTEDLGIPFDRAEKGRLLDAGLAVMRAVNEQNPVVHPENPAIDV
                     CHHVYLEAPGSTAEHSRHAMAIHPGWFDRSPCGTGTSARMAQLHARGLLKTGQDFVNES
                     FIGSRFTGRILDESTVGGRPAVLPSVTGRAWITGTAQYLLDPNDPYPEGFTV"
     gene            complement(9937..10647)
                     /locus_tag="IE135_RS10580"
                     /old_locus_tag="GCM10010253_21390"
     CDS             complement(9937..10647)
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1071: GntR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006128212.1"
                     /locus_tag="IE135_RS10580"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_21390"
                     /product="GntR family transcriptional regulator"
                     /protein_id="WP_128818810.1"
                     /transl_table=11
                     /translation="MSELKSRKLISVQEHLRDQVAGALRAALIAGELQPGAIYSAPTLA
                     AEYGVSATPVREAMLDLAREGLVEAVRNKGFRVTELSERDLDEYTEIRALIEIPTIGRV
                     TRTASREQLEAVRPVAEEIVAAAQRGDLIGYLESDRRFHLDLLALAGNARLVETVADLR
                     KRSRLYGLTDLSQEGSLVGSAREHTALLDIMIEGDAEAAEEHMRRHLAHVRTLWAARKR
                     EAERAAPSRKLGAR"
     misc_feature    10674..10690
                     /note="TFBS match to ZuR_variant_2, Zinc-responsive
                     repressor, confidence: weak, score: 16.35"
                     /tool="antismash"
     misc_feature    10678..10688
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 20.42"
                     /tool="antismash"
     gene            complement(10731..12191)
                     /locus_tag="IE135_RS10585"
                     /old_locus_tag="GCM10010253_21400"
     CDS             complement(10731..12191)
                     /EC_number="3.4.-.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_function="GO:0004177 - aminopeptidase activity
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1172: methionine aminopeptidase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965659.1"
                     /locus_tag="IE135_RS10585"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010253_21400"
                     /product="aminopeptidase P family protein"
                     /protein_id="WP_199887994.1"
                     /transl_table=11
                     /translation="MTSTPPARLHTGSHDLPVPAALDAFMATGWAPSPLPADARVPGHA
                     LLPGRRQRLSARFPGERLVIPAGTLKVRSNDTDHRFRPHSAYAWLTGLTGEEQPDHVLV
                     LEPSGPDGHEAVLHIRPRSPRDTSGFYRDRRYGEFWVGRRPDLDETAALTGLRCAHLDG
                     LPRLLAGPQPPTRLLAGVDPHIDDLLPARSHTPFGDAELDSVLAELRLLKDPWEVSQLQ
                     LAVDHTTSGFEDVARSLPDALRHPRGERWIEGVFNLRARTEGNGTGYETIAAAGAHACV
                     LHWIRNDGPLSPSQLLLLDAGVETDTLYTADITRTLPLSGRFSPAQRDVYELVLAAQEA
                     AIAVLRPGARFRDFHREAMRVIAEGLHAWGVLPVAPEEALADDSGLYRRYTLCSSGHML
                     GIDVHDCARARVETYLDGVLEEGHVLTVEPGLYLQPDDETLPPELRGMGVRIEDDLVIT
                     ADGAKLLSGALPRTADGVEEWMARLLDD"
     misc_feature    12312..12322
                     /note="TFBS match to HypR, L-hydroxyproline utilization
                     repressor, confidence: weak, score: 18.94"
                     /tool="antismash"
ORIGIN
        1 ggatgagcag cccgttctcc agcggcccgg cgcccacctg cacattggcg gcgcgcacca
       61 ggtcgggcag cgcgtcgttc ggcagcccct cgaaggcgcg gaatccgtcg ctggcggcga
      121 cggtccgcgc gtccgcgttc acgatccgga cctcgccgag gccgggccgc ttcagcagcg
      181 agttgaggaa ctccacccgc acctcgccga ccagggtcat gcccttgcgc tccggcacgg
      241 gcgccccggc ctggacgacg ggcctcttct cgccctggcc ggagacgcgg accagcgacg
      301 ggtccttctc cgcgctccac gggtgggtgt cgccgccggc ccgggcgacg atgtcgccgt
      361 tcgcgccgac gacgtacagc gcctggtagc gggtgtgctc gcgcagggcg ttcttcagca
      421 cggtctcggt gccggcgggg tcgtcgacgt cgagcaggcg ggaggtggag acgaggtcgg
      481 cctgggcctc gttcagcgcc cggcgggccc ggtcggcgac caggtcggtg cggtcgcgct
      541 ggtcgttgac catgacggcg gggatgctga cggtcccgtc cgtgcggttg agcaggaggc
      601 ctacgggaac gcaccacagc agcaggagga ccgccgtcag cgccaacggt ccgcgcgcgc
      661 cgccccggaa ccggccgggc gggcgggcgc ccacggggcc gtcggaggcc tgagcaccgc
      721 cgagctggcc gcggatacgt tccagcgcgg cgccgatccg ggcggcctcg ccccagcggg
      781 gcacggtgac cgggcggatc aggtcgccgc gggcgagccg gcgggactcc aggaacagtc
      841 cgagcagcgg gcgctgcacg gtcatccaca gcacggcgac cgcgagcagg ccgagcagca
      901 ccagggcgcc cgccgcgagc agcccgaaga gctgccggct gtcgtcggtg aaggtctgct
      961 gctggaccac cgggagcagg gagacgaccg tgaggccgag accggcggcg acgctctccc
     1021 cgtcctccgg gtcggaggcg gcgagggagg cgtaccccac ggtggcgacc cggccgttgt
     1081 agcccccgcc caccagggtt ccgctgaccc cggggtagcc gcgggaaccg ggctcccggg
     1141 cgctgaccgg gttctgctcg gtctcctcgg cggcctggtc ggacagccgg gtcatctgct
     1201 tctggaggaa ggtgagttcc ttgcgctccg cgtccgagtt cagcgcctcg gactgctcga
     1261 agccggcggt cgcgaggatc tcgccaccgc gcgcgacgac ggccatgctg cggaacgggc
     1321 ccaggttgat gggcggcacg gagaggctgt tggaggcgac cagcaactgc tgcgccccct
     1381 gcttcccctc cagcacggcc atcgtcatca gtcgtacgtc gccggtctcc agccgcacca
     1441 tccgcggggt gagcgccttg tcaccggtga ggacgtcctt gtccacccag gcgagcggga
     1501 tgcgctcccc gcgggcggcg agcaccttgc cgtccgccag gtccagcacc gtggtgccgg
     1561 tccacttctg gtacgccttg ctcaggtccg acagcacgcg ctcggggtcg gccggcttgc
     1621 ccgtgttcag ggcggccgcg gtgcgttcga ggtccgtgac ccgctcgtcg atcgaggcgc
     1681 gcagggcgat ggcgccgtcc tcggcgaagt actgctggga ggagagcacc gccttcggca
     1741 cgaccggttc cgagcccggc cccagcacct tggccgtgag ccccgcgagg gcgaggatca
     1801 gcacgcacag caacgcgatc ggcggccgga tgccacccag cagcggcatg tccgcacgtc
     1861 tgcggctgcg ccgccggcct ttcggcacga ggggcgcggc aggagtcgat gtcaccggtt
     1921 tcttccttgg tcgctgcgtg tgctcagtgc ccggaggaag caccgctgtc gcgtggctgc
     1981 gagacgctgt cgtagagaag ttcgagacgc tgtcatagga aagacggaca agcgcctaag
     2041 aggttgtact catcggcata tgagcgggtg tgtgcctggt cacttctcca gggacagccg
     2101 cttcaggccg tcaccgcccg gagctccccg gttgtagagg aagccgcgcg tgcggtcctc
     2161 ggccaggcgg tagcgcgcgg tccggtcggc gggcagccct tcggggttct ccagtgcctg
     2221 ctgtacgtcc accagccgga ggtcctcgac ggcgccctcg ggggccttct ccccgggttc
     2281 gatctccggc ggaacgtcga cgagcgtggt gcggtagcgg gccgagacgt cccagccgtc
     2341 ggcggtctcg cggaactcga accagccgat ggccccctcc tcggtgaggc cggtcggcga
     2401 ggagtgccgg gccacctggt tgccgaggct gtaggcgacc caggtgccgt tgaccttctg
     2461 gatcggctgc accacgtggg cgtggtgccc gatcaccagg tcgataccgg tctcctcggt
     2521 gatccgctgg gccatgtcca gctgcgggac gctcggctcg ttgtagtgct ccaggcccca
     2581 gtgcagggag agcagtacca cctcggcgcc cttgtcgcgg gcccgcttct cgtccttctt
     2641 gatgtcctcg gtacgggaac ggttgaaggc ccagctctgc ttctcgggcg tggggttgag
     2701 gaaggactcc caggagtacg agaggtgagc gaccttgacg ccgttgacgt cccggatgtt
     2761 gatctgctct gcttccttcg gcgtccgcgc cgacccgctg tgcccgaggc ccaccttgtc
     2821 catggcgtcc agcgtgcggc gcacggcttt cagtccatgg tcgaaggtgt gattggaggc
     2881 tgtcgagcag gtgtcgtatc cgacgtcttt caggctggtc aggatctgcg gcggaaccag
     2941 gaattccgga tatccctgga acggcccctt ggggttcccg acaggtgtct ccatgtgaca
     3001 gatcgcgagg tcggccttgc tgatcactgg cttcaccccg gcgagcagcg gcccgaaatc
     3061 caggccggcc tccccgcgtc cgctcttctc cgcatccttg gcggcctgct cgaccagctc
     3121 gggatggatg aggacgtctc cggccgcggc gacggtgaaa ctgcggccgc cggccttggc
     3181 ctcgggttgc gccggctgcg agccgcacga cacggtcagg ccgacggcta tcgccagcag
     3241 gcccgccgcc cgacccatac cgagagaatt acggaatgtc actgagtcat acttacatgc
     3301 gcctcgtgtg tgatcgtcgt attgccatca cgggacgatc acgtttcctc cgcatttttg
     3361 tcccggtggg tttgctcctg gctctggtcg gttgccagac cgaaccgtcc ccggccccgg
     3421 aagacaccgt tccgcgcgcc cgggaactgg tcgacctgcg cagccgcatt ctcggataca
     3481 ccgcgaagtt ccgcgccgac gccccctacc gcccgcccgg caaggagcag cgggagcggc
     3541 tggcgaaggc cgtcgggagt ctgctgtcgg gtgacgccca gggcgccgag cgccggctcg
     3601 tcccgctggg cctcggtgtg acccgcctca ccgacaccga ctcgggtcgc cgctacgacg
     3661 agatcgccgc caccgggccg ggcgagagcg cccgctgggg ccgcctttac ctgaacgccg
     3721 actccaccgt ccgctggaac gcccaggtcc cgcaccccgt ctccgaccgc gacaccgagg
     3781 acctcggtat ccggctcctg gagcagaacc ccggcggcgc tctcgtcatc gccggatccc
     3841 accggcgggc cggcggcaac ggcgaggccg atgtggccca ccgcgaggac agcgccttcc
     3901 acacgatcgt cgtggagttg cagaagcgtg gggtgcccgg cgtccagctg cacggcttca
     3961 gcgactcctc cgaccgcccc tacgacgccg tggtctccac cggcgccgtc gaagccgcgc
     4021 ccgccgaggt ggtcgccctg gccgaccgta tggacgacga ggggctgcgg gtctgccggg
     4081 cctgggcggc gcgctgcccg ctggagggca ggagcaatgt ccagggccgc tcggcggagc
     4141 gcgaacacgc cggcttcgtc cacgtcgaac tggcccgcca cgcccgcgcg gacggcggcc
     4201 gcgacacgga ggaggcggcc gaggccctgg ccggactggt cgcggggtgg aatgccggcg
     4261 gtagcccgac gcggtgatcg tgatcggtcc ggacggcgag cggcatcgag cagctgaacg
     4321 ccgaccgcct cggcgcgggc ggcggcggca tccattgcgc cactgagcag cggcccgtgc
     4381 cctgtggcgc ggcacggcgg cggcgaccag gggcgccggg cccgccgacc cggggcccgg
     4441 tgaagcccgc cgcggcgacc ctcggatccc cgaccggggc gaggctcgcc tcgtggcccc
     4501 ggcccaccag tagcgcgaac accccggccg gtacgccggt gagcgcggcg gcggccaggg
     4561 cggcgcgggc catcgcctcg ccggtgccgg ggtgggccgg atgcgccttg acctccacgg
     4621 ggcacccggc cgcctccgcg acctgttccg gcgaggcgtc ccggtacgcg gggtcgaacg
     4681 gttcgccggt cgtgacggtt gccgcgcgcc gacggccacc cgtgccggcc gccacccgtg
     4741 ccggggacct cccggccggc cgggggcgaa tgcccggtga ggggcgtgag ttcggtggat
     4801 gggggtgcgt ctgcggccgg gtgcgggttc tcggtcgggt ggacggtgat gtgtcgggtt
     4861 cggaggtccc cgggcatccg ggtgtcgcgg tgaaactcgt cccggcggtg gcgttgtcct
     4921 cgggtcgtcc ggatgtatag cctaagcaat ggcatgtgac attgcactag aggcggtcgt
     4981 gcggacgccg aggggtggtc atggaactcg tcatcgtcgg tgcgggcatc gtgggcgccg
     5041 cgtgcgcgtt ccacgccgcc tccgccggtc tcggcgtcac ggtgctcgac cgggggcccg
     5101 tcggtgcggg caccaccagc cgcggcgagg ggaacatcct gctctccgac aaggagccgg
     5161 gcccggagct ggagctggcc cggctcagcc gtgacctctg gggcgaggcg agcgaggaac
     5221 tcggggcgcg gtccgtcgag ttcgagagca agggcggcct ggtcgcggcc agcacccccg
     5281 aaggcctcgc ggccctgcac gcgttcgccg cacgccaggc ggcggcgggt gtgcgcaccg
     5341 aactggtcac gtgtgcacgg gggttcgaac cgcacatcgc tcctggtctc cccggtgccg
     5401 tccactaccc gcaggacgcc caggtgcagc ctgtcctggc cgccgccgga ctgctgcaag
     5461 ccgccgtccg gcgcggcgcc cgggtccgta ccggcgaggc cgtcgccgcc gtcaccggaa
     5521 ccggcgggcg gatcaccgga gttcgcacgg ccgacggttc ggtgctggcc gccgacgccg
     5581 tggtcaacgc ggccgggacg tggggcggcg aggtcggccg ccgcctcggc gcgccggtcg
     5641 agatcctgcc gcgccggggg ttcgtcctcg tcaccgagcc gctgccgccg atgatccggc
     5701 acaaggtcta ttccgccgac tacgtcgcca acgtcgcctc caacggcgag ggcctggaga
     5761 cctcgtgcgt tgtcgagggc acccgcgcgg gcaccatcct gatcggcgcc agccgtgaac
     5821 gcgtcggctt cgacaccgcg atgaaccctg acgtggtggc cctgctcgcc gcgcaggcgt
     5881 gccgcctctt cccgtttctg cgcgacgtcc atctgatccg cgcctaccgg gggttcaggc
     5941 cgtactgccc cgaccatctg ccggtcgtcg gcccggaccc ccgggttccc ggtgtcctgc
     6001 acgcgtgcgg ccacgagggc gcgggcatcg gtctcgctcc cggaaccggc gccctcatca
     6061 ccgcccagtt gctcggccgc ccgtggcgcg gtgccgatcc ggccgcccac accggcctcc
     6121 tgcccgaccg attcctcacg accggaggtg tgccgaagtg accggagcgg ccgcggagac
     6181 cgcggcgacc gacgtaccca ccgtgaccgt ggacggcgaa cccctggcgt tcgtcccggg
     6241 gcagaccctg gccgccgcgc tcgtcgcctc gggccgggtc gcctggcgga ccacccgggg
     6301 cgggcagcgg ccccggggcg tcttctgcgg gatcggggtc tgttacgact gcctcgtcgc
     6361 cgtcgacgga cggcgcggac agcgcgcctg cctggtgcgg gcccgcgcgg gcatggccgt
     6421 cgcgacggag gagggcgacg atgactgagc cccgggacct cggcggcctc cccgcgatgc
     6481 aaaagccaca cgacctcgtg gtgctgggcg ccgggccggc cggtatggcg gccgccgcca
     6541 ccgccctgga cggtgggctg cgggtcgccc tcgtcgacgc gggccccgct cccggcggcc
     6601 agttctggcg ccaccccccg gaccacgcgc gcgacgccgt tcccaccgag gacctgcacc
     6661 acgacctgcg cacctaccgc gccctccgcg cgaccctggc cgcccacgag cgcaccggac
     6721 ggctcaccct cctgctcgac caccacgtgt ggaccacggc ccgcgcggtg gacggcttcg
     6781 ccgtccacgc cgtggaccgc ggcaggccgc ccgaggagcg ggccgtcgtg ctgcgcgcac
     6841 cggccctcct ggtggcgacc ggcgcctacg accgccaact ccccttcccc ggctgggacc
     6901 tgcccggcgt gctcaccgcg ggcggactcc agtcgctgct caagggcggg ggagtggtcg
     6961 ccggccgccg cgtggtcctc gggggtacgg gcccgttcct gctgcccgtc gccgccgccc
     7021 tcgccgcacg cggcgcggag gtcgtcgcgg tgtgcgaggc ggcggcgccc tcggcctggc
     7081 tgcggcaccc cgcacccctg ctgcgtaacc ccgccaaatg ggccgaagcc gctggatacg
     7141 cgggcacgct cgcccggcac cgcatcccgg tccgtacccg caccggcatc atcggcgccg
     7201 aaggggcggg gcgggtcgcc gtcgtacgca ccgcctccct gggcgccgac ggggcgccgc
     7261 tgccgggtac cgagcggcgg atcgaggccg acaccgtggg cgtcggctgg ggcttcgtcc
     7321 cccagctcga cctgctgctc ccgctcggct gcgacctcgc cgacgcgggc gacggcacca
     7381 cggccgccgc cgtcgacgcc ggacagcgca ccacggtgcc ggggctctac gcggcgggcg
     7441 agacctgcgg ggtgggcggg gcggcgctcg ccctgagcga agggcgcgtg gcggccgtct
     7501 ccgtgctcac cgacctctcc gcgccgggcc ggccgggcat ccggcccctg gccgcggagc
     7561 gccgatcggt ggcccggcac cgcgccttcg cccgcgccct ggcccaggcg caccccgtgc
     7621 cccggaactg gcccgcctgg ctgaccgacg acaccacggt ctgccggtgc gaggaggtca
     7681 ccgcgggggc ggtccgcgcc gcccgcgccg acggcccggc aaccgaccac cggcaggtca
     7741 aacaactgac cagggcgggc atggggtggt gccagggccg gatgtgcggg cccgccgtgc
     7801 actgtctcgt cgccgcccga gccgagtcct acacccccgc cgaacggctc atcgcgaccc
     7861 ccgtcaccct cggcgcgctc gccgactcca gcacgttccc caccggcgac acccctaccg
     7921 aacccacctg aggagttccc atgaaccacg tggaccgcac agcccctgcc cggatgccct
     7981 ggcacggcgt catcgtcgcg acgagcctcc cgttcgaccg ggacctcgcc gtcgaccacg
     8041 gcgcctacgg cgaccacgtc gcctggctcg ccgcccaggg cctgcacggt gtcgccccga
     8101 acggctcgct gggcgagtac cagaccctga cgtacgagga acgcgaccgt gtcgtcgaga
     8161 ccgccgtcgc ccacgccccc gagggcttca ccgtgatgcc gggcgtcggt gcgtacggcg
     8221 cgatcgaggc gcggcggcac gccctcttcg ccaaggatgc cggctgccag gccgtcatgt
     8281 gcctgccgcc caacgcctac cgggccgacg accgcgccgt cctggagcac tacgcgatgg
     8341 tggcctccgc cgggctcccc gtcaccgcct acaacaaccc catcgacacc aaggtcgacc
     8401 tgcgtcccga actgctggcc aagctgtacg ccgaggggtt catcgtggcc gtgaaggagt
     8461 tctccggaga cgtacgacgg tgttacgcca tcagtgaact ggcccccggc ctcgacctga
     8521 tcatcggcac cgacgacacc ctcctcgaag tcggcgtcgc cggggccaag ggctgggtcg
     8581 ccggatatcc gcaggtcttc ccgcgtgcct gcctcgacct ctacgatgcc gcactggccg
     8641 gcgacctcgc caccgcgctc ccgctctacc gccggctcca ctccgtactg cgctgggaca
     8701 gcaggaccga gttcgtccag gcgatcaagc tcggccagga catgatcggc cggaccggcg
     8761 gcgtctgccg gccgccccgc cggcccctgg acccggagac cgaggccgtc gtacgttccg
     8821 cgacccgggc gctcatcgac gcgggggtga actgacgtgc tttccacggt gtgttaccac
     8881 gccgtcgact cgcacaccga gggcatgccg acccgggtga tcaccggcgg ggtgggtgtc
     8941 ctgccgggcg cgaccatggc ggagcgccgg cagcggttca tggccgaacg cgacggactg
     9001 cgcaccctgt tgatgtgcga gccgcgcggg cacggggcca tgtccggcgc gatactccag
     9061 cccccgaccc ggccggacgc cgacttcggc gtgctgttca tcgaggtctc cggctgcctg
     9121 cccatgtgcg gccacggcac catcggcgtg gcgacggtcc tggtggagac cggcatggtc
     9181 gaggcggtcg agccggagac cctcatccgc ctcgacaccc ccgccggtct cgtcacggca
     9241 cgggtcgccg tgcgcgacgg ccgcgcggag tccgtcaccc tggagaacgt cgcctcctac
     9301 agccacgccc tggaccaggt cgtcgacgtg gcggggttcg gcccggtccg gtacgacatg
     9361 gcgtacggcg gcaacttcta cgcgatcgtc cgcaccgagg atctgggcat ccccttcgac
     9421 cgggcggaga agggccggct gctggacgcg ggcctcgccg tcatgcgggc ggtcaacgag
     9481 cagaacccgg tggtccaccc ggagaacccc gcgatcgacg tctgccacca cgtctacctg
     9541 gaggcccccg gctccaccgc cgaacactcg cggcacgcca tggcgatcca ccccggctgg
     9601 ttcgaccgct caccctgcgg gacgggaacc agcgcccgga tggcccaact gcacgcgagg
     9661 gggctgctga aaaccgggca ggacttcgtc aacgagtcct tcatcggctc ccgcttcacc
     9721 ggccggatcc tggacgagag cacggtcggc ggccgacccg ccgtcctgcc ctccgtcacc
     9781 ggtcgcgcct ggatcaccgg caccgcgcag tatctgctcg acccgaacga cccctacccg
     9841 gagggattca ccgtctgacg tccgctcggc acaccggccc cagggggccc gcgccgcgcg
     9901 aaacgccaac cctcgccgcc cggcaccgac ttcggctcag cgcgcaccga gcttgcggga
     9961 cggggccgcg cgctcggcct cccgcttccg tgccgcccac agggtccgca catgcgcgag
    10021 atggcggcgc atgtgctcct cggccgcctc cgcgtcgccc tcgatcatga tgtccaggag
    10081 cgcggtgtgc tcccgcgccg aacccaccaa cgacccttcc tgggacaggt cggtgagtcc
    10141 gtacagccgg gagcgcttgc gcaggtccgc caccgtctcg acgagacggg cgttacccgc
    10201 gagggcgagc aggtcgagat ggaagcggcg gtccgactcc aggtagccga tcaggtcgcc
    10261 ccgctgcgcg gccgccacga tctcctcggc caccgggcgc accgcctcca actgctcgcg
    10321 ggaggcggtg cgggtcaccc ggccgatggt ggggatctcg atgagcgcgc ggatctcggt
    10381 gtactcgtcc aggtcgcgct cggagagctc ggtgacccgg aagcccttgt tgcgcaccgc
    10441 ctcgaccagg ccctcgcggg ccagatccag catcgcctcg cgcacgggtg tggccgagac
    10501 gccgtactcg gcggccaggg tgggcgccga gtagatcgcg ccgggctgga gttctcccgc
    10561 tatcagcgcg gcacggaggg cgcccgccac ctggtcgcgc agatgctcct gcaccgagat
    10621 gagtttgcgg gacttcagct cactcatgcg ttccccttca gatttgtcac cgcactatac
    10681 aatgtcacgt tgcgcggtgc gccccctcct cggtcggcag ctggcagccg tcagtcgtcc
    10741 aggaggcggg ccatccactc ctcgacgccg tcggccgtac ggggcagcgc tccggagagg
    10801 agcttcgcac catcggcggt gatcacgagg tcgtcctcga tgcgcacgcc catgccacgc
    10861 agttcgggcg gcagcgtctc gtcgtccggc tggagataga gcccgggttc gacggtgagg
    10921 acatggccct cctccagtac tccgtcgaga taggtctcca cccgggcccg ggcgcagtcg
    10981 tgcacgtcga tgccgagcat gtgcccgctg ctgcacaggg tgtagcggcg gtacaggccg
    11041 ctgtcgtcgg cgagcgcctc ctcgggtgcg accggcagga cgccccaggc gtgcagcccc
    11101 tcggcgatga cgcgcatggc ctcccggtgg aagtcccgga agcgggctcc gggccgcagg
    11161 acggcgatgg cggcctcctg tgcggccagc accagttcgt agacgtcgcg ttgggccggt
    11221 gagaagcggc ccgacagggg gagggtgcgg gtgatgtccg cggtgtagag ggtgtcggtc
    11281 tccacccccg cgtcgagcag gaggagttgg gacggcgaga gcgggccgtc attgcggatc
    11341 cagtgcagga cgcaggcgtg cgcacccgcc gccgcgatgg tctcgtagcc cgtgccgttg
    11401 ccctcggtcc gtgcgcgcag gttgaacacg ccttcgatcc agcgttcgcc gcgggggtgg
    11461 cgcagggcgt cgggcaggga gcgcgccacg tcctcgaagc ccgacgtggt gtggtcgacg
    11521 gccagttgga gctggctcac ctcccacggg tccttgagca agcggagttc ggcgagcaca
    11581 gagtccaact cggcgtcccc gaaaggggtg tgggaccgcg cgggcagcag gtcgtcgatg
    11641 tggggatcga cgcccgcgag caggcgggtc ggcggctgag gaccggcgag gagccttggc
    11701 agcccgtcca ggtgggcgca gcgcagcccg gtcagcgccg ccgtctcgtc caggtcgggc
    11761 cggcggccga cccagaactc gccgtagcgg cggtcgcggt agaacccgct ggtgtcccgg
    11821 ggggagcggg ggcggatgtg gagcaccgcc tcgtggccgt cggggcccga cggttcgagg
    11881 accaggacgt ggtcgggctg ctcctcaccg gtcaggccgg tcagccaggc gtaggcgctg
    11941 tgcgggcgga agcggtgatc ggtgtcgttc gagcggacct tgagcgttcc ggcgggtatg
    12001 accagccgtt cgcccgggaa gcgcgccgac aggcgctgcc gccggccggg gagcagggcg
    12061 tggccgggga cccgtgcgtc ggcgggcagc ggggagggcg cccagccggt ggccatgaag
    12121 gcgtccaggg cggccgggac cggcagatcg tggctgcccg tgtgcagtcg ggccgggggc
    12181 gtggaggtca tgggtctcct tcggagggtg tgccttgggg gtgccggggc tgccaggggt
    12241 gccgggacgg ggagccgaag cgtccgactg cgggacacgt ttcggtaacg gggcgacgca
    12301 ctccttggct gtgcaatttc acattactat gttacgggtc acatcgcgtg gatggatgcg
    12361 ttcccggccg ctccgcctcg cgttccgcaa acggccgccc accgggccgc tcggccgccc
    12421 ggagcaccag atcccgccgg acccctcctt cttcctcctt tccgccggcc cgttcctctg
    12481 tcgccgtgcc ccgttcccgt accgaggagt gccccatgtc ccgtcgcagc cgtgcgctgt
    12541 ccgtatccct gagcacctcg ctggccctgg ccctgctggg cgcctgctcc ccgcagggag
    12601 gtgacgaccg aggcccggac gcgcggcagc ccggtgtggc gaccggcacc gtcatcggcg
    12661 gcacaccgaa gcggggcggc accctgaccg tgctgtccaa ccaggacttc gcccatctgg
    12721 acccggcccg caactgggtc atgccgacca tggacttcgg cacccggctg atctaccgca
    12781 ccctcacgac cttccgggca gcgcccggga agcggggcag cgaggtcgtc cccgatctcg
    12841 cgaccgacct cggccgatcc tccgacgggg gacgcacctg gaccttcacc ctgaagcccg
    12901 gcctcgtcta cgaggacggc accccgatcc g
//
