LOCUS       NZ_BMSI01000054         1501 bp    DNA     linear   CON 02-AUG-2024
DEFINITION  Streptomyces asoensis strain JCM 4185 sequence54, whole genome
            shotgun sequence.
ACCESSION   NZ_BMSI01000054
VERSION     NZ_BMSI01000054.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces asoensis
  ORGANISM  Streptomyces asoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649075.1-RS_2024_08_02
            Annotation Date                   :: 08/02/2024 04:45:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,813
            CDSs (total)                      :: 7,736
            Genes (coding)                    :: 7,563
            CDSs (with protein)               :: 7,563
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 53 of 173
            Pseudo Genes (incomplete)         :: 140 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 28 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:21:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 1501
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSI01000054.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..1501
                     /culture_collection="JCM:4185"
                     /db_xref="taxon:249586"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces asoensis"
                     /strain="JCM 4185"
                     /submitter_seqid="sequence54"
                     /type_material="type strain of Streptomyces cacaoi subsp.
                     asoensis"
     gene            <1..>1501
                     /locus_tag="IE189_RS38685"
     region          1..1501
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..1501
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..1501
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[2:>1501](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3..>1501
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     aSDomain        4..984
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38685_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="4.20E-140"
                     /label="IE189_RS38685_PKS_KS.1"
                     /locus_tag="IE189_RS38685"
                     /protein_end="327"
                     /protein_start="0"
                     /score="459.3"
                     /tool="antismash"
                     /translation="MERAGIDPGSLKGSATGVFAGVMHHDYASAAGQPEELEGYAVTST
                     QGSVASGRLAYTFGLEGPAVTVDTACSSSLVALHLAVQALRSGECTVALAGGVSVMATP
                     WVFVEFSRQRGMAPDGRCKAFAGAADGAGWSEGVGLLLVERLADARRNGHPVLAVVRGS
                     AVNQDGASNGLTAPNGPSQQRVIRAALANARLTPADVDVVEAHGTGTTLGDPIEAQALL
                     ATYGQDRPAGRPLRLGSVKSNIGHTQAAAGAAGVIKMVEAMRHGVLPRTLHVDEPTPHV
                     DWSAGAVELLTDSVEWTTDGTRPLRAGVSSFGISGTNAHVILEEAP"
     CDS             3..>1501
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (0-327). E-value:
                     4.2e-140. Score: 459.3. Matches aSDomain:
                     nrpspksdomains_IE189_RS38685_PKS_KS.1"
                     /codon_start=2
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014173981.1"
                     /locus_tag="IE189_RS38685"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_189928755.1"
                     /sec_met_domain="mod_KS (E-value: 2.3e-176, bitscore:
                     572.8, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 6.6e-54, bitscore:
                     169.2, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 7.6e-20, bitscore: 57.5,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERAGIDPGSLKGSATGVFAGVMHHDYASAAGQPEELEGYAVTST
                     QGSVASGRLAYTFGLEGPAVTVDTACSSSLVALHLAVQALRSGECTVALAGGVSVMATP
                     WVFVEFSRQRGMAPDGRCKAFAGAADGAGWSEGVGLLLVERLADARRNGHPVLAVVRGS
                     AVNQDGASNGLTAPNGPSQQRVIRAALANARLTPADVDVVEAHGTGTTLGDPIEAQALL
                     ATYGQDRPAGRPLRLGSVKSNIGHTQAAAGAAGVIKMVEAMRHGVLPRTLHVDEPTPHV
                     DWSAGAVELLTDSVEWTTDGTRPLRAGVSSFGISGTNAHVILEEAPEAEEPEESSGDPR
                     LVADVLPWVVSGRSDAALRAQAGRLAGFVEQRSELTVADVARSLVETRSALEHRAVVLV
                     ADRDSAVGVLRSVAEGVPSAGVVSGVVSGEQGRVVFVFPGQGSQWLGMGAGLWESSPVF
                     RERLQECDAALSSLVDWSVTEVVRGVPDAASLDDVVVVQASL"
     CDS_motif       187..234
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38685_0001"
                     /evalue="1.90E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE189_RS38685"
                     /protein_end="77"
                     /protein_start="61"
                     /score="22.7"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       610..654
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38685_0002"
                     /evalue="7.10E-05"
                     /label="PKSI-KS_m5"
                     /locus_tag="IE189_RS38685"
                     /protein_end="217"
                     /protein_start="202"
                     /score="14.6"
                     /tool="antismash"
                     /translation="VEAHGTGTTLGDPIE"
     CDS_motif       715..759
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38685_0003"
                     /evalue="1.30E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="IE189_RS38685"
                     /protein_end="252"
                     /protein_start="237"
                     /score="14.0"
                     /tool="antismash"
                     /translation="GSVKSNIGHTQAAAG"
     CDS_motif       1303..1356
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38685_0004"
                     /evalue="1.30E-08"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="IE189_RS38685"
                     /protein_end="451"
                     /protein_start="433"
                     /score="26.6"
                     /tool="antismash"
                     /translation="VFVFPGQGSQWLGMGAGL"
ORIGIN
        1 gttcgagcgg gcgggtattg atccggggtc gttgaagggg agtgcgacgg gggtctttgc
       61 gggcgtcatg caccacgact acgcctccgc cgccggacag cccgaggaac tggagggcta
      121 cgccgtcacc agcacccagg gcagcgtcgc gtccggccgc ctggcctata cgttcggcct
      181 ggagggtccg gcggtgacgg tggacacggc gtgctcgtcg tcgctggtgg cgctgcatct
      241 ggccgtgcag gccctgcgct ccggcgagtg cacggtggcc ctcgccggcg gcgtcagcgt
      301 gatggccacg ccttgggtgt tcgtggagtt cagccgtcag cggggcatgg ctccggacgg
      361 gcggtgcaag gcgttcgcgg gggcggccga cggcgccggg tggtccgagg gcgtcggcct
      421 gctgctggtg gaacgacttg cggacgcgcg ccgcaacggg catccggtgc tggcggtggt
      481 ccggggctcg gcggtgaacc aggacggtgc gagcaacggc ctgacggcac ccaacggccc
      541 gtcccagcag cgcgtcatcc gcgcggcgct ggccaacgcg cggctcaccc cggcggacgt
      601 cgacgtcgtc gaggcccacg gcacgggcac cacgctcggt gacccgatcg aggcccaggc
      661 cctgctggcg acctacggcc aggaccgtcc tgccggccgg ccgctgcgac tgggctcggt
      721 gaagtccaac atcggccaca cccaggcggc ggcgggtgcg gccggggtga tcaagatggt
      781 ggaggcgatg cggcacggcg tcctgcccag gaccctgcac gtcgacgaac cgacaccgca
      841 cgtggactgg tcggcgggtg ccgtggaact gctgacggac tccgtcgaat ggacgacgga
      901 cggcacacgc ccgctccggg ccggtgtgtc ctcgttcggc atcagcggga ccaacgcgca
      961 cgtgatcctg gaggaggcgc cggaggcgga ggagccggaa gagtcttcgg gtgatccgcg
     1021 actcgtggcg gacgtgctgc cgtgggtggt gtcgggtcgt tcggacgcgg cgttgcgggc
     1081 gcaggcgggg cggctcgccg ggttcgtcga gcagcgctcc gagctgacgg tcgccgatgt
     1141 ggcgcgttcc ctggtggaga cgcggtcggc tctggagcac cgtgccgtgg ttctggtcgc
     1201 tgatcgggac tctgctgttg gggtgttgcg gtcggtggcg gagggtgtgc cttcggccgg
     1261 ggtggtgtcg ggtgtggtgt ccggggagca gggccgggtg gtgttcgtgt tcccgggcca
     1321 gggctcgcag tggctcggga tgggtgcggg gttgtgggag tcgtcgccgg tgttccggga
     1381 gcggcttcag gagtgtgatg cggccctgtc ttcgctggtg gactggtcgg tgaccgaggt
     1441 cgtccggggt gtgccggatg cggcttcgct ggacgatgtg gtggttgttc aggcgtcgtt
     1501 g
//
