LOCUS       NZ_BMSI01000043         2547 bp    DNA     linear   CON 02-AUG-2024
DEFINITION  Streptomyces asoensis strain JCM 4185 sequence43, whole genome
            shotgun sequence.
ACCESSION   NZ_BMSI01000043
VERSION     NZ_BMSI01000043.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces asoensis
  ORGANISM  Streptomyces asoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649075.1-RS_2024_08_02
            Annotation Date                   :: 08/02/2024 04:45:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,813
            CDSs (total)                      :: 7,736
            Genes (coding)                    :: 7,563
            CDSs (with protein)               :: 7,563
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 53 of 173
            Pseudo Genes (incomplete)         :: 140 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 28 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:21:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2547
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSI01000043.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..460
                     /locus_tag="IE189_RS38620"
     source          1..2547
                     /culture_collection="JCM:4185"
                     /db_xref="taxon:249586"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces asoensis"
                     /strain="JCM 4185"
                     /submitter_seqid="sequence43"
                     /type_material="type strain of Streptomyces cacaoi subsp.
                     asoensis"
     region          1..2547
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..2547
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2547
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[538:>2547](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      539..>2547
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS             <3..460
                     /NRPS_PKS="Domain: ACP (0-69). E-value: 4.9e-35. Score:
                     111.7. Matches aSDomain:
                     nrpspksdomains_IE189_RS38620_ACP.1"
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (81-151). E-value:
                     1.6e-22. Score: 72.1. Matches aSDomain:
                     nrpspksdomains_IE189_RS38620_PKS_Docking_Cterm.1"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091594.1"
                     /locus_tag="IE189_RS38620"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="acyl carrier protein"
                     /protein_id="WP_229901631.1"
                     /sec_met_domain="PP-binding (E-value: 3.6e-18, bitscore:
                     53.1, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="RAHVATVLGHGSPAAVDPGRAFKELGFDSLTAVELRNRLQAATGL
                     RLPATLVFDHPTPETLAEHLLAELSPEEDTAHGTESLLAELARLESAFAAVSADEAGHS
                     TVARRLENLLAAWKETGPAQRKETAGDRLKSASADEVLDFINSELGIS"
     aSDomain        5..211
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38620_ACP.1"
                     /evalue="4.90E-35"
                     /label="IE189_RS38620_ACP.1"
                     /locus_tag="IE189_RS38620"
                     /protein_end="69"
                     /protein_start="0"
                     /score="111.7"
                     /tool="antismash"
                     /translation="RAHVATVLGHGSPAAVDPGRAFKELGFDSLTAVELRNRLQAATGL
                     RLPATLVFDHPTPETLAEHLLAEL"
     aSDomain        248..457
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38620_PKS_Docking_Cterm.
                     1"
                     /evalue="1.60E-22"
                     /label="IE189_RS38620_PKS_Docking_Cterm.1"
                     /locus_tag="IE189_RS38620"
                     /protein_end="151"
                     /protein_start="81"
                     /score="72.1"
                     /tool="antismash"
                     /translation="LLAELARLESAFAAVSADEAGHSTVARRLENLLAAWKETGPAQRK
                     ETAGDRLKSASADEVLDFINSELGI"
     gene            539..>2547
                     /locus_tag="IE189_RS38625"
     CDS             539..>2547
                     /NRPS_PKS="Domain: PKS_Docking_Nterm (3-31). E-value:
                     5.6e-15. Score: 46.6. Matches aSDomain:
                     nrpspksdomains_IE189_RS38625_PKS_Docking_Nterm.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (35-461). E-value:
                     1.1e-180. Score: 593.0. Matches aSDomain:
                     nrpspksdomains_IE189_RS38625_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014173985.1"
                     /locus_tag="IE189_RS38625"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_189928749.1"
                     /sec_met_domain="mod_KS (E-value: 1.7e-230, bitscore:
                     752.2, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 1.4e-96, bitscore:
                     310.0, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 4.6e-18, bitscore: 52.7,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTNDEKLVDYLKRVAADLYETRQRLREVEEREHEPIAIVGVACRF
                     PGGVSSPEDLWRLVESGTDAISDFPDDRGWDVEDLYDPDPDVVGKTYLRQGGFLHGAGD
                     FDADLFGISPREALAMDPQQRLVLESSWEVLERAGIDPTSLKGTKTGVFVGAAGTGYLG
                     TMQRVPDGVEGYTGVGSFVSVISGRVSYTLGLEGPAITVDTACSSSLVALHMAVQSLRS
                     GECSLAVAGGVAVMPTPWVFVDFSRQRGLARDGRCKAFAAAADGTAWSEGVGTLLVERL
                     SDARRNGHRVLAVVRGSALNQDGASNGLTAPNGPSQQRVIRAALANAQLSADEVDAVEA
                     HGTGTPLGDPIEAQALVATYGRNRPADRPLWLGSVKSNLGHTAHAAGMAGVIKMVMALH
                     NETLPRTLHVDAPSPHVDWTSNTVRLLTDQVEWARNGHPRRVGVSSFGISGTNAHLIIE
                     EAPQPETEDPGPGAPQPGAHQGESTVGAETGTETGTETGAETGSAPGTRTGAPTDGEST
                     SRPLTALPAVPWVVTGRTADALRGQAARLAAFVEDTPADLTDLGFSLVASRAALEHRAV
                     ITAADRQGALTALTALAQGVPAVDAVSGAVLSGAPRVVFVFPGQGSQWVGMGAGLWESS
                     PVFRERLQECDAALSVLVDWSVTDVVRGVPDAASL"
     aSDomain        548..631
                     /aSDomain="PKS_Docking_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38625_PKS_Docking_Nterm.
                     1"
                     /evalue="5.60E-15"
                     /label="IE189_RS38625_PKS_Docking_Nterm.1"
                     /locus_tag="IE189_RS38625"
                     /protein_end="31"
                     /protein_start="3"
                     /score="46.6"
                     /tool="antismash"
                     /translation="DEKLVDYLKRVAADLYETRQRLREVEER"
     aSDomain        644..1921
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38625_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="1.10E-180"
                     /label="IE189_RS38625_PKS_KS.1"
                     /locus_tag="IE189_RS38625"
                     /protein_end="461"
                     /protein_start="35"
                     /score="593.0"
                     /tool="antismash"
                     /translation="IAIVGVACRFPGGVSSPEDLWRLVESGTDAISDFPDDRGWDVEDL
                     YDPDPDVVGKTYLRQGGFLHGAGDFDADLFGISPREALAMDPQQRLVLESSWEVLERAG
                     IDPTSLKGTKTGVFVGAAGTGYLGTMQRVPDGVEGYTGVGSFVSVISGRVSYTLGLEGP
                     AITVDTACSSSLVALHMAVQSLRSGECSLAVAGGVAVMPTPWVFVDFSRQRGLARDGRC
                     KAFAAAADGTAWSEGVGTLLVERLSDARRNGHRVLAVVRGSALNQDGASNGLTAPNGPS
                     QQRVIRAALANAQLSADEVDAVEAHGTGTPLGDPIEAQALVATYGRNRPADRPLWLGSV
                     KSNLGHTAHAAGMAGVIKMVMALHNETLPRTLHVDAPSPHVDWTSNTVRLLTDQVEWAR
                     NGHPRRVGVSSFGISGTNAHLIIEEAP"
     CDS_motif       1127..1174
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38625_0001"
                     /evalue="2.90E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE189_RS38625"
                     /protein_end="212"
                     /protein_start="196"
                     /score="22.2"
                     /tool="antismash"
                     /translation="GPAITVDTACSSSLVA"
     CDS_motif       1550..1594
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38625_0002"
                     /evalue="1.40E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="IE189_RS38625"
                     /protein_end="352"
                     /protein_start="337"
                     /score="13.8"
                     /tool="antismash"
                     /translation="VEAHGTGTPLGDPIE"
     CDS_motif       1655..1699
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38625_0003"
                     /evalue="3.60E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="IE189_RS38625"
                     /protein_end="387"
                     /protein_start="372"
                     /score="10.1"
                     /tool="antismash"
                     /translation="GSVKSNLGHTAHAAG"
     CDS_motif       2378..2431
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38625_0004"
                     /evalue="1.20E-08"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="IE189_RS38625"
                     /protein_end="631"
                     /protein_start="613"
                     /score="26.7"
                     /tool="antismash"
                     /translation="VFVFPGQGSQWVGMGAGL"
ORIGIN
        1 gcgtgctcat gtcgccacgg tgctggggca tggttcaccg gcggcggtgg atcccggacg
       61 ggcgttcaag gagctgggct tcgactcgct gaccgccgtc gaactgcgca accggcttca
      121 ggccgcgacc ggcctgcgac tgcccgccac cctcgtcttc gaccacccca cccccgaaac
      181 cctcgccgag catctccttg cggagctgtc gcccgaggaa gacacggccc atgggaccga
      241 gtccctgctc gccgaactgg cccggctcga atcggccttc gccgcggtct ccgcggacga
      301 ggccggacat tccaccgtcg cacggcgcct ggagaacctg ctcgcggcct ggaaggaaac
      361 agggccggcg cagcgcaagg aaacagccgg tgatcgtctc aagtcggcat ccgctgacga
      421 agtcttggat ttcatcaaca gcgagctcgg gatttcctga acggtcacga tcccgcccgc
      481 ggacaagccg ttcgcgccat tccggaattc ccgaacgctc gaaggaggac cgcgtcagat
      541 gacgaacgac gagaagctgg tcgactacct gaagcgcgtg gcggccgacc tctatgagac
      601 gaggcagcgc ctgcgcgagg tcgaggagcg cgagcacgag cccatcgcca tcgtcggcgt
      661 cgcctgccgc ttccccggag gggtgagttc gcccgaggac ctgtggcggc tcgtggaatc
      721 cggcaccgac gcgatctccg acttccccga cgaccgtggc tgggacgtcg aggacctcta
      781 cgaccccgac cccgacgtcg tcggcaagac ctatctgcga cagggcggtt tcctgcacgg
      841 cgccggtgac ttcgacgccg acctcttcgg catatcgccg cgcgaggccc tcgcgatgga
      901 cccgcagcag cggctggtcc tggagtcctc ctgggaggtg ctggaacgcg ccggcatcga
      961 cccgacgtcg ctcaagggca cgaagaccgg cgtgttcgtc ggcgccgccg gcaccggcta
     1021 cctcggcacc atgcagcgcg tgcccgacgg ggtggagggc tacaccggcg tcggctcctt
     1081 cgtcagcgtc atctccggcc gggtctccta cacgctcggg ctggaagggc cggcgatcac
     1141 cgtcgacacg gcctgctcct cttccctggt cgccctgcac atggccgtgc agtcgctgcg
     1201 ctcgggcgag tgctcgcttg ccgtggccgg cggcgtcgcc gtcatgccca ccccctgggt
     1261 cttcgtggac ttcagccgcc agcgcggtct ggcccgcgac ggccgctgca aggcgttcgc
     1321 cgccgcggcg gacggcaccg cctggtcgga gggcgtgggc accctcctgg tcgaacgtct
     1381 ctccgacgcc cggcgcaacg gccaccgggt cctcgccgtc gtccgcggtt ccgcactgaa
     1441 ccaggacggc gcgagcaacg gcctcaccgc ccccaacggc ccctcccagc aacgcgtcat
     1501 ccgcgccgcc ctcgccaacg cgcagctgtc cgccgacgag gtggacgcgg tcgaggcgca
     1561 cggcaccggc acccccctcg gcgaccccat cgaggcccag gccctggtcg cgacctacgg
     1621 caggaaccgc cccgccgacc gcccgctgtg gctcgggtcc gtgaagtcca acctcgggca
     1681 caccgcccac gcggcgggta tggccggcgt catcaagatg gtcatggcgc tgcacaacga
     1741 aacgctgccg cgcaccctgc acgtggacgc gccctcaccg cacgtggact ggacctcgaa
     1801 caccgtccgg ctgctcaccg accaggtgga gtgggcacgc aacggccacc ccaggcgcgt
     1861 cggcgtgtcc tcgttcggca tcagcggcac caacgcccac ctgatcatcg aggaggcgcc
     1921 ccagccggag accgaagacc cggggcccgg cgcaccgcag ccgggcgcgc accagggcga
     1981 gtccaccgtc ggcgcggaga ccggcacgga gaccggcacg gagaccggcg cagagaccgg
     2041 ctcggcgccc ggcacacgga ccggcgcccc gacggacggc gagtcgacgt cccggccgct
     2101 gaccgccctg cccgcggtcc cgtgggtcgt caccggccgc accgcggacg cgctgcgagg
     2161 ccaggccgca cgcctggccg ccttcgtgga ggacaccccg gcggacctca ccgatctcgg
     2221 cttctccctc gtcgcctccc gcgccgcact ggaacaccgc gccgtgatca cggccgcgga
     2281 ccgacagggc gcgctgaccg cgctgaccgc actcgcccag ggcgtccccg ccgtggacgc
     2341 ggtgtcgggt gcggtgctgt ccggggcgcc gcgggtggtg ttcgtgttcc cgggtcaggg
     2401 gtcgcagtgg gtggggatgg gtgcggggtt gtgggagtcg tcgccggtgt tccgggagcg
     2461 gctccaggag tgtgacgcgg cgctgtctgt gctggtggac tggtcggtga ctgatgtggt
     2521 gcggggtgtg ccggatgcgg cttcgct
//
