LOCUS       NZ_BMSI01000042         2547 bp    DNA     linear   CON 02-AUG-2024
DEFINITION  Streptomyces asoensis strain JCM 4185 sequence42, whole genome
            shotgun sequence.
ACCESSION   NZ_BMSI01000042
VERSION     NZ_BMSI01000042.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces asoensis
  ORGANISM  Streptomyces asoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649075.1-RS_2024_08_02
            Annotation Date                   :: 08/02/2024 04:45:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,813
            CDSs (total)                      :: 7,736
            Genes (coding)                    :: 7,563
            CDSs (with protein)               :: 7,563
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 53 of 173
            Pseudo Genes (incomplete)         :: 140 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 28 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:21:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 2547
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSI01000042.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..2547
                     /culture_collection="JCM:4185"
                     /db_xref="taxon:249586"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces asoensis"
                     /strain="JCM 4185"
                     /submitter_seqid="sequence42"
                     /type_material="type strain of Streptomyces cacaoi subsp.
                     asoensis"
     gene            <1..>2547
                     /locus_tag="IE189_RS38615"
     region          1..2547
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..2547
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..2547
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[0:>2547](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      1..>2547
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS             1..>2547
                     /NRPS_PKS="Domain: ACP (163-234). E-value: 1.7e-31. Score:
                     100.3. Matches aSDomain:
                     nrpspksdomains_IE189_RS38615_ACP.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (261-687). E-value:
                     5.3e-183. Score: 600.7. Matches aSDomain:
                     nrpspksdomains_IE189_RS38615_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1047: phosphopantetheine-binding domain-containing
                     protein"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009715042.1"
                     /locus_tag="IE189_RS38615"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_189928748.1"
                     /sec_met_domain="PP-binding (E-value: 2.4e-15, bitscore:
                     43.1, seeds: 164, tool: rule-based-clusters)"
                     /sec_met_domain="mod_KS (E-value: 3e-234, bitscore: 763.6,
                     seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 4.1e-96, bitscore:
                     307.5, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 3.9e-18, bitscore: 51.9,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDLSAFVLFSSVAGTLGNAGQANYAAANAFLDALASHRGASGLP
                     AHSLAWGLWEQSTGMLDHLDGDDLAWMRRAGIHPLPADLGLALLDTATALDDAHLVPVL
                     LDPATLRGEAASGMLRPLLRDLVRTGIRPRAAAATPADGSALAQRLAGLTEEQQEREVL
                     GLVRAQVATVLGHATPETVALGHPFKELGFDSLTALELRNRLNAVTGLRLPATLVFDYP
                     TPLVLAGFVRGEVLGLGAGVSAGVARSVVGGGGVGVDEPVAIVGMACRFPGGVGSPEGL
                     WDLVASGGDAVSLFPGGRGWDVEGLYDPDPGVVGKSYAREGGFLEGAGEFDAGFFGISP
                     REALAMDPQQRLLLETAWEVFERAGIDPGSLKGSATGVFAGVMHHDYASRVEQLQTETE
                     GYALMGASPSAVTGRVAYTFGLEGPAVTVDTACSSSLVALHLAVQALRSGECSMALAGG
                     VTVMSTPTLFVEFSRQRGMAPDGRCKAFAAAADGAGWSEGVGLLLVERLSDARRHGHRV
                     LAVVRGSAINQDGASNGLTAPNGPSQQRVIRAALANARLTPADVDVVEAHGTGTTLGDP
                     IEAQALLATYGQDRPAGRPLRLGSVKSNIGHTQAAAGVAGVIKMVEAMRHGVLPRTLHV
                     NEPSPHIDWSSGAVELLTDTVEWTTDGQRPRRAGISAFGASGTNAHLILEQEPEENTPP
                     ATEPAVSPVDALVVPWVLSGRTEGGLRGQAARLAAFADERTGVSLPDVGWSLATGRAAL
                     EHRAVVLAGDREAAVGVLRSVAEGVPSAGVVSGVVSGEQGRVVFVFPGQGSQWLGMGAG
                     LWESSPVFRERLQECDAALSSLVDWSVTDVVRGVPDA"
     CDS_motif       16..117
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38615_0001"
                     /evalue="3.00E-20"
                     /label="PKSI-KR_m4"
                     /locus_tag="IE189_RS38615"
                     /protein_end="39"
                     /protein_start="5"
                     /score="64.8"
                     /tool="antismash"
                     /translation="AFVLFSSVAGTLGNAGQANYAAANAFLDALASHR"
     aSDomain        490..702
                     /ASF="found active site serine: True"
                     /ASF="non-beta-branching ACP"
                     /aSDomain="ACP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38615_ACP.1"
                     /evalue="1.70E-31"
                     /label="IE189_RS38615_ACP.1"
                     /locus_tag="IE189_RS38615"
                     /protein_end="234"
                     /protein_start="163"
                     /score="100.3"
                     /tool="antismash"
                     /translation="GLVRAQVATVLGHATPETVALGHPFKELGFDSLTALELRNRLNAV
                     TGLRLPATLVFDYPTPLVLAGFVRGE"
     aSDomain        784..2061
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38615_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="5.30E-183"
                     /label="IE189_RS38615_PKS_KS.1"
                     /locus_tag="IE189_RS38615"
                     /protein_end="687"
                     /protein_start="261"
                     /score="600.7"
                     /tool="antismash"
                     /translation="VAIVGMACRFPGGVGSPEGLWDLVASGGDAVSLFPGGRGWDVEGL
                     YDPDPGVVGKSYAREGGFLEGAGEFDAGFFGISPREALAMDPQQRLLLETAWEVFERAG
                     IDPGSLKGSATGVFAGVMHHDYASRVEQLQTETEGYALMGASPSAVTGRVAYTFGLEGP
                     AVTVDTACSSSLVALHLAVQALRSGECSMALAGGVTVMSTPTLFVEFSRQRGMAPDGRC
                     KAFAAAADGAGWSEGVGLLLVERLSDARRHGHRVLAVVRGSAINQDGASNGLTAPNGPS
                     QQRVIRAALANARLTPADVDVVEAHGTGTTLGDPIEAQALLATYGQDRPAGRPLRLGSV
                     KSNIGHTQAAAGVAGVIKMVEAMRHGVLPRTLHVNEPSPHIDWSSGAVELLTDTVEWTT
                     DGQRPRRAGISAFGASGTNAHLILEQE"
     CDS_motif       880..912
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38615_0002"
                     /evalue="4.80E+01"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="IE189_RS38615"
                     /protein_end="304"
                     /protein_start="293"
                     /score="-2.2"
                     /tool="antismash"
                     /translation="LFPGGRGWDVE"
     CDS_motif       1039..1065
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38615_0003"
                     /evalue="1.20E-02"
                     /label="PKSI-KS_m2"
                     /locus_tag="IE189_RS38615"
                     /protein_end="355"
                     /protein_start="346"
                     /score="8.7"
                     /tool="antismash"
                     /translation="DPQQRLLLE"
     CDS_motif       1267..1314
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38615_0004"
                     /evalue="3.70E-07"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE189_RS38615"
                     /protein_end="438"
                     /protein_start="422"
                     /score="21.9"
                     /tool="antismash"
                     /translation="GPAVTVDTACSSSLVA"
     CDS_motif       1690..1734
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38615_0005"
                     /evalue="1.40E-04"
                     /label="PKSI-KS_m5"
                     /locus_tag="IE189_RS38615"
                     /protein_end="578"
                     /protein_start="563"
                     /score="13.8"
                     /tool="antismash"
                     /translation="VEAHGTGTTLGDPIE"
     CDS_motif       1795..1839
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38615_0006"
                     /evalue="2.60E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="IE189_RS38615"
                     /protein_end="613"
                     /protein_start="598"
                     /score="13.2"
                     /tool="antismash"
                     /translation="GSVKSNIGHTQAAAG"
     CDS_motif       2386..2439
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38615_0007"
                     /evalue="2.40E-08"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="IE189_RS38615"
                     /protein_end="813"
                     /protein_start="795"
                     /score="25.8"
                     /tool="antismash"
                     /translation="VFVFPGQGSQWLGMGAGL"
ORIGIN
        1 gagttggacc tgtcggcgtt cgttctcttc tcctccgtcg ccggcaccct cgggaacgcg
       61 ggccaggcca actacgccgc cgccaacgcg ttcctcgacg ccctggcctc ccaccgcggg
      121 gcgtccggcc ttcccgcgca ctccctcgcg tggggcctgt gggagcagtc caccgggatg
      181 ctcgaccacc tggacggcga cgacctggca tggatgcgcc gtgcgggcat ccaccccctg
      241 cccgccgacc tcggcctcgc actcctcgac accgcgaccg ccctggacga cgcccacctg
      301 gtgccggttc tcctcgaccc ggccaccctg cgcggcgagg ccgcctccgg gatgctgcga
      361 ccgctcctcc gcgacctggt gcgcaccggc atccgccccc gtgcggcagc ggcgaccccg
      421 gccgacggct ccgccctggc gcagcgcctc gccggcctca ccgaggaaca gcaggaacgc
      481 gaggtcctgg gactcgtacg ggcccaggtg gccacggtcc tcggacacgc aaccccggag
      541 accgtcgccc tcgggcaccc gttcaaggag ttgggttttg attcgttgac ggcgttggag
      601 ttgcggaatc ggttgaacgc ggtgacgggg ttgcggcttc cggcgacgtt ggtgttcgac
      661 tatccgacgc cgttggtgtt ggcggggttt gtgcggggtg aggtgctggg tctgggggcg
      721 ggtgtgtctg cgggtgtggc gcgttcggtg gtcggtggtg gtggtgtggg ggtggatgag
      781 ccggtggcga ttgtggggat ggcgtgtcgt tttccgggtg gggtggggtc gccggagggg
      841 ttgtgggatc tggtggcgtc gggtggggat gcggtgtcgt tgttcccggg gggtcgtggg
      901 tgggatgtgg aggggttgta cgacccggat ccgggtgtgg tggggaagtc gtatgcgcgt
      961 gagggtgggt ttttggaggg ggcgggggag ttcgatgcgg ggttcttcgg gatttctccg
     1021 cgtgaggcgt tggcgatgga tccgcagcag cggttgttgt tggagacggc gtgggaggtg
     1081 ttcgagcggg cgggtattga tccggggtcg ttgaagggga gtgcgacggg ggtcttcgcg
     1141 ggcgtcatgc accacgacta cgcctcccgg gtcgaacagt tgcagaccga gaccgagggg
     1201 tacgccctga tgggcgcctc ccccagcgcc gtgaccggcc gtgtcgccta cacgttcggc
     1261 ctggagggtc cggcggtgac ggtggacacg gcgtgctcgt cgtcgctggt ggcgctgcat
     1321 ctggccgtgc aggccctgcg ctccggcgag tgctccatgg ccctcgccgg cggtgtgacc
     1381 gtcatgtcga cgccgaccct cttcgtcgag ttcagccgtc agcgcggcat ggctccggac
     1441 gggcggtgca aggcgttcgc ggcggcggcc gacggcgccg ggtggtccga gggcgtcggc
     1501 ctgctgctcg tggaacggct ctcggacgcc cggcgccatg ggcaccgggt cctggccgtg
     1561 gtgcgtggca gcgcgatcaa ccaggacggt gcgagcaacg gcctgacggc acccaacggc
     1621 ccgtcccagc agcgcgtcat ccgcgcggcg ctggccaacg cgcggctcac cccggcggac
     1681 gtcgacgtgg tcgaggccca cggcacgggc accacgctcg gtgacccgat cgaggcccag
     1741 gccctgctgg cgacctacgg ccaggaccgt cctgccggcc ggccgctgcg actgggctcg
     1801 gtgaagtcca acatcggcca cacgcaggcc gccgcgggcg tagcgggcgt gatcaagatg
     1861 gtggaggcca tgcgtcacgg cgtcctgccc aggaccctgc acgtgaacga accctccccg
     1921 cacatcgact ggtcctccgg tgccgtggaa ctgctgacgg acacagtcga gtggacgacg
     1981 gacgggcaac gtccgcgtcg cgccgggatc tcggcgttcg gcgccagcgg gaccaacgcc
     2041 cacctcatcc tggagcagga gcccgaggag aacaccccgc ctgctacgga gcccgctgtc
     2101 tccccggtgg acgcactggt cgtgccctgg gtgctctccg ggaggaccga aggtggcctg
     2161 cggggtcagg cggcgcggct cgccgcgttc gcggacgagc gtacgggcgt gtcgctgccg
     2221 gacgtgggtt ggtcgctggc gacgggccgg gccgcgctcg agcaccgtgc ggtggttctg
     2281 gccggtgatc gggaggctgc ggttggggtg ttgcggtcgg tggcggaggg tgtgccttcg
     2341 gccggggtgg tgtcgggtgt ggtgtccggg gagcagggcc gggtggtgtt cgtgttcccg
     2401 ggccaggggt cccagtggct cgggatgggt gcggggttgt gggagtcgtc gccggtgttc
     2461 cgggagcggc ttcaggagtg tgatgcggcc ctgtcttcgc tggtggactg gtcggtgact
     2521 gatgtggtgc ggggtgtgcc ggatgcg
//
