LOCUS       NZ_BMSI01000040         3224 bp    DNA     linear   CON 02-AUG-2024
DEFINITION  Streptomyces asoensis strain JCM 4185 sequence40, whole genome
            shotgun sequence.
ACCESSION   NZ_BMSI01000040
VERSION     NZ_BMSI01000040.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces asoensis
  ORGANISM  Streptomyces asoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649075.1-RS_2024_08_02
            Annotation Date                   :: 08/02/2024 04:45:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,813
            CDSs (total)                      :: 7,736
            Genes (coding)                    :: 7,563
            CDSs (with protein)               :: 7,563
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 53 of 173
            Pseudo Genes (incomplete)         :: 140 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 28 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:21:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 3224
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSI01000040.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..306
                     /locus_tag="IE189_RS38600"
                     /pseudo=""
     CDS             <1..306
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (31-101). E-value:
                     1.5e-20. Score: 65.8. Matches aSDomain:
                     nrpspksdomains_IE189_RS38600_PKS_Docking_Cterm.1"
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE189_RS38600"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /product="polyketide synthase"
                     /pseudo=""
                     /transl_table=11
                     /translation="TVVFDYPNPLSLARHLHRELGTTTQPVVATHPLLAELSRLDAALA
                     TTPADDSVRAQVTARLQGLLASWSLANGTPPAEEELDFDAASDDELFDLIDTEFGN"
     source          1..3224
                     /culture_collection="JCM:4185"
                     /db_xref="taxon:249586"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces asoensis"
                     /strain="JCM 4185"
                     /submitter_seqid="sequence40"
                     /type_material="type strain of Streptomyces cacaoi subsp.
                     asoensis"
     region          1..3224
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..3224
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..3224
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[344:>3224](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      345..>3224
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     aSDomain        97..306
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38600_PKS_Docking_Cterm.
                     1"
                     /evalue="1.50E-20"
                     /label="IE189_RS38600_PKS_Docking_Cterm.1"
                     /locus_tag="IE189_RS38600"
                     /protein_end="101"
                     /protein_start="31"
                     /score="65.8"
                     /tool="antismash"
                     /translation="PLLAELSRLDAALATTPADDSVRAQVTARLQGLLASWSLANGTPP
                     AEEELDFDAASDDELFDLIDTEFGN"
     gene            345..>3224
                     /locus_tag="IE189_RS38605"
     CDS             345..>3224
                     /NRPS_PKS="Domain: PKS_Docking_Nterm (3-30). E-value:
                     4.5e-09. Score: 27.7. Matches aSDomain:
                     nrpspksdomains_IE189_RS38605_PKS_Docking_Nterm.1"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (35-457). E-value:
                     1.2e-177. Score: 583.0. Matches aSDomain:
                     nrpspksdomains_IE189_RS38605_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (571-869). E-value: 1.5e-110.
                     Score: 361.1. Matches aSDomain:
                     nrpspksdomains_IE189_RS38605_PKS_AT.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1021: malonyl CoA-acyl carrier protein transacylase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174140.1"
                     /locus_tag="IE189_RS38605"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_189928746.1"
                     /sec_met_domain="mod_KS (E-value: 9.7e-228, bitscore:
                     743.1, seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 2e-90, bitscore:
                     289.9, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 5.1e-112, bitscore:
                     361.1, seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MADESKLRDYLKRTLAEARRSHQRVLELEAEKSEPVAVVGMACRL
                     PGGVAGPDDLWRLVSEGRDGMSGFPSDRGWDLEGVFDSAPGQAGTSYVPRGGFLHDAAE
                     FDAGFFGISPREALAMDPQQRLLLETSWEALEQAGIDPGALKGQEVAVFSGVYEQGYGF
                     STAELEGFIGTGAMTSVASGRVSYVLGLEGPAVTVDAACSSSLVAIHLAAQALRQGECS
                     LALAGGATVMATPGAFVAFSRHGGLAVDGRIKAFSDAADGTGWAEGVGVVVLERLSEAR
                     RRGHRILAVIRGSAVNQDGASNGLTAPNGPSQQQVIRKALANAGLSPADVDVVEGHGTG
                     TVLGDPIEAQALIATYGQDRPEDRPLWLGSLKSNIGHTQAAAGVAGVIKMVQALRHGIM
                     PPTLHVDAPSTKVDWTAGAVELLTEARAWPDTGRPRRAGVSSFGVSGTNAHLILEQAPQ
                     EQSVTPLESVPESMVDVGDGVVPLVVSAKSAGSLAGQAERLASFVESGETVSLTDVAGA
                     LVAQRAVLSERAVVVAGSRGEAVAGLEALARGDSHPGVVAGGLSSSVVAGRTVVVFPGQ
                     GSQWVGMGRELLDSSGVFAERVGECAAALERWVDWSLVDVLRGDAPVDVLERVDVVQPA
                     SFAVMVGLAAVWASVGVVPDAVVGHSQGEIAAACVAGALSLEDAARIVAVRSQVIAGDL
                     AGRGGMASVALSEAEALERLARWADRVEVAAVNGPSSVVIAGDAEALDEAVDVLEDQGV
                     RVRRIAVDYASHTRHVEAIEDVLATAFADIRAQAPLIPFYSTVTGGWVRESNVLDGGYW
                     YRNLRSQVRFGPAIADLLAGGHTVFVEASAHPVLVQPVNEIVDQAGTDAVVGGSLRRGE
                     GGPRRLLTSMAELFVRGVPVDWAGVLPAGAATTHVELPTYAFDHEHYWLRSAPVADAVA
                     LGQAGADHPLIGAVVAVPETGGVLCTSRLS"
     aSDomain        354..434
                     /aSDomain="PKS_Docking_Nterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38605_PKS_Docking_Nterm.
                     1"
                     /evalue="4.50E-09"
                     /label="IE189_RS38605_PKS_Docking_Nterm.1"
                     /locus_tag="IE189_RS38605"
                     /protein_end="30"
                     /protein_start="3"
                     /score="27.7"
                     /tool="antismash"
                     /translation="ESKLRDYLKRTLAEARRSHQRVLELEA"
     aSDomain        450..1715
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38605_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="1.20E-177"
                     /label="IE189_RS38605_PKS_KS.1"
                     /locus_tag="IE189_RS38605"
                     /protein_end="457"
                     /protein_start="35"
                     /score="583.0"
                     /tool="antismash"
                     /translation="VAVVGMACRLPGGVAGPDDLWRLVSEGRDGMSGFPSDRGWDLEGV
                     FDSAPGQAGTSYVPRGGFLHDAAEFDAGFFGISPREALAMDPQQRLLLETSWEALEQAG
                     IDPGALKGQEVAVFSGVYEQGYGFSTAELEGFIGTGAMTSVASGRVSYVLGLEGPAVTV
                     DAACSSSLVAIHLAAQALRQGECSLALAGGATVMATPGAFVAFSRHGGLAVDGRIKAFS
                     DAADGTGWAEGVGVVVLERLSEARRRGHRILAVIRGSAVNQDGASNGLTAPNGPSQQQV
                     IRKALANAGLSPADVDVVEGHGTGTVLGDPIEAQALIATYGQDRPEDRPLWLGSLKSNI
                     GHTQAAAGVAGVIKMVQALRHGIMPPTLHVDAPSTKVDWTAGAVELLTEARAWPDTGRP
                     RRAGVSSFGVSGTNAHLILEQAP"
     aSModule        450..2951
                     /domains="nrpspksdomains_IE189_RS38605_PKS_KS.1"
                     /domains="nrpspksdomains_IE189_RS38605_PKS_AT.1"
                     /incomplete
                     /locus_tags="IE189_RS38605"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       921..968
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38605_0001"
                     /evalue="2.60E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE189_RS38605"
                     /protein_end="208"
                     /protein_start="192"
                     /score="19.4"
                     /tool="antismash"
                     /translation="GPAVTVDAACSSSLVA"
     CDS_motif       1344..1388
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38605_0002"
                     /evalue="2.00E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="IE189_RS38605"
                     /protein_end="348"
                     /protein_start="333"
                     /score="10.7"
                     /tool="antismash"
                     /translation="VEGHGTGTVLGDPIE"
     CDS_motif       1449..1493
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38605_0003"
                     /evalue="9.80E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="IE189_RS38605"
                     /protein_end="383"
                     /protein_start="368"
                     /score="11.6"
                     /tool="antismash"
                     /translation="GSLKSNIGHTQAAAG"
     CDS_motif       1920..1946
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38605_0004"
                     /evalue="1.80E+01"
                     /label="PKSI-AT-mM_m5"
                     /locus_tag="IE189_RS38605"
                     /protein_end="534"
                     /protein_start="525"
                     /score="-0.3"
                     /tool="antismash"
                     /translation="SERAVVVAG"
     CDS_motif       2052..2108
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38605_0005"
                     /evalue="1.60E-09"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="IE189_RS38605"
                     /protein_end="588"
                     /protein_start="569"
                     /score="29.4"
                     /tool="antismash"
                     /translation="VVVFPGQGSQWVGMGRELL"
     aSDomain        2058..2951
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38605_PKS_AT.1"
                     /evalue="1.50E-110"
                     /label="IE189_RS38605_PKS_AT.1"
                     /locus_tag="IE189_RS38605"
                     /protein_end="869"
                     /protein_start="571"
                     /score="361.1"
                     /specificity="substrate consensus: mmal"
                     /specificity="PKS signature: Methylmalonyl-CoA"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="VFPGQGSQWVGMGRELLDSSGVFAERVGECAAALERWVDWSLVDV
                     LRGDAPVDVLERVDVVQPASFAVMVGLAAVWASVGVVPDAVVGHSQGEIAAACVAGALS
                     LEDAARIVAVRSQVIAGDLAGRGGMASVALSEAEALERLARWADRVEVAAVNGPSSVVI
                     AGDAEALDEAVDVLEDQGVRVRRIAVDYASHTRHVEAIEDVLATAFADIRAQAPLIPFY
                     STVTGGWVRESNVLDGGYWYRNLRSQVRFGPAIADLLAGGHTVFVEASAHPVLVQPVNE
                     IVDQAGTDAVVGGSLRR"
     CDS_motif       2220..2291
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38605_0006"
                     /evalue="7.50E-09"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="IE189_RS38605"
                     /protein_end="649"
                     /protein_start="625"
                     /score="27.9"
                     /tool="antismash"
                     /translation="LERVDVVQPASFAVMVGLAAVWAS"
     CDS_motif       2295..2405
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38605_0007"
                     /evalue="3.70E-20"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="IE189_RS38605"
                     /protein_end="687"
                     /protein_start="650"
                     /score="63.6"
                     /tool="antismash"
                     /translation="GVVPDAVVGHSQGEIAAACVAGALSLEDAARIVAVRS"
     CDS_motif       2394..2423
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38605_0008"
                     /evalue="1.40E+01"
                     /label="PKSI-AT-mM_m5"
                     /locus_tag="IE189_RS38605"
                     /protein_end="693"
                     /protein_start="683"
                     /score="-0.0"
                     /tool="antismash"
                     /translation="AVRSQVIAGD"
     CDS_motif       2514..2555
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38605_0009"
                     /evalue="4.00E-03"
                     /label="PKSI-AT-mM_m5"
                     /locus_tag="IE189_RS38605"
                     /protein_end="737"
                     /protein_start="723"
                     /score="9.6"
                     /tool="antismash"
                     /translation="AAVNGPSSVVIAGD"
     CDS_motif       2622..2654
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38605_0010"
                     /evalue="3.90E-04"
                     /label="PKSI-AT-mM_m6"
                     /locus_tag="IE189_RS38605"
                     /protein_end="770"
                     /protein_start="759"
                     /score="13.1"
                     /tool="antismash"
                     /translation="VDYASHTRHVE"
     CDS_motif       2763..2810
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38605_0011"
                     /evalue="3.40E-05"
                     /label="PKSI-AT-mM_m8"
                     /locus_tag="IE189_RS38605"
                     /protein_end="822"
                     /protein_start="806"
                     /score="15.9"
                     /tool="antismash"
                     /translation="LDGGYWYRNLRSQVRF"
ORIGIN
        1 accgtcgtct tcgactaccc caacccgctc tccctcgccc gccacctcca ccgcgaactc
       61 gggaccacca cgcaacccgt ggtcgcaacg catccgctgc tggctgaact gagcaggctc
      121 gatgccgcct tggccacgac gcccgccgac gattcggtcc gcgcccaagt gacagcccgg
      181 cttcagggtc ttctggcctc ctggtcgctg gcgaacggaa ccccgcccgc cgaagaggaa
      241 ctcgacttcg atgcggcctc ggacgacgaa ctcttcgacc tgatcgacac cgagttcggc
      301 aactagcaac cgccgcctgt cacagcctct aggagctgag cccgatggca gatgaaagca
      361 agcttcgcga ctacctcaag cgcaccctcg cggaggcacg ccgttcccat cagcgtgtgc
      421 tggagctcga ggccgagaag tcggagccgg ttgcggtggt gggtatggcg tgccgtctgc
      481 ccggtggtgt ggcgggtcct gacgatctgt ggcggctggt cagcgagggc cgggacggca
      541 tgtccggttt cccgtccgat cgtggctggg atctggaggg cgtgttcgac tccgccccgg
      601 gccaggccgg cacctcttac gtgccccggg gcgggtttct ccacgacgcg gcggagttcg
      661 acgcgggctt cttcgggatc tcgccgcgtg aggcgctggc gatggacccg cagcagcggt
      721 tgctgctgga gacgtcgtgg gaggcgctgg aacaggccgg gatcgacccg ggggcgctga
      781 agggccaaga ggtcgcggtg ttctccggcg tctacgaaca ggggtacggc ttcagcaccg
      841 cggaactgga agggttcatc ggcacgggcg cgatgacgag tgtcgcctcg ggccgggtgt
      901 cgtacgtgct cggtcttgag ggtccggcgg tgacggtgga cgcggcgtgt tcgtcctcgc
      961 tggtcgcgat ccatctggcg gcgcaggcgt tgcggcaggg ggagtgctcg ctggcgctgg
     1021 cgggcggcgc cacggtgatg gcgacaccgg gggcgttcgt ggcgttctcc cgccacggcg
     1081 gcctcgccgt cgacggacgg atcaaggcgt tctcggacgc cgcggacggt accggctggg
     1141 ccgagggcgt cggggtggtg gtcctggaac ggttgtcgga ggcccggcgg cgcgggcaca
     1201 ggatccttgc ggtgatccgt ggcagcgcgg tgaatcagga cggtgcgtcg aacggtctga
     1261 cggcgccgaa cggtccgtcg cagcagcagg tgatccgtaa ggcgctggcc aacgcgggtc
     1321 tttcgccggc ggatgtggat gtggtggagg gacacggcac gggcacggtc ctcggtgatc
     1381 cgatcgaggc gcaggcgctg atcgccacct atggacagga ccggcccgag gaccggccgc
     1441 tgtggctggg gtcgttgaag tcgaacatcg gtcataccca ggcggcggcg ggggtggccg
     1501 gtgtgatcaa gatggtgcag gcgctccggc acgggatcat gccgcccacg ctgcatgtcg
     1561 acgccccgtc gacgaaggtg gactggaccg cgggtgcggt cgagctgctg accgaggctc
     1621 gcgcctggcc tgacaccggc cggccgcgcc gcgcgggcgt gtcgtccttc ggcgtcagcg
     1681 ggacgaacgc gcacctgatc ctggagcagg ccccgcaaga gcagtccgtg acgccgctgg
     1741 agtctgtgcc tgagtcgatg gtcgacgtcg gcgatggtgt ggtgccgctg gtggtgtcgg
     1801 cgaagagtgc cggatccctg gcgggccagg ccgagcggtt ggcgtccttc gtggagtccg
     1861 gcgagacggt gtccctgacg gacgtggccg gggcgttggt cgctcagcgg gcggtgttgt
     1921 ccgagcgtgc ggtggtggtg gctggttcgc ggggtgaggc ggtggcgggt ctggaggcgc
     1981 tggcgcgggg cgactcccat cctggtgtgg tcgcaggcgg gttgtcgtcg tctgtggtcg
     2041 ctggtcggac ggttgtggtg tttcctgggc agggttcgca gtgggtgggg atggggcgtg
     2101 agctgctgga ttcgtcgggg gtgtttgcgg agcgggtggg ggagtgtgcg gcggcgttgg
     2161 agcggtgggt ggactggtcg ctcgtcgatg tgctgcgggg ggatgctccg gttgatgtgt
     2221 tggagcgggt ggatgtggtg cagccggcga gttttgcggt gatggtgggt ttggctgcgg
     2281 tgtgggcgtc ggtgggtgtg gtgccggatg cggtggtggg tcattcgcag ggtgagatcg
     2341 cggcggcgtg tgtggcgggt gcgttgtcgt tggaggacgc tgctcggatt gtggcggttc
     2401 gcagtcaggt catcgcgggt gatttggcgg gccggggtgg tatggcgtcg gtggcgctgt
     2461 ccgaggcgga ggcgcttgag cgtcttgcgc ggtgggctga tcgagtcgag gttgcggcgg
     2521 tcaacggtcc gtcctcggtg gtgatcgcgg gtgatgccga ggcgctcgac gaggccgtcg
     2581 acgtgctgga agaccaaggc gtacgcgtgc gccgcatcgc cgtcgactac gcctcgcaca
     2641 cccggcatgt ggaggcgatc gaggatgtac tggcaaccgc cttcgccgac atccgtgcgc
     2701 aggccccgct gattcccttc tactcgaccg tgacgggtgg gtgggtgcgg gagtcgaatg
     2761 tcctcgacgg cggttactgg taccggaacc tgcggagcca ggtccgtttc ggtcccgcca
     2821 ttgccgacct cctggctggt ggtcacaccg tcttcgtgga ggccagtgcc catcccgtcc
     2881 tggtccagcc ggtcaacgag atcgtcgacc aggccggcac agacgccgtg gtcggtggtt
     2941 cactgcgccg cggggagggc ggcccgcgcc gactgctgac ctcgatggcc gagctgttcg
     3001 tccggggcgt ccccgtcgac tgggcgggtg tcctcccggc cggtgccgcc accacccacg
     3061 tggaactccc cacgtatgcc ttcgatcacg agcactactg gctgcgttcc gctccggttg
     3121 ccgatgctgt tgcgctggga caggcgggtg ctgatcatcc gctgatcggt gcggtggtgg
     3181 cggtgccgga gaccggtggt gtcctgtgca cgtctcggtt gtct
//
