LOCUS       NZ_BMSI01000038         4332 bp    DNA     linear   CON 02-AUG-2024
DEFINITION  Streptomyces asoensis strain JCM 4185 sequence38, whole genome
            shotgun sequence.
ACCESSION   NZ_BMSI01000038
VERSION     NZ_BMSI01000038.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces asoensis
  ORGANISM  Streptomyces asoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649075.1-RS_2024_08_02
            Annotation Date                   :: 08/02/2024 04:45:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,813
            CDSs (total)                      :: 7,736
            Genes (coding)                    :: 7,563
            CDSs (with protein)               :: 7,563
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 53 of 173
            Pseudo Genes (incomplete)         :: 140 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 28 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:21:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 4332
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSI01000038.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     protocluster    1..4332
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[<0:965](-)"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(<1..965)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="AfsA"
                     /neighbourhood="5000"
                     /product="butyrolactone"
                     /protocluster_number="1"
     gene            complement(<1..965)
                     /locus_tag="IE189_RS38560"
     CDS             complement(<1..965)
                     /GO_function="GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     butyrolactone: AfsA"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008406753.1"
                     /locus_tag="IE189_RS38560"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="ScbA/BarX family gamma-butyrolactone biosynthesis
                     protein"
                     /protein_id="WP_189928740.1"
                     /sec_met_domain="AfsA (E-value: 1.8e-27, bitscore: 83.1,
                     seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSVSTFHVERQATATRTPGKDTTAPAYLTVPHFPSLTSTVPRELV
                     HRASIAEVMLTGWQRQDDTHFTVSAQLPRRHGFFETVQGCHDPLLIAETVRQTGLLLAH
                     TEFGVPFGHQFLMWNLAVDLRHEHLLVGATPASLDIHVTCTDIKRRGTQLSGLHLTAAI
                     HRDGTPAATGTAAFTCTSPAVYRRLRAHRLNNTTPAPLPLTSPTAPQTVGRHSPTDVVL
                     SPHNQPDTWQLRIDTRHPVLFDHPLDHVPGMALIEAARQAATAYLGHPCLPTTLHSEFT
                     HYAELHTPTIIKTHPQPPHPHGHTRLLITGHQNNTPIFTTT"
     source          1..4332
                     /culture_collection="JCM:4185"
                     /db_xref="taxon:249586"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces asoensis"
                     /strain="JCM 4185"
                     /submitter_seqid="sequence38"
                     /type_material="type strain of Streptomyces cacaoi subsp.
                     asoensis"
     region          1..4332
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="butyrolactone"
                     /region_number="1"
                     /rules="AfsA"
                     /tool="antismash"
     cand_cluster    1..4332
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="AfsA"
                     /kind="single"
                     /product="butyrolactone"
                     /protoclusters="1"
                     /tool="antismash"
     misc_feature    complement(1211..1224)
                     /note="TFBS match to LexA_variant_2, Repressor of DNA
                     damage response, confidence: medium, score: 20.16"
                     /tool="antismash"
     misc_feature    1211..1224
                     /note="TFBS match to PsrA, Oleic acid responsive regulator,
                     confidence: medium, score: 21.39"
                     /tool="antismash"
     misc_feature    1217..1226
                     /note="TFBS match to ToxT, Regulator of MSHA biosynthesis,
                     confidence: weak, score: 14.85"
                     /tool="antismash"
     gene            3683..3874
                     /locus_tag="IE189_RS38565"
                     /old_locus_tag="GCM10010496_78180"
     CDS             3683..3874
                     /GO_function="GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA]"
                     /GO_function="GO:0004658 - propionyl-CoA carboxylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569602.1"
                     /locus_tag="IE189_RS38565"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_78180"
                     /product="acyl-CoA carboxylase epsilon subunit"
                     /protein_id="WP_189928741.1"
                     /transl_table=11
                     /translation="MGRTQSLIRVERGRPDERELAAVVAVLLALRAGAQPECAGEPAAA
                     EMSWWRMPQVYAAPDGWR"
     misc_feature    complement(3935..3948)
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.49"
                     /tool="antismash"
     misc_feature    3936..3949
                     /note="TFBS match to PerR, Peroxide regulator, confidence:
                     weak, score: 13.86"
                     /tool="antismash"
     gene            4005..>4332
                     /locus_tag="IE189_RS38570"
     CDS             4005..>4332
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019983029.1"
                     /locus_tag="IE189_RS38570"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="WP_308426832.1"
                     /transl_table=11
                     /translation="MTTAPAPAGRALEPTDIRGRVAELHSIREQALAGPGGRATAAQHA
                     KGKLTARERIELLLDPGSFQEVEQLRRHRATGFGMEVKKPYTDGVVTGWGTVEGRTVFV
                     YAHDF"
ORIGIN
        1 gtggtggtga agatgggggt gttgttttgg tggccggtga tgagcaggcg ggtgtggccg
       61 tgggggtggg ggggttgggg gtgggttttg atgatggtgg gggtgtggag ttcggcgtag
      121 tgggtgaatt cgctgtgcag ggtggtgggg aggcaggggt ggccgaggta ggcggtggcg
      181 gcctgtcggg cggcttcgat gagggccatg ccggggacgt ggtcgagggg gtggtcgaag
      241 aggacggggt ggcgggtgtc gatgcgtagt tgccaggtgt cgggctggtt gtggggggac
      301 aggacgacgt cggtggggga gtggcggccg acggtctggg gtgcggtggg gctggtcagg
      361 ggcaggggtg cgggggtggt gttgttgagg cggtgggcgc gcaggcgtcg gtagacggct
      421 ggggaggtgc aggtgaaggc ggcggtgccg gtcgcggcgg gggtgccgtc gcggtggatg
      481 gcggcggtga ggtgcagtcc ggacagctgg gtgccgcggc gtttgatgtc ggtgcaggtg
      541 acgtggatgt ccagggaggc gggggtggcg ccgacgagga ggtgttcgtg tcggaggtcg
      601 acggcgaggt tccacatgag gaactggtgg ccgaagggta cgccgaactc ggtgtgggcg
      661 agcaggagtc cggtctggcg gacggtttcc gcgatgagca gggggtcgtg gcagccctgg
      721 acggtttcga agaagccgtg ccggcggggg agttgggcgc tgacggtgaa gtgggtgtcg
      781 tcctggcgtt gccagccggt cagcatcacc tcggcgatgc tggcgcggtg gacgagttct
      841 ctggggacgg tgctggtcag ggacgggaag tgcgggacgg tgaggtaggc cggggccgtg
      901 gtgtccttgc ccggagtgcg ggtggcggtg gcctgtcgtt cgacgtggaa agtgctcaca
      961 gacatgtctc tccccctgag ccgcatgaag cgtgacgtcg atggaacggg ccctgtgggg
     1021 cccctcaggc ctgcgaagat acataccacc cggtttaatt tctagcggta cccgtcattc
     1081 cggccgcgcg gtggagcgaa cggcttgttc cccgtcgcgg cccccgtccg cctcccggcc
     1141 cgccggacag gggctgaact ccggctttac cgcgggacga gggctgctcg agaggtgctc
     1201 gcacgccggt gaaacgtatg ttttgagcca tgtgggcgcg ggtggtgggg gtgcgggtga
     1261 tcggatgccc gtagggaacg ggctgtggcg tgtctgctgc gggggcccgg acctgcggcg
     1321 ggtgcgggcg gggccgctgc ggggagggcc cgtgtcccgg gggaaccgca tgcgcggtct
     1381 gttgtgtggg ccgggggcgg gccggggtgg gcctggggcg gctcgtgggc ggcctggtgc
     1441 tcgcccgtcc gtcagccgtg gcccaggtcg gcccggctgt cggcgtgctc ggcctttgtg
     1501 gagggcgcgg ggcgggcggg cgcttgcggt cttccgggtc ggcggggcgg gtgggtgttc
     1561 ccgggcctcc ggggcgctcc ggggcgggcg ggcgctcgcg gtctcctggc tcctggcccg
     1621 gcgcagcacc gtcggtcccg gtccggtccc gggtcctggc cgagggcgtc tgcggttgtc
     1681 ggggtcccgg gggcgctccc gggtcctgtc gccgggtctg gcccgtctgc ggatcttgtt
     1741 ttcgggttct gttgccgagg ccggcccgtg gcggggtccg gcctcgggtc tttcggcgtg
     1801 gggtctgtct cttgggcggg tgccgggtgg gggtacctgt gaggccgggg ggctggtggg
     1861 tggtcgtgag ggggtcgttg gtgcggccgg gccgtgccag ggccttgtgc ccgctcgttg
     1921 ccgggggcag cggctggttg cgccacgggt actgcgccgt gtccgtcccg cgcgggtgca
     1981 gttgcacgcg cgcgggacgg ggaagggggg ggtagagggc tggggcgact cgctgaagcg
     2041 gcggcggact tcatcggggc gtcgggatcc ctgcgggggc gggtgatggg ggcccgcgtg
     2101 gcccgtgccc gtccggtgtc cgggggccgg gccgtgttcg ccggctttct gtgtcagtcc
     2161 ttgccgtctg tctcctgccg tcggtctggg gtcggctgtc cgtctgtgat cggttcagcc
     2221 tgtgatggcg tgcgggatcg agccggccgg gtgtgccggg ccggccggcg agtgctccga
     2281 gcgggccggt cgggtgggcg ccggcggtgg ccagtgcacc tgcggaggac ggcagccggg
     2341 tgatcgggcc gagccggccc gccgcggcct gccggcccgc cacggtctgc cggtccggct
     2401 cgggccgggc gacttcctcc tccagcgagt ccggttcggc ccgcgcctcg ggtttgatcg
     2461 ccgctggcag tgccgtgcgc tcgagcgacg tccctcaggt cccggaccag ccgcacgcct
     2521 cctgcccgcc gtgaagacgg ccggctggcc gccggtgaac gctctggttc ccggtgacgt
     2581 tgccgaccgt gccgccctgc tggttgccga tgtcggggaa cctggccgct cctcttcttg
     2641 cttgctcacg gctgccgccg ggccgcctgc cgttcggcgt cctcgaagcg ccggcgccgg
     2701 gggtgccgtc acgatgtgcc cagcggtccc ggcggccgtc aggaccgtcc cgggggccgg
     2761 gatcgccggc ccggccgccc cggccggcac ccgcccgctg caagccggtg ccctccccgc
     2821 cgcgcacgag acggtcgtgt cccgtatccg tatcccccga agccggcggg cgcggccgcg
     2881 gacttgagcc ctcctcgccc tgtgtgggca ctccccgccc gaggcctggg cggggcacgc
     2941 actccccgcc cgctgggtgc gctgcctgcc gcccccggcg tacttcaccg ccccgcacct
     3001 tgacgctccg gccccggcgt cctgccggac tttgcccctc ccggcgtccc cctgccccct
     3061 gcccccggcg caccgcccca gggcctccac gtccccgcgt gctgccgcac ccccgggcgt
     3121 ccgccgcctc agggcgtttc acggccccgg cattctgccg ggccccgccc ctcccggccg
     3181 ccgctgtacc ccccctcccg gcgttccgcc gccccttagg gccgttcacg gccccgccgt
     3241 cccgtaaccc cccccgcccg ggcaccggcc cccgctgcac cgcccgcctg ccgcccccgg
     3301 cggtcctgcc ccggccccgg agggcagccc gcctgcgcac tccccccgcg gtcctgcccc
     3361 ggccccggag ggcagcccgc gccgggcagc ccgcgccggg cagccccccg gggtgcgggt
     3421 ccgtgcagcc ggggtgcagg cccgtacagc cctcagggtg cagccctccg gggtgcgggc
     3481 ccgtgcagcc ggggtgcggg cccgtacagc ccccgaaagc gggcctcccg gtccccggct
     3541 cttcgggggt gcggctcgtg aagtggcagg tcgtcggggt gggtggggtg ggggtcttgt
     3601 gggtctcgag tgatctcaaa gcggttcttg agtgtcctgg ccgacggtgt cttgcatggt
     3661 ccgagaagag ggggacggtg cgatggggag aacgcaatcc ctgatcaggg tggagcgggg
     3721 gcggcccgac gagcgggagc tggccgcggt ggtggcggtg ctgctcgcgc tgcgggcggg
     3781 cgcgcagccc gagtgtgccg gggaaccggc ggcggccgaa atgagctggt ggcgtatgcc
     3841 tcaggtctac gcggcccccg acggctggcg ctgacacggc ggggatgcgt gctggtcccc
     3901 gcccgaccga aaccaaaacc gcattgacgg tttgttatga tgcttcttac agacccgggg
     3961 ccagacctcc tcggccttgt cccaggaagg cacctgacat ggcgatgacg actgccccgg
     4021 ccccggccgg acgcgcactg gagcccaccg acatccgcgg ccgtgtggcg gaactgcaca
     4081 gcatccgcga gcaggcactg gccggccccg gcgggagggc caccgccgcg cagcacgcca
     4141 agggcaagct gaccgcccgc gagcgcatcg aactgctcct ggatcccggc tccttccagg
     4201 aggtcgagca gctgcgccgg caccgcgcga ccgggttcgg catggaggtc aagaagccct
     4261 acaccgacgg ggtcgtcacc ggctggggca cggtcgaggg ccgcacggtc ttcgtctacg
     4321 cccacgactt cc
//
