LOCUS       NZ_BMSI01000035         7692 bp    DNA     linear   CON 02-AUG-2024
DEFINITION  Streptomyces asoensis strain JCM 4185 sequence35, whole genome
            shotgun sequence.
ACCESSION   NZ_BMSI01000035
VERSION     NZ_BMSI01000035.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces asoensis
  ORGANISM  Streptomyces asoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 7692)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649075.1-RS_2024_08_02
            Annotation Date                   :: 08/02/2024 04:45:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,813
            CDSs (total)                      :: 7,736
            Genes (coding)                    :: 7,563
            CDSs (with protein)               :: 7,563
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 53 of 173
            Pseudo Genes (incomplete)         :: 140 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 28 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:21:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 7692
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSI01000035.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..2686
                     /locus_tag="IE189_RS38505"
                     /old_locus_tag="GCM10010496_78100"
     source          1..7692
                     /culture_collection="JCM:4185"
                     /db_xref="taxon:249586"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces asoensis"
                     /strain="JCM 4185"
                     /submitter_seqid="sequence35"
                     /type_material="type strain of Streptomyces cacaoi subsp.
                     asoensis"
     region          1..7692
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..7692
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..7692
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[3003:>7692](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      3004..>7692
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS             <3..2686
                     /NRPS_PKS="Domain: PKS_KR (424-607). E-value: 6.2e-42.
                     Score: 135.5. Matches aSDomain:
                     nrpspksdomains_IE189_RS38505_PKS_KR.1"
                     /NRPS_PKS="Domain: PKS_PP (701-787). E-value: 7.8e-28.
                     Score: 88.7. Matches aSDomain:
                     nrpspksdomains_IE189_RS38505_PKS_PP.1"
                     /NRPS_PKS="Domain: PKS_Docking_Cterm (794-868). E-value:
                     2e-08. Score: 26.9. Matches aSDomain:
                     nrpspksdomains_IE189_RS38505_PKS_Docking_Cterm.1"
                     /codon_start=2
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PP-binding"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1047: phosphopantetheine-binding domain-containing
                     protein"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /locus_tag="IE189_RS38505"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_78100"
                     /product="KR domain-containing protein"
                     /protein_id="WP_189928730.1"
                     /sec_met_domain="PKS_AT (E-value: 2e-19, bitscore: 57.8,
                     seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 1.6e-40, bitscore:
                     126.7, seeds: 230, tool: rule-based-clusters)"
                     /sec_met_domain="PP-binding (E-value: 1.5e-13, bitscore:
                     38.9, seeds: 164, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="TVIGEWVKEADVLDGGYWYRNLRSQVRFGPAVADLLAGGHTVFVE
                     ASAHPVLVQPVNEIVDEADDTVRLRAVVGGSLRRGEGGLHRLFTSMAELFVRGVPVDWA
                     AMLPAGASPVELPTYAFDRRHYWLQEADDADGSGSPGEDIDADFWSAVERSDADSLAGL
                     LAPDSAGLRDALRTVVPVLADWRGRRQQRSSAERLRYAVTWRPLDREVTGVPAGRWLAV
                     LPPGCPTGDATGVPADSTAGSQVLELLAALAAQGLDIVPLEIATSAFTRTGFTEQLRGI
                     RAAYEPAGVLSLFALDGTQDATVTAARTLALIQALGDAGVDGPLWCLTRGVVNTGVQDT
                     VTDPGEAALWGLGRAVALEHPDRWGGLVDLPATVNSRTIQYLLGALNGAAGDDQLAVRR
                     SGVYSRRLTRKPAPEAPADGGWQPHGTVLVTGGAEALGAHASLWLARSGVRRLIITTTA
                     QAPADAVTTLLDELAAAEVATTVVSCADADRDTLARLIAETPREQPLTAVVHAADIAWT
                     GNIADTGLADLTEVFAAKVDTAVWLDAMFTETGTGGAPLDAFVVFSSIAGIWGGGGQGV
                     SGAANAVLDALVERRRGRGLAATSIAWGALDEIGPAMDEATLAQLRRRGVLPMTRQIAT
                     TAFEQAVQAREKAVTVVDMDWEAFIPAFTSGRVSPLFADLPEAAAALRASQPDAEDIGT
                     TSSLVDSLRNVPEAEQNRLLLRLVCGQASTVLGHSSTDSIGPLQSFQEVGFDSLAAVNL
                     RNSLHVATGLRLPATLIFDYPTPDALVGFLRSELLTEADDDLDAREDDLRRVLAQVPLA
                     RFREAGLLDTLLGLVGPEDGSAPGVTTPVPDPVVPVAADDADLIDVMDVADLVKRALGD
                     GTDFLGGDRELPDQGMPREGGPA"
     CDS_motif       41..88
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38505_0001"
                     /evalue="3.10E-05"
                     /label="PKSI-AT-mM_m8"
                     /locus_tag="IE189_RS38505"
                     /protein_end="28"
                     /protein_start="12"
                     /score="16.1"
                     /tool="antismash"
                     /translation="LDGGYWYRNLRSQVRF"
     aSDomain        1277..1825
                     /ASF="KR domain putatively catalyzing L-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38505_PKS_KR.1"
                     /evalue="6.20E-42"
                     /label="IE189_RS38505_PKS_KR.1"
                     /locus_tag="IE189_RS38505"
                     /protein_end="607"
                     /protein_start="424"
                     /score="135.5"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="GTVLVTGGAEALGAHASLWLARSGVRRLIITTTAQAPADAVTTLL
                     DELAAAEVATTVVSCADADRDTLARLIAETPREQPLTAVVHAADIAWTGNIADTGLADL
                     TEVFAAKVDTAVWLDAMFTETGTGGAPLDAFVVFSSIAGIWGGGGQGVSGAANAVLDAL
                     VERRRGRGLAATSIAWGALD"
     aSModule        1277..2365
                     /domains="nrpspksdomains_IE189_RS38505_PKS_KR.1"
                     /domains="nrpspksdomains_IE189_RS38505_PKS_PP.1"
                     /incomplete
                     /locus_tags="IE189_RS38505"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       1280..1369
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38505_0002"
                     /evalue="1.80E-06"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE189_RS38505"
                     /protein_end="455"
                     /protein_start="425"
                     /score="20.6"
                     /tool="antismash"
                     /translation="TVLVTGGAEALGAHASLWLARSGVRRLIIT"
     CDS_motif       1670..1780
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38505_0003"
                     /evalue="4.50E-14"
                     /label="PKSI-KR_m4"
                     /locus_tag="IE189_RS38505"
                     /protein_end="592"
                     /protein_start="555"
                     /score="44.9"
                     /tool="antismash"
                     /translation="LDAFVVFSSIAGIWGGGGQGVSGAANAVLDALVERRR"
     aSDomain        2108..2365
                     /aSDomain="PKS_PP"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38505_PKS_PP.1"
                     /evalue="7.80E-28"
                     /label="IE189_RS38505_PKS_PP.1"
                     /locus_tag="IE189_RS38505"
                     /protein_end="787"
                     /protein_start="701"
                     /score="88.7"
                     /tool="antismash"
                     /translation="LRNVPEAEQNRLLLRLVCGQASTVLGHSSTDSIGPLQSFQEVGFD
                     SLAAVNLRNSLHVATGLRLPATLIFDYPTPDALVGFLRSEL"
     aSDomain        2387..2608
                     /aSDomain="PKS_Docking_Cterm"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38505_PKS_Docking_Cterm.
                     1"
                     /evalue="2.00E-08"
                     /label="IE189_RS38505_PKS_Docking_Cterm.1"
                     /locus_tag="IE189_RS38505"
                     /protein_end="868"
                     /protein_start="794"
                     /score="26.9"
                     /tool="antismash"
                     /translation="LDAREDDLRRVLAQVPLARFREAGLLDTLLGLVGPEDGSAPGVTT
                     PVPDPVVPVAADDADLIDVMDVADLVKRA"
     gene            2783..2971
                     /locus_tag="IE189_RS38510"
                     /old_locus_tag="GCM10010496_78110"
     CDS             2783..2971
                     /codon_start=1
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /locus_tag="IE189_RS38510"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /old_locus_tag="GCM10010496_78110"
                     /product="hypothetical protein"
                     /protein_id="WP_189928731.1"
                     /transl_table=11
                     /translation="MPLGPVGPEDGSAPGVTTPVPDPVMPVAADDADLIDVMDVADLVQ
                     RVPGDDTDRSTDLPTFG"
     gene            3004..>7692
                     /locus_tag="IE189_RS38515"
     CDS             3004..>7692
                     /EC_number="6.4.-.-"
                     /NRPS_PKS="Domain: PKS_KS(Modular-KS) (36-462). E-value:
                     3.2e-171. Score: 561.8. Matches aSDomain:
                     nrpspksdomains_IE189_RS38515_PKS_KS.1"
                     /NRPS_PKS="Domain: PKS_AT (577-875). E-value: 8.4e-109.
                     Score: 355.4. Matches aSDomain:
                     nrpspksdomains_IE189_RS38515_PKS_AT.1"
                     /NRPS_PKS="Domain: PKS_DH (944-1115). E-value: 1.3e-64.
                     Score: 209.4. Matches aSDomain:
                     nrpspksdomains_IE189_RS38515_PKS_DH.1"
                     /NRPS_PKS="Domain: PKS_KR (1430-1555). E-value: 2.9e-35.
                     Score: 113.8. Matches aSDomain:
                     nrpspksdomains_IE189_RS38515_PKS_KR.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     mod_KS"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) adh_short"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_121825819.1"
                     /locus_tag="IE189_RS38515"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_189928732.1"
                     /sec_met_domain="mod_KS (E-value: 1e-221, bitscore: 723.8,
                     seeds: 217, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 2.3e-87, bitscore:
                     280.4, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 4.2e-110, bitscore:
                     355.4, seeds: 1682, tool: rule-based-clusters)"
                     /sec_met_domain="adh_short (E-value: 3.5e-44, bitscore:
                     138.6, seeds: 230, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPDEQIVNALRASLKENTRLQQENQRLSESSAEPIAIVSMACR
                     YAGGIRTPEDLWRVVTDGTDVYTTFPEDRGWDLEGLYHPDPDNPGTTYVREGAFLHDAG
                     QFDAAFFGISPREALAMDPQQRQLLEVSWEALERAGIDPHSVRGADIGVFAGMVHQDYA
                     PDLSGLEGFLSLERALGTAGGVASGRVAYTLGLEGPAVTVDTMCSSSMVAIHLAAQALR
                     RGECSMALAGGATVMATPGGFVGFARQRGLAFDGRCKSYAACADGSSWAEGAGVILLER
                     LSEARRNGHRVLAVIRGSALNQDGASNGLTAPNGPAQQRVIRKALESADLTTADIDMVE
                     GHGTGTVLGDPIEAQALIATYGQDRSEDRPLWLGSVKSVIGHTQAASGVAAVINMVQAL
                     RHGVMPATRHVDAPTSQVDWTAGSVELLTEARPWPEVGRPRRAGASSFGASGTNAHLIL
                     EQAPEEPAAETAAGPSPAPAVDGVVPLVLSAATAASLTGQAERLLSFVETAGVAGTAAL
                     ADVATALATGRATLAERAVVVADSPEEALAGLGALARGEAARAVAVGSASTPGGAGKVV
                     LVFPGQGSQWTGMGRELLDSSPVFAERIAECAAALERWVDWSLVDVLRGDAPAELLKRV
                     DVLQPASFAVMIGLAAVWSSVGVVPDAVVGHSQGEIAAACVSGALSLENAARIVAVRSQ
                     VIAASLAGRGGMASVALSEEEAVERLERWAGRIEVAAVNGPVSVVIAGDAEALDEAVDV
                     LEDQGVRVRRIAVDYASHTRHVEAVQETLSEAFADIRSQAPAVPFLSTVTGEWISEAGV
                     LDGGYWYRNLRRQVRFGPAIADLLAGGHTVFVESSAHPVLVQPISEIVVRAEADAVVTG
                     SLRREDGGPRRLFTSMAELFVRGVHVDWNGVLAAGTDAHRVDLPTYAFDHRHYWIQLTG
                     TADDAASLGLTGTDHPLLGAVVPLPQSDGLVLTSRLSLRTHPWLADHAIAGAVVVPGTI
                     YVDLAVRAGDEFGCGVLDELVIESPLVLPEQGGVRIQVAVSGAGPNGSRTVDVYSLREE
                     TSDGAGQGEWTRHATGLLSQAARADATTPAGDFAVWPPAGAQPVAVDGFYSDLAARGYA
                     YGPAFQGLRGVWRRGDELFAEAVLPPEQRENADRFGIHPALLDAALHTNAFANPDDDRR
                     VLPFAWNGLVLHAQGASALRVRVAPSGPDALSFQAADETGGLVLTMDSLVSLPVAAEQL
                     GSGAAGRDSRDSLFRVEWTLLPAPEGAAAPASWASLAGVDDVTALAARETLPPVAVLTA
                     TTGDGEDPLGLTTRVVAVVQAWLAAAPALDAVPLVVVTRGAVPAGGDADVTDPAGAAVW
                     GLIRAAQAENPDRIVLVDLDPAGSAGAGPVLGPVLATGEPLVAVRGTALSAPRLVRAAA
                     VETERAADADAGAETRTRTESAYAFAPGGTVLVTGGTGSLGAMVARHLVAGHGVRRLLL
                     VSRRGSDAEGSAELVAELGESGAVVTVCACDVADRDAVAAVLAAVPVEHPLTGVVHLAG
                     VLDDGVIGALTPERIEGVFAPKVTAVRHLDELTRELAPQLASF"
     misc_feature    complement(3017..3032)
                     /note="TFBS match to RutR, Regulator of the pyrimidine and
                     purine metabolism, confidence: weak, score: 19.73"
                     /tool="antismash"
     aSDomain        3112..4389
                     /ASF="found active site cysteine: True, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38515_PKS_KS.1"
                     /domain_subtypes="Modular-KS"
                     /evalue="3.20E-171"
                     /label="IE189_RS38515_PKS_KS.1"
                     /locus_tag="IE189_RS38515"
                     /protein_end="462"
                     /protein_start="36"
                     /score="561.8"
                     /tool="antismash"
                     /translation="IAIVSMACRYAGGIRTPEDLWRVVTDGTDVYTTFPEDRGWDLEGL
                     YHPDPDNPGTTYVREGAFLHDAGQFDAAFFGISPREALAMDPQQRQLLEVSWEALERAG
                     IDPHSVRGADIGVFAGMVHQDYAPDLSGLEGFLSLERALGTAGGVASGRVAYTLGLEGP
                     AVTVDTMCSSSMVAIHLAAQALRRGECSMALAGGATVMATPGGFVGFARQRGLAFDGRC
                     KSYAACADGSSWAEGAGVILLERLSEARRNGHRVLAVIRGSALNQDGASNGLTAPNGPA
                     QQRVIRKALESADLTTADIDMVEGHGTGTVLGDPIEAQALIATYGQDRSEDRPLWLGSV
                     KSVIGHTQAASGVAAVINMVQALRHGVMPATRHVDAPTSQVDWTAGSVELLTEARPWPE
                     VGRPRRAGASSFGASGTNAHLILEQAP"
     aSModule        3112..7668
                     /domains="nrpspksdomains_IE189_RS38515_PKS_KS.1"
                     /domains="nrpspksdomains_IE189_RS38515_PKS_AT.1"
                     /domains="nrpspksdomains_IE189_RS38515_PKS_DH.1"
                     /domains="nrpspksdomains_IE189_RS38515_PKS_KR.1"
                     /incomplete
                     /locus_tags="IE189_RS38515"
                     /tool="antismash"
                     /type="pks"
     CDS_motif       3595..3642
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38515_0001"
                     /evalue="4.70E-06"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE189_RS38515"
                     /protein_end="213"
                     /protein_start="197"
                     /score="18.6"
                     /tool="antismash"
                     /translation="GPAVTVDTMCSSSMVA"
     CDS_motif       4018..4062
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38515_0002"
                     /evalue="3.20E-03"
                     /label="PKSI-KS_m5"
                     /locus_tag="IE189_RS38515"
                     /protein_end="353"
                     /protein_start="338"
                     /score="10.2"
                     /tool="antismash"
                     /translation="VEGHGTGTVLGDPIE"
     CDS_motif       4123..4167
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38515_0003"
                     /evalue="5.00E-03"
                     /label="PKSI-KS_m6"
                     /locus_tag="IE189_RS38515"
                     /protein_end="388"
                     /protein_start="373"
                     /score="9.7"
                     /tool="antismash"
                     /translation="GSVKSVIGHTQAASG"
     CDS_motif       4729..4785
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38515_0004"
                     /evalue="2.20E-09"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="IE189_RS38515"
                     /protein_end="594"
                     /protein_start="575"
                     /score="28.9"
                     /tool="antismash"
                     /translation="VLVFPGQGSQWTGMGRELL"
     aSDomain        4735..5628
                     /ASF="(Methyl)Malonyl-CoA specificity inconclusive"
                     /ASF="found active site serine: True, scaffold matched
                     GHGE: True"
                     /aSDomain="PKS_AT"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38515_PKS_AT.1"
                     /evalue="8.40E-109"
                     /label="IE189_RS38515_PKS_AT.1"
                     /locus_tag="IE189_RS38515"
                     /protein_end="875"
                     /protein_start="577"
                     /score="355.4"
                     /specificity="substrate consensus: mmal"
                     /specificity="PKS signature: Methylmalonyl-CoA"
                     /specificity="Minowa: mmal"
                     /tool="antismash"
                     /translation="VFPGQGSQWTGMGRELLDSSPVFAERIAECAAALERWVDWSLVDV
                     LRGDAPAELLKRVDVLQPASFAVMIGLAAVWSSVGVVPDAVVGHSQGEIAAACVSGALS
                     LENAARIVAVRSQVIAASLAGRGGMASVALSEEEAVERLERWAGRIEVAAVNGPVSVVI
                     AGDAEALDEAVDVLEDQGVRVRRIAVDYASHTRHVEAVQETLSEAFADIRSQAPAVPFL
                     STVTGEWISEAGVLDGGYWYRNLRRQVRFGPAIADLLAGGHTVFVESSAHPVLVQPISE
                     IVVRAEADAVVTGSLRR"
     CDS_motif       4897..4968
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38515_0005"
                     /evalue="3.40E-07"
                     /label="PKSI-AT-mM_m2"
                     /locus_tag="IE189_RS38515"
                     /protein_end="655"
                     /protein_start="631"
                     /score="22.9"
                     /tool="antismash"
                     /translation="LKRVDVLQPASFAVMIGLAAVWSS"
     CDS_motif       4972..5082
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38515_0006"
                     /evalue="2.90E-18"
                     /label="PKSI-AT-mM_m3"
                     /locus_tag="IE189_RS38515"
                     /protein_end="693"
                     /protein_start="656"
                     /score="57.7"
                     /tool="antismash"
                     /translation="GVVPDAVVGHSQGEIAAACVSGALSLENAARIVAVRS"
     CDS_motif       5191..5229
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38515_0007"
                     /evalue="3.10E-03"
                     /label="PKSI-AT-M_m5"
                     /locus_tag="IE189_RS38515"
                     /protein_end="742"
                     /protein_start="729"
                     /score="10.9"
                     /tool="antismash"
                     /translation="AAVNGPVSVVIAG"
     CDS_motif       5299..5331
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38515_0008"
                     /evalue="7.00E-04"
                     /label="PKSI-AT-mM_m6"
                     /locus_tag="IE189_RS38515"
                     /protein_end="776"
                     /protein_start="765"
                     /score="12.4"
                     /tool="antismash"
                     /translation="VDYASHTRHVE"
     CDS_motif       5440..5487
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38515_0009"
                     /evalue="6.20E-05"
                     /label="PKSI-AT-mM_m8"
                     /locus_tag="IE189_RS38515"
                     /protein_end="828"
                     /protein_start="812"
                     /score="15.2"
                     /tool="antismash"
                     /translation="LDGGYWYRNLRRQVRF"
     aSDomain        5836..6348
                     /ASF="catalytic triad H,G,P found: False"
                     /aSDomain="PKS_DH"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38515_PKS_DH.1"
                     /evalue="1.30E-64"
                     /label="IE189_RS38515_PKS_DH.1"
                     /locus_tag="IE189_RS38515"
                     /protein_end="1115"
                     /protein_start="944"
                     /score="209.4"
                     /tool="antismash"
                     /translation="HPLLGAVVPLPQSDGLVLTSRLSLRTHPWLADHAIAGAVVVPGTI
                     YVDLAVRAGDEFGCGVLDELVIESPLVLPEQGGVRIQVAVSGAGPNGSRTVDVYSLREE
                     TSDGAGQGEWTRHATGLLSQAARADATTPAGDFAVWPPAGAQPVAVDGFYSDLAARGYA
                     YGPAFQGL"
     CDS_motif       5920..5994
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38515_0010"
                     /evalue="1.50E-07"
                     /label="PKSI-DH_m2"
                     /locus_tag="IE189_RS38515"
                     /protein_end="997"
                     /protein_start="972"
                     /score="23.9"
                     /tool="antismash"
                     /translation="WLADHAIAGAVVVPGTIYVDLAVRA"
     aSDomain        7294..7668
                     /ASF="KR domain putatively catalyzing D-configuration
                     product formation"
                     /ASF="catalytic triad S,Y,N inconclusive"
                     /aSDomain="PKS_KR"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38515_PKS_KR.1"
                     /evalue="2.90E-35"
                     /label="IE189_RS38515_PKS_KR.1"
                     /locus_tag="IE189_RS38515"
                     /protein_end="1555"
                     /protein_start="1430"
                     /score="113.8"
                     /specificity="KR activity: inactive"
                     /specificity="KR stereochemistry: C1"
                     /tool="antismash"
                     /translation="GTVLVTGGTGSLGAMVARHLVAGHGVRRLLLVSRRGSDAEGSAEL
                     VAELGESGAVVTVCACDVADRDAVAAVLAAVPVEHPLTGVVHLAGVLDDGVIGALTPER
                     IEGVFAPKVTAVRHLDELTRE"
     CDS_motif       7297..7398
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38515_0011"
                     /evalue="1.00E-08"
                     /label="PKSI-KR_m1"
                     /locus_tag="IE189_RS38515"
                     /protein_end="1465"
                     /protein_start="1431"
                     /score="27.7"
                     /tool="antismash"
                     /translation="TVLVTGGTGSLGAMVARHLVAGHGVRRLLLVSRR"
ORIGIN
        1 gaccgtcata ggtgagtggg tcaaggaggc ggatgtcctc gacggtggtt actggtaccg
       61 gaacctgcgg agccaggtcc gtttcggtcc cgccgtcgca gaccttctgg ccggtggcca
      121 caccgtcttc gtggaggcca gtgcccatcc cgtcctggtc cagccggtca acgagatcgt
      181 ggacgaggcg gatgacacgg tccggctgcg tgctgtcgtg ggtggttcgc tgcgccgtgg
      241 cgagggcggt ctgcaccggc tgttcacctc gatggccgag ctgttcgtcc ggggtgtgcc
      301 ggtggactgg gccgcaatgc tgcctgccgg tgcctcgccg gtcgagcttc cgacgtatgc
      361 cttcgatcga cgtcactact ggttgcagga ggccgacgac gccgacggca gcggctcccc
      421 gggcgaggac atcgacgcgg acttctggag cgccgtcgag cggagcgacg cggactcgct
      481 cgccggactg ctcgccccgg attccgccgg tctgcgcgac gccctgcgca ctgtcgtccc
      541 ggtgctggcg gactggcgcg gcaggcggca gcagcggtcc agcgccgagc gcctccgcta
      601 cgccgtcacc tggcggcccc tggaccggga ggtgacggga gtacccgcgg gccgctggct
      661 cgccgtactg ccgccaggat gcccgaccgg agacgcgacc ggtgtcccgg ccgactccac
      721 ggccggctcc caggtgctcg agctcctcgc ggcgctcgct gcccagggac tcgacatcgt
      781 gcccctggag atcgccacct ctgccttcac ccgcacgggg ttcaccgagc agctgcgcgg
      841 catccgagcc gcgtacgaac cggccggggt cctctccctg ttcgccctcg acggcacgca
      901 ggacgccacg gtcaccgccg ccaggaccct cgcgctgatt caggccctcg gagacgcggg
      961 cgtcgacggg cccctgtggt gtctgacgcg gggcgtggtg aacaccgggg tccaggacac
     1021 cgtcaccgat cctggcgagg ccgcgctctg gggcctgggc cgcgcggtgg ccctggagca
     1081 ccccgaccgg tggggcggcc tggtcgacct cccggcgacc gtcaactccc gcaccatcca
     1141 gtacctcttg ggcgcgctga acggcgcagc gggggacgac cagctcgccg tgcgccgctc
     1201 cggcgtctac agcaggcggc tgacccgcaa gcccgcaccc gaggcgcccg ccgacggcgg
     1261 ctggcagcct cacggcacgg tcctcgtgac gggcggcgcc gaggccctcg gcgcacatgc
     1321 ctcgctctgg ctcgcccggt ccggagtgcg ccgtctcatc atcacgacca cggctcaggc
     1381 gcccgccgac gccgtcacca cgttgctgga cgagctcgcg gccgccgagg tcgcgacgac
     1441 cgtcgtctcc tgcgccgacg ccgaccgtga cacgctcgcc cggctcatcg ccgagacccc
     1501 gcgggagcag ccgctcaccg ccgtcgtgca cgccgccgac atcgcctgga ccggcaacat
     1561 cgccgacacc ggcctcgccg acctcaccga ggtcttcgcg gccaaggtcg acaccgccgt
     1621 atggctcgac gcaatgttca ccgaaaccgg caccggcggg gccccgctcg acgccttcgt
     1681 ggtcttctcc tcgatagccg gaatctgggg cggtggtggc cagggtgtct ccggcgcggc
     1741 caacgcggtc ctggacgccc tggtcgaacg gcgccgtggc cgaggactcg cggccacctc
     1801 gatcgcctgg ggagccctcg acgaaatcgg ccccgccatg gacgaggcga ccctcgcgca
     1861 gctgcgccgc cgcggtgtcc tgccgatgac ccggcagatc gcgacgaccg cgttcgaaca
     1921 ggccgtgcaa gcgcgggaga aggccgtgac ggtcgtcgac atggactggg aggcgttcat
     1981 cccggcgttc acctccggac gggtcagccc gctcttcgcc gatctgcccg aagccgccgc
     2041 cgccctgcgc gcctcccagc ccgatgccga ggacatcggc accacctctt ccctggtgga
     2101 ctccctgcgc aacgtccccg aggcggaaca gaaccgtctc ctgctgcggc tggtgtgcgg
     2161 acaggcctcg accgtcctcg gacacagcag cacggacagc atcggcccgc tccagtcctt
     2221 ccaggaggtc ggtttcgact cgctcgccgc ggtcaacctc cgcaacagcc tgcacgtggc
     2281 caccggccta cgactgcccg cgacgctcat cttcgactat ccgaccccgg acgccctcgt
     2341 cggcttcctg cgctccgaac tgctgacgga agcggacgac gacctggacg cgcgggagga
     2401 cgatctgcgg cgcgtactcg cgcaggtgcc gctcgcccgg ttccgggagg ccggccttct
     2461 cgacacgctg ctcggtctcg tcggtcccga ggacggctcc gcgcccgggg tgacgactcc
     2521 cgtgccggac ccggtggtgc ccgtcgccgc ggacgacgcc gacctgatcg acgtcatgga
     2581 cgtggccgac ctagtgaagc gtgccctggg agacggcacc gacttcctcg gaggcgaccg
     2641 ggagctacca gaccagggga tgccccgcga aggcggccct gcctaatctc gtggaggcag
     2701 ccctcgatat tcgcggatcg tagggcccct gcgctctgat caggtcccgc tcgtccggtt
     2761 ccgggaggcc ggccttctcg acatgccgct cggccccgtc ggtcccgagg acggctccgc
     2821 gcccggggtg acgactcccg tgccggaccc ggtgatgccc gtcgccgcgg acgacgccga
     2881 cctgatcgac gtgatggacg tcgccgacct cgtgcagcgc gtcccgggag acgacaccga
     2941 ccgatccacc gacttgccga catttggttg accgacagcg actaagacgg aggacctgcc
     3001 gagatgacgg caccggacga gcagatcgtc aacgcactgc gtgcctcgct caaggagaac
     3061 acgcggctcc agcaggagaa ccagcgtctc tccgagtcct cggccgaacc catcgcgatc
     3121 gtctcgatgg cctgtcggta cgcgggcggc atacgcaccc ccgaggacct gtggcgggtg
     3181 gtgaccgacg gcaccgacgt ctacaccacc ttccccgagg accgcggctg ggatctggag
     3241 ggcctctacc accccgaccc ggacaacccc ggcacgacgt acgtccgcga gggcgcgttc
     3301 ctgcacgacg ccggtcagtt cgacgccgcg ttcttcggga tatcgccgcg cgaggcgctc
     3361 gcgatggacc ctcagcagcg gcagctcctc gaggtctcct gggaggccct cgaacgagcc
     3421 ggcatcgacc cgcactccgt acgcggcgct gacatcggcg tgttcgccgg catggtccac
     3481 caggactatg cgcccgacct cagcggcctc gaaggcttcc tcagcctgga gcgcgccctg
     3541 ggcaccgcgg gcggtgtcgc ctcgggacgg gtcgcctaca cactcggcct cgaagggcct
     3601 gccgtcaccg tcgacaccat gtgttcctcc tcgatggtcg ccatccacct ggccgcccag
     3661 gcactgcggc gaggcgagtg ctccatggcc ctggccggcg gcgccaccgt gatggcgacc
     3721 cccggcgggt tcgtcggctt cgcccggcag cgcggcctgg ccttcgacgg ccgctgcaag
     3781 tcgtacgcgg cctgcgcgga cggttccagc tgggccgaag gcgcaggcgt gatcctgctc
     3841 gagcggctgt cggaggcacg ccgcaacgga caccgggtcc tcgcggtgat ccgcggctcc
     3901 gccctcaacc aggacggcgc ttccaacggc ctgacggcgc ccaacggacc cgcgcagcag
     3961 cgcgtcatcc gcaaggcgct ggagagcgcc gacctcacca cggccgacat cgacatggtc
     4021 gagggccacg gcaccggcac cgttctcggc gacccgatcg aggcacaggc cctgatcgcc
     4081 acgtacggcc aggaccggtc ggaggaccgg ccgctgtggc tcggctcggt caagtcggtg
     4141 atcggccaca cccaggccgc ctccggtgtg gccgcagtga tcaacatggt gcaggcgctg
     4201 cggcacggcg tcatgcccgc cacccggcac gtcgacgccc ccacctcgca ggtggactgg
     4261 acggcgggct cggtggagct gctgacggag gcccgcccgt ggccggaggt ggggcggccg
     4321 cgccgggccg gtgcgtcgtc gttcggcgcc agcggtacga acgcccacct gatcctggag
     4381 caggcgccgg aagagcccgc cgcggaaacc gccgccggcc cctctccggc tccagcggtc
     4441 gacggcgtgg tgccgctggt gctgtcggcg gccacggccg cctcgctcac cggccaggca
     4501 gagcgtctgc tgtcgttcgt cgagaccgcc ggcgtcgccg gcacggccgc gctcgccgac
     4561 gtggcgaccg cgctggccac cggccgggcg acgctcgccg agcgcgcggt cgtcgtggcc
     4621 gactcgcccg aggaggccct ggcgggcctg ggcgcgctgg cgcggggcga ggccgcacgc
     4681 gcggtggccg tcggcagcgc gtcgacgccc ggcggggccg gaaaggtcgt gctcgtcttt
     4741 cccgggcagg gttcgcaatg gaccggcatg ggccgcgaac tcctcgactc ctcaccggtg
     4801 ttcgcggagc ggatcgccga gtgcgcggcg gcgctcgagc ggtgggtcga ctggtcgctg
     4861 gtggacgtgc tgcgagggga tgctccggcc gaactcctga agcgcgtgga cgtgctacag
     4921 cctgcgagct tcgctgtgat gatcggcctg gccgccgtct ggtcgtcggt cggtgtggtg
     4981 ccggacgccg tggtcggtca ctcgcagggt gagatcgccg ccgcgtgcgt gtcgggcgcg
     5041 ctgtccctgg agaacgctgc tcgcatcgtg gccgtacgca gtcaggtcat cgccgcgagt
     5101 ctggcgggcc gtggcggtat ggcgtcggtg gcgctgtccg aggaagaggc cgtcgagcgg
     5161 ctggagcggt gggccggccg gatcgaggtc gcggcggtca acggcccggt gtccgtggtg
     5221 atcgccggcg acgccgaagc cctcgacgag gccgtcgacg tgctggagga ccaaggcgta
     5281 cgcgtgcgcc gcatcgcggt cgactacgcc tcgcacaccc ggcatgtcga agccgtccag
     5341 gagaccctga gcgaggcctt cgccgacatc cgcagccagg ctcccgccgt gccgttcctc
     5401 tccaccgtga ccggcgagtg gatcagcgag gcgggtgtcc tcgacggcgg ctactggtac
     5461 cgcaacctgc gccgccaggt ccgcttcggt cccgcgatcg ccgacctcct ggccggtggc
     5521 cacaccgtgt tcgtggagtc cagcgcccac cccgtcctgg tccagccgat cagcgagatc
     5581 gtggtccgcg ccgaggccga cgccgtcgtg accggctccc tgcgccgtga ggacggcggc
     5641 ccgcgccgcc tgttcacctc gatggcggag ctcttcgtcc gaggcgtcca cgtcgactgg
     5701 aacggcgtcc tcgcggccgg aaccgacgcc caccgcgtcg acctccccac ctacgccttc
     5761 gaccaccggc actactggat ccagctgacc ggcaccgccg acgacgcggc ctcgctcggc
     5821 ctgaccggca ccgaccaccc gctgctcggc gcggtggtcc cgctgccgca gtccgacggt
     5881 ctggtcctca cctcccggct ctcgctgcgc acccacccgt ggctcgcgga ccacgccatc
     5941 gcaggcgcgg tcgtcgtacc cgggaccatc tacgtcgatc tggcggtccg ggccggcgac
     6001 gagttcggct gcggcgtcct ggacgaactc gtcatcgagt caccgctcgt gctgcccgag
     6061 cagggcggcg tccggatcca ggtcgccgtc agcggcgccg gccccaacgg ctcgcgcacc
     6121 gtggatgtct actccctccg cgaggagacc tccgacgggg cgggacaggg agagtggacc
     6181 cggcatgcca ccggcctgct ctcccaagcc gcccgggccg acgccacgac gcccgccggc
     6241 gacttcgccg tctggccccc ggccggcgcg cagcccgtcg ccgtcgacgg cttctactcg
     6301 gatctggccg cccgcggata cgcctacggt ccggcgttcc agggcctgcg cggtgtgtgg
     6361 cggcgcggcg acgaactgtt cgccgaggcc gtcctgccgc ccgagcagcg cgagaacgcc
     6421 gaccggttcg gcatccaccc ggccctgctc gacgccgctc tgcacaccaa cgccttcgcg
     6481 aaccccgacg acgaccgcag agtgctgccc ttcgcctgga acggtctcgt cctgcacgcc
     6541 cagggcgcct ccgccctgcg cgtacgagtc gccccgagcg gcccggacgc gctctcgttc
     6601 caggccgccg acgagaccgg tggcctcgtc ctgaccatgg actcactggt gtccctgccg
     6661 gtcgccgccg agcagctcgg ctccggcgcg gccggccggg acagccgcga ctcgctgttc
     6721 cgcgtggagt ggaccctcct gcccgccccc gagggcgccg ccgcccccgc ctcctgggcg
     6781 tctctcgccg gcgtcgacga cgtgacggcg ctcgccgccc gcgagaccct gccgccggtc
     6841 gccgtcctca cggcgacgac cggcgacggc gaggatccgc tcggcctcac cacccgcgtc
     6901 gtggccgtgg tccaggcatg gctggccgcc gcacccgccc tggacgccgt acctctcgtc
     6961 gtcgtgaccc ggggcgcggt gcccgcgggc ggtgacgccg acgtcacgga cccggccggt
     7021 gccgcggtct ggggcctgat ccgcgccgcc caggccgaga accccgatcg catcgtgctc
     7081 gtcgacctcg accccgccgg gtccgccgga gcgggtccgg tcctcgggcc cgtgctggcc
     7141 accggggagc cgctggtcgc cgtccgcggc acggccctgt ccgccccgcg tctggtacgc
     7201 gcggccgccg tcgagaccga gcgagctgcc gacgccgacg ctggcgccga gaccaggacc
     7261 aggaccgaga gtgcgtacgc cttcgccccc gggggcaccg tcctggtcac gggcgggacg
     7321 ggttccctgg gtgccatggt cgcccggcac ctggtggccg ggcacggtgt gcggcgtctg
     7381 ctgctggtca gtcggcgtgg ttccgatgcg gagggctcgg ctgaactggt tgccgagctg
     7441 ggtgagtcgg gtgcggtggt gacggtctgc gcgtgtgatg tcgccgatcg ggacgcggta
     7501 gcggccgttc tggcggcggt gcctgtcgag catccgctga ccggtgtcgt ccatctcgcc
     7561 ggggtcttgg acgacggggt gatcggcgcg ctgacgccgg aacggatcga aggcgtcttc
     7621 gccccgaagg tcaccgccgt ccgccacctg gacgagctga cccgtgagct cgccccgcag
     7681 ttggcctcgt tc
//
