LOCUS       NZ_BMSI01000033         9791 bp    DNA     linear   CON 02-AUG-2024
DEFINITION  Streptomyces asoensis strain JCM 4185 sequence33, whole genome
            shotgun sequence.
ACCESSION   NZ_BMSI01000033
VERSION     NZ_BMSI01000033.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces asoensis
  ORGANISM  Streptomyces asoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649075.1-RS_2024_08_02
            Annotation Date                   :: 08/02/2024 04:45:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,813
            CDSs (total)                      :: 7,736
            Genes (coding)                    :: 7,563
            CDSs (with protein)               :: 7,563
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 53 of 173
            Pseudo Genes (incomplete)         :: 140 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 28 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:21:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 9791
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSI01000033.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     gene            <1..101
                     /locus_tag="IE189_RS39435"
     source          1..9791
                     /culture_collection="JCM:4185"
                     /db_xref="taxon:249586"
                     /mol_type="genomic DNA"
                     /organism="Streptomyces asoensis"
                     /strain="JCM 4185"
                     /submitter_seqid="sequence33"
                     /type_material="type strain of Streptomyces cacaoi subsp.
                     asoensis"
     region          1..9791
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="T1PKS"
                     /region_number="1"
                     /rules="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS or
                     hyb_KS or itr_KS or tra_KS))"
                     /tool="antismash"
     cand_cluster    1..9791
                     /SMILES=""
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="cds(PKS_AT and (PKS_KS or ene_KS or
                     mod_KS or hyb_KS or itr_KS or tra_KS))"
                     /kind="single"
                     /product="T1PKS"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..9791
                     /aStool="rule-based-clusters"
                     /category="PKS"
                     /contig_edge="True"
                     /core_location="[7985:>9791](+)"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      7986..>9791
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="cds(PKS_AT and (PKS_KS or ene_KS or mod_KS
                     or hyb_KS or itr_KS or tra_KS))"
                     /neighbourhood="20000"
                     /product="T1PKS"
                     /protocluster_number="1"
     CDS             <5..101
                     /codon_start=3
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018088768.1"
                     /locus_tag="IE189_RS39435"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="DUF6243 family protein"
                     /protein_id="WP_229901629.1"
                     /transl_table=11
                     /translation="AADQKAELVRKMREKTGTGEDAGQSGDNSAQS"
     misc_feature    424..441
                     /note="TFBS match to ArcA, Repressor of carbon oxidation,
                     confidence: weak, score: 15.57"
                     /tool="antismash"
     misc_feature    complement(618..632)
                     /note="TFBS match to CodY, Regulator of stationary phase
                     and virulence in Gram-positive bacteria, confidence: weak,
                     score: 16.05"
                     /tool="antismash"
     gene            954..3827
                     /locus_tag="IE189_RS38475"
                     /old_locus_tag="GCM10010496_78060"
     CDS             954..3827
                     /codon_start=1
                     /gene_functions="regulatory (smcogs) SMCOG1149: LuxR family
                     transcriptional regulator"
                     /gene_kind="regulatory"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /locus_tag="IE189_RS38475"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_78060"
                     /product="LuxR family transcriptional regulator"
                     /protein_id="WP_189928724.1"
                     /transl_table=11
                     /translation="MLIARELELAALRIALRDSAAGRARTIFVEGAVGYGKTELLEALA
                     VLSCEAGAIVLRATGMPSEESVPLGLVRQLVHSEALPTETKIWLERLLDEEEGSTGPGL
                     PDGTAQGSGARMQKFWTALRTVARDRLVVIGVDDLQHADSTSLQYLLYIAGRSRTSRLL
                     MVFTEALYYRQQDTAYRTELLRQPNFRRIRLEGLGPDAVARFLAQHQPRLPVDEETTEE
                     FYAVTRGNPLLLRALLEERGTVTGTSGDCPDPAPGETFAHAVLTCLDRSGPTAAKVADG
                     LAVLGAATTLDLLSRLCRLPRTSAAQGLRALHAAGVVDACHFQHPAVSSQVLDGMDPEH
                     RQQLHRRAATLLYADGAPASAVAPHLLALERIDEPWVVAVLCDAAEEALADDEDGVAVA
                     CLELAHRVCDEPDRRIEIKVRTSVILRRNHPAAAETAAEELLAAVRAGLTPARYTTAIA
                     DLFLAHRRLEEAHEVLVGGARDTGDAMVPSGAGGSGRAAGGGVHAGPAAVAPERGTATA
                     LMTSIVERRYPWAESRMAGIPPQSREVRALPARPGNEPPSVRVSQQPWRLLLGPWRGDF
                     VTAAEEMLQRSALTDATMEPITSAIKCLLFSGQTKRARHWCDVLLRNTARRGAGGWHAM
                     FAGLRAEVALAEGNPEEAERCIDAGLSRITEHKRSVLACGLIAVQVMAQTAMGKYEAGA
                     RKLNEPALQAVYASAYGLTYLRARGHYNLATNSLDAALDDFMAVGRTAQRWGLDQPVWV
                     PWRSDLAEALLGTGEYKEAERLVLEQISRLDDANPRIHGISLRLLAEASAPGERLGLLT
                     RAVAQLRLSGDRLELARALCDLGETHKRLGDPAQAAMASRRAWELARECGAEPLCARIR
                     LAHGDDDWGTRPEKTVVDTREAKLSDSEKRVAALAACGYTNRDISSQLYITVSTVEQHL
                     TRVYRKLKIGGRQQLPIDLQFDLPETA"
     misc_feature    972..974
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     misc_feature    1074..1076
                     /note="tta leucine codon, possible target for bldA
                     regulation"
                     /tool="antismash"
     gene            complement(3908..5647)
                     /locus_tag="IE189_RS38480"
                     /old_locus_tag="GCM10010496_78070"
     CDS             complement(3908..5647)
                     /EC_number="1.1.1.35"
                     /GO_function="GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA]"
                     /GO_function="GO:0070403 - NAD+ binding [Evidence IEA]"
                     /GO_process="GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1095: 3-hydroxybutyryl-CoA dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016470667.1"
                     /locus_tag="IE189_RS38480"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_78070"
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="WP_189928725.1"
                     /transl_table=11
                     /translation="MAQTFTRTAVIGVGAVGAALVDLLARAGLPVTAVEADEPSLAAGR
                     RRVAGRAADGAEPPGVRWSTRVADVAGADLVVEAVSERLDAKTAVLGEAARVCGPGTVF
                     ATTTSGQSVTEIASLCGTMTRTVGLHLFPAVPTTAVEVVRTPLTERPVQEAVVDLARRL
                     GHTPVGVADRPGFIGGALAMAYLNSAATMYEQRYASREDIDTAMTLGCGLPMGPLAQLD
                     VMGLDVARDTLELLYERTGDRAYAPAPLLSHYVAAGLLGRKAGRGFYDYGSGGATVASS
                     AVGSPSAEGGPSTRPVRTVGVVGSGTMGAGIAEVCIRAGHATVLVARSDAKAKGAVLAV
                     ERSLERAVGRGKVTRQDADAALGRLVAVSQLEALDECDLVVEAVVEDLTVKRGLFADLG
                     RTCRPDAVLASSTSGLPIIECATATGRPENVLGMHFFNPAPVMRLVEVVRTALTSDRTA
                     GIAHAAAAAMGKRPVGCADRAGFIVNALLFPYLNRAIRLVEEQAITPDDVDAVMTGGHG
                     YPMGPLKLLDVVGLDVSLQIQRTLHDTFLDASLAPARYLEHLVRAGHLGNKTGQGFRVH
                     AGR"
     gene            complement(5762..6214)
                     /locus_tag="IE189_RS38485"
                     /old_locus_tag="GCM10010496_78080"
     CDS             complement(5762..6214)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /locus_tag="IE189_RS38485"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_78080"
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="WP_189928726.1"
                     /transl_table=11
                     /translation="MPDEATAKQLILDHCRKINEGDVEGLLKLYAEDCCFEDPVGNGKQ
                     YGLKALSQRAAAAVFSGAYEDAASPVASKDGRWAAVPMVSTFNYLPLAGPAMAQSGLLP
                     SDPPPDPEHKMIRVDIVMVIRVNDDGLVDQMQAFYGRGDATVIDKG"
     gene            complement(6317..7507)
                     /locus_tag="IE189_RS38490"
                     /old_locus_tag="GCM10010496_78090"
     CDS             complement(6317..7507)
                     /GO_function="GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /GO_function="GO:0005506 - iron ion binding [Evidence IEA]"
                     /GO_function="GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /GO_function="GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) p450"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1007: cytochrome P450"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /locus_tag="IE189_RS38490"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_78090"
                     /product="cytochrome P450"
                     /protein_id="WP_189928727.1"
                     /sec_met_domain="p450 (E-value: 6.8e-28, bitscore: 86.2,
                     seeds: 50, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEQALALTPPSAADGGAALFDWLRVMRDEAPVVRGSNGSWYVFR
                     YADVQHVLSGYADFSSDPTPVSPKAAEVAKGDLAQIDPPVHHHLRRQISRVFTPRMVAG
                     LEPRIREIGTALLDAAGDSGEMDLVRQLTHPLPVIVIAELLGVPAEDRPVFRHWADRII
                     AAKIAGVDDPETYERLRSAIEEMDYYLEGQLEERRARPGDDLLSRMVTSELDGEPITGA
                     EAVNFARLLLIAGHITTTMMLSSSVLSFDEFPEAAAELRADRSLIPGALEEVMRYRPPF
                     TITGRYTTRDVELSGVTVPERSIVIPWLVSANRDERQFPDPDVFDIHRSPNAHVAFGKG
                     IHFCIGAPLARLEGRVALELLLDRFSDVRLDRDVPLRFYEQNFFGVKSLPVSVKRA"
     gene            7986..>9791
                     /locus_tag="IE189_RS38495"
     CDS             7986..>9791
                     /NRPS_PKS="Domain: PKS_KS(Hybrid-KS) (13-438). E-value:
                     7.8e-170. Score: 557.3. Matches aSDomain:
                     nrpspksdomains_IE189_RS38495_PKS_KS.1"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     hyb_KS"
                     /gene_functions="biosynthetic (rule-based-clusters) T1PKS:
                     PKS_AT"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) ketoacyl-synt"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1022: Beta-ketoacyl synthase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012381764.1"
                     /locus_tag="IE189_RS38495"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="type I polyketide synthase"
                     /protein_id="WP_189928728.1"
                     /sec_met_domain="hyb_KS (E-value: 7.8e-176, bitscore:
                     573.7, seeds: 33, tool: rule-based-clusters)"
                     /sec_met_domain="ketoacyl-synt (E-value: 8.9e-86, bitscore:
                     276.2, seeds: 77, tool: rule-based-clusters)"
                     /sec_met_domain="PKS_AT (E-value: 2.5e-17, bitscore: 51.8,
                     seeds: 1682, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTPSGDGPAGDDAIAVVGLACRLPGAPTPTAFWELLRAGAHAVRE
                     VPARRFGAAAAADEGDDAHDHRAPRFGAFLDQEQIETFDAAFFGISPREAALMDPQQRL
                     ALELGWEALEDAGLPPADLAGQRSGVFVGVIADDYATLSARLGADALGPHSLTGLHRSM
                     IANRVSYVLGLRGPSMTVDTGQSSSLVSVHLACESLRTGESTVALAGGVNLNLLPETTE
                     RVARFGALSPDGRCHTFDARANGYVRGEGGAVLVLKRLSQAVADGDTIHCLLLGSAVNN
                     DGGGESLTAPHRAAQEEVLRLAHQRAGTDPADVQYVELHGTGTKVGDPVEAAALGAVVG
                     TAHEPGRPVMVGSVKTNVGHLEGAAGITGLLKTVLSLRHRLLPASLNYETPNPDIPLDR
                     LNLSVQRTTGPWPADGRPLLAGVSSFGMGGTNCHVVAAEWTGNREATAPHSGTAPFVHA
                     PVTCWPLSGPSRTGLRGQAARLAAFADERTGVSLPDVGWSLATGRAALEHRAVVLAGDR
                     DAAVGVLRSVAEGVPSAGVVSGVVSGEQGRVVFVFPGQGSQWVGMGGGLWESSPVFRER
                     LQECDAALSSLVDWSVTDVVRGVPDA"
     aSDomain        8025..9299
                     /ASF="found active site cysteine: False, scaffold matched
                     GSSS: True"
                     /ASF="found active site histidines: True"
                     /aSDomain="PKS_KS"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS38495_PKS_KS.1"
                     /domain_subtypes="Hybrid-KS"
                     /evalue="7.80E-170"
                     /label="IE189_RS38495_PKS_KS.1"
                     /locus_tag="IE189_RS38495"
                     /protein_end="438"
                     /protein_start="13"
                     /score="557.3"
                     /tool="antismash"
                     /translation="IAVVGLACRLPGAPTPTAFWELLRAGAHAVREVPARRFGAAAAAD
                     EGDDAHDHRAPRFGAFLDQEQIETFDAAFFGISPREAALMDPQQRLALELGWEALEDAG
                     LPPADLAGQRSGVFVGVIADDYATLSARLGADALGPHSLTGLHRSMIANRVSYVLGLRG
                     PSMTVDTGQSSSLVSVHLACESLRTGESTVALAGGVNLNLLPETTERVARFGALSPDGR
                     CHTFDARANGYVRGEGGAVLVLKRLSQAVADGDTIHCLLLGSAVNNDGGGESLTAPHRA
                     AQEEVLRLAHQRAGTDPADVQYVELHGTGTKVGDPVEAAALGAVVGTAHEPGRPVMVGS
                     VKTNVGHLEGAAGITGLLKTVLSLRHRLLPASLNYETPNPDIPLDRLNLSVQRTTGPWP
                     ADGRPLLAGVSSFGMGGTNCHVVAAE"
     CDS_motif       8511..8558
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38495_0001"
                     /evalue="1.40E-04"
                     /label="PKSI-KS_m3"
                     /locus_tag="IE189_RS38495"
                     /protein_end="191"
                     /protein_start="175"
                     /score="14.2"
                     /tool="antismash"
                     /translation="GPSMTVDTGQSSSLVS"
     CDS_motif       8700..8738
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38495_0002"
                     /evalue="1.60E-05"
                     /label="PKSI-KS_m4"
                     /locus_tag="IE189_RS38495"
                     /protein_end="251"
                     /protein_start="238"
                     /score="17.5"
                     /tool="antismash"
                     /translation="FDARANGYVRGEG"
     CDS_motif       8931..8978
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38495_0003"
                     /evalue="6.30E-05"
                     /label="PKSI-KS_m5"
                     /locus_tag="IE189_RS38495"
                     /protein_end="331"
                     /protein_start="315"
                     /score="14.7"
                     /tool="antismash"
                     /translation="YVELHGTGTKVGDPVE"
     CDS_motif       9039..9083
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38495_0004"
                     /evalue="2.30E-04"
                     /label="PKSI-KS_m6"
                     /locus_tag="IE189_RS38495"
                     /protein_end="366"
                     /protein_start="351"
                     /score="13.4"
                     /tool="antismash"
                     /translation="GSVKTNVGHLEGAAG"
     CDS_motif       9630..9683
                     /aSTool="nrps_pks_domains"
                     /database="abmotifs"
                     /detection="hmmscan"
                     /domain_id="nrpspksmotif_IE189_RS38495_0005"
                     /evalue="4.60E-08"
                     /label="PKSI-AT-mM_m1"
                     /locus_tag="IE189_RS38495"
                     /protein_end="566"
                     /protein_start="548"
                     /score="25.0"
                     /tool="antismash"
                     /translation="VFVFPGQGSQWVGMGGGL"
ORIGIN
        1 gtgccgccga ccagaaggcg gagctggtgc gcaagatgcg cgaaaagaca ggcacaggcg
       61 aggacgctgg gcagtcgggc gacaacagcg cacagagctg acgcaccgcc gccgcagggt
      121 gacaccgcac ggggcagggc tcggaccgcg acacgtggtc cgagccctgc tgccgtaccg
      181 ggcgcggccc gccctgctca gctttcggcc gagcacccgc gtctcagacc aaccgagcca
      241 gccgcagatc caccagcccg tcaccgccac caccccgagc tgcaccatct tggtgttgtc
      301 ggacgagctc ccccgcggga tgtcgccggg cacggcactg gtcaggtggc aggggaaccg
      361 gtagggcccc tcgctcgggc gtcctcaccg gctcggcgcg ccggcgccgc cgaatttacg
      421 cgctggtaaa gaggtggtta agaaatcgga taccgcttgt gtggcgcagc gcacctcgtc
      481 tagggatccg ggccgaacgc gacgcgcccc cggacttcga tagggggata cccctaactt
      541 acggcgcgcg cccgtcccgt catcggccaa tcacccgacc gcatgtgaga ggcggcccac
      601 aactgttcgt cagcgggcat ttggtgagaa ttcagaccgg tgcctcttcg tggccgtaca
      661 gcggaatgtg tggttcccat ggaaacggga ccgtctactt cggtatcaag cggcagattg
      721 aggtcgcggt atccgtgttt ccgtcgctgg ccaggcggag gggtccggtc cggggatggt
      781 tgacagtcgg atgagcccga gggtttcatc gatctgtgga cacgggcggg ggaatcagcc
      841 gtgcggacat ccgtctcatc cgggcggagc ggccgaaacc accgccgaag cagaaggcgt
      901 tgacgctctg tcattgccgc cggtccatcg gtgaaggggg atccggattc cgtatgctga
      961 tagccaggga attagaactc gccgcactga gaatagctct gcgcgacagt gcggcgggaa
     1021 gagcgagaac catttttgtc gagggagccg tcggatatgg caagaccgag cttttagaag
     1081 ccctggcggt attgtcatgt gaagccggag caatcgttct ccgagccacc ggaatgccgt
     1141 cggaggaaag tgttccgctg ggattggtgc ggcaactcgt ccacagcgaa gccctgccca
     1201 ccgaaaccaa gatctggctc gaacgactgc tggacgagga agaggggtcg accggccccg
     1261 gcctgcccga cgggacggcc cagggcagcg gcgcgagaat gcagaagttc tggacggcgc
     1321 tgcgcaccgt cgcgcgcgac cggctcgtgg tcatcggggt cgacgacctc cagcacgccg
     1381 acagcacctc cctgcaatac ctgctctaca tagcgggccg ttcgcgcacg agccggctgc
     1441 tgatggtgtt cacggaggcc ctctactacc gacagcagga caccgcctac cgcaccgaac
     1501 tcctgcgcca gccgaacttc cgcagaatcc gcctggaagg cctcggcccc gacgcggtgg
     1561 cccgtttcct cgcccagcac cagccccggc tgccggtgga cgaggaaacg accgaggagt
     1621 tctacgccgt cacccgcggc aatccgctgc tgctgcgcgc cctgctggag gagcgcggga
     1681 cggtcacggg cacgtccggc gactgtccgg acccggcgcc cggggagacg ttcgcccatg
     1741 ccgtcctcac ctgcctggac cgcagcgggc ccacggccgc caaggtcgcc gacgggctgg
     1801 ccgtcctggg cgcggccacc accctcgacc tgctcagcag gctgtgccgg ctgccccgta
     1861 cctcggccgc ccagggcctg cgcgccctgc acgccgccgg cgtggtcgac gcctgccact
     1921 tccagcaccc ggccgtgagc agccaggtcc tggacggcat ggaccccgag caccgccagc
     1981 agctccaccg cagggcggcc accctgctgt acgccgacgg cgccccggcc tccgccgtcg
     2041 ccccccacct gctggcgctg gaacgcatcg acgagccgtg ggtggtcgcc gtcctgtgcg
     2101 acgccgcgga ggaggcgctc gccgacgacg aggacggcgt ggcggtcgcc tgcctggagc
     2161 tggcccaccg ggtctgcgac gagcccgacc ggcgcatcga gatcaaggtg cgcacctcgg
     2221 tcatcctgcg gcgcaaccac ccggccgccg cggagacggc cgccgaggaa ctgctcgcgg
     2281 ccgtgcgggc gggccttaca ccggcccggt acacgacggc gatcgcggac ctgttccttg
     2341 cccaccggcg gctggaggag gcgcacgagg tcctcgtcgg cggggcccgc gacaccgggg
     2401 acgccatggt gccgtccggg gccggcgggt ccggacgtgc cgccggcggt ggcgtgcacg
     2461 ccggtccggc cgccgtcgcg ccggaacggg gcacggcgac tgccctgatg acctccatcg
     2521 tcgaacgccg ctacccgtgg gcggagagcc ggatggccgg catcccgccc cagagccgcg
     2581 aggtacgggc cctgccggcc cggccgggca acgagccgcc gtccgtccgg gtctcccagc
     2641 agccgtggag gctgctcctc ggcccctggc gcggcgactt cgtcaccgcc gccgaggaga
     2701 tgctgcaacg ctccgcgctc accgacgcca cgatggagcc catcaccagc gccatcaagt
     2761 gcctgctgtt ctccgggcag acgaaacggg cccgccactg gtgcgacgtg ctcctgagga
     2821 acaccgcccg gcgcggcgcg ggcggctggc acgccatgtt cgccgggctg cgcgccgagg
     2881 tcgccctcgc ggagggcaac cccgaggagg ccgagcgctg catcgacgcg gggctgagcc
     2941 gtatcaccga gcacaagcgc agcgtgctgg cctgcggcct catcgccgtc caggtgatgg
     3001 cccagaccgc gatgggcaag tacgaggccg gcgcccgcaa gctcaacgag cccgccctgc
     3061 aagcggtgta cgcgagcgcc tacggcctga cgtacctgcg ggcccggggc cactacaacc
     3121 tggccacgaa cagcctcgac gcggcgctgg acgacttcat ggccgtgggc cgcacggcgc
     3181 agcgctgggg cctcgaccag ccggtgtggg tgccctggcg cagcgacctg gccgaggcgc
     3241 tgctcggcac cggcgagtac aaggaggccg agcggctggt cctggagcag atctcccgcc
     3301 tcgacgacgc caacccccgg atccacggca tctccctgcg gctgctcgcc gaggcgtccg
     3361 caccggggga gcggctgggg ctgctcaccc gcgccgtggc ccaactgcgc ctgtccggtg
     3421 accgtctgga gctggcacgg gccctgtgcg acctgggcga gacccacaag cggctcgggg
     3481 atccggcgca ggccgcgatg gcgtcgcggc gggcctggga actggccagg gagtgcggcg
     3541 ccgagccgct gtgcgcgcgg atccgcctcg cccacgggga cgacgactgg ggcacccgcc
     3601 cggagaagac cgtggtggac acccgcgagg ccaagctcag cgactcggag aagcgggtgg
     3661 cggcgctggc cgcctgcggc tacaccaaca gggacatctc ctctcagctg tacatcaccg
     3721 tcagcaccgt cgaacagcac ctgacgcggg tctaccggaa gttgaagatc ggcggtcgcc
     3781 aacagctgcc gatcgacctt cagttcgacc tgcccgagac cgcgtgacat gaggccgcgg
     3841 cggcggttcc cccacgcgcg ggggcaccgt cccggcggcc ccgcgcgtgg gcggcccggc
     3901 gggcggctca gcggcccgcg tggacccgga acccctgccc cgtcttgttc cccagatgac
     3961 ccgcccgcac caggtgctcc aggtagcggg ccggggcgag cgacgcgtcg aggaacgtgt
     4021 cgtgcagcgt ccgctggatc tgcagcgaca cgtcgagtcc gaccacgtcc agcagcttca
     4081 gcggccccat cggatagccg tggccgcccg tcatcacggc gtccacgtcg tccggggtga
     4141 tggcctgctc ctccacgagc ctgatcgccc ggttcaggta ggggaacaac agggcgttga
     4201 cgatgaaccc ggcccggtcc gcgcagccca ccggacgctt gcccatcgcg gcggccgcgg
     4261 cgtgggcgat gcctgccgtc ctgtcggagg tgagcgccgt gcggaccacc tccaccagcc
     4321 gcatcaccgg agccgggttg aagaagtgca tgccgagcac gttctccggg cggcccgtgg
     4381 ccgtggcgca ctcgatgatc ggcagcccgg acgtcgacga ggccagcacc gcgtccggcc
     4441 ggcacgtccg gcccagatcg gcgaacaggc cccgcttgac cgtcaggtcc tcgaccaccg
     4501 cctcgacgac caggtcgcac tcgtcgagcg cctccaactg ggagacggcc accagcctgc
     4561 ccagggccgc gtccgcgtcc tgccgggtca ccttcccccg gccgaccgcc cgttccaggg
     4621 accgctcgac cgccagcact gcccccttgg ccttggcgtc gctgcgcgcg acgagcaccg
     4681 tggcgtggcc ggccctgatg cacacctcgg cgatgcccgc gcccatcgtc ccggacccca
     4741 ccacgcccac cgtgcgcacg gggcgcgtgg acgggccgcc ctcggcggag ggggagccga
     4801 ccgccgagga cgcgacggtc gcaccgccgg acccgtagtc gtagaacccg cgccccgcct
     4861 tccgcccgag gagcccggca gccacgtagt gcgacagcag cggcgcaggc gcgtaggccc
     4921 ggtcgccggt ccgctcgtac agcagctcca gcgtgtcccg ggccacgtcg aggcccatga
     4981 cgtcgagctg cgccagagga cccatcggaa gcccgcagcc cagcgtcatc gccgtgtcga
     5041 tgtcctcccg ggaggcgtag cgctgctcgt acatcgtcgc cgcgctgttg aggtacgcca
     5101 tcgccagtgc gccgccgatg aagcccgggc ggtccgcgac ccccaccggg gtatggccca
     5161 ggcgccgcgc caggtccacc accgcctcct ggacgggccg ctcggtcaag ggggtgcgca
     5221 ccacctccac cgccgtcgtg ggcaccgccg ggaacagatg caggccgacg gtgcgggtca
     5281 tggtgccgca cagcgaggcg atctcggtga ccgactgccc gctcgtggtg gtggcgaaca
     5341 cggtgcccgg cccgcacacc cgggccgcct ccccgagcac ggcggtcttg gcgtccagcc
     5401 gttcggacac cgcttcgacg accagatcgg caccggcgac gtccgccacc cgggtgctcc
     5461 agcgcactcc cgggggttcg gcaccgtcgg cggcgcgtcc ggcgacccgc cgcctgccgg
     5521 ccgcgaggga cggttcgtcg gcctccacgg cggtgaccgg cagccccgcc cgggccagca
     5581 ggtccacgag cgcggcgccg accgcgccca cccctatcac cgctgtccgc gtgaacgtct
     5641 gagccatggt ggttgggtcc cttcggttcc gttgtccggt cggtgcgcag acgctgggac
     5701 cgcggctcgg ctctccgtcc gggcggcgaa ggcggcgggg atcccctgcg cccgagtggg
     5761 gtcagccctt gtcgatgacg gtggcgtcac cgcggccgta gaaggcctgc atctggtcga
     5821 cgagaccgtc gtcgttcacc cggatcacca tcacgatgtc gacccggatc atcttgtgct
     5881 ccgggtccgg cggcggatcc gacggcagca ggccgctctg cgccatcgcc ggcccggcga
     5941 gcggcaggta gttgaacgtc gagaccatgg ggaccgcggc ccagcggccg tccttggacg
     6001 ccaccggcga cgccgcgtcc tcgtaggcgc ccgagaagac ggccgccgcg gcgcgctggc
     6061 tcagcgcctt gagcccgtac tgcttgccgt tgccgaccgg gtcctcgaag cagcagtcct
     6121 ccgcgtacag cttgagcagg ccctccacgt ccccctcgtt gatcttccgg cagtggtcca
     6181 ggatgagctg cttggcggtc gcctcgtcgg gcatggggtc tccggtccct ctcgcagtga
     6241 gcctcgggtg tcgggcccgg ggtctggtgt ctcgggacgg cggggtcccg gtgccttgcc
     6301 ggtggcggcg ggtgccctag gcgcgcttca ccgacaccgg gagcgacttc acgccgaaga
     6361 agttctgctc gtagaaacgc aacggcacgt cacggtcgag ccggacgtcc gagaaccggt
     6421 ccagcaacag ctcgagggcg acccggccct ccaggcgggc cagcggggcg ccgatgcaga
     6481 agtggatgcc cttgccgaac gccacgtgcg cgttgggcga acggtgaatg tcgaacacgt
     6541 ccgggtcggg gaactgccgc tcgtcgcggt tcgccgacac cagccacgga atgacgatcg
     6601 agcgctccgg aacggtcacc ccggacagtt ccacgtcccg ggtggtgtag cggccggtga
     6661 tggtgaacgg cggccggtag cgcatcactt cctccagcgc accggggatc agcgagcggt
     6721 ccgcccgcag ttccgccgcg gcctcgggga actcgtcgaa ggacaggacg gagctgctga
     6781 gcatcatcgt ggtggtgatg tgccccgcga tcagcagcag tcgcgcgaag ttgaccgcct
     6841 cggcccccgt gatcggctcc ccgtccagct cgctcgtcac catccggctc agcaggtcgt
     6901 ccccgggacg ggcccgccgt tcctcgagct gcccctccag gtagtagtcc atctcctcga
     6961 tcgcggacct cagccgctcg taggtctccg ggtcgtccac gccggcgatc ttcgccgcga
     7021 tgatgcggtc cgcccagtgc cggaacaccg gacggtcctc cgccggtacg cccagcagtt
     7081 cggcgatcac gatcaccggc agcggatgcg tcaactgccg caccaggtcc atttcgccgg
     7141 agtctccggc ggcgtccagc agcgcggtgc cgatctcgcg gatgcgcggc tccaggcccg
     7201 ccaccatccg cggggtgaac acccggctga tctgccgccg caggtggtgg tggacgggcg
     7261 ggtcgatctg cgccaggtca cccttggcca cctccgccgc cttgggcgag accggcgtcg
     7321 gatcggagga gaagtcggcg tacccggaca gcacgtgctg cacgtcggcg tagcggaaca
     7381 cgtaccaact gccgttgctg ccgcggacga cgggagcctc gtcgcgcatc acgcgcaacc
     7441 agtcgaacaa ggccgctcca ccgtcggcag ccgagggagg agtgagggcg agagcctgtt
     7501 cggacatgtg aggactcctt gcgtccggac gagatggcag ggacgagccg gcgacggaag
     7561 aagaaatgag gaaagaggaa gaagaaagca ggtgaccggg aggagaaggg gaggtgcggg
     7621 tccttcccca ccgtcgttcc gcttgtcccg gaaaaggaaa gccgagttct gcgaacgggc
     7681 gggatgcatc ccctggaacg cacgaaccgt atccgagccg gcccgggcca gacatcccca
     7741 gattccccgt agggggacgc ccggacgacg taggggtggg ggccgccgta gctaggggcc
     7801 cgacagcccc aggaaccggt cgacacacac accgccgtcc tgctgggatt cggggaaacc
     7861 aacgcatcac ctcgacaccc acccgttcgg attccctctt ccatccggcc cgatcgcggt
     7921 ctcgacgcaa ttcccgggga gctctggaga cagtgttggc ggacaacaag gcgcgcgaga
     7981 attccatgac cccgtccggc gacgggcccg ccggcgacga cgcgatcgcc gtcgtgggac
     8041 tcgcctgccg cctccccgga gcccccaccc ccaccgcctt ctgggagttg ctgcgcgcgg
     8101 gcgcccacgc cgtccgtgag gtccccgccc gacgcttcgg cgccgcggcc gccgccgacg
     8161 agggcgacga cgcgcacgac caccgcgccc cacgcttcgg cgccttcctc gaccaggaac
     8221 agatcgagac cttcgatgcc gcgttcttcg gcatctcgcc ccgcgaagcc gcgctgatgg
     8281 acccccagca gcgcctggca ctggaactcg gctgggaggc gctggaggac gccggactgc
     8341 ccccggcgga cctcgccggg cagcgctccg gcgtgttcgt cggcgtcatc gccgacgact
     8401 acgccacgct ctccgcccgc ctcggcgccg acgccctcgg cccccactcc ctgaccgggc
     8461 tgcaccgcag catgatcgcc aaccgggtct cgtacgtcct gggcctgcgc ggtcccagca
     8521 tgaccgtcga caccggccag tcgtcctccc tggtctcggt ccacctcgcc tgcgagagcc
     8581 tgcgcaccgg cgagagcacc gtcgccctcg ccggcggcgt caacctcaac ctcctccccg
     8641 agaccaccga acgcgtcgcc cgcttcggcg ccctctcccc ggacggccgc tgccacacct
     8701 tcgacgcccg cgccaacggc tacgtgcgcg gcgagggcgg cgccgtcctg gtgctcaagc
     8761 gcctctcgca ggccgtcgcc gacggcgaca ccatccactg cctgctgctc ggcagcgccg
     8821 tcaacaacga cggcggcggc gagtccctca ccgcccccca ccgcgccgcc caggaggagg
     8881 tcctgcgcct ggcccaccag cgggccggca ccgaccccgc ggatgtccag tacgtcgaac
     8941 tccacggcac cggcaccaag gtcggcgacc ccgtcgaggc cgccgcgctc ggcgcagtcg
     9001 tcggcaccgc ccacgagccc ggccgccccg tcatggtcgg ctccgtcaag accaacgtgg
     9061 gccacctgga gggagccgcc ggcatcaccg ggctcctcaa gacggtgctc agcctccggc
     9121 accggttgct ccccgccagc ctcaactacg agacgcccaa ccccgacatc cccctcgacc
     9181 ggctcaacct gagcgtccag cggaccaccg gcccctggcc cgccgacggc cggccgctgc
     9241 tcgccggggt gtcctcgttc ggcatgggcg gcaccaactg ccacgtcgtg gccgccgaat
     9301 ggaccggcaa ccgcgaggcc accgccccac acagcggcac cgcaccgttc gtccacgccc
     9361 ccgtgacgtg ctggccgctg tccggcccga gccgcacggg cctgcggggt caggcggcgc
     9421 ggctcgccgc gttcgcggac gagcgtacgg gcgtgtcgct gccggacgtg ggttggtcgc
     9481 tggcgacggg ccgggccgcg ctcgagcacc gtgcggtggt tctggccggt gatcgggatg
     9541 ctgctgttgg ggtgttgcgg tcggtggcgg agggtgtgcc ttcggccggt gtggtgtcgg
     9601 gtgtggtgtc cggggagcag ggccgggtgg tgttcgtgtt cccgggtcag gggtcgcagt
     9661 gggtggggat gggtgggggg ttgtgggagt cgtcgccggt gttccgggag cggcttcagg
     9721 agtgtgatgc ggcgctgtct tcgctggtgg actggtcggt gactgatgtg gtgcggggtg
     9781 tgccggatgc g
//
