LOCUS       NZ_BMSI01000022        18542 bp    DNA     linear   CON 02-AUG-2024
DEFINITION  Streptomyces asoensis strain JCM 4185 sequence22, whole genome
            shotgun sequence.
ACCESSION   NZ_BMSI01000022
VERSION     NZ_BMSI01000022.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces asoensis
  ORGANISM  Streptomyces asoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 113414)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649075.1-RS_2024_08_02
            Annotation Date                   :: 08/02/2024 04:45:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,813
            CDSs (total)                      :: 7,736
            Genes (coding)                    :: 7,563
            CDSs (with protein)               :: 7,563
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 53 of 173
            Pseudo Genes (incomplete)         :: 140 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 28 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:21:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 0
            Orig. end                         :: 18542
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSI01000022.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..18542
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="terpene"
                     /region_number="1"
                     /rules="(PT_phytoene_like or phytoene_synt or Lycopene_cycl
                     or Lycopene_cycl_fung or T1TS or T1TS_KS or T2TS or TS_UbiA
                     or TS_Pyr4)"
                     /tool="antismash"
     cand_cluster    1..18542
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /kind="single"
                     /product="terpene"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..18542
                     /aStool="rule-based-clusters"
                     /category="terpene"
                     /contig_edge="True"
                     /core_location="[1835:8542](-)"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      complement(1836..8542)
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(PT_phytoene_like or phytoene_synt or
                     Lycopene_cycl or Lycopene_cycl_fung or T1TS or T1TS_KS or
                     T2TS or TS_UbiA or TS_Pyr4)"
                     /neighbourhood="10000"
                     /product="terpene"
                     /protocluster_number="1"
     gene            complement(<1..162)
                     /locus_tag="IE189_RS36295"
     CDS             complement(<1..162)
                     /EC_number="1.17.7.3"
                     /GO_function="GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA]"
                     /GO_process="GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011031167.1"
                     /locus_tag="IE189_RS36295"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="WP_368858671.1"
                     /transl_table=11
                     /translation="MSAVALGLPGMPVRPVAARRVSRRIQVGSVAVGGDAPVSVQSMTT
                     TRTSDIGAT"
     gene            complement(167..1189)
                     /gene="hpnH"
                     /locus_tag="IE189_RS36300"
                     /old_locus_tag="GCM10010496_73860"
     CDS             complement(167..1189)
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_function="GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PF04055"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) QueE"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386169.1"
                     /locus_tag="IE189_RS36300"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_73860"
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="WP_189928046.1"
                     /sec_met_domain="PF04055 (E-value: 4.6e-19, bitscore: 61.9,
                     seeds: 518, tool: rule-based-clusters)"
                     /sec_met_domain="QueE (E-value: 2.6e-10, bitscore: 32.6,
                     seeds: 32, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAMPLRQSIKVATYLAEQKLRKRDKFPLIVELEPLFACNLKCEGC
                     GKIQHPAGVLKQRMPVAQAVGAVLESGAPMVSIAGGEPLMHPQMDEIVRQLVAKRKYVF
                     LCTNAMLLRKKMDKFTPSPYFAFAVHIDGLRERHDESVAKEGVFDEAVAAIKEAKRRGF
                     RVTTNSTFFNTDTPQTIIEVLNFLNDDLKVDEMMISPAYAYEKAPDQEHFLGVEQTREL
                     FKKAFAGGNRRRWRLNHSPLFLDFLEGKVDFPCTAWAIPNYSLFGWQRPCYLMSDGYVP
                     TYRELIEETDWDKYGRGKDPRCANCMAHCGYEPTAVLATMGSLKESLRAMRETVNGNRE
                     "
     misc_feature    1179..1192
                     /note="TFBS match to ArgR, Regulator of arginine
                     biosynthesis genes, confidence: weak, score: 16.85"
                     /tool="antismash"
     gene            complement(1195..1836)
                     /locus_tag="IE189_RS36305"
                     /old_locus_tag="GCM10010496_73870"
     CDS             complement(1195..1836)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016431357.1"
                     /locus_tag="IE189_RS36305"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_73870"
                     /product="1-hydroxy-2-methyl-2-butenyl 4-diphosphate
                     reductase"
                     /protein_id="WP_189928047.1"
                     /transl_table=11
                     /translation="MSTQPVPDPLLIACALGIEHLALRMGDRGGADGPVTVLRTGMGPR
                     AAERSVTRILADPVFAGAAVLATGFCAGLAPGMHPGDLVVAEETRDPRGAVPCVGTELL
                     VKELARTLPGRTVHTGPLTGSDHVVRGHERADLLATGAIAVDMESAATLLSAVRGGVRP
                     VAAVRVVVDAPEHELVRIGTVRGGISAFRVLRSVLPAFFEWHRNVLLPRR"
     gene            complement(1836..3857)
                     /gene="shc"
                     /locus_tag="IE189_RS36310"
                     /old_locus_tag="GCM10010496_73880"
     CDS             complement(1836..3857)
                     /EC_number="5.4.99.17"
                     /GO_function="GO:0016866 - intramolecular transferase
                     activity [Evidence IEA]"
                     /GO_function="GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA]"
                     /GO_process="GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="shc"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: T2TS"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129470.1"
                     /locus_tag="IE189_RS36310"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_73880"
                     /product="squalene--hopene cyclase"
                     /protein_id="WP_189928048.1"
                     /sec_met_domain="T2TS (E-value: 8.1e-150, bitscore: 492.3,
                     seeds: 58, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTATTDGSTGALPPRAAAASETDITIPVAAGVQEAAAHAMQRATD
                     FLLAGQDAEGWWKGDLETNVTMDAEDLLLRQFLGIRDEATTRAAALFVRGEQREDGTWA
                     TFHGGPPDLSATVEAYVALRLAGDEPGAPHMLRASAWVREQGGIASARVFTRIWLALFG
                     WWKWEDLPELPPELLFFPKWFPLSIYQFGCWARQTIVPLTVVSAKRPVRPAPFPLDELH
                     TDPARPNPPKPLAPVNSWDGLFQRLDKVVRGYRSVAVRRLRRAALNSAARWIIERQEND
                     GCWGGIQPPAVYSLIALHLLGYDLAHPVMRAGLESLDRYTVWREDGARMVEACQSPVWD
                     TCLATIALADAGLPADHPQLVKAADWMLGEQIVRPGDWSVRRPQLPPGGWAFEFHNDNY
                     PDIDDTAEVVLALRRVKHHDQERMDRAVARGVRWNLGMQSRNGAWGAFDVDNTSPFPNR
                     LPFCDFGEVIDPPSADVTAHVVEMLAIEGLSHDPRTRRGIEWLLTEQEPDGSWFGRWGV
                     NYVYGTGSVVPALTAAGLSVRHPAIRRAVGWLERVQNEDGGWGEDLRSYRDAARWSARG
                     ASTASQTAWALMALLAAGEKDSEAVARGVAWLARTQREDGSWDEPYFTGTGFPWDFSIN
                     YHLYRQVFPLTALGRYVHGEPFGQQSAPHAAHTEAEGS"
     gene            complement(3975..5003)
                     /locus_tag="IE189_RS36315"
                     /old_locus_tag="GCM10010496_73890"
     CDS             complement(3975..5003)
                     /GO_process="GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_FPPS_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1182: Polyprenyl synthetase"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129469.1"
                     /locus_tag="IE189_RS36315"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_73890"
                     /product="polyprenyl synthetase family protein"
                     /protein_id="WP_189928182.1"
                     /sec_met_domain="PT_FPPS_like (E-value: 3.7e-58, bitscore:
                     189.3, seeds: 145, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDVTALLERGRTLATPVLRAAVDRLAPPMDTVAAYHFGWIDAQGN
                     PTDGDGGKAVRPALAVLSAEVTGAAPEVGVPGAVAVELVHNFSLLHDDLMDGDEQRRHR
                     DTVWKVHGPAQAILVGDALFALAGEILLELGTVEAARATHRMTVATRALIDGQAQDISY
                     EHRDRVSVEECLEMEGNKTGALLACASSIGAVLGGADARTADALEKYGYHLGLAFQAVD
                     DLLGIWGDPEATGKQTWSDLRQRKKSLPVVAALAAGGSASERLGEILAADAKSSEFDSF
                     SEGEFAARAALIEEAGGRDWTAEEARRQHTVALEALNAVDMPEQVRDRFTALADFVVVR
                     KR"
     gene            complement(5114..6535)
                     /gene="hpnE"
                     /locus_tag="IE189_RS36320"
                     /old_locus_tag="GCM10010496_73900"
     CDS             complement(5114..6535)
                     /EC_number="1.17.8.1"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnE"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DAO"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1222: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129468.1"
                     /locus_tag="IE189_RS36320"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_73900"
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="WP_189928049.1"
                     /sec_met_domain="DAO (E-value: 3.4e-12, bitscore: 39.0,
                     seeds: 521, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDEQHADAAGASGPPARGAVVVGGGLAGVTAALALADAGVRVTL
                     LEGRPRLGGLAFSFRRGDLTVDNGQHVYLRCCTAYRWFLDRIEGSALAPLQDRLDVPVV
                     DLARPAGRRLGRLRRDALPVPLHLGRSLATYPHLSLAERAAVGRAALALKGLDLTDPAL
                     DTQDFGSWLTAHGQSPRAVEALWDLVGVATLNAVAGDSSLALAAMVFKTGLLSDPGAAD
                     IGWAHVPLGDLHDGLARKALDSAGVRTEVRTRVTSLSRDGRGTWSVQVPGETLHADAVV
                     LAVPQREAYDLLPDGALDDAGRLLDIGTAPILNVHVVYDRKVLDKPFFAALGTPVQWVF
                     DRTAASGLREGQYLALSQSAAQDEIDEPVAALRRRYLPELERLLPRTRGAEVRDFFVTR
                     ERTATFAPGPGVGRLRPGARTRAPGLYLAGAWTATGWPATMESAVRSGVGAADAALGTL
                     GRPRPRHLFAFEEAA"
     gene            complement(6532..6684)
                     /locus_tag="IE189_RS39870"
                     /old_locus_tag="GCM10010496_73910"
     CDS             complement(6532..6684)
                     /codon_start=1
                     /inference="COORDINATES: protein motif:HMM:NF039420.3"
                     /locus_tag="IE189_RS39870"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_73910"
                     /product="DUF6380 family protein"
                     /protein_id="WP_351008924.1"
                     /transl_table=11
                     /translation="MDTAPDTEGRGDRTGGKRHATLRSGAASLTATTGGAEFDHHGRSA
                     REGAG"
     gene            complement(6684..7634)
                     /gene="hpnD"
                     /locus_tag="IE189_RS36325"
                     /old_locus_tag="GCM10010496_73920"
     CDS             complement(6684..7634)
                     /EC_number="2.5.1.103"
                     /GO_function="GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnD"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129467.1"
                     /locus_tag="IE189_RS36325"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_73920"
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="WP_189928050.1"
                     /sec_met_domain="phytoene_synt (E-value: 3.4e-59, bitscore:
                     192.8, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PT_phytoene_like (E-value: 1.3e-70,
                     bitscore: 230.5, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRAVEPSPHVSAPVLAAYGYCETVTGQQARNFAYGIRLLPTSKR
                     RAMSALYAFSRRVDDIGDGALDHEVKTARLEDTRALLARVRAGEVEEDDTDPVAVALAH
                     TARTFPVPLRGLDELIDGVLMDVRGETYETWDDLKVYCRCVAGAIGRLSLGVFGTAPGA
                     RGAERAPEYADTLGLALQLTNILRDVREDAEGGRTYLPADDLAKFGCSAGFDGPKPPEG
                     SDFVGLVHFEVRRARALFAEGYRLLPMLDRRSGACVAAMAGIYRRLLDRIERDPEAVLR
                     GRVSLPGREKAYVAVRGLSGLDTRHVSRRTVRRNP"
     gene            complement(7631..8542)
                     /gene="hpnC"
                     /locus_tag="IE189_RS36330"
                     /old_locus_tag="GCM10010496_73930"
     CDS             complement(7631..8542)
                     /EC_number="2.5.1.21"
                     /GO_function="GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /gene="hpnC"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: phytoene_synt"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     terpene: PT_phytoene_like"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129466.1"
                     /locus_tag="IE189_RS36330"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_73930"
                     /product="squalene synthase HpnC"
                     /protein_id="WP_189928051.1"
                     /sec_met_domain="phytoene_synt (E-value: 2.3e-37, bitscore:
                     121.2, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PT_phytoene_like (E-value: 2.9e-40,
                     bitscore: 130.8, seeds: 61, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTAPAVPRVGDPERGTLDKAADENFPVAPFFLPKAWRADLMAVYG
                     FARLVDDIGDGDLAPGGADARLLGVPAADAHDRLLMLDAFEADLRRVFDGSDGPPRHPL
                     LRRLQPTVRARALTPGPFLGLIAANRQDQLVKRYETYDDLLAYCELSANPVGRLVLAVT
                     GAATPERIRRSDAICTALQIVEHLQDVAEDLGRDRVYLPAEDMKRFHVREADLAAKTAG
                     ASVRALVAYEAQRAGDLLNEGAPLVGSVHGRLRLLLAGFVAGGRAAITAITAAGHDVLP
                     GPPKPGRFQLLREVGVTLRGKG"
     gene            complement(8901..9689)
                     /locus_tag="IE189_RS36335"
                     /old_locus_tag="GCM10010496_73940"
     CDS             complement(8901..9689)
                     /codon_start=1
                     /gene_functions="transport (smcogs) SMCOG1000: ABC
                     transporter ATP-binding protein"
                     /gene_kind="transport"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020129465.1"
                     /locus_tag="IE189_RS36335"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_73940"
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="WP_189928052.1"
                     /transl_table=11
                     /translation="MVEPLPGQRVPTVIADEVHIVYRVNGAKSGKGSATAALSRIVKRG
                     SADSARGVRKVHAVRGVSFVAHRGEAIGLIGSNGSGKSTLLRAIAGLLPPESGRVYTDG
                     QPSLLGVNAALMNDLTGERNVTLGGLAMGMSREQIKQRFQEIVDFSGINEKGDFITLPM
                     RTYSSGMQARLRFSIAAAKDHDVLMIDEALATGDAKFRGRSEERIRQLREHAGTVFLVS
                     HSNKSIRDTCDRVLWLERGELRLDGPTEEVMREYEKFSGK"
     gene            complement(9682..10611)
                     /locus_tag="IE189_RS36340"
                     /old_locus_tag="GCM10010496_73950"
     CDS             complement(9682..10611)
                     /GO_component="GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /GO_function="GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994380.1"
                     /locus_tag="IE189_RS36340"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_73950"
                     /product="ABC transporter permease"
                     /protein_id="WP_189928054.1"
                     /transl_table=11
                     /translation="MSETTHDVGVVVTASPSPDEGLTAAQLAARYGLAVSGARPPLREY
                     VRQLWGRRHFIMAFSRAKLASQYSQARLGQLWQVATPLLNAAVYFMIFGLLLNAGRGMS
                     KEVYIPFLVTGVFVFTFTQSSVMTGVRAISGNLGLVRALHFPRASLPISFALQQLQQLL
                     YSMLVLFLVVIGFGSYPDASWALIVPVLALQFCFNSGLALIFARAGAKTPDLAQLMPFV
                     MRTWMYASGVMFSIPVFLADKPQWVANILQWNPAAIYMDLMRFALIEEYAAENLPAHVW
                     AVAGGWAVVVALGGFVYFWQAEERYGRG"
     gene            complement(10712..11596)
                     /locus_tag="IE189_RS36345"
                     /old_locus_tag="GCM10010496_73960"
     CDS             complement(10712..11596)
                     /EC_number="2.4.-.-"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983096.1"
                     /locus_tag="IE189_RS36345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_73960"
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_189928056.1"
                     /sec_met_domain="Glycos_transf_2 (E-value: 9.7e-13,
                     bitscore: 40.8, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSAPVKVGAVVITMGNRPEELRALLDSIAGQDGDRVEAVVVGNGS
                     PVPDVPEGVRTIELPENLGIPGGRNVGIEAFGPSGRDVDILLFLDDDGLLARKDTAELC
                     REAFGADPRLGIVSFRIADPQTGVTQRRHVPRLRASDPMRSSRVTTFLGGANAVRTKVF
                     AEVGGLPDEFFYAHEETDLAWRALDAGWMIDYRSDMVLYHPTTAPSRHAVYHRMVARNR
                     VWLARRNLPAPLVPVYLGVWLLLTLARRPSGSALKAWFGGFREGWSAPCGPRRPMKWRT
                     VWRLTRLGRPPVI"
     gene            complement(11593..12372)
                     /locus_tag="IE189_RS36350"
                     /old_locus_tag="GCM10010496_73970"
     CDS             complement(11593..12372)
                     /EC_number="2.7.8.-"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /GO_function="GO:0000287 - magnesium ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /GO_process="GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014049651.1"
                     /locus_tag="IE189_RS36350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_73970"
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="WP_189928058.1"
                     /transl_table=11
                     /translation="MSRPSVAELRPVVHPPGVKDRRSGEHWMGRLYMREVSLRVDRYLV
                     NTRVTPNQLTYLMTVFGVLAAPALLVPGITGAVLGVVCVQMYLLLDCVDGEIARWKKQY
                     SLGGVYLDRVGAYLTDAAVLVGFGLRAADPWGSGRIDWLWAFLGTLAALGAILIKAETD
                     LVGVARHQGGLPPVKESASEPRSSGMALARRAASALQFHRLILGIEASLLILVLAVVDQ
                     VRGDLFFSRLGVAVLAGIALLQTLLHLVSILASSRLK"
     gene            complement(12350..13411)
                     /locus_tag="IE189_RS36355"
                     /old_locus_tag="GCM10010496_73980"
     CDS             complement(12350..13411)
                     /GO_function="GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fe-ADH"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) DHQ_synthase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1181: dehydrogenase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007494151.1"
                     /locus_tag="IE189_RS36355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_73980"
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="WP_189928060.1"
                     /sec_met_domain="Fe-ADH (E-value: 8.3e-26, bitscore: 83.1,
                     seeds: 218, tool: rule-based-clusters)"
                     /sec_met_domain="DHQ_synthase (E-value: 1.9e-10, bitscore:
                     32.8, seeds: 76, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPVLTRLIPSPVVVDIRPGALDDLVGVLADERISHSGKLALAVSG
                     GSGARLRERLTPALPGATWYEVGGGTLDDAVRLAADIKAGHFDAVVGLGGGKIIDCAKF
                     AAARVGLPLVAVPTNLAHDGLCSPVATLDNDAGRGSYGVPNPIAVVIDLDVIREAPVRY
                     VRAGIGDAVSNISAIADWELANRVKGEKIDGLAAAIARQAGEAVLRHPGSIGDTDFLQV
                     LAEALVLSGIAMSVSGDSRPSSGACHEINHAFDLLFPKRAAAHGEQCGLGAAFAMYLRG
                     AHEESAYMAQVLRRHGLPVLPTDIGFTVDEFVRAVEFAPETRPGRYTIIEHLDLKTEQI
                     KDIYADYVKAIGS"
     gene            complement(13399..14151)
                     /locus_tag="IE189_RS36360"
                     /old_locus_tag="GCM10010496_73990"
     CDS             complement(13399..14151)
                     /GO_function="GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064: glucose-1-phosphate
                     adenylyl/thymidylyltransferase"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983099.1"
                     /locus_tag="IE189_RS36360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_73990"
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="WP_189928061.1"
                     /sec_met_domain="NTP_transf_3 (E-value: 1.1e-11, bitscore:
                     37.8, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIGLVLAAGAGRRLRPYTDSLPKALVPVGPAGIEGEPTVLDLTLG
                     NFAEIGLTEVAVIVGYRKEAVYARKAALEAKYGLKLTLIDNDKAEEWNNAYSLWCGRDA
                     LKDGVILANGDTVHPVSVEKTLLAARGEGRRIILALDTVKSLADEEMKVVVDPAKGMTK
                     ITKLMDPAEATGEYIGVTLIEGDAAPELADALRTVWETDPQQFYEHGYQELVNRGFRID
                     VAPIGDVEWVEIDNHDDLARGREIACQY"
     gene            complement(14148..16001)
                     /locus_tag="IE189_RS36365"
                     /old_locus_tag="GCM10010496_74000"
     CDS             complement(14148..16001)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003996269.1"
                     /locus_tag="IE189_RS36365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_74000"
                     /product="DUF5941 domain-containing protein"
                     /protein_id="WP_189928062.1"
                     /transl_table=11
                     /translation="MPTAILTGSPVPGSSIEGDLRSLGFDVRTASDAGEAEALLAAVPG
                     DLRVAVVDARFVGHEHALRLGLTDPRFPIAAIPGAVTAQPAGRQALTRAIARENSASGS
                     AAPGSAAHGSAPARAAAGVEDVDSLADRIVTALDADGADVHRPELGSLVAEVPADPQAR
                     NEARQAVAAVDDEAVRLKSAVKARDGFFTTHFISPYSRYLARWCARRGLTPNQVTTASL
                     ITALIAAACAATGTRGGFVAAGVLLIASFVLDCTDGQLARYSLQYSTLGAWLDATFDRA
                     KEYAYYAGLALGAARGGDDVWALALGAMVLQTCRHVVDFSFNEANHDATANTSPTAALS
                     DKLDSVGWTVWVRRMIVLPIGERWAMIAVLTAATTPRITFYALLVGCAFAATYTTAGRV
                     LRSLTRKARRTDRAAQALADLADSGPLTELLARRLPAGIRIPAPLGASAGVVLLVLAAA
                     FDGPGFSLLLAAVLYVLLCADAVARPLKGALDWLVPPLFRAGEYCTVLILAAKAGADGA
                     LPAAFGLVAAVAYHHYDTVYRIRGDAGAPPAWLVRAIGGQDGRTLLVAVLAVALTASQF
                     TVALTVLAVAVAVVVLVESIRFWVAAHRAGAPSVHDEGEPA"
     misc_feature    16221..16235
                     /note="TFBS match to MexT, Global virulence regulator,
                     confidence: weak, score: 16.81"
                     /tool="antismash"
     misc_feature    complement(16727..16741)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: weak, score: 18.72"
                     /tool="antismash"
     gene            16835..17776
                     /locus_tag="IE189_RS36370"
                     /old_locus_tag="GCM10010496_74010"
     CDS             16835..17776
                     /GO_function="GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339288.1"
                     /locus_tag="IE189_RS36370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_74010"
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="WP_189928063.1"
                     /transl_table=11
                     /translation="MGAGHDHGHTHAHVPASGTAAAAYRGRLRTALSITLGVMVVEIVG
                     GVLADSLALVADAAHMATDALGLGMALLAIHFANRPPSENRTFGLARAEILAALANCLL
                     LLGVGGYVLYEAVQRFFTPAATEGGLMIWFGAIGLVANMVSLSLLMRGQAESLNVRAAF
                     LEVAADALGSVAVLISAVVILTTGWEAADPIASLVIGLMIVPRTVKLLRETLDVLLESA
                     PKDVDMAEVRSHVLALDGVEDIHDLHAWTITSGMPVLSAHVVVRSDVLNAIGHEKMLHE
                     LQGCLGDHFDVEHCTFQLEPIGHAEHEARLCH"
ORIGIN
        1 cgtggcaccg atgtccgacg tacgcgtcgt cgtcatcgac tgcaccgaca ccggggcatc
       61 cccaccgacc gcgacggacc ccacctggat ccgccgcgac acgcggcgcg ccgccaccgg
      121 ccggaccggc atcccgggca ggcccagggc gacggcgctc acgcggtcac tcccggttcc
      181 cgttgacggt ctcgcgcatg gcgcgcaggg actccttcag ggaacccatg gtggcgagga
      241 cggcggtcgg ctcgtagccg cagtgcgcca tgcagttggc gcagcgcggg tccttgcccc
      301 ggccgtactt gtcccagtcc gtctcctcga tcagctcgcg gtacgtgggc acgtacccgt
      361 cgctcatcag gtagcagggc cgctgccagc cgaagagcga gtagttgggg atggcccacg
      421 cggtgcacgg gaagtcgacc ttgccctcga ggaagtcgag gaacagcggc gagtggttga
      481 gccgccagcg gcgccggttg ccgcccgcga aggccttctt gaacagttcc cgggtctgct
      541 ccacaccgag gaagtgctcc tggtcggggg ccttctcgta ggcgtaggcg ggcgagatca
      601 tcatctcgtc gaccttgagg tcgtcgttga ggaagttgag cacctcgatg atggtctgcg
      661 gggtgtcggt gttgaagaac gtcgagttgg tggtcacccg gaagccgcgc cgcttggcct
      721 ccttgatggc cgccacggcc tcgtcgaaca cgccctcctt ggccaccgac tcgtcgtgcc
      781 gctcccgcag cccgtcgatg tgcacggcga aggcgaagta cggggagggg gtgaacttgt
      841 ccatcttctt gcgcagcagc atggcgttgg tgcagaggaa gacgtacttt ctcttcgcca
      901 ccaactgccg cacgatctcg tccatctgag gatgcatcag gggctcgccg cccgcgatgg
      961 acaccatcgg cgcaccggac tccagcaccg cgccgacggc ctgggcgacc ggcatgcgct
     1021 gcttgagcac tccggccgga tgctggatct tgccgcagcc ctcgcacttg aggttgcagg
     1081 cgaagagggg ctccagctcc acgatgagcg ggaacttgtc ccgcttgcgg agcttctgtt
     1141 cagccaggta tgtagcgacc ttgatggact gacgcaacgg catggccatg cggctcacct
     1201 ccgggggagc agaacattac ggtgccattc gaaaaaagca ggcaggaccg agcggagaac
     1261 gcggaaagcc gatattccac cgcgtaccgt gccgatccgg acgagttcat gttcaggagc
     1321 gtccacgacc acccggacgg ccgcaaccgg gcgtactccc ccgcgcacgg cgctcaggag
     1381 cgtggccgcc gactccatgt cgacggcgat cgcgccggtc gccagcaggt cggcccgctc
     1441 gtgaccgcgt acgacgtggt cggagccggt gagcgggccg gtgtggacgg tgcgcccggg
     1501 cagggtgcgc gcgagttcct tgacgagcag ttcggtgccg acgcagggga cggcgccgcg
     1561 cgggtcgcgg gtctcctcgg cgacgaccag gtcgccgggg tgcatgcccg gggcgagccc
     1621 cgcgcagaat ccggtggcca gcacggccgc ccccgcgaac accgggtcgg ccaggatccg
     1681 ggtgacggaa cgctccgcag ccctcgggcc catgcccgtc cgcaggacgg tgacgggacc
     1741 gtcggctccg ccgcggtcgc ccatgcgcag ggcgaggtgc tcgatgccga gggcgcaggc
     1801 gatcagcagc gggtccggga cgggctgcgt gctcatcagc tcccctctgc ctcggtgtgg
     1861 gccgcgtgcg gggcggactg ctggccgaac ggttctccgt ggacgtaccg gccgagggcg
     1921 gtcagcggga agacctgccg gtagaggtgg tagttgatcg agaagtccca cgggaagccg
     1981 gtgccggtga agtacggctc gtcccaggag ccgtcctccc gctgggtccg cgccagccag
     2041 gccacaccgc gcgcgacggc ctcggagtcc ttctcgccgg ccgccaggag cgccatcagc
     2101 gcccacgcgg tctgtgaggc ggtcgaggcg ccccgggcgc tccagcgggc ggcgtcccgg
     2161 taggagcgca ggtcctcgcc ccagccgccg tcctcgttct gcacccgctc cagccagccg
     2221 accgcccgcc tgatcgcggg gtgccggacg gacaggccgg ccgcggtgag ggcgggcacc
     2281 accgaccccg tgccgtagac gtagttgacg ccccagcgcc cgaaccacga gccgtccggc
     2341 tcctgttcgg tcagcagcca ctcgatgccg cgccgggtgc gcgggtcgtg cgagagcccc
     2401 tcgatcgcca gcatctccac gacgtgggcg gtgacatcgg ccgacggcgg gtcgatgacc
     2461 tcgccgaagt cgcagaacgg caaccggttg gggaacgggc tggtgttgtc gacgtcgaag
     2521 gctccccagg ccccgttcct cgactgcatc ccgaggttcc agcgcacgcc gcgcgcgacg
     2581 gcccggtcca tgcgttcctg gtcgtggtgc ttgacccggc gcagggcgag gacgacctcc
     2641 gcggtgtcgt cgatgtcggg gtagttgtcg ttgtggaact cgaaggccca tcctcccggc
     2701 gggagttggg gccggcgcac cgaccagtcg ccgggccgga cgatctgctc cccgagcatc
     2761 cagtcggcgg ccttgaccag ttgcgggtgg tcggcgggaa gtccggcgtc ggcgagcgcg
     2821 atggtcgcca gacaggtgtc ccacaccggc gactggcagg cctcgaccat ccgcgcgccg
     2881 tcctcgcgcc acacggtgta gcggtccagc gactccagtc cggcgcgcat cacggggtgg
     2941 gcgaggtcgt agccgagcag gtgaagggcg atcagcgagt acacggcggg cggctggatc
     3001 ccgccccagc agccgtcgtt ctcctgtcgc tcgatgatcc agcgggcggc ggagttcagg
     3061 gccgcccggc gcagccggcg caccgcgacc gaccggtagc cgcgcaccac cttgtcgagc
     3121 cgctggaaga gcccgtccca actgttgaca ggtgccaggg gtttgggggg attgggccgg
     3181 gcggggtcgg tgtgcagttc gtccagcggg aacggcgccg gacgcaccgg gcgcttcgcc
     3241 gacacgacgg tgaggggcac gatcgtctgc cgcgcccagc agccgaactg gtaaatgctc
     3301 aggggaaacc atttagggaa gaacaacagc tcagggggga gttcggggag gtcctcccac
     3361 ttccaccagc cgaacagggc cagccagatc cgggtgaaga cccgggcgga cgcgataccg
     3421 ccctgctcgc ggacccacgc ggaggcccgg agcatgtggg gcgccccggg ctcgtcgccg
     3481 gcgagtcgca gcgcgacata ggcctcgacg gtcgcggaca ggtcgggcgg gccgccgtgg
     3541 aaggtggccc aggtgccgtc ctcgcgttgc tcgccgcgga cgaagagggc ggcggcccgg
     3601 gtggtggcct cgtcgcggat accgaggaac tgacggagca gcaggtcctc ggcgtccatc
     3661 gtgacgttgg tctcgaggtc gcccttccac cacccctcgg cgtcctgccc ggcgagcagg
     3721 aagtcggtgg cgcgttgcat cgcgtgcgcg gcggcttctt gtaccccggc cgccacgggg
     3781 atggtgatgt cggtttcgct ggccgcggca gcgcggggcg ggagagcccc ggtgcttccg
     3841 tcggtcgtcg ctgtcatggc ttccccttcg tgcagtgtgc atggcgttgc gtctgctgtg
     3901 ggtccgccgt cggccggtgc cgtctcctcg cacaccggcc ggcgactacg cgagggctat
     3961 tcgaccgata gggatcatct cttccgtacg acgacgaagt cggcgagcgc cgtgaaccgg
     4021 tcccgcacct gctcgggcat gtccacggcg ttcagggcct ccagggcgac cgtgtgctga
     4081 cggcgtgcct cctcggcggt ccagtcacgg ccgcccgcct cctcgatgag cgcggcgcgt
     4141 gcggcgaact cgccttccga gaagctgtcg aactcgctgc tcttggcgtc ggcggcgagg
     4201 atctccccga gccgctcgga ggcggatccg cccgccgcga gcgcggccac caccggcagg
     4261 gacttcttgc gctggcgcag gtcgctccag gtctgcttgc cggtggcctc gggatcgccc
     4321 cagatgccga ggaggtcgtc gacggcctgg aaggccaggc cgaggtggta gccgtacttc
     4381 tccagggcgt cggcggtgcg ggcgtccgcg ccgccgagca cggcgccgat ggagctggcg
     4441 caggcgagca gggcgccggt cttgttgccc tccatctcca ggcactcctc gacgctgacc
     4501 cggtcgcggt gctcgtagga gatgtcctgg gcctggccgt cgatgagggc ccgggtggcg
     4561 acggtcatcc ggtgggtggc ccgggccgcc tcgaccgtgc cgagttcgag gaggatctcg
     4621 ccggcgaggg cgaacagggc gtcgccgacc aggatggcct gggcgggacc gtgcaccttc
     4681 cagacggtgt cgcggtgcct gcgctgctcg tcgccgtcca tcaggtcgtc gtgcaggagc
     4741 gagaagttgt ggaccagttc gaccgcgacc gcgccgggga cgccgacctc gggggcggcc
     4801 ccggtgacct cggcggacag caccgcgagg gcgggacgca cggccttgcc gccgtcgccg
     4861 tcggtcgggt tgccctgggc gtcgatccag ccgaagtggt aggcggcgac cgtgtccatg
     4921 ggcggtgcca gacggtcgac ggccgcccgc agcaccggtg tggccagggt gcggccgcgc
     4981 tccaggagcg cggtcacatc caccgcggtc cgccgagcgg ccggcgtggc cgggggcaca
     5041 gtgggcactg tttctcctct tgttgcggtg ccggagaggg tgcgggggtg tgccgagccg
     5101 tgctgatcga gcatcaggcc gcctcctcga acgcgaagag gtggcggggg cggggccggc
     5161 ccagcgtgcc gagcgcggcg tccgccgcgc cgacaccgct gcggaccgca ctctccatgg
     5221 tcgcgggcca ccctgtggcg gtccacgctc cggccaggta caggccgggg gctctggtgc
     5281 gggcgccggg ccgaagccgc ccgacgccgg gcccgggggc gaacgtcgcc gtacgctccc
     5341 gggtcacgaa gaagtccctc acctcggcgc cgcgcgtacg gggcagcagc ctctccagtt
     5401 cgggcaggta gcgccggcgc agggcggcga cgggttcgtc gatctcgtcc tgcgcggcgg
     5461 actgggacag ggcgaggtac tggccctcgc gcagcccgga ggcggcggtg cggtcgaaga
     5521 cccactgcac gggcgtgccg agcgcggcga agaagggctt gtcgagcacc ttgcggtcgt
     5581 agacgacgtg cacgttgagg atgggcgcgg tgccgatgtc cagcagccgc ccggcgtcgt
     5641 ccagcgcccc gtcggggagc aggtcgtagg cctcgcgctg cggtacggcg aggacgaccg
     5701 cgtccgcgtg gagcgtctcg ccggggacct ggacgctcca ggtcccccgg ccgtcgcggg
     5761 agagcgaggt gacgcgggtg cggacctcgg tccgcacgcc agcggagtcg agggccttgc
     5821 gggcgagccc gtcgtgcagg tcgcccagcg ggacgtgcgc ccagccgatg tcggcggcgc
     5881 ccgggtcgga cagcagaccg gtcttgaaca ccatggcggc gagcgccagg gaggagtcgc
     5941 ccgcgaccgc gttcagggtg gcgaccccga ccaggtccca gagggcctcg acggcgcgcg
     6001 gcgactggcc gtgcgcggtc agccagctgc cgaagtcctg ggtgtcgagt gccggatcgg
     6061 tgaggtccag ccccttgagc gcgagggcgg cccggccgac ggcggcgcgt tcggcgaggg
     6121 acaggtgcgg atacgtcgcg aggctgcgcc cgaggtgcag ggggacgggc agcgcgtcgc
     6181 gccgcagtcg gccgagccgc cgcccggcag gtctggccag gtcgacgacg ggcacgtcga
     6241 gacggtcctg gagcggtgcc agcgcgctgc cctcgatgcg gtcgaggaac cagcggtacg
     6301 cggtgcagca gcgcaggtag acgtgctgtc cgttgtccac ggtgaggtcg ccgcgccgga
     6361 aggagaaggc gaggccgccg agccggggcc ggccttcgag cagggtgacg cgcactcccg
     6421 cgtcggcgag ggcgagcgcg gcggtcaccc cggcgagtcc gccgcccacg acgacggcgc
     6481 cgcgcgccgg gggcccggac gcgccggcgg cgtccgcgtg ctgctcgtcg ctcatcctgc
     6541 gccctcccgt gcggaccgtc cgtggtggtc gaactctgca ccgccggtcg tcgcagtcag
     6601 ggacgccgcg ccggaccgga gggttgcgtg ccgttttccg ccggtgcggt cgccccggcc
     6661 ctcggtgtcc ggcgccgtgt ccatcagggg ttcctcctga cggtgcggcg ggacacgtgc
     6721 cgggtgtcga ggcccgacag cccgcgcacg gccacgtacg ccttctcgcg tccggggagc
     6781 gagacccggc cgcgcaggac ggcctcgggg tcgcgctcga tgcggtccag gaggcgccgg
     6841 tagatgccgg ccatcgcggc gacgcaggcg ccgctgcggc ggtcgagcat gggcagcagc
     6901 cggtagccct cggcgaagag ggcccgggcc cggcgcacct cgaagtggac caggcccacg
     6961 aagtcggacc cctccggcgg cttcgggccg tcgaacccgg ccgagcagcc gaacttcgcg
     7021 aggtcgtcgg cgggcagata ggtacggccg ccctcggcgt cctcgcggac gtccctgagg
     7081 atgttggtga gctggagcgc gagcccgagc gtgtcggcgt actccggcgc gcgctcggcg
     7141 ccgcgcgccc cgggcgcggt gccgaagacg ccgagcgaga gccgaccgat ggcgcccgcc
     7201 acgcagcggc agtacacctt caggtcgtcc caggtctcgt acgtctcgcc gcgtacgtcc
     7261 atcaggacgc cgtcgatcag ctcgtccagg ccgcgcagcg gcacggggaa ggtgcgggcg
     7321 gtgtgcgcga gggcgaccgc gacggggtcg gtgtcgtcct cctcgacctc gccggcgcgg
     7381 acgcgggcga gcagcgccct ggtgtcctcg agccgggcgg tcttgacctc gtggtccagg
     7441 gcgccgtcgc cgatgtcgtc gacgcggcgc gagaaggcgt acagcgcgga catcgcgcgg
     7501 cgcttggacg tgggcagcag gcggatgccg tacgcgaagt tacgggcctg ctggccggtg
     7561 acggtctcgc agtagccgta ggcggcgagt accggcgcgg acacgtgcgg tgacggctcc
     7621 acggcccgga tcaccccttt cctcgcaggg tcacgcccac ctcgcgcagc agctggaacc
     7681 tgccgggctt gggcgggccg ggaagtacgt cgtgtccggc ggcggtgatc gccgtgatcg
     7741 ccgcccttcc ccccgccacg aatcccgcga gcagcagcct cagcctgccg tgaacgctac
     7801 ccaccagggg ggcgccttca ttcaggaggt cgccggcgcg ttgcgcctcg tacgcgacca
     7861 gggcgcgtac cgacgcgccc gcggtcttcg cggccaggtc cgcttcccgg acgtggaaac
     7921 gtttcatgtc ctctgcgggc aggtagacac ggtcgcggcc gaggtcctcg gccacgtcct
     7981 gaaggtgttc gacgatctgg agtgcggtgc agatcgcgtc ggagcggcgg atccgctcgg
     8041 gggtggcggc gccggtgacg gcgaggacca ggcggcccac cgggttggcg gacagctcgc
     8101 agtaggcgag caggtcgtca taggtctcgt agcgcttgac gagctggtcc tgccggttgg
     8161 ccgcgatcag gccgaggaag ggcccggggg tgagcgcgcg ggcgcgcact gtgggctgga
     8221 ggcggcgcag caggggatgg cgcggcgggc cgtcggaccc gtcgaagacc cgccgcagat
     8281 cggcctcgaa ggcgtccagc atcaggaggc ggtcgtgcgc gtcggcggcc ggcacaccga
     8341 gcagccgggc gtcggcgccg ccgggggcga ggtcgccgtc gccgatgtcg tcgacgagcc
     8401 gggcgaagcc gtacaccgcc atcaggtcgg cgcgccaggc cttgggcagg aagaaggggg
     8461 ctacggggaa gttctcgtcc gcggccttgt cgagcgtgcc gcgctccgga tcgccgacgc
     8521 gtggcacggc gggtgccgtc atcgtcgtcc gcccggggcg gggacggcac ggcctgcgtc
     8581 gagctgggga gtttccgtag ccatcgccgt cacatctccc gttctacact gccgacctaa
     8641 tacacactat ttcggacacg ccgcccggac gctcgcgcgg gtcccggcgg tgggcgctga
     8701 gcgttatcac cccacttgac gcgtttcggg ggccggtcca gcttacgttg tacaacgctg
     8761 cacggttcgc gggggtgtcc gggacatcac aacaacacac cgattggcgt caagattcct
     8821 gggcctcacc ggagttgacg tttcctttgc agacgcaggg ccccgccgga cagttccggc
     8881 ggggccctgg ccgaatcgcc ctacttgccc gagaacttct cgtactcccg catgacctct
     8941 tcggtcgggc cgtccagccg cagttcgccc ctctccagcc acagcacgcg gtcgcaggtg
     9001 tccctgatcg acttgttgct gtggctcacc aggaacaccg tgccggcgtg ctcgcgcagc
     9061 tgccggatgc gttcctcgga gcgcccccgg aacttggcgt cgccggtggc gagggcctcg
     9121 tcgatcatca ggacgtcatg gtccttggcg gcggcgatgg agaaccgcag ccgggcctgc
     9181 atgccggagg agtaggtgcg catcggcagg gtgatgaagt cgcccttctc gttgatgccc
     9241 gagaagtcga cgatctcctg gaagcgctgc ttgatctgct cgcgggacat ccccatggcg
     9301 agcccgccca gggtgacgtt gcgctcgccg gtgaggtcgt tcatcagggc cgcgttgacg
     9361 cccagcagcg agggctggcc gtcggtgtag acccggccgc tctccggcgg cagcagcccg
     9421 gcgatggcgc gcagcagcgt ggacttgccc gagccgttgg agccgatcag gccgatggcc
     9481 tcgccgcggt gggcgacgaa ggagacaccg cgcacggcgt gcaccttgcg caccccgcgg
     9541 gccgagtcgg cggaaccgcg cttgacgata cggctcagcg cggcggtggc actgcccttg
     9601 ccggacttgg cgccgttgac gcggtagacg atgtggacct cgtcggcgat gaccgtgggg
     9661 acccgctgtc ccgggagcgg ctcaaccacg gccgtacctc tcctccgcct gccagaagta
     9721 cacgaatccg ccgagcgcca cgaccacggc ccagccgccc gcgaccgccc agacgtgcgc
     9781 cggcaggttc tcggcggcgt actcctcgat gagcgcgaag cgcatcaggt ccatgtagat
     9841 ggcggccggg ttccactgga ggatgttggc cacccactgc ggcttgtccg cgaggaagac
     9901 ggggatcgag aacatcacgc cggacgcgta catccaggtc cgcatgacga acggcatcag
     9961 ctgcgccagg tccggggtct tcgcgccggc ccgggcgaag atcagcgcca gaccggagtt
    10021 gaagcagaac tggagggcca gcaccgggac gatcagcgcc caggaggcgt ccgggtagct
    10081 gccgaaaccg atcaccacca ggaacagcac cagcatcgag tacagcagct gctggagttg
    10141 ctggagggcg aacgagatgg gcagcgaggc acgcgggaag tgcagggcgc gcaccaggcc
    10201 gaggttgccg gagatcgccc gcaccccggt catcaccgag ctctgggtga aggtgaacac
    10261 gaagacgccc gtgacgagga aggggatgta cacctccttc gacatccccc ggccggcgtt
    10321 cagcagcagg ccgaagatca tgaagtacac ggccgcgttc agcagcgggg tggccacctg
    10381 ccacagctgg ccgagcctgg cctggctgta ctgggaggcc agcttggccc gggagaacgc
    10441 catgatgaag tgccggcgcc cccagagctg gcggacgtac tcccgcagcg gaggccgggc
    10501 gccgctcacg gccagcccgt acctggcggc cagctgcgcc gccgtgaggc cctcgtcggg
    10561 cgacggagac gccgtcacca cgactccgac gtcatgcgtt gtctcactca caagtaggaa
    10621 ctttcgtctt cgggatgcgc agccttgacg aggccggcac gagccggggg gcccgccgga
    10681 aaggcccggg gtgccctcag gaacgagctt gtcagatcac cggaggccgg cccagtcggg
    10741 tcagccgcca caccgtacgc cacttcatgg gcctgcgagg cccgcacgga gcgctccagc
    10801 cctcccggaa acccccgaac cacgccttca gcgcggaccc ggaggggcgg cgcgccagcg
    10861 tgagcagcag ccagacgccg agatagaccg ggaccagcgg ggcggggagg ttgcgacggg
    10921 ccagccagac ccggttgcgg gcgaccatcc ggtggtacac cgcgtgccgc gagggcgcgg
    10981 tggtcgggtg gtacagcacc atgtcggacc ggtagtcgat catccagccc gcgtccaggg
    11041 cccgccacgc caggtcggtc tcctcgtggg cgtagaagaa ctcgtcgggg agtccgccca
    11101 cctcggcgaa gaccttcgtc cgtacggcgt tggcgccccc gaggaaggtg gtgacccggg
    11161 aggagcgcat cgggtcggag gcccgcagcc ggggcacgtg acggcgctgg gtgacaccgg
    11221 tctgcgggtc cgcgatccgg aagctgacga tgccgagccg ggggtcggcg ccgaaggcct
    11281 cgcggcacag ctcggccgtg tccttgcggg ccaggaggcc gtcgtcgtcc aggaagagca
    11341 ggatgtcgac gtcgcggccg ctgggcccga aggcctcgat gccgacgttg cggccgccgg
    11401 ggatgccgag gttctcgggc agctcgatcg tcctgacgcc ctcggggacg tccgggacgg
    11461 gcgagccgtt gccgacgacg accgcctcga cccggtcgcc gtcctgcccg gctatcgagt
    11521 cgagcagggc cctgagctcc tcgggacggt tgcccatcgt gatcacgacc gcgccgacct
    11581 tcaccggcgc gctcacttca gcctgctcga cgcgaggatg gacaccaggt gcagcagggt
    11641 ctggagcagc gcgatgccgg ccagtacggc gacaccgagc cgggagaaga acaggtcgcc
    11701 gcggacctgg tcgacgacgg ccaggaccag gatcagcagc gacgcctcga tcccgaggat
    11761 cagccggtgg aactggagcg ccgaggcggc ccggcgggcc agcgccatgc ccgaggagcg
    11821 cggttcggac gccgactcct tgaccggcgg cagtccgccc tggtgccggg cgacaccgac
    11881 gaggtcggtc tccgccttga tcaggatcgc gccgagcgcg gccagcgtgc cgaggaacgc
    11941 ccagagccag tcgatgcggc cgctgcccca cgggtcggcg gcgcgcaggc cgaagccgac
    12001 gagcacggcg gcgtcggtga ggtacgcccc gacccggtcc aggtagaccc cgccgaggga
    12061 gtactgcttc ttccagcggg cgatctcgcc gtcgacgcag tccagcagca ggtacatctg
    12121 gacgcagacg acaccgagga cggcgcccgt gatccccggc accagcagcg ccggggcggc
    12181 gagcacgccg aagacggtca tcaggtacgt gagctggttg ggcgtgaccc tggtgttcac
    12241 caggtagcgg tcgacccgca gggacacctc acgcatgtag aggcgtccca tccagtgctc
    12301 accgctgcgc cggtccttca cccccggagg gtggacgacc ggtctgagtt cagctaccga
    12361 tggccttgac atagtcggcg tagatgtcct tgatctgctc ggtcttgagg tcgaggtgtt
    12421 cgatgatcgt gtaacggccg ggccgggtct cgggggcgaa ctccacggcg cggacgaact
    12481 cgtccaccgt gaagccgatg tccgtcggca ggaccggcag tccgtgccgt cgcagcacct
    12541 gggccatgta ggccgactcc tcgtgggctc cgcgcaggta catcgcgaag gccgcgccga
    12601 gtccgcactg ctcgccgtgg gcggctgccc gcttggggaa gagcaggtcg aaggcgtggt
    12661 tgatctcgtg gcaggcgccg gaggacgggc gggagtcgcc cgacaccgac atggcgatgc
    12721 cgctcagcac gagcgcctcg gccagcacct ggaggaagtc ggtgtccccg atgctgcccg
    12781 ggtggcgcag caccgcctcg ccggcctggc gggcgatcgc ggcggcgagg ccgtcgatct
    12841 tctcgccctt gacccggttg gcgagctccc agtccgcgat ggcggagatg ttggagacgg
    12901 cgtcgccgat gccggcccgt acgtagcgca ccggggcctc gcggatgacg tcgaggtcga
    12961 tcacgacggc gatcgggttc ggcaccccgt aggagccgcg cccggcgtcg ttgtcgaggg
    13021 tggcgaccgg ggagcacagg ccgtcgtggg cgaggttcgt cggcacggcg accaggggca
    13081 ggcccacccg cgccgcggcg aacttggcgc agtcgatgat cttgccgccg cccaggccga
    13141 cgaccgcgtc gaagtgcccg gcctttatgt cggcggccag ccggacggcg tcgtcgaggg
    13201 tgccgccgcc gacctcgtac caggtggcgc cgggcagggc cggggtgagg cgctcgcgca
    13261 gccgggcgcc cgagccgccg ctgacggcga gggcgagctt gcccgagtgc gagatgcgct
    13321 cgtcggcgag caccccgacc aggtcgtcga gggcacccgg gcggatgtcc acgacgaccg
    13381 gcgaggggat cagccgggtc agtactggca cgcgatctcc cgtccacggg cgagatcgtc
    13441 gtggttgtcg atctcgaccc actcgacgtc gccgatcggc gccacgtcga tccggaagcc
    13501 gcggttcacg agctcctggt agccgtgctc gtagaactgc tgcgggtcgg tctcccacac
    13561 ggtcctgagc gcgtccgcca gctcgggggc cgcgtcgccc tcgatcaggg tgacgccgat
    13621 gtactcgccg gtcgcctcgg cggggtccat cagcttggtg atcttcgtca tgcccttggc
    13681 ggggtcgacg acgaccttca tctcctcgtc cgcgagggac ttcacggtgt ccagcgcgag
    13741 gatgatccgc cggccctcac cgcgggcggc gagcagcgtc ttctccacgg agaccgggtg
    13801 cacggtgtcg ccgttggcga ggatcacccc gtccttgagg gcgtcacggc cgcaccacag
    13861 ggagtaggcg ttgttccact cctcggcctt gtcgttgtcg atgagggtca gcttgaggcc
    13921 gtacttcgcc tccagcgccg ccttgcgcgc gtacacggcc tccttgcggt agccgacgat
    13981 gaccgcgacc tccgtcagac cgatctcggc gaagttgccg agcgtcaggt cgagcaccgt
    14041 gggctcgccc tctatgcccg cgggccccac cggcaccagc gccttcggca ggctgtcggt
    14101 gtaggggcgc agacgccgtc cggcgccggc cgccagcacg aggccgatca tgcgggttct
    14161 ccttcatcgt gtacggaagg ggcgcctgcc cggtgggcgg cgacccagaa gcggatgctc
    14221 tcgacgagca ccacgacggc gacggccacg gcgaggaccg tgagcgcgac cgtgaactgc
    14281 gaggcggtga gcgccacggc cagcacggcg acgagcagcg tgcgcccgtc ctggcccccg
    14341 atggcgcgca ccagccaggc cggcggcgct ccggcgtcgc cgcggatgcg gtacaccgtg
    14401 tcgtagtgat ggtaggcgac ggcggcgacc agtccgaaag ccgcgggaag ggccccgtcg
    14461 gccccggcct tggcggcgag gatcaggacc gtgcagtact cgccggcccg gaagagcggc
    14521 gggaccagcc agtcgagggc gcccttgagg gggcgggcga cggcgtcggc gcagagcagg
    14581 acgtacagga cggcggccag caggagcgag aagcccggcc cgtcgaacgc ggccgcgagc
    14641 accagcagga cgacgccggc ggacgcgccg agcggcgccg ggatgcggat gccggccggg
    14701 agcctgcggg ccagcagctc ggtgagcggg ccgctgtccg cgaggtccgc cagcgcctgc
    14761 gccgcccggt ccgtacgccg ggccttgcgg gtgagcgagc gcaggacccg gcccgccgtg
    14821 gtgtaggtcg cggcgaacgc gcagccgacg agcagcgcgt agaaggtgat gcggggggtg
    14881 gtggcagcgg tgagaacggc gatcatggcc catcgttcgc cgatgggcag gactatcatc
    14941 cgccgcaccc agacggtcca gccgacgctg tcgagcttgt cggagagggc cgcggtgggg
    15001 ctggtgttgg cggtggcgtc gtggttggcc tcgttgaagg agaagtccac gacgtgccgg
    15061 caggtctgga ggaccatggc gccgagcgcc agggcccata cgtcgtcgcc gccccgggcg
    15121 gcgccgagcg cgaggcccgc gtagtaggcg tactccttgg cgcggtcgaa ggtggcgtcg
    15181 agccaggcgc cgagggtgga gtactggagg gagtaccggg cgagctggcc gtcggtgcag
    15241 tccaggacga acgacgcgat cagcaggacg cctgccgcga cgaagccgcc ccgggtgccg
    15301 gtggccgcgc aggccgccgc gatcagcgcg gtgatcagcg aggcggtggt gacctggttc
    15361 ggggtgaggc cgcggcgggc gcaccagcgg gccaggtagc gcgagtacgg gctgatgaag
    15421 tgggtggtga agaaaccgtc gcgggccttg acggccgact tcaggcggac ggcctcgtcg
    15481 tcgacggcgg cgacggcctg ccgtgcctcg ttgcgggcct gggggtcggc cgggacctcc
    15541 gcgaccaggc tgcccagctc ggggcggtgc acgtcggcgc cgtccgcgtc gagggcggtg
    15601 acgatccggt cggcgaggct gtcgacgtcc tccacgccgg ccgccgcccg cgcgggggcg
    15661 ctgccgtggg cggcactgcc aggggcggca ctgccgctcg ccgagttctc ccgggcgatc
    15721 gcgcgggtca gggcctgacg gccggccggc tgagcggtga ccgctcccgg gatcgcggcg
    15781 atcgggaagc ggggatcggt gagaccgaga cgcagcgcgt gctcgtggcc cacgaaacgc
    15841 gcgtccacga cggcgacgcg caggtctccg gggacggcgg cgaggagggc ctcggcttca
    15901 ccggcgtcgg aggcggtccg gacgtcgaag ccgagggacc gcaggtcgcc ctcgatcgac
    15961 gatccgggaa ccggcgagcc ggtgaggatg gcggtcggca accgaactca ctccctgggt
    16021 ccggcgcgtg cacgccggtc atgtacatgg tggaggcgcc cctgggccgc accggcgacc
    16081 tcaccggtgg ccgcgccagg cgggcggcat gtcggcagag gctatcggat gccgggaagg
    16141 ccgtgttcac cgcccgttca ggcacgatcg acgtgcatcc ggcagcacga cccggacccg
    16201 tccggaccgt cctcgccgcg atcatcatcg gtgatcggcg gcccgggcga caaaccgcac
    16261 cccccgggat ccgacatccc aggacatagc ccaccccgcc acggggcccg ccccagcgcg
    16321 ggcgccgacg gggccgggag gccgggggca cgggcccgac gcgggccggg gagcccggcg
    16381 cacgggcccg acacggggcc ggggagcccg gcgcacgggc gcgacgcggg gccggggcac
    16441 gggcccggac acgggccggg aggcccgggg cacgggcccg ggccgagagg gccggggcgc
    16501 gggcccgacg cgggccgggc agcccggcgc ccgggcgcgg acacgggccg agaggcccag
    16561 ggcaccgggc acggggcacg gcgggcgggc gcgggcccgg ggcacggtgg ggcgggctcc
    16621 cggcgccggc cggtgcgcgg gacgtgtgac gggacggtcg tgccgcttcc ccgggcatgg
    16681 ccgggactcc tctgacctgc ggttcgtttg cgcaggtcag ggcatatgac aacggtgttc
    16741 agtgccgcgt tgccggatac cccccacccg tagttgactg tggtcccggg gcagaacgcg
    16801 acggcccgcg acggaccaca gaagggctgc ttccatgggg gctgggcacg accacggcca
    16861 tacgcacgcg cacgtgccgg ccagcggtac ggccgcggcg gcgtaccggg gcaggctgcg
    16921 caccgcgctg tcgatcaccc tcggcgtcat ggtggtcgag atcgtcggcg gggtgctcgc
    16981 cgactccctc gcgctggtcg cggacgcggc gcacatggcg acggacgcgc tgggtctcgg
    17041 catggcgctg ctcgccatcc acttcgccaa ccgcccgccg agcgagaacc gcaccttcgg
    17101 gctggcccgc gccgagatcc tggccgccct ggcgaactgt ctgctgctgc tcggggtcgg
    17161 cggttacgtc ctgtacgagg ccgtccagcg gttcttcacg ccggcggcca ccgagggcgg
    17221 gctgatgatc tggttcggcg cgatcggcct ggtggcgaac atggtctccc tgtcgctgct
    17281 catgcgcggc caggcggaga gcctgaacgt gcgcgcggcc ttcctggagg tggcggccga
    17341 cgcgctgggc tcggtggcgg tgctgatctc cgcggtggtg atcctcacca ccggctggga
    17401 ggccgccgac ccgatcgcct cgctcgtgat cggcctgatg atcgtgccgc ggaccgtgaa
    17461 gctgctgcgc gagacactgg acgtgctgct ggagtcggcc cccaaggacg tcgacatggc
    17521 ggaggtgcgg tcccacgtcc tcgccctcga cggggtggag gacattcacg acctgcacgc
    17581 gtggacgatc acctccggga tgccggtgct gtcggcgcac gtggtggtcc gctcggacgt
    17641 cctgaacgcg atcggccacg agaagatgct gcacgagctc cagggctgcc tgggcgacca
    17701 cttcgacgtc gagcactgca ccttccagct ggagccgatc ggccacgcgg agcacgaggc
    17761 gcggctctgc cactgagccc gggcggccgc ggtccggccc ggtccgcaca ggcgttccgg
    17821 acggttcccc gtgcccggcg ccgggcaaga tcagctggcg ggggcccctg ccgggcgcgg
    17881 aacatgcgcg ctttcgtgtg aagtgccggg agtgccggac gcgtacggca gactggggac
    17941 gcgaggaccg atgttaagga tgggtatgcc gatcacacct gccaccgcga cacacacgtc
    18001 gtcgaacggc accgcggacg cgatcttgct ggagctggtc gacgaggacg gcgtcacgat
    18061 cggcaccgcg gagaagctcg ccgcccatca gccgccggga cagctgcacc gcgccttctc
    18121 ggtgttcctc ttcgacgagc gcggacggct gctgctccag cagcgggcgc tcggcaagta
    18181 ccactccccc ggcgtgtggt ccaacacctg ctgcggtcac ccctaccccg gtgaggcgcc
    18241 gttcgcggcg gcggcgcggc gcaccttcga ggagctgggg gtctccccca cgctgctcgc
    18301 cgaggcgggc acggtccgct acaaccaccc ggaccccgac tcgggcctgg tggagcagga
    18361 gtacaaccac ctcttcgtcg ggctggtgca gtcggcgccg cggccggacg cggacgaggt
    18421 cggcgacacg tccttcgtga cacccgccga gctggcggag ctgcacgcga agggcacgtt
    18481 ctcctcctgg ttcatgaccg tgctcgacgc ggcgcgcccg gcggtcagag aactgacggg
    18541 gc
//
