LOCUS       NZ_BMSI01000020        10405 bp    DNA     linear   CON 02-AUG-2024
DEFINITION  Streptomyces asoensis strain JCM 4185 sequence20, whole genome
            shotgun sequence.
ACCESSION   NZ_BMSI01000020
VERSION     NZ_BMSI01000020.1
KEYWORDS    WGS; RefSeq; STANDARD_DRAFT.
SOURCE      Streptomyces asoensis
  ORGANISM  Streptomyces asoensis
            Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
            Streptomycetaceae; Streptomyces.
REFERENCE   1
  AUTHORS   Wu,L. and Ma,J.
  TITLE     The Global Catalogue of Microorganisms (GCM) 10K type strain
            sequencing project: providing services to taxonomists for standard
            genome sequencing and annotation
  JOURNAL   Int J Syst Evol Microbiol 69 (4), 895-898 (2019)
   PUBMED   30832757
REFERENCE   2  (bases 1 to 164925)
  AUTHORS   Sun,Q. and Ohkuma,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-SEP-2020) Contact:Qinglan Sun Institute of
            Microbiology, Chinese Academy of Sciences, WFCC-MIRCEN World Data
            Centre for Microorganisms (WDCM); No. 3, yard 1, Beichen W Rd, Ao
            Yun Cun, Chaoyang, Beijing, Beijing 1000101, China URL
            :http://www.wdcm.org/
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: gcType assembly pipeline
            Genome Coverage                   :: 59X
            Sequencing Technology             :: Illumina HiSeq X Ten
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_014649075.1-RS_2024_08_02
            Annotation Date                   :: 08/02/2024 04:45:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 7,813
            CDSs (total)                      :: 7,736
            Genes (coding)                    :: 7,563
            CDSs (with protein)               :: 7,563
            Genes (RNA)                       :: 77
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 70
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 173
            CDSs (without protein)            :: 173
            Pseudo Genes (ambiguous residues) :: 0 of 173
            Pseudo Genes (frameshifted)       :: 53 of 173
            Pseudo Genes (incomplete)         :: 140 of 173
            Pseudo Genes (internal stop)      :: 10 of 173
            Pseudo Genes (multiple problems)  :: 28 of 173
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            ##antiSMASH-Data-START##
            Version                           :: 8.dev-cf2fc5ee(changed)
            Run date                          :: 2025-09-12 20:21:02
            NOTE :: This is a single region extracted from a larger record!
            Orig. start                       :: 81138
            Orig. end                         :: 91543
            ##antiSMASH-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            BMSI01000020.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     region          1..10405
                     /candidate_cluster_numbers="1"
                     /contig_edge="False"
                     /product="ectoine"
                     /region_number="1"
                     /rules="ectoine_synt"
                     /tool="antismash"
     cand_cluster    1..10405
                     /candidate_cluster_number="1"
                     /contig_edge="False"
                     /detection_rules="ectoine_synt"
                     /kind="single"
                     /product="ectoine"
                     /protoclusters="1"
                     /tool="antismash"
     protocluster    1..10405
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="False"
                     /core_location="[5000:5405](+)"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..5405
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="ectoine_synt"
                     /neighbourhood="5000"
                     /product="ectoine"
                     /protocluster_number="1"
     misc_feature    complement(161..175)
                     /note="TFBS match to ZuR_variant_1, Zinc-responsive
                     repressor, confidence: medium, score: 21.46"
                     /tool="antismash"
     gene            complement(538..1401)
                     /locus_tag="IE189_RS35345"
                     /old_locus_tag="GCM10010496_71860"
     CDS             complement(538..1401)
                     /codon_start=1
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1091: glutamine-binding lipoprotein glnH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130459.1"
                     /locus_tag="IE189_RS35345"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_71860"
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="WP_189927859.1"
                     /transl_table=11
                     /translation="MNTLPGRRNRVLAATTVAAGLMLVAACTSTDDGASGSKTAAGGVE
                     LVKAGQLTTCTHLPYPPFQSEIDGKVQGFDVSLIDLVAEDLGVKQVITDTPFENFKTGA
                     FLNSGQCDLAAAGMTITDERKKNVDFSDPYFDATQAVLVDKGAGVTSLADVKAKGVKLG
                     AQAQTTGEDYAKKQGFDPVSFESSDAVLNGLRTGQVKAVIIDYPVVQGWLKTKANADAF
                     KVVDNLNTGEQYGFTVKKGNTKLVAAINKALADARADGTYKKLYEKWIGPYDAAAASPS
                     AAASAS"
     gene            1607..2707
                     /locus_tag="IE189_RS35350"
                     /old_locus_tag="GCM10010496_71870"
     CDS             1607..2707
                     /NRPS_PKS="Domain: Aminotran_5 (74-300). E-value: 2.8e-26.
                     Score: 84.2. Matches aSDomain:
                     nrpspksdomains_IE189_RS35350_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010037833.1"
                     /locus_tag="IE189_RS35350"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_71870"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_189927936.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.8e-21, bitscore:
                     69.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTHPFLDLAPLSAAHFASIEDRVARLLDTRQDVVIMQGEALLPLE
                     GAIRGTAGPDTTALNVITGPYGKTFGDWLRDCGATVIDLAVPFHTAVTAAQVRQAFMEH
                     PEIDFVSLVHAEAATGNTNPVAEIGEVVREHGALFYLDAVASIGAEPVLPDAWGVDLCV
                     IGAQKAMGGPAGVSAVSVSERAWARMAANPGAPRRSYLSLLDWKERWVDGGRKALLHAP
                     AQLEMLALEACVERIEAVGPGTVMARHASAAAATRAGAVALGGGLEPYVHDAAEAAPVA
                     TTLRTPSGVVASDLVARALAGDPALPLAAGGGALAAEMIRVNHYGADATVGAVQGCLAA
                     LGAALAEQGRDVDLEAARRGAEDAWR"
     aSDomain        1829..2506
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS35350_Aminotran_5.1"
                     /evalue="2.80E-26"
                     /label="IE189_RS35350_Aminotran_5.1"
                     /locus_tag="IE189_RS35350"
                     /protein_end="300"
                     /protein_start="74"
                     /score="84.2"
                     /tool="antismash"
                     /translation="RDCGATVIDLAVPFHTAVTAAQVRQAFMEHPEIDFVSLVHAEAAT
                     GNTNPVAEIGEVVREHGALFYLDAVASIGAEPVLPDAWGVDLCVIGAQKAMGGPAGVSA
                     VSVSERAWARMAANPGAPRRSYLSLLDWKERWVDGGRKALLHAPAQLEMLALEACVERI
                     EAVGPGTVMARHASAAAATRAGAVALGGGLEPYVHDAAEAAPVATTLRTPSGVVASDLV
                     ARAL"
     misc_feature    complement(2909..2924)
                     /note="TFBS match to CRP, Cyclic AMP receptor protein,
                     confidence: weak, score: 15.8"
                     /tool="antismash"
     gene            3052..3558
                     /gene="ectA"
                     /locus_tag="IE189_RS35355"
                     /old_locus_tag="GCM10010496_71880"
     CDS             3052..3558
                     /EC_number="2.3.1.178"
                     /GO_function="GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /gene="ectA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006375120.1"
                     /locus_tag="IE189_RS35355"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_71880"
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="WP_189927860.1"
                     /transl_table=11
                     /translation="MTAAQADLRIDRPSVEDGAALWRLAKESGTLDLNSSYSYLLWCRD
                     FADTSVVARAEDGEPVGFVTGYVRPQDPHTLLVWQVAVDAAHRGRGLAAALLDGLTARL
                     AAVHPLTAVETTITPGNTASERLFTSYAERHGATVTREVLFGTGLFPDGPHDPEVLYRI
                     GPLSF"
     gene            3672..4943
                     /gene="ectB"
                     /locus_tag="IE189_RS35360"
                     /old_locus_tag="GCM10010496_71890"
     CDS             3672..4943
                     /EC_number="2.6.1.76"
                     /GO_function="GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /GO_function="GO:0047307 - diaminobutyrate-pyruvate
                     transaminase activity [Evidence IEA]"
                     /GO_process="GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /NRPS_PKS="Domain: Aminotran_3 (28-366). E-value: 3.9e-73.
                     Score: 238.3. Matches aSDomain:
                     nrpspksdomains_IE189_RS35360_Aminotran_3.1"
                     /codon_start=1
                     /gene="ectB"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1013: aminotransferase class-III"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007381147.1"
                     /locus_tag="IE189_RS35360"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_71890"
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="WP_189927861.1"
                     /sec_met_domain="Aminotran_3 (E-value: 6e-80, bitscore:
                     262.1, seeds: 13, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTITQPDLSVFETLESEVRSYCRGWPAVFDRAQGSRMYDEDGHRY
                     LDFFAGAGSLNYGHNNPVLKRALIDYLLRDGVTHGLDMSTVAKRSFLQTFQDLVLRPRD
                     LPYKVMFPGPTGTNAVEAALKLARKVKGREAIVSFTNAFHGMSLGSLAVTGNAFKRAGA
                     GVPLVHGTPMPFDNYFDGQVPDFLWFERLLEDQGSGLNKPAAVIVETVQGEGGINVARP
                     EWLRALKELCERQDMLLIVDDIQMGCGRTGAFFSFEEAGIVPDIVTVSKSISGYGLPMS
                     LCLFRPELDVWEPGEHNGTFRGNNPAFVTATAALEAYWADGSAMEKQTRARGEQIEQGL
                     ISITEENLADIKEYRGRGLVWGLEFHEKERAGRIARRAFELGLLIETSGPESEVVKLLP
                     ALTVTPEELDEGLSILARAVRETL"
     aSDomain        3756..4769
                     /aSDomain="Aminotran_3"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS35360_Aminotran_3.1"
                     /evalue="3.90E-73"
                     /label="IE189_RS35360_Aminotran_3.1"
                     /locus_tag="IE189_RS35360"
                     /protein_end="366"
                     /protein_start="28"
                     /score="238.3"
                     /tool="antismash"
                     /translation="FDRAQGSRMYDEDGHRYLDFFAGAGSLNYGHNNPVLKRALIDYLL
                     RDGVTHGLDMSTVAKRSFLQTFQDLVLRPRDLPYKVMFPGPTGTNAVEAALKLARKVKG
                     REAIVSFTNAFHGMSLGSLAVTGNAFKRAGAGVPLVHGTPMPFDNYFDGQVPDFLWFER
                     LLEDQGSGLNKPAAVIVETVQGEGGINVARPEWLRALKELCERQDMLLIVDDIQMGCGR
                     TGAFFSFEEAGIVPDIVTVSKSISGYGLPMSLCLFRPELDVWEPGEHNGTFRGNNPAFV
                     TATAALEAYWADGSAMEKQTRARGEQIEQGLISITEENLADIKEYRGRGLVWGLEFH"
     gene            5001..5405
                     /locus_tag="IE189_RS35365"
                     /old_locus_tag="GCM10010496_71900"
     CDS             5001..5405
                     /codon_start=1
                     /gene_functions="biosynthetic (rule-based-clusters)
                     ectoine: ectoine_synt"
                     /gene_kind="biosynthetic"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337952.1"
                     /locus_tag="IE189_RS35365"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_71900"
                     /product="ectoine synthase"
                     /protein_id="WP_189927862.1"
                     /sec_met_domain="ectoine_synt (E-value: 1.4e-54, bitscore:
                     176.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIVRSFKDIEGTDRHVRAASGTWESKRIVLAQERVGFSLHETILY
                     AGTETSMWYANHIEAVVCVEGDAELTDHESGLTHRITPGTMYLLDGHERHTLRVKEDFR
                     CICVFNPPVTGREDHDENGVYPLLTEPEEV"
     gene            5411..6298
                     /gene="thpD"
                     /locus_tag="IE189_RS35370"
                     /old_locus_tag="GCM10010496_71910"
     CDS             5411..6298
                     /EC_number="1.14.11.55"
                     /GO_function="GO:0016706 - 2-oxoglutarate-dependent
                     dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /gene="thpD"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) PhyH"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436210.1"
                     /locus_tag="IE189_RS35370"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_71910"
                     /product="ectoine hydroxylase"
                     /protein_id="WP_189927863.1"
                     /sec_met_domain="PhyH (E-value: 3.1e-39, bitscore: 128.7,
                     seeds: 39, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTALTDLYPTRGTTEVSVPRKDPVVWGSPDTPGPIPTAALQEYER
                     DGFLAVDQLIGDDEVAIYRRELERLVADPEIRADERAIVEPRSKEIRSVFEVHKLSQVF
                     AALVRDERVVGRARQILGSDVYVHQSRINVKPGFGAGGFYWHSDFETWHAEDGLPNMRT
                     VSVSIALTENLDTNGGLMIMPGSHRTFLGCAGATPEDNYKQSLQMQDAGTPSDEALTAM
                     AGDYGIRLFTGRAGSATWFDCNCMHGSGDNITPFPRSNVFIVFNSVENTAVEPFAAPIR
                     RPEFIGARDFTPVR"
     gene            complement(6383..7435)
                     /locus_tag="IE189_RS35375"
                     /old_locus_tag="GCM10010496_71920"
     CDS             complement(6383..7435)
                     /NRPS_PKS="Domain: Aminotran_5 (17-344). E-value: 1.1e-23.
                     Score: 75.7. Matches aSDomain:
                     nrpspksdomains_IE189_RS35375_Aminotran_5.1"
                     /codon_start=1
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139: aminotransferase class V"
                     /gene_kind="biosynthetic-additional"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020130466.1"
                     /locus_tag="IE189_RS35375"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_71920"
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="WP_189927864.1"
                     /sec_met_domain="Aminotran_5 (E-value: 2.5e-17, bitscore:
                     56.1, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="METFESLVRAEFTPKTTYLNTASNGLLPARTVAALHEAAVLRAEG
                     RPLTPLHEDTEACRAAYARLAGVPVTRVALGASVAAHTGVIAASLPAGAEVLTAEDDFT
                     SVLNPFHVRGDLKVRAVPLERLAESVRPGTALVAVSAAQSADGRVADLAALREAARAHG
                     ARTYVDHSQSAGWLPTDAGADDFSAAVSFKWLLGPHGAAFLVVPEDFGGLSPVLSGWVA
                     GERPWDSCYGPVAELARSARRFDLTHALFTYAGLRRSLELVEEIGVPAIAAHDLALADR
                     FRAGLAALGHRPVPAPGSAIVSVPGLGSRQPALADAGIQVSDRAGNLRASFHLYNTAAD
                     VDRLLTALSA"
     aSDomain        complement(6404..7384)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_IE189_RS35375_Aminotran_5.1"
                     /evalue="1.10E-23"
                     /label="IE189_RS35375_Aminotran_5.1"
                     /locus_tag="IE189_RS35375"
                     /protein_end="344"
                     /protein_start="17"
                     /score="75.7"
                     /tool="antismash"
                     /translation="YLNTASNGLLPARTVAALHEAAVLRAEGRPLTPLHEDTEACRAAY
                     ARLAGVPVTRVALGASVAAHTGVIAASLPAGAEVLTAEDDFTSVLNPFHVRGDLKVRAV
                     PLERLAESVRPGTALVAVSAAQSADGRVADLAALREAARAHGARTYVDHSQSAGWLPTD
                     AGADDFSAAVSFKWLLGPHGAAFLVVPEDFGGLSPVLSGWVAGERPWDSCYGPVAELAR
                     SARRFDLTHALFTYAGLRRSLELVEEIGVPAIAAHDLALADRFRAGLAALGHRPVPAPG
                     SAIVSVPGLGSRQPALADAGIQVSDRAGNLRASFHLYNTAADVDRL"
     misc_feature    complement(7472..7488)
                     /note="TFBS match to GnfM, Regulator of nitrogen-fixation
                     genes, confidence: weak, score: 19.76"
                     /tool="antismash"
     gene            complement(7533..8249)
                     /locus_tag="IE189_RS35380"
                     /old_locus_tag="GCM10010496_71930"
     CDS             complement(7533..8249)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020140791.1"
                     /locus_tag="IE189_RS35380"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_71930"
                     /product="DsbA family oxidoreductase"
                     /protein_id="WP_189927865.1"
                     /transl_table=11
                     /translation="MRVEIWSDIACPWCYVGKARFEKALEAFPYRDQVEVVHRSFELDP
                     GRAKGDVQPVLAMLTKKYGMSQAQAQSGEDNLGAQAAAEGLDYRTRDRDHGNTFDMHRL
                     LHFAKEHGRQDELIQILYRANFAEERSVFAEGDERLVELAAEAGLDAGAAREVLADPAR
                     YADEVRADEREAAELGANGVPFFVLDRAYGVSGAQPAEVFTQALTQAWGDRSPLKLIDD
                     GGAEACGPDGCAVPQH"
     gene            complement(8352..8855)
                     /locus_tag="IE189_RS35385"
                     /old_locus_tag="GCM10010496_71940"
     CDS             complement(8352..8855)
                     /EC_number="2.3.-.-"
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019329308.1"
                     /locus_tag="IE189_RS35385"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_71940"
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="WP_189927866.1"
                     /transl_table=11
                     /translation="MIGERFTGDRVIRTAVPAEAEAIAALHARARATYYPDGTPQDGTD
                     WLANWRLAVERPDGQVLCAVERGRIVALASFRPPQDAPAHTVKLFQFHVDPDHWRAGIG
                     TALHTACVEQWRAEGRRTAVLDVHVDNLRARAFYARQGWRPDEENPPAPDDHHLWLRYA
                     VPGE"
     gene            complement(8920..9528)
                     /locus_tag="IE189_RS35390"
                     /old_locus_tag="GCM10010496_71950"
     CDS             complement(8920..9528)
                     /codon_start=1
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016436202.1"
                     /locus_tag="IE189_RS35390"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /old_locus_tag="GCM10010496_71950"
                     /product="DUF1349 domain-containing protein"
                     /protein_id="WP_189927867.1"
                     /transl_table=11
                     /translation="MDVSLPELPFPLRTYGPDGHWSHEHGVLTGWAGARQDRFVPPTGD
                     GVDPAADAPRLLGAPEGDFQLIARVTVGFNWAFDAGALYVHVGERAWAKLCLEYSPDVA
                     TVCTVVTRGHSDDANSFTVEGSSVWLRLSRTGRAFAFHASRDGRRWTFVRLFTLGEEKE
                     TGAALVGFMTQSPMGEGCVVTYDHLEFRTEWPDDLRDGS"
ORIGIN
        1 cgcgcggcct ccagctggcc cttgggcacg gcctggatcc ccgcgcggaa cgtctccgcc
       61 atgtaggcgg ccccgatcag gccgagtccg agcgccgcct tgccgtacgt gccgccgacg
      121 atctcggtgc cggggaaggc cagcggcacg gccacgccga tgaacacgaa gatcagcagg
      181 gcgggcaggc cgcggaagat ctcgatgtag accccggcca cccagcggta cgggcccacg
      241 gaggacagcc gcatcagcgc gatgaccagg cccagcagca gtccgaggac gaagccggac
      301 accgtgtaca ggacggtgtt cttcagcgcc agggtgagga cgtccgggaa catctgccgg
      361 gcgatgtccc actgggcgaa ctggttcttc agccggcccc agtccgccgt ggtcgcgaag
      421 acgatcacgg cggccacgaa gacgacatac tgcgcgccgc gcgagagacg gcgcttctgc
      481 cgtcgggtga ggcccttttt cttcggctgg agccgtacgt cggtatcggc catggggtca
      541 ggaggccgag gcggcggccg acggggaggc ggccgcggcg tcgtaggggc cgatccactt
      601 ctcgtacagc ttcttgtagg tgccgtcggc cctggcgtcg gcgagcgcct tgttgatcgc
      661 ggcgaccagc ttcgtgttgc ccttcttcac cgtgaagccg tactgctcgc cggtgttgag
      721 gttgtcgacg accttgaagg cgtcggcgtt ggccttggtc ttcagccagc cctggacgac
      781 cgggtagtcg atgatgacgg ccttgacctg gccggtgcgc aggccgttga ggacggcgtc
      841 ggaggactcg aaggagacgg ggtcgaagcc ctgcttcttc gcgtagtcct cgccggtggt
      901 ctgcgcctgt gcgccgagct tgacgccctt ggccttcacg tcggcgagcg aggtgacgcc
      961 cgcgcccttg tcgaccagga cggcctgggt cgcgtcgaag taggggtcgg agaagtcgac
     1021 gttcttcttg cgctcgtcgg tgatggtcat gccggccgcg gccaggtcgc actgcccgga
     1081 gttgaggaag gcgccggtct tgaagttctc gaacggcgtg tcggtgatca cctgcttgac
     1141 gcccaggtcc tcggccacca ggtcgatcag ggacacgtcg aagccctgca ccttgccgtc
     1201 gatctccgac tggaacggag ggtagggcag gtgggtgcag gtggtgagct ggcccgcctt
     1261 gacgagctcg accccgccgg cggcggtctt ggagccgctg gcgccgtcat cggtggaggt
     1321 gcaggcggcg acgagcatga gcccggccgc cacggtggtg gcggccagga cgcggttccg
     1381 gcggccgggg agggtgttca cggcgggcct tcctgtaagg gaactatggg ttccgattat
     1441 aaggagatgt ttggggctct caaaccaaac cgatggtcac agggctctcg ggcctgggat
     1501 gtcaccggcc gggagggcgc gggtcgtcga tggggagggc ggggagggtg gggcgcgtgt
     1561 tggctagtct cgtcacctct accccgtgag tgaagagagc accgccgtga cacacccgtt
     1621 cctggacctc gccccgctga gtgccgcaca cttcgcctcg atcgaggacc gggtggcgcg
     1681 gctgctggac acccggcagg acgtcgtgat catgcagggc gaggcgctgc tgccgctgga
     1741 gggcgcgatc cgcggcacgg ccggtccgga cacgaccgcg ctgaacgtga tcaccggccc
     1801 gtacggcaag accttcgggg actggctgcg cgactgcggc gccacggtga tcgacctggc
     1861 ggtgcccttc cacacggcgg tgacggccgc acaggtccgg caggccttca tggagcaccc
     1921 ggagatcgac ttcgtgtcgc tggtgcacgc ggaggccgcg accggcaaca ccaacccggt
     1981 cgcggagatc ggcgaggtgg tgcgggagca cggcgcgctg ttctacctgg acgcggtggc
     2041 ctcgatcggc gcggagcccg tgctgccgga cgcgtggggg gtggacctgt gcgtgatcgg
     2101 cgcccagaag gcgatgggcg gacccgcggg cgtgtcggcc gtgtcggtga gtgagcgcgc
     2161 ctgggccagg atggcggcga accccggggc cccgcgccgc tcgtacctgt cgctgctgga
     2221 ctggaaggag cggtgggtcg acggcggccg caaggcgctg ctgcacgcac cggcgcagct
     2281 ggagatgctg gccctggagg cgtgcgtcga gcggatcgag gccgtggggc ccgggacggt
     2341 gatggcccgc cacgcgtccg cggccgccgc gacccgcgcg ggggcggtcg ccctgggcgg
     2401 cggtctggag ccgtacgtgc acgacgcggc agaggcggcg cccgtcgcca cgacgctgcg
     2461 gacgccgtcc ggggtggtgg cctcggacct ggtcgcccgg gccctggcgg gcgaccccgc
     2521 gctgccgctg gccgcgggcg ggggcgcgct ggccgcggag atgatccggg tcaaccacta
     2581 cggggccgac gccacggtgg gggcggtgca gggctgcctg gcggcgctcg gcgcggcgct
     2641 ggcggagcag gggcgcgacg tggacctgga ggcggcccgg cgcggcgccg aggacgcctg
     2701 gcgctgacgg cacggacaac cggccggacc ggcggcgcga cgacggcgac cggggaacca
     2761 tttcccggaa acgaacggac ggccgccgga atccgaattc aggaaaacag tgaattccct
     2821 ctccgatttc ccaccgatag cttgccctgg ctcttctgcc cggatttccc gcctctaaac
     2881 gcggagaatt tcgagcgacc tccccgtgcg tgacgcattg cacaagcagg aagatatgca
     2941 ccttttgcgc ggtgcaaaaa gggatgaaac ggcatcccgc cccttcggta tcacggaatc
     3001 accacggcct ctccatgcgt ttttgaattt cgctgggtaa gttcaagccg catgaccgcc
     3061 gcacaagcag acctgcgtat cgaccgcccg tcagtggagg acggagcagc actctggcgt
     3121 ctcgcgaagg aatccggaac gctcgacctg aactcctcct acagctatct gctgtggtgc
     3181 cgcgacttcg cggacacctc agtggtggca cgcgccgagg acggcgaacc ggtcggcttc
     3241 gtcaccgggt acgtccggcc gcaggacccg cacacgctgc tggtctggca ggtggccgtc
     3301 gacgccgcgc accgcggtcg cggtctcgcc gccgcgctgc tggacgggct gaccgcccgg
     3361 ctcgccgccg tacacccgct caccgccgtg gagaccacga tcacgccggg caacaccgcc
     3421 tccgagcggc tgttcacctc gtatgccgag cggcacgggg cgaccgtcac ccgcgaggtg
     3481 ctgttcggca ccgggctgtt ccccgacggt ccgcacgacc ccgaggtgct gtaccgcatc
     3541 gggccgctct ccttctgacg cccgcgcccg ccggccggcc ctcccccgcg cttccccccg
     3601 gcatccggct gccccaccgc gccgccccca cccgctctcc ttccgacccc acaccgagga
     3661 gtgttttcgt cgtgaccatc acccagcccg acctcagcgt cttcgagacc ctcgagtccg
     3721 aggtacgcag ctactgccgc ggctggcccg ccgtcttcga ccgcgcgcag ggcagccgca
     3781 tgtacgacga ggacggtcac cgctacctgg acttcttcgc cggggccggt tcgctcaact
     3841 acggccacaa caaccccgtg ctgaaacggg cgctgatcga ctacctgctg cgggacggcg
     3901 tcacgcacgg actcgacatg tcgaccgtcg ccaagcggtc cttcctccag accttccagg
     3961 atctggtgct gcgcccgcgc gacctgccgt acaaggtcat gttcccgggc ccgaccggca
     4021 ccaacgccgt ggaggccgcg ctgaaactgg cccggaaggt gaaggggcgc gaggccatcg
     4081 tgtcgttcac caacgccttc cacgggatgt ccctcgggtc gctcgccgtg acgggcaacg
     4141 cgttcaagcg ggccggtgcc ggcgtaccgc tggtgcacgg cacgcccatg ccgttcgaca
     4201 actacttcga cggccaggtg cccgacttcc tgtggttcga gcggctgctc gaggaccagg
     4261 gttccgggct caacaagccc gccgcggtga tcgtcgagac ggtccagggc gagggcggca
     4321 tcaacgtggc gcggcccgag tggctgcggg cgctcaagga gctgtgcgag cggcaggaca
     4381 tgctgctgat cgtcgacgac atccagatgg gttgcgggcg caccggcgcc ttcttctcct
     4441 tcgaggaggc cgggatcgtc ccggacatcg tgacggtctc caagtcgatc agcgggtacg
     4501 gactgcccat gtccctctgc ctgttccggc ccgagctgga cgtgtgggag ccgggcgagc
     4561 acaacggcac cttccgcggc aacaacccgg cgttcgtcac cgccaccgcc gccctggagg
     4621 cgtactgggc cgacggctcc gccatggaga agcagacccg ggcacgcggc gagcagatcg
     4681 agcaggggct catctcgatc accgaggaga acctcgccga catcaaggag taccgcgggc
     4741 gcggtctggt gtggggcctg gagttccacg agaaggagcg ggccggccgg atcgcccggc
     4801 gcgccttcga actgggcctg ctgatcgaga cgtcgggccc ggagagcgag gtcgtcaaac
     4861 tgctgccggc cctgaccgtg acccccgagg agctggacga gggcctcagc atcctcgccc
     4921 gcgccgtacg cgagaccctc tgacgccctc gcgcccgttc ccccttaccc cttcccctat
     4981 ctaggaggca tcgcagcacc gtgatcgtcc gttcgttcaa ggacatcgaa ggcaccgacc
     5041 ggcatgtgcg ggcggcgtcc ggcacctggg agagcaagcg catcgtgctc gcccaggaac
     5101 gggtcggatt ctcgctgcac gaaacgattc tgtacgccgg caccgagacg tcgatgtggt
     5161 acgcgaacca catcgaggcc gtcgtctgcg tcgagggcga cgccgagctg accgaccacg
     5221 agtccgggct cacgcaccgc atcacgcccg ggaccatgta cctcctcgac gggcacgagc
     5281 ggcacacgct ccgcgtcaag gaggacttcc gctgcatctg cgtgttcaac ccgcccgtca
     5341 cgggccggga ggaccacgac gagaacggcg tgtaccccct gctgaccgag cccgaggagg
     5401 tgtgagcgtc atgaccgcac tcaccgatct ctaccccacc cgcggcacca ccgaggtgtc
     5461 cgtgccccgc aaggaccccg tcgtctgggg ctccccggac acaccgggcc cgatcccgac
     5521 ggccgcgctc caggagtacg agcgcgacgg cttcctcgcc gtcgaccagc tgatcggcga
     5581 cgacgaggtg gcgatctacc ggcgtgagct ggagcggctc gtcgccgacc cggagatccg
     5641 ggcggacgag cgggcgatcg tcgaaccgcg ttccaaggag atcaggtccg tcttcgaggt
     5701 gcacaagctc agccaggtgt tcgccgcgct ggtccgtgac gagcgggtcg tcgggcgggc
     5761 ccggcagatc ctgggctcgg acgtgtacgt ccaccagtcg cggatcaacg tcaagccggg
     5821 cttcggggcc gggggcttct actggcactc cgacttcgag acctggcacg ccgaggacgg
     5881 gctgccgaac atgcggacgg tgtccgtgtc gatcgcgctg accgagaacc tggacaccaa
     5941 cggcgggctc atgatcatgc cgggctcgca ccggacgttc ctcggctgcg ccggggccac
     6001 gcccgaggac aactacaagc agtcgttgca gatgcaggac gcgggcaccc cgtcggacga
     6061 ggcgctgacc gcgatggcgg gcgactacgg catcaggctg ttcacgggca gggccggttc
     6121 ggcgacctgg ttcgactgca actgcatgca cggctcaggg gacaacatca cgccgttccc
     6181 gcgcagcaac gtgttcatcg tgttcaacag cgtggagaac acggccgtgg aaccgttcgc
     6241 ggcaccgatc cgccggccgg agttcatcgg cgcacgggat ttcacccccg tgcggtgagc
     6301 ccggccgctc gcttccccgg cgcgtgccgc cgggccagga cgcccccgcc ccgggtcagg
     6361 ggcgggggcg tcctggcgcc ggtcaggccg acagggcggt cagcagacgg tcgacgtcgg
     6421 ctgccgtgtt gtacaggtgg aaggaggcgc gcaggttgcc ggcgcggtcg gagacctgga
     6481 tgccggcgtc ggcgagagcg ggctgccggg agccgagtcc gggcaccgac acgatcgcgg
     6541 aaccgggggc cgggacgggc cggtggccga gcgcggcgag tccggcgcgg aagcggtccg
     6601 cgagggccag gtcgtgggcg gcgatcgccg gcacgccgat ctcctccacc agttccaggg
     6661 agcggcgcag tcccgcgtag gtgaacaggg cgtgcgtgag gtcgaaccgc cgtgccgagc
     6721 gggcgagttc ggccaccgga ccgtaacagc tgtcccaggg gcgctcgccc gcgacccatc
     6781 cggagagcac cggtgacagc ccgccgaagt cctcggggac cacgaggaag gccgccccgt
     6841 gcgggccgag cagccacttg aaggagacgg cggcggagaa gtcgtcggcg ccggcgtccg
     6901 tggggagcca gcccgcggac tgcgagtggt cgacgtaggt gcgggcgccg tgcgcgcggg
     6961 ccgcctcccg cagcgcggcc aggtcggcga cgcggccgtc ggcggactgg gcggcgctca
     7021 ccgcgaccag cgcggtgccc ggccggacgg actcggcgag ccgttccagc gggacggccc
     7081 gcaccttgag gtcgccgcgg acgtggaacg ggttcagcac cgaggtgaag tcgtcctcgg
     7141 cggtgagcac ttcggcgccc gcgggcagcg aggccgcgat cactccggtg tgcgcggcca
     7201 ccgaggcgcc cagcgccact cgggtgaccg ggaccccggc cagccgggcg taggcggcgc
     7261 ggcaggcctc ggtgtcctcg tgcagcgggg tcagcggcct gccctcggca cgcagcaccg
     7321 ccgcctcgtg cagggcggcg acggtgcggg ccggcagcag gccgttgctc gccgtgttca
     7381 ggtacgtggt cttcggggtg aactcggcgc ggacgaggct ctcgaaggtc tccatgggtc
     7441 cactgtggga cccctggaac tccccgtcca ttgccgattt ttacgtgatt ccgccaagga
     7501 ccgcttatgg atcggccctg acctgcggtt cctcagtgct gcggcaccgc gcacccgtcc
     7561 ggtccgcagg cctcggcacc gccgtcgtcg atcagcttca gcggggagcg gtcgccccac
     7621 gcctgggtga gggcctgggt gaagacctct gcgggctggg cgccggagac gccgtaggcg
     7681 cggtcgagga cgaagaaggg cacgccgttg gcgccgagct cggcggcctc gcgctcgtcg
     7741 gcgcggacct cgtcggcgta gcgggccggg tcggcgagga cctcgcgggc ggccccggcg
     7801 tcgagaccgg cctcggcggc cagctccacg agccgctcgt cgccctcggc gaagacggac
     7861 ctctcctcgg cgaagttggc gcggtagagg atctggatca gctcgtcctg ccggccgtgc
     7921 tccttggcga agtgcagcag gcggtgcatg tcgaaggtgt tgccgtggtc gcggtcccgg
     7981 gtgcggtagt cgaggccctc ggcggcggcc tgggcgccca ggttgtcctc gccggactgc
     8041 gcctgcgcct ggctcatccc gtacttcttg gtgagcatgg ccagcaccgg ctggacgtcg
     8101 cccttggcgc ggcccgggtc cagctcgaag gagcggtgca ccacctcgac ctggtcacgg
     8161 tacgggaagg cctcgagcgc cttctcgaag cgggccttgc ccacgtagca ccaggggcag
     8221 gcgatgtcgc tccagatctc gacgcgcatg tctcggcttt ctccaggtcg tgcgtgcggt
     8281 acggggctca cggccggaga cccgcacgga ggtttcctcc gccgggtgga tgaacgttca
     8341 agcagacggt ttcattcccc ggggaccgcg taccgcagcc agaggtggtg gtcgtcgggg
     8401 gcgggcgggt tctcctcgtc cggccgccag ccctggcggg cgtagaaggc ccgggcccgc
     8461 aggttgtcga cgtgcacgtc gaggacggcc gtgcgccgcc cctccgcccg ccactgctcg
     8521 acacaggccg tgtgcagggc cgtaccgatc ccggcccgcc agtggtcggg atcgacgtgg
     8581 aactggaaca gcttgacggt gtgcgcgggc gcgtcctgcg gcgggcggaa ggacgcgagg
     8641 gcgacgatcc gtccccgctc gacggcgcac agcacctggc cgtcgggccg ctcgacggcc
     8701 aaccgccagt tcgcgagcca gtccgtgccg tcctggggcg tcccgtccgg gtaataggtg
     8761 gcgcgggcgc gcgcgtgcag cgcggcgatc gcctccgcct cggcgggtac ggcggtacgg
     8821 atcacccggt cgccggtgaa gcgttctccg atcatgcaga ggaggacgtg gccacgcgct
     8881 ccccggttcc gggcacgggc gccgcccggc gggccgcgtt cagctcccgt cccgcaggtc
     8941 gtccggccac tcggtcctga actcgaggtg gtcgtaggtc accacgcatc cctcgcccat
     9001 cggggactgg gtcatgaagc cgaccagggc ggcgccggtc tccttctcct cccccagcgt
     9061 gaacagccgg acgaaggtcc agcgcctgcc gtcgcgggag gcgtggaagg cgaaggcgcg
     9121 gccggtcctg ctcagccgga gccagaccga acttccctcc acggtgaagg agttggcgtc
     9181 gtcggagtgg ccccgggtga ccaccgtgca gacggtggcc acgtccgggg agtactccag
     9241 gcacagcttg gcccaggccc gctcgccgac gtggacgtac aacgcccccg cgtcgaaggc
     9301 ccagttgaag ccgacggtga cgcgggcgat gagctggaag tcgccttccg gcgctcccag
     9361 cagccggggc gcgtcggcgg cggggtccac cccgtccccg gtgggcggca cgaaccggtc
     9421 ctgccgcgca ccggcccacc cggtgagcac cccgtgctcg tgggaccagt gcccgtcggg
     9481 cccataggta cgaaggggaa aaggcagttc gggaagcgag acatccatga cgtcatcaca
     9541 tcaccccacc ccggccgcac gacaaacccc ggacgagtca cccccccagg gtcgcgggaa
     9601 actgcgcgcc gggcccccgg gcacccctcg aaccccgggc gagccaaccc ccaggggcgc
     9661 ggggaactgc gcgccgagcc ccaacggacc cgcacgcacc ccccaccgca ccccggacgc
     9721 cgtccgaaca caccccggac ggcgtcccga agtccccatc cgcaccccag cggagcgcta
     9781 ccgctccaac cgcccgttga accgccgcgg cagccccagc tcgttctcgt cgcgcagctc
     9841 caccggcagc agcgccgcgg gggcgttctg gtaggcgacc ggccgcagcc accgctcgat
     9901 ggcggtcccg ccgaccgagg tggacgtcga ggtggtggcc gggtacggcc ccccgtggtg
     9961 ctgggccggc gcgacggcga cacccgtggg ccagccgttc accagcacgc gcccggcaag
    10021 cggggtcagt tcggcgagga tctcgccgcc ccggccctcg cccgcggcct cctcctcgga
    10081 cagctggacg gtcgcggtga ggttgccggg cagacgcgac agcaccgcct tgacctcgcc
    10141 ctcgtcgtcg tagcgcgcca cgacggtgac cgggccgaag cactcctcga gcagcaggtc
    10201 gtgcgcgccc tcggcggcca gccgggaggc cggcacggtc aggaagcccg cgctgaccgt
    10261 gtgctcgccg cccgcgccgg gcgtcaccgg cgactccacg tcgggcagcc cggcccgctc
    10321 ggcgacgccg gcgatgaagt tgtcgcgcat ccggtggtcg agcagcacgc ccgcgtcggt
    10381 gtcgctcacc gcgtcggtga gcgac
//
